KR101776439B1 - Methods for Screening Antimicrobial Agents against Geobacillus toebii - Google Patents

Abstract

TECHNICAL FIELD The present invention relates to a method of screening an antimicrobial agent capable of controlling microorganisms causing odor in an air conditioner, and a method of removing odor in an air conditioner. The odor-inducing microorganisms in the air conditioning system of the present invention can be used for the development of a novel antimicrobial agent or the development of a fragrance for blocking the odor by identifying the chemical properties of the metabolites of the microorganism. Further, the present invention has various industrial advantages such as being capable of being used in an air conditioner to preliminarily prepare an environment in which the microorganism can not be inhabited and to fundamentally remove the cause of the odor.

Description

Methods for Screening Antimicrobial Agents against Geobacillus toebii

TECHNICAL FIELD The present invention relates to a method of screening an antimicrobial agent capable of controlling microorganisms causing odor in an air conditioner, and a method of removing odor in an air conditioner.

Clean air is recognized as the basis for human health and well-being, and unpleasant odors or contaminated air act as a major factor impeding a pleasant environment. For example, unsatisfactory indoor air quality in enclosed conditions is caused by two important factors: One is the indoor air pollutant generated directly from the constituent material itself (building or vehicle) constituting the enclosed environment, and the other factor is the smell generated by the material generated by human activity or from the outside.

Air conditioning system is a system that optimizes the indoor environment by lowering indoor temperature by aiming to harmonize air temperature, humidity, air flow and cleanliness in buildings, vehicles, railways, ships, and aircraft. These air conditioning systems are increasing in penetration rate due to the improvement of living standards. There have been many improvements in the basic functions due to the increase of the air conditioning system, but there are still many problems to solve due to the environmental aspect for the indoor air quality.

In air conditioning systems, air-conditioner odor is known to be attributed to fungi and the metabolites of bacteria, but specific data on the types of fungi and bacteria and how the microorganisms specifically secrete certain metabolites have yet to be found It is in a state that it is not.

In the structure of the air conditioner, all the air passing through the blower passes through the evaporator core. When heat exchange is performed between the cold refrigerant and the air, condensation condensation occurs depending on the temperature difference on the evaporator core surface. Is provided with a good environment for fungi and bacteria to form and propagate. When fungi and bacteria are proliferated on the EVA core exposed to the outside air, volatile organic compounds (mVOCs) of the microorganisms are generated as the metabolites of the bacteria that have proliferated on the surface of the EVA core, and air passing through the EVA core is blown into the room The volatile organic compounds generated by the microorganisms may expose the room to odors caused by fungi and bacteria during long-term use.

The EVA core surface, which generates bad odors, is covered with biofilm according to long-term use. They are composed of bacteria, cell clusters and EPS. EPS is composed of protein, polysaccharide, polyuronic acid, Nucletic, and Lipid. On the surface of EVA core, various bacteria and fungi multiply as biofilm, and organic compounds (mVOCs) by microorganisms are emitted as metabolites. Many factors of odor are known as corruption.

Various types of fragrances are commercially available to remove the odor. However, it is often the case that the fungi and germs living in the EVA core can not be fundamentally removed, but merely temporarily serve to dilute an unpleasant smell. Antimicrobial agents have not yet been developed to act specifically on certain fungi or bacteria in the eva core, and are sold only because of the antimicrobial activity against common pathogens.

Accordingly, the present invention provides an antimicrobial agent capable of forming a pleasant indoor air environment by specifically isolating and preventing the fungi and bacteria of the eva core from being specifically blocked or preventing the propagation thereof, and a technique capable of removing unpleasant odors using the antimicrobial agent This is an urgent situation.

It should be understood that the foregoing description of the background art is merely for the purpose of promoting an understanding of the background of the present invention and is not to be construed as adhering to the prior art already known to those skilled in the art.

The present inventors have sought to identify microorganisms that live in an air conditioner and cause odor and to find a way to effectively control them. As a result, the present inventors completed the present invention by confirming that it is possible to successfully isolate six kinds of malodorous microorganisms forming a biofilm in the air conditioner, and to control the odor microbes effectively, thereby preventing the odor caused by the air conditioner.

Therefore, it is an object of the present invention to provide a microbial microorganism which is odor- to provide a screening method for the antimicrobial toebii).

Another object of the present invention is to provide a malodor generating microorganism in an air conditioner.

Another object of the present invention is to provide a method for inhibiting the growth of a malodor-inducing microorganism in an air conditioner, which comprises the step of applying or spraying an antibacterial agent in the air conditioner.

It is still another object of the present invention to provide a method of removing odor in an air conditioner, which comprises separating or killing odor-inducing microorganisms from the air conditioning apparatus.

It is still another object of the present invention to provide a method for removing odor in an air conditioner, which comprises suppressing the growth of odor-inducing microorganisms in the air conditioner.

Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention, claims and drawings.

According to one aspect of the present invention, the present invention provides a method for screening an antimicrobial agent for a microorganism that causes an odor in an air conditioner comprising the steps of:

(a) preparing said Geobacillus toebii or a culture thereof;

(b) contacting the sample to the Geobacillus compost or culture thereof;

(c) the geometry Bacillus Measuring inhibition of growth of compost ; And

(d) the geo Bacillus When the growth of the compost is inhibited, it said sample is a geo Bacillus Determining that the compost has an antibacterial activity that reduces the odor in the air conditioner in which the compost is generated.

The present inventors have sought to identify microorganisms that live in an air conditioner and cause odor and to find a way to effectively control them. As a result, it was confirmed that the six kinds of odor microorganisms forming the biofilm in the air conditioner were successfully separated and the odor caused by the air conditioner could be significantly blocked when they were controlled.

In the present specification, the term "air conditioner" is generally used to mean a system for comfortably maintaining temperature, humidity, cleanliness and flow of air in a space partially or entirely separated from the external environment. Preferable examples of the separated space may be an internal space partially or totally separated from the outside such as inside of a building or inside of a vehicle, a railroad, a ship, an aircraft, and the like. A preferable example of the air conditioner is an air conditioner.

In the air conditioner, all the air passing through the blower is passed through the evaporator core, and the condensation condensation phenomenon according to the temperature difference is continued on the surface of the evaporator core so that the environment becomes good for the growth of the microorganisms. (biofilm) is formed. At this time, the microorganisms metabolize various substances in the air, which are indoor and outdoor, as nutrients, and the odor is generated by the volatile organic compounds (mVOCs) generated as a result of metabolism.

Biofilm is a type of community surrounded by microorganisms, which is surrounded by a single membrane. The membrane protects microorganisms from external environment and supplies nutrients. Exopolymeric substances (EPS) are included in the membrane, and they contain various components such as proteins, polysaccharides, polyuronic acids, nucleic acids and lipids. On the surface of EVA cores, various microorganisms proliferate as their nutrients, So that the odor is discharged.

The present inventors isolated odor-causing microorganisms from the above-mentioned EVA cores. As a result of culturing these microorganisms, a dominant strain was isolated and cultured among the microorganisms forming the colonies. Methods for isolating and culturing the dominant strains can be carried out by various methods known in the art. For example, dominant microorganisms can be selected through a morphological approach such as dilution ratio, color, size and shape of colonies.

The dominant microorganism blood Brela Genus Microorganism, Chrysosobacterium Genus microorganism, spirosoma Genus microorganism, geobacillus Genus, or Feromonas Microorganism, preferably fibrillar Air Stu arena (Fibrella aestuarina), Cri Seo tumefaciens Geo-Force car Tampere (Chryseobacterium geocarposphaerae), spiro Soma ringgu Earl (Spirosoma linguale), spiro Soma Radio tolerance (Spirosoma radiotolerans), Geo Bacillus compost (Geobacillus toebii), or ferro Pseudomonas furanyl Quebec (Pelomonas puraquae ) .

The microorganisms are deposited on April 17, 2015 Date of the Korea Culture Center of Microorganisms were given the following accession numbers: blood Brela Air Stu arena (Fibrella aestuarina) HKMC-115 (Accession No: KCCM 11691P), Cri Seo tumefaciens Geo car force ferret (Chryseobacterium geocarposphaerae) HKMC-116 (Accession No: KCCM 11692P), spiro Soma Ringgu Earl (Spirosoma linguale) HKMC-117 (Accession No: KCCM 11693P), spiro Soma radio tolerance (Spirosoma radiotolerans) HKMC-114 (Accession No: KCCM 11690P), Bacillus Geo compost (Geobacillus toebii) HKMC-118 (Accession No: KCCM 11694P), Ferro Pseudomonas furanyl Quebec (Pelomonas puraquae ) HKMC-113 (Accession No .: KCCM 11689P).

These odor-inducing microorganisms have various industrial usefulness. For example, the present invention can be used to develop a novel antimicrobial agent capable of inhibiting the growth of the microorganism, and to develop a fragrance for blocking offensive odors by identifying the chemical properties of the metabolite of the microorganism. In addition, an environment in which the microorganisms can not be habituated in the air conditioner can be prepared in advance to fundamentally remove the cause of the odor.

The sample used in the antibiotic screening method of the present invention is to confirm whether or not the microorganisms have antimicrobial activity. For example, when pictures of antimicrobial activity in a particular sample chipping sealer's compost (Geobacillus toebii), the sample geometry Bacillus compost (Geobacillus toebii ) . < / RTI >

The antibacterial agent identified by the screening method of the present invention is preferably a Geobacillus compost toebii , and more preferably, it may have antimicrobial activity against other microorganisms in addition to the above microorganisms.

For example, some antimicrobial agents may have antimicrobial activity against all six microorganisms, and other antimicrobial agents may have no antimicrobial activity against one or some species. Also, antimicrobial activity of antimicrobial agents having antimicrobial activity against all of the six microorganisms may vary depending on the type of each microorganism (Table 8).

According to a preferred embodiment of the present invention, the antibacterial agent identified by the screening method of the present invention has antibacterial activity against Geobacillus toebii HKMC-118 (accession number: KCCM 11694P).

According to a preferred embodiment of the present invention, the sample to be screened includes a mixture of a biological compound, a compound and a biological preparation containing genetic information such as a mixture of a single compound and a compound, an extract of an animal or a plant, a nucleotide, a polypeptide and the like.

According to another aspect of the present invention, there is provided a malodor generating microorganism in an air conditioner.

The odor-inducing microorganism is preferably Geobacillus toebii , more preferably Geobacillus toebii HKMC-118 (accession number: KCCM 11694P).

According to still another aspect of the present invention, there is provided a method for inhibiting the growth of an odor-inducing microorganism in an air conditioner, comprising the step of applying or spraying an antibacterial agent in an air conditioner.

The antimicrobial agent usable in the present invention may be any antimicrobial agent which can be judged to have or have antibiotic activity against Geobacillus compost or microorganisms containing it. The antibacterial agent is applied or sprayed in the air conditioner to inhibit the growth of odor-induced Geobacillus compost or microorganisms containing the same, and the application or spraying may be carried out in a suspension in which a gas phase, a liquid phase, a gel phase, Can be made in various forms known in the art.

Further, the application or injection may be carried out over part or all of the inner surface or the inner structure of the air conditioner, and is preferably applied or sprayed onto the eva core in the air conditioner. Inhibition of growth is already geo Bacillus compost or microorganisms or coated or sprayed in a state forming a bio-film containing the same, geo Bacillus compost or be micro-organisms is applied or sprayed on the entire growth preventive forming a biofilm comprising the same It is possible.

According to still another aspect of the present invention, there is provided a method for removing odor in an air conditioner, comprising the step of applying or spraying an antibacterial agent in an air conditioner.

The smell removal of the present invention includes removal of all or a part of the smell, and includes coating or spraying before the generation of the malodor in order to prevent the smell.

Various microorganisms are growing in the air conditioner, and these microorganisms can be classified into microorganisms that cause bad odors and microorganisms that do not cause bad odors. Therefore, when the antibacterial agent specifically acts on microorganisms causing malodor, or has a growth inhibitory activity on all or a part of the dominant species among microorganisms causing malodour, all or part of the odor of the air conditioner is removed or improved .

According to still another aspect of the present invention, there is provided an air conditioning system comprising a gas- And separating or killing the compost from the air conditioning apparatus.

Separation or death of the present invention can be carried out by physical, chemical and biological methods in whole or in part of the microorganism or the microorganism containing the microorganism. As a physical method, the microorganism or the microorganisms including the microorganism can be artificially separated or killed by using a physical device. In chemical methods, the microorganism or the microorganisms containing the microorganism can be artificially separated or killed using an antibacterial or fungicide. And the biological method may be separated or killed by using a biological agent toxic to the microorganism or other microorganisms competing for survival with the microorganism, but the present invention is not limited thereto.

According to another aspect of the present invention, there is provided a method for removing odor in an air conditioner, comprising the step of suppressing the growth of the odor-causing microorganism, Geobacillus compost , in the air-conditioning apparatus.

The present invention provides a microorganism causing an odor in an air conditioner. The present invention also provides a method for screening an antimicrobial agent capable of controlling the microorganism. In addition, the present invention provides a method for controlling the microorganisms to remove odor in the air conditioner.

The odor-inducing microorganisms in the air conditioning system of the present invention can be used for the development of a novel antimicrobial agent or the development of a fragrance for blocking the odor by identifying the chemical properties of the metabolites of the microorganism. Further, the present invention has various industrial advantages such as being capable of being used in an air conditioner to preliminarily prepare an environment in which the microorganism can not be inhabited and to fundamentally remove the cause of the odor.

1 is a photograph showing a specimen sampled from an eva core of a malodorous used car.
FIG. 2 is a diagram illustrating a method of testing an antimicrobiality according to the present invention.
FIG. 3 is a view showing a state where a combination of high-purity odorless microorganisms is cultured using an aluminum pin, which is a material of Eva core.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Example  1: Odor induced Dominance  Selection of microorganisms

1. Securing odor vehicle and separation of air conditioner

The inventors of the present invention have found 10 kinds of used cars that smell bad odors in season (winter season: 2-3 months, summer season: 6-7 months) in order to identify the cause of odor generated in an enclosed environment such as a car interior (Fig. 1). Fig. 1 (a) is a graph showing the results of the experiment. Fig. 2 (a) is a graph showing the relationship between the biofilm and the biofilm.

No. Vehicle mileage season One 89,000 km Winter season
(2-3 months) 2 70,000 km 3 10,300 km 4 37,100 km 5 149,970 km 6 35,000 km Summer
(June-July) 7 28,000 km 8 42,000 km 9 110,000 km 10 90,000 km

2. Eva Core  Sample Sampling

The EVA core obtained from the used odors 1 to 10 was refrigerated at 4 ° C. until the EVA core sample was used, and then sealed with a polyethylene bag. In order to isolate and cultivate the microorganisms, 5 g of each sample was collected using a sterilized long nose plier at an arbitrary site including the front part and the rear part of each EVA core (FIG. 1).

3. Microbial Tally

The elimination of the microorganism from the sample collected from the EVA core proceeded through the following procedure:

① Mix the samples extracted from Eva core and put them in a stirrer.

(2) 200 ml of sterilized 1 X PBS (Phosphate Buffed Saline) is added to the agitator.

③ Mixed sample and PBS are stirred for 30 seconds.

④ Place the stirrer in ice for 1 minute.

⑤ Repeat steps 3 to 4 above twice.

⑥ Centrifuge the suspension at 13,000 rpm for 3 minutes at 4 ° C.

⑦ Take only the supernatant and transfer it to a new tube.

⑧ Wet the sterilized cotton swab with the supernatant and wipe the surface of the Eva core sample several times.

⑨ The wiped swab should be vortexed with only the head part in the supernatant.

⑩ Mix the precipitate obtained in ⑥ above with the mixture ⑨ and use it as the inoculum solution.

The above processes (1) to (10) were physically removed from the EVA cores mounted on the vehicle types 1 to 10 to isolate the microorganisms.

4. Separation of odor-inducing microorganisms and selection of dominant species

The separation of airborne germs is generally accomplished by heterotrophic culture of aerobic heterotrophic bacteria, commonly referred to as general germs. The bacterial isolates were cultured for 14 days at 28-30 ℃ using PTYG agar medium and R2A agar medium (PTYG agar medium was 0.25 g (Difco), 0.25 g (Difco), 0.2 g of Tryptone and 0.5 g of Yeast extract (Difco), Glucose 0.5 g (Difco), MgSO4 30 mg (Sigma), CaCl2 3 mg (Sigma) and Bacto agar 15 mg (Difco) were added to 980 ml of distilled water and adjusted to pH 7.0. (Difco), 0.5 g (Difco) of casamino acids, 0.5 g (Difco) of Dextrose, 0.5 g (Difco) of Soluble starch, and 0.5 g Add 0.1 g Sodium pyruvate (Difco), 0.3 g Dipotassium sulfate (Difco), 0.05 g Magnesium sulfate (Difco) and 15 g Bacto agar (Difco) in 980 ml distilled water and adjust the pH to 7.2 After sterilizing Kanamycin, Ampicillin and Chloramphenicol at a concentration of 100 ppm for the isolation of non-bacterium bacteria, the medium temperature was 50 ° C Inoculation was prepared by the antibiotic medium.

In order to separate and cultivate the dominant strains, several dominant strains were selected through a morphological approach such as dilution ratio, colony color, size and shape.

Separation Separate fungi and bacteria from the culture medium.

② Inoculate various bacterial strains of different morphology into a complex medium using a loop and isolate them pure.

③ Select the best growth medium among the inoculated medium and subculture.

④ The mold is separated from the end of the hypha using scalpel and then inoculated into the complex medium.

⑤ Select the medium that has the best growth among the medium inoculated with the fungal strain and subculture.

5. Dominance  Identification of microorganisms

For accurate identification of the isolated microorganism, 16s rRNA identification including the following steps was performed.

a) REP- PCR  Through pattern analysis Fingerprint (Fingerprints) survey

REP-PCR is a molecular biologic method for analyzing the structure of bacterial chromosomes. It is a fingerprinting method that distinguishes each bacterial strain from other bacteria. To perform REP-PCR, genetic characteristics were analyzed according to the following procedures.

(1) Cell lysis procedure

1) Transfer 2.5 μl of Lyse-N-Go PCR Reagent (Thermo) into PCR tube.

② Pipet the colonies on a clean bench and pipet into the upper tubes. Be careful not to get enough amount of solution to pour.

③ Cultivate in PCR machine according to manufacturer's instructions.

(2) PCR reaction

Using the components listed in Table 2 below, pre-denaturation was carried out at 93 ° C for 7 minutes, denaturation at 92 ° C for 1 minute, annealing at 51.5 ° C And denaturation, cooling, and extension at 65 ° C for 8 minutes in an extension step were repeated 33 times to perform PCR amplification.

1) dNTP (2.5 mM each) 12.5 μl ② Gitschier buffer 5.0 μl ③ DMSO (100%) 2.5 μl ④ Autoclaved 3 ^o DW 0.3 μl (5) BOXA1R primer (50 pmole / l)
5'CTACGGCAAGGCGACGCTGACG 1.0 μl ⑥ BSA (10 mg / ml) 0.4 μl ⑦ Bacterial DNA 2.5 μl ⑧ Taq polymerase (Roche) (5 U / ㎕) 0.8 μl

step 1 93 ℃ 7 min step 2 92 ° C 1 min step 3 51.5 DEG C 1 min step 4 65 ℃ 8 min step 2,3,4: additional 33 cycles step 6 65 ℃ 16 min step 7 4 ℃

(3) Gel electrophoresis

The DNA fragments amplified by each PCR were taken and 1.2 - 1.5% agarose gel with EtBr was used and 6 x dye was mixed with sample in 1: 5 ratio and as much as possible. Since most PCR products were between 100-1000 bp, a 100 bp ladder was loaded together and electrophoresed as slowly as possible (50 V) between the bromophenol blue and xylene cyanol dye to the middle of the entire gel. The same strain of the same DNA pattern on the gel was regarded as the same strain.

b) Air conditioning Dominance  16S of bacteria rRNA  Identification through gene analysis

The 16S rRNA (ribosomal ribonucleic acid) gene is used for identification of bacterial genomic classification and can be identified at the genus and species level of bacteria classified by REP-PCR.

(1) Cell lysis procedure

1) Transfer 5μl of Lyse-N-Go PCR Reagent (Thermo) into PCR tube.

② Pipet the colonies on a clean bench and pipet into the upper tubes. The amount of the solution should be such that the solution becomes slightly cloudy.

③ Lysate in a PCR machine according to the manufacturer's instructions (Table 4).

Cycle Temperature (° C) Time (seconds) One 65 30 2 8 30 3 65 90 4 97 180 5 8 60 6 65 180

(2) 16S rRNA gene PCR

PCR conditions (Total 50 ㎕): The remaining solutions except for DNA and Taq were mixed as necessary as shown in Table 5 below, and 44.5 ㎕ was added to the above lysis solution. Denaturation at 94 ° C for 1 minute, denaturation at 94 ° C for 1 minute, annealing at 55 ° C for 1 minute, extension step (extension) as described in Table 6, pre-denaturation at 94 ° C for 5 minutes, denaturation at 94 ° C for 1 minute, ) At 72 DEG C for 1 minute and 30 seconds, and denaturation, cooling and extension steps were performed 29 times to perform PCR amplification.

Autoclaved 3 ^o DW 22 μl 10xbuffer (Roche) 5 μl dNTP (Roche, 2.5 mM) 5 μl DMSO 5 μl BSA (10 mg / ml) 2.5 μl 27 mf (20 pmoles / l) 2.5 μl 1492 r (20 pmole / l) 2.5 μl DNA 5 μl Taq (Roche) 0.5 μl

step 1 94 ° C 5 min step 2 94 ° C 1 min step 3 55 ° C 1 min step 4 72 ℃ 1 min 30 sec Go to step 2: additional 29 cycles step 6 72 ℃ 10 min step 7 4 ℃ hold

(3) PCR purification

The 16S rRNA gene PCR product was purified using the Qiaquick PCR purifcation kit according to the following procedure.

① Put 5 times PB buffer of PCR product.

② Divide the mixed solution in the QIAquick column.

③ Centrifuge for 1 minute to bind DNA and remove the mixed solution.

④ Add 750 μl of PE buffer to the QIAquick column for wash, centrifuge for 1 minute and remove the mixed solution.

⑤ Centrifuge again for 1 minute.

⑥ Move the QIAquick column to a new tube.

⑦ To extract DNA, add 30 μl of EB buffer and leave for 1 minute.

⑧ Centrifuge for 1 minute to collect the DNA dissolved in EB in the tube.

As a result of the above-mentioned experiment, in order to confirm whether or not the odor of the purely isolated microorganism was generated, the sensory evaluation was carried out by cultivating in the following manner:

① Pure separation Separate the cultured microorganisms into liquid nutrient medium.

② The inoculated medium is incubated at 28 ℃ for 5-7 days.

③ Inoculate the solid nutrient medium with 100 ㎕ of cells cultured in liquid medium.

④ Spread the inoculated cells evenly using a spreader.

⑤ Seal the Petri dishes and incubate at 28 ℃ for 10 days.

The sensory evaluation was carried out using a panel of seven persons, and the odor-causing microorganisms were selected using the average value after evaluating the odor intensity using the 5-point scale method. Through the identification by the 16S rRNA gene analysis, And they were deposited on April 17, 2015 at the Korea Microorganism Conservation Center.

No. Identification number Microorganism name Accession number One HKMC-113 Feromonas Puraqua
( Pelomonas puraquae ) KCCM  11689P 2 HKMC-114 Spirosoma Radio Tolerance
( Spirosoma radiotolerans ) KCCM  11690P 3 HKMC-115 Fibrela Air Stu Arena
( Fibrella aestuarina ) KCCM  11691P 4 HKMC-116 Cryptobacterium Geocafos Ferre
( Chryseobacterium geocarposphaerae ) KCCM  11692P 5 HKMC-117 Spirosoma Ringgold
( Spirosoma linguale ) KCCM  11693P 6 HKMC-118 Geo Bacillus  compost
( Geobacillus toebii ) KCCM  11694P

Example  2: Evaluation of antibacterial activity against selected odor-inducing microorganisms

1. Experimental process

The present inventors evaluated antimicrobial activity against the dominant microorganisms selected in Example 1 using various commercially available antimicrobial agents. The antibacterial agents used in the present invention are as follows:

A Antimicrobial agent: Kim Care (Yuhan-Kimberly)

B Antimicrobial agent: Pebble (P & G)

C Antimicrobial agent: Mass production antimicrobial agent containing 45 to 50% of methyl alcohol, 1 to 5% of chromium sulfate (CAS 10101-53-8), 1 to 5%

The evaluation of antibacterial activity was carried out through the following steps:

① Preparation of sterilized filter paper

② Preparation of three kinds of antimicrobial agents (Control group: no antimicrobial agent, experimental group: antimicrobial agent A, antimicrobial agent B, antimicrobial agent C)

③ Put filter paper in antibacterial agent

④ Apply each odor-inducing microorganism to the nutrient medium

⑤ Put the filter paper containing each antimicrobial agent in the nutrient medium to which odor-inducing microorganism is applied

⑥ Culture at 28 to 30 ℃ for 5 days

⑦ Measurement of growth inhibition zone

The growth inhibition zone was measured by using a vernier caliper to measure the diameter of the growth inhibition zone. The specific method is as shown in FIG.

2. Experimental results

The mean values of the diameters of the growth inhibition zones measured by the above-mentioned method for each of 6 kinds of odor-inducing microorganisms were shown in Table 8.

No. Microorganism name ( Depositor ) Antimicrobial agent  No A B C One Pelomonas puraquae HKMC-113 0 1.17 2.07 3.93 2 Spirosoma radiotolerans HKMC-114 0 2.03 2.17 4.57 3 Fibrella aestuarina HKMC-115 0 2.13 2.63 4.30 4 Chryseobacterium geocarposphaerae HKMC-116 0 0.97 1.57 2.70 5 Spirosoma linguale HKMC-117 0 1.83 2.10 4.83 6 Geobacillus toebii strain R-35642 HKMC-118
0 1.73 1.93 2.20

(Unit: cm)

As it is shown in Table 8, in the case of antimicrobial agents A Cri Seo tumefaciens Geocafos Ferre , The antimicrobial activity was relatively weak, and in the case of antimicrobial agent B , The strong antimicrobial activity with respect to the air side or Stu arena, Cri Seo tumefaciens Geocafos Ferre It was found that the antimicrobial activity was weak.

In case of the antimicrobial agent C, the antimicrobial activity against Geobacillus compost is relatively weak, but the antimicrobial agents A and B And it showed high antibacterial activity.

Example  3: odor-inducing microorganism removed On Eva Core  Odor evaluation for

In order to reproduce the eva core in which the bad odor microorganisms have been removed or separated, the present inventors have used a combination of odorless microorganisms excluding the bad odor microorganisms of Example 1 among the dominant microorganisms living in the eva cores by using an aluminum pin (Table 9, Fig. 3).

The odorless microorganisms were selected as the dominant species which did not cause malodour among the microorganisms forming colonies during culturing among the microorganisms in the above Eva core. The culture method was as follows.

① Pure isolation Separately cultivated microorganisms are inoculated into R2A medium.

② The inoculated medium is incubated at 28 ℃ for 5-7 days.

③ Prepare an aluminum pin (sterilized at 121 ℃ for 20 minutes).

④ Immerse each antimicrobial agent so that the surface is evenly coated.

⑤ Place the coated aluminum fins on the Patri dish.

⑥ Discard the supernatant by centrifuging 1 ml of the inoculated microorganism culture.

⑦ Add 1 ml of sterilized 1 x PBS and centrifuge again.

⑧ Repeat ⑦ above twice.

⑨ Add 100 μl of odorless microorganism washed with PBS to the middle of aluminum pin.

⑩ Inoculate on prepared aluminum fin and dry at room temperature.

⑪ Seal the Patry Dish and incubate at 28 ℃ for 1 month.

As a result, it was found that after one month, the combination of the following Table 9 (the smell result in the culture of the eva-core-habitat microorganism from which the malodorous microorganism was removed) was found to be odorless.

Combination microbe Odor evaluation after 1 month One Methylobacterium brachiatum Odorless 2 Methylobacterium platani Odorless 3 Methylobacterium aquaticum  + Methylobacterium platani Odorless 4 Methylobacterium platani  + Methylobacterium brachiatum Odorless 5 Methylobacterium aquaticum  + Methylobacterium platani  + Methylobacterium brachiatum Odorless 6 Methylobacterium aquaticum  + Methylobacterium platani  + Methylobacterium  brachiatum + Acinetobacter johnsonii Odorless 7 Methylobacterium aquaticum  + Methylobacterium platani  + Methylobacterium  brachiatum + Bacillus vietnamensis Odorless 8 Methylobacterium aquaticum  + Methylobacterium platani  + Methylobacterium  brachiatum + Brevibacillus invocatus Odorless 9 Methylobacterium aquaticum  + Methylobacterium platani  + Methylobacterium  brachiatum + Deinococcus ficus Odorless 10 Methylobacterium aquaticum  + Methylobacterium platani  + Methylobacterium  brachiatum + Leifsonia left Odorless 11 Methylobacterium aquaticum  + Methylobacterium platani  + Methylobacterium  brachiatum + Methylobacterium komagatae Odorless 12 Methylobacterium aquaticum  + Methylobacterium platani  + Methylobacterium  brachiatum + Pseudomonas nitroreducens Odorless 13 Methylobacterium aquaticum  + Methylobacterium platani  + Methylobacterium  brachiatum + Sphingomonas aquatilis Odorless 14 Sphingomonas aquatilis  + Brevibacillus  invocatus Odorless 15 Leifsonia left  + Methylobacterium  komagatae Odorless 16 Acinetobacter johnsonii  + Sphingomonas  aquatilis + Methylobacterium komagatae Odorless 17 Pseudomonas nitroreducens Odorless 18 Acinetobacter johnsonii  + Pseudomonas nitroreducens Odorless 19 Brevibacillus invocatus  + Acinetobacter  johnsonii + Pseudomonas nitroreducens Odorless 20 Leifsonia left  + Pseudomonas nitroreducens Odorless 21 Brevibacillus invocatus  + Sphingomonas  aquatilis + Pseudomonas nitroreducens Odorless 22 Acinetobacter johnsonii  + Sphingomonas  aquatilis + Pseudomonas nitroreducens Odorless 23 Methylobacterium aquaticum  + Methylobacterium komagatae  + Bacillus vietnamensis + Deinococcus ficus Odorless 24 Methylobacterium aquaticum  + Methylobacterium komagatae  + Curtobacterium  flaccumfaciens + Deinococcus apachensis  + Bacillus subtilis subsp . subtilis Odorless 25 Methylobacterium aquaticum  + Methylobacterium komagatae  + Spirosoma  linguale + Sphingomonas dokdonensis  + Leifsonia left Odorless

As a result of the above experiment, when the malodor generating microorganisms in the air conditioner are removed by chemical or physical methods to form only the microorganisms which do not cause malodor, the odor generated in the air conditioner is significantly .

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Korea Microorganism Conservation Center KCCM11689P 20150417 Korea Microorganism Conservation Center KCCM11690P 20150417 Korea Microorganism Conservation Center KCCM11691P 20150417 Korea Microorganism Conservation Center KCCM11692P 20150417 Korea Microorganism Conservation Center KCCM11693P 20150417 Korea Microorganism Conservation Center KCCM11694P 20150417

<110> Hyundai Motor Company <120> Method for Screening Antimicrobial Agents against Pelomonas          puraquae <160> 6 <170> Kopatentin 1.71 <210> 1 <211> 1460 <212> DNA <213> Pelomonas puraquae HKMC-113 <220> <221> rRNA &Lt; 222 > (1) .. (1460) <223> 16s rRNA <400> 1 tggctcagat tgaacgctgg cggcatgcct tacacatgca agtcgaacgg taacaggtta 60 agctgacgag tggcgaacgg gtgagtaata tatcggaacg tgcccagtcg tgggggataa 120 ctgctcgaaa gagcagctaa taccgcatac gacctgaggg tgaaagcggg ggatcgcaag 180 acctcgcgcg attggagcgg ccgatatcag attaggtagt tggtggggta aaagcctacc 240 aagccaacga tctgtagctg gtctgagagg acgaccagcc acactgggac tgagacacgg 300 cccagactcc tacgggaggc agcagtgggg aattttggac aatggacgca agtctgatcc 360 agccatgccg cgtgcgggaa gaaggccttc gggttgtaaa ccgcttttgt cagggaagaa 420 aaggttctgg ttaatacctg gaactcatga cggtacctga agaataagca ccggctaact 480 acgtgccagc agccgcggta atacgtaggg tgcaagcgtt aatcggaatt actgggcgta 540 aagcgtgcgc aggcggttat gcaagacaga tgtgaaatcc ccgggctcaa cctgggaact 600 gcatttgtga ctgcatggct agagtgcggc agagggggat ggaattccgc gtgtagcagt 660 gaaatgcgta gatatgcgga ggaacaccga tggcgaaggc aatcccctgg gcctgcactg 720 acgctcatgc acgaaagcgt ggggagcaaa caggattaga taccctggta gtccacgccc 780 taaacgatgt caactggttg ttgggagggt ttcttctcag taacgtagct aacgcgtgaa 840 gttgaccgcc tggggagtac ggccgcaagg ttgaaactca aaggaattga cggggacccg 900 cacaagcggt ggatgatgtg gtttaattcg atgcaacgcg aaaaacctta cctacccttg 960 acatgtctgg aatcctgaag agatttggga gtgctcgaaa gagagccaga acacaggtgc 1020 tgcatggccg tcgtcagctc gtgtcgtgag atgttgggtt aagtcccgca acgagcgcaa 1080 cccttgtcat tagttgctac gaaagggcac tctaatgaga ctgccggtga caaaccggag 1140 gaaggtgggg atgacgtcag gtcatcatgg cccttatggg tagggctaca cacgtcatac 1200 aatggccggg acagagggct gccaacccgc gagggggagc taatcccaga aacccggtcg 1260 tagtccggat cgtagtctgc aactcgactg cgtgaagtcg gaatcgctag taatcgcgga 1320 tcagcttgcc gcggtgaata cgttcccggg tcttgtacac accgcccgtc acaccatggg 1380 agcgggttct gccagaagta gttagcctaa ccgcaaggag ggcgattacc acggcagggt 1440 tcgtgactgg ggtgaagtcg 1460 <210> 2 <211> 1451 <212> DNA <213> Spirosoma radiotolerans HKMC-114 <220> <221> rRNA <222> (1). (1451) <223> 16s rRNA <400> 2 tttgatcatg gctcaggatg aacgctagcg gcaggcctaa tacatgcaag tcgaacgggt 60 cgcaagacca gtggcaaacg ggtgcgtaac gcgtaagcaa cctgcctcat actgggggat 120 agcccggcga aagctggggt aaacccgcac ggtcccctga actcacctgg gttttggggt 180 aaacatttat gggtatgaga ggggcttgcg tctgattagt tagttggcag ggtaacggcc 240 taccaagacg atgatcagta ggggttctga gaggattggc ccccacatgg gtactgagat 300 acggacccaa ctcctacggg aggcagcagt agggaatatt gggcaatgga ggcaactctg 360 acccagccat gccgcgtgca ggatgaaggc gctcagcgtt gtaaactgct tttatcgatg 420 aagaactgta gatctgcgga tttatttgac ggtaatcgag gaataagcac cggctaactc 480 cgtgccagca gccgcggtaa tacggagggt gcgagcgttg tccggattta ttgggtttaa 540 agggtgcgta ggtgggatcg taagtctgat ttgaaagcag gcggcttaac cgtctgatgt 600 ggttggaaac tgcggttctt gaatgggttg gcggtagccg gaatgggtca tgtagcggtg 660 aaatgcatag atatgacccg gaacaccgat tgcgaaggca ggctactacg acttgattga 720 cactgaggca cgagagcatg ggtagcgaac aggattagat accctggtag tccatgccgt 780 aaacgatgat tactggctgt ttgcccgata gggtgagtgg ctgagcgaaa gcgttaagta 840 atccacctgg ggagtacgct ggcaacagtg aaactcaaag gaattgacgg gggtccgcac 900 aagcggtgga gcatgtggtt taattcgatg atacgcgagg aaccttacct gggctagaat 960 gtgcgtgaag gtatcagaaa tggtgccgtg tagcaataca cacaaaacaa ggtgctgcat 1020 ggctgtcgtc agctcgtgcc gtgaggtgtt gggttaagtc ccgcaacgag cgcaacccct 1080 gtacttagtt gccagcatgt aatgatgggc actctaagta gactgcctgc gcaagcagag 1140 aggaaggagg ggacgacgtc aagtcatcat ggcccttacg cccagggcga cacacgtgct 1200 acaatggtcg gtacagcggg tcgctacaca gtaatgtgat gccaatcttg taaagccggt 1260 cacagttcgg attggggtct gcaacccgac cccatgaagc tggaatcgct agtaatcgcg 1320 catcagccat ggcgcggtga atacgttccc ggaccttgta cacaccgccc gtcaagccat 1380 gggagttggg gggacctgaa ggtcggtatg atagccgggc aagggtaaac tcggtaacta 1440 gggctaagtc g 1451 <210> 3 <211> 1457 <212> DNA <213> Fibrella aestuarina HKMC-115 <220> <221> rRNA &Lt; 222 > (1) .. 1457 <223> 16s rRNA <400> 3 tttgatcatg gctcaggatg aacgctagcg gcaggcctaa tacatgcaag tcgagcgggt 60 agcaatacca gcggcaaacg ggtgcgtaac gcgtaaataa cctgccctca actgggagat 120 agctttgcga aagcggaggt aataccccat agtcttcttg gttcacctgg actgattagt 180 aaagcagcaa tgtggttgag gagggatttg cgtctgatta gttagttggc agggtagtgg 240 cctaccaaga cgatgatcag tcggggctct gagaggaggg tcccccacat gggcactgag 300 acacgggccc aactcctacg ggaggcagca gtagggaata ttgggcaatg ggcggaagcc 360 tgacccagcc atgccgcgtg ccggatgaag gccctctggg ttgtaaacgg cttttatctg 420 ggaagaagag cagggatgcg tccctgtgtg acggtaccag aggaatcagc accggctaac 480 tccgtgccag cagccgcggt aatacggagg gtgcaagcgt tgtccggatt tattgggttt 540 aaagggtgcg taggtggcct gataagtcag ctttgaaagt ggcttgctta acaagacagg 600 gtgggttgat actgtcaggc ttgaatggga tggaggttac tggaacgggt cgtgtagcgg 660 tgaaatgcat agatatgacc cagaactcca attgcgaagg caggtggcta catcccgatt 720 gacactgagg cacgagagca tggggagcaa acaggattag ataccctggt agtccatgcc 780 gtaaacgatg ataactgact gtgtgatttt cggattgcgt ggttaagcga aagcgttaag 840 ttatccacct ggggagtacg ccggcaacgg tgaaactcaa aggaattgac gggggtccgc 900 acaagcggtg gagcatgtgg tttaattcga tgatacgcga ggaaccttac ccggattaga 960 atgcgcgtga agtactcaga gatgggtacg tccagcaatg gacacaaagc aaggtgctgc 1020 atggctgtcg tcagctcgtg ccgtgaggtg ttgggttaag tcccgcaacg agcgcaaccc 1080 ctggaatcag ttgccagcac gtcaaggtgg ggactctggt tcgactgcct gcgcaagcag 1140 agaggaaggc ggggacgacg tcaagtcatc atggccctta catccggggc gacacacgtg 1200 ctacaatggc cggtacagcg ggtcacgatc ccgcaagggg gagtcaatct cagcaaagcc 1260 ggtcacagtt cggattgggg tctgcaactc gaccccatga agctggaatc gctagtaatc 1320 gcgcatcagc catggcgcgg tgaatacgtt cccggacctt gtacacaccg cccgtcaagc 1380 catgggagtc ggggggacct gaagcggggt gtcacatccc ttcaagggta aatctggcga 1440 ctggggctaa gtcgtaa 1457 <210> 4 <211> 1457 <212> DNA <213> Chryseobacterium geocarposphaerae HKMC-116 <220> <221> rRNA &Lt; 222 > (1) .. 1457 <223> 16s rRNA <400> 4 tggctcagga tgaacgctag cgggaggcct aacacatgca agctgagcgg tagagttcct 60 tcggggactt gagagcggcg tacgggtgcg gaacacgtgt gcaacctgcc tttatcaggg 120 ggatagcctt tcgaaaggaa gattaatacc ccataatata attgatggca tcattgatta 180 ttgaaaactc cggtggataa agatgggcac gcgcaagatt agatagttgg tgaggtaacg 240 gctcaccaag tcgatgatct ttagggggcc tgagagggtg atccccccac actggtactg 300 agacacggac cagactccta cgggaggcag cagtgaggaa tattggacaa tgggttagcg 360 cctgatccag ccatcccgcg tgaaggacga cggccctatg ggttgtaaac ttcttttgta 420 cagggataaa cctttccacg tgtggaaagc tgaaggtact gtacgaataa gcaccggcta 480 actccgtgcc agcagccgcg gtaatacgga gggtgcaagc gttatccgga tttattgggt 540 ttaaagggtc cgtaggcgga tctgtaagtc agtggtgaaa tctcacagct taactgtgaa 600 actgccattg atactgcagg tcttgagtaa ggtagaagtg gctggaataa gtagtgtagc 660 ggtgaaatgc atagatatta cttagaacac caattgcgaa ggcaggtcac tatgtcttaa 720 ctgacgctga tggacgaaag cgtggggagc gaacaggatt agataccctg gtagtccacg 780 ctgtaaacga tgcttactcg tttttgggct ttcgggttca gagactaagc gaaagtgata 840 agtaagccac ctggggagta cgaacgcaag tttgaaactc aaaggaattg acgggggccc 900 gcacaagcgg tggattatgt ggtttaattc gatgatacgc gaggaacctt accaaggctt 960 aaatgggaat tgatcggttt agaaatagac cttccttcgg gcaattttca aggtgctgca 1020 tggttgtcgt cagctcgtgc cgtgaggtgt taggttaagt cctgcaacga gcgcaacccc 1080 tgtcactagt tgccatcatt aagttgggga ctctagtgag actgcctacg caagtagaga 1140 ggaaggtggg gatgacgtca aatcatcacg gcccttacgc cttgggccac acacgtaata 1200 caatggccgg tacagagggc agctacacag cgatgtgatg caaatctcga aagccggtct 1260 cagttcggat tggagtctgc aactcgactc tatgaagctg gaatcgctag taatcgcgca 1320 tcagccatgg cgcggtgaat acgttcccgg gccttgtaca caccgcccgt caagccatgg 1380 aagtctgggg tacctgaagt cggtgaccgt aaaaggagct gcctagggta aaacaggtaa 1440 ctagggctaa gtcgtaa 1457 <210> 5 <211> 1443 <212> DNA <213> Spirosoma linguale HKMC-117 <220> <221> rRNA &Lt; 222 > (1) .. (1443) <223> 16s rRNA <400> 5 tggctcagga tgaacgctag cggcaggcct aatacatgca agtcgaacgg gccgcaaggt 60 cagtggcaaa cgggtgcgta acgcgtaagc aacctgccct caactggggg atagcccggc 120 gaaagctggg gtaaacccgc acggtcccac ggaatcacct ggttttttgg gtaaacattt 180 atgggttgag gaggggcttg cgtctgatta gctagttggc ggggtaacgg cccaccaagg 240 cgatgatcag taggggttct gagaggattg gcccccacat gggtactgag atacggaccc 300 aactcctacg ggaggcagca gtagggaata ttgggcaatg ggcgcaagcc tgacccagcc 360 atgccgcgtg caggatgaag gcgctcagcg ttgtaaactg cttttatcgg tgaagaacgg 420 cagtcctgcg ggattgtgtg acggtagccg aggaataagc accggctaac tccgtgccag 480 cagccgcggt aatacggagg gtgcaagcgt tgtccggatt tattgggttt aaagggtgcg 540 taggtggtcg tctaagtctg gttttaaagt tggctgctta acagtcaaag gtggctggaa 600 actggacgac ttgaatggga tggcggtagc cggaatgggt catgtagcgg tgaaatgcat 660 agatatgacc cggaacaccg attgcgaagg caggctacta cgtcccgatt gacactgagg 720 cacgagagca tgggtagcga acaggattag ataccctggt agtccatgcc gtaaacgatg 780 attactggct gtgtggtctt tgggctgcgt ggctgagcga aagcgttaag taatccacct 840 ggggagtacg ctggcaacag tgaaactcaa aggaattgac gggggtccgc acaagcggtg 900 gagcatgtgg tttaattcga tgatacgcga ggaaccttac ctgggctaga atgtgaagga 960 agttatcaga aatggtagcg tgtagcaata cacctgaaac aaggtgctgc atggctgtcg 1020 tcagctcgtg ccgtgaggtg ttgggttaag tcccgcaacg agcgcaaccc ctatgatttg 1080 ttgccagcat gtaatgatgg ggactcaagt cagactgcct gcgcaagcag agaggaagga 1140 ggggacgacg tcaagtcatc atggccctta cgcccagggc gacacacgtg ctacaatggt 1200 cggtacagcg ggtagcgaaa cggtaacgtt gagccaatct tgtaaagccg gtcacagttc 1260 cgcatcagcc 1320 atggcgcggt gaatacgttc ccggaccttg tacacaccgc ccgtcaagcc atgggagttg 1380 gggggacctg aagttcggtg tgacatccgg acaagggtaa actcggcgac tggggctaag 1440 tcg 1443 <210> 6 <211> 478 <212> DNA <213> Geobacillus toebii HKMC-118 <220> <221> rRNA &Lt; 222 > (1) .. (478) <223> 16s rRNA <400> 6 catggctcag gacgaacgct ggcggcgtgc ctaatacatg caagtcgagc ggaccgaacg 60 gaagcttgct tctgtttggt tagcggcgga cgggtgagta acacgtgggt aacctgcccg 120 taagaccggg ataactccgg gaaaccgggg ctaataccgg ataacaccga agaccgcatg 180 gtcttcggtt gaaaggtggc ttttgctacc acttacggat gggcccgcgg cgcattagct 240 agttggtgag gtaacggctc accaaggcga cgatgcgtag ccggcctgag agggtgaccg 300 gccacactgg gactgagaca cggcccagac tcctacggga ggcagcagta gggaatcttc 360 cgcaatggac gaaagtctga cggagcgacg ccgcgtgagc gaagaaggtc ttcggatcgt 420 aaagctctgt tgttagggaa gaagaagtac cgttcgaata gggcggtacg gtgacgta 478

Claims (17)

Geo Bacillus compost (Geobacillus toebii) is a screening method of the antimicrobial agent for reducing odor in the air conditioning apparatus for reducing the occurrence of odor in the air conditioner,
(a) preparing the above-mentioned Geobacillus compost or a culture thereof;
(b) contacting the sample to the Geobacillus compost or culture thereof;
(c) measuring inhibition of growth of the Geobacillus compost ; And
(d) determining that the sample has an antimicrobial activity to reduce the odor in the air conditioner generated by the Geobacillus compost when the growth of the Geobacillus compost is inhibited;
And a method for screening an odor-reducing antimicrobial agent in an air conditioner.
The screening method according to claim 1, wherein the air conditioner is an air conditioner.
According to claim 1, wherein said geo Bacillus compost is a screening method which comprises causing the odor to form a biofilm in the EVA core air conditioner.
4. The screening method according to claim 3, wherein the eva core is made of aluminum, aluminum alloy, copper or copper alloy.
The method of claim 1, wherein the Geobacillus compost is selected from the group consisting of Geobacillus toebii) HKMC-118 (Accession No: KCCM11694P) a screening method characterized in that the.
The method of claim 1 wherein the antimicrobial agent is a screening method characterized in that additionally holding the antimicrobial activity of the blood Brela air Stu arena (Fibrella aestuarina).
7. The method according to claim 6 , Air Stu arena is blood Brela air Stu arena (Fibrella aestuarina ) HKMC-115 (accession number: KCCM11691P).
The method of claim 1 wherein the antimicrobial agent is spiro Soma Ringgu Earl (Spirosoma linguale), and Spiro radio Soma tolerance (Spirosoma at least one selected from the group consisting of radiotolerans) spiro Soma Wherein the microorganism further has an antimicrobial activity against the genus Microorganism.
The method of claim 8, wherein the spiro Soma Microorganisms in Spiro Soma ringgu Earl (Spirosoma linguale) HKMC-117 (Accession No: KCCM11693P), and spiro Soma radio tolerance (Spirosoma radiotolerans) HKMC-114 (Accession No: KCCM11690P) a screening method characterized in that at least one selected from the group consisting of.
The method of claim 1, wherein the antimicrobial agent is selected from the group consisting of Chrysosobacterium A screening method characterized in that additionally holding the antimicrobial activity of the geo-car forces ferret (Chryseobacterium geocarposphaerae).
11. The method of claim 10, wherein Cri Seo tumefaciens Geocafos Ferre Is Cri Seo tumefaciens geo-car forces ferret (Chryseobacterium geocarposphaerae ) HKMC-116 (accession number: KCCM11692P).
The method of claim 1, wherein the antimicrobial agent is feromonas Furanyl Quebec (Pelomonas lt ; RTI ID = 0.0 & gt; puraquae. & lt ; / RTI & gt ;
13. The method of claim 12, wherein the ferromonus Furanyl Quebec is Perot Pseudomonas furanyl Quebec (Pelomonas puraquae) HKMC-113 (Accession No: KCCM11689P) a screening method characterized in that the.
delete delete The odor-causing microorganisms in compost Geo Bacillus (Geobacillus toebii) in the air conditioning system method within a deodorizing air conditioner, comprising the step of separating or death from the air conditioner.
Method in deodorizing the air conditioning apparatus, comprising the step of inhibiting the growth of odor-causing microorganisms in compost Geo Bacillus (Geobacillus toebii) in the air conditioner.

Images