KR101774560B1 - Plate-type chromatography device comprising chromatography column, detector and printer - Google Patents

Abstract

The present invention relates to a plate-type chromatography apparatus comprising a plate-type chromatography column, a detection section and an output section, which prevents a difference in flow rate between the central part and the side part of the column from occurring, and that one side or both sides of the distribution section and collecting section By providing a symmetrical inclined surface with respect to the vertical axis, it is possible to improve separation resolution of the chromatography by making the moving distance of the fluid constant. a pH sensor unit; And a conductivity sensor unit to detect the amount of control and the amount of the sample.

Description

[0001] The present invention relates to a plate-type chromatographic column, a plate-type chromatography column, a detector and an output unit,

The present invention relates to a plate-type chromatography apparatus comprising a plate-type chromatography column, a detection section and an output section, and more particularly to a plate-type chromatography apparatus comprising a sample inlet, a first frit section, a distributor for distributing the introduced fluid, A collecting portion, a second frit portion, and a discharge portion, and a detecting portion and an output portion connected to the column.

Chromatography was a technique developed for analysis in the early 1900s. A sample or a feed liquid containing a target substance to be separated or purified is injected into a chromatography column and sequentially eluted according to the interactions of the substances of various components contained in the sample with the chromatography column to efficiently separate or purify the target substance Technology. At present, various chromatographic techniques have been developed, and various chromatographic techniques can be selected and applied according to the characteristics of the substance to be analyzed or refined, and the configuration of equipment for implementing the selected technology is relatively easy, And analytical and manufacturing technologies that are widely used in universities and research institutes.

Batch chromatography, the most common chromatography technique, has a very simple configuration. A pump for injecting a sample to be separated or an injector, a column for separating a target material in a sample or a supply liquid, a pump for injecting a sample to be separated or a fluid to be eluted in the column, A detector or detector that detects the contained material, a collector that collects and collects the target material, and a connector (pipeline or tubing line) that connects each instrument.

It is also possible to separate and purify the target material with a very high efficiency by adding a temperature control jacket to maintain the temperature of the holder or column to protect the most important column in the chromatography apparatus, .

Although the technology of the chromatography apparatus having the above-described structure has been developed, there is a growing demand for separating a substance to be separated into a very small amount or a large amount of materials at a high resolution. In addition, there is a growing demand for separation and purification techniques for materials such as temperature sensitive proteins.

In this connection, as a known application technique of chromatography, there is a chromatographic column support for stabilizing the packing of a chromatography column disclosed in Korean Patent Laid-Open Publication No. 2014-0008340, and US Pat. No. 7575676 Pressure liquid chromatography jacket comprising a rectangular liquid-tight enclosure having a detachable cover in U. S. Patent No. 4,966, 695, discloses a high-pressure liquid chromatography jacket which is capable of separating trace amounts of material, A technique relating to a plate-type chromatography apparatus including a detection section and an output section capable of separating a substance at a high resolution in the separation process and capable of controlling, correcting and detecting temperature and pressure is not known.

SUMMARY OF THE INVENTION The present invention has been made in view of the above-mentioned needs, and it is an object of the present invention to provide a plate-type chromatography apparatus including a plate-shaped chromatography column, a detector and an output unit. More specifically, the moving distance and speed of a substance (fluid) to be separated or purified can be uniformly separated or purified without being affected by the length of the column, can be applied and controlled, and the temperature of the column The present invention has been accomplished on the basis of this finding by providing a plate chromatography apparatus capable of detecting and recording the amount and elution time of a substance to be separated.

In order to achieve the above object,

Plate chromatography column (C01); A detector D01 connected to the column; And an output unit (P01) connected to the detecting unit,

The plate-type chromatography column (C01) has a sample inlet (C001); A first frit portion C002 provided at a lower end of the injection port; A distributor (C003) disposed at a lower end of the first frit portion and distributing the fluid flowing through the first frit portion; A column body (C004) connected to the lower end of the distributor and separating the mixed components contained in the sample, the center of the column; A collector (C005) provided at a lower end of the column body; A second frit portion (C006) provided at the lower end of the column body portion; And an outlet (C007) provided at a lower end of the second frit portion are connected in sequence,

The detection unit D01 connected to the column includes a UV sensor unit D001; a pH sensor unit D002; And a conductivity sensor unit (D003).

The present invention relates to a plate-type chromatography apparatus comprising a plate-shaped chromatography column, a detection section and an output section.

The column of the present invention has a plate-like structure, and the different parts of the distributing part and the collecting part are formed with a constant slope so that they are not affected by the flow velocity or the length of the column, And includes a detection unit that can confirm the elution time or elution amount of a substance to be eluted conveniently. Therefore, it can be very useful for separating or purifying a large amount of sample from a very small amount of sample.

Fig. 1 is a schematic diagram showing a detection part D01 and an output part P01 included in the plate-type chromatography device of the present invention.
FIG. 2 is a plate-type chromatographic column (C01) according to an embodiment of the present invention, which is a model diagram in the form of a rectangular parallelepiped with a column body. C001 is the inlet of the sample; C002 denotes a first frit portion; C003 is a distribution part in which the injected fluid is distributed; C004 is the body part of the column; C005 is a collecting part for collecting the fluid passing through the column body part; C006 is a second frit portion; And C007 means an outlet for discharging the separated components.
FIG. 3 is a view showing a holder H01 that can be added to a plate-type chromatography apparatus according to an embodiment of the present invention and a support S01 included at a lower end of the holder.

Hereinafter, the present invention will be described in detail with reference to the drawings. It is to be understood by those skilled in the art that the drawings of the present invention are only for describing the present invention in more detail and that the scope of the present invention is not limited thereto.

The present invention relates to a plate chromatography column (C01); A detector D01 connected to the column; And an output unit (P01) connected to the detecting unit,

The plate-type chromatography column (C01) has a sample inlet (C001); A first frit portion C002 provided at a lower end of the injection port; A distributor (C003) disposed at a lower end of the first frit portion and distributing the fluid flowing through the first frit portion; A column body (C004) connected to the lower end of the distributor and separating the mixed components contained in the sample, the center of the column; A collector (C005) provided at a lower end of the column body; A second frit portion (C006) provided at the lower end of the column body portion; And a discharge port (C007) provided at a lower end of the second frit portion are sequentially connected to each other, and a detection portion (D01) connected to the column includes a UV sensor portion (D001); a pH sensor unit D002; And a conductivity sensor unit (D003).

As shown in FIG. 1, the present invention includes a detection unit D01 and an output unit P01. The UV sensor unit D001 included in the detection unit senses a material to be eluted in the column, Is preferably in the range of 190 nm to 800 nm.

In the apparatus of the present invention, pressurization applies a pressure in the column by connecting a pump to the column. The pump is selected from among a pressurizing pump, a circulating pump, a vacuum pump, a submerged pump, a rotary pump and an automatic pump And more preferably a rotary (rotary) pump.

The pH sensor unit D002 preferably irradiates light that does not affect the sample and uses the transmittance of light capable of measuring and analyzing the amount of light transmitted through the optical element, but is not limited thereto.

The conductivity sensor unit D003 is a reciprocal value of electrical resistance, and uses Siemens (S) units to sense a value obtained by dividing a current in a column by a voltage.

In addition, since the detecting unit P01 of the present invention has a 'log memory', parameters such as experimental conditions and result values can be recorded, and the recorded value can be stored in the detecting unit. The output P01 may include input parameters and operating software as well as output and storage of results. It is also possible to further mount application software for processing or interpreting the obtained results.

As shown in FIG. 2, the sample inlet C001 of the column C01 is a portion for directly injecting a sample or injecting a sample by connecting it with a pipe or a tubing line; The first frit portion C002 serves to remove insoluble impurities that may be contained in the fluid introduced into the plate-type chromatography column. The first frit portion may be in the form of a mesh or a filter, but the present invention is not limited thereto and can be appropriately changed as necessary.

Also, the distributor C003 means from the lower end of the first frit portion to just before the body portion of the column. The sample inlet and the fluid flowing into the first frit portion are distributed, and one side or both sides are inclined at a predetermined slope.

The filler to be filled in the plate-type chromatography column of the present invention can be appropriately filled depending on the purpose, so that the filler is not particularly limited, but preferably is an ion exchange resin, a size exclusion resin, A resin selected from the group consisting of an affinity chromatography resin, a reverse phase and a normal phase chromatography resin, a chiral stationary phase resin, aluminum oxide, activated carbon, magnesium oxide, starch, cellulose, It is one.

In addition, the column body C004 is a portion where separation of various components contained in the sample is performed, and the same ion exchange resin, size exclusion resin, affinity chromatography chromatography (affinity chromatography) A chromatographic column filler such as a resin, a reverse phase and a normal phase chromatography resin, a chiral stationary phase resin, aluminum oxide, activated carbon, magnesium oxide, starch, cellulose, And can be suitably charged accordingly.

In addition, the column body C004 may be a rectangular parallelepiped type or an elliptical type having a curved surface. In the case of a thin plate-like structure as a whole, its shape and shape are not excluded. That is, it is characterized by a plate-like structure capable of minimizing a difference in flow velocity between the center portion and the side portion. In the column structure of the present invention, the circular structure has a separation resolution due to a difference in flow rate between the center portion and the side portion, so that the circular structure is excluded. Preferably, the column body C004 has a length of 4.6 mm to 2.0 m, a length of 5.0 mm to 5.0 m, and a thickness of 1.5 mm to 1.0 m, but the present invention is not limited thereto.

The collecting part C005 is a part where the fluid passing through the column body C004 is collected and is inclined at one side or both sides with a slope which is symmetrical with the inclined surface of the distributing part C003 about the vertical axis . 3, the column of the present invention is characterized in that efficient separation and purification are performed by minimizing the difference in flow velocity according to the flow path of the fluid.

In addition, the second frit portion C006, like the first frit portion, functions to remove unnecessary impurities. The second frit portion C006 may have the same mesh shape or filter shape as the first frit portion.

The outlet (C007) is the lower end of the chromatography column, where it separates or purifies the separated material. It can also capture the directly separated or purified material, or it can be connected to a detection system with a connection tube to detect or analyze quantitatively It is possible.

The plate-type chromatography apparatus of the present invention can add a connection pipe (pipe or tubing line) capable of interlocking a detection device or a detector capable of quantitatively confirming the amount of separated materials or elution time, .

In addition, the column of the present invention is preferably manufactured as an integral type, but it is not excluded that the separator and the body, the body and the collector are separately manufactured. If the separator is manufactured separately, It is characterized by the connection without leakage of fluid.

Further, as disclosed in FIG. 3, the present invention may further include a holder that further serves as a support for the column. The holder not only serves as a support for the plate-type chromatography column but also can include a pipeline (tube) capable of preventing the outer shape of the column from being deformed by pressure and allowing the fluid to flow in and out for temperature control And a support S01 at a lower portion of the holder.

Characterized in that the inner portion of the support and the holder is pierced so that the outlet of the column mounted in the holder can be positioned on the underside of the support.

The holder may further include an inlet, a discharge port, and a built-in pipeline (tube) for the inflow and discharge of the temperature controlling fluid, and may be added as necessary.

The pipe for controlling the temperature may be included on one side or both sides of the holder, and water or nitrogen gas may be used as the fluid for temperature control, but the present invention is not limited thereto.

Further, the column can be used irrespective of whether it is a column that can be used permanently or semi-permanently or a disposable column.

C01: Column
C001: Sample inlet C002: First frit portion
C003: distribution part C004: column body part
C005: collecting part C006: second frit part
C007:
D01:
D001: UV sensor part D002: pH sensor part
D003: Conductivity sensor part D004: Correction sensor part
P01: Output section
H01: Holder
H001: Inlet H002: Outlet
S01: Support

Claims (5)

Plate chromatography column; A detector coupled to the column; And an output unit connected to the detection unit,
The plate-type chromatography column has an inlet of a sample; A first frit portion provided at a lower end of the injection port; A distribution unit disposed at a lower end of the first frit unit and distributing the fluid flowing through the first frit unit; A column body connected to the lower end of the distributor and separating mixed components contained in the sample, the center of the column; A collector disposed at a lower end of the column body; A second frit portion provided at a lower end of the column body portion; And a discharge port provided at a lower end of the second frit portion are sequentially connected to each other, and the detection portion connected to the column includes a UV sensor portion, a pH sensor unit; Wherein the collecting portion comprises a sloped surface having a slope that is symmetrical with respect to an inclined surface of the distributing portion about a vertical axis, the inclined surface having one side inclined at a predetermined inclination, Wherein lengths and angles of both sides of the first frit portion are equal to the lengths and angles of both sides of the second frit portion around the discharge port. The apparatus of claim 1, wherein the apparatus further comprises a pump. The apparatus of claim 1, wherein the output unit is a unit for recording or storing the flow rate, pressure, and temperature to be controlled by the detection unit. The apparatus of claim 1, wherein the column is disposable. 5. The plate chromatography apparatus according to any one of claims 1 to 4, wherein the plate chromatography apparatus further comprises a holder.

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