KR101773467B1 - A Valve actuator for DNA Extracting Systems - Google Patents

A Valve actuator for DNA Extracting Systems Download PDF

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Publication number
KR101773467B1
KR101773467B1 KR1020150150656A KR20150150656A KR101773467B1 KR 101773467 B1 KR101773467 B1 KR 101773467B1 KR 1020150150656 A KR1020150150656 A KR 1020150150656A KR 20150150656 A KR20150150656 A KR 20150150656A KR 101773467 B1 KR101773467 B1 KR 101773467B1
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KR
South Korea
Prior art keywords
driving
valve
drive
drive shaft
key
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KR1020150150656A
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Korean (ko)
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KR20170051562A (en
Inventor
박병윤
박상덕
조정산
백승호
이성호
Original Assignee
한국생산기술연구원
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Priority to KR1020150150656A priority Critical patent/KR101773467B1/en
Publication of KR20170051562A publication Critical patent/KR20170051562A/en
Application granted granted Critical
Publication of KR101773467B1 publication Critical patent/KR101773467B1/en

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    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K31/00—Actuating devices; Operating means; Releasing devices
    • F16K31/02—Actuating devices; Operating means; Releasing devices electric; magnetic
    • F16K31/04—Actuating devices; Operating means; Releasing devices electric; magnetic using a motor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K3/00—Gate valves or sliding valves, i.e. cut-off apparatus with closing members having a sliding movement along the seat for opening and closing
    • F16K3/22—Gate valves or sliding valves, i.e. cut-off apparatus with closing members having a sliding movement along the seat for opening and closing with sealing faces shaped as surfaces of solids of revolution
    • F16K3/24—Gate valves or sliding valves, i.e. cut-off apparatus with closing members having a sliding movement along the seat for opening and closing with sealing faces shaped as surfaces of solids of revolution with cylindrical valve members
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K31/00—Actuating devices; Operating means; Releasing devices
    • F16K31/02—Actuating devices; Operating means; Releasing devices electric; magnetic
    • F16K31/04—Actuating devices; Operating means; Releasing devices electric; magnetic using a motor
    • F16K31/047—Actuating devices; Operating means; Releasing devices electric; magnetic using a motor characterised by mechanical means between the motor and the valve, e.g. lost motion means reducing backlash, clutches, brakes or return means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1002—Reagent dispensers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
    • G01N35/1016—Control of the volume dispensed or introduced
    • G01N2035/102—Preventing or detecting loss of fluid by dripping
    • G01N2035/1023—Preventing or detecting loss of fluid by dripping using a valve in the tip or nozzle
    • G01N2035/1067—

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  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Mechanical Engineering (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention relates to a valve driver for a DNA extraction system, and more particularly, to a valve driver capable of sequentially opening and closing a plurality of valves with a single power source. A drive shaft for opening / closing each valve; a drive shaft for driving the drive shaft; a drive shaft for driving the drive shaft; a drive shaft for driving the drive shaft; And a drive unit including a drive key having one end connected to the inside of the housing and a drive key elastically supported so as to be able to be retracted and expanded by a spring, wherein the power source is formed as one unit, And a driven pulley, a driven pulley, and a timing belt. The drive shaft of the drive means is smoothly and easily moved to one side of the driven gear, the driven gear, the driven pulley, and the driven pulley. And a support bracket is formed at intervals.

Description

[0001] The present invention relates to a valve actuator for a D & A extraction system,

The present invention relates to a valve driver for a DNA extraction system, and more particularly, to a valve driver capable of sequentially opening and closing a plurality of valves with a single power source.

Generally, in order to diagnose, treat, or prevent disease at the genetic level, a biological substance or DNA (deoxyribonucleic acid: a nucleic acid having two types of nucleic acids present in nature and having deoxyribose, from a biological sample such as a cell, A DNA extraction system is used for extracting or amplifying DNA.

Such a DNA extraction system can be used in a DNA extraction process, such as a pipette (Pipette: measuring a liquid or gas of a constant volume, or a device that can be added to another container) or a DNA pump A plurality of valves for moving a liquid such as a drug, a fluorescent substance, and a washing solution to another container, and a plurality of drivers for driving the respective valves.

However, since the conventional valve driver for the DNA extraction system is provided with an independent actuator for rotationally driving each valve, the manufacturing cost, weight and size are increased, the structure is complicated, and the liquid is moved to another container There is a problem that it is difficult to control them sequentially.

If the position of the initial valve is wrong, the key groove does not exactly coincide with the drive key, so that smooth coupling can not be achieved, so that the operation is delayed or the operation is very cumbersome and takes a long time.

Korean Patent Publication No. 2002-0085123

SUMMARY OF THE INVENTION The present invention has been made in order to solve the above problems, and it is an object of the present invention to reduce the weight and size of a valve by sequentially opening and closing a plurality of valves with a single power source, The present invention also provides a valve driver for a DNA extraction system, which improves workability by a smooth coupling with a key.

According to an aspect of the present invention, there is provided an electric power steering apparatus including a power source for providing a motor power, a connecting means provided for interlocking with the power source to transmit rotational power, And a drive unit comprising a housing coupled to the drive shaft and the drive shaft, and a drive unit coupled to one side of the housing and elastically supported by a spring so as to be elastically stretchable, the drive unit comprising: And the connecting means is formed of a driving gear and a follower gear, or is formed of a driving pulley, a driven pulley and a timing belt, and at one side of the driving gear, the driven pulley and the driven pulley, A support bracket is formed at an interval so that movement is smooth and easy And that is characterized.

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As described above, according to the present invention, a plurality of valves are sequentially opened and closed by a single power source, thereby forming a simple structure, reducing weight and size, and reducing cost.

Further, even if the initial position of the valve is wrong, the present invention can smoothly and easily engage with the drive key, thereby improving the workability.

1 is a partially exploded view of a valve driver for a DNA extraction system according to a preferred embodiment of the present invention.
Fig. 2 is a view showing the coupling state of Fig. 1. Fig.
Fig. 3 is a sectional view showing the main portion of a valve actuator for a DNA extraction system according to the present invention.
4 is a schematic view showing a state in which a valve driver according to the present invention is installed in a DNA extraction system.
5 is an enlarged view of a valve according to the present invention.
6 is an assembled view of Fig.
Fig. 7 is a schematic view showing the longitudinal section of Fig. 6. Fig.
8 is a view schematically showing the cross-sectional state of Fig.
9 is a view showing another embodiment of the connecting means according to the present invention.

Hereinafter, preferred embodiments of a valve actuator for a DNA extraction system according to the present invention will be described in more detail with reference to the accompanying drawings.

Hereinafter, elements having the same function in all the following drawings will be denoted by the same reference numerals, and repetitive description will be omitted. Further, the following terms are defined in consideration of functions in the present invention, Should be interpreted as.

As shown in FIGS. 1 to 8, the present invention is generally divided into a power source 110, a connecting means 120, and a driving means 130.

The power source 110 is an electric motor that converts electrical energy into mechanical energy to provide rotational power.

Here, the electric motor may be a step motor (a motor rotating at a certain angle by a pulse-like voltage) or a servomotor (a motor system capable of controlling the speed with its own speed control) However, the present invention is not limited to this, and various types of motors may be used as long as the rotational speed can be precisely controlled.

The connecting means 120 is connected to the power source 110 and is connected to both ends of the driving gear 121 for transmitting rotational power to the driving means 130, And a driven gear 122 for transmitting rotational power to the driving means 130. [

The driving gear 121 and the driven gear 122 are supported by a support bracket 121a so that the driving shaft 131 of the driving unit 130 to be described later is smoothly and easily moved forward and backward, , And 122a.

That is, the support brackets 121a and 122a are formed on the respective sides of the driving gear 121 and the driven gear 122 with a gap therebetween, and the driving shaft 131 is driven through the support brackets 121a and 122a , So that it is possible to move backward.

The key tip 221 of the valve 220 and the key tip 134a of the drive key 134 constituting the drive means 130 are different from each other so that when the drive key 134 is replaced, Can be moved to one side to achieve a smooth replacement operation.

It is preferable that the driven gear 121 and the driven gear 122 rotate in the same direction.

For example, when the driving gear 121 rotates in the clockwise direction, it is preferable that the driven gears 122, which are engaged with each other and interlocked with each other, also rotate clockwise.

On the contrary, when the driving gear 121 rotates counterclockwise, it is preferable that each of the driven gears 122 coupled to the driven gear 121 rotates counterclockwise.

However, the rotation angle of the valve 220, which is opened and closed in conjunction with the driven gear 122, may also be configured to be opposite to that of the driven gear 122 121 which are opened and closed in cooperation with the valve 220.

When the driving gear 130 rotates in the same direction as the driven gear 121 and the driven gear 122 rotate in the same direction as described above, A liquid such as a substance, a washing solution may be sequentially injected into the valve 220 having the liquid outflow inlet 222 and transferred to the other container through the transfer pipe 240.

The driving means 130 is provided to be linked to the rotation operation of the connecting means 120 so as to be coupled to the driving shaft 131 and the driving shaft 131 in order to open and close the liquid outlet opening 222 of each valve 220. [ And a drive key 134 which is coupled to one side of the housing 132 and is elastically supported by the spring 133 so as to be stretchable.

One end of the drive shaft 131 is installed to be able to move forward and backward through the central portions of the support brackets 121a and 122a of the driven gear 121 and the driven gear 122, And is rotationally operated in conjunction with the rotational force of the driving gear 121 and the driven gear 122.

Accordingly, when the drive key 134 is replaced, the drive shaft 131 can be easily moved forward and backward to achieve a smooth replacement operation.

In addition, the housing 132 has a coupling groove 132a for receiving and coupling one side of the drive shaft 131, and a long groove 132b is formed on both sides along the longitudinal direction.

The driving key 134 is formed with a coupling protrusion 134a on the outer peripheral surface of the rear end portion to be coupled with the housing 132 to support a proper and smooth expansion and contraction operation, A key tip 134b for engagement with the key 221 is formed.

When the valve 220 is mounted on the cartridge 210 of the DNA extraction system 200, the driving means 130 having such a configuration rotates in conjunction with the connecting means 120 interlocked with driving of the power source 110, 220, respectively.

At this time, if the key tip 134b of the driving means 130 does not coincide with the key groove 221 of the valve 220, the driving key 134 elastically supported by the spring 133 is pushed back, When the tip 134b rotates continuously and coincides with the key groove 221, the tip 134b advances by the resilient restoring force of the spring 133 and is engaged to rotate the valve 220. [

The operation of the valve driver according to the present invention will now be described.

First, a DNA extracting system 200 equipped with a valve driver 100 according to the present invention is used to diagnose, treat or prevent a disease at a genetic level. In order to diagnose, treat or prevent a disease, biological substances or DNA a plurality of valves 220 that can open and close the passageways for passing, blocking, or controlling a liquid such as a drug, a fluorescent material, and a washing solution in the cartridge 210 during the extraction or amplification of the deoxyribonucleic acid, And the outflow inlet 222 are arranged at different angles.

Next, the power source 110 of the valve driver 100 installed to correspond to the key groove 221 of each valve 220 is operated.

When the power source 110 is rotated, the driving gear 121 and the driven gear 122 of the connecting means 120 are rotated in conjunction with each other so that the driving shaft 131 of the driving means 130 The housing 132 and the drive key 134 are coupled to the valve 220 while rotating.

That is, the drive key 134 is moved to the key groove 221 of the valve 220 while the key tip 134b rotates in a direction in which the valve 220 is closed so as to be completely engaged at the initial position of the valve 220 .

When the key tip 134b of the drive key 134 and the key groove 221 of the valve 220 are not aligned with each other, the drive key 134 elastically supported by the spring 133 is pushed backward And the key tip 134b continues to rotate. When the key tip 134b coincides with the key groove 221, the compressed spring 133 returns, and the drive key 134 is pushed to complete the engagement.

Subsequently, the drive keys 134 coupled to the respective valves 220 are rotated to sequentially rotate the liquid outlet ports 222 of the respective valves 220.

That is, since each of the valves 220 is disposed so as to form different angles with respect to the liquid outlet port 222 at the initial setting, the inlet port 222 of the liquid transfer pump 230 of the DNA extraction system 200, As shown in FIG.

The liquid transfer pump 230 can inject liquids such as chemicals, fluorescent materials, and washing solutions into the respective valves 220 only when the injection ports coincide with the liquid outlet ports 222 of the respective valves 220 .

Since this control method is generally used, a detailed description will be omitted.

Next, the liquid injected by the liquid transfer pump 230 passes through the valve 220 and is transferred to another container along the transfer pipe 240.

Accordingly, the valve driver for the DNA extraction system according to the present invention continuously repeats the above-mentioned operation to sequentially open and close a plurality of valves by one driving means, thereby forming a simple structure, reducing weight and size, You can.

Further, according to the present invention, even if the initial position of the valve is wrong, the coupling with the actuator can be smoothly and easily performed, and workability can be improved.

9 is a view showing another embodiment of the connecting means according to the present invention in that the connecting means 120 is formed by a driving pulley 123, a plurality of driven pulleys 124 and a timing belt 125 Since the rotational power transmitted from one power source 110 is smoothly and easily transmitted to the plurality of driving means 130, it is possible to simplify the configuration, thereby reducing the weight and size, as well as improving the power transmitting ability.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the invention. And will be apparent to those skilled in the art to which the invention pertains.

100: valve actuator 110: power source
120: connecting means 121: driving gear
121a, 122a: support bracket 122: driven gear
123: idler pulley 124: idler pulley
125: timing belt 130: driving means
131: drive shaft 132: housing
132a: coupling groove 132b: long groove
133: spring 134: drive key
134a: engaging projection 134b: key tip
200: DNA extraction system 210: cartridge
220: Valve 221: Key groove
222: liquid outflow inlet 230: pump
240: transfer pipe

Claims (5)

delete delete delete delete A driving shaft installed to be interlocked with the power source to transmit rotational power, a driving shaft installed to be interlocked with a rotating operation of the connecting means, for opening and closing each valve, and a housing coupled to the driving shaft; And a drive unit including a drive key having one side coupled to the inside of the housing and elastically supported to be able to be stretched and contracted by a spring, the valve driver comprising:
The power source is formed as one, and the connecting means is formed of a driving gear and a follower gear, or is formed of a driving pulley, a driven pulley and a timing belt, and the driving gear, the driven pulley, And a support bracket is formed at an interval so as to smoothly and easily move the drive shaft forward and backward.
KR1020150150656A 2015-10-29 2015-10-29 A Valve actuator for DNA Extracting Systems KR101773467B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020150150656A KR101773467B1 (en) 2015-10-29 2015-10-29 A Valve actuator for DNA Extracting Systems

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020150150656A KR101773467B1 (en) 2015-10-29 2015-10-29 A Valve actuator for DNA Extracting Systems

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KR20170051562A KR20170051562A (en) 2017-05-12
KR101773467B1 true KR101773467B1 (en) 2017-09-13

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Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102107548B1 (en) * 2019-08-08 2020-05-07 주식회사 엘지화학 Device for processing fluid of diagnosing
KR102568221B1 (en) * 2021-12-06 2023-08-21 한국원자력연구원 Rotary apparatus for containing multi sample

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR200309154Y1 (en) * 2002-11-06 2003-03-31 오용수 Electromotion three way ball valve
JP2005241133A (en) * 2004-02-26 2005-09-08 Trinity Ind Corp Automatic damper device

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR200309154Y1 (en) * 2002-11-06 2003-03-31 오용수 Electromotion three way ball valve
JP2005241133A (en) * 2004-02-26 2005-09-08 Trinity Ind Corp Automatic damper device

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