KR101757734B1 - Cosmetic composition for promoting the stemness of adipose-derived stem cell and proliferating skin cell containing sequoia callus cell extracts - Google Patents

Abstract

The present invention provides a composition for promoting stem cell growth and a composition for skin cell proliferation of an adipose-derived stem cell comprising an extract of Sequoia callus as an active ingredient. The composition containing the extract of the present invention as an active ingredient promotes the regeneration of adipose stem cells and promotes the proliferation of metastasis-amplified cells of the skin epidermal basal layer involved in regeneration of the epidermis, thereby enhancing skin volume, It is possible to apply the composition for skin aesthetic and molding for increasing skin volume and the composition for external application for skin for increasing skin volume.

Description

TECHNICAL FIELD [0001] The present invention relates to a composition for promoting stem cell growth and skin cell proliferation of adipose-derived stem cells, which contains extract of Sequoia callus, and a composition for skin cell proliferation,

The present invention relates to a composition for promoting stem cell growth and skin cell proliferation of an adipose derived stem cell containing a succulent callus extract.

The skin is the largest organ of the human body, accounting for about 16% of our body volume, 1.8 m ^{2 in} area, 2 ~ 4 mm in thickness, weighing about 3 kg and composed of epidermis, dermis and subcutaneous fat layer . Such skin is in direct contact with the external environment and plays an important protective layer for protecting the human body from various harmful factors such as harmful microorganisms and ultraviolet rays, and at the same time, it has a biochemical function necessary for metabolism of the whole body. It is an indispensable institution. In particular, the subcutaneous fat layer below the dermis is composed of adipocyte lobules. It serves as a cushion for absorbing the protection of internal organs of the skin against external heat, and the nutrient component is formed in the form of triglyceride (Lipid droplet), which is stored in the organelle and used as an energy source if necessary. However, as the age increases, the skin becomes damaged due to natural factors and various external stimuli. In particular, the activity and expression of collagen (collagen) and elastic fiber (elastin) At the same time, the fat production in the subcutaneous fat is reduced, so that the original function of each constituent cell in the skin is not achieved properly. Therefore, the widely used method is fat graft surgery, which uses a syringe with a tulip needle-shaped tip to remove its own fat and transplant it to each part of the skin that is not elastic It is an operation. However, it has been reported that 40-60% of the transplant volume is resorbed into the human body after the fat transplant operation, and fat grafting at the site where the skin is thin and the subcutaneous fat is low causes problems such as cell mass being touched , The survival rate of the transplanted fat and the survival period are less than expected and re-transplantation is necessary.

Accordingly, there is an increasing demand for a composition capable of sufficiently increasing the subcutaneous fat layer while replacing the fat transplant surgery with these problems. Recently, retinoids such as retinol and retinoic acid, which have been developed and used for the purpose of inhibiting the reduction of collagen and elastin, cause skin elasticity reduction, exhibit excellent skin elasticity improvement effect. However, . In recent years, there has been a growing demand for the development of cosmetics using safer and less irritating ingredients in order to solve the skin irritation problem of such raw materials.

Since the basic structure of the skin is maintained by subcutaneous fat tissue, and this subcutaneous fat tissue plays a role in the volume sense and strength of the skin, increasing the volume of the adipose tissue may be a good solution for maintaining the volume of the skin , It is necessary to develop a composition capable of increasing the volume of adipose tissue without skin irritation in order to maintain and enhance the volume of the skin.

On the other hand, keratinocyte stem cells (KSC) of the skin present in the basal layer are known to regenerate in the epidermis located at the outermost part of the skin. The keratinocyte stem cells continue to divide and produce two large cells: the same stem cells that are made by self-renewal, and the other is Transit-Amplifying Cells (TA cells) . Metastasis-amplified cells are further amplified and differentiated early to become keratinocyte cells, which continue to differentiate and push them up to the surface of the skin. In this process, the keratinocytes are differentiated and keratinized, forming the stratum corneum, and after 3 ~ 4 weeks they are separated from the stratum corneum.

The keratinocyte and metastasis-amplified cells are usually distributed in an extremely small number of about 4-7% of the basal layer cells, and increasing the number of keratinocyte cells is not as easy as increasing the number of metastasis-amplifying cells, And it is not distributed in a wider area. Therefore, it was necessary to develop a composition showing the proliferative capacity of keratinocyte stem cells and metastasis-amplified cells.

KR 10-2009-0092235 A

In order to solve the above-mentioned problems, it is an object of the present invention to provide a composition containing a succulent callus extract which is capable of increasing adipose tissue without irritation to skin. It is another object of the present invention to provide a composition for promoting stem cell function of adipose derived stem cells and for increasing skin volume, as well as proliferating metaplasia cells of keratinocytes and regenerating skin cells.

In order to accomplish the above object, an embodiment of the present invention can provide a composition for promoting stem cell growth of an adipose derived stem cell comprising an extract of Sequoia callus as an active ingredient.

One embodiment of the present invention can provide a composition for skin cell proliferation comprising an extract of Sequoia callus as an active ingredient.

One embodiment of the present invention can provide a skin cosmetic and molding composition for enhancing skin volume retention which contains the extract of Calli extract as an active ingredient.

One embodiment of the present invention can provide a composition for external application for skin for increasing skin volume, which contains an extract of Sequoia callus as an active ingredient.

An embodiment of the present invention can provide a skin cosmetic method by promoting skin cell proliferation, which comprises treating the skin with a succulent callus extract.

The composition containing the extract of the present invention as an active ingredient promotes the regeneration of adipose stem cells and promotes the proliferation of metastasis-amplified cells of the skin epidermal basal layer involved in regeneration of the epidermis, thereby enhancing skin volume, The composition of the present invention can be applied to cosmetic compositions for increasing skin volume, composition for molding, and compositions for external application for skin volume increase.

1 is a graph showing cytotoxicity of adipose-derived stem cells according to concentration of a composition according to an embodiment of the present invention.
2 is a graph showing cytotoxicity of keratinocytes according to the concentration of the composition according to an embodiment of the present invention.
FIG. 3 is a graph showing expression patterns of CD29 and CD44 genes when oxidative stressed adipose stem cells were treated with a composition according to an embodiment of the present invention.
FIG. 4 is a graph showing the expression pattern of the CD71 gene when the composition according to an embodiment of the present invention is treated with ultraviolet irradiated keratinocytes.
FIG. 5 is a graph comparing the expression of (a) CD29, (b) CD44 protein when the composition according to an embodiment of the present invention is treated with oxidative stress-induced adipose stem cells.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings, so that those skilled in the art can easily carry out the present invention.

The present invention provides a composition for enhancing stem cell health of an adipose-derived stem cell comprising an extract of Sequoia callus as an active ingredient.

Sequoia is a tree of the Quercus variabilis, the only species belonging to the Sequoia. It grows in North America, Western California. The name "Sequoiere" also refers to another species belonging to the Sequoiatic family, the Sequoihedron (giant sequoia) or a metasequoia.

Callus (or callus, or union tissue, wound tissue, or oily tissue) is an undifferentiated irregular cell mass, and is a typical tumor tissue formed by a cleavage tissue around a wound when a plant is injured. Plants are composed of dividing tissues that are largely dividing cells and permanent tissues. When cells of the first dividing line are put in nutrient medium, callus is formed. Thereafter, males are formed and differentiated into plants. Callus is often called 'plant stem cells'.

The calli extract of Sequoia callus extracts callus from the leaves of the sequoia and then solid cell culture to establish a stem cell line and a large number of active ingredients in a suspension cell culture It is produced and extracted. The method for extracting the active ingredient from the calli can be extracted by a plant culture method of a cell line derived from the tissue explant of the plant as described in Korean Patent Publication No. 2007-0113193, A stable plant cell line derived from a Sequoia can be extracted by eluting with a mixture of an alcohol of 5-carbon atoms or less. However, the extraction method is not limited to the above-mentioned extraction method.

The succulent callus extract may be purchased and used commercially. For example, it can be purchased from Bio-FD & C of Korea.

The term 'stemness' refers to a state of stem cells having the self-renewal ability and homeostasis of stem cells. The term 'stem cell growth' refers to an increase in stem cell activity.

The adipose derived stem cell (ADSC) may be human adipose derived stem cells (hADSCs), but is not limited thereto.

The composition for promoting stem cell proliferation of the adipose-derived stem cells includes the expression of the differentiation cluster 29, integrin beta 1 (hereinafter referred to as CD29) and the differentiation cluster 44 (hereinafter referred to as CD44), which are adipose-derived stem cell markers Can be increased. The increased expression of CD29 and CD44 means that the activity of adipose derived stem cells is increased. Therefore, the composition for promoting stem cell growth of the adipose derived stem cells may have an effect of increasing the volume of the skin by increasing the adipose tissue.

The composition for enhancing stem cell characteristics of the adipose derived stem cells may contain 0.0001 to 10% by weight, and more specifically 0.0001 to 0.01% by weight, of the Sequoia callus extract, based on the total weight of the composition. If the amount is less than 0.0001% by weight, the effect is insignificant. If the amount is 10% by weight or more, there is a problem that the composition exhibits cytotoxicity.

Also, an embodiment of the present invention provides a composition for skin cell proliferation comprising an extract of Sequoia callus as an active ingredient.

The skin cell may be a transit-amplifying cell (TA cell), but is not limited thereto.

According to the composition for skin cell proliferation, the skin epidermis can be regenerated by promoting the proliferation of metastasis-amplified cells, and the metastasis-amplifying cells together with the keratinocyte cells can cause epidermal loss due to the elimination of keratinocytes through continuous cell division Overcome, and can play a role in regenerating the epidermis. Therefore, the composition for skin cell proliferation can regenerate the skin or prevent skin aging.

As used herein, the term " skin regeneration " is a broad concept including the ability to generate new keratinocytes in response to an overall skin aging, a wound caused by skin epidermis, and the like. Specifically, skin regeneration further amplifies the metastasis-amplifying cells generated by the division of keratinocyte stem cells existing in the basal layer of skin epidermis, and early differentiation becomes keratinocyte, which is pushed up to the surface of the skin, (Keratinization) and the formation of the stratum corneum means all the process. Among them, the key process of skin regeneration is the proliferation of keratinocyte stem cells in the basal layer and metastasis - amplification cells which are daughter cells of the cells. It is present in the basal layer of the skin in order to restore the skin damage caused by external physical and chemical environment such as abrasion which is only the outer surface of the skin, heat which appears when it is scratched, Active replication of keratinocyte stem cells and metastasis-amplifying cells responsible for regeneration is necessary. The reason for the scar remains when the skin is damaged due to age, while the scar is not left even when the skin damage occurs at a normal age. The level of proliferation of keratinocyte and metastasis-amplification cells necessary for skin regeneration is remarkable depending on the age . That is, as the aging progresses, the degree of proliferation of these cells becomes low, which leads to a decrease in skin regeneration ability, which may delay the recovery of wounds and the like and reduce skin elasticity.

The skin cell proliferated using the composition for skin cell proliferation can be transplanted into an individual in need of keratinocyte stem cells.

As demonstrated by the following test examples, the inventors of the present invention applied a skin cell proliferation composition containing an extract of Sequoia callus extract according to the present invention as an active ingredient to dermal keratinocytes, and found that the expression of metastasis-amplified cell markers Effective increase.

 As mentioned above, the keratinocyte cells play an important role in regenerating the epidermis. Therefore, through the increase of the number of keratinocyte stem cells by the composition according to the present invention, it is possible to improve skin regeneration ability for wound healing and to prevent skin elasticity and skin aging from deepening due to decline in skin regeneration ability.

The composition may increase the expression of the marker of the metastasis-amplification cell expressing Cluster of Differentiation 71 (hereinafter referred to as CD71), and CD71 is a marker marker protein specific for metastasis-amplified cells.

The CD71 protein, also referred to as the transferrin receptor, is present only in the basal layer. Specifically, the CD71 protein interacts with a transferring protein that binds to iron flowing in the cell blood stream, and acts as a receptor mediated transferrin endocytosis to supply iron in the cells do. Activated or proliferating cells require a large amount of iron in the cells, which can increase the expression of the CD71 protein.

The composition for skin cell proliferation may contain 0.0001 to 10% by weight, and more specifically 0.0001 to 0.01% by weight of Sequoia callus extract, based on the total weight of the composition. If the amount is less than 0.0001% by weight, the effect is insignificant. If the amount is 10% by weight or more, there is a problem that the composition exhibits cytotoxicity.

In addition, one embodiment of the present invention provides a skin cosmetic and molding composition for enhancing skin volume retention comprising an extract of Sequoia callus as an active ingredient.

The composition may be used, for example, for the purpose of removing wrinkles and wrinkles and maintaining elasticity by enhancing the volume feeling on the face such as breast enlargement through breast tissue formation, micro fat grafting, autologous fat transplantation.

The composition may be formulated into a unit dosage form suitable for administration to a patient in the body according to conventional methods in the pharmaceutical field. As a formulation suitable for such purpose, an injectable preparation or a preparation for topical administration is preferably used as the parenteral administration preparation. The injectable formulations are preferably isotonic aqueous solutions or suspensions. However, at this time, diluents or excipients such as commonly used fillers, extenders, binders, wetting agents, disintegrants, surfactants and the like can be used together.

The thus prepared preparation of the present invention can be administered parenterally according to a conventional method, for example, directly to a specific site. At this time, the composition is injected using a syringe. The daily dose of the composition is 1 × 10 ⁵ to 1 × 10 ⁷ cells / kg body weight, preferably 5 × 10 ⁵ to 5 × 10 ⁶ cells / kg body weight, and can be administered once or several times . It should be understood, however, that the actual dosage of the active ingredient should be determined in light of various relevant factors such as the amount of fat cells to differentiate and proliferate, the route of administration, the weight of the patient, age and sex, And are not intended to limit the scope of the invention in any way.

In addition, one embodiment of the present invention provides a composition for external application for skin for enhancing skin volume, which comprises an extract of Sequoia callus as an active ingredient.

The composition for external application for skin may be a cosmetic composition or a pharmaceutical composition.

The formulation of the cosmetic composition is not particularly limited and may be appropriately selected according to the purpose. For example, it can be manufactured by a formulation such as a soft lotion, a nutritional lotion, a massage cream, an essence, a nutrition cream, a pack, and the like, but is not limited thereto.

The pharmaceutical compositions may be in a transdermal dosage form such as, but not limited to, lotions, ointments, gels, creams, patches or sprays.

In the composition for external application for skin according to each formulation, components other than the composition of the present invention may be mixed and selected without difficulty by those skilled in the art depending on the formulation or use purpose of other external preparation for skin, etc. In this case, A synergistic effect can occur.

In addition, when the composition according to the present invention is used as a pharmaceutical composition, it may further contain a preservative, a stabilizer, a wetting agent or an emulsifying accelerator, a pharmaceutical adjuvant such as a salt or buffer for controlling osmotic pressure and other therapeutically useful substances And can be formulated into various oral or parenteral dosage forms according to conventional methods.

It should be understood that the actual dosage of the active ingredient should be determined in light of various relevant factors such as the severity of the symptoms, the route of administration selected, the age, sex, weight and health status of the subject.

Generally, the dosage of active ingredient is in the range of 0.001 mg / kg / day to about 2000 mg / kg / day. A more preferred dosage is 0.5 mg / kg / day to 2.5 mg / kg / day.

In addition, one embodiment of the present invention provides a skin cosmetic method by promoting skin cell proliferation, which comprises treating a skin with a composition containing a succulent callus extract.

The application site of the composition according to the present invention includes all the adipose tissues of the body and may include, for example, a face region having fewer fat layers, though it is not limited to a specific region.

The skin cell proliferation by the composition according to the present invention can be carried out in vitro, and the proliferated skin cells can be transplanted into an individual in need of corneal stem cells.

The effect of increasing the fat tissue by the composition according to the present invention includes, but is not limited to, skin elasticity increasing effect, skin wrinkle improving effect, skin regeneration effect, skin aging prevention effect,

Hereinafter, the present invention will be described in more detail with reference to the following Examples and Test Examples, which should not be construed as limiting the scope of the present invention.

[Test Example 1] Cytotoxicity of extract of Calli extract

In order to understand the cytotoxicity of the composition containing the extract of Calli extract, cytotoxicity tests on human adipose-derived stem cells and dermal keratinocytes were carried out.

(1) cytotoxicity in human adipose-derived stem cells

Human adipose derived stem cells (hADSCs) were purchased from Lonza, Inc. (Walkersville, MD, USA). The human adipose-derived stem cells were subcultured in a 75 cm ² T-flask and cultured in a CO ₂ incubator (CO ₂ Incubator) at 37 ° C and 5% CO ₂ . Typically, subculture of the cells was performed in the second or third cycle.

Derived stem cells at a concentration of 0.0001, 0.001, 0.01, 0.1, 1, 5, 10%, and the degree of cytotoxicity in adipose derived stem cells was measured by CCK-8 kit (Dojindo) Respectively.

The experimental results are shown in Fig. As can be seen from FIG. 1, it was confirmed that there was no or very little cytotoxicity at concentrations of 0.0001, 0.001, 0.01, 0.1, 1, 5, and 10%

(2) Cytotoxicity in dermal keratinocytes

Human keratinocytes were purchased from Lonza, Inc. (Walkersville, MD, USA). The keratinocytes were subcultured in a 75 cm ² T-flask and cultured in a CO ₂ incubator (CO ₂ Incubator) at 37 ° C and 5% CO ₂ . Typically, subculture of the cells was performed in the second or third cycle.

The extracts of Sequoia callus were treated with 0.0001, 0.001, 0.01, 0.1, 1, 5, and 10% concentrations of dermal keratinocytes and the degree of cytotoxicity in dermal keratinocytes was confirmed using a CCK-8 kit (Dojindo) .

The experimental results are shown in Fig. As can be seen from FIG. 2, it was confirmed that there is no or very little cytotoxicity at the concentrations of 0.0001, 0.001, 0.01, 0.1, 1, 5, and 10%.

[Test Example 2] Measurement of the expression level of the cell marker marker gene of the extract of Calli extract

In order to understand the effect of the composition containing the extract of Calli extract, the level of the marker marker gene in each of human adipose-derived stem cells and metastasis-amplified cells was measured. Human adipose stem cells (hADSCs) and keratinocytes were purchased from Lonza, Inc. (Walkersville, MD, USA).

(1) the expression of adipose derived stem cells CD29 , CD44  Measurement of expression level

In order to investigate the effect of stem cell on the stem cell of adipose - derived stem cells, the extract was supplemented with 0.001% of Sequiure callus extract. Oxidative stress of adipose derived stem cells was treated with hydrogen peroxide (H ₂ O ₂ ) at a concentration of ₂₀₀ μM for 24 hours.

Cells were washed with 2 ml of phosphate buffered saline (PBS), and then RNA was isolated from the cells using a Trizol reagent (Invitrogen, Carlsbad, Calif., USA). The separated RNA was further purified with an RNA kit (QIAGEN RNeasykt, QIAGEN, Valencia, Calif.) From Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) ) Was used to confirm the quality of the RNA. Reverse transcription polymerase chain reaction (RT-PCR) was performed using the reverse transcription kit (Invitrogen, Carlsbad, Calif.) Of Invitrogen. Q-RT-PCR).

(CD29 gene primer: Hs00559595_m1, CD44 gene primer: Hs01075861_m1) in adipide biosystems, TaqMan gene expression assay kit (Applied Biosystems, Foster City, CA) ), And the results are shown in Fig. 3 ((a) CD29, (b) CD44).

(2) the keratinocyte CD71  Measurement of expression level

The skin keratinocytes were irradiated with UVB at 100 mJ / cm 2 and treated with 0.001% of Sequoia callus extract.

Cells were washed with 2 ml of phosphate buffered saline (PBS), and then RNA was isolated from the cells using a Trizol reagent (Invitrogen, Carlsbad, Calif., USA). The separated RNA was further purified with an RNA kit (QIAGEN RNeasykt, QIAGEN, Valencia, Calif.) From Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) ) Was used to confirm the quality of the RNA. Reverse transcription polymerase chain reaction (RT-PCR) was performed using the reverse transcription kit (Invitrogen, Carlsbad, Calif.) Of Invitrogen. Q-RT-PCR).

Changes in expression patterns of genes in keratinocytes were measured using Applied Biosystems' TaqMan gene expression system (TaqMan gene expression assay kit, Applied Biosystems, Foster City, CA) (CD71 gene: Hs00951083_m1). The results are shown in FIG.

Statistics were verified by bilateral student t test and indicated significant difference when p value was less than 0.05 (marked with *).

As can be seen from FIGS. 3 and 4, the expression levels of respective genes (CD29, CD44, CD71) in adipose derived stem cells and keratinocytes were increased by about 1.53 times, 1.5 times and 1.7 times Respectively. This increase was statistically significant.

[Test Example 3] Flow cytometry analysis of adipose derived stem cells

Human adipose-derived stem cells were isolated from a 75 cm ² Ti flask with 0.25% trypsin-EDTA, and then the cells were allowed to settle at 1500 rpm for 5 minutes. After removing the culture medium, 3 ml of blocking buffer, 2% fetal bovine serum The cells were suspended by adding serum (Fetal calf serum: FCS) and 2% serum albumin (Bovine Serum Albumin (BSA) KGM-2).

Cells (approximately 10 ⁶ cells) were applied for 15 min at 4 ° C followed by incubation with a CD44 antibody (Cat No. 560977) (BD Pharmingen, CA, US) with FITC (Fluorescein isothiocyanate) and a phycoerythrin- (Cat No. 559882) (BD Pharmingen, CA, US) with cyanine attached thereto was added at a ratio of 100: 1, followed by reaction at 4 ° C for 45 minutes. FITC Rat IgG2a, k isotype (Cat No. 555742) and PE-Cy5 mouse IgG1 k isotype (Cat No. 555750) were also added to the cells used as the negative control, Lt; 0 > C for 45 minutes.

After the reaction was completed, the cells were washed twice with 3 ml of washing buffer (2% Bovine Serum Albumin (BSA) KGM-2), and then the cells were suspended. (A) CD29, (b) CD44, and (b) CD44, and (b) CD44, respectively, in FACSCalibur (BD Bioscience, San Jose, CA, US) Respectively.

Negative control group Sequoia callus extract Derived from CD29
Stem cells (number of cells) 3788 4228 increase % 12% Derived from CD44
Stem cells (number of cells) 5163 5360 increase % 4%

As shown in FIG. 5 and Table 1, it was confirmed that CD29 and CD44 increased by 12% and 4%, respectively, as compared with the negative control group .

Formulation Examples and Formulation Examples for the composition of the present invention are illustrated below.

[Formulation Example 1] Flexible longevity

The softened longevity formulation containing the Sequoia callus extract was prepared according to the usual preparation methods with the compositions shown in Table 2 below.

ingredient Content (% by weight) Betaine 3.0 Natto sword 3.0 Cellulose sword 0.005 ethanol 10.0 Polyoxyethylene hardened castor oil 0.2 Tocopheryl acetate 2.0 Sequoia callus extract 10 antiseptic a very small amount Pigment a very small amount incense a very small amount Purified water Balance

[Formulation Example 2] Nutritional lotion

A nutritional lotion formulation containing the above-mentioned Sequoia callus extract was prepared according to a conventional preparation method with the composition shown in Table 3 below.

ingredient Content (% by weight) Cetyl ethylene hexanoate 4.0 Cetostearyl alcohol 1.0 Chin type monostearyl stearate 1.0 Squalane 0.5 Sequoia callus extract 5.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 glycerin 5.0 Triethanolamine 0.5 Carboxyvinyl polymer 0.2 antiseptic a very small amount Pigment a very small amount incense a very small amount Purified water Balance

[Formulation Example 3] Cream

The cream formulations containing the Sequoia callus extracts were prepared according to conventional manufacturing procedures with the compositions shown in Table 4 below.

ingredient Content (% by weight) Glyceryl stearate 3.0 Polysorbate 60 1.0 Cetostearate 2.5 Wax 1.0 Squalane 2.0 Sorbitan sesquioleate 0.5 Cetyl ethyl hexanoate 0.5 Liquid paraffin 5.0 Active ingredient mixture 5.0 glycerin 3.0 Propylene glycol 3.0 Sequoia callus extract 5.0 antiseptic a very small amount Pigment a very small amount incense a very small amount Purified water Balance

[Formulation Example 1] Tablets

100 mg of the succulent callus extract according to the present invention, 400 mg of lactose, 400 mg of corn starch and 2 mg of magnesium stearate were mixed, and tablets were prepared by tableting according to a conventional preparation method of tablets.

[Formulation Example 2]

100 mg of the succulent callus extract according to the present invention, 400 mg of lactose, 400 mg of corn starch and 2 mg of magnesium stearate were mixed and filled in gelatin capsules according to the conventional preparation method of capsules to prepare capsules.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (13)

The present invention relates to a composition for enhancing stem cell function in an adipose-derived stem cell, which contains an extract of Calli extract of Sequoia as an active ingredient. The method according to claim 1,
Wherein the adipose-derived stem cells are human adipose derived stem cells (hADSCs). The method according to claim 1,
Wherein the composition increases fat tissue to increase the skin volume feeling. The method according to claim 1,
Wherein said composition acts to increase the expression level of CD29 and CD44 which are stem cell marker markers derived from adipose. delete delete delete delete The method according to claim 1,
Wherein the composition comprises 0.0001 to 10% by weight of the Sequoia callus extract in relation to the total weight of the composition. The method according to claim 1,
Wherein the composition is a skin cosmetic composition. The method according to claim 1,
Wherein the composition is a molding composition. The method according to claim 1,
Wherein the composition is an external preparation for skin. delete

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