KR101757619B1 - Preparing method of synthetic pig by a cross between Duroc and Korean native pig, and synthetic pig using the same - Google Patents
Preparing method of synthetic pig by a cross between Duroc and Korean native pig, and synthetic pig using the same Download PDFInfo
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Abstract
The present invention relates to a method for producing synthetic money by crossing of Duroc and Korean native pig, and includes the step of fixing SNP traits of genes involved in growth traits and meat quality traits, And the excellent meat quality traits of Korean native pigs. Further, by further fixing the SNP traits of the genes involved in the black staining, the growth, It can produce money.
Description
The present invention relates to a method of producing synthetic pigs, and more particularly to the production of synthetic pigs by crossing of Duroc and Korean native pigs, and more specifically, Is superior, or the method of producing synthetic money excellent in both growth quality, meat quality and sourcing, and thus synthetic money.
Traditional pigs in Korea originated from pigs inhabited in Manchuria, and can be classified as small and large according to body type, and it is the dominant theory that small breeds are settled because of lack of transportation means.
There are not many records about the characteristics and characteristics of Korean traditional pigs. In 1920, the Korean traditional pigs' pigs were black with a size of 22.5 ~ 32.5kg in weight, hair was long and pointed, It is said to be robust and fertile. In other words, as Korean traditional pigs are said to be resistant to diseases and resistant to disease, and that meat is only raised, Korean native pigs have maintained their characteristics of indigenous cultivars while adapting to the domestic climate since they settled on the peninsula .
However, the number of Korean traditional pigs was gradually decreasing due to the slow growth rate and the low productivity as compared with the improved pigs from the West.
However, in the 2000s, the importance of national genetic resources became more important, and the National Livestock Academy began to restore Korean native pigs. According to this, Korean native pigs have black hair, black body, long hair, sharp head , Forehead with mountain-shaped wrinkles, long and straight nose, vertical jaws, straight jaws, and ears are defined as individuals that are upright.
Nonetheless, the quality of Korean traditional pork is good, but it is still slow in growth rate and low productivity, so it is getting out of the swine industry. Currently, all of the domestic piglets depend on imports from foreign countries, and they spend huge amounts of foreign currency every year. In order to solve these problems, there is a need to actively study and utilize Korean native pigs in Korea.
On the other hand, recently, to improve the quality of pork meat, pork is scientifically managed and the pork meat obtained from the pork meat is branded, and various brand pork meat is already commercially sold. However, brand pork and pork are difficult to identify with the naked eye, so they are used to fool the pork loin, which is causing confusion in the distribution market. In order to solve these problems, various studies have been actively conducted to establish scientific objective criteria for judging pig meat.
As part of this scientific research, we are trying to develop and develop pig breeds through various methods using genetic analysis to identify the brand name or PCR (polymerase chain reaction) technology. For example, in recent patent publication No. 10-2014-0118479, a method of manufacturing a pig having excellent meat quality was patented using a large-capacity SNP chip analysis method, and in a registered patent 10-1359566, In this study, we investigated early selection method and evaluation method to find out single base polymorphism that can early select the content of pork ribs, ribs, sirloin, and seagulls by genetic tests. In the registered patent 10-1253293, 10-1270091, Type and meat quality. In addition, major genes associated with economic traits in pigs have been identified by many studies to date. The gene locus (QTL) associated with the fat content of pigs is known as
Under these circumstances, the inventors of the present invention have made efforts to develop pig varieties which are black, fast growing and improved in quality, and as a result, the SNP of the KIT gene determining the search for pigs and the SNP of the MC4R gene determining the growth trait, The present inventors completed the present invention after confirming that SNPs of the PRKAG3 gene can be stably fixed to produce synthetic black pigments which are black, fast growing and improved in quality.
Disclosure of the Invention The present invention has been made to solve the above-mentioned problems, and it is an object of the present invention to provide a synthetic monoclonal antibody which comprises a step of immobilizing SNP traits of genes involved in growth traits and meat traits by crossing of Duroc and Korean native pig In order to provide a production method.
The objective of the present invention is to produce synthetic money having both excellent growth traits of Korean native pigs and excellent meat quality traits of Korean native pigs, and further to provide synthetic money having excellent growth characteristics, meat quality, and searchability.
In order to accomplish the above object, the present invention provides a method of producing synthetic money by hybridization of Duroc and Korean native pig, wherein the SNP traits of genes involved in growth traits and meat quality traits are fixed The method comprising the steps of:
In addition, as another embodiment of the present invention, there is provided a method for producing synthetic money comprising the steps of (a) crossing a haplotype entity having A and A or G as a SNP of MC4R gene and isolating SNPs of the MC4R gene Selecting a first entity fixed to A; (b) crossing a haplotype entity having G and G or G and A as a SNP of the PRKAG3 gene, and selecting a second individual in which the SNP of the PRKAG3 gene is fixed to G among the progeny obtained therefrom; (c) crossing a haplotype entity having T and T or T and C as a SNP of the KIT gene, and selecting a third entity in which the SNP of the KIT gene is fixed to T among the offspring obtained therefrom; And (d) crossing an individual selected from the group consisting of the selected first to third individuals so that the SNP of the MC4R gene is fixed to A, the SNP of the PRKAG3 gene is fixed to G, the SNP of the KIT gene is And a step of selecting pigs fixed with " T ".
Further, another embodiment of the present invention is a synthetic money produced by the method as described above, characterized by improved growth traits and meat quality traits.
The details of other embodiments are included in the detailed description and drawings.
The present invention can provide a method of producing synthetic money comprising the step of fixing SNP traits of genes involved in growth traits and meat quality traits by crossing between Duroc and Korean native pig .
In addition, the present invention can produce synthetic pigs having both excellent growth traits of Korean native pigs and excellent meat quality traits of Korean native pigs, and furthermore, by fixing SNP traits of genes involved in black traits, growth, All have the effect of producing excellent synthetic money.
BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic diagram illustrating a cross-breeding scheme between Korean native pigs and Korean native pigs according to an embodiment of the present invention.
FIG. 2 is a graph showing the daily gain (A), the day (B), and the body length (C) of 90 kg of synthetic pigs according to an embodiment of the present invention and a comparative example of the other pigs (Crab, Korean pig, Landrace, Graphs and pictures.
FIG. 3 is a photograph showing the results of SNP genotyping analysis of the KIT gene which is related to the search for the present invention according to the present invention.
4 is a graph showing SNP genotype analysis results of the MC4R gene related to growth according to the present invention.
FIG. 5 is a graph showing the results of analysis of SNP genotype of PRKAG3 gene which is related to meat quality according to the present invention.
BRIEF DESCRIPTION OF THE DRAWINGS The present invention is capable of various modifications and various embodiments, and specific embodiments are illustrated in the drawings and will be described in detail in the detailed description. It is to be understood, however, that the invention is not to be limited to the specific embodiments, but includes all modifications, equivalents, and alternatives falling within the spirit and scope of the invention. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.
The terminology used in this application is used only to describe a specific embodiment and is not intended to limit the invention. The singular expressions include plural expressions unless the context clearly dictates otherwise. In the present application, the terms "comprises" or "having" and the like are used to specify that there is a feature, a number, a step, an operation, an element, a component or a combination thereof described in the specification, But do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.
The terms first, second, etc. may be used to describe various components, but the components should not be limited by the terms. The terms are used only for the purpose of distinguishing one component from another.
BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic diagram illustrating a cross-breeding scheme between Korean native pigs and Korean native pigs according to an embodiment of the present invention.
Rural Development Administration The National Livestock Research Institute of the Rural Development Administration has started the collection of basic pig money (9 pairs: 5 females, 4 males) for the restoration of the original native pig which was in the crisis of destruction and the genetic resource in 1988, In 2008. In 2008, the cultivars were certified as native varieties and the variety registration was completed. However, it is a fact that farmers are avoiding the breeding of native pork, which is a native breed of pig, due to the economic weakness caused by the low growing traits of traditional pork.
Accordingly, the present inventors intend to improve the utilization of conventional pigs by studying the development of synthetic pigs (synthetic black pigs) using their own native pigs.
The present invention relates to a method for producing synthetic money by crossing of Duroc and Korean native pig and fixing the SNP traits of genes involved in growth traits and meat quality traits To produce synthetic money.
First, in the present specification, "drunk" means a duroc or drunk species of a pig, and is a concept including a Duroc jersey species or a Korean dukeduckle. The Durock jersey is a species of pig belonging to a large species, ranging in variety from reddish to light red. The head is relatively small, the face is slightly concave but close to a straight line, and the ear is upright in the front, but its tip is down. The above-mentioned shrinking duroc has the meaning of promoting the livestock industry as the trade name of the product which is developed by the National Livestock Academy. The above-mentioned shrinking duroc was developed in accordance with the demand of the consumer for the boar development to differentiate from the demand and the imported pork. The shrinking duroc was completed in 1997 and started to be established in 1998 and finished trademark registration in the Korean Intellectual Property Office in 2008. Since then, it has been distributed to the National Excellent Pig Artificial Insemination Center and is mainly used for producing pork. Genus Duroc is superior to other species in terms of growth rate and growth rate, and dwarf is characterized by better meat quality than common Duroc species.
Also, in the present specification, the term "Korean native pig" is a breed of pig living on the peninsula, and is a mammal with a carp. In general, it is defined as a black body, a black body, a long, pointed head, a mountain-shaped wrinkle on the forehead, a long and straight nose, a vertical jaw, a straight jaw, have. The Korean traditional pork may include general pork, black pork, and native pork of Jeju.
In the present specification, the term "synthetic money" is a concept including a progeny pig born by crossing between different species or species, genus, or breed. In addition to simple hybrids, it may also mean a hybrid of genetically fixed varieties or lineages (hybrid money), or a breed that breeds three or more different varieties. These synthetic pigments show a fixed genetic ability, while others can be used as new varieties.
In the present specification, the term "growth trait" refers to various expression traits related to the growth of pigs, for example, growth rate, growth rate, daily gain amount, reaching age of 90 kg, and the like.
In the present specification, the term " gene involved in growth trait "refers to a gene that affects the growth traits of pigs, and the gene is not particularly limited, but is preferably a MC4R (Melanocortin-4 receptor) .
In the present specification, the term "MC4R gene" refers to a gene that exists on chromosome 1 of a pig and affects growth and feed efficiency. It expresses mainly a G-protein pair receptor important for energy center control, It is known to play a crucial role in the genetic basis of regulation and obesity (Farooqi et al., 2003). The MC4R gene may preferably comprise the polynucleotide of SEQ ID NO: 1. In the present invention, pigs having an excellent growth rate can be obtained by having an A (Asn) allele as an Asp298Asn single base mutation in the porcine MC4R gene. That is, according to the present invention, it is more preferable that the SNP of the gene involved in the growth trait is A at position 298 of the MC4R gene.
Herein, the term "meat quality trait" refers to various expression traits indicating the state of a slaughtered product obtained from a pig. For example, the meat quality such as Ph, meat color, water holding capacity, heating weight loss, muscle fat content, shearing force, And is not particularly limited thereto.
In the present specification, the term " gene involved in the meat quality trait "refers to a gene that affects the meat quality of pigs. Although the gene is not particularly limited, PRKAG3 (AMP-activated protein kinase, gamma 3 subunit gene) gene and the like.
In the present specification, the term "PRKAG3 gene" refers to a gene that exists on the chromosome 15 of a pig and produces a protein that regulates the glycogen content of the pig muscle. The PRKAG3 gene may preferably comprise the polynucleotide of SEQ ID NO: 2. It is known that G / A SNP exists at base 1846 on the basis of the ATG (start codon) of the PRKAG3 gene. In the present invention, when the 1846th base is G, Pork meat with excellent quality can be obtained.
Basically, the present invention relates to a method for producing synthetic money by crossing Duroc and Korean native pig. Generally, it is known that the Crabs are excellent in growth traits and the Korean native pigs are excellent in meat quality. Therefore, the present inventors have tried to produce synthetic pigs having merits of Korean native pigs and merits of Korean native pigs. In other words, the present invention is not only superior in growth rate but also produce synthetic pig excellent in meat quality like Korean native pig.
Thus, in the present invention, synthetic pigs were produced by crossing between Korean native pigs and Korean native pigs, and synthetic pigs having excellent growth rate and excellent meat quality could be produced by fixing the SNP traits of the genes specifically involved in growth traits and meat quality traits . The SNP trait fixation of the gene can be performed by selecting a homozygote having all of the SNPs through a genotyping method (PCR-RFLP, Sequencing) of the SNPs. For example, it can be performed by crossing an individual having a haplotype having all of the above-mentioned SNP traits and selecting a homozygote having a desired trait.
In the present invention, it is preferable to cross the Korean native pig with the Korean native pig, and more preferably, the hybrid first female and the Korean native male obtained by crossing the Korean domestic pig male with the Korean native pig, It is suitable to obtain a second-generation hybrid female, and to obtain a second-generation female hybrid and the first-generation male hybrid.
According to this hybridization method, synthetic money having a Korean native pig blood ratio of 37.5% can be obtained (see FIG. 1). In the case of synthetic pigs according to the present invention, when the blood ratio of Korean native pigs is 37.5%, the fresh weight, color coordinate (red), water retention, heat loss and shearing force (year) I was able to get excellent objects.
In another aspect of the present invention, there is provided a method of isolating SNPs comprising the steps of: (a) fixing an SNP trait of a gene involved in black trafficking, wherein the gene involved in the growth trait and the meat trait is a gene involved in growth traits, have.
In the present specification, "a gene involved in black coloring" means a gene that affects pig color of a pig, and the gene is not particularly limited thereto, but may be a KIT gene or the like.
The term "KIT gene " of the present invention refers to a gene coding for a protein that determines the mast / stem cell growth factor receptor, such as pigs, cows, horses and dogs. , It has 21 exons, has a length of 2,919 bp based on the mRNA base sequence, and has a length of 949 aa based on the amino acid sequence. The nucleotide sequence of the KIT gene can be obtained from a known database such as NCBI's GenBank. May be a gene represented by NG_007456.1, preferably a polynucleotide of SEQ ID NO: 3. It is known that the KIT gene functions as an essential factor for survival such as control, survival, death and proliferation of melanocyte differentiation, and that the mutation of the KIT gene is related to gastrointestinal stromal tumor, mast cell disease, staining albinism. In particular, the KIT gene of pigs is useful for distinguishing color and white. KIT gene of white pig has CNV (copy number variation) in two or more genes, but it has black color and red color Pigs are known to have only one KIT gene. When the base corresponding to the 586th base of the KIT gene is T, improved pig coloration can be exhibited.
In addition, as another embodiment of the present invention, there is provided a method for producing synthetic money comprising the steps of (a) crossing a haplotype entity having A and A or G as a SNP of MC4R gene and isolating SNPs of the MC4R gene Selecting a first entity fixed to A; (b) crossing a haplotype entity having G and G or G and A as a SNP of the PRKAG3 gene, and selecting a second individual in which the SNP of the PRKAG3 gene is fixed to G among the progeny obtained therefrom; (c) crossing a haplotype entity having T and T or T and C as a SNP of the KIT gene, and selecting a third entity in which the SNP of the KIT gene is fixed to T among the offspring obtained therefrom; And (d) crossing an individual selected from the group consisting of the selected first to third individuals so that the SNP of the MC4R gene is fixed to A, the SNP of the PRKAG3 gene is fixed to G, the SNP of the KIT gene is And a step of selecting pigs fixed with " T ".
Here, in the step of selecting the third entity, it is preferable to select a homozygote having a length of 136 bp and 50 bp as a result of PCR-RFLP analysis of the KIT gene.
In addition, the first individual may have an As (Asn) allele as an Asp298Asn single base mutation in the MC4R gene.
In addition, the second entity may have G as the 1846th nucleotide based on the ATG start codon in the PRKAG3 gene.
Further, it is more preferable that the third entity has T as the 586th base in the KIT gene.
According to one embodiment of the present invention, the genotype of the MC4R gene (FIG. 4) and the PRKAG3 gene (FIG. 5) involved in the growth and meat quality of pigs can be confirmed by direct sequence analysis, (Fig. 2), meat quality (Table 2), and synthetic black pig (black pig) with improved black stain.
Further, another embodiment of the present invention is a synthetic money produced by the method as described above, characterized by improved growth traits and meat quality traits.
In addition, the present invention may be a synthetic money produced by the above-mentioned method and characterized by improved growth traits, meat quality traits and black discoloration.
According to the present invention, the SNPs of the genes involved in the growth traits, the meat quality traits and / or the black traits of pigs are fixed to specific traits so that the growth traits, the meat traits and / or the black traits of the pigs show the best level Improved pigs can be produced. For the purpose of the present invention, the modified pig includes a state in which the SNPs present in the MC4R gene, the PRKAG3 gene and / or the KIT gene, which are genes involved in the growth traits, the meat quality traits and / or the black traits of pigs, However, it is not particularly limited thereto.
The synthetic money according to the present invention may be a pig produced by the above-mentioned crossing method, or a gene in which a SNP of a gene involved in growth traits, meat quality traits and / or black traits is fixed to a specific trait, The transgenic embryo can be a transgenic pig produced using transgenic embryos prepared by introducing the transgenic embryo. As a method for transducing the gene into the fertilized egg, a biochemical method, a physical method, or a virus-mediated transfection method may be used. Preferably, the transfection method is carried out using FuGene6 (Roche, USA),
The present invention may be better understood by the following examples, which are for the purpose of illustrating the invention and are not intended to limit the scope of protection defined by the appended claims.
Example
One:
Foundation
Furtherance
Among the pig breeds, the first generation of hybrid, which was produced by crossing the females of the dulox species, which are the most commonly used paternal pigs, and the male of the native pigs, were used as the breed axes of the next generation. The hybrid second-generation females were crossed again with the males of the first generation, and the basic females with the normal pig blood ratio of 37.5% were formed as shown in Fig.
Example
2: Genes involved in swine growth
SNP
Genotype fixation of
The genotypes of gene SNPs involved in growth were analyzed by direct sequencing using the basal gang of Example 1.
FIG. 2 is a graph showing the daily gain (A), the day (B), and the body length (C) of 90 kg of synthetic pigs according to an embodiment of the present invention and a comparative example of the other pigs (Crab, Korean pig, Landrace, FIG. 3 is a graph showing SNP genotype analysis results of the MC4R gene related to growth according to the present invention. FIG.
As shown in Fig. 3, three genotypes were detected, and homozygous individuals having the A / A genotype were selected. According to this, as shown in FIG. 2, it was found that when a genotype of MC4R gene SNP is genetically fixed by crossing an individual having A / A, it is possible to produce a fast growing pig.
Example
3: pig
On meat quality
Involved genes
SNP
Genotype fixation of
Genotypes of gene SNPs involved in meat quality were analyzed by direct sequencing using the base group of Example 1.
FIG. 4 is a graph showing the SNP genotype analysis results of the PRKAG3 gene which is related to the meat quality according to the present invention. As shown here, three genotypes were detected and homozygous individuals with the G / G genotype were selected. According to this, as shown in Table 2, it can be seen that when the genotype of the PRKAG3 gene SNP is genetically fixed through hybridization, a pig having good quality can be produced.
Example
4: Of pigs
In search of black
Involved gene
SNP
Genotype fixation of
The genotypes of gene SNPs involved in black staining were analyzed by the PCR-RFLP method using the basal gang of Example 1.
FIG. 5 is a photograph showing the results of SNP genotyping analysis of the KIT gene which is related to the search for the present invention according to the present invention. As shown here, two genotypes emerged, and homozygous individuals with lengths of 136 bp and 50 bp were selected. According to the results, it was found that pigs exhibiting black staining can be produced when a genotype of KIT gene SNP has a length of 136 bp + 50 bp and is genetically fixed through crossing.
Example
5:
Of synthetic pork (synthetic black pork)
Produce
A KIT gene SNP genotype of 136 bp + 50 bp was crossed to a KIT gene SNP-fixed pig using the basal group of Example 1, and the MC4R gene SNP genotype was A / A or A / G Were selected for breeding of pigs with MC4R gene SNPs fixed at A / A. PRKAG3 gene SNPs were crossed with individuals with G / G or G / A genotype so that PRKAG3 gene SNPs were fixed to G / G Pigs were selected.
Each of the selected pigs was then mated sequentially to finally obtain a pig having a KIT gene SNP of 136 bp + 50 bp and MC4R gene SNP fixed at A / A and PRKAG3 gene SNP fixed at G / G And we named it "Black".
Experimental Example
1: sensory evaluation of sirloin
In order to evaluate the meat quality of the Korean black beet according to Example 5, sensory evaluation was performed on the beef areas of native pigs, synthetic black pigs and general finishing pigs (LYD, triad hybrids).
* The different alphabets between the same lines are statistically significant at 5% level
It is very good (6), good (5), slightly good (4), slightly dislike (3), dislike (2)
As shown in Table 1 above, the synthetic black pork (black pig) produced by the method according to the present invention showed almost the same results as the conventional pigs, and it was found that the pig pork was superior to the general pig pig.
Experimental Example
2 :
Conductor grade
analysis(
Meat quality
analysis)
In order to evaluate the meat quality of the Korean black beet produced according to Example 5, the conductance of traditional pig and finishing pigs (LYD) which were similarly raised at the National Livestock Academy was analyzed and compared.
As shown in Table 2, the synthetic black pork (Korean black pig) prepared by the method of the present invention showed superior color to the regular pig meat, and it was confirmed that it was superior to the conventional pig in terms of pH and water holding capacity.
Although the present invention has been shown and described with respect to certain preferred embodiments thereof, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the following claims It will be apparent to those skilled in the art.
<110> REPUBLIC OF KOREA (MANAGEMENT: RURAL DEVELOPMENT ADMINISTRATION) <120> Preparing method of synthetic pig by a cross between Duroc and Korean native pig, and synthetic pig using the same <130> PA-D15079 <160> 3 <170> Kopatentin 2.0 <210> 1 <211> 999 <212> DNA <213> The MC4R gene <400> 1 atgaactcaa cccatcacca tggaatgcat acttctctcc acttctggaa ccgcagcacc 60 tacggactgc acagcaatgc cagtgagccc cttggaaaag gctactctga aggaggatgc 120 tacgagcaac tttttgtctc tcctgaggtg tttgtgactc tgggtgtcat aagcttgttg 180 gagaacattc tggtgattgt ggccatagcc aagaacaaga atctgcattc acccatgtac 240 tttttcatct gtagcctggc tgtggctgat atgctggtga gcgtttccaa tgggtcagaa 300 accattgtca tcaccctatt aaacagcacg gacacggacg cacagagttt cacagtgaat 360 attgataatg tcattgactc agtgatctgt agctccttac tcgcctcaat ttgcagcctg 420 ctttcgattg cagtggacag gtattttact atcttttatg ctctccagta ccataacatt 480 atgacagtta agcgggttgg aatcatcatc agttgtatct gggcagtctg cacggtgtcg 540 ggtgttttgt tcatcattta ctcagatagc agtgctgtta ttatctgcct cataaccgtg 600 ttcttcacca tgctggctct catggcttct ctctatgtcc acatgttcct catggccaga 660 ctccacatta agaggatcgc cgtcctccca ggcactggca ccatccgcca aggtgccaac 720 atgaaggggg caattaccct gaccatcttg attggggtct ttgtggtctg ctgggccccc 780 ttcttcctcc acttaatatt ctatatctcc tgcccccaga atccatactg tgtgtgcttc 840 atgtctcact ttaatttgta tctcatcctg atcatgtgta attccatcat cgatcccctg 900 atttatgcac tccggagcca agaactgagg aaaaccttca aagagatcat ctgttgctat 960 cccctgggtg gcctctgtga tttgtctagc agatattaa 999 <210> 2 <211> 2023 <212> DNA <213> Prkag3 gene <400> 2 atggagcttg ccgagctaga gcaggcactg cgcagggtcc cggggtcccg ggggggctgg 60 gagctggagc aactgaggcc agagggcaga gggcccacca ctgcggatac tccctcctgg 120 agcagcctcg ggggacctaa gcatcaagag atgagcttcc tagagcaagg agagagccgt 180 tcatggccat cccgagctgt aaccaccagc tcagaaagaa gccatgggga ccaggggaac 240 aaggcctcta gatggacaag gcaggaggat gtagaggaag gggggcctcc gggcccgagg 300 gaaggtcccc agtccaggcc agttgctgag tccaccgggc aggaggccac attccccaag 360 gccacaccct tggcccaagc cgctcccttg gccgaggtgg acaacccccc aacagagcgg 420 gacatcctcc cctctgactg tgcagcctca gcctccgact ccaacacaga ccatctggat 480 ctgggcatag agttctcagc ctcggcggcg tcgggggatg agcttgggct ggtggaagag 540 aagccagccc cgtgcccatc cccagaggtg ctgttaccca ggctgggctg ggatgatgag 600 ctgcagaagc cgggggccca ggtctacatg cacttcatgc aggagcacac ctgctacgat 660 gccatggcga ccagctccaa actggtcatc ttcgacacca tgctggagat caagaaggcc 720 ttctttgccc tggtggccaa cggcgtccga gcggcacctt tgtgggacag caagaagcag 780 agcttcgtgg ggatgctgac catcacagac ttcatcttgg tgctgcaccg ctattacagg 840 tcccccctgg tccagatcta cgagattgaa gaacataaga ttgagacctg gagggagatc 900 taccttcaag gctgcttcaa gcctctggtc tccatctctc ccaatgacag cctgttcgaa 960 gctgtctacg ccctcatcaa gaaccggatc caccgcctgc cggtcctgga ccctgtctcc 1020 ggggctgtgc tccacatcct cacacataag cggcttctca agttcctgca catctttggc 1080 accctgctgc cccggccctc cttcctctac cgcaccatcc aagatttggg catcggcaca 1140 ttccgagact tggccgtggt gctggaaacg gcgcccatcc tgaccgcact ggacatcttc 1200 gtggaccggc gtgtgtctgc gctgcctgtg gtcaacgaaa ctggacaggt agtgggcctc 1260 tactctcgct ttgatgtgat ccacctggct gcccaacaaa catacaacca cctggacatg 1320 aatgtgggag aagccctgag gcagcggaca ctgtgtctgg aaggcgtcct ttcctgccag 1380 ccccacgaga ccttggggga agtcattgac cggattgtcc gggaacaggt gcaccgcctg 1440 gtgctcgtgg atgagaccca gcaccttctg ggcgtggtgt ccctctctga catccttcag 1500 gctctggtgc tcagccctgc tggaattgat gccctcgggg cctgagaacc ttggaacctt 1560 tgctctcagg ccacctggca cacctggaag ccagtgaagg gagccgtgga ctcagctctc 1620 acttcccctc agccccactt gctggtctgg ctcttgttca ggtaggctcc gcccggggcc 1680 cctggcctca gcatcagccc ctcagtctcc ctgggcaccc agatctcaga ctggggcacc 1740 ctgaagatgg gagtggccca gcttatagct gagcagcctt gtgaaatcta ccagcatcaa 1800 gactcactgt gggaccactg ctttgtccca ttctcagctg aaatgatgga gggcctcata 1860 agaggggtgg acagggcctg gagtagaggc cagatcagtg acgtgccttc aggacctccg 1920 gggagttaga gctgccctct ctcagttcag ttcccccctg ctgagaatgt ccctggaagg 1980 aagccagtta ataaaccttg gttggatgga atttggagag tcg 2023 <210> 3 <211> 2919 <212> DNA <213> KIT gene <400> 3 atgagaggcg ctcgccgcgc ctgggatttt ctcttcgtcc tgcagctctt gcttcgcgtc 60 cagacaggct cttctcagcc atctgtgagt ccagaggaac tgtctccacc atccatccag 120 ccagcaaaat cagagttaat cgtcagtgct ggcgatgaga ttaggctgtt ctgcaccgat 180 ccaggatctg tcaaatggac ttttgagacc ctgggtcagc tgagtgagaa tactcacgca 240 gagtggatcg tggagaaagc agaggccatg aatacaggca attatacatg caccaatgaa 300 ggcggtttaa gcagttccat ttatgtgttt gttagagatc ctgagaagct tttcctcgtc 360 gaccctccct tgtatgggaa ggaggacaat gacgcgctgg tccgctgtcc tctgacggac 420 ccagaggtga ccaattactc cctcacgggc tgcgagggga aaccccttcc caaggatttg 480 accttcgttg cagaccccaa ggccggcatc accatcaaaa atgtgaagcg cgagtatcat 540 cggctgtgtc tacactgctc cgccaaccag gggggcaagt ccgtgctgtc gaagaaattc 600 accctgaaag tgagggcagc catcagagct gtacctgttg tggctgtgtc caaagcaagc 660 taccttctca gggaagggga ggaatttgcc gtgatgtgct tgatcaaaga cgtgtctagt 720 tccgtggact ccatgtggat cagggagaac agccagacta aagcacaggt gaagaggaat 780 agctggcatc agggtgactt caattttctg cggcaggaaa agctgacgat cagctcagca 840 agagttaatg attctggcgt gttcatgtgt tacgccaata atacttttgg atctgcaaat 900 gtcacaacca ccttagaagt agtagataaa ggattcatta atatcttccc tatgatgaat 960 accactgtgt ttgtaaacga tggagaggat gtggatctaa ttgttgagta cgaggcgtac 1020 cccaaacctg aacaccgaca gtggatatat atgaaccgca ctgccactga taagtgggag 1080 gattatccca agtctgagaa tgaaagtaac atcagatatg taagtgaact tcacttgacc 1140 agattaaaag ggaccgaagg aggcacttac acatttctcg tgtccaatgc tgatgtcaat 1200 tcttctgtga catttaatgt ttacgtgaac acaaaaccag aaatcctgac tcatgacagg 1260 ctcatgaacg gcatgctcca gtgtgtggcg gcaggcttcc cagagcccac catcgattgg 1320 tatttctgtc caggcaccga gcagagatgt tccgttcccg ttgggccagt ggacgtgcag 1380 atccaaaact catctgtatc accgtttgga aaactagtga ttcacagctc cattgattac 1440 agtgcattca aacacaacgg cacggtggag tgcagggctt acaacgatgt gggcaagagt 1500 tctgcctttt ttaactttgc atttaaagaa caaatccatg cccacaccct cttcacgcct 1560 ttgctgattg gttttgtgat cgcagcgggt atgatgtgta tcatcgtgat gattctcacc 1620 tataaatatc tacagaagcc catgtatgaa gtacagtgga aggttgtcga ggagataaat 1680 ggaaacaatt atgtctacat agacccaacg caacttcctt atgatcacaa atgggaattt 1740 cccaggaaca ggctgagttt tggcaaaacc ttgggtgctg gcgccttcgg gaaagtcgtt 1800 gaggccactg catacggctt aattaagtca gatgcggcca tgaccgttgc cgtgaagatg 1860 ctcaaaccaa gtgcccattt aacggaacga gaagccctaa tgtctgaact caaagtctta 1920 agttacctcg gtaatcacat gaatattgtg aatcttctcg gcgcctgcac cattggaggg 1980 cccaccctgg tcattacaga atattgttgc tatggtgatc tcctgaattt tttgagacgg 2040 aaacgtgatt cgtttatttg ctcaaagcag gaagatcacg cagaagcggc gctttataag 2100 aaccttctgc attcaaagga gtcttcctgc agtgacagta ctaacgagta catggacatg 2160 aaacccggag tgtcttatgt ggtaccaacc aaggcagaca aaaggagatc tgcgagaata 2220 ggctcataca tagaacgaga tgtgactcct gccatcatgg aagatgatga gttggcccta 2280 gacctggagg acttgctcag cttttcttac caagtggcaa agggcatggc cttcctcgcc 2340 tcgaagaatt gtattcacag agacttggcg gccagaaata tcctccttac tcatggtcga 2400 atcacaaaga tttgtgattt tggtctagcc agagacatca agaatgattc taattacgtg 2460 gtcaaaggaa acgctcggct acccgtgaag tggatggcac ccgagagcat tttcaactgt 2520 gtctacacat ttgaaagcga tgtctggtcc tatgggattt ttctgtggga gctcttctct 2580 ttagggagca gcccctaccc tggaatgcca gttgattcta aattctacaa gatgatcaag 2640 gagggtttcc gaatgctcag ccctgagcat gcacctgcgg aaatgtatga catcatgaag 2700 acttgctggg atgcggatcc cctcaaaaga ccaacgttta agcagattgt gcagctgatt 2760 gagaagcaga tttcggagag caccaatcac atttattcca acttagcgaa ctgcagcccc 2820 caccgggaga accccgcggt ggatcattct gtgcggatca actccgtggg cagcagtgcc 2880 tcctccacgc agccgctgct tgtccacgaa gacgtctga 2919
Claims (16)
And fixing the SNP trait of the gene involved in the growth trait and the meat trait,
The mating of the Korean native pig with the Korean native pig is carried out by crossing the first hybrid male and the second male male obtained by crossing the female and the Korean native pig with each other to obtain a hybrid second generation female, It is to abandon the generation male,
Wherein the synthetic pig has a Korean native pig blood ratio of 37.5%.
Wherein the SNP of the gene involved in the growth trait is A of the 298th base of the MC4R (Melanocortin-4 receptor) gene.
Wherein the SNP of the gene involved in the meat quality trait is G at the 1846th base of the PRKAG3 (AMP-activated protein kinase, gamma 3 subunit gene) gene.
And fixing the SNP trait of the gene involved in the black embryo,
Wherein the genes involved in the growth traits and meat quality traits are genes involved in growing traits, meat traits, and black traits.
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| KR102544175B1 (en) * | 2021-03-25 | 2023-06-16 | (주)티엔티리써치 | Composition for Discriminating Breeds of Pig and Uses thereof |
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| 논문 1(원정일 등, 농업생명과학연구 vol.48 no.4 pp.155-163(2014.08. 공개))* |
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