KR101754220B1 - Skin aging and atopic dermatitis improving composition including natural medicinal herbs cosmetic composition and preparation method of the same - Google Patents

Abstract

The present invention relates to a composition for skin aging, atopic dermatitis, and skin whitening comprising a natural herbal medicine extract. More specifically, the present invention relates to a composition for skin aging, atopic dermatitis, Saururus, and sulphate.
The present invention also relates to a method for preparing a composition for skin whitening comprising at least one of natural herbal extracts, skin aging, atopic dermatitis and skin whitening. More specifically, the present invention relates to (1) Preparing bacon, white mackerel, horse mackerel, mackerel, saururus and sulfur; (2) drying and pulverizing the Hashuo, mint, mashin, licorice, beeswax, Karendra, white bamboo, white bamboo, white bamboo, horse mackerel, saururus and Sulfur; And (3) extracting the powdered Sasao, mint, marjoram, licorice, beeswax, Karendra, white bream, white mackerel, horse mackerel, mallow, saururus, and sulfur on the basis of the constitution.
According to the composition for skin aging, improvement of atopic appearance and skin whitening comprising the natural herbal extracts proposed in the present invention and a method for producing the same, the composition for skin aging, atopic dermatitis, By containing the extract of wax, Karendra, bamboo shoots, bamboo shoots, bamboo shoots, bamboo shoots, saururus and Sulfur as an active ingredient, it is possible to improve the wrinkles of the skin and to treat or improve atopic skin inflammation.

Description

TECHNICAL FIELD The present invention relates to a composition for skin aging, atopic dermatitis and skin whitening comprising natural herbal extracts and a method for producing the same,

The present invention relates to a composition for skin aging, atopic dermatitis and skin whitening, and more particularly to a composition for skin aging, atopic dermatitis and skin whitening comprising natural herbal extracts and a method for producing the same.

As the economy develops and people's income levels improve, interest in health is also growing. In recent years, anti-aging trends for youth and beauty have been rapidly spreading, and there is an era in which people seek to live beautifully, not just from a long life, but from a healthy life.

In this regard, interest in the media, academia, and industry has increased as the anti-aging industry customer base such as dermatology, cosmetics, and cosmetics services expands. Skin aging is a major axis of the anti-aging industry. The skin is outside of the body, so it can be easily improved through management, compared to other organs, and it starts to measure physical health and age from skin condition, It is considered to be a key part of anti-aging.

On the other hand, the anti-aging industry is expected to have sufficient growth potential in terms of both demand and biotechnology innovation due to aging and income increase, and capital investment and technological development are expected to be boosted. However, as a result of this anti-aging hot wind, products that have not been properly validated have been circulated, resulting in consumer damage. As a result, the development of excellent products through systematic R & D, It is a very important and essential situation.

In the case of atopic dermatitis, one of the major diseases that appear on the skin, children and adolescents conducted by the Disease Control Headquarters in 41 elementary schools in 40 elementary schools in 2003 and 40 in 2003. According to the results of asthma and allergic diseases survey, Atopic dermatitis increased 2.2 times (9.2% → 20.6%) in children (elementary school 1) and 3.2 times (4.0% → 12.9%) in adolescents (middle school 1) Atopic dermatitis is increasing worldwide. In particular, the prevalence of atopic dermatitis symptoms in 12-month-old infants is increasing in most countries.

Among functional cosmetics, cosmetics derived from natural products have little side effects and are generally mild and persistent in their action, have high biodegradability and are environmentally friendly, so they have a good image among ordinary consumers and have been spurring research on the development of cosmetics derived from natural products in developed countries such as USA and Japan . However, the research on cosmetics derived from natural products in Korea is insignificant compared to advanced countries. Especially, due to changes in environmental pollution and residential environment, the population suffering from atopic skin as well as infants is increasing. In contrast, there is a need for a technique that can improve atopic symptoms and improve overall skin aging.

In this connection, Korean Patent No. 10-1470064 (Dec. 12, 2014) discloses a cream composition for treating atopic dermatitis containing an elm extract, and Korean Patent No. 10-1224519 (Feb. Discloses a cosmetic composition containing fermented collagen of Mycelium of mushroom mycelia in order to prevent aging of the skin. However, it discloses a composition for at least one of symptoms and improvement of atopic dermatitis and skin aging. At the same time, pharmaceutical compositions and cosmetic compositions capable of improving symptoms of atopic dermatitis have limitations not disclosed.

The present invention has been proposed in order to overcome the above-mentioned problems of the previously proposed methods, and it is an object of the present invention to provide a composition for skin aging, atopic dermatitis and skin whitening, which comprises at least one composition selected from the group consisting of hushuo, mint, mashin, licorice, beeswax, , Atopic dermatitis and skin whitening, including natural herbal extracts, which can be used for improving skin wrinkles and improving or treating atopic dermatitis, by containing extracts of Aspergillus oryzae, And a method for producing the same.

In order to accomplish the above object, the present invention provides a composition for skin aging, atopic dermatitis, and skin whitening comprising a natural herbal extract,

As a composition for skin aging, atopic improvement and skin whitening,

The composition of the present invention is characterized in that it contains an extract of Sasa, Mint, Mashihyun, Licorice, Beeswax, Karendra, Baekjongbyeol, Baekjangsam, Rhizophora, Rhizoma, Saururus and Sulfur as effective ingredients.

Preferably,

Wherein the mixing ratio of the mint is 11 wt% to 14 wt%, the mixing ratio of the mint is 11 wt% to 14 wt%, the mixing ratio of the mint is 8 wt% to 11 wt%, the mixing ratio of the licorice is 14 wt% To about 17% by weight, the blending ratio of the beeswax is about 8% by weight to about 11% by weight, the blending ratio of the Karendra is about 2% to about 5% by weight, the blend ratio is about 5% Wherein the blend ratio of the bamboo shoots is 2 to 5 wt%, the blend ratio of the bamboo shoots is 8 to 11 wt%, the blending ratio of the bamboo shoots is 0.1 to 3 wt%, the blend ratio of the three- To 14% by weight, and the blending ratio of sulfur is 5% by weight to 8% by weight.

Preferably,

Wherein the mixing ratio of the mint is 12 wt% to 13 wt%, the mixing ratio of the mint is 12 wt% to 13 wt%, the mixing ratio of the mint is 9 wt% to 10 wt%, the mixing ratio of the licorice is 15 wt% To 16% by weight, the mixing ratio of the beeswax is 9% by weight to 10% by weight, the mixing ratio of the Karendra is 3% to 4% by weight, the mixing ratio of the beeswax is 6% Wherein the mixing ratio of the bamboo shoots is 3 wt% to 4 wt%, the mixture ratio of the bamboo shoots is 9 wt% to 10 wt%, the mixing ratio of the perch is 1 wt% to 2 wt% To 13% by weight, and the blending ratio of sulfur is 6% by weight to 7% by weight.

According to an aspect of the present invention, there is provided a composition for improving skin aging,

The composition of the present invention is characterized in that it contains an extract of Sasa, Mint, Mashihyun, Licorice, Beeswax, Karendra, Baekjongbyeol, Baekjangsam, Rhizophora, Rhizoma, Saururus and Sulfur as effective ingredients.

According to an aspect of the present invention, there is provided an atopic composition comprising:

The composition of the present invention is characterized in that it contains an extract of Sasa, Mint, Mashihyun, Licorice, Beeswax, Karendra, Baekjongbyeol, Baekjangsam, Rhizophora, Rhizoma, Saururus and Sulfur as effective ingredients.

According to an aspect of the present invention, there is provided a composition for whitening skin,

The composition of the present invention is characterized in that it contains an extract of Sasa, Mint, Mashihyun, Licorice, Beeswax, Karendra, Baekjongbyeol, Baekjangsam, Rhizophora, Rhizoma, Saururus and Sulfur as effective ingredients.

In order to achieve the above object, the present invention provides a method for skin aging, atopic dermatitis, and skin whitening comprising a natural herbal extract,

(1) Preparing Sasa, Mint, Mashin, Licorice, Beeswax, Karendra, Baekbyeong, Baekjangjae, Rhizoma, Rhizoma, Saururus and Sulfur;

(2) drying and pulverizing the Hashuo, mint, mashin, licorice, beeswax, Karendra, white bamboo, white bamboo, white bamboo, horse mackerel, saururus and Sulfur; And

(3) It is characterized in that it includes the step of extracting the powdered Sasao, mint, mashin, licorice, beeswax, Karendra, white bream, white mackerel, horse mackerel,

Preferably,

(4) freezing and drying the extracted Hansuo, mint, marjoram, licorice, beeswax, Karendra, white bream, white mackerel, horse mackerel, mallow, saururus and sulfur.

According to the composition for skin aging, improvement of atopic appearance and skin whitening comprising the natural herbal extracts proposed in the present invention and their preparation method, the composition for skin aging, atopic dermatitis and skin whitening comprising natural herbal extracts, The present invention can improve the skin wrinkles and improve or treat the atopic skin inflammation by containing the extract as an active ingredient.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is an explanatory diagram for explaining skin aging according to sun exposure; FIG.
Figure 2 illustrates remodeling of extracellular matrix (ECM) of endogenous skin aging and exogenous skin aging.
FIG. 3 shows the effect of TRPV1 on the increase of MMP1 expression by ultraviolet exposure. FIG.
FIG. 4 conceptually illustrates the mechanism of immunopathology of atopic dermatitis. FIG.
FIG. 5 is a view showing the composition of skin aging, atopic improvement, and skin whitening composition containing a natural herbal extract according to an embodiment of the present invention. FIG.
6 is a view showing the results of a test example of DPPH radical scavenging activity of skin aging, atopic improvement, and skin whitening composition containing a natural herbal extract according to an embodiment of the present invention.
7 is a graph showing the results of a test example of skin aging, atopic improvement, and lipid peroxidation inhibitory activity of a composition for skin whitening comprising natural herbal extracts according to an embodiment of the present invention.
8 is a view showing the results of a test example of skin anaglyph, atopic skin improvement, and peroxide anion scavenging activity of a skin whitening composition containing a natural herbal extract according to an embodiment of the present invention.
9 is a view showing the results of a test example of skin aging, atopic improvement, and skin whitening inhibitory activity of natural herb extracts according to one embodiment of the present invention.
10 is a graph showing the results of a test example of skin aging, atopic improvement, and tyrosinase inhibitory activity of a composition for skin whitening comprising a natural herbal extract according to an embodiment of the present invention.
11 is a view showing the result of a cytotoxicity test example of skin aging, atopic improvement, and skin whitening composition containing a natural herbal extract according to an embodiment of the present invention.
FIG. 12 is a view showing a device configuration for evaluating skin ion passage control efficacy of skin aging, atopic improvement, and skin whitening composition including natural herbal extracts according to an embodiment of the present invention. FIG.
FIG. 13 is a graph showing a skin aging, atopic improvement, and skin whitening composition inhibition effect of a TRPV1 ion channel comprising a natural herbal extract according to an embodiment of the present invention. FIG.
FIG. 14 is a graph showing the skin aging, atopic improvement, and skin whitening compositions containing the natural herbal extract according to an embodiment of the present invention and the Orai1 ion channel inhibition effect. FIG.
FIG. 15 is a graph showing the skin aging, atopic improvement, and inhibition of NO production of a skin whitening composition containing a natural herbal extract according to an embodiment of the present invention. FIG.
16 is a graph showing the skin aging, atopic improvement, and PGE2 inhibitory effect of a composition for skin whitening comprising natural herbal extracts according to an embodiment of the present invention.
FIG. 17 is a graph showing the skin aging, atopic improvement, and IL-1? Inhibiting effect of a skin whitening composition containing a natural herbal extract according to an embodiment of the present invention. FIG.
FIG. 18 is a graph showing the skin aging, atopic improvement, and skin whitening composition TNF-α inhibitory effect including natural herbal extracts according to an embodiment of the present invention. FIG.
19 is a graph showing the skin aging, atopic improvement, and IL-6 inhibiting effect of a skin whitening composition containing a natural herbal extract according to an embodiment of the present invention.
20 to 23 show results of clinical application of skin aging, atopic improvement and skin whitening composition comprising natural herbal extracts according to an embodiment of the present invention to skin troubles.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those skilled in the art can easily carry out the present invention. In the following detailed description of the preferred embodiments of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear. The same or similar reference numerals are used throughout the drawings for portions having similar functions and functions.

In addition, in the entire specification, when a part is referred to as being 'connected' to another part, it may be referred to as 'indirectly connected' not only with 'directly connected' . Also, to "include" an element means that it may include other elements, rather than excluding other elements, unless specifically stated otherwise.

In the present specification, the term " active ingredient " includes a substance or a group of substances expected to directly or indirectly express the efficacy of the composition by an inherent pharmacological action (pharmacologically active ingredients, etc., Quot; means that the main component is included.

Hereinafter, the mechanism of skin aging and atopic dermatitis will be described, respectively, and the constitution and effects of the present invention proposed by the present inventors will be described in order to improve skin aging and atopic dermatitis.

Mechanism of skin aging

In view of the physiological characteristics of skin aging, skin aging is a complex biological phenomenon that can be determined by two factors, intrinsic and extrinsic. Such skin aging is mainly related to wrinkling, sagging, and skin laxity caused by aging. For example, in the case of wrinkles caused by natural aging, skin is soft and pale, whereas skin caused by photoaging due to ultraviolet rays A and B produces rough and deep wrinkles, while pigmentation and capillary dilatation There are features to be observed.

FIG. 1 is an exemplary diagram for explaining skin aging according to sun exposure. As shown in FIG. 1, when the changes of the sun exposed skin (A, B) and the unexposed skin (C, D) are observed, the skin exposed to the sun is thickened by the thickened skin , elastosis) can be observed to produce typical wrinkled, deep wrinkles and rough skin (A). In addition, skin is thinned due to decomposition of elastic fibers in dermis tissue (B). On the other hand, skin that is not exposed to the sun of a 45-year-old woman (C) and a 70-year-old woman (D) is thin, fine wrinkles, soft and dry with some degradation of elasticity, but has no defects such as spots and is pale and pale It can be confirmed that it has skin.

Intrinsic skin aging is predominantly genetically determined, clinically characterized by fragility and reduced elasticity of the skin and pale skin. In the case of endogenous aging, it is represented by gradual atrophy of the epidermis histologically, and the skin thickness may be thinned by 10 to 50% between 30 and 80 years of age.

The extracellular matrix (ECM) changes following endogenous aging are as follows. First, in the case of collagenous fibers, collagen fiber atrophy (especially reticular dermis) of the dermis is observed, and elasticity and tensile force may decrease due to an increase in cross-linking. Elastic fibers are composed of several components such as elastin, fibrillin-rich microfibrils, and microfibril associated glycoproteins (MAGPs), which form oxytalan fibers at the dermal-epidermal junction as the aging progresses Fibrillin-rich microfibrils Atrophy can be prominent. On the other hand, the extracellular matrix of the dermis is not only composed of fibrous proteins, but a variety of carbohydrates are present in the form of binding or independent of proteins. Such ECM components are mainly called glycosaminoglycans. Glycosaminoglycan is a general term for polysaccharides having hexoseamine and uronic acid or galactose disaccharide units as a basic structure. These hydrophilic glycoproteins and proteoglycans bind to abundant hyaluronic acid polymers, It is important to maintain the However, when skin aging occurs, glycosaminoglycan decreases and skin dryness may occur.

These processes of skin aging are: 1) weakening the proliferative potential of skin-derived cells (keratinocyte, fibroblast, melanocyte), 2) reducing the ability of the dermis to synthesize the matrix, 3) ) And increased expression of collagenase-related proteins for substrate degradation, including collagen components, and these phenomena can be compounded with each other and aging can proceed.

Although skin aging is known to be one of the major causes of collagen content, one of the main components of the skin, the precise mechanism for collagen destruction and cell proliferation and regenerative capacity is not yet fully understood. In addition, studies on the promotion of collagen synthesis and the development of collagen mass transfer materials to complement the increase in collagen breakdown rate have been actively carried out in the academia and the cosmetics industry, but there is no report showing that they have achieved remarkable results yet.

Therefore, at the present time, it is very important in the development of functional cosmetics for skin beauty, to find skin cell proliferation regulating materials capable of reducing skin aging speed, to find collagen degradation enzyme inhibiting materials and to promote collagen production. The purpose of this study was to investigate the effect of extracts composed of herbal medicines on the proliferation of skin cells, to confirm the inhibition of the activity and expression of collagenase expressed in keratinocytes, and to promote the collagen synthesis ability of collagen-producing fibroblasts I did a test to see if I was not. The results of these test examples are described in more detail below.

Extrinsic skin aging, on the other hand, is mainly affected by exposure to ultraviolet light, and it is generally known that over 80% of facial skin aging is affected by exposure to sunlight. Clinically, UV-damaged skin is known to have reduced elasticity, rough and dry skin, abnormal pigmentation and deep wrinkles. It is also observed that in the endogenous aging, the skin becomes thinner, whereas in the exogenous aging, the increase and decrease of the epidermal thickness (hyperplasia) and the severe atrophy occur together, and the epidermis loses its polarity . In addition, the distribution or function of the extracellular matrix previously described in the endogenous factor is significantly changed. Specifically, in the case of collagenous fiber, collagen reduction of net dermis is observed in endogenous aging, whereas collagen VII in fibrillar collagen and epidermal dermis junction region can be specifically reduced in exogenous . In the case of elastic fibers, the accumulation of abnormal elastic fibers by photoaging can occur throughout the dermis (solar elastosis), and thus the distribution of non-systematic elastic fibers can be observed. In the case of glycosaminoglycans (GAG), an abnormally formed glycosaminoglycan can accumulate in the skin.

The mechanism of exogenous aging can be confirmed by the mechanism of TRMP1-mediated MMP expression and activity regulation. Collagen is an important substance that constitutes extracellular matrix, which accounts for 80% of the total dry weight of the skin. It gives a strong tension to the dermis. Decrease of skin collagen content due to aging induces skin wrinkles, which is caused by both exogenous and endogenous aging Phenomenon. Collagen breakdown is partially associated with collagenase, a matrix metalloproteinase (MMP) that is formed in keratinocytes and fibroblasts. MMP is one of the matrix degrading enzymes and plays an important role in various destructive processes including inflammation. Several types of MMPs are associated with collagen destruction of the dermis due to exogenous and endogenous aging, which are usually expressed at low levels in the cells, but are expressed by various external stimuli such as ultraviolet light, infrared, growth regulators, and cytokines .

Figure 2 shows the remodeling of the extracellular matrix (ECM) of endogenous skin aging and exogenous skin aging. As shown in FIG. 2, remodeling of dermis collagen, elastic fibers, and glycosaminoglycans (indicated by red color) can be observed in the skin on which intrinsic and extrinsic aging proceed, You can observe something else. In Figure 2, Young refers to the inner skin of a forearm of a 23 year old, and Old represents a photoprotected and a photoexposed of a 75 year old, respectively.

Thus, exposure to ultraviolet light is known to activate the DNA binding protein AP1 (activator protein-1), thereby increasing the expression of MMP-1, 3, 9, and 12. In particular, collagen degradation by MMP- Once initiated, degraded collagen can be further degraded by MMP-3 and MMP-9.

This increase in MMP activity and expression is regulated by calcium. Increased extracellular calcium levels increase gene expression of MMP-9 in human keratinocytes. On the other hand, inhibition of intracellular calcium influx is known to decrease mRNA expression level of MMP-1. Recent studies have shown that intracellular calcium influx is caused by a non-selective cation pathway called TRPV1.

3 is a graph showing the effect of TRPV1 on the increase of MMP1 expression by ultraviolet exposure. As shown in FIG. 3, when ultraviolet irradiation activates TRPV1 through a PKC-dependent pathway, intracellular calcium influx occurs, which causes activation of MMP1 and degradation of collagen. UV irradiation also directly increases the amount of TRPV1 expression. Thus, it has recently been shown that the increase in the expression level of MMP-1 by UV exposure is caused by the PKC-dependent activation of TRPV1 and the resulting intracellular calcium influx, that is, the activation of MMP-1 by UV And by intracellular calcium influx by TRPV1.

These ultraviolet rays affect not only the activation but also the expression level of TRPV1. When the change in the expression level of TRPV1 after irradiating ultraviolet light to human skin tissue is examined, the amount of TRPV1 expression increases with time. Such an increase in the expression level of TRPV1 does not occur only in extrinsic aging but also in the expression of TRPV1 even in the case of intrinsic aging, that is, natural aging.

Therefore, the inventors of the present invention have found that when using the composition for skin aging, atopic amelioration, and skin whitening according to the embodiment of the present invention, activation of TRPV1, which is involved in an upper signal transduction mechanism, , And it was confirmed that the expression of MMP-1, a collagenase, which is a downstream signal thereof, can be inhibited. These test examples will be described later in more detail.

Mechanism of immunopathology of atopic dermatitis

Fig. 4 is a conceptual view showing the mechanism of immunopathology of atopic dermatitis. As shown in FIG. 4, when the allergen is recognized by IgE, it binds to the Rangelmann cell surface IgE-adherent Fc receptor and transmits the antigen to the T lymphocyte, thereby activating the T lymphocyte. Alternatively, T lymphocytes may be activated by superantigenic superantigen of self-peptide or staphylococci. Inflammatory cells infiltrating lesions of atopic dermatitis are mainly Th2 cells, which produce cytokines such as IL-4 and IL-5, which promote the rise of serum IgE and increase eosinophilia. On the other hand, Thl lumps are inhibited by TNF released from mast cells that are easily activated in atopic dermatitis, resulting in a decrease in cell mediated immunity. Various secreted cytokines activate vascular endothelial cells and induce or increase the expression of several cell adhesion molecules, thereby promoting the return of memory T lymphocytes and causing eczematous lesions.

The present invention relates to a composition for skin aging, atopic dermatitis, and skin whitening comprising a natural herbal extract, comprising extracts of extracts of Hashuo, mint, mashihyun, licorice, beeswax, Karendra, bamboo shoots, bamboo shoots, , Atopic dermatitis, and atopic dermatitis.

FIG. 5 is a view showing the composition of a composition for skin aging, atopic dermatitis, and skin whitening comprising natural herbal extracts according to an embodiment of the present invention. As shown in FIG. 5, the composition for skin aging, atopic dermatitis, and skin whitening comprising natural herbal extracts according to an embodiment of the present invention includes at least one selected from the group consisting of Hashuo, mint, mashihi, licorice, beeswax, , Horse mackerel, horsetail, saururus, and sulfur extract as an active ingredient. Preferably, the mixing ratio of the mint is 11 to 14 wt%, the mixing ratio of the mint is 11 to 14 wt%, the mixing ratio of the mint is 8 to 11 wt%, the mixing ratio of the licorice is 14 wt% To 17% by weight, the blending ratio of beeswax is 8% to 11% by weight, the blending ratio of calendra is 2% to 5% by weight, the blending ratio of bordering is 5% to 8% Is from 2% to 5% by weight, the mixture ratio of the mixture is from 8% to 11% by weight, the proportion of the perilla is from 0.1% to 3% by weight, the proportion of saururus is from 11% to 14% The blending ratio may be 5 wt% to 8 wt%. Preferably, the mixing ratio of the sewage is 12 wt% to 13 wt%, the mixing ratio of the pepper is 12 wt% to 13 wt%, the mixture ratio is 9 wt% to 10 wt%, the mixing ratio of licorice is 15 Wherein the blend ratio of beeswax is 9 wt% to 10 wt%, the blend ratio of calendula is 3 wt% to 4 wt%, the blend ratio of bordering is 6 wt% to 7 wt% The blend ratio of the syrups is 9 wt% to 10 wt%, the blend ratio of the syrups is 1 wt% to 2 wt%, the blend ratio of saururus is 12 wt% to 13 wt% , And the blending ratio of sulfur may be 6 wt% to 7 wt%.

Pleuropterus multiflorus TURCZ. Is a perennial herbaceous perennial herbaceous plant belonging to the genus Euphorbiaceae. It mainly uses root roots as a medicinal material. It is known to have warm, winding, and chopped have. In addition, it is known to have the efficacy of ginseng, blood, blood, interpreting, breeze, and slime. In the oriental medicine, it is used as a remedy for symptoms such as weakness of the body, back pain, arteriosclerosis, hypertension, chronic hepatitis, tuberculous lymphadenitis, enteritis and constipation Also.

Pepper (Mentha arvensis var. Piperascens) is a perennial perennial herbaceous perennial plant with monocotyledonous plants of the dicotyledonous plant. It has a rectangular cross-section with hairs on its surface. The main ingredient of peppermint oil, mint oil, is menthol, which can be used medicinally in coatings, painkillers, stimulants, insect repellents, or as a refreshing agent or fragrance for toothpaste, jam, candy, cosmetics, and tobacco.

Porthiaca oleracea L. is a prototype of carapace with leggings. It has a leaf shape similar to that of a horse. It has fever, detoxification, and hemostatic effects. It has bacterial dysentery, swelling, hemorrhoids, cervical lymphadenitis, eczema, , Uterine bleeding, urinary discomfort, and the like. It is known that the antimicrobial action by the pharmacological action, the intestinal interlocking action by the enhancement of the uterine smooth muscle contraction, and the diuretic action are known.

Licorice (Glycyrrhiza uralensis Fischer) has a unique odor and sweet taste, which is effective against detoxification, hepatitis, urticaria, dermatitis and eczema. It has the effects of Jinhae, Gadam, muscle relaxation, diuretic action, anti-inflammatory action and suppression of peptic ulcer It is known.

Beeswax (beeswax) is an animal solid wax extracted from a honeycomb by heat pressing, solvent extraction, etc. It contains palmitic acid ester of melissyl alcohol and chitosan as main components, and also contains various fatty acids, alcohols, hydrocarbons Lt; / RTI >

Calendula officinalis (Calendula officinalis) is a chrysanthemum of the dicotyledonous plant. It is a first year or a perennial plant. The pesticide using the flower is an excellent first aid treatment where burns, bubbles, It is known to be effective as cleansing water. It is also used as an adjuvant for the treatment of candidiasis. It is also known to be effective for gastritis, gastric ulcer, and duodenal ulcer.

Poria is a kind of sclerotia that grows on the root of pine trees that has been bred for many years. It is said to have a function of taste, boredness and quality, and it is used as a tonic It is also used for recovery after illness, and it is known that it has a medicinal effect such as diuretic action, lowering of blood sugar level, and soothing action.

Bombycis corpus is known to dry scales of the silkworm moth, which are killed by powdery mildew, to relieve the wind and to fight off the disease. Symptoms of epileptic seizures include scorpion, . It is known that when the mouth is turned by a paralytic or when facial muscles are contracted, they are used together with scorpions and white believers, and when headache is caused by fever and liver fever, blinking of the eyes and sore throat or sore.

Equisetum arvense is a perennial herbaceous plant of the genus Vanderbilt, which feeds well, can be used for edible reproduction, or can be used as a diuretic.

Houttuynia cordata (Houttuynia cordata) is a perennial plant of the dicotyledonous plant Peppercorn Saururus chinensis, which is also known as anilinopsis, as a diuretic and insecticide prior to flowering, and it is applied when the leaves are bitten and swollen and poisonous. It is used for swelling, pneumonia, hemorrhoids, and it is known that plants are used for gonorrhea, enteritis, urinary tract infection, pneumonia, bronchitis in one room.

Saururus chinensis (Lour.) Baill. Is a perennial plant whose roots, leaves and flowers are white. It can be used as an antidote to all plants or as a diuretic. In one room, the whole plant is dried and swollen, and it is used when urine does not come out well, or it is known to be used also for jaundice and hepatitis.

Sulfur (sulfur), which can be used to treat hemorrhoids and hemorrhoids, is known to strengthen muscles and skeletal, cure baldness, and kill insect-causing worms. In addition, sulfur has a lowering effect, and for a long time it is known that the back and the kidney treat the cold symptoms and treat the symptoms and the haemorrhagic fever due to the fragility of the wind.

The composition for skin aging, atopic dermatitis, and skin whitening comprising natural herbal extracts according to one embodiment of the present invention is characterized by comprising at least one component selected from the group consisting of 1) horseradish, mint, mashii, licorice, beeswax, karendela, balsam, Dried and pulverized, and 3) powdered sewage, peppermint, beeswax, Karendra, bamboo shoots, bamboo shoots, bamboo shoots, Can be prepared by extracting onion with water, and 4) can be prepared by extracting onion with mushroom, licorice, beeswax, Karendra, white bamboo, white bamboo, bamboo shoot, , Bombyx mori, Rhizoctonia sp., Rhododendron syrup, Saururus chinensis, and Sulfur freeze-dried to completely dry to a moisture content of less than about 10% by weight.

In the following description and drawings, the composition for skin aging, atopic dermatitis, and skin whitening comprising natural herbal extracts according to one embodiment of the present invention is represented by SB.

Test Example Antioxidant activity evaluation 1 (DDPH radical scavenging effect)

In order to confirm the DPPH radical scavenging activity (DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity), the present inventors conducted a test according to the method of Blosi. That is, 4 ml of DPPH radical 1.5 × 10 ^-5 M ethanol solution was added to 0.1 ml of each sample at 1.0 mg / ml, 2.0 mg / ml and 4.0 mg / ml, reacted at room temperature for 30 minutes, And the effect was calculated by the following formula 1 in%.

[Formula 1]

Radical scavenging activity (%) = {(ODcontrol - ODsample) / ODcontrol} x 100

DPPH is a relatively stable radical, unlike unstable radicals such as hydroxyl radical (· OH) or superoxide anion (· O ₂ ^- ).

FIG. 6 is a graph showing the results of a test example of DPPH radical scavenging activity of a composition for skin aging, atopic dermatitis, and skin whitening comprising a natural herbal extract according to an embodiment of the present invention. As shown in FIG. 6, the test result showed that the composition (SB) according to one embodiment of the present invention had an excellent radical scavenging activity of about 70.2%, and the positive control ascorbic acid had a scavenging activity of about 95% Respectively.

Test Example Antioxidant Activity Evaluation 2 (Lipid Peroxidation Inhibitory Effect)

The present inventors measured lipid peroxidation inhibitory effect as another index of antioxidant activity. Anti-lipid peroxidation activity was calculated by measuring the amount of lipid peroxide according to Ohkawa's method. First, 0.65 ml of 0.1 M potassium phosphate buffer (pH 7.4), 0.05 ml of sample and 0.1 ml of homogenate were added and incubated at 37 ° C for 1 hour. After 1 hour, 0.2 ml of 8.1% SDS and 0.1 ml of acetate buffer (pH 3.5) and 1.5 ml of 0.8% TBA were added, reacted at 95 ° C for 1 hour, and then cooled to room temperature. 5 ml of n-butanol: pyridine (v / v) was added to the reaction mixture, and the mixture was centrifuged at 3,000 rpm for 10 minutes. Absorbance was measured at 532 nm using supernatant. The compound was dissolved in DMSO and α-tocopherol was used as a positive control. The inhibition rate (%) was calculated according to the following [Equation 2] using a solution prepared by adding DMSO instead of the sample as a control.

[Formula 2]

Inhibition (%) = {(O.Dcontrol- O.Dsample) / O.Dcontrol} 100

FIG. 7 is a graph showing the results of a test example of skin aging, atopic improvement, and lipid peroxidation inhibitory activity of a composition for skin whitening comprising a natural herbal extract according to an embodiment of the present invention. As shown in FIG. 7, the unsaturated fatty acid of the phospholipid constituting the cell membrane causes the lipid peroxidation reaction by the active oxygen, and the lipid peroxidation produced is the main cause of the cell membrane damage including the neuronal cell, And the lipid peroxidation content of the sample was measured in vitro. As a result, it was confirmed that the composition (SB) according to one embodiment of the present invention showed an activity of inhibiting lipid peroxide formation of 20.6%.

Test example Antioxidant activity evaluation 3 (peroxide anion scavenging activity)

The present inventors measured NBT reduction method to measure the superoxide anion elimination effect as another index of antioxidant activity. Superoxide anion causes lipid peroxidation by producing more dangerous reactive oxygen species such as singlet oxygen and hydroxyl radical by many enzymatic systems. Superoxide anion activates free radicals that can react with biological macromolecules, which can cause tissue damage And may initiate lipid peroxidation. Thus, peroxide anion radicals are normally produced at first, but their effects are magnified by creating other free radicals and oxidants.

Superoxide anion scavenging activity was measured by Gotoh and Niki method. First, 100 μl of 30 mM EDTA (pH 7.4), 10 μl of 30 mM hypoxanthine and 200 μl of 1.42 mM NBT were added to 30 μl of a sample at a concentration of 1.0 mg / ml, 2.0 mg / ml and 4.0 mg / ml, After 3 minutes of reaction, 100 μl of 0.5 U / ml xanthine oxidase was added and the total volume was adjusted to 3 ml with 50 mM phosphate buffer (pH 7.4). The reaction solution was allowed to react at room temperature for 20 minutes, and the absorbance was measured at a wavelength of 560 nm. As a positive control, ascorbic acid was used, and the inhibition rate (%) was calculated according to the following [Equation 3] using a solution in which DMSO was added instead of the sample as a control.

[Formula 3]

Inhibition ratio (%) = {1 - (O.Dsample / O.Dcontrol)} 100

FIG. 8 is a graph showing the results of a test example of skin aging, atopic improvement, and peroxide anion scavenging activity of a composition for skin whitening comprising a natural herbal extract according to an embodiment of the present invention. As shown in FIG. 8, it was confirmed that the composition (SB) according to one embodiment of the present invention exhibits a superoxide anion scavenging activity of 15.8%.

Test Example Evaluation of skin aging inhibiting activity

The present inventors evaluated the elastase inhibitory activity. In the enzyme reaction used for evaluation of skin aging inhibition activity, 1300 μl of a substrate solution to which N-succinyl-Ala-Ala-Ala-p-nitroanilide (1 mM) was added to Tris-Cl (0.12M pH 8.0) Was dissolved in 1 ml of DMSO, and 7.5 占 퐇 of the solution was used. Kojic acid was used in 7.5 ㎕ of 200 ㎍ / ml solution. Then, 92.5 ㎕ of Tris-Cl (0.12 M, pH 8.0) solution was added and preincubation was performed for 10 minutes. 100 ㎕ of enzyme (Elastase 0.0375 U / ml) was added and incubated for 10 minutes and absorbance was measured at 410 nm.

FIG. 9 is a graph showing the results of a test example of skin aging, atopic improvement, and skin whitening inhibitory activity of natural herb medicine extract according to an embodiment of the present invention. Elastase is an enzyme that degrades elastin. When it is excessively secreted, elastin of the skin is decomposed to lose elasticity of the skin. As a result of measuring the activity, the composition according to one embodiment of the present invention SB) showed an elastase inhibitory activity of 40.74%.

Test Example Skin whitening effect evaluation

The present inventors evaluated the tyrosinase inhibitory activity. For the enzyme reaction used in the skin whitening effect evaluation, 1.8 ml of sodium phosphate buffer (1/15 M pH 6.8), 1.0 ml of 1.5 mM L-DOPA, and 0.1 ml of a 10 mg sample dissolved in 1 ml of DMSO were used. Kojic acid (positive control drug) was used in 0.1 ml of 200 ㎍ / ml solution. In addition, 0.1 ml of Enzyme (Mushroom tyrosinase 1000 U / ml) solution was added, incubated for 10 minutes, and the enzyme activity was stopped in an ice bath. Absorbance was measured at a wavelength of 475 nm.

10 is a graph showing the results of a test example of skin aging, atopic improvement, and tyrosinase inhibitory activity of a composition for skin whitening comprising a natural herbal extract according to an embodiment of the present invention. Tyrosinase is an enzyme that acts to form melanin from L-DOPA. L-DOPA inhibits the production of melanin from the substrate. As a result of the activity measurement, It was confirmed that the composition (SB) according to one embodiment showed a tyrosinase inhibitory activity of 25.80%.

Test Example Cytotoxicity Assay

The present inventors evaluated the antiinflammatory effect and cytotoxicity of skin aging, atopic improvement and skin whitening compositions including natural herbal extracts. For this purpose, the cells were cultured in a medium containing 10% heat-inactivated FBS, 100 units / ml penicillin, 100 ng / ml streptomycin, and mouse macrophage RAW 264.7 cells (ATCC; Manassas, Va. The cells were cultured in DMEM medium at 37 ° C and 5% CO 2 and assayed for cytotoxicity (MTT assay). This method measures the conversion of MTT to formazan by mitochondrial dehydrogenases. To do this, 100 μl of RAW 264.7 cells (5 × 10 5 cells / ml) were seeded in 96-μl titer well plate, cultured for 24 hours, and cultured for 24 hours at various concentrations. After 24 hours, 20 μl of 5 mg / ml MTT solution was added to each well and incubated for 4 hours. Then, the culture solution was removed and the resulting formazan was dissolved in 100 μl of DMSO. Then, 900 μl of water was mixed with UV-VIS spectrophotometer The absorbance was measured at a wavelength of 450 nm. The relative cell viability (%) of the sample treated group was calculated with the 100% survival rate of the untreated control (control) cultured only in the cytotoxicity.

FIG. 11 is a graph showing the results of a cytotoxicity test example of skin aging, atopic improvement, and skin whitening composition containing a natural herbal extract according to an embodiment of the present invention. As shown in FIG. 11, the present inventors conducted an MTS assay to examine the cytotoxicity of the composition (SB) according to one embodiment of the present invention to mouse RAW 264.7 cells, which are macrophages. When the samples of the composition (SB) were treated for 24 hours at concentrations of 7.5, 15, 30, 60, 120 and 240 / / ml, no toxicity was observed at 120 / / ml, Cytotoxicity was observed. Therefore, it was confirmed that the sample was safe for cytotoxicity at a concentration of less than 120 ㎍ / ml.

Test Example Evaluation of skin ion channel control efficacy

FIG. 12 is a view showing a configuration of a device for evaluating skin ion passage control efficacy of a skin aging, atopic improvement and skin whitening composition containing a natural herbal extract according to an embodiment of the present invention. As shown in FIG. 12, the inventors of the present invention have found that, for skin aging, improvement of atopy, and evaluation of skin ion channel control efficacy of a composition for skin whitening comprising natural herbal extracts according to an embodiment of the present invention, patch patches clamp was used to evaluate skin ion channel control efficacy. The patch clamp is a device that allows the glass microelectrode to adhere to the cell and measure the current flowing in and out of the cell. HEK293 cells overexpressing TRPV1 and Orai1 ion channel proteins were subjected to conventional whole cell patch clamp using a patch clamp. The glass electrode had a resistance of 2 ~ 4 MW during the whole cell patch clamp, resistance during inside out and cell attached patch clamp > 1.2 MW was used. The overall signal from a patch clamp amplifier (Axopatch 1-D, Axon instruments, USA) was digitized and stored via Axoscope 9.0 (Axon instruments, USA). To record and store the current response at fixed voltage and excitation voltages, a pClamp software (v9.2) and an analog-to-digital converter (Digidata-1322A, Axon instruments, USA ) Were used. The results obtained in the membrane voltage clamp test were analyzed and processed using clampfit v9.2, origin (Microcal software, USA).

FIG. 13 is a graph showing the skin aging, atopic improvement, and skin whitening composition inhibiting effect of TRPV1 ion channel, which comprises a natural herbal medicine extract according to an embodiment of the present invention. As shown in FIG. 13, it was confirmed that the composition (SB) sample according to one embodiment of the present invention does not effectively inhibit the passage of TRPV1 ion causing skin aging. However, the inhibition effect of elastase was about 40% at the sample concentration of 100 mg / ml. It is considered that the skin aging inhibition effect such as wrinkles of this sample is due to the enzyme inhibition which causes decomposition of elastin, not TRPV1 ion channel inhibition.

FIG. 14 is a graph showing the skin aging, atopic improvement, and skin whitening compositions containing the natural herbal extract according to an embodiment of the present invention. As shown in FIG. 14, the composition (SB) sample according to one embodiment of the present invention showed some inhibition of the Orai 1 ion passage associated with skin whitening. In particular, it has been confirmed that the composition (SB) according to one embodiment of the present invention has a tyrosinase inhibitory activity of about 28%, which is a cause of darkening skin.

Test Example Nitrogen Oxide Measurement

The present inventors measured the amount of nitric oxide (NO), which is an active nitrogen species produced in cells cultured in the same manner as the MTT assay, in order to measure NO production amount as an inflammatory mediator, . Specifically, samples were treated at various concentrations after culturing for 24 hours, and LPS was treated to a final concentration of 200 ng / ml 30 minutes after the sample treatment. After 24 hours of incubation, RAW 264.7 cells were irritated. To confirm the amount of NO produced, the culture solution was collected, and 100 μl of the culture solution and 100 μl of Griess reagent (0.1% N-NED, 1% sulphanilamide 1% H 3 PO 4 solution) And then mixed with 800 μl of water. The absorbance at 570 nm was measured using a UV-VIS spectrophotometer. The concentration in the cell culture medium was compared with the relative amount (% of control) of the sample treated group, with the amount of NO produced in the group treated with LPS alone as 100%, compared with the amount of NO produced in the normal group without LPS treatment.

FIG. 15 is a graph showing the skin aging, atopic improvement, and inhibition of NO production of a skin whitening composition containing a natural herbal extract according to an embodiment of the present invention. As shown in FIG. 15, in the RAW 264.7 macrophage induced by LPS treatment, the effect of the sample on the production of NO, which is an inflammation mediator, after treatment of a concentration not showing cytotoxicity was examined. As a result, The composition according to the Example (SB) inhibited the production of NO, an inflammatory mediator, at a concentration of 11.77 μM.

Test example PGE2 measurement

To measure the amount of Prostaglandin E2 (PGE2) produced by the inflammation mediator, RAW 264.7 cells were pretreated with each sample (100 μg / mL) for 1 hour, and the cells were treated with LPS for 12 hours. The PGE2 level in the C fluorescence media was analyzed by the kit method using the PGE2 detection kit.

FIG. 16 is a graph showing the skin aging, atopic improvement, and PGE2 inhibitory effect of a skin whitening composition containing a natural herbal extract according to an embodiment of the present invention. As shown in FIG. 16, when RAW 264.7 macrophage induced inflammation by LPS treatment, the sample was treated at a concentration not showing cytotoxicity, and then the effect on the amount of PGE2, an inflammation mediator, was examined. As a result, It was confirmed that the composition (SB) according to the Example inhibits the production of PGE2, an inflammation mediator.

Test Example Pro-inflammatory cytokine assay

The present inventors measured the amounts of IL-1β, TNF-α and IL-6, which are inflammatory mediators, respectively. For this purpose, ELISA assay kit was used to analyze the effect of each sample on cytokines (IL-1β, IL-6, and TNF-α) produced by LPS-stimulated RAW 264.7 cells. Each sample (100 ㎍ / mL) and RAW 264.7 cells were preincubated for 1 hour and then treated with LPS for 12 more hours. The supernatant was removed and IL-1β, TNF-α, and IL-6 levels were analyzed by ELISA kit. The concentration of cytokine was measured using a standard curve.

FIG. 17 is a graph showing the skin aging, atopic improvement, and IL-1? Inhibiting effect of a skin whitening composition containing a natural herbal medicine extract according to an embodiment of the present invention. As shown in FIG. 17, in the RAW 264.7 macrophage in which inflammation was induced by LPS treatment, the effect of the sample on the production of IL-1β, which is an inflammation mediator, after treatment with a cytotoxic concentration was examined. As a result, The composition (SB) according to one embodiment was found to inhibit the production of inflammatory mediator IL-1?.

FIG. 18 is a graph showing the skin aging, atopic improvement, and skin whitening composition TNF-.alpha. Inhibitory effect including natural herbal extracts according to an embodiment of the present invention. As shown in FIG. 18, in the RAW 264.7 macrophage induced by LPS treatment, the effect of the sample on the amount of TNF-α, which is an inflammation mediator, after treatment with a cytotoxic concentration was examined. As a result, The composition (SB) according to one embodiment was found to slightly inhibit the production of TNF-a, an inflammation mediator.

19 is a graph showing the skin aging, atopic improvement, and IL-6 inhibitory effect of a composition for skin whitening comprising a natural herbal extract according to an embodiment of the present invention. As shown in FIG. 19, in the RAW 264.7 macrophage in which inflammation was induced by LPS treatment, the sample was treated at a concentration that did not show cytotoxicity, and then the effect on the amount of IL-6 produced as an inflammation mediator was examined. (SB) according to one embodiment of the present invention did not inhibit the production of inflammatory mediator IL-6.

Clinical trial example

20 to 23 show results of clinical application of skin aging, atopic improvement and skin whitening composition comprising natural herbal extracts according to an embodiment of the present invention to skin troubles. As shown in FIGS. 20 to 23, when the composition (SB) according to one embodiment of the present invention was applied to atopic skin troubles, it was confirmed that it was effective in treating or improving atopic skin inflammation as well as improving wrinkles there was.

The present invention may be embodied in many other specific forms without departing from the spirit or essential characteristics of the invention.

Claims (8)

As a composition for skin aging, atopic improvement and skin whitening,
The present invention relates to a method for producing an extract of the present invention, which comprises extracting an extract of Sasa, Mint, Mashin, licorice, beeswax, Karendra,
The blending ratio of the mint is from 11 to 14 wt%, the blending ratio of the mint is 11 to 14 wt%, the blending ratio of the mint is 8 to 11 wt%, the blending ratio of licorice is 14 to 17 wt% By weight, the blending ratio of beeswax is 8 to 11% by weight, the blending ratio of calendra is 2 to 5% by weight, the blending ratio of balsam is 5 to 8% by weight, % Of the saururus, 11 to 14 wt.% Of the saururus, and 3 to 5 wt.% Of the saururus, the mixture ratio of the saurus is 8 to 11 wt.%, A composition for skin aging, atopic dermatitis and skin whitening comprising a natural herbal extract, wherein the compounding ratio is 5 wt% to 8 wt%.
delete The method according to claim 1,
Wherein the mixing ratio of the mint is 12 wt% to 13 wt%, the mixing ratio of the mint is 12 wt% to 13 wt%, the mixing ratio of the mint is 9 wt% to 10 wt%, the mixing ratio of the licorice is 15 wt% To 16% by weight, the mixing ratio of the beeswax is 9% by weight to 10% by weight, the mixing ratio of the Karendra is 3% to 4% by weight, the mixing ratio of the beeswax is 6% Wherein the mixing ratio of the bamboo shoots is 3 wt% to 4 wt%, the mixture ratio of the bamboo shoots is 9 wt% to 10 wt%, the mixing ratio of the perch is 1 wt% to 2 wt% To 13% by weight, and the blending ratio of sulfur is from 6% by weight to 7% by weight. The composition for skin aging, atopic dermatitis, and skin whitening comprises natural herbal extracts.
delete delete delete A method for producing a composition for skin aging, atopic improvement and skin whitening,
(1) Preparing Sasa, Mint, Mashin, Licorice, Beeswax, Karendra, Baekbyeong, Baekjangjae, Rhizoma, Rhizoma, Saururus and Sulfur;
(2) drying and pulverizing the Hashuo, mint, mashin, licorice, beeswax, Karendra, white bamboo, white bamboo, white bamboo, horse mackerel, saururus and Sulfur; And
(3) a step of extracting the natural herb medicine extract, which comprises the step of extracting the powdered Sasao, mint, mashin, licorice, beeswax, Karendra, white bream, white bamboo shoot, horse mackerel, However,
(4) The method of the present invention further comprises freeze-drying the extracts so that the moisture content of the extracted Husso, mint, mashin, licorice, beeswax, Karendra, bamboo shoots, bamboo shoots, A method for the preparation of a composition for skin aging, improvement of atopy and skin whitening comprising natural herbal extracts. delete

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