KR101750909B1 - Water-soluble cutting oil comprising culture medium of photosynthetic bacteria - Google Patents

Abstract

The present invention relates to an anti-corrosive agent including a photosynthetic bacterial culture solution and an aqueous cutting oil with improved perishability. The water-soluble cutting oil including the photosynthetic bacterial culture liquid is not only excellent in internal perishability but also improves rustproofing property without containing a preservative and can be usefully used in metal processing.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a water-soluble cutting oil containing a photosynthetic bacterial culture liquid,

The present invention relates to a water-soluble cutting oil having enhanced rustproofing property and corrosion resistance including a photosynthetic bacterial culture liquid.

Processing for cutting and grinding iron, aluminum, various metals, glass, ceramics and the like is one of the processing operations for removing unnecessary portions from the workpiece by the cutting tool. Through this machining operation, the workpiece can be machined into a desired shape, dimension, or surface. A large friction occurs at the contact surface between the workpiece and the tool in the course of the cutting process, the tool or the workpiece is damaged by the heat generated thereby, or the machining is not performed as desired. In order to solve this problem, cutting oil is used in the cutting process. The cutting oil reduces the friction between the workpiece and the tool to impart lubricity. Further, the cutting oil improves the cooling performance of the metal workpiece and prolongs the tool life. These cutting oils include water-soluble and non-water-soluble cutting oils.

Non-water soluble cutting oil is an emulsion containing sulfur or chlorine based on mineral oil. However, such non-water soluble cutting oil has a risk of ignition if the temperature of the emulsion increases during the cutting process. In addition, the scattering of the emulsion particles due to high-speed rotation causes contamination of the surrounding environment, and the floor of the factory slips, increasing the risk of safety accidents. Further, there is a problem that the harmfulness of the human body is increased. Therefore, non-water-soluble cutting oil is mainly used when a portion to be cut is large, and water-soluble cutting oil is mostly used.

Water soluble cutting oil includes raw materials such as lubricating oil, lubricant, extreme pressure additive, fatty acid, rust inhibitor additive, emulsifier, preservative. The water-soluble cutting oil is used by being diluted with water. As a result, the rustproofing performance may be lowered due to high frictional heat involved in the cutting operation, incorporation of a metal chip, oxidation and aging of the emulsion. Accordingly, rusting of the processed metal or discoloration or corrosion of the non-ferrous metal may proceed. Also, due to the microbial spoilage, odor is generated in the cutting oil, which frequently causes economic problems due to frequent exchange of emulsions and addition of additives.

In general, substances with bactericidal activity or fungicide activity are added as preservatives to prevent decay of cutting oil. Examples thereof include formalin or a derivative thereof, BIT, MIT, IPBC, sodium 2-pyridinethiol-1-oxide and C ₅ H ₄ NOSNa. In Korean Patent No. 0134087, a water-soluble cutting oil having excellent antimicrobial activity was prepared by adding a compound of amines to prevent decay of water-soluble cutting oil. However, such a preservative has a problem indicating toxicity to human body.

Accordingly, research has been conducted on the development of a cutting oil composition for preventing corrosion on metal, preventing decay of cutting oil, and reducing harmfulness to human body, but this is a trivial matter.

The present inventors have produced a water-soluble cutting oil containing a photosynthetic microorganism and a group of useful microorganisms (EM) and having oxidative stability and endosperity.

Korea Patent No. 0134087

It is an object of the present invention to provide a water-soluble cutting oil containing a photosynthetic bacterial culture liquid in place of a preservative harmful to human body.

Another object of the present invention is to provide a process for producing the water-soluble cutting oil.

In order to achieve the above object, the present invention provides a cutting oil including a photosynthetic bacterial culture liquid.

According to another aspect of the present invention, there is provided a method for culturing photosynthetic bacteria, Mixing the cultured photosynthetic bacteria with EM to secondary culture; And mixing the secondary culture liquid with a water-soluble cutting oil, wherein the rust-inhibiting effect is enhanced.

The water-soluble cutting oil containing the photosynthetic bacterial culture liquid of the present invention is not only excellent in internal perishability but also improves rustproofing property without containing a preservative and can be usefully used in metal processing.

Fig. 1 is a photograph taken immediately after placing a nail and a metal chip in a water-soluble cutting oil in order to compare the rust prevention effect. Specifically, as the water-soluble cutting oil, commercially available water-soluble cutting oil of No. 1 in No. 1 and water-soluble cutting oil of the present invention were added to the photosynthetic bacterial culture solution (BIOFM) of the present invention or commercially available photosynthetic bacteria (A-type photosynthetic microorganism: AquaReader PSB Ltd.) and D Company EM fermentation broth: EM-K (EM Farm, Korea); B photosynthetic microorganism: Jell Na photosynthetic microorganism Probiotics , Korea)) was used.
FIG. 2 is a photograph showing the results of comparing the rust inhibition effect of the photosynthetic bacterial culture liquid of the present invention or commercially available photosynthetic bacteria added to the water-soluble cutting oil. Specifically, commercially available photosynthetic bacteria were added to commercially available water-soluble cutting oil and compared with the water-soluble cutting oil to which the photosynthetic bacterial culture solution of the present invention was added at the time of rusting:
A: Comparison of photosynthetic bacteria of Harvey World with photosynthetic bacterial culture of the present invention (photographed after 4 hours);
B: Comparison between photosynthetic bacteria of a biotechnology company and photosynthetic bacteria culture of the present invention (photographed after 6 hours);
C: Comparison of the EM fermentation broth of Comem Biotech with the photosynthetic bacterial culture of the present invention (photographed after 1 day);
D: Comparison of EM fermentation broth of Emm Farm with photosynthetic bacterial culture of the present invention (photographed after 1 day);
E: Comparison of photosynthetic microorganism primary cultured with photosynthetic bacterial culture of the present invention (photographed after 6 hours); And
F: Comparison between the negative control and the photosynthetic bacterial culture of the present invention (photographed after one day).
Fig. 3 is a photograph taken immediately after placing a nail and a metal chip in a water-soluble cutting oil. The rust inhibitive effect was compared when the photosynthetic bacterial culture liquid of the present invention or commercially available photosynthetic bacteria were added to commercially available water-soluble cutting oil No. 1 of No. 1 and the above water-soluble cutting oil.
Fig. 4 is a photograph showing the anti-rust effect of the photosynthetic bacterial culture solution of the present invention or commercially available photosynthetic bacteria. Specifically, commercially available photosynthetic bacteria were added to a commercially available water-soluble cutting oil and compared with the water-soluble cutting oil to which the photosynthetic bacterial culture solution of the present invention was added at the time of rusting:
A: Comparison of photosynthetic bacteria of Harvey World with photosynthetic bacterial culture of the present invention (photographed after 2 hours);
B: Comparison between the photosynthetic bacteria of one bio company and the photosynthetic bacterial culture of the present invention (photographed after one hour);
C: Comparison between the EM fermentation broth of Konem Biotech and the photosynthetic bacterial culture of the present invention (photographed after 27 hours);
D: Comparison of the EM fermentation broth of EM Farm with the photosynthetic bacterial culture of the present invention (photographed after 20 hours);
E: Comparison of photosynthetic microorganism primary cultured with photosynthetic bacterial culture of the present invention (photographed after 2 hours); And
F: Comparison between the negative control and the photosynthetic bacterial culture of the present invention (photographed after one day).
Fig. 5 is a photograph taken immediately after putting an iron nail and a cast iron casting chip into a water-soluble cutting oil to which preservatives and rust preventives are not added.
6 is a photograph (S-1: Comparative Example 9; S-2: Comparative Example 10; S-3: Comparative Example 11; S-4 : Comparative Example 12; S-5: Example 19; S-6: Example 20; and S-7: Example 21).

Hereinafter, the present invention will be described in detail.

The present invention provides a cutting oil containing a photosynthetic bacterial culture liquid.

The term " photosynthetic bacteria "as used herein refers to bacteria that grow using light energy. The photosynthetic bacteria include photosynthetic prokaryotes, such as turmeric, prochloron, and photosynthetic bacteria. Specifically, according to one embodiment, the photosynthetic bacteria may be purplish non-sulfur bacteria.

The purple non-sulfur bacteria use mainly low-molecular organic compounds such as malic acid or lactic acid or H ₂ as purplish electronic materials in purple bacteria. The bacterium is a photosynthetic bacterium capable of reducing and assimilating CO ₂ and growing, and belongs to Rhodospirillaceae. According to an embodiment of the present invention, the purple non-sulfur bacteria may be Rhodobacter sp., Rhodobacter sphaeroides .

The Rhodobacter spp. Is a gram-negative bacterium and is a tuberous anaerobic bacterium.

As used herein, the term "photosynthetic bacterial culture fluid" means a culture of the above-described photosynthetic bacteria alone or in combination with a useful microorganism group (EM). The photosynthetic bacterial culture solution may be a culture solution obtained by culturing the photosynthetic bacteria alone; A culture solution obtained by mixing at least one microorganism selected from lactic acid bacteria, yeast and Bacillus subtilis with photosynthetic bacteria; Or a culture obtained by mixing and culturing a useful microorganism group (EM) and photosynthetic bacteria.

As used herein, the term "effective microorganisms (EM)" is a combination of dozens of microorganisms beneficial to humans among many microorganisms present in nature. Photosynthetic bacteria, lactic acid bacteria, yeast, Bacillus subtilis, and actinomycetes are the major organisms constituting the EM. The fermentation products produced by the complex coexistence relationship of these bacteria exhibit the effect of EM. Specifically, the EM may include photosynthetic bacteria, lactic acid bacteria, and yeast. It may further contain Bacillus subtilis. These useful microorganisms are classified into 'EM-1', 'EM-5', 'EM-X', 'EM-Z', 'EM Bokashi', 'EM Ceramics', 'EM FPE', 'Activated EM' It is commercially available under the same brand name and is known to anyone so that it can be easily purchased and used.

The lactic acid bacteria are bacteria that decompose saccharides such as glucose to produce lactic acid. Lactic acid bacteria produce lactic acid by lactic acid fermentation, and are used in the manufacture of foods such as dairy products, kimchi, and brewed foods. It also plays a role in preventing abnormal fermentation by germs inhabiting mammalian gut. Gram-positive bacteria, tuberous anaerobic bacteria or anaerobic bacteria. Lactobacillus is known to be a variety of Lactobacillus and Streptococcus spp.

The yeast is a microorganism used for making bread, beer, wine, and the like, and is a collective term for single celled organisms that do not have photosynthetic ability or motility. Yeast can dissolve monosaccharides, such as hexoses, through inorganic respiration, without the supply of oxygen, to produce energy, which in turn releases alcohol and carbon dioxide. The yeast can grow under temperature conditions of 10 占 폚 to 37 占 폚.

The Bacillus subtilis is a non-pathogenic bacterium widely distributed in nature, particularly in air, dry grass, sewage and soil. It is a rod-like bacterium, which is acted upon by a flagella, and forms round or ovoid spores at the center of the cells. Bacillus subtilis is a gram-positive bacterium containing glycogen, and it decomposes a large number of carbohydrates to produce an acid. It also grows well under the temperature condition of 30 캜 to 70 캜.

Actinomycetes are microorganisms that exist as mycelium and sporophyll in soil, plant, animal, river, and seawater, and are classified into prokaryotes close to bacteria, that is, actinomycetes of bacteria. The cell size is similar to that of bacteria, and the cells grow like a mycelium of fungus in a threadlike shape and form spores at the end. Actinic actinomycetes play an important role in decomposition of various organic matter, especially degradation of organic matter, and also produce antibiotics.

The concentration of photosynthetic bacteria contained in the EM according to the invention may be 1.0x10 ⁴ to 1.0x10 ⁶ cfu / g, 1.0x10 ⁵ to 9.0x10 ⁵ cfu / g, or 2.0x10 ⁵ to 3.5x10 ⁵ cfu / g. On the other hand, the concentration of lactic acid may be 1.0x10 ⁵ to 1.0x10 ¹⁰ cfu / g, 1.0x10 ⁶ to 5.0x10 ⁹ cfu / g or 3.0x10 ⁶ to 2.0x10 ⁹ cfu / g. In addition, the concentration of the yeast may be 1.0x10 ⁴ to 1.0x10 ⁸ cfu / g, 1.0x10 ⁵ to 5.0x10 ⁷ cfu / g or 3.0x10 ⁵ to 3.0x10 ⁷ cfu / g. In addition, the concentration of Bacillus subtilis contained in the EM may be 1.0x10 ³ to 1.0x10 ⁵ cfu / g, 1.0x10 ⁴ to 5.0x10 ⁴ cfu / g or 3.0x10 ⁴ to 4.0x10 ⁴ cfu / g. In one embodiment of the invention, the concentration of the photosynthetic bacteria, lactobacillus, yeasts, and Bacillus subtilis of about 3.1x10 ⁵ cfu / g, about 1.9x10 ⁹ cfu / g, approximately 2.8x10 ⁷ cfu / g and about 3.1x10 ^4, respectively cfu / g

The culture solution according to the present invention can be obtained by mixing and culturing photosynthetic bacteria using a medium selected from the group consisting of molasses, wheat bran, rice bran, and a mixture thereof, and molasses can be used specifically.

As used herein, the term " molasses "or" molasses active liquid "is used interchangeably, meaning photosynthetic bacteria and EM that are used as food during fermentation and are essential components for the activation of bacteria. Wherein the molasses is selected from the group consisting of 1-3% (w / w) potassium, 1.5-3% (w / w) magnesium, 0.1-1% (w / w) calcium, 15-35% (w / And 45 to 55% (w / w) sugar. According to one embodiment of the present invention, the molasses comprises 1.85% (w / w) potassium, 2.24% (w / w) magnesium, 0.79% (w / w) calcium, 20-30% ) Of water and 50 to 60% (w / w) sugar.

The medium may further comprise an antioxidant. Any antioxidant may be used as long as it is a substance known to have an antioxidative effect. Specifically, the antioxidant may be any one selected from the group consisting of Artemisia, Lepidoptera, Dogwood, Dandelion, Red Pepper, Pine Needle, and Mixture thereof. The above-mentioned Inosugi, Lepidoptera, Cryptomeria japonica, Dandelion, Red pepper seeds or Pine needle can be used without any processing, but they can be used by cutting or in the form of an extract or powder. The process of processing into an extract or powder form and adding it to the cutting oil may be suitably carried out by a person skilled in the art.

In the present invention, the photosynthetic bacterial culture is prepared by inoculating cultured photosynthetic bacteria into a mixture of EM and nutrient medium (medium) mixed at a volume ratio of 1: 1 to 1: 5, specifically 1: 3. In this case, the fermentation temperature may be 10 ° C or higher, specifically 10 ° C to 30 ° C, more specifically 15 ° C to 25 ° C, and the fermentation period is 1 day or more, specifically 3 to 10 days, 5 to 8 days. The content of photosynthetic bacteria to be inoculated in the fermentation process may be from 0.1 to 20% by volume, specifically from 1 to 10% by volume, more specifically from 3 to 9% by volume, based on the total fermentation amount. According to an embodiment of the present invention, the content of the photosynthetic bacteria may be 3, 5, 8 or 10% by volume.

As used herein, the term "water soluble cutting oil" means machinability at the metal cutting, improvement in the degree of machining (surface roughness of the metal) and cooling, and oil used to extend tool life. The water-soluble cutting oil is generally used by being diluted with water. Water-soluble cutting oils include lubricating oils, lubricants, emulsifiers, surfactants, and water. In addition, other additives may include antifoaming agents, preservatives, coloring matters, perfumes, and the like. According to one embodiment of the present invention, the water-soluble cutting oil of the present invention may include a lubricating oil, an emulsifier, an extreme pressure additive and a defoaming agent.

The cutting oil may comprise one or more components selected from lubricating oils, emulsifiers, extreme pressure additives and defoamers. At this time, the types of the lubricating oil, the emulsifier, the extreme pressure additive and the defoaming agent can be appropriately selected by those skilled in the art, and the mixing ratio can be appropriately selected according to the required properties of the cutting oil.

The lubricant oil that can be included in the water-soluble cutting oil according to the present invention includes mineral oil, vegetable oil, sunflower oil, soybean oil, mustard oil, polyethylene glycol (PEG) ester, alcohol ester, PEG, poly (Ethylene oxide), P / O (propylene oxide), copolymer, _C10-13 chlorinated paraffin, sulfur containing ester, phosphorus extreme pressure additive, polyolefin (PAO) ≪ / RTI > and mixtures thereof. Examples of such sulfur containing esters may be selected from the group consisting of polysulfides, di-tert-dodecyl, KENLUBE SX 202, and mixtures thereof. An example of a phosphorus-containing extreme-pressure additive may be Reofos 65.

Meanwhile, emulsifiers that can be included in the water soluble cutting oil include PEG, amine, alcohol, tall oil fatty acid, oleic acid, isononanoic acid (INA), neodecanoic acid (NDA), octanoic acid (OTA) 9) octylphenol esters, polyoxyethylene lauryl esters, PEG esters, polyoxyethylene tridecyl esters, sorbitan monooleates, SM30 (polyoxyethylene (30) stearyl amines), acrylated fatty acid amides, polyethylene glycols and mixtures thereof Lt; / RTI >

Further, the extreme pressure additive may be selected from the group consisting of chlorinated paraffin, phosphorous ester, sulfur, and a mixture thereof. The antifoaming agent may be selected from the group consisting of 2-ethylhexanol, amorphous silica, and mixtures thereof. Amorphous silica is a family of denatured silicones, including AFE1226, AFE1266, LZ5674, and the like.

The water-soluble cutting oil according to the present invention may further contain an antirusting agent or an antiseptic agent if necessary.

The present invention relates to a method for culturing photosynthetic bacteria, Mixing the cultured photosynthetic bacteria with EM to secondary culture; And mixing the secondary culture liquid with a water-soluble cutting oil, wherein the rust-inhibiting effect is enhanced.

The photosynthetic bacteria, EM or culture liquid according to the present invention are as described above.

In the above production method, the photosynthetic bacteria according to the present invention may be contained in an amount of 0.1 to 20% by volume, 1 to 10% by volume and 3 to 9% by volume based on the total fermentation amount.

The fermentation can be carried out at a temperature of 10 ° C or higher, 10 ° C to 30 ° C, 15 ° C to 25 ° C, 1 day or more, 3 to 10 days or 5 to 8 days.

Hereinafter, the present invention will be described in detail with reference to examples. However, the following examples are intended to illustrate the present invention, but the present invention is not limited by the following examples.

I. Confirmation of rust inhibition effect of photosynthetic bacterial culture

Example 1 Production of Photosynthetic Bacterium Primary Culture Solution- (1)

Photosynthetic bacteria, Rhodobacter sphaeroides (Light Collection, Isaac, Korea), were cultured. Specifically, 500 ml of the photosynthetic bacteria were mixed with 20 g of ammonium chloride, 20 g of sodium hydrogencarbonate, 20 g of sodium acetate, 20 g of sodium chloride, 40 g of potassium hydrogen phosphate, 40 g of magnesium lactate, 2 g Of yeast extract was dissolved in water and cultured in a liquid medium with a total volume of 2 l. At this time, the incubation temperature was 20 ° C, and the photosynthetic bacteria to be inoculated was 4.0 x 10 ⁷ cfu / ml or more.

Among the growth curves of the microorganisms, microorganisms located at the attenuator were selected and used in the following experiments. After 4 days of cultivation, it was determined that the amount of gas generation was reduced and the dead body of the bacteria was observed and attained the attenuator, and the secondary culture was performed with the culture liquid after 4 days of culture.

Example 2: Preparation of photosynthetic bacterial primary culture - (2)

Rhodobacter spheroids (Doosan Eco Biznet, Korea), a photosynthetic bacterium, were cultured. The photosynthetic bacteria were cultivated in the culture medium provided with the culture medium (DS-PSB, Doosan Eco-Biznet, Korea). Specifically, 1 L of photosynthetic bacteria was cultured in a medium in which the total amount was adjusted to 100 L by dissolving the medium 1 (fertilizer component) in powder form and medium 2 (growth promoting component) in liquid form. At this time, the culture was carried out at 35 ° C, and the culture broth when the growth curve of the microorganism reached the attenuator was used for the secondary culture as described above.

Example 3: Preparation of secondary culture of photosynthetic bacteria - (1)

Four days after culturing in Example 1, Rhodobacter sphaeroids were inoculated into a group of useful microorganisms (EM, EM Bio, Korea) and molasses activity (EM Bio, Korea) and cultured at room temperature for 7 days. At this time, 0.1 kg of the photosynthetic bacteria of Example 1 was inoculated into a culture medium in which 0.4 kg of molasses and 0.3 kg of EM stock solution were dissolved in 19.2 kg of water, and cultured.

Example 4: Preparation of secondary culture of photosynthetic bacteria - (2)

The photosynthetic bacterial culture fluid on the fourth day of culturing in Example 2 was inoculated to various concentrations of EM (Comembaio, Korea) and molasses activity (Comembe Bio, Korea) at various concentrations and cultured at room temperature for 7 days. At this time, 0.1 kg of the photosynthetic bacteria of Example 2 was inoculated into a culture medium in which 0.4 kg of molasses and 0.3 kg of EM stock solution were dissolved in 19.2 kg of water and cultured.

Examples 5 to 8. Preparation of secondary cultures of photosynthetic bacteria - (3)

The culture of photosynthetic bacteria cultured in Example 2 was inoculated into a culture medium having the composition shown in the following Table 1 and cultured at room temperature for 7 days.

Example 2 Culture medium
(kg) molasses
(kg) EM solution
(kg) Salt
(kg) Sugar
(kg) water
(kg) Sum
(kg) Example 5 3.0 10 2.0 5 5 75.0 100 Example 6 5.0 10 2.0 5 5 73.0 100 Example 7 8.0 10 2.0 5 5 70.0 100 Example 8 10.0 10 2.0 5 5 68.0 100

Example 9. Preparation of secondary culture of photosynthetic bacteria added with Artemisia sp.

A secondary culture of photosynthetic bacteria supplemented with Artemisia mugwort was prepared in the same manner as in Example 8, except that 0.1 kg of Artemisia capillaris Thunb. Powder was further added to the culture medium.

Example 10: Preparation of secondary cultures of photosynthetic bacteria added with wild mugwort

A secondary culture of photosynthetic bacteria supplemented with Artemisia princeps Pampanini was prepared in the same manner as in Example 9 except that Artemisia princeps Pampanini was added instead of Artemisia mugwort.

Example 11 Preparation of Secondary Culture Medium of Photosynthetic Bacteria Containing Clam Mugwort

A secondary culture of photosynthetic bacteria added with Artemisia annua Linne was prepared in the same manner as in Example 9, except that Artemisia annua Linne was added instead of Artemisia mugwort.

Example 12: Preparation of secondary culture of photosynthetic bacteria added with dandelion

A secondary culture of photosynthetic bacteria added with dandelion was prepared in the same manner as in Example 9 except that taraxacum platycarpum was added instead of arginine .

Example 13. Preparation of secondary culture of photosynthetic bacteria added with red pepper seeds

A secondary culture of photosynthetic bacteria added with red pepper seeds was prepared in the same manner as in Example 9, except that seeds of Capsicum annuum were added instead of Artemisia sp.

Example 14: Preparation of photosynthetic bacteria culture solution with pine needle

A secondary culture of photosynthetic bacteria added with pine needle was prepared in the same manner as in Example 9, except that the leaves of Pinus densiflora were added instead of Artemisia sp .

Experimental Example 1. Confirmation of the rust prevention effect of the photosynthetic bacterial culture liquid

The photosynthetic bacterial culture solutions prepared in Examples 2 to 4 were each prepared to have an aqueous solution as shown in Table 2 below. Then, 30 g of iron pellets were added to the aqueous solution and the occurrence of rust was observed at room temperature.

Used photosynthesis
Bacterial culture solution Of photosynthetic bacterial culture
Dilution concentration (vol%) Whether rust is generated Time until rust occurs Control (water) 0.0 Occur 3 hours Example 2 0.3 Occur 4 hours Example 3 0.1 Occur 4 hours 0.3 Occur 5 hours 0.6 Occur 7 hours 1.0 Occur 8 hours 2.0 Occur After 5 days 3.0 Occur After 13 days Example 4 0.1 Occur 5 hours 0.3 Occur 6 hours 0.6 Occur 8 hours 1.0 Occur 9 hours Example 5 1.0 Occur 9 hours Example 6 1.0 Occur After 3 days Example 7 1.0 Occur After 4 days Example 8 1.0 Occur After 6 days

As shown in Table 2, the photosynthetic primary bacterial culture and the secondary culture of photosynthetic bacteria produced in Examples 2 to 8 of the present invention improved the anti-rust effect. This was proportional to the amount of the secondary culture of photosynthetic bacteria added.

In addition, the anticorrosive effects of the photosynthetic bacterial secondary culture solutions containing the antioxidant substances prepared in Examples 9 to 14 were confirmed in the same manner as described above. At this time, the culture was diluted to a concentration of 0.5 or 1.0% by volume.

Of photosynthetic bacterial culture
Dilution concentration (vol%) Whether rust is generated Time until rust occurs Control (water) 0.0 Occur 3 hours Example 9 0.5 Occur After 3 days 1.0 Occur After 7 days Example 10 0.5 Occur After 3 days 1.0 Occur After 7 days Example 11 0.5 Occur After 3 days 1.0 Occur After 7 days Example 12 0.5 Occur After 3 days 1.0 Occur After 7 days Example 13 0.5 Occur After 3 days 1.0 Occur After 7 days Example 14 0.5 Occur After 3 days 1.0 Occur After 7 days

As a result, as shown in Table 3, the rust preventing effect was enhanced in proportion to the dilution concentration of the photosynthetic bacterial culture solution, and the effect was further improved by the addition of the antioxidant.

Experimental Example 2. Confirmation of change of rust prevention effect after freezing and thawing

It was confirmed whether or not the secondary culture of the photosynthetic bacteria of the present invention retained the rust preventing effect even after freezing and thawing.

First, 10 kg of the photosynthetic bacterial primary culture prepared in Example 2 was mixed with 4.2 kg of molasses, 2.0 kg of EM stock solution, 0.3 kg of salt, 1.4 kg of sugar, 82 kg of water and 0.1 kg of arginine And then cultured at room temperature for 7 days to prepare a secondary culture of photosynthetic bacteria. The resulting secondary culture was frozen for 22 hours in a freezer (-15 ° C), and thawed at room temperature and stored at room temperature. The rust preventive effect was confirmed by preparing an aqueous solution containing 0.5 volume% of the secondary culture of photosynthetic bacteria, and then observing the occurrence of rust by adding 30 g of iron nail into the aqueous solution.

The results are shown in Table 4 below.

Whether to freeze Whether rust is generated Time until rust occurs Photosynthetic bacteria
Secondary culture ○ Occur After 3 days × Occur After 3 days

As shown in Table 4, the freezing of the culture solution did not affect the antirust effect.

II. Confirmation of rust inhibition effect of water-soluble cutting oil including photosynthetic bacterial secondary culture

Examples 15 and 16. Production of commercial water-soluble cutting oil to which photosynthetic bacterial culture solution was added - (1)

The secondary culture of photosynthetic bacteria prepared in Experimental Example 2 and the primary culture of photosynthetic bacteria prepared in Example 2 were diluted to a concentration of 2 vol% in the water-soluble cutting oil of Type 1 of the commercially available water-soluble cutting oil, 15 and 16, respectively, were prepared.

Examples 17 and 18. Preparation of commercial water-soluble cutting oil to which photosynthetic bacterial culture solution was added - (2)

The photosynthetic bacterial culture solutions of Examples 17 and 18 were contained in the same conditions and in the same manner as in Examples 15 and 16, except that the water-soluble cutting oil of No. 1 in No. 1 by Zeus Co. was used instead of the water-soluble cutting oil of No. 1 in No. 3 Water soluble cutting oil.

Comparative Examples 1 to 4 Production of commercial water-soluble cutting oil to which commercially available photosynthetic bacterial culture solution was added - (1)

(Japan), EM activated solution (Konem Biosar, Korea), and EM-K (Kobayashi Co., Ltd.) instead of the photosynthetic bacterial culture solution of Experimental Example 2, Water soluble cutting oil containing the photosynthetic bacterial culture solutions of Comparative Examples 1 to 4 was prepared by the same conditions and methods as in Example 15,

Comparative Examples 5 to 8. Preparation of commercial water-soluble cutting oil to which commercially available photosynthetic bacterial culture solution was added - (2)

The photosynthetic bacterial culture solutions of Comparative Examples 5 to 8 were prepared in the same manner as in Comparative Examples 1 to 4, except that the water-soluble cutting oil of Type 1 of No. 1 was used instead of the water-soluble cutting oil of No. 1, Water soluble cutting oil was prepared.

Experimental Example 3: Confirmation of enhancement of rust inhibition effect of water-soluble cutting oil by photosynthetic bacteria culture solution

The rust inhibitive effect of the water-soluble cutting oil prepared in Examples 15 to 18 and Comparative Examples 1 to 8 was confirmed. Specifically, the rust prevention effect was confirmed by observing occurrence of rust by adding 30 g of iron nail and 20 g of cast iron cast chip to the cutting oil. As a control, water - soluble cutting oil of the first grade or the first grade of Zeus `s No 1, which did not contain the photosynthetic bacterial culture solution, was used.

As a result, the results of confirming the rust inhibitive effect using the water-soluble cutting oil of Type 1 of No. 1, No. 1, and the results of confirming the rust inhibitive effect using the No. 1 No. 1 water-soluble cutting oil of Zeus Co. are shown in Table 6. In addition, photographs confirming the results are shown in Figs. 1 to 4. Fig.

Whether rust is generated Time until rust occurs 1 kind of 1 Occur After 1 day Example 15 Occur After 14 days Example 16 Occur 6 hours Comparative Example 1 Occur 4 hours Comparative Example 2 Occur 6 hours Comparative Example 3 Occur After 1 day Comparative Example 4 Occur After 1 day

Whether rust is generated Time until rust occurs Zeus 1 kind 1 Occur After 1 day Example 17 Occur After 15 days Example 18 Occur 2 hours Comparative Example 5 Occur 2 hours Comparative Example 6 Occur 1 hours Comparative Example 7 Occur 27 hours Comparative Example 8 Occur 20 hours

As a result, as shown in Tables 5 and 6, when the photosynthetic bacterial culture solution of the present invention was added, the rust inhibitive effect of commercially available water-soluble cutting oil was enhanced (Examples 15 and 17). However, in Comparative Examples 1, 2, 5 and 6 and Examples 16 and 18, the time to rust generation was shorter than that of the water-soluble cutting oil of No. 1 or No. 1, No. 1, in which no additives were added. This is because the added photosynthetic bacteria break down the emulsion form of the water-soluble cutting oil and thus the anti-rust effect is deteriorated.

III. Confirmation of anti-rust effect of water-soluble cutting oil including photosynthetic bacterial culture liquid without rust inhibitor and preservative

Example 19 Production of water-soluble cutting oil containing photosynthetic bacteria secondary culture solution- (1)

A water-soluble cutting oil free of rust preventives and preservatives was prepared using the photosynthetic bacterial secondary culture prepared in Example 3.

Specifically, 2% by volume of photosynthetic bacteria secondary culture, 40% by volume of mineral oil base oil (Zeus oil base, Korea), 10% by volume of seed oil (Chung Kyung Industry Co., Korea), 5% by volume of chlorinated paraffin 5% by volume of tall oil fatty acid (TOFA) (GNO, Korea), 5% by volume of stearyl amine 30 (emulsifier) 15 vol.% Boric amide (Cosmos FNC, Korea), 5 vol.% Dibasic acid (polyoxyethylene (9) octylphenol ether, SJ Chem, Korea) (Cosmos FNC, Korea), 2 vol% DCHA (dicyclohexylamine) (SPC, Korea), 0.2 vol% 2-ethylhexanol defoamer (Sungwon PLS, Korea) and 6.8 vol% Water soluble cutting oil was prepared.

Example 20: Preparation of water-soluble cutting oil containing photosynthetic bacteria secondary culture solution - (2)

Instead of the secondary culture of photosynthetic bacteria prepared in Example 3 A water-soluble cutting oil was prepared under the same conditions and in the same manner as in Example 19, except that the photosynthetic bacterial secondary culture prepared in Example 4 was used.

Example 21: Preparation of water-soluble cutting oil containing photosynthetic bacteria secondary culture solution - (3)

A water-soluble cutting oil was prepared under the same conditions and in the same manner as in Example 19, except that the photosynthetic bacterial secondary culture prepared in Example 9 was used instead of the photosynthetic bacterial secondary culture prepared in Example 3.

Comparative Example 9. Preparation of water-soluble cutting oil containing no photosynthetic bacteria

A water-soluble cutting oil was prepared in the same manner as in Example 19, except that the photosynthetic bacterial secondary culture was not added.

Comparative Example 10. Preparation of water-soluble cutting oil containing primary culture of photosynthetic bacteria

A water-soluble cutting oil was prepared under the same conditions and in the same manner as in Example 19, except that the photosynthetic bacterial primary culture prepared in Example 2 was used instead of the photosynthetic bacterial secondary culture prepared in Example 3.

Comparative Example 11. Preparation of water-soluble cutting oil containing useful microorganism group- (1)

The photosynthetic bacterial secondary culture prepared in Example 3 A water-soluble cutting oil was prepared in the same manner as in Example 19, except that the useful microorganism group of EM Bioscience was added.

Comparative Example 12. Preparation of water-soluble cutting oil containing useful microorganism groups

The photosynthetic bacterial secondary culture prepared in Example 3 A water-soluble cutting oil was prepared in the same manner as in Example 19, except that the useful microorganism group of Comem Biotech was added instead.

Experimental Example 4. Confirmation of rust-inhibitive effect of water-soluble cutting oil including photosynthetic bacteria secondary culture

Aqueous coolant solution prepared by diluting the water-soluble cutting oil prepared in Examples 19 to 21 and Comparative Examples 9 to 12 to 2.0 vol% was added with 30 g of nails and 20 g of metal chips into the aqueous solution, Were observed.

The results are shown in Table 7 below.

Whether rust is generated Time until rust occurs Example 19 Occur After 3 days Example 20 Occur After 4 days Example 21 Occur After 6 days Comparative Example 9 Occur 5 hours Comparative Example 10 Occur 5 hours Comparative Example 11 Occur 6 hours Comparative Example 12 Occur 5 hours

As a result, as shown in Table 7, the water soluble cutting oil of Examples 19 to 21 containing the photosynthetic bacterial secondary culture of the present invention rusted after 3 to 6 days, while the water soluble cutting oil of Comparative Examples 9 to 12 , Rust occurred after 5 to 6 hours. From these results, it was found that the photosynthetic bacterial secondary culture of the present invention exhibits excellent antirust effect.

IV. Identification of antibacterial activity of water-soluble cutting oil

The antibacterial activity of the water-soluble cutting oil containing the photosynthetic bacterial culture solution of the present invention was confirmed through the following experiments.

First, a preservative water-soluble cutting oil A-1 (vegetable base oil type), a water-insoluble water-soluble cutting oil A-2 (synthetic base oil type) and a preservative water-soluble cutting oil A-3 (preservatives such as BIT, MIT, 2 (synthetic base oil type), preservative water-soluble cutting oil B-3 (same composition as B-2, BIT, MIT, CMIT and the like) was prepared as follows.

A-1: 2% by volume of the photosynthetic bacterial culture solution of Example 9, 40% by volume of soybean oil (Cheon Kyung Industry Co., Korea), 10% by volume of seed oil (Cheon Kyung Industry Co., 10% by volume of boric acid (Cosmo FNC, Korea), 11% by volume of dibasic acid (GNO, Korea), 5 vol% SM30 (COSMOS FNC, Korea), 2 vol% DCHA (SPC, Korea), 0.2 vol% 2-ethylhexanol (Sungwon PLS, Korea) and 6.8 vol% water were mixed to prepare a water-soluble cutting oil.

A-2: A water-soluble cutting oil having the same composition as A-1 was prepared, except that synthetic base oil (Zeus oil, Korea) was used instead of soybean oil.

A-3: MBX (Daicheng Co., 5-chloro-2-methyl-4-isothiazolin-3-one (1.1 wt%) and 1, (Containing 4% by weight of 2-benzisothiazolin-3-one) were mixed to prepare a water-soluble cutting oil.

B-1: 2% by volume of the photosynthetic bacterial culture solution of Example 9, 40% by volume of soybean oil (Cheon Kyung Industry Co., Korea), 10% by volume of seed oil (Cheon Kyung Industry Co., (Cosmos FNC, Korea), 7 vol% dibasic acid (Cosmos FNC, Korea), 7 vol% of SM30 (Dongnam Synthetic Co., Korea), 7 vol% of monopole OP1019 (SPC, Korea), 2 volume% DCHA (SPC, Korea), 0.2 volume% 2-ethylhexanol (Sungwon PLS, Korea) and 6.8 vol% water were mixed to prepare a water-soluble cutting oil.

B-2: A water-soluble cutting oil having the same composition as B-1 was prepared, except that a synthetic base oil (Zeus oil, Korea) was used instead of soybean oil.

B-3: MBX (Daicheng Co., 5-chloro-2-methyl-4-isothiazolin-3-one (1.1 wt%) and 1, (Containing 4% by weight of 2-benzisothiazolin-3-one) were mixed to prepare a water-soluble cutting oil.

Experimental Example 5: Microbial contamination analysis

30 ml of the cutting oil of the above A-1 to B-3 was inoculated into a tryptic soy agar medium (TSA medium) and then cultured at 30 ° C for 3 days.

As a result of observing the growth of microorganisms in each sample, microorganisms such as bacteria and fungi were not detected in any sample. Therefore, it can be seen that not all samples are contaminated by microorganisms.

Experimental Example 6: Microbial kill effect analysis (Challenge test)

To confirm the antimicrobial activity of the cutting oil to which the photosynthetic microorganism was added, the test bacteria mixture was inoculated to the cutting oils of A-1 to B-3 in an amount of 10 ⁶ cfu / ml.

The test mixture was prepared from Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 15442, Burkholderia cepacia ATCC 15442, Klebsiella pneumoniae ATCC 4352, But are not limited to, Enterobacter aerogenes ATCC 13048, Salmonella typhimurium KCTC 1925, Staphylococcus aureus ATCC 6538, Mycrococcus luteus ATCC 9341, Candida albicans , , And Agaricus brasiliensis .

Immediately after strain inoculation, the obtained test solution was plated on TSA medium and cultured at 30 ° C for 3 days. In addition, the test solution was stained on TSA medium 2 days after storage at 30 DEG C, and cultured in the same manner as above, and then the growth of the strain was observed.

The results of the growth of the strain are shown in Table 8 below.

Coolant sample Early Two days later A-1 3 0 A-2 3 0 A-3 3 0 B-1 3 0 B-2 3 0 B-3 3 0

Rating system

0: No bacteria

1: trace contamination (1-9 colonies) 3: medium contamination (> 100 colonies)

2: Weak pollution (10-99 colonies) 4: Severe contamination (TNTC)

As can be seen from the results of Table 8, 100 colonies or more were produced at the initial stage of the inoculation of the strain, but both of the cutting oil to which the photosynthetic bacterial culture fluid was added and the preservative-added cutting oil showed antimicrobial activity 2 days after the culturing.

Claims (17)

As a water-soluble cutting oil containing a photosynthetic bacterial culture liquid,
The cutting oil is improved in rust prevention effect,
Wherein the photosynthetic bacterial culture solution is a secondary culture obtained by mixing and culturing at least one microorganism selected from lactic acid bacteria, yeast and Bacillus subtilis with a photosynthetic bacterium and culturing the obtained primary culture with a useful microorganism group (EM)
Wherein the photosynthetic bacteria are Rhodobacter sphaeroides ,
Wherein the secondary culture solution is obtained by culturing a primary culture in a culture medium containing any one selected from the group consisting of Artemisia, Lepidoptera, Dogwood, Dandelion, Red Pepper, Pine Needle and Mixture thereof,
Wherein the photosynthetic bacterial culture fluid is contained in an amount of 0.1 to 5% by volume based on the total volume of water-soluble cutting oil.
delete delete delete delete The water-soluble cutting oil according to claim 1, wherein the culture liquid is obtained by culturing a photosynthetic bacteria in a medium selected from the group consisting of molasses, wheat bran, rice bran, and a mixture thereof.
delete delete delete The water-soluble cutting oil according to claim 1, wherein the cutting oil is composed of a lubricating oil, an emulsifier, an extreme pressure additive and an antifoaming agent.
The method of claim 10, wherein the lubricating oil is selected from the group consisting of mineral oil, vegetable oil, sunflower oil, soybean oil, mustard oil, polyethylene glycol (PEG) ester, alcohol ester, PEG, polyalkylene glycol ethylene oxide, P / O, copolymer, C _10-13 chlorinated paraffin, sulfur containing ester, poly alpha olefin (PAO), and mixtures thereof. And water-soluble cutting oil.
11. The method of claim 10, wherein the emulsifier is selected from the group consisting of PEG, amine, alcohol, tall oil fatty acid, oleic acid, isononanoic acid, neodecanoic acid, octanoic acid, Octylphenol ester, polyoxyethylene lauryl ester, PEG ester, polyoxyethylene tridecyl ester, sorbitan monooleates, SM30 (stearyl amine 30), acrylated fatty acid amide, polyethylene glycol, and mixtures thereof And water-soluble cutting oil.
11. The water soluble cutting oil according to claim 10, wherein said extreme pressure additive is selected from the group consisting of chlorinated paraffin, phosphorous ester, sulfur and mixtures thereof.
The water-soluble cutting oil according to claim 10, wherein the defoaming agent is selected from the group consisting of 2-ethylhexanol, amorphous silica and mixtures thereof.
Primary culturing photosynthetic bacteria;
Mixing the cultured photosynthetic bacteria with a useful microorganism group (EM) to secondary culture; And
Mixing the secondary culture with water-soluble cutting oil,
Wherein the primary culture comprises mixing at least one microorganism selected from lactic acid bacteria, yeast and Bacillus subtilis with photosynthetic bacteria,
Wherein the photosynthetic bacteria are Rhodobacter sphaeroides ,
Wherein the secondary culture is cultivated in a culture medium containing any one selected from the group consisting of Artemisia, Lepidoptera, Dogwood, Dandelion, Red Pepper, Pine Needle, and Mixture thereof,
Wherein said mixing is carried out in an amount of 0.1 to 5% by volume based on the total volume of water-soluble cutting oil.
delete The method according to claim 15, characterized in that the secondary culture is carried out at a temperature of 15 캜 to 25 캜.

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