KR101741104B1 - An High-capacity Field Collecting and Concentrating System for Waterborne Pathogen - Google Patents

An High-capacity Field Collecting and Concentrating System for Waterborne Pathogen Download PDF

Info

Publication number
KR101741104B1
KR101741104B1 KR1020150123685A KR20150123685A KR101741104B1 KR 101741104 B1 KR101741104 B1 KR 101741104B1 KR 1020150123685 A KR1020150123685 A KR 1020150123685A KR 20150123685 A KR20150123685 A KR 20150123685A KR 101741104 B1 KR101741104 B1 KR 101741104B1
Authority
KR
South Korea
Prior art keywords
enrichment
pathogen
water
environmental sample
concentration
Prior art date
Application number
KR1020150123685A
Other languages
Korean (ko)
Other versions
KR20170027137A (en
Inventor
곽노균
강지윤
이택진
Original Assignee
한국과학기술연구원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 한국과학기술연구원 filed Critical 한국과학기술연구원
Priority to KR1020150123685A priority Critical patent/KR101741104B1/en
Publication of KR20170027137A publication Critical patent/KR20170027137A/en
Application granted granted Critical
Publication of KR101741104B1 publication Critical patent/KR101741104B1/en

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4005—Concentrating samples by transferring a selected component through a membrane
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N2001/4038—Concentrating samples electric methods, e.g. electromigration, electrophoresis, ionisation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The present invention relates to a system capable of collecting and concentrating only water-borne pathogens by collecting environmental samples from rivers or lakes, and more particularly to a system capable of collecting and concentrating waterborne pathogens from underwater environmental samples at a high collection rate and concentration ratio. And more particularly to a large capacity waterborne pathogen capture and enrichment system capable of regulating the collection and concentration ratio as needed utilizing a modular waterborne pathogen enrichment module.

Description

Field of the Invention < RTI ID = 0.0 > [0002] < / RTI > An High Capacity Field Collecting and Concentrating System for Waterborne Pathogen &

The present invention relates to a system capable of collecting and concentrating only water-borne pathogens by collecting environmental samples from rivers or lakes, and more particularly to a system capable of collecting and concentrating waterborne pathogens from underwater environmental samples at a high collection rate and concentration ratio. And more particularly to a large capacity waterborne pathogen capture and enrichment system capable of regulating the collection and concentration ratio as needed utilizing a modular waterborne pathogen enrichment module.

A system for collecting water-borne pathogens present in large-volume environmental samples such as rivers, lakes and reservoirs has been steadily increasing in demand for environmental monitoring. The system for collecting water-borne pathogens is a system that passes environmental samples (liquids) through a micro-filter, induces pathogens present in the sample to adhere to the filter, separates the filter, The adsorption-elution method proposed by the US EPA as a standard method, the sample is diluted with a stock solution or a buffer solution in a tube as shown in the following literature, and then the nano- (Ultracentrifugation) in which pathogens are obtained.

<Articles>

Lee Seung-Hoon, Kim Sang-Jong, "Detection of infectious enteroviruses and adenoviruses in tap water in urban areas in Korea", Water Research, Vol. 36, No.1, 248 to 256p, 0043 to 1354

However, the above-mentioned adsorption-elution method has a problem in that the collection rate of specific pathogens such as avian influenza is remarkably decreased (<10%). This low collection rate is critical to the monitoring of infectious pathogens (such as avian influenza) that are important for confirmation and needs to be improved. In addition, the ultracentrifugation method requires expensive equipments and skilled techniques, and centrifugal force has a problem that the smaller the size of the target particle, the sudden decrease of the centrifugal force, and the sufficient pathogen can not be collected.

SUMMARY OF THE INVENTION The present invention has been made to solve the above problems,

It is an object of the present invention to provide a large-capacity waterborne pathogen in situ capture and concentration system that enables rapid capture and concentration of waterborne pathogens from environmental samples in water at high collection rates and concentration ratios.

Another object of the present invention is to provide a large-capacity water-borne pathogen capture and enrichment system capable of regulating the collection, collection rate and concentration ratio as needed by utilizing a modular water-borne pathogen enrichment module.

It is a further object of the present invention to provide a large-capacity waterborne pathogen in situ collection and concentration system that can alter the electric field and / or filter membrane of the concentrate and, if necessary, control the subject or the collection or removal and concentration ratio of the waterborne pathogen will be.

In order to achieve the above-mentioned object of the present invention, a large capacity waterborne pathogen in situ collection and concentration system includes the following constitution.

The large-capacity water-borne pathogen on-site collection and concentration system according to an embodiment of the present invention includes a plurality of modular water-borne pathogen enrichment modules, and the environmental samples collected in the field sequentially mount a plurality of water- And the water-borne pathogen is concentrated at a high concentration.

In another aspect of the present invention, there is provided a system for collecting and concentrating a large-capacity water-borne pathogenic organism, which comprises an enrichment unit for fractionating water-borne pathogens in an environmental sample flowing at a constant rate using an electric field and a micro- .

In one embodiment, the at least one enrichment module of the plurality of enrichment modules may include at least one enrichment module that energizes the enrichment module to flow through the enrichment module at a constant rate, And a fluid power supply unit.

In the large scale water-borne pathogen on-site collection and concentration system according to another embodiment of the present invention, among the plurality of concentration modules, the concentration module through which the environmental sample first passes includes a pretreatment filter .

In the system for collecting and concentrating large-scale water-borne pathogenic organisms according to another embodiment of the present invention, the enrichment unit may include an electrode for generating an electric field at upper and lower portions of a flow portion through which the introduced environmental sample flows, A filter membrane for separating the space of the flow portion at a predetermined height and a buffer flow portion positioned at the upper and lower sides of the flow portion and allowing the electric field to be transmitted to the flow portion.

In accordance with another embodiment of the present invention, there is provided a system for collecting and concentrating large-capacity water-borne pathogenic organisms, the filter membrane comprising: a fluid of an environmental sample flowing into the fluidizing unit in the fluidizing unit; And separating the fluid containing the fluid.

The present invention can obtain the following effects by the above-described embodiment, the constitution described below, the combination, and the use relationship.

INDUSTRIAL APPLICABILITY The present invention has an effect of rapidly collecting and concentrating a water-borne pathogenic organism from an environmental sample in water at a high collection ratio and a concentration ratio.

The present invention utilizes a modular waterborne pathogen enrichment module to control the collection, feeding speed and concentration ratio as needed.

The present invention has the effect of modifying the electric field and / or the filter membrane of the concentrated portion so that the object of the water-borne pathogen or the collection or removal of the water-borne pathogen and the concentration ratio can be adjusted.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic block diagram of a large volume waterborne pathogen in situ collection and concentration system in accordance with an embodiment of the present invention. FIG.
FIG. 2 is a schematic view showing a detailed configuration and an operation process of the enrichment unit of FIG. 1; FIG.
FIG. 3 is a fluorescence microscope photograph showing an operating state of the enrichment unit according to an embodiment of the present invention. FIG.
4 is a graph showing the operation result of the enrichment unit according to an embodiment of the present invention.

Hereinafter, a large capacity waterborne pathogen on-site collection and concentration system according to the present invention will be described in detail with reference to the accompanying drawings. Unless defined otherwise, all terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs and, if conflict with the meaning of the terms used herein, It follows the definition used in the specification. Further, the detailed description of known functions and configurations that may unnecessarily obscure the subject matter of the present invention will be omitted. Throughout the specification, when an element is referred to as "including " an element, it is understood that the element may include other elements as well, without departing from the other elements unless specifically stated otherwise.

The large-capacity water-borne pathogen on-site collection and concentration system according to an embodiment of the present invention will now be described with reference to FIGS. 1 to 4. The large-capacity waterborne pathogen on-site collection and concentration system includes a concentration module 1), and an environmental sample collected in the field is sequentially passed through the plurality of concentration modules (1) so that the water-borne pathogen is concentrated to a high concentration. The concentration module 1 is connected in series and / or in parallel. When the concentration module 1 is connected in series as shown in FIG. 1, the environmental sample passes through each concentration module 1, Is increased. For example, assuming that each concentration module 1 is capable of 20-fold concentration of the water-borne pathogen relative to the environmental sample and that the three concentration modules 1 are connected in series, the water- Is concentrated 20 3 times as much as the input environmental sample. Although not shown, when the concentrating modules 1 are connected in parallel, the collection rate (total throughput) of the water-borne pathogen can be increased. Accordingly, the various numbers of concentration modules 1 can be arranged in series and / or in parallel so that the required concentration ratio and throughput can be easily obtained. Since the environment sample collected at the site of the river, river, reservoir, etc. flowing into the collection and concentration system contains many foreign substances, a mesh net (2 mesh) for removing foreign substances is disposed at the front end of the first concentration module (1a) May be further included.

The enrichment module 1 includes a fluid power supply unit 11 for supplying power such that the environmental sample can flow through the concentrating unit 12 at a constant speed and flows by the power provided by the fluid power supply unit 11. [ And an enrichment unit 12 for concentrating the water-borne pathogen in the environmental sample. One fluidizing power supply unit 11 and one enrichment unit 12 are modularized to form one enrichment module 1. If necessary, the fluidity power supply unit 11 may be connected to only the specific enrichment module 1 It is also possible to include it. Various means capable of providing a power for moving the environmental sample can be used as the hydraulic power supply unit 11, for example, a hydraulic pump or the like. In addition, the concentration module 1a through which the environmental sample passes first among the plurality of concentration modules 1 may further include a pretreatment filter 13 that removes suspended substances having a predetermined size or larger in advance. That is, as in the example shown in Fig. 1, a separate pretreatment filter 13 (prefilter, preferably a water filter) is provided before the environmental sample introduced into the system is first injected into the thickening section 12a of the concentration module 1a. um) filter, so that unnecessary floating matters or foreign substances are removed in advance before entering the concentration unit 12a, thereby enhancing the efficiency of fractionation concentration action of the water-borne pathogen in the concentration unit 12a.

The enrichment unit 12 is configured to concentrate the water-borne pathogen in an environmental sample moving by the power supplied by the flow-power supply unit 11, and is configured to concentrate the water-borne pathogen at a constant rate using an electric field and a micro- Water-borne pathogens are fractionally concentrated in flowing environmental samples.

The concentrated portion 12 includes an oil flowing portion 121 through which the introduced environmental sample flows, an electrode 122 positioned above and below the oil flowing portion 121 to generate an electric field, A filter membrane 123 for separating the space of the fluid section 121 at a predetermined height along the longitudinal direction, a cation exchange membrane 124 located on the upper and lower sides of the fluid section 121, And a buffer flow part 125 formed between the electrode 122 and the buffer 122 to move the buffer solution.

The flow unit 121 is configured such that the introduced environmental sample is moved. The flow unit 121 is partitioned at a predetermined height by the filter membrane 123, and the environmental sample flows into the lower part, And a pathological channel 121b in which the water channel 121a is discharged after the water channel 121 is removed and the water channel introduced into the waste channel 121a through the filter membrane 123 is concentrated and discharged do. As shown in FIG. 3 (a), the fluids of the waste channel 12a and the pathogen channel 121b are caused to flow in the same direction (Parallel-flow operation) The fluid in the waste channel 12a and the fluid in the pathogen channel 121b may flow in different directions as shown in FIG.

The electrodes 122 are located on upper and lower sides of the fluid portion 121 so that an electric field is generated in the concentrated portion 12.

The filter membrane 123 is configured to separate the space of the fluid portion 121 at a predetermined height along the longitudinal direction of the fluid portion 121 and to allow passage of water pathogens such as viruses and bacteria lt; RTI ID = 0.0 &gt; um. &lt; / RTI &gt; A substance larger than several um in the environmental sample that has not been removed by the mesh net 2 and the pretreatment filter 13 can not pass through the filter membrane 123 and can be introduced into the waste channel 121a of the fluid section 121 It remains.

The cation exchange membrane 124 is positioned on the upper and lower sides of the flow portion 121 to allow ions to move by the electrode 122.

The buffer flow unit 125 is formed between the cation exchange membrane 124 and the electrode 122 to move the buffer solution. As the buffer solution, for example, NaCl, KCl, or the like may be used. Buffer flow units 125 are positioned on upper and lower sides of the flow unit 121 through which the environmental sample flows so that an electric field is transmitted to the concentration unit 12, So that the reaction product due to the electrochemical reaction occurring in the reaction chamber is not influenced by the concentration.

A method for concentrating water-borne pathogens such as viruses and bacteria using the thickening part 12 having the above-described structure will be described. In the concentrated part 12 constructed as shown in FIG. 2, through the inflow part of the waste channel 121a When an environmental sample is introduced and power is supplied through the electrode 122, an ion concentration polarization occurs in the lower cation exchange membrane 124a, and all charged substances such as water-borne pathogens are directed upward The water permeable pathological substance smaller in size than the pores of the filter membrane 123 located at a certain height of the fluid 121 passes through the filter membrane 123 and collects in the pathological channel 121b, Only foreign matter or other particles remain in the discharge portion of the waste channel 121a, and it becomes possible to concentrate the water-borne pathogen in the environmental sample. The concentration unit 12 adjusts the concentration ratio of the target water-borne pathogen by adjusting the voltage applied to the electrode 122, the pore size of the filter membrane 123, the flow rate of the waste channel 121a, Can be adjusted. 2, the other conditions are the same, the pore size of the filter membrane 123 is 1 mu m, the flow rate of the pathogen channel 121b is 20 mu L / min, 121a) at a flow rate of 280 uL / min, and an environmental sample containing a water-borne pathogen (fluorescence particle) having a fluorescence dye dyed less than 1 um was supplied and supplied with a constant voltage, It was possible to concentrate human pathogens. 3 (a) is a photograph of a part of the concentrated portion 12 taken under a fluorescence microscope under the above conditions. It can be seen that the pathogen channel 121b shines brightly on the waste channel 121a, It can be seen that the fluorescent particles are concentrated in the pathogen channel 121b. 3 (b) is a result of experiment under different conditions from FIG. 3 (a). It can also be seen that the pathogen channel 121b of FIG. 3 (b) also shines brightly on the waste channel 121a , It can be seen that the fluorescent particle is concentrated in the pathogen channel 121b. 4 is a graph showing the fluorescent intensity measured according to the distance A-A 'of the thickening part 12 as shown in FIG. 3 (a) It can be confirmed that fluorescence particles can be enriched up to 20 times when a voltage is supplied for 150 seconds in comparison with the case (0 sec).

While the present invention has been described in connection with what is presently considered to be practical exemplary embodiments, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, Should be interpreted as belonging to the scope.

1: enrichment module 2: mesh network 11: fluid power supply
12: enrichment unit 13: pretreatment filter 121:
122: electrode 123: filter membrane 124: cation exchange membrane
125: buffer flow unit 121a: waste channel 121b: pathogen channel

Claims (6)

Comprising a plurality of modular waterborne pathogen enrichment modules,
The concentration module includes an enrichment unit for concentrating the water-borne pathogen in an environmental sample flowing at a constant rate using an electric field and a micro-unit filter membrane,
Wherein the enrichment unit comprises an electrode for generating an electric field at upper and lower portions of a flow portion through which the introduced environmental sample flows, a filter membrane for separating the space of the flow portion at a predetermined height along the longitudinal direction of the flow portion, And a buffer flow portion for allowing an electric field to be transmitted to the flow portion. delete The method according to claim 1,
Wherein the at least one enrichment module of the plurality of enrichment modules further comprises a fluid power supply that provides power to allow the environmental sample to flow through the enrichment module at a constant rate. . The method according to claim 1,
Wherein the enrichment module through which the environmental sample first passes among the plurality of enrichment modules further includes a pretreatment filter for previously removing suspended matters having a predetermined size or larger. delete The method according to claim 1,
Wherein the filter membrane separates the fluid of the environmental sample flowing into the flow section in the flow section and the fluid containing the water-based pathogen separated through the filter membrane.
KR1020150123685A 2015-09-01 2015-09-01 An High-capacity Field Collecting and Concentrating System for Waterborne Pathogen KR101741104B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020150123685A KR101741104B1 (en) 2015-09-01 2015-09-01 An High-capacity Field Collecting and Concentrating System for Waterborne Pathogen

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020150123685A KR101741104B1 (en) 2015-09-01 2015-09-01 An High-capacity Field Collecting and Concentrating System for Waterborne Pathogen

Publications (2)

Publication Number Publication Date
KR20170027137A KR20170027137A (en) 2017-03-09
KR101741104B1 true KR101741104B1 (en) 2017-05-30

Family

ID=58402394

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020150123685A KR101741104B1 (en) 2015-09-01 2015-09-01 An High-capacity Field Collecting and Concentrating System for Waterborne Pathogen

Country Status (1)

Country Link
KR (1) KR101741104B1 (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010223728A (en) 2009-03-23 2010-10-07 National Institute For Agro-Environmental Science Sampler, sampling method, and sampling system
JP2011500025A (en) 2007-10-09 2011-01-06 ユニヴァーシティー オブ ノートル ダム デュ ラック Microfluidic platform for detecting multiple types of targets
KR101157176B1 (en) 2005-12-20 2012-06-20 삼성전자주식회사 Microfluidic device and method for concentration or purification of cells or viruses
KR101157175B1 (en) 2005-12-14 2012-07-03 삼성전자주식회사 Microfluidic device and method for concentration and lysis of cells or viruses
KR101222695B1 (en) 2012-08-28 2013-01-16 (주)대진환경개발 Apparatus for treating sewage using electric field pretreatment and membrane

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101157175B1 (en) 2005-12-14 2012-07-03 삼성전자주식회사 Microfluidic device and method for concentration and lysis of cells or viruses
KR101157176B1 (en) 2005-12-20 2012-06-20 삼성전자주식회사 Microfluidic device and method for concentration or purification of cells or viruses
JP2011500025A (en) 2007-10-09 2011-01-06 ユニヴァーシティー オブ ノートル ダム デュ ラック Microfluidic platform for detecting multiple types of targets
JP2010223728A (en) 2009-03-23 2010-10-07 National Institute For Agro-Environmental Science Sampler, sampling method, and sampling system
KR101222695B1 (en) 2012-08-28 2013-01-16 (주)대진환경개발 Apparatus for treating sewage using electric field pretreatment and membrane

Also Published As

Publication number Publication date
KR20170027137A (en) 2017-03-09

Similar Documents

Publication Publication Date Title
JP6458047B2 (en) Particulate separation device and related method and system
US8999132B2 (en) Desalination and purification system
JP5323933B2 (en) Pump supply switching and desalting of amplified dynamic current bodies
US10252924B2 (en) Purification of ultra-high saline and contaminated water by multi-stage ion concentration polarization (ICP) desalination
CN102225812B (en) Membrane treatment process for oilfield reinjection water
RU2630541C2 (en) Saline water treatment device and method
US7811439B1 (en) Concentration and separation of biological organisms by ultrafiltration and dielectrophoresis
DE60230449D1 (en) DEVICE AND METHOD FOR SEPARATING AND DEFLECTING PARTICLES AND ORGANISMS FROM FLOWING LIQUIDS
CN203781971U (en) Gas field produced water desalting treatment equipment
CN101219836A (en) System and technique for processing oily wastewater with high saline salinity
CN103130363B (en) Desalination system and desalination method
CN103922499B (en) A kind of heavy metal ions in wastewater eddy flow strengthening extraction method and device thereof
US9956529B2 (en) Microfabricated ion-selective filter for filtration of ions and molecules
Lee et al. Electrokinetic-assisted filtration for fast and highly efficient removal of microplastics from water
CN200995983Y (en) Efficient automatic water purifier
KR101741104B1 (en) An High-capacity Field Collecting and Concentrating System for Waterborne Pathogen
WO2019147802A9 (en) Return flow system for ion concentration polarization (icp) desalination
CN210480932U (en) Separation and concentration equipment for high-concentration high-chroma high-salinity degradation-resistant organic wastewater
CN108383254A (en) A kind of medium vibration-type continuous separation method and device
US20180319681A1 (en) Contactless Ion Concentration Method &amp; Apparatus Using Nanoporous Membrane with Applied Potential
KR101727979B1 (en) An unmanned environmental sample collection system
Mohammadimehr et al. Impact of microfluidic cell and particle separation techniques on microplastic removal strategies
Robertson et al. Treatment of high TDS liquid waste: Is zero liquid discharge feasible?
KR102418388B1 (en) Water purifier for agriculture
JP2008221058A (en) Separation concentration apparatus of water microorganisms

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
E701 Decision to grant or registration of patent right
GRNT Written decision to grant