KR101726210B1

KR101726210B1 - Operating method for Extracting device of regenerative cells

Info

Publication number: KR101726210B1
Application number: KR1020150124222A
Authority: KR
Inventors: 김태영
Original assignee: 주식회사 베스테크
Priority date: 2015-09-02
Filing date: 2015-09-02
Publication date: 2017-04-26
Also published as: KR20170027504A

Abstract

The present invention relates to a method of operating an apparatus for extracting regenerative cells from adipose tissue, and more particularly, to an apparatus and a method for extracting regenerative cells from adipose tissue, Not only can it proceed easily, but also shorten the extraction time of cells and efficiently collect and obtain regenerated cells.
The present invention relates to a method of operating an apparatus for separating and extracting regenerative cells from adipose tissue,
An input setting step of inputting an amount of fat to be input and processed into a regenerative cell extractor by a user using a touch screen of the main body and selecting and inputting an automatic and manual progress mode;
An adipose tissue washing step of introducing adipose tissue into a mixing chamber, injecting injection water such as saline, and rotating the unit of the mixing chamber to mix and wash the adipose tissue and the injection water;
An enzyme mixing step of injecting an enzyme into the washing fat in the mixing chamber and rotating the unit into which the washing fat and the enzyme have been introduced so as to mix the fat and the enzyme;
Centrifuging the mixture in a mixing chamber using a peristaltic pump and a transfer tube to a rotary container of a centrifugal separator, rotating the rotary container, and separating and concentrating the cells by centrifugation;
The peritoneal pump is operated to transfer the water to the rotary chamber of the centrifugal separator through the transfer tube and to inject the water into the water chamber and rotate the rotary vessel to wash and concentrate the cells, A cell washing step in which the cell washing step is performed;
A cell washing confirmation step of confirming whether or not the number of times of washing of cells set as an input to the control unit has been reached;
A cell recovery step of separating and extracting washed cells in a rotating container of a centrifugal separator to recover cells through a transfer tube;
. &Lt; / RTI >

Description

[0001] The present invention relates to an operating method for extracting regenerative cells,

The present invention relates to a method of operating an apparatus for extracting regenerative cells from adipose tissue, and more particularly, it relates to a method of automatically extracting regenerated cells, In addition, it is possible to shorten the extraction time of cells and to efficiently collect and acquire regenerative cells.

Generally, the fat cells (PLA: Processed Lipo Aspirate) obtained through the fat extraction process include fat cells and many stem cells, and thus utilize surplus fat.

In the past, adipose tissue was regarded only as a caloric storage structure that stores surplus energy, and it was regarded as an object to be removed only. Recently, as a result of development of cell culture technology and cell purification technology, a large amount of adult stem cells Regenerated cells (PLA Cells - Processed Lipo Aspirate Cells) have been found to exist.

These regenerated cells are composed of adult stem cells, endothelial progenitor cells, growth factor producing cells, etc. and can be easily used for various treatments without special operations such as culture .

In particular, a large number of adult stem cells in adipose tissue are expected to play a crucial role in treating various intractable diseases and serious diseases as well as performing cosmetic or reconstructive functions required in plastic surgery field.

Therefore, in the past, various separation and extraction systems have been studied for separating and extracting the regenerated cells. In recent years, a centrifugal separation apparatus for stem cells has been developed and used in cosmetic molding and therapeutic drug development.

Such a conventional regenerative cell separation and extraction system mainly comprises an apparatus main body driven to extract stem cells, a centrifugal separator for separating the stem cells, a transfer unit connected to transfer the separated liquid, A peristaltic pump for transferring the liquid in the transfer tube, and a separation unit for sensing, controlling, transporting and sorting the transfer of the liquid, and separating and extracting the stem cell layer.

As a prior art document related to such a method of operating a regenerative cell extracting apparatus, there is a regenerative cell extracting system and a regenerating cell extracting method using the same, which are described in Korean Patent Laid-open Publication No. 10-2013-0068203.

The above-mentioned prior art invention is based on the fact that two units for separating and extracting regenerative cells from a cell tissue and a transfer system for interconnecting various storage bags are very simplified, so that they are easy to use and install, And a regenerative cell extracting method using the regenerated cell extracting system.

Another conventional prior art document is the system and method for isolating and concentrating regenerative cells from tissue, which is described in Korean Patent Application Publication No. 10-2006-0036861.

The above-mentioned conventional invention provides an automated system and method for separating and concentrating regenerating cells, for example, stem cells and progenitor cells, from adipose tissue.

However, the conventional method of operating the regenerative cell extracting apparatus has a limitation in the method of separating and extracting according to the use of the regenerative cell because it separates and extracts the regenerated cells from the adipose tissue by the centrifugal separation method, And the need for research and development on how to do so.

In addition, in the process of centrifuging the adipose tissue of the regenerating cells, it is difficult to separate the active ingredient from the adipose tissue, resulting in an extremely inefficient cell, resulting in a problem that the separation and extraction process takes a long time.

In order to solve the above problems, the present invention relates to a method of operating a device for extracting regenerative cells from adipose tissue, and more particularly, Separation and extraction of the cells can be performed easily, and the extraction time of the cells can be shortened and the regenerative cells can be efficiently collected and obtained.

The present invention relates to a method of operating an apparatus for separating and extracting regenerative cells from adipose tissue,

An input setting step of inputting an amount of fat to be input and processed into a regenerative cell extractor by a user using a touch screen of the main body and selecting and inputting an automatic and manual progress mode;

An adipose tissue washing step of introducing adipose tissue into a mixing chamber, injecting injection water such as saline, and rotating the unit of the mixing chamber to mix and wash the adipose tissue and the injection water;

An enzyme mixing step of injecting an enzyme into the washing fat in the mixing chamber and rotating the unit into which the washing fat and the enzyme have been introduced so as to mix the fat and the enzyme;

Centrifuging the mixture in a mixing chamber using a peristaltic pump and a transfer tube to a rotary container of a centrifugal separator, rotating the rotary container, and separating and concentrating the cells by centrifugation;

The peristaltic pump is operated to transfer and inject the water from the water chamber into the rotary container of the centrifugal separator through the transfer pipe and rotate the rotary container to wash and concentrate the cells, A cell washing step in which the cell washing step is performed;

A cell washing confirmation step of confirming whether or not the number of times of washing of cells set as an input to the control unit has been reached;

A cell recovery step of separating and extracting washed cells in a rotating container of a centrifugal separator to recover cells through a transfer tube;

. &Lt; / RTI >

As described above, the present invention relates to a method of operating an apparatus for extracting regenerative cells from adipose tissue, and more particularly, to an apparatus and a method for extracting regenerated cells from adipose tissue, The extraction process can be easily performed, and the extraction time of the cells can be shortened, and the regenerated cells can be efficiently collected and obtained.

FIG. 1 is a perspective view showing a regenerative cell extracting apparatus of the present invention, FIG. 2 is a flowchart showing an operation method of a regenerative cell extracting apparatus of the present invention, FIG. 3 is a flowchart showing a fat tissue washing step of the present invention, FIG. 5 is a flow chart showing the centrifugal separation step of the present invention, FIG. 6 is a flowchart showing the cell recovery step of the present invention, and FIG. Fig.
In the drawings, the same reference numerals are used to designate the same or similar components throughout the drawings.
In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear.

Hereinafter, embodiments according to the present invention will be described in detail with reference to the accompanying drawings.

Input setting step (A)

The user inputs the amount of fat to be injected and processed into the regenerative cell extractor using the touch screen 11 of the main body 10 and selects and inputs the automatic and manual progress modes.

Fat tissue washing step (B)

The fat tissue is put into the mixing chamber 20, the injection water such as saline is injected, and the unit of the mixing chamber 20 is rotated to mix and wash the fat tissue and the injection water.

The injection water transfer step (b1)

The peristaltic pump 60 is driven in accordance with the inputted driving signal so that the water for injection in the water chamber 40 is transferred to the container of the mixing chamber 20 through the transfer pipe 50 and injected.

Remaining liquid transfer step (b2)

When the remaining water in the transfer pipe is confirmed, the residual water is transferred to the container of the mixing chamber 20 through the inflow of air.

Mixed washing step (b3)

The unit of the container into which the fat and the main use water are introduced is turned upside down in the half-hoop shape so that the fat and the water are mixed and washed.

The mixture layer separation step (b4)

Allow the mixture in the vessel to separate and separate the layers according to specific gravity through the waiting time.

Wastewater transport step (b5)

According to the separated layer of the mixture in the vessel, the wastewater liquid is conveyed to the wastewater treatment unit through the transfer pipe (50).

Fat detection confirmation step (b6)

Confirm whether or not the fat component is detected in the waste liquid in the transfer pipe through the optical sensor.

Washing fat extraction step (b7)

When the fat component is detected during the transfer of the wastewater in the transfer pipe, the operation of the peristaltic pump 60 is controlled to terminate the transfer, and the extraction of the washing fat is completed.

Enzyme mixing step (C)

The enzymes are injected into the washing fat in the mixing chamber, and the washing fat and the unit into which the enzyme is introduced are rotated to mix the fat and the enzyme.

The enzyme transfer step (c1)

The peristaltic pump 60 is driven and the enzyme is transferred to the container of the mixing chamber 20 through the transfer pipe 50 to be injected.

(C2)

The pinch valve provided with the air filter is opened to introduce external air, thereby removing the sound pressure inside the conveyance pipe.

The injection water transfer step (c3)

The water for injection of the water chamber 40 is transferred to the vessel of the mixing chamber 20 through the transfer tube 50 and injected.

Mixing step (c4)

The container unit of the mixing chamber 20 into which the washing fat, the enzyme and the water for injection is put is rotated for 30 minutes so as to be mixed.

The centrifugation step (D)

The mixture in the mixing chamber 20 is transferred to the rotating container of the centrifugal separator 30 by using the peristaltic pump 60 and the transfer pipe 50 and the rotating container is rotated, To be separated and concentrated.

Mixture transfer step (d1)

The peristaltic pump 60 is driven and the mixture in the mixing chamber is transferred to the rotary container of the centrifugal separator 30 through the transfer pipe 50 to be injected.

Oil detection confirmation step (d2)

The mixture sent from the mixing chamber 20 to the centrifugal separator 30 is checked by the sensor to see if the oil is detected.

The mixture transfer confirmation step (d3)

When the oil is detected in the mixture to be transferred, the transfer channel on the mixing chamber side is shut off by using the pinch valve 70, the residual mixture in the transfer pipe is transferred to the centrifugal separator by driving the peristaltic pump 60, The pinch valve is opened to remove the negative pressure inside the transfer tube.

Rotating container rotating step (d4)

The rotating vessel is rotated in accordance with the operation signal of the centrifuge 30, the mixture in the rotating vessel is separated, the cells are concentrated, and the solution is transferred to the wastewater treatment unit.

Cell washing step (E)

The peristaltic pump 60 is operated to transfer the injection water of the water chamber 40 to the rotary container of the centrifugal separator 30 through the transfer tube 50 and to inject the rotary water to rotate the rotary container, And the wastewater liquid is transferred to the wastewater treatment unit.

Cell washing confirmation step (F)

Confirm that the number of times of washing the cells set in the control unit has reached.

Cell recovery step (G)

The washed cells in the rotating container of the centrifugal separator 30 are separated and extracted to recover the cells through the transfer tube 50.

Water transfer step (g1)

The peristaltic pump 60 is operated to transfer the injection water from the water chamber 40 to the rotary container of the centrifugal separator 30 through the transfer pipe 50 to be injected.

Cell collection step (g2)

The rotating container is repeatedly rotated and stopped to separate and detach the cells attached to the inner wall of the rotating container, and the cells are transferred to and collected by the recovery syringe.

Oil recovery step (H)

When the cell recovery step (G) is completed, the mixture in the mixing chamber is mixed and washed with water according to the mode set in the control unit of the extraction device, so that the oil is separated and extracted and transferred to the oil recovery unit .

The injection water transfer step (h1)

The water for injection in the water chamber 40 is transferred and injected into the container of the mixing chamber 20 by using the peristaltic pump 60 and the transfer pipe 50.

The remaining liquid transfer step (h2)

Mixed washing step (h3)

The unit of the container into which the mixture is introduced is turned upside down in a half-turn form so as to be mixed with the water.

Mixture layer separation step (h4)

The mixture in the vessel is allowed to separate over time according to its specific gravity.

Wastewater transport step (h5)

Oil detection confirmation step (h6)

Check whether the oil component is detected from the wastewater that is transported in the transfer pipe through the optical sensor.

The oil collecting step (h7)

When the oil component is detected in the process of transferring the wastewater in the transfer pipe, the operation of the peristaltic pump 60 is controlled to terminate the transfer of the wastewater, and the peristaltic pump 60 is operated to transfer the oil to the oil recovery unit.

The foregoing description is merely illustrative of the technical idea of the present invention, and various changes and modifications may be made by those skilled in the art without departing from the essential characteristics of the present invention.

Therefore, the embodiments disclosed in the present invention are intended to illustrate rather than limit the scope of the present invention, and the scope of the technical idea of the present invention is not limited by these embodiments.

The scope of protection of the present invention should be construed according to the following claims, and all technical ideas within the scope of equivalents should be construed as falling within the scope of the present invention.

1 is a perspective view showing a regenerative cell extracting apparatus of the present invention
2 is a flowchart showing an operation method of the regenerative cell extracting apparatus of the present invention
FIG. 3 is a flow chart showing the fat tissue washing step of the present invention
4 is a flow chart showing the enzyme mixing step of the present invention
5 is a flow chart illustrating the centrifugation step of the present invention
6 is a flowchart showing the cell recovery step of the present invention
7 is a flowchart showing the oil recovery step of the present invention
DESCRIPTION OF THE REFERENCE SYMBOLS
10: extractor body 11: touch screen
20: mixing chamber 30: centrifugal separator
40: Water chamber 50: Transfer pipe
60: Peristaltic pump 70: Pinch valve
A: Input setting step B: Fat tissue washing step
C: Enzyme mixing step D: Centrifugation step
E: Cell washing step F: Cell washing confirmation step
G: Cell recovery step H: Oil recovery step

Claims

An input setting step (A) for inputting an amount of fat to be input and processed into a regenerative cell extractor by a user using a touch screen of the main body, and selecting and inputting automatic and manual progress modes;
An adipose tissue washing step (B) for introducing adipose tissue into a mixing chamber, injecting injection water of saline, and mixing and washing the adipose tissue and the injection water;
An enzyme mixing step (C) of injecting an enzyme into the washing fat in the mixing chamber and mixing the fat and the enzyme;
A centrifugation step (D) for transferring the mixture in the mixing chamber to a rotating container of the centrifugal separator using a peristaltic pump and a transfer tube, rotating the rotating container, and separating and concentrating the cells by centrifugation;
The peritoneal pump is operated to transfer the water to the rotary chamber of the centrifugal separator through the transfer tube and to inject the water into the water chamber and rotate the rotary vessel to wash and concentrate the cells, A cell washing step (E) for allowing the cell to be washed;
A cell washing confirmation step (F) for confirming whether or not the number of times of washing of cells set as an input to the control unit has reached;
A cell recovery step (G) for separating and extracting the washed cells in the rotating container of the centrifugal separator and recovering the cells through the transfer tube;
A method for operating an apparatus for separating and extracting regenerative cells from adipose tissue,
The adipose tissue washing step (B)
(B1) for driving the peristaltic pump in accordance with the inputted driving signal so that the water for injection in the water chamber is transferred and injected into the chamber of the mixing chamber through the transfer pipe;
A remaining liquid transfer step (b2) for transferring the residual water to the vessel of the mixing chamber through the inflow of air when the remaining water in the transfer tube is confirmed;
A mixing and washing step (b3) of mixing the fat and the water and allowing the unit of the container into which the fat and the main use water are injected to swing up and down in a half-hoop shape;
A mixture layer separation step (b4) of separating the mixture in the vessel so as to separate the layers according to the specific gravity through the waiting time;
A wastewater liquid transfer step (b5) for transferring the wastewater liquid to the wastewater treatment section through the transfer tube according to the separated layer of the mixture in the vessel;
A fat detection confirmation step (b6) for confirming whether or not a fat component is detected from the waste liquid in the transfer tube through the optical sensor;
(B7), wherein the operation of the peristaltic pump is stopped when the fat component is detected during the transfer of the wastewater in the transfer pipe to finish the transfer and the extraction of the cleansing fat is completed,
The enzyme mixing step (C)
An enzyme transfer step (c1) for driving the peristaltic pump and transferring and injecting the enzyme into the container of the mixing chamber through the transfer tube;
(C2) removing the negative pressure inside the conveyance pipe by opening the pinch valve provided with the air filter to introduce outside air;
(C3) for transferring the injection water of the water chamber into the container of the mixing chamber through the transfer pipe and injecting the water;
A mixing step (c4) for allowing the container unit of the mixing chamber, into which the washing fat, the enzyme and the water for injection is put, to rotate for 30 minutes,
The centrifugation step (D)
A mixture transfer step (d1) for driving the peristaltic pump and transferring the mixture in the mixing chamber through the transfer tube to the rotary container of the centrifugal separator;
An oil detection confirmation step (d2) for confirming whether the mixture transferred from the mixing chamber to the centrifuge is detected by sensing the sensor;
When oil is detected in the mixture to be conveyed, the conveying path on the mixing chamber side is shut off by using a pinch valve, the residual mixture in the conveying pipe is transferred to the centrifugal separator by driving the peristaltic pump, and the pinch valve provided with the air filter is opened (D3) for removing the negative pressure inside the conveyance pipe;
(D4) rotating the rotary container in accordance with an operation signal of the centrifuge to separate the mixture in the rotary container to concentrate the cells and transfer the solution to the wastewater treatment part,
The cell recovery step (G)
A water transfer step (g1) for operating the peristaltic pump in the water chamber for transferring and injecting the peristaltic pump through the transfer tube to the rotary container of the centrifugal separator;
(G2) for allowing the cells attached to the inner wall of the rotary container to be separated and removed by repeating the operations of rotating and stopping the rotary container, and for transferring and collecting the cells to the recovery syringe Wherein the regeneration cell extracting apparatus is configured such that the regeneration cell extracting apparatus is constructed.

delete

The method according to claim 1,
When the cell recovery step (G) ends, according to the mode set in the control unit of the extraction device,
Further comprising an oil recovery step (H) for allowing the mixture in the mixing chamber to be mixed and washed with water to separate and extract the oil, and to transfer and collect the oil to the oil recovery unit Way.

The method according to claim 6,
The oil recovery step (H)
(H1) for transferring the water for injection in the water chamber to the container of the mixing chamber by using a peristaltic pump and a transfer pipe;
A residual liquid transfer step (h2) for transferring residual water to the vessel of the mixing chamber through air inflow when the remaining water in the transfer tube is confirmed;
A mixed washing step (h3) in which the unit of the container into which the mixture is introduced is turned upside down to mix with the water;
A mixture layer separation step (h4) for separating the mixture in the vessel through the waiting time according to the specific gravity;
A wastewater liquid transfer step (h5) for transferring the wastewater liquid to the wastewater treatment section through the transfer tube according to the separated layer of the mixture in the vessel;
An oil detection confirmation step (h6) for confirming whether or not the oil component is detected from the wastewater liquid conveyed in the conveyance pipe through the optical sensor;
An oil collecting step (h7) for controlling the operation of the peristaltic pump to terminate the transfer of the wastewater liquid and operating the peristaltic pump to transfer the oil to the oil recovery unit when the oil component is detected in the transferring process of the waste liquid in the transfer pipe;
Wherein the regeneration cell extracting apparatus comprises: