KR101723941B1 - Cosmetic composition for adsorbing and removing fine dust containing resin extracts of ardisia pusilla and bengal fig as active ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition for removing heavy metals or fine dust containing resin extracts of Ardisia pusilla and Bengal Fig as active ingredients. The cosmetic composition removes heavy metals and fine dusts on the skin and is effective in removing dead skin cells and protecting the skin.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for adsorbing and removing fine dust, which comprises an extract of coral, bamboo, and wood tree resin as an active ingredient. The present invention relates to a cosmetic composition for adsorbing and removing fine dust,

The present invention relates to a cosmetic composition for adsorbing or removing residual skin, comprising a resin extract of coral water and bengal gum wood as an active ingredient.

External pollutants such as heavy industrial dust, heavy metal particles from air masses, car exhausts, and dust from the air pollute people's skin and are considered to be major causes of skin aging and trouble.

Heavy metals are heavy metal elements such as lead, mercury, cadmium, zinc, nickel, etc., which have a specific gravity of 4 or more. These heavy metals are very dangerous because they accumulate in the body and accumulate in our body proteins, . In addition, it has been reported that a chemical reaction occurs in the air in the skin to generate harmful substances such as nitrogen oxides (NO) to cause skin inflammation, atopy, and skin troubles.

Fine dust refers to a small dust less than 10 μm in diameter that is invisible and is known to contain toxic substances such as sulfate and nitrate. These fine dusts are generated by the photochemical reaction in the air of toxic substances and heavy metals from automobile soot and factory smokes. When exposed to high concentration of fine dust environment, cough, eye sting, skin trouble, ≪ / RTI >

As a method for preventing skin troubles caused by heavy metals and fine dusts, it has been suggested that removing heavy metals and fine dusts from the skin through appropriate cleansing is most effective, but it is effective to remove heavy metals and fine dusts having strong adsorption power by simple cleansing it's difficult.

The coral tree is an evergreen tree belonging to the primrose tree of the primrose tree. It is a plant having flower which runs on a white petal distributed in Jejudo and Japan in mountain side from leaf axil. Also, the Bengalese rubber tree is an evergreen tree belonging to the nettle tree and the rubber tree. It is a large ovate, called banyan tree or banyan tree, and has sharp-pointed leaves. Many air roots come out from the thick branches or stems to the ground, Refers to a plant of the type. Although these coral and bengal rubber trees are known to be highly effective in removing fine dust in the air, there is no known effect of removing heavy metals and fine dust adsorbed on skin as a cleansing agent. Therefore, in the present invention, a new natural extract is applied to the above-mentioned necessity.

Korean Patent Publication No. 10-2010-0056239 Korean Patent Publication No. 10-2016-0000959

The present invention provides a cosmetic composition comprising a resin extract of coral water and bengal gum wood as an active ingredient. It is intended to provide a cosmetic composition which not only removes heavy metals and fine dust of the skin but also has an excellent effect on skin texture and skin texture.

An embodiment of the present invention provides a cosmetic composition for the removal of heavy metals and fine dusts containing a resin extract of coral water and bengal gum wood as an active ingredient.

As used herein, the " resin extract of coral and bamboo rubber trees " is prepared by mixing corn leaves and bengal gum tree leaves, which have been shredded by an extraction method known in the art, into an extraction solvent, extracting it, drying it, and diluting it with a solvent . The extraction solvent used for the extraction may be one or more solvents selected from the group consisting of petroleum ether, butyl acetate, ethyl acetate, trichloromethane, dichloromethane, chloroform and hexane, preferably hexane, ethyl acetate or petroleum ether But is not limited thereto. In addition, two or more different solvents may be mixed in the extraction solvent or sequentially used. The drying can be carried out naturally or by using a device, in particular a rotary vacuum concentrator and a freeze dryer. The dilution of the extract can be carried out by diluting it in a solvent, preferably Caprylic / Capric Triglyceride (MCT) or sesame oil to a certain concentration, preferably 0.1 to 20% by weight.

According to one embodiment of the present invention, the resin extract is used in an amount of 0.01 to 20% by weight, preferably 0.1 to 20% by weight, more preferably 2 to 10% by weight, most preferably 5% .

According to an embodiment of the present invention, the removal of the heavy metal or fine dust may be performed by adsorption of heavy metals or dissolution of fine dusts.

The composition not only inhibits skin damage caused by fine dusts including harmful heavy metals and / or harmful heavy metals, but also has the effect of removing fine dust including harmful heavy metals and / or harmful heavy metals adsorbed on the skin, There may be an improvement effect. The skin improvement may be any one selected from the group consisting of antioxidant, inflammation inhibition, skin hydration control, skin elasticity improvement, skin barrier recovery, skin texture improvement and skin erythema reduction.

Another embodiment of the present invention provides a cosmetic comprising the cosmetic composition for removing fine dust.

The cosmetics may have formulations of ointments, solutions, suspensions, emulsions, pastes, gels, creams, lotions, serums, soaps, surfactant-containing cleansing, oils and patches, , But is not limited thereto.

The present invention provides a new use for a cosmetic composition for removing harmful heavy metals and / or fine dusts containing the heavy metals of a composition containing a resin extract of coral water and bengal gum wood as an active ingredient. INDUSTRIAL APPLICABILITY The resin extract of the present invention can be used as an effective ingredient for the prevention and treatment of diseases caused by adsorption and removal of toxic heavy metals and / or fine dusts containing the heavy metals, improvement of exfoliation, antioxidation, suppression of inflammation, control of skin hydration, improvement of skin elasticity, And the like.

Fig. 1 shows the removal effect of heavy metals (lead and cadmium) according to the concentration of the resin extract of the coral water and bengal rubber tree of the present invention.
Fig. 2 shows the effect of decomposition of fine dust on the resin extract of the coral water and bengal gum wood according to the present invention over time.
Fig. 3 shows the effect of decomposition of active oxygen of the resin extract, alpha -tocopherol and BHT of the coral water and bengal gum wood of the present invention.
Figure 4 shows the cell activity of mouse macrophages (RAW 264.7) according to the concentration of the resin extract of the coral water and bengal rubber tree of the present invention.
Figure 5 shows the activity of luciferase in immune cells according to the concentration of the resin extract of the coral water and bengal rubber tree of the present invention.
FIGS. 6 and 7 show changes in IL-1β and TNF-α gene expression depending on the concentration of the resin extract of the coral water and the bengal rubber tree of the present invention, respectively.
Fig. 8 shows the effect of removing heavy metals from the mask containing the resin extract of the coral water and bengal gum wood of the present invention.
9 to 14 are graphs showing skin moisture content, skin oil content, skin elasticity, transdermal water loss, degree of skin texture improvement, and skin texture of the subject according to the period of use of the mask containing the resin extract of the present invention, It shows the change of degree of erythema.

Hereinafter, the present invention will be described in more detail with reference to one or more embodiments. However, these embodiments are illustrative of one or more embodiments, and the scope of the present invention is not limited to these embodiments.

1. Preparation of sample

1.1. Manufacture of resin extracts of coral and bengal rubber trees

The resin extracts of coral and bengal rubber trees were obtained using the Soxhlet extraction method commonly used in the art. Specifically, the shredded coral water or the leaves of Bengal gum wood were filled in 20% by volume of hexane or ethyl ether in Thimble, and heated at 120 ° C for reflux extraction. The heated solvent was refluxed 3 to 10 times to extract the resin from the shattered coral or bengal gum tree leaves, which was then filtered through 250 mesh and 0.5 mu m filter paper to obtain a liquid extract. Extraction dried material was obtained from the filtered liquid extract using a rotary vacuum concentrator and a freeze dryer. The extract was diluted with MCT to 10 wt%, and then filtered through 500 mesh and 0.2 mu m filter paper to obtain a liquid resin extract. As a result of carrying out a related test example of the composition according to the present invention thus formed, it was confirmed that there is no abnormality in the skin stability.

1.2. Manufacture of masks

The composition of the components for making the mask containing the composition of the present invention is shown in the following Table 1, and was manufactured by the following procedure. The A phase was heated to 70 ° C using a heater to completely dissolve the B phase. The C phase was heated to 70 ° C using a heater and mixed on the mixed A and B phases, and the mixture was stirred while being cooled. The E phase was warmed to 40 < 0 > C and mixed with the D phase. A, B, and C phases were mixed in a solution mixed with D and E phases, and stirred until the temperature reached 35 캜. F phase were mixed, and the weight was corrected. After stirring and mixing, the mixture was cooled and filtered to complete the mask. The resin extracts of the coral and bengal rubber trees were prepared by adding 5% by weight.

2. Experimental methods and results

Example 1 Confirmation of the Removal Effect of Heavy Metals by Resin Extract of Coral Water and Bengal Rubber Tree

(60ppm) and cadmium (30ppm) solution and the resin extract were mixed in the same volume in the separating funnel and reacted three times in order to confirm the effect of removing the heavy metal by the resin extract of the coral and bengal rubber trees. After three reactions, only the lead and cadmium solutions were separated and the residual heavy metal content of the liquid was measured by ICP-MS. At this time, a group reacted with MCT was used as a control group. The adsorption rate was calculated by dividing the concentration of the heavy metals and the adsorbed sample by the concentration of the control sample. The higher the adsorption rate, the more adsorbed and removed the heavy metals. The heavy metal removal rate was calculated as (1 - adsorption rate) × 100.

As a result of the analysis, it was confirmed that the resin extract of the coral water and the bengal rubber tree according to the present invention increased the heavy metal removal rate in a concentration-dependent manner (see Table 2 and FIG. 1). Therefore, it was confirmed that the heavy metals were removed by the resin extract of the coral and Bengal rubber trees.

Resin concentration (%) Lead Removal Rate (%) Cadmium removal rate (%) 0 - - 2 17.6 9.0 5 32.8 17.7 10 45.5 24.8

Example 2. Confirmation of fine dust decomposition effect by resin extract of coral water and bengal rubber tree

In order to confirm the decomposition effect of the fine dust by the resin extract of the coral and bamboo rubber trees, 1% of the PM10 collected by using the HEPA filter was mixed with the extract, and the mixture was stirred at 550 nm Was measured. At this time, a group containing only MCT was used as a control group. The fine dust permeability is calculated by dividing the transmittance of the sample containing fine dust by the transmittance of the control sample, and the higher the transmittance, the more effectively the fine dust is dissolved and decomposed. The fine dust decomposition rate was calculated as (1 - transmittance) × 100.

As a result of the analysis, it was confirmed that the decomposition rate of fine dust was increased in a time-dependent manner by the resin extract of the coral water and the bengal rubber tree according to the present invention (see Table 3 and FIG. 2). Therefore, it was confirmed that the resin extract of the coral and Bengal rubber trees effectively dissolves and decomposes the fine dust adsorbed on the skin.

Time (minutes) Fine dust degradation rate (%) Control group Test group 0 - - 5 0.07 3.36 10 0.03 26.01 15 0.05 45.83

Example 3. Determination of the effect of decomposition of active oxygen by the resin extract of coral and bengal rubber trees

To investigate the effect of the resin extracts of coral and bengal woods on the skin, we investigated whether active oxygen species, which play a major role in skin aging, are removed by the extracts of coral and bengal rubber trees.

In order to confirm the effect of decomposition of active oxygen on the resin extracts of coral and Bengali rubber trees, free radicals using 1,1-diphenyl-2-picryl hydrazyl (DPPH) The radical scavenging activity measurement method was used. 4 mL of methanol was added to the test tube, and the resin extracts of the coral and bengal rubber trees were added to each concentration. Then, 1 mL of 0.15 mM DPPH solution was added and reacted at room temperature for 30 minutes and absorbance was measured at 520 nm. Initial (Ai) and blank (Ab) without substrate and DPPH were measured, and positive control group compared α-tocopherol and BHT.

As a result of the analysis, the resin extracts of the coral and bengal rubber woods according to the present invention exhibited a higher concentration of active oxygen than that of the positive control group of? -Tocopherol and a lower concentration of active oxygen than the BHT (see Tables 4 and 3 below). Therefore, it was confirmed that the decomposition of active oxygen was promoted by the resin extract of the coral water and the bengal rubber tree.

Concentration (mg / L) 0 One 10 100 extract 0 32.3 43.2 76.4 alpha -tocopherol 0 24.7 48.6 80.7 BHT 0 28.5 40.8 68.8

Example 4. Confirmation of growth inhibition effect of immune cells by resin extract of coral and bengal rubber trees

In order to examine the effect of the resin extracts of the coral and bengal rubber trees on the improvement of skin beauty, the growth of mouse macrophages (RAW 264.7) affecting the inflammation and erythema of the skin was inhibited by the resin extracts of the coral and bengal rubber trees Respectively. Mouse immune cells were purchased from Korean Cell Line Bank (KCTC). Cell culture medium was prepared by adding 10% FBS (Gibco), 10% penicillin (100 units / mL) and streptomycin (100 g / mL) to DMEM (Gibco). The cultivation was carried out at 37 ° C, 95% humidity, 5% CO ₂ incubator conditions, and cells between 3 and 10 generations were used for the experiment. In order to examine the degree of cytotoxicity of RAW 264.7 cells treated with the resin extracts of the coral and bengal rubber trees, the extracts of the coral and bengal rubber trees were treated at the respective concentrations, The cells were then cultured and then the activity of the cells was measured.

As a result, the cell growth was not significantly changed after the treatment with the resin extract of the coral and bengal rubber trees (see Table 5 and FIG. 4, below), compared with the control (no treatment) group. Therefore, it was confirmed that the resin extracts of the coral and bengal rubber trees were not toxic to immune cells.

Time (hr) Concentration (mg / L) 0 One 10 100 0 100 100 100 100 24 99.4 97.9 96.8 97.5 48 98.4 98.7 97.5 97.4 72 99.5 97.6 98.3 97.9

Example 5 Confirmation of NF-kB Transcription Inhibitory Effect by Resin Extract of Coral and Bengal Rubber Trees

In order to examine the effect of the resin extracts of the coral and bengal rubber trees on the skin, the transcription ability of NF-kB, which is a transcription factor of cytokine which plays an important role in the immune response, is inhibited by the resin extracts of the coral and Bengal rubber trees Respectively.

NF-kB reporter analysis was performed to determine the degree of suppression of NF-kB transcriptional activity when the resin extracts of the coral and bengal rubber trees were treated with immune cells. The promoter region of the gene known to be transcribed by NF-kB was cloned into a vector containing the luciferase gene and inserted into the immune cells. NF-kB transcription-inducing LPS was treated to activate NF-kB transcriptional activity, and then the resin extracts of coral and Bengal rubber trees were treated at respective concentrations and luciferase activity was measured. The luciferase activity is determined by the presence of the IgG kappa NF- kappa B region (SEQ ID NO: 5-GGGGACTTTCC-3) oligonucleotides upstream of the minimal IL-8 promoter (positions 67 to +44), the NF- (Gibco-BRL), and the gene was inserted into the immune cells according to the reagent manual. The lipofectamine plus (Gibco-BRL) The transgenic ability of NF-kB was activated by treating LPS with the gene-inserted immune cells, treated with resin extracts of the coral and Bengal rubber trees at concentrations of 0, 1, 10 and 100 mg / L, Lt; / RTI > The cultured immune cells were directly added with 0.1 mL dissolution buffer (0.1 M HEPES, pH 7.6, 1% Triton-X, 1 mM DTT, and 2 mM EDTA) at the time of collection to collect cell lysates, The cells were centrifuged at rpm to obtain cell proteins. Protein concentration was measured using a Bradford assay (Bio-Rad Laboratories), and then 20 μg of protein was added to a luciferase assay mixture (25 mM glycylglycine, 15 mM MgSO ₄ , 1 mg / mL BSA, ATP and 1 mM D-luciferin (Analytical Luminescence Laboratory), and the luminescence was measured three times repeatedly for 20 seconds using Monolight 2010 (Analytical Luminescence Laboratory) to obtain an average value.

As a result of the analysis, the transactivity of NF-κB increased by LPS after treatment with the resin extract of the coral and bengal rubber trees decreased in a concentration-dependent manner (see Table 6 and FIG. 5).

Extract concentration (mg / L) 0 0 One 10 100 LPS - + + + + Luciferase activity (multiples) One 4.29 3.86 2.67 1.89

Example 6. Confirmation of Inflammatory Cytokine Synthesis Inhibitory Effect by Resin Extract of Coral and Bengal Rubber Trees

In order to examine the effect of the resin extracts of the coral and bengal rubber trees on the skin cosmetic improvement, it was confirmed that the synthesis of inflammatory cytokines inducing the immune response was inhibited by the resin extracts of the coral and bengal rubber trees.

In order to confirm the degree of expression of inflammatory cytokine by the resin extract of the coral and bamboo rubber trees, the LPS-treated immune cells were treated with the resin extracts of the coral and bengal rubber trees at respective concentrations and then cultured for 24 hours Respectively. The gene expression changes were then measured by qRT-PCR. In order to quantitatively determine the expression of IL-1β and TNF-α gene expression by resin extracts from coral and Bengali rubber trees, SYBR Green I (Invitrogen) was used as a method of confirming the results of the fluorescence values emitted by the PCR products Respectively. The reaction solution was prepared by mixing 0.2 μM primer, 50 mM KCl, 20 mM Tris / HCl pH 8.4, 0.8 mM dNTP, 0.5 U Extaq DNA polymerase, 3 mM MgCl ₂ and 1 × SYBR green. The denaturation, annealing and polymerization were carried out at 94 ° C for 30 seconds, 60 ° C for 30 seconds, and 72 ° C for 30 seconds, respectively, after primary denaturation at 94 ° C for 3 minutes using Linegene K (BioER) 40 cycles and fluorescence intensity was measured after each cycle. PCR results were verified by melting curve for each result. The Ct value of each gene was normalized to the Ct value of β - actin, and the change of the Ct value was compared and analyzed. The Ct value was the number of cycles when the amount of fluorescence generated by the PCR product (the number of amplified PCR products) reached a constant reference value above the basal value, and the amount of gene expression was confirmed through the Ct value. The primers of each gene used in the experiment are shown in Table 7 below. L-NG-monomethyl arginine and acetate salt (L-NG-Monomethylarginine, Acetate Salt; L-NMMA) were used as the positive control.

gene Forward primer Reverse primer beta -actin 5-CCAAGGCCAACCGCCGC-3 5-AGGGTACATGGTGCCGCC-3 IL-1? 5-CTCAATGGACAGAATATCAACCAACA-3 5-ACAGGACAGGTATAGATTCTTTCCTTTG-3 TNF-a 5-TGCCTATGTCTCAGCCTCTTC-3 5-GAGGCCATTTGGGAACTTCT-3

As a result of the analysis, the expression of IL-1β mRNA increased by LPS in a concentration-dependent manner after treatment with the resin extract of the coral and bengal rubber trees was decreased as compared with the LPS-treated group (see Table 8 and FIG. In addition, the expression of TNF-α mRNA increased by LPS in a dose-dependent manner after treatment with the resin extract of the coral and Bengali rubber trees was decreased as compared with the LPS treatment group (see Table 9 and FIG. 7).

Extract concentration (mg / L) 0 0 One 10 100 LPS - + + + + IL-1? MRNA expression
(Drainage) One 4.69 3.79 3.04 2.19

Extract concentration (mg / L) 0 0 One 10 100 LPS - + + + + Expression of TNF-α mRNA
(Drainage) One 3.99 3.84 2.52 1.67

Example 7. Confirmation of Skin Heavy Metal Removal Effect by Resin Extract of Coral Water and Bengal Rubber Tree

In order to confirm the effect of removing the heavy metals from the resin extract of coral and bengal rubber trees, pig skin (1 cm × 1 cm) was immersed in 600 ppm lead solution for 1 hour and then dried at room temperature. A common mask (a mask not containing a resin extract of coral and bengal rubber trees) was applied for 15 minutes each, and then washed with a general cleansing tissue to confirm the removal of lead. As a control group, a group which was cleaned only with a cleansing tissue without using a mask was used. The extent of lead removal was determined by measuring the amount of lead remaining on the skin after treating each washed pig skin with 20 ml of 5% nitric acid aqueous solution for 30 minutes, using ICP-MS and calculating the heavy metal removal rate according to the following formula.

Heavy metal removal rate (%) = [1 - (Heavy metal content of skin after cosmetic treatment / Heavy metal content of skin after immersion of lead solution)] × 100

As a result of analysis, a high level of heavy metal removal rate was confirmed in the mask containing the resin extract of the coral water and the bengal gum wood according to the present invention, as compared with the general mask (see Table 10 and FIG. 8). In addition, although the general consumers expected that the heavy metal and / or fine dust adsorbed on the skin would be sufficiently removed when the cleansing tissue was cleaned using the cleansing tissue, The removal rate was not high.

Lead Removal Rate (%) Control group 3.2 Coral and Bengal rubber trees
Mask 14.3 Normal mask 4.6

Example  8. A coral  And Bengal  Skin by resin extract Water content  Confirmation of index change

In order to examine the effect of the resin extract of coral and bengal rubber trees on improving skin cosmetics, skin moisture was analyzed using a corneometer and a sebumeter.

First, in order to examine the effect of the resin extract of the coral and bengal rubber trees on the improvement of the skin oiliness, 30 adult women aged 30 years or older were selected and the above-prepared mask was used. The control, positive control, and experimental groups were allowed to use masks for 2 weeks and 4 weeks, respectively, and skin moisture index was measured according to the instrument manual using a moisture analyzer (MPA5, Courage-Khazaka Electronic GmbH). Using a moisture analyzer, the same test person measured the right ball portion of the subject to be measured three times in succession. Skin oil index was measured according to the instrument manual using a sebum analyzer (MPA5, Courage-Khazaka Electronic GmbH). Using the sebum analyzer, the same person in charge of the test measured the area of the forehead which is the measurement site of the subject.

As a result, the skin moisture was increased in a time-dependent manner after treatment with the resin extract of coral and bengal rubber trees (see Table 11 and FIG. 9), as compared with the control (no treatment) group. Also, in the case of oil, the skin moisture was increased in a concentration-dependent manner after treatment with the resin extract of the coral and bengal rubber trees, as compared with the control (no treatment group) (see Table 12 and FIG.

Before experiment after 2 weeks After 4 weeks Control group 252 241 218 Positive control group 248 256 272 Experimental group 256 284 323

Before experiment after 2 weeks After 4 weeks Control group 328 322 316 Positive control group 331 344 359 Experimental group 325 364 386

Example 9. Confirmation of improvement of skin elasticity by resin extract of coral water and bengal rubber tree

The effect of improving the skin elasticity was confirmed in order to confirm the skin cosmetic improvement effect by the resin extract of the coral and bengal rubber trees. In order to examine the effect of resin extracts of coral and bengal rubber trees on skin thickness, 30 adult women aged 30 years or older were selected and the above prepared mask was used. The control, positive control, and experimental groups were allowed to use masks for 2 weeks and 4 weeks, respectively, and the extent of skin elasticity was measured with a DermaLab USB elasticity probe. The DermaLab USB elastic probe shows the skin change and resilience with time of inhalation and inhalation time. The same test person measured the probe three times after fixation to the left ball with tape.

As a result of analysis, skin elasticity increased in a time-dependent manner after treatment with resin extracts of coral and bengal rubber trees, as compared to the control (no treatment group) (see Table 13 and Fig.

Before experiment after 2 weeks After 4 weeks Control group 10.94 11.05 11.09 Positive control group 11.05 11.52 11.87 Experimental group 10.99 11.64 12.69

Example 10. Confirmation of skin barrier improvement effect by resin extract of coral water and bengal rubber tree

The skin barrier improvement effect was confirmed in order to confirm the skin cosmetic improvement effect by the resin extract of the coral and Bengali rubber trees.

First, in order to examine the effect of the resin extracts of the coral and Bengali rubber trees on the improvement of the skin barrier, 30 adult women over 30 years old were selected and the prepared mask was used. Cream and toner were used for 2 weeks and 4 weeks for the control, the positive control and the experimental group, respectively. Then, the TEWL (TransEpidermal Water Loss) was measured using a DermaLab USB TEWL probe (Cortex Technology, Inc.) It was measured according to the manual of the apparatus.

 As a result of analysis, the transdermal water loss was decreased in a time-dependent manner after treatment with the resin extract of the coral and bengal rubber trees, as compared with the control (no treatment) (see Table 14 and FIG.

Before experiment after 2 weeks After 4 weeks Control group 33.12 32.54 33.48 Positive control group 33.48 31.58 29.46 Experimental group 33.31 30.16 27.56

Example 11. Confirmation of skin texture improvement effect by resin extract of coral water and bengal rubber tree

In order to confirm the effect of the resin extract of coral and bengal rubber trees on the improvement of skin beauty, the effect of improving skin texture was confirmed.

First, in order to examine the effect of the resin extract of the coral and bengal rubber trees on the improvement of the skin texture, 30 adult women over 30 years old were selected and the prepared mask was used. After 2, 4, and 5 weeks of masking for the control, positive control, and experimental groups, the skin texture was measured using a PRIMOS light (field of view 45 × 30-simple, flexible 3D measuring, GFMesstechnik GmbH) Respectively. Using the PRIMOS light, the same test person measured the area of the forehead of the subject three times in succession and measured the same area.

As a result of analysis, the depth of wrinkles generated on the skin to determine skin texture decreased in a time-dependent manner after treatment with resin extracts of coral and bengal rubber trees, as compared to the control (untreated group) (see Tables 15 and 13 below) ).

Before experiment after 2 weeks After 4 weeks Control group 15.45 15.38 15.15 Positive control group 15.35 14.19 13.59 Experimental group 15.18 13.48 12.31

Example 12. Confirmation of erythema improvement effect by resin extract of coral water and bengal rubber tree

Through the above Examples 4, 5 and 6, the inhibition of the proliferation of the immune cells and the decrease of the expression of the inflammatory cytokines by the resin extracts of the coral and bengal rubber trees were confirmed. Thus, the effect of reducing the erythema of the resin extracts of the coral and bengal rubber trees was confirmed by analyzing whether erythema was reduced by the reduced cytokine.

First, in order to examine the effect of the resin extract of coral and bengal rubber trees on the erythema improvement, 30 adult women aged 30 years or older were selected and the prepared mask was used. The control, positive control and experimental groups were allowed to use masks for 2 weeks and 4 weeks, respectively, and then spectrophotometer CR-2600D (Konica Minolta) was used according to the instrument manual. The same test person measured the right side of the subject's right side of the ball three times in succession, and the mean value was calculated and used for the analysis. The values measured in the spectrophotometer are L *, a *, and b *, and the red value indicates a lot of red as the value of a * increases to a value indicating erythema.

As a result of analysis, erythema decreased in a time-dependent manner after treatment of the resin extract of coral and bengal rubber trees, as compared to the control (no treatment) (see Table 16 and FIG. 14, below).

Before experiment after 2 weeks After 4 weeks Control group 12.57 12.65 12.54 Positive control group 12.29 12.18 11.94 Experimental group 12.48 11.94 11.21

The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than the foregoing description, and all changes or modifications derived from the meaning and scope of the claims and equivalents thereof are included in the scope of the present invention. .

Claims (6)

A cosmetic composition for the removal of heavy metals and fine dusts, which comprises a resin extract of coral and bengal rubber trees as an active ingredient.
The method according to claim 1,
The cosmetic composition for removing heavy metals and fine dusts according to claim 1, wherein the resin extract of the coral water and the bengal rubber tree is prepared by diluting the dried product of the resin extract obtained from the leaves of the coral water and the bengal rubber tree with a solvent.
The method according to claim 1,
Wherein the resin extract is contained in an amount of 0.1 to 20% by weight based on the total weight of the composition.
The method according to claim 1,
Wherein the heavy metal and fine dust removal is performed by adsorption of heavy metals and dissolution of fine dusts.
The method according to claim 1,
The cosmetic composition for removing heavy metals and fine dusts according to any one of claims 1 to 3, wherein the cosmetic composition has antioxidant, anti-inflammatory, skin oil control, skin elasticity improvement, skin barrier recovery, skin texture improvement and skin erythema reduction.
A cosmetic comprising the heavy metal and the cosmetic composition for removing fine dust according to any one of claims 1 to 5.

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