KR101715822B1 - A disinfectant aquaculture method for fish and shellfish by using eletrolytic mixed oxidant - Google Patents

Abstract

The present invention relates to a running water fish culture method of fish and shellfish using electrolytic mixed oxidant disinfection and, more specifically, to a novel fish culture method of fish and shellfish, which can greatly reduce death of fish and shellfish caused by various pathogenic microorganisms while minimizing the cost of fish culture facilities, by disinfecting seawater and fresh water having various pathogenic microorganisms with a mixed oxidant and using the same as fish culture water of the fish and shellfish.

Description

Technical Field [0001] The present invention relates to a disinfectant aquaculture method for fish and shellfish by using an electrolytically mixed oxidant disinfection,

More particularly, the present invention relates to a method for culturing fish and shellfish using electrolytic mixed oxidant disinfection, and more particularly, to a method for disinfecting seawater and fresh water containing various pathogenic microorganisms by a mixed oxidant generated by electrolysis The present invention relates to a method for culturing a new fish and shellfish which can dramatically reduce the death of fish and shellfish caused by various pathogenic microorganisms while minimizing the cost of aquaculture facilities by using them as aquaculture farming water.

Globally, as the environment of sea and fresh water has increased due to the increase of pathogenic microorganisms due to various pollutants, about 40-50% of the dead fisheries have been seriously killed. Biosafety Aquaculture System (RAS), Biofloc, and BAS (Biosafety Aquaculure System) have been developed.

However, it is pointed out that the existing methods of farming are limited to small farms that have a lot of facilities for farming and already have facilities.

In order to solve these problems, it is necessary to sterilize 100% of pathogenic microorganisms like the existing methods of biosecurity to disinfect the water entering the aquaculture, Methods of reducing mortality by killing 90% or more of microorganisms have been studied for a long time.

As an example, a method of disinfecting all of the aquaculture (3,600 m3 / hr / 5,000 m 2) in a large number of aquaculture (3,600 m3 / hr / 5,000 m ₂ ) includes disinfection of chlorine (Cl ₂ ), disinfection of ozone, chlorine dioxide (ClO ₂ ), bleaching powder, sodium hypochlorite NaOCl) and UV (Ultra Violet) disinfection methods have been studied for over 30 years. However, the disinfection method of these sterilizing facilities and medicines cost a lot to increase the concentration of various pathogenic bacteria to more than 90% And there is no practical use because fish and shellfish (aquatic organisms) are killed due to excessive disinfecting power.

However, only UV (Ultra Violet) sterilization method has been applied. However, this method is only used for small-scale fisheries that are not exposed to natural goods such as seedling cultivation field because the sterilization power is lost when sea water and fresh water become turbid due to typhoon, have.

In addition, in order to secure the defects of UV sterilization power in the circulation type fish farm where the aquaculture quantity is small, it is sterilized by ozone, and if excess ozone is left, the ozone is destroyed by UV However, this method is limited to circulating filtration methods in which only 1-10% of the total aquaculture amount is exchanged with fresh water because of the high cost.

In some cases, ozone is injected into seawater to generate OPO (Ozone Produced Oxidant) such as HOBr, NaOBr, and Br ₂ to kill pathogenic microorganisms and to maintain a certain amount of OPO in aquaculture tank to inhibit the generation of pathogenic microorganisms However, there is a risk that the use of Bromate (BrO ₃ ), known as a carcinogenic substance, may occur in excess of 10 ppb based on WHO drinking water, and ozone generator and control device installation costs are too high to be put to practical use It is not possible.

On the other hand, there are some differences in the fish species that occur depending on the fish species, but in the case of the flounder that is most cultivated in Korea, VIR (Viral Hemorrhagic Septicema), HIRRV (Hirame Rhabdo Virus), Scuticocilliata, , Edwardsiella tarda and blight are the most common causative organisms. According to various literatures, Ct Value mg * min / L for Mixed Oxidant (MO) C: MO concentration, t: time), but in general, it is estimated that 99.9% or more is killed within 8 minutes at the concentration of 0.1-0.7 mg / L of mixed oxidant (MO).

However, pathogenic microorganisms (eg, VHS, Scuticocilliata) that do not die within 8 minutes at 0.1-0.7 mg / L MO concentration are highly resistant to mixed oxidants (MOs) (Protozoa), which are not visible to the naked eye, must exceed the minimum Ct (C: MO concentration, t: time) value of 16 (MO concentration 2 mg / In order to sterilize all of the pathogenic microorganisms so that they can be completely sterilized by the pathogenic microorganisms, it is necessary to use Korean Patent No. 10-1498990, Korean Patent No. 10-1547566, 10-1613856, which is incorporated herein by reference in its entirety.

However, in order to apply the latest biosecurity method, a plurality of chambers and a form system for neutralization are required.

Therefore, it is difficult to apply the above-mentioned water-based sterilization system in a farm where the system can not be applied. In order to apply the sterilization system, it takes a lot of equipment cost and is difficult to apply to small farmers.

Therefore, it is not possible to completely reduce the mortality caused by pathogenic microorganisms. However, in order to reduce the mortality rate and increase the income of the fishermen and reduce the amount of antibiotics used, the disinfectant aquaculture method, DAM ) Was absolutely necessary, but research and development have not been successful yet.

1. Korean Patent No. 10-1498990 2. Korean Patent No. 10-1547566 3. Korean Patent No. 10-1613856

In order to solve the above-mentioned problems, the present invention provides a method of sterilizing and neutralizing a mixed oxidant generated by electrolysis, And it is a problem to minimize the cost of fish and shellfish even at low cost by improving costly problems.

Accordingly, it is an object of the present invention to provide an economical method of fish and shellfish production that minimizes fishery mortality without excessive facilities by disinfecting the aquaculture water at low cost using mixed oxidants generated by electrolysis.

Another object of the present invention is to provide a method for sterilization and neutralization of aquaculture using a mixed oxidant sterilization method using electrolysis, And to provide a method for culturing fish and shellfish which can economically produce fish and shellfish.

Another object of the present invention is to provide a method for producing mixed oxidants at a high concentration in a storage tank in a simple and economical manner without using a neutralizing agent after sterilizing aquaculture water in a farm where a high-priced water tank is not installed or a small- And then disinfecting the whole of the aquaculture water using this to minimize the mortality of the fish and shellfish without sterilization treatment.

In order to solve the above problems, in the present invention, when seawater, fresh water or mixed water thereof is brought in and it is difficult to electrolyticize the whole aquaculture water, a part of the aquaculture water is electrolyzed to produce mixed oxidant A water supply step of introducing water into the water tank and leaving the remaining water to the water storage tank as natural water; Mixing the mixed-oxidant-containing aquaculture water and natural aquaculture water in the water tank to sterilize the mixture; Taking the sterilized aquaculture water into the aquaculture tank while controlling the concentration of the mixed oxidant in the aquaculture tank to be maintained at 0.01 to 0.09 mg / L (ppm) at all times, And a method of cultivating salted fish and shellfish using electrolytic mixed oxidative disinfection.

The present invention also relates to a water supply system for a water supply system, comprising: a water supply inlet for introducing seawater, fresh water or mixed water thereof; An oxidant generator for generating a mixed oxidant through electrolysis when a part of the aquaculture water is difficult to be electrolyzed; A water storage tank in which the number of aquaculture water passed through the oxidant generator and the remaining natural aquamarin water are mixed and stored; The electrolytic mixed anoxid sterilization including the aquaculture tank in which the mixed water is sterilized in the water tank so that the concentration of the mixed oxidant in the aquarium is maintained at 0.01 to 0.09 mg / L (ppm) The present invention provides a water-based fish and shellfish culture system using water.

According to a preferred embodiment of the present invention, the mixed oxidant may be produced to have a concentration of 0.1 to 30 mg / L.

Also, according to a preferred embodiment of the present invention, it is preferable that the disinfection treatment of the mixed water in the water storage tank is performed for 10 seconds to 8 minutes.

According to the present invention, when there is a large amount of floating matters and solids in the aquaculture, if electrolysis can be performed by filtration, a portion of the aquaculture water is electrolyzed to produce an excessive amount of mixed oxidant, As a result, it is possible to cultivate in a simple facility while minimizing the mortality of fish and shellfish, so that the fish can be economically cultured.

In addition, there is no need for expensive facilities such as chambers for the sterilization and neutralization of the aquaculture water, and the disinfection treatment necessary for the fish culture can be performed without using the neutralizing agent.

Particularly, in the general aquaculture and cage farms, about 4050% of dead plants are caused by pathogenic microorganisms. The water-based disinfection method (DAM) according to the present invention requires considerably less facility cost and maintenance cost, It is possible to kill more than 90% of pathogenic microorganisms even if it is installed simply in existing small and low-sized fish farms which can not be used for aquaculture. Therefore, the use of antibiotics is remarkably reduced to greatly contribute to the production of national health food, The income of the household is expected to increase significantly.

FIG. 1 is a configuration diagram showing a pouring type fish and shellfish culture system using electrolytic mixed oxidative disinfection according to the present invention.

Hereinafter, the present invention will be described in more detail as an embodiment.

Unlike conventional sterilization aquaculture systems, a large number of suspended solids and aquatic organisms are required to be sterilized after filtration. However, filtration of aquaculture water is not performed, and only a part of the aquaculture water is filtered, A high concentration of mixed oxidants with high sterilization power is produced at a high concentration and mixed with other natural aquatic waters to disinfect the mixed oxidant in a predetermined concentration and then used as aqua regia, And an economic system and a system for economizing the mortality rate.

The present invention is based on the fact that the mixed oxidant has a strong sterilizing power and the stability against fish is relatively high compared to other chemical bactericides, It is a method of cultivation which is used by disinfecting at a mixed oxidant concentration that can be killed.

In the present invention the term "mixed-oxidant (MO Mixed Oxidant)" also refers to a mixed oxide which occurs in number form using the electrolysis, and, for example, HOBr, HOCl, NaOBr, NaOCl, ClO _2, O _3, such as sterilization of the , Which is also called total residual oxidant (TRO), which has a strong sterilizing power and is contained in aquaculture water. This "mixed oxidant" can be produced by dissolving sea water itself in the case of sea water, and electrolyzing it in an electrolytic cell without a diaphragm by dissolving the salt water in the case of fresh water to a salt concentration of about 3%.

In the present invention, the term " disinfection " means that the "mixed oxidant" generated by electrolysis is contained at a predetermined concentration in the aquaculture water, so that more than 90% of various pathogenic bacteria contained in the aquaculture water are killed, This means a state in which disinfectant treatment is performed by a mixed oxidant treatment to such an extent that the product is hardly generated, and is distinguished from a sterilization treatment in which pathogens are completely killed. Therefore, the "disinfection process" is the disinfection of swimming pools, such as disinfection of pooled mixed aoxid is treated with a safe concentration in the human body and fish, a certain amount of water remaining in the water can be sterilized enough to prevent the death of fish and shellfish, but not harmful to the human body, Means maintaining aquaculture status with a possible mixed oxidant content.

However, the sterilization treatment applied in the conventional sterilization method means that the content of oxidant is excessive as compared with the "disinfection treatment", so that the treatment with the oxidant is carried out with the oxidant at a concentration which completely kills all pathogenic bacteria. Therefore, after the sterilization process, the sterilized water is kept in the chamber for a certain period of time to decompose the oxidant content and used as aquaculture water after the neutralization treatment is harmless to the fish and the human body and becomes a desirable condition for the fish and shellfish culture.

DISCLOSURE OF THE INVENTION In view of the fact that pathogenic microorganisms causing diseases in fish farms mainly cultured in fish farms are mostly sterilized in aquaculture water having a low concentration of oxidant, a method of disinfecting the aquaculture water by a simple method without completely sterilizing It is based on what we know.

In the present invention, it is necessary to first understand how sterilization of pathogenic bacteria against aquaculture fish at a mixed oxidant concentration is carried out in connection with disinfection treatment of aquaculture water. The following table 1 shows the results of the determination of the death rate and the possible time of death of various pathogenic microorganisms in the mixed oxidant (or the total residual oxidant: TRO).

Pathogenic microorganism name MO (TRO) concentration to be killed mg / L time
(second) Sterilization effect (%) ① Flounder Rhabdovirus (HIRRV) 1), 4) 1) 0.5
4) Low R. 30 99.9 (2) NNV (Nervous necrosis virus) 15) 1) 0.5
4) Low R. 60 99.9 ③ IPNV (infectious necrotizing virus) 1), 4) 1) 0.5
4) Low R. 60 99.9 ④ VHS 16) 4) Low R.
16) 8 -
3 minutes 99.9 ⑤ Edwardshiella tarda 2) 0.5 30 99.9 ⑥Aeromonass almonicida ATCC 14174 1)
1 minute from 14 opo 1.6 0.5 15 99.9 ⑦ Vibrio anguillarum NCMB-6 1), 2), 7) 1) 0.5
7) 0.12 60
60 99.9 ⑧Escherichia Coli 1) 1) 0.14
7) 0.1 60
120 99.9
99.999 ⑨ Streptococcus sp. YT-3 3) 0.168 60 ⑩Scuticocilliatida BR-9001 1), 4) 1) 0.8
4) High R. 30 99.9 (11) LCD Virue 4), 12) 0.1 60 99.9 ⑫VHS Virus 8) ⑬ Streptococcus Sp 2) Middle R.
0.58 10 minutes 99.9 ⑭Trichodina 4) High R. Note) 11 Note) 11 ⑮ Fly-bacterium that performs sliding movement such as Flexibacter 14)
1.6 1 minute Mucus spores collected during the flounder urinary period 4)
Parvicapsula anisocaudata (PA) 4) High R. Note) 11 Note) 11 4), 6)
Enteromyxum leei, Leptotheca fugu Japan
= Myxidium spp (Yasuda thesis) 4) High R.
6) 40 mJ / cm2 Note) 11 Note) 11 Flounder flounder
Myxobolus sp 80% same Korean literature estimate Kudoa yasunagi
Kudoa amuniensis 13) 68 -216
mJ / cm2
4) High R 10 minutes 99.9 Kudoa septempuncta 4), 5), 6) Kudoa neurophila (same as DNA97% K.S.) 5) 0.9
6) 40 mJ / cm2 10 minutes 99.9 All Bacteria 4) 0.6 1 minute All Bacteria 17) 1.4 1 minute 99.81 Rotifer 18) 1.63 10 minutes 99.99

The numbers in parentheses in Table 1 indicate the following document basis or meaning.

1) Hisae Kasai et al, 2002, HOKKAIDO Univercity

2) J. Fish. Pathol. 12 (1) 42-48 (1999)

3) 1993-1994 Ozone Year in Japan

4) J. Fish Pathology, 44 (1) 9-13, 2009, Hokkaido University, Yoshimizu and Kasai Professor

5) Journal of Fish Disease, 2013, 36, 57 65, Australia Tasmania University, Jennifer

6) Diseases of Aquatic Organism Dis Aquart Org. Vol. 74, 113 118, 2007, University of California, USA Hedrick

7) Nippon Suisan Gakkaishi, 63 (1) 97 102 (1997)

8) The Center for Food Security & Public Health, Iowa State University, 2007

9) In the case of Shimadzu Nerve Necropsy Virus (NJNNV), UV sterilization is killed at 100,000w / cm2 (100mJ / cm2), and Oxidant is killed at 0.1mg / L TRO only 150seconds.

10) High Resistance in UV sterilization refers to microorganisms killed at 100 - 500mJ / ㎠ and means microorganisms killed at less than 100mJ / ㎠ of Middle R.

11) Kudoa septempuntata and Kudoa neurophila with 97% or more DNA were reared in sterilized aquaculture at a TRO concentration of 0.9 ppm and were not infected.

12) It has become clear that LCD Virus is a virus that is vertically infected.

13) Fish Pathology, 49 (3) 141 144, 2014. 9., Seriola lalandi (yellowtail amberjack)

14) Ozonation and UV Disinfection Steven Summerfelt & Brian Vinici Fresh water Institute Shephordstown, WV

15) Journal of Fisheries Technology, 4 (1) 15 20, 2011

In the present invention, it is possible to solve the problem by considering the sterilizing power of the mixed oxidant for each pathogen as described above, and a new technology capable of achieving the object of the invention is constituted.

According to a preferred embodiment of the present invention, for example, electrolytic devices are installed in some lines of about 1-30 vol.%, Or several of the incoming lines, which are part of the number of aquaculture, to generate mixed oxidants at a high concentration The remaining 70-99 vol.% Of the aquaculture water is mixed with the water for disinfection for a predetermined time, and the sterilized aquaculture water is injected into the aquaculture water tank.

According to a preferred embodiment of the present invention, the content of mixed oxidants generated in a part of the aquaculture water is preferably 0.1 to 30 mg / L, more preferably 0.6 to 10 mg / L. If the concentration of mixed oxidants initially produced in some aquaculture is less than 0.1 mg / L, the bactericidal power becomes too weak. If the concentration is increased to a concentration higher than 30 mg / L, Bromate (BrO ₃ ), known as a carcinogenic substance, It can be overdone to 10 ppb or more, which is the water standard value.

According to a preferred embodiment of the present invention, a separate water tank is installed before the seawater and the fresh water are introduced into the aquaculture tank in a fish farm where the high-priced water tank is not installed or the high-priced water tank is small, (MO) at a high concentration in the electrolytic apparatus, for example, by supplying the electrolytic solution at a concentration of 1-30 vol% in the inlet form water or a part of several inlet lines corresponding thereto The electrolytic device is installed to generate a mixed oxidant, which is introduced into the water tank, and the remaining 70-99% by volume is supplied to the water storage tank as it is natural water.

According to a preferred embodiment of the present invention, it is preferable to mix the aquaculture water in the water tank so that the mixed oxidant concentration is 0.06-0.7 mg / L (ppm) in the state where the water is fed continuously.

In addition, according to a preferred embodiment of the present invention, it is necessary to disinfect the mixed culture water injected into the water storage tank so that the water is passed through the water storage tank for 10 seconds to 8 minutes, Was treated with disinfectant in a state where more than 90% of the pathogenic microorganisms were killed in the order of 10 seconds to 4 minutes. Then, the mixed microorganisms were administered to the aquaculture tank and the mixed oxidant concentration in the aquaculture tank was 0.01 - 0.09 mg / L (ppm), more preferably, 0.02-0.06 mg / L (ppm) is always maintained. This can greatly reduce the mortality of fish and shellfish by inhibiting the growth of pathogenic microorganisms within the aquaculture.

According to the present invention, since the mixed oxidant (MO) has a strong bactericidal action, it is very dangerous to fish and shellfish. The concentration of fish safety in aquaculture tank varies depending on the species, but in the case of flounder, 457 -463 (1996) "to be less than 0.03 mg / L.

However, according to the present invention, as a result of the test rearing from the fry (about 10 g) to the adult fish (about 1 kg) twice in about two years, it is absolutely safe even at less than 0.06 mg / L (ppm) It is safe for a few hours and if the concentration of 0.50 mg / L (ppm) for a few minutes is not observed, It is safe to fish and shellfish and can inhibit the growth of pathogenic microorganisms. If the concentration is lower than 0.01 mg / L, the disinfecting effect is greatly reduced and it is difficult to expect the effect of inhibiting the growth of pathogenic microorganisms. If the concentration is higher than 0.09 mg / L, it is difficult to secure safety for fish and shellfish. Of course, there is no problem with the safety of the fish even if it goes up to 0.5mg / L (ppm) for a few minutes instantaneously, but it is dangerous to last for more than 1 hour for a long time.

According to a preferred embodiment of the present invention, when the culture water is injected such that the concentration of mixed oxidant (MO) is 0.06-0.7 mg / L in the water tank, mixed microorganisms and organic matter in the inflowed seawater and fresh water, Oxidant reacts to decrease the concentration of mixed oxidant. When the water reaches the aquaculture tank through the main water line, it can be configured to maintain 0.01 - 0.09 mg / L in the aquaculture tank where the fish are cultured.

Mixed oxidants generated by electrolysis are oxides with strong sterilization power, but they are low in safety, so they can be decomposed spontaneously within a few minutes after the occurrence of mixed oxidants and react with various organic substances (chemical oxygen demand, COD substances) There are many things that disappear over time. In other words, even if the concentration of mixed oxidant is adjusted to 0.06 - 0.7mg / L in the water tank due to natural decomposition and reaction with organics in the aquarium during the process of flowing from the water tank to the aquaculture tank, And when it is injected into the aquaculture tank, 0.01 - 0.09 mg / L is maintained by organic matter such as feed residue in the aquaculture tank and fish excrement.

According to a preferred embodiment of the present invention, if a mixed oxidant is injected into the reservoir at a concentration higher than 0.5 mg / L, It becomes difficult to maintain the concentration of deoxidant at 0.01-0.09 mg / L.

However, it is desirable to inject more mixed oxidants when the incoming water is mixed with organic matter, mud, and muddy water. Mixed oxidants in the upper and lower limits are concentrations measured by Hach DR900, 3900 after injection.

As described above, the present invention is characterized in that, in the case of natural waters containing foreign matter, a part of the water is subjected to electrolytic treatment, and the whole wastewater is disinfected using the electrolytic treatment. The reason is that if there is solid matter such as suspended solids, muddy water, muddy water, seaweed, kelp, seaweed, etc. in aquaculture water, it can not be electrolyzed as it is in natural water and must be filtered, so only 1 - 30% It is a method of disinfecting the entire aquaculture water by generating the oxidant, thereby reducing the filtration facility and the disinfection treatment cost of the whole aquaculture water, and using it as aquaculture water by disinfection treatment.

However, in the absence of such solids and suspended solids, the entire aquaculture can be electrolyzed intact and disinfected by the same method as described above. That is, electrolysis is performed on the entire aquaculture water, but the electrolytic mixed oxidant is not sterilized to such an extent that it can be sterilized, but the electrolytic mixed oxidant is disinfected at a level capable of disinfecting, that is, It is possible to essentially include a method for electrolytic treatment.

Therefore, according to one embodiment of the present invention, seawater, fresh water or a mixed water thereof is introduced, but electrolyzed for the incoming aquaculture water to produce a mixed oxidant at a concentration of 0.06-0.7 mg / L, Supply of aquaculture; A step of retaining and disinfecting the mixed-oxidant-containing aquaculture water in the water tank; Taking the sterilized aquaculture water into the aquaculture tank while controlling the concentration of the mixed oxidant in the aquaculture tank to be maintained at 0.01 to 0.09 mg / L (ppm) at all times, And a method of cultivating salty water and fishery products using electrolytic mixed oxidative disinfection. Here, the disinfection treatment in the water tank may be carried out in such a manner that the water containing the mixed oxidant is allowed to stay for 10 seconds to 8 minutes.

1, the culture system of the present invention for applying the culture method according to the present invention includes the aquaculture water inlet 100 for introducing seawater, fresh water or mixed water thereof.

This type of water inlet unit 100 is preferably divided into two parts so that the water of the aquaculture water is flown. That is, for example, about 1-30 vol.% Corresponding to a part of the form number, or the number of forms of some of the several lead-in lines corresponding to this level is subjected to electrolysis to generate mixed- (200).

Therefore, the number of cultivated water passing through the oxidant generator 200 and the natural water of, for example, the remaining 70-99% by volume (or the inlet pipe not provided with electrolysis) are configured to be mixed in the water storage tank 300.

According to the present invention, the mixed water in the water storage tank 300 is maintained in a state of being maintained for 10 seconds to 8 minutes to sterilize the aquaculture water. Thereafter, the disinfected form number has a configuration to be fed to the aquarium 400 so that the Mixed Oxidant concentration is maintained at 0.01 to 0.09 mg / L (ppm).

In the aquaculture system according to the present invention as described above, the aquaculture water is separated and discharged from the seawater and the fresh water inlet unit 100 through two routes, and then collected in the water tank 200. The first route is a mixed oxidant The mixed passive oxidant is generated in the range of 0.1 to 30 mg / L, more preferably 0.6 to 10 mg / L, and is sent to the water storage tank 300, and the second route The incoming form water quantity is introduced into the water storage tank 300 in a natural state and mixed. Thereafter, the mixed form water is kept in the water tank 300 for a certain period of time while being mixed with 0.06-0.7 mg / L of mixed oxidant, and then sterilized and finally injected into the water tank 400. At this time, the mixed aoxidant content in the aquarium 400 is injected so as to maintain 0.01-0.09 mg / L.

When using the electrolyzed mixed oxidant disinfecting method and the aquaculture system using the electrolytic method according to the present invention, the carcinogenic substance, Bromate, exhibits a favorable effect that does not exceed the WHO drinking water standard.

Further, according to the present invention, since the cost of the Ozone OPO generating device and the control cost of each device can be greatly reduced by about 1/2 to 1/3 or less, it is possible to economically and efficiently form fish and shellfish, It can be practically used as a method of cultivation.

In particular, in the case of the conventional sterilization method, a plurality of chambers are required for sterilization and neutralization. However, since the present invention does not require such a chamber configuration, it is advantageous that the system can be constructed with simple facilities.

In addition, in the conventional sterilization method, it is necessary to use a neutralizing agent for the neutralization treatment so as not to affect the fish and shellfish after the sterilization using the oxidant. However, the disinfection- .

According to a preferred embodiment of the present invention, in the aquaculture tank, the concentration of mixed oxidant (MO) varies depending on the state of the fish in the aquaculture tank, the bottom biofilm, the feed residue, etc. Therefore, Measuring the ORP (Oxidation Reduction Potential) in the line, automatically adjusting the concentration of MO injected into the reservoir or measuring the MO of the aquarium or sub-line using an MO concentration meter such as Hach CLX17, It can be adjusted automatically.

Therefore, in the aquaculture system according to the present invention, the water tank or the aquarium may further include an apparatus for controlling the amount of the aquaculture water by automatically adjusting the concentration of the mixed oxidant.

Unlike the existing sterilization and neutralization treatment methods, the above-described culture method and culture system of the present invention can be applied to aquaculture, It is a technology that can produce fish and shellfish form by minimizing the mortality rate by simple and economical disinfection of the aquaculture water by diluting to the total aquaculture water by generating mixed oxidant in only the water electrolysis method and staying in the water tank.

Therefore, it is expected that it will be effectively used for practical use in small-scale micro-farms without excessive facilities and facilities.

Hereinafter, the present invention will be described in detail with reference to examples, but the present invention is not limited to the examples.

Example

2,000 flounders of 300-500g size were placed in the aquaculture tank (quantity of 70m3), 10m in height, 0.7m in height and 1.2m in wall height, respectively.

The supply of aquaculture for aquaculture was carried out by injecting 1.2 mg / L of mixed oxidant from an electrolytic cell into a water tank (2 x 2 m) for about 20% of aquaculture water, mg / L in the water tank, and then poured into the aquaculture tank. In the aquaculture, the content of mixed oxidants in the aquaculture was 0.03 - 0.08 mg / L.

The test group was set such that the concentration of mixed oxidant in the aquaculture water was automatically controlled by PLC (Programmable Logic Controller), and the control group was fed with general seawater without sterilization, , Edwards, and blight were reduced to 185 (9.25%) and 825 (41.25%) of the control group, respectively.

The experimental results are shown in Table 2 below.

Item Test section (invention section) Control Mortality rate 9.25% 41.25% Tricodina parasite Results of body surface microscopic observation
Not found Found Parvicapsula anisocaudata Anesthesia, swab from bile, not observed during microscopic examination Observed Sliding bacteria Observed but low in motility Motility is active Scutica Observed but low mobility Motility is active

Experimental Example (total viable cell count test)

The total viable cell count including the pathogenic microorganisms was tested and observed under the conditions of the test zone as described in Example 1 above and the control water using the aquaculture water and untreated natural sea water as follows and the sterilization rate was as shown in Table 3 .

In addition, Streptococcus parauberis, Edwardsiella tarda, and Vibrio spp. Were not observed in fish.

[General (total) bacterial count in seawater]

The number of total bacteria in the aquaculture was measured using a standard plate method. The medium used was selected from BHIA (Brain Heart Infusion Agar), TCBS (Thiosulfate Citrate Bile Sucrose Agar) and SS (Salmonella Shigella Agar) Respectively.

The number of viable bacteria in the specimen was counted by counting the number of bacterial colonies that occurred after culture by mixing and solidifying the specimen in the agar medium.

(1) Test operation

Aseptically collected seawater sample solutions are diluted with 10 times, 100 times, and 1000 times diluted solutions. 1 ml of aseptically collected seawater is diluted 10 times with sterile physiological saline, and 2 ml of agar medium is aseptically taken from each diluted solution. 15 ml of agar medium maintained at 43 to 45 ° C is aseptically Mix well and carefully mix and coagulate so that it does not stick to the Petri dish lid.

To prevent the spread of colonization, 3 ~ 5 ml of agar medium is added again and the coagulated medium is incubated at 25 ℃ for 24 ~ 48 hours.

(2) Estimation of the number of colonies

Calculate the number of colonies generated immediately after incubation. In principle, the number of colonies should be calculated by selecting a medium that produces 30 to 300 colonies per agar medium. If less than 30 colonies are obtained on the whole medium, the lowest dilution factor should be measured.

(3) A description of the number of bacteria

Record the number of bacteria in 1 ml of sample in the standard plate method. The number of colonies in one culture medium is multiplied by a dilution factor corresponding to the number of colonies counted, and the numerical value is the number of bacteria in 1 ml in the standard plate method.

The results of measurement of total viable cell counts as described above are shown in Table 3 below.

Item Control (natural sea water) Test area (number of sterilization form) Sterilization rate Total number of bacteria 4,300 cfu / ml 13 cfu / ml 99.697% Streptococcus Observed No colonies visible Edward I Observed No colonies visible

100 - Water supply entrance
200 - Oxidant Generating Unit
300 - water tank
400 - Aquaculture tank

Claims (9)

Water supply step of introducing seawater, fresh water or a mixed water thereof into the water tank by electrolyzing only 1-30 volume% of the total number of cultivated water and introducing mixed oxidant into the water tank,
Mixing the mixed-oxidant-containing aquaculture water and natural aquaculture water in the water tank with the mixed aoxid in a concentration of 0.06-0.5 mg / L in aquaculture water; And
Taking the sterilized aquaculture water into the aquaculture tank while adjusting the concentration of the mixed oxidant in the aquaculture tank to maintain the concentration of 0.01 to 0.09 mg /
, And the neutralization treatment is not performed, and the electrolyzed mixed oxidized disinfection is performed.
[4] The method of claim 1, wherein the mixed oxidant generated before entering the water storage tank is produced to be 0.1 - 30 mg / L.
delete [6] The method according to claim 1, wherein the disinfecting treatment in the water storage tank is performed so as to stagnate the mixed water containing mixed oxidant for 10 seconds to 8 minutes.
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