KR101707474B1 - Integrated valve unit and nucleated cell extraction device using the same - Google Patents

Abstract

The present invention relates to an integrated valve unit capable of extracting nucleated cells in a tissue extract by centrifuging the tissue extract, and a nucleated cell extracting apparatus using the integrated valve unit.
To this end, the integrated valve unit according to the present invention comprises a hollow main pipe, a raw solution pack for storing the tissue extract, an enzyme pack for storing the degrading enzyme for decomposing the tissue in the tissue extract, A plurality of sub-pipelines for connecting the main piping and the sub-piping to the main piping, a washing pack for storing the washing solution mixed with the pack, the tissue extract or the nucleated cells, and the waste solution pack for storing the foreign substance separated from the tissue extract, And an on-off valve for individually opening and closing the sub-piping supported by the fixed block and the fixed block.
In addition, the apparatus for extracting nucleated cells according to the present invention comprises the aforementioned integrated valve unit, an extraction unit for extracting nucleated cells from the tissue extract, a main body to which the integral valve unit and the extraction unit are coupled, An inlet / outlet flow passage for connecting the main pipe and the extraction unit, an inlet / outlet valve for opening / closing the outlet flow passage, a circulation pump for moving the fluid in the outlet flow passage, And a control unit for controlling the control unit.

Description

[0001] INTEGRATED VALVE UNIT AND NUCLEATED CELL EXTRACTION DEVICE USING THE SAME [0002]

The present invention relates to an integrated valve unit and a nucleated cell extracting apparatus using the same, more specifically, to an integrated valve unit capable of extracting nucleated cells from a tissue extract by centrifuging the tissue extract and a nucleated cell extracting apparatus using the same will be.

For example, the blood collected from an animal or a human body can be used for a variety of medical purposes such as blood transfusion for a surgical patient, clinical trial of a patient, and a variety of medicinal uses such as blood, Only platelets can be collected and used. Similarly, only adult stem cells can be collected and used in tissue exudates collected from animals or human bodies.

As such, specific nucleated cells suitable for the purpose can be collected and used from tissue extracts including blood. Such operations are generally performed by centrifugation.

Meanwhile, in order to obtain a specific nucleated cell, the tissue extract may be centrifuged as it is. However, in order to control the yield or acquisition time of a specific nucleated cell, a case in which a protease capable of reacting chemically or biochemically with the tissue extract is mixed There is also. In this case, the degrading enzyme which is mixed with the tissue extract and the tissue extract can be agitated through the agitating module, and the agitated tissue extract and the degrading enzyme will provide the nucleated cells of higher purity through the subsequent centrifugation.

Korean Unexamined Patent Publication No. 1216011 (entitled: Centrifugal Assembly, December 27, 2012)

It is an object of the present invention to provide an integrated valve unit capable of extracting nucleated cells from a tissue extract by centrifuging the tissue extract and a nucleated cell extracting apparatus using the integrated valve unit.

According to a preferred embodiment of the present invention, the integral valve unit according to the present invention includes a hollow main pipe; An enzyme pack for storing a degrading enzyme for decomposing tissue in a tissue extract, an extraction pack for storing nucleated cells separated from the tissue, and a washing solution for mixing the tissue extract or nucleated cells And a plurality of sub-pipelines for connecting a waste solution pack for storing foreign matter separated from the tissue extract to the main piping, respectively; A fixed block in which the main pipe and the sub pipe are inserted and supported; And an opening / closing valve for individually opening and closing the sub-pipe supported by the fixed block, wherein the fixed block has a cylindrical shape, and any one of the main pipe and the sub- And the other of the main pipe and the sub pipe surrounds the fixed block and connects the waste liquid pack to the sub pipe disposed at one side edge of the main pipe among the plurality of sub pipes, And the sub-pipe is connected to the sub-pipe adjacent to the sub-pipe.

The apparatus may further include a fixed body detachably fixing the fixed block, wherein the on-off valve includes: a cylinder coupled to the fixed body; And a piston portion reciprocally coupled to the cylinder portion to press the sub-pipe.

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The apparatus for extracting nucleated cells according to the present invention comprises an integrated valve unit according to the present invention; An extraction unit having a first inlet pipe connected to the other end of the main pipe for extracting the nucleated cells from the tissue extract and a second inlet pipe connected to one end of the main pipe; A main body to which the integral valve unit and the extraction unit are coupled; A rotation driving unit for rotating the extraction unit; A first inlet / outlet flow path for connecting the other end of the main pipe to the first inlet / outlet pipe; and a second inlet / outlet flow path for connecting one end of the main pipe to the second outlet / outlet pipe; A first inlet / outlet valve for opening / closing the first inlet / outlet flow passage, and a second inlet / outlet valve for opening / closing the second entrance / exit flow passage; A circulation pump for moving the fluid in the outflow channel portion; And a control unit for controlling the open / close valve, the rotation driving unit, the inlet valve, and the circulation pump in cooperation with each other.

Here, the apparatus further includes an active light portion for irradiating light to at least one of the sub-pipe, the extraction pack, and the outflow channel portion.

Here, the active light portion is irradiated with light having a wavelength corresponding to red light or near-infrared light.

The extraction unit includes a first inlet / outlet pipe connected to the main pipe via the inlet / outlet flow path in a state detachably coupled to the main body, wherein a first flow path is formed, and a second inlet / An inlet pipe member including a second inlet pipe forming a second flow path; A collection part fixed to the first outlet pipe so as to face the second outlet pipe and having a concave surface facing the second outlet pipe; A collecting member including an inducing portion protruding from a collecting portion; And a plurality of nuclide separation grooves formed in the outer periphery of the accommodating portion, the accommodating portion being rotated by the rotation driving unit in a state in which the entrance and exit tube members are rotatably inserted, A bottom inclined portion inclining downward from a bottom edge of the accommodating portion toward a center of the bottom of the accommodating portion; a collecting groove recessed and formed at the bottom center of the accommodating portion so as to insert an end portion of the first access pipe; And an extracting container having an upward inclining portion inclined upward toward an edge of the collecting portion.

Here, the nucleation-separation groove is formed in a cone shape whose diameter decreases from the inlet toward the inside.

According to the integrated valve unit and the apparatus for extracting nucleated cells using the same according to the present invention, nucleated cells can be extracted from the tissue extract by centrifuging the tissue extract. Particularly, it is possible to simplify the movement path of the valve and the fluid, reduce the moving distance of the fluid, and prevent deformation of the tissue extract.

Further, the present invention can stably fix the main piping and the sub piping through the integrated valve unit, improve the open / close accuracy of the sub piping, and prevent the sub piping from being broken due to the operation of the opening / closing valve.

Further, according to the present invention, the main piping in which a plurality of sub-pipes are branched can be kept clean through the integrated valve unit, and foreign matter can be prevented from mixing with the fluid supplied to the extraction unit in the movement of the fluid.

In addition, the present invention can centrifuge the tissue extract with one extracting unit through the detailed structure of the nucleated cell extracting apparatus, and automation can be realized while maintaining airtightness from the tissue extract to the extraction of nucleated cells throughout the whole process.

In addition, the present invention can activate the nucleated cells that are tired by centrifugation through the active light segment and increase the regeneration efficiency of the nucleated cells.

In addition, the present invention promotes the activation of the nucleated cells through the activated light part, and the nucleated cells extracted by the nucleated cell extracting device can be administered immediately.

In addition, the present invention can increase extraction efficiency of nucleated cells while performing both washing and decomposition and extraction of tissue extract through one extraction unit.

Further, the present invention can concentrate the collection of nucleated cells in the extraction container through the detailed configuration of the extraction unit, and suppress or prevent the mixing of the nucleated cells and the foreign substance.

1 is a view showing a nucleated cell extracting apparatus according to an embodiment of the present invention.
2 is a perspective view showing an integrated valve unit in a nucleated cell extracting apparatus according to an embodiment of the present invention.
3 is a cross-sectional view showing the coupled state of FIG.
4 is a perspective view illustrating an extraction unit in a nucleated cell extracting apparatus according to an embodiment of the present invention.
5 and 6 are sectional views showing the coupled state of FIG.
FIG. 7 is a diagram showing a connection state of a control unit in a nucleated cell extracting apparatus according to an embodiment of the present invention. FIG.
FIG. 8 is a view showing a use state of the collecting member in the apparatus for extracting nucleated cells according to an embodiment of the present invention. FIG.

Hereinafter, an integrated valve unit according to the present invention and an apparatus for extracting nucleated cells using the same will be described with reference to the accompanying drawings. Here, the present invention is not limited or limited by the examples. Further, in describing the present invention, a detailed description of well-known functions or constructions may be omitted for clarity of the present invention.

2 is a perspective view illustrating an integrated valve unit in a nucleated cell extracting apparatus according to an embodiment of the present invention. FIG. 3 is a cross- FIG. 4 is a perspective view showing the extraction unit in the apparatus for extracting nucleated cells according to an embodiment of the present invention, FIGS. 5 and 6 are sectional views showing the coupled state of FIG. 4 And FIG. 7 is a view illustrating a connection state of a control unit in the apparatus for extracting nucleated cells according to an embodiment of the present invention. FIG. 8 is a view showing the state of use of collecting members in the apparatus for extracting nucleated cells according to an embodiment of the present invention. Fig.

1 to 8, an integrated valve unit according to an embodiment of the present invention includes an integrated valve unit 10 applied to a nucleated cell extracting apparatus, while explaining a nucleated cell extracting apparatus according to an embodiment of the present invention I will explain.

The apparatus for extracting nucleated cells according to an embodiment of the present invention extracts nucleated cells from a tissue extract by centrifuging the tissue extract and comprises an integrated valve unit 10, an extraction unit 20, a main body 30, , A rotation driving section (M), an outflow channel section, an inlet valve, a circulation pump, and a control section (C).

The integrated valve unit 10 supplies the tissue extract, the degrading enzyme, the washing solution and the like to the extraction unit 20 and extracts the foreign matter containing the nucleated cells discharged from the extraction unit 20 or the tissue extract And the like.

The integrated valve unit 10 may include a main pipe 11, a sub pipe 12, a fixed block 13, and an on-off valve 14. [

The main pipe 11 is a hollow pipe.

A plurality of sub-pipes (12) are branched from the main pipe (11).

The sub-piping 12 includes an undiluted solution pack G1 for storing the tissue extract, an enzyme pack G2 for storing the degradation enzyme for decomposing the tissue in the tissue extract, A washing pack G4 for storing a washing solution mixed with a tissue extract or nucleated cells and a waste solution pack G5 for storing a foreign substance separated from the tissue extract are connected to the main pipeline 11 respectively.

At this time, a waste liquid pack G5 is connected to the sub-pipe 12 at one side edge of the plurality of sub-pipes 12 as shown in Fig. 2, and a sub-pipe 12, the washing pack G4 is connected.

Then, the cleaning liquid passes through all the main piping 11, so that all the fluids remaining in the main piping 11 are cleaned by the cleaning liquid. Therefore, it is possible to keep the main piping 11 clean at all times, and to prevent foreign matter from being finally mixed into the nucleated cells recovered by the extraction pack G3.

2, the enzyme pack G2 is connected to the sub-pipe 12 located next to the washing pack G4, and the raw-liquid pack G1 is located adjacent to the enzyme pack G2 To the sub-pipe (12). Here, although the position of the stock solution pack G1 and the enzyme pack G2 is not limited, the decomposition enzyme may be discharged to a portion where the tissue extract is discharged, The extract 12 is located behind the sub-piping 12 to which the stock solution pack G1 is connected so that the tissue extract remaining in the main piping 11 can be supplied to the extraction unit 20 together with the washing solution or the decomposing enzyme. have.

In addition, the sub-pipe 12 to which the cleaning pack G4 is connected in the direction of discharge of the tissue extract as the cleaning solution is discharged to the portion where the tissue extract and the degrading enzyme are discharged is connected to the sub-pipe 12 to which the enzyme pack G2 is connected, So that it is possible to supply all of the decomposition enzymes remaining in the main piping 11 to the extraction unit 20.

The extraction pack G3 may be connected to the sub-pipe 12 at the other side of the plurality of sub-pipes 12 as shown in FIG. Then, the extraction pack G3, the raw liquid pack G1, the enzyme pack G2, the cleaning pack G4, and the waste solution pack G5 are successively connected to the plurality of sub-pipes 12 .

The extraction pack G3 can be connected to the sub-pipe 12 between the sub-pipe 12 to which the enzyme pack G2 is connected and the sub-pipe 12 to which the cleaning pack G4 is connected. Then, the raw liquid pack G1, the enzyme pack G2, the extraction pack G3, the cleaning pack G4, and the waste liquid pack G5 are sequentially connected to the plurality of sub-pipes 12 .

Thus, the cleaning liquid is supplied to both sides of the main pipe 11, and the fluid remaining in the main pipe 11 can be supplied to the extraction unit 20.

The main pipe (11) and the sub pipe (12) are inserted and supported in the fixed block (13). At this time, a support groove into which the main pipe 11 and the sub pipe 12 are inserted is formed in the fixed block 13. The main piping 11 and the sub piping 12 are inserted into the support grooves to prevent the main piping 11 and the sub piping 12 from being damaged by an external impact, (11) and the sub-pipe (12) can be safely protected.

Since the fixed block 13 is formed in a cylindrical shape, any one of the main pipe 11 and the sub pipe 12 is disposed in the longitudinal direction of the fixed block 13, and the main pipe 11) and the sub-pipe (12) are arranged to surround the fixed block (13). This improves the straightness of the main piping 11 or the sub piping 12 so that the fluid is stagnated in the main piping 11 or the sub piping 12 due to the bending of the piping 12, Can be prevented.

3, the main pipe 11 is disposed in the longitudinal direction of the fixed block 13, and the sub pipe 12 is connected to the fixed block 13 Respectively. Thereby, it is possible to improve the straightness of the main pipe 11, simplify the arrangement of the opening / closing valve 14, make the integral valve unit 10 more compact, .

The on-off valve 14 individually opens and closes the sub-piping 12 supported by the fixed block 13. The open / close valve 14 is provided with a source liquid valve V11 for opening and closing a sub pipe 12 to which the raw liquid pack G1 is connected, an enzyme valve V12 for opening / closing a sub pipe 12 to which the enzyme pack G2 is connected, An extraction valve V13 for opening and closing a sub-pipe 12 to which the extraction pack G3 is connected, a cleaning valve V14 for opening and closing a sub-pipe 12 to which the cleaning pack G4 is connected, And a waste liquid valve V15 for opening and closing the sub-pipe 12 to which the pack G5 is connected.

The integral valve unit 10 may further include a fixed body 15.

The fixed body (15) detachably fixes the fixed block (13). The fixed block 13 is detachably fixed to the fixed body 15 through various attachment and detachment means.

The main body pipe 15 and the sub pipe 12 are connected to each other by a fixing block 13. The fixing block 15 is formed with a mounting groove so that a part of the fixing block 13 is inserted, It is possible to suppress or prevent the fluid from flowing, and to improve the operation precision of the opening / closing valve 14. [

At this time, the on-off valve 14 may include a cylinder portion 14a and a piston portion 14b.

The cylinder portion 14a is coupled to the fixed body 15 in correspondence with the sub-pipe 12.

The piston portion 14b is reciprocally coupled to the cylinder portion 14a so as to press each of the sub-pipes 12 individually. The piston portion 14b reciprocates in the cylinder portion 14a by hydraulic pressure or an applied power source.

The cylinder portion 14a of each of the on-off valves 14 is detachably fixed to the fixed body 15, and the reciprocating movement distance of the piston portion 14b from each cylinder portion 14a The respective on-off valves 14 can press the respective sub-pipes 12 equally.

The extraction unit 20 extracts the nucleated cells from the tissue extract. The extraction unit 20 includes an inlet pipe member 21, a collecting member 23, and an extraction container 25.

The outlet pipe member 21 is detachably coupled to the main body 30 and is connected to the main pipe 11 via the inlet / outlet flow path. The outlet pipe member 21 includes a first outlet pipe 211 in which the first flow path 212 is formed and a second outlet pipe 211 surrounding the first outlet pipe 211, An entrance pipe 213 is included.

The first outlet pipe 211 is connected to the sub pipe 12 side (the other end of the main pipe 11) to which the stock solution pack G1 is connected via the outlet flow path, The pipe 213 is connected to the sub pipe 12 side (one end of the main pipe 11) to which the waste liquid pack G5 is connected via the outflow channel.

The first outlet pipe 211 and the second outlet pipe 213 are arranged so as to be concentric with the rotation center of the extraction container 25 while forming independent flow paths. One end of the first inlet / outlet pipe 211 is connected to the first inlet / outlet flow channel 41, and the other end of the first inlet / outlet pipe 211 is formed so as to be adjacent to the bottom of the extraction vessel 25. One end of the second outlet pipe 213 is connected to a second outlet flow path 42 and the other end of the second outlet pipe 213 is connected to the upper end of the collecting member 23 Respectively.

The collecting member 23 is fixed to the first outlet pipe 211 so as to face the second outlet pipe 213. The collecting member 23 guides the fluid received in the receiving portion 261 to the second outlet pipe 213. By applying the collecting member 23, foreign substances contained in the receiving portion 261 can be discharged to the second inlet / outlet pipe 213 while the extraction container 25 rotates.

The collecting member 23 may further include a collecting portion 231 and an inducing portion 232 and may further include a through hole 233.

The collector 231 is formed with a concave surface facing the second outlet pipe 213. The collecting part 231 is formed in a concave disc shape and is fixed in a state in which the first outlet pipe 211 passes through the center.

The collecting part 231 is arranged adjacent to the lower end of the second outlet pipe 213 at the upper part of the extraction container 25. The edge of the collecting part 231 is set to a size adjacent to the inner peripheral surface of the extraction container 25.

The guide part 232 is protruded from the collecting part 231 so as to extend from the edge of the collecting part 231 toward the first outlet pipe 211. A plurality of the guide portions 232 are spaced apart from each other in the circumferential direction of the collecting portion 231. In one embodiment of the present invention, the guide portions 232 are spaced apart from each other at intervals of 90 degrees.

The guide portion 232 is formed on a surface facing the lower end of the second outlet pipe 213. The guide part 232 has a predetermined curvature and is formed to be long from the edge of the collecting part 231 toward the first exit pipe 211 so that foreign substances can be introduced into the extraction pipe 25 from the second exit pipe (213), and the foreign matter can be transmitted to the second outlet pipe (213) by lift.

A plurality of the through holes 233 are spaced apart from each other and are formed in the vicinity of the center of the collecting portion 231. The through hole 233 disperses the pressure applied to the collecting part 231 and prevents foreign matter from overflowing when a large amount of foreign matter is discharged to the second outlet pipe 213 .

The extraction vessel (25) is rotated by the rotation drive unit (M) in a state in which the entrance and exit tube member (21) is rotatably inserted.

The extraction container 25 is a hollow container for forming a receiving portion 261 in which the tissue extract is received and includes an extraction body 26 in which the receiving portion 261 is formed, And an extraction plug 28 coupled to the extraction body 26. Here, the coupling structure of the extraction body 26 and the extraction plug 28 is not limited, and the extraction plug 28 can open and close the receiving portion 261 by mutually coupling through various forms.

The extraction body 26 includes a nucleus separation groove 262, a bottom inclined portion 263, a collecting groove 264, and a discharge inclination portion 265.

The nucleation separation groove 262 is recessed outward from the periphery of the accommodating portion 261. The extraction nipple 262 protrudes outside the extraction body 26 so that the extraction vessel 25 can be stably fixed to the main body 30.

The nuclide isolation groove 262 is a portion where nucleated cells generated by centrifugation are collected. The nuclide isolation groove 262 has a cone shape in which the diameter decreases from the entrance to the inside, thereby increasing the collection amount of the nucleated cells, facilitating the separation of the nuclide cells from foreign substances or nucleated cells and the washing solution, A relatively light foreign substance or washing liquid can be smoothly discharged from the nucleation-separating groove 262, and the extraction efficiency of the nucleated cells can be increased.

The bottom inclined portion 263 is inclined downward from the bottom edge of the accommodating portion 261 toward the bottom center of the accommodating portion 261. The bottom inclined portion 263 can quickly collect the fluid received in the receiving portion 261 to the bottom center of the receiving portion 261. [

The bottom inclined portion 263 may be configured to lift the fluid received in the receiving portion 261 toward the periphery of the receiving portion 261 and to receive the fluid in the receiving portion 261 when the extraction container 25 is rotated It is possible to smoothly perform washing, decomposition or centrifugal separation of the fluid.

The collecting groove 264 is recessed at the center of the bottom of the receiving portion 261. The collecting groove 264 is connected to the bottom inclined portion 263, and the end of the first inlet / outlet pipe 211 is inserted in a spaced apart position.

5 and 6 as well as a slit (not shown) in the first inlet / outlet pipe 211 is formed in the form in which the end of the first outlet pipe 211 is inserted in the state of being separated from the collecting groove 264. [ And includes a recessed structure. Here, the end of the first outlet pipe 211 may be inserted into and supported by the collecting groove 264.

Accordingly, the fluid accommodated in the accommodating portion 261 is stably discharged through the first inlet / outlet pipe 211.

The discharge inclined portion 265 is inclined upward toward the edge of the collecting portion 231 around the receiving portion 261. The discharge inclination part 265 prevents the fluid contained in the accommodation part 261 from being stagnated around the accommodation part 261 in the centrifugal separation of the tissue extract, The relatively light foreign matter among the fluids can be quickly transferred to the collecting part 231. [

The outlet pipe member (21) is inserted through the extraction stopper (28). The extraction stopper 28 is provided with a support protrusion 281 protruding to be rotatably supported by the outlet pipe member 21 to suppress or prevent the flow of the extraction container 25 when the extraction container 25 rotates And the concentricity of the outlet pipe member 21 and the extraction container 25 can be maintained.

Is sealed between the extraction vessel (25) and the outlet tube member (21) so that the inside of the extraction vessel (25) is sealed. Thus, by adopting the intermediate member 215 and the rotation support member 22 in the embodiment of the present invention, the extraction vessel 25 is rotated relative to the outlet tube member 21, Can be sealed.

For example, the rotation support member 22 surrounds and supports the support protrusion 281, and the intermediate member 215 supports an outer circumferential surface of the second outlet pipe 213 to surround the rotation support member 22, As shown in Fig.

Here, the coupling structure between the extraction container 25, the rotation support member 22, the intermediate member 215, and the inlet pipe member 21 is not limited, and may be mutually coupled through various shapes, The receiving portion 261 can be sealed while the receiving portion 25 is rotated.

The main body 30 is coupled to the integral valve unit 10 and the extraction unit 20.

Although not shown, the main body 30 is provided with a first fixing part in which the integral valve unit 10 is inserted and supported, a second fixing part in which the extraction container 25 is inserted and supported, An extraction support for supporting a sub-pipe 12 to which the extraction pack G3 is connected; an extraction support for supporting the solution pack G1 and the enzyme pack G2; And a pack support portion in which the extraction pack G3, the cleaning pack G4, and the waste solution pack G5 are respectively supported.

In addition, the main body 30 may be formed integrally with the fixed body 15.

The rotation driving unit M rotates the extraction unit 20. The rotation driving unit M can rotate the extraction container 25 inserted and supported by the second fixing unit by rotating the second fixing unit. The rotation driving unit M may be rotated in the forward and reverse directions depending on the fluid received in the extraction vessel 25, and may be rotated at a high speed in one direction. Here, the rotation driving unit M is not limited, and the extraction unit 20 can be rotated through various forms.

The outflow channel connects the main pipe (11) and the extraction unit (20). The outflow channel portion may include a first outflow channel portion 41 and a second outflow channel portion 42. The first inlet and outlet flow path 41 connects the first outlet pipe 211 and the other end of the main pipe 11 and the second outlet pipe 42 connects the second outlet pipe 213, And one end of the main pipe (11).

The inlet / outlet valve opens / closes the inlet / outlet flow path portion. The inlet valve may include a first inlet valve V21 and a second inlet valve V22. The first inlet valve V21 opens and closes the first inlet and outlet flow passage 41 and the second inlet valve V22 opens and closes the second inlet and outlet flow passage 42. [

The circulation pump moves the fluid in the outflow channel portion. The circulation pump may include only the first circulation pump P1 or may include both the first circulation pump P1 and the second circulation pump P2. The first circulation pump P1 moves the fluid in the first outflow channel unit 41 and the second circulation pump P2 moves the fluid in the second outflow channel unit 42. [

Here, the circulation pump may be a peristaltic pump that provides a suction force in both directions, so that fluid can pass between the sub-pipe 12 and the extraction container 25. In other words, the fluid can be introduced into or discharged from the inlet pipe member 21 and the inlet and outlet channel portions, respectively.

The control unit C controls the opening / closing valve 14, the rotation driving unit M, the inlet / outlet valve, and the circulation pump by interlocking them. The control unit C replaces the operation description of the nucleated cell extracting apparatus described later.

The apparatus for extracting a nucleated cell according to an embodiment of the present invention may further include an active light unit (L).

The active light portion L irradiates light to at least one of the sub-pipe 12, the extraction pack G3, and the outflow channel portion. Here, the sub-pipe 12 to which the light is irradiated is a sub-pipe 12 to which the extraction pack G3 is connected, and the outflow channel portion to which the light is irradiated is connected to the first outflow channel portion 41 )to be.

The active light portion L is irradiated with light having a wavelength corresponding to red light or near-infrared light. In more detail, the wavelength of the light irradiated from the active light portion L may be 630 nm to 700 nm. When the wavelength of light is within the set range (630 nm to 700 nm), the nucleated cells can be activated by centrifugation. However, when the wavelength of light deviates from the set range (630 nm to 700 nm), light does not participate in the activation of nucleated cells. Particularly, the activation light L can emit light having a wavelength of 635 nm, thereby maximizing the activation of nucleated cells.

Hereinafter, the operation of the apparatus for extracting nucleated cells according to an embodiment of the present invention will be described.

The integral valve unit 10 and the extraction unit 20 are coupled to the main body 30 and the flow paths are interconnected in a state where both the on-off valve 14 and the on-off valve are closed.

First, while the first circulation pump P1 is operated in the normal direction, the first valve V21 is opened and the valve V11 is opened. Then, the tissue extract is supplied to the accommodating portion 261 through the main piping 11 and the first inlet / outlet flow path portion 41. [

At this time, when the tissue extract of the stock solution pack G1 is exhausted, the stock solution valve V11 is closed.

Second, the cleaning valve V14 is opened in a state in which the first circulation pump P1 and the first access valve V21 are opened. The cleaning liquid is finally supplied to the accommodating portion 261 through the main pipe 11 and the first inlet and outlet flow path portion 41 so that the main pipe 11 and the first inlet and outlet flow path portion 41 are connected to each other, Can be cleaned.

Third, the cleaning valve V14 is closed while the first circulation pump P1 is stopped, and then the extraction container 25 is rotated by the operation of the rotation driving part M, whereby the tissue extract and the cleaning solution are set in advance Lt; / RTI >

At this time, the extraction container 25 may be rotated in the forward and reverse directions by the rotation driving unit M, and may be repeatedly rotated and stopped by the rotation driving unit M.

Fourth, when the extraction container 25 is stopped for a predetermined time, a cleaning liquid, blood, body fluids and the like are stacked from the bottom in the container 261 in the extraction container 25, And an oil component lighter than the structure is laminated on the upper side thereof. Here, the cleaning solution, blood, body fluid, etc. disposed on the lower side of the cleaned tissue are regarded as foreign matter separated from the tissue extract.

Fifth, while the first circulation pump P1 is operated in the reverse direction, the first inlet valve V21 is opened and the waste liquid valve V15 is opened. Then, the foreign matters stacked on the bottom of the accommodating portion 261 are moved to the waste liquid pack G5.

After the foreign object is transferred to the waste liquid pack G5, the waste liquid valve V15 is closed and the first circulation pump P1 is operated in the forward direction to open the cleaning valve V14, 261, and then the third, fourth, and fifth processes are repeatedly repeated a predetermined number of times to remove all the foreign materials stacked on the bottom of the accommodating portion 261. When all the foreign matter is moved to the waste liquid pack G5, the waste liquid valve V15 is closed.

Sixth, the first circulation pump P1 is operated in the forward direction to open the enzyme valve V12. Then, the degrading enzyme is supplied to the accommodating portion 261 via the main pipe 11 and the first inlet / outlet flow path portion 41. [

At this time, when the enzyme of the enzyme pack (G2) is exhausted, the enzyme valve (V12) is closed.

Here, when the second process is added, it is possible to prevent the decomposition enzyme from remaining in the main pipeline 11 and the first inlet / outlet flow path portion 41 by the washing liquid.

Seventh, the enzyme valve V12 and the cleaning valve V14 are closed while the first circulation pump P1 is stopped, and then the extraction vessel 25 is rotated by the operation of the rotation driving unit M, The tissue remaining in the receiving portion 261 and the degrading enzyme are agitated for a predetermined period of time.

The extraction vessel 25 is maintained at a temperature of 36 to 38 ° C and substantially 37 ° C using a separate heating medium at the time of stirring, thereby improving the decomposition rate of the tissue and the degradation rate of the tissue by the decomposition enzyme.

At this time, the extraction container 25 may be rotated in the forward and reverse directions by the rotation driving unit M, and may be repeatedly rotated and stopped by the rotation driving unit M.

When the extraction container 25 is stopped for a preset time, an aqueous solution containing nucleated cells decomposed from the tissue from the bottom is stacked in the receiving part 261 of the extraction container 25, and nucleated cells Which is not decomposed to the upper side of the aqueous solution, is laminated, and a light oil component is laminated on the upper side of the non-decomposed tissue. Here, the undegraded tissue and the oil component, which are laminated on the upper side of the aqueous solution containing the nucleated cells, are regarded as foreign substances separated from the tissue extract.

At this time, the aqueous solution containing the nucleated cells may be separately extracted through the reverse operation of the first circulation pump P1 and the opening / closing operation of the first inlet / outlet valve V21, or may be stored in a separate solution pack.

Eighth, when the stirring of the tissue and the degrading enzyme is completed, the rotation driving unit M rotates the extraction container 25 at a high speed. Then, after the seventh step, the foreign matter is raised upward to the receiving portion 261 and is collected by the collecting member 23 and guided to the second exit pipe 213. In the aqueous solution containing the nucleated cells, the relatively heavier nucleated cells are accommodated in the nucleated cell separation groove 262 by the centrifugal force of the extraction container 25.

At this time, as the second inlet / outlet valve V22 and the waste liquid valve V15 are intermittently opened and closed, the foreign matter introduced into the second outlet pipe 213 is transferred to the waste liquid pack G5. Here, since the second circulation pump P2 is operated in the reverse direction, the movement of the foreign matter can be accelerated.

The main pipe (11) and the outflow channel portion are connected to each other by intermittently opening and closing the cleaning valve (V14) and operating either the first circulation pump (P1) or the second circulation pump (P2) It can be cleaned and kept clean. At this time, the washing liquid flowing into the accommodating portion 261 through the first inlet / outlet flow path portion 41 rises and can finally wash out the nucleated cells to be finally extracted through the nucleation grooves 262.

When the eighth process is completed, the nucleated cells 262 are mixed with a small amount of washing solution.

Ninth, the rotation driving unit M is stopped and the first and second valves V21 and V13 are opened while the first circulation pump P1 is operated in the reverse direction.

More specifically, when the rotation driving unit M is stopped, the nucleated cells mixed with the nucleation groove 262 and the washing solution are moved to the bottom of the accommodating part 261. When the first inlet valve V21 and the extraction valve V13 are opened while the first circulation pump P1 is operated in the opposite direction, (G3).

At this time, the cleaning liquid continuously cleans the main pipe 11 and the first inlet / outlet flow path portion 41 through the eighth process, and thus the nuclide cells are prevented from being contaminated during movement.

A sub-pipe 12 connected to the first outflow channel unit 41 and the extraction pack G3 via the active light unit L when the nucleated cells are moved to the extraction pack G3, G3) can be activated to lighten the tired nucleated cells.

According to the integrated valve unit and the nucleated cell extracting apparatus using the integrated valve unit, the nucleated cells can be extracted from the tissue extract by centrifuging the tissue extract. Particularly, through the integral valve unit 10, it is possible to simplify the movement path of the valve and the fluid, reduce the moving distance of the fluid, and prevent deformation of the tissue extract.

The main piping 11 and the sub piping 12 are stably fixed through the integrated valve unit 10 to improve the opening and closing precision of the sub piping 12, It is possible to prevent the sub-pipe 12 from being damaged according to the operation. It is also possible to keep the main piping 11 branched from the plurality of sub piping 12 through the integrated valve unit 10 clean and to supply the fluid to the extraction unit 20 It is possible to prevent foreign matter from mixing with the fluid.

In addition, the tissue extract can be centrifugally separated by one extracting unit 20 through the detailed structure of the nucleated cell extracting apparatus, and the automation can be realized while maintaining airtightness from the tissue extract to the extraction of nucleated cells throughout the process. In addition, it is possible to activate the nucleated cells, which have been tired by centrifugation through the active light portion L, and to increase the regeneration efficiency of the nucleated cells. Also, the activation of the nucleated cells through the activated light (L) can be promoted, and the nucleated cells extracted by the nucleated cell extracting apparatus can be administered immediately. In addition, the extraction efficiency of the nucleated cells can be increased while performing both the washing and the decomposition and the extraction of the tissue extract through one extraction unit 20. In addition, through the detailed structure of the extraction unit 20, it is possible to concentrate the collection of the nucleated cells in the extraction container 25 and suppress or prevent the mixing of the nucleated cells and the foreign substances.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, Modify or modify the Software.

10: Integrated valve unit 11: Main piping 12: Sub piping
13: fixed block 14: opening / closing valve 14a:
14b: piston part 15: fixed body V11: raw fluid valve
V12: Enzyme valve V13: Extraction valve V14: Cleaning valve
V15: Waste liquid valve G1: Solution pack G2: Enzyme pack
G3: Extraction Pack G4: Washing Pack G5: Waste Pack
20: extraction unit 21: entry / exit pipe member 211: first inlet /
212: first flow path 213: second outflow tube 214: second flow path
215: intermediate member 22: rotation support member 23: collecting member
231 collecting part 232 guiding part 233 through hole
25: extraction container 26: extraction body 261:
262: nucleus separation groove 263: bottom inclined portion 264: collecting hole
265: discharge inclined portion 28: extraction plug 281:
30: main body 41: first outflow channel portion 42: second outflow channel portion
M: rotation drive part C: control part L:
V21: first inlet valve V22: second inlet valve P1: first circulation valve
P2: second circulation valve

Claims (9)

Hollow main piping;
An enzyme pack for storing a degrading enzyme for decomposing tissue in a tissue extract, an extraction pack for storing nucleated cells separated from the tissue, and a washing solution for mixing the tissue extract or nucleated cells And a plurality of sub-pipelines for connecting a waste solution pack for storing foreign matter separated from the tissue extract to the main piping, respectively;
A fixed block in which the main pipe and the sub pipe are inserted and supported; And
And a plurality of open / close valves individually opening and closing the plurality of sub-pipes supported by the fixed block,
Wherein the fixed block has a cylindrical shape,
Wherein one of the main pipe and the sub-pipe is disposed in the longitudinal direction of the fixed block, the other of the main pipe and the sub-pipe surrounds the fixed block,
Wherein the sub-pipe is disposed at one side edge of the main pipe among the plurality of sub-pipes, the waste liquid pack is connected to the sub-
Wherein the cleaning pack is connected to the sub-pipe located on the side of the sub-pipe to which the waste liquid pack is connected. The method according to claim 1,
And a fixed body detachably fixing the fixed block,
The on-
A cylinder coupled to the stationary body; And
And a piston portion reciprocally coupled to the cylinder portion to press the sub-pipe. delete delete An integrated valve unit according to claim 1 or 2;
An extraction unit having a first inlet pipe connected to the other end of the main pipe for extracting the nucleated cells from the tissue extract and a second inlet pipe connected to one end of the main pipe;
A main body to which the integral valve unit and the extraction unit are coupled;
A rotation driving unit for rotating the extraction unit;
A first inlet / outlet flow path for connecting the other end of the main pipe to the first inlet / outlet pipe; and a second inlet / outlet flow path for connecting one end of the main pipe to the second outlet / outlet pipe;
A first inlet / outlet valve for opening / closing the first inlet / outlet flow passage, and a second inlet / outlet valve for opening / closing the second entrance / exit flow passage;
A circulation pump for moving the fluid in the outflow channel portion; And
And a control unit for controlling the opening / closing valve, the rotation driving unit, the inlet / outlet valve, and the circulation pump in cooperation with each other. 6. The method of claim 5,
Further comprising an active light part for irradiating light to at least one of the sub-pipe, the extraction pack, and the outflow channel part. The method according to claim 6,
Wherein the active light portion is irradiated with light having a wavelength corresponding to red light or near-infrared light. 6. The method of claim 5,
Wherein the extraction unit comprises:
A first inlet / outlet pipe connected to the main pipe through the inlet / outlet flow path in a state detachably coupled to the main body, wherein a first flow path is formed, and a second flow path surrounding the first inlet / An entrance pipe member including a second access pipe;
A collection part fixed to the first outlet pipe so as to face the second outlet pipe and having a concave surface facing the second outlet pipe; and a second outlet pipe extending from the edge of the collecting part toward the first outlet pipe A collecting member including an inducing portion protruding from a collecting portion; And
A plurality of nuclide separation grooves formed on the outer periphery of the receptacle, the receptacle being rotated by the rotation driving unit in a state where the entrance and exit tube members are rotatably inserted, A bottom inclined portion inclining downward from the bottom edge of the accommodating portion toward the bottom center of the accommodating portion; a collecting groove formed in the bottom center of the accommodating portion so as to insert the end portion of the first access pipe; And an extracting container having a discharge inclining portion inclined upward toward an edge of the collecting portion. 9. The method of claim 8,
Wherein the nucleated cell separation groove is formed in a cone shape whose diameter decreases from the entrance to the inside.

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