KR101699857B1 - Apparatus and System for Optical Imaging using Near Infrared Fluorescence and Method for controlling the same - Google Patents

Abstract

The present invention relates to a medical optical imaging apparatus, and more particularly, to a medical optical imaging apparatus using a near-infrared ray nano-fluorescent substance to observe microscopic structures inside a living tissue, and to perform visual inspection of position information in real- A first irradiation module for irradiating near infrared rays having a first wavelength band and a second irradiation module for irradiating near infrared rays having a second wavelength band, A probe module unit connected to the main body module unit via a connector and having an intravital and an endoscope probe interposed between the zoom lens and the alternative lens, Fluorescence image of a specific region through a real-time color image and a near-infrared fluorescent substance, To combine outputs.

Description

Technical Field [0001] The present invention relates to an optical imaging apparatus and system using near-infrared fluorescence, and a control method thereof.

The present invention relates to a medical optical imaging apparatus, and more particularly, to an optical imaging apparatus using near-infrared nano-fluorescent material for observing the microstructure of a living body tissue for observation, A video apparatus and a system, and a control method thereof.

An image guided surgery system is generally used to assist a surgeon in locating a surgical instrument during an operation, and may further be used for pre-surgery simulation or surgical planning.

In the case of neuro-surgery such as surgery, especially brain surgery, it is very difficult or even impossible for the surgeon to move the surgical tool while looking directly at the patient's surgical site.

In general, the image guided surgery system displays CT (computed tomography) images or MRI (magnetic resonance) images taken before surgery on a display device such as a monitor, and positions and positions surgical tools corresponding to the images.

Therefore, the surgeon can grasp the relative position of the operation part and the surgical tool through the display device of the image guided surgery system, and by using the surgical guiding device, .

However, since the scale and direction of the image are different from what the surgeon actually sees during the operation using the image guided surgery system, it is difficult for the surgeon to accurately grasp the position of the surgical site in the operation requiring precision like brain surgery Because of this, the position of the surgical instruments such as the scalpel was changed many times, and the operation time was delayed and the probability of side effects was high.

In order to compensate for the disadvantages of such an image guided surgical system, most of the prior art optical imaging system using fluorescence has been used for ultraviolet light and a fluorescent material, which are used for the absorption loss of fluorescence, scattering by body tissue, It is used for the analysis of the unit cell and the characteristics of biomaterial, and it is used for partial image confirmation for image guided surgery.

Nevertheless, the information of most imaging systems is focused on the diagnosis, and since the confirmation of the microscopic region in real time at the time of operation is unclear, image guiding surgery is proceeding depending on the doctor 's experience.

In particular, most of these imaging techniques have limitations such as the absence of tissue-specific fluorescent molecules and the limitation of real-time imaging. At present, only partial image-guided surgery is performed through general optical imaging techniques.

Therefore, in the case of surgery for a specific disease (eg, pancreatic cancer) that depends only on the doctor's experience, diagnosis can be diagnosed and treated only through a tissue-specific contrast agent, and the patient's physiological changes and changes in micro- The need for the development of a real - time image guided surgery system has prompted many studies centering on the United States and other developed countries.

However, the present research and development has limitations such as the absence of tissue-specific fluorescent molecules and the limitation of high-efficiency real-time fluorescence image acquisition system.

Korean Patent Publication No. 10-2013-0118086 Korean Patent Publication No. 10-2015-0019311

In order to solve the problem of the medical optical imaging apparatus of the related art, the present invention provides an apparatus and a method for observing microstructure inside biological tissue using near-infrared nano fluorescent material, An object of the present invention is to provide an optical imaging apparatus and system using near-infrared fluorescence which can be visually confirmed, and a control method thereof.

It is an object of the present invention to provide a clinical real-time optical imaging system for diagnosis and treatment of diseases, which can solve the limitations of the absence of tissue-specific fluorescent molecules and the limitation of high-efficiency real-time fluorescence image acquisition system.

The objective of the present invention is to provide a tracking image system for cancer and endocrine diseases through the development of a biocompatible near infrared ray nano-phosphor having positive ion properties and the development of a near-infrared optical component of high-resolution optical imaging equipment capable of accurately detecting the same. .

The present invention relates to a method for detecting intra-body images of micro-parts by combining intravital and endoscope probes at the bottom, using near-infrared wavelengths and observing microstructures in living tissues using near-infrared nano- The present invention also provides an optical imaging apparatus and system using the near-infrared fluorescence, and a control method thereof.

The objects of the present invention are not limited to the above-mentioned objects, and other objects not mentioned can be clearly understood by those skilled in the art from the following description.

To achieve the above object, an optical imaging apparatus using near-infrared fluorescence according to the present invention includes a first irradiation module for irradiating near-infrared rays having a first wavelength band, a second irradiation module for irradiating near- A probe module connected to the main body module through a connector and having an intravital and an endoscope probe interposed between the zoom lens and the alternative lens, And combines a real-time color image with a fluorescence image of a specific region through the near-infrared fluorescence optical body and outputs the fluorescence image.

Here, the main body module unit includes first and second BPFs positioned on the optical path of the first and second irradiation modules and performing band filtering, and first and second BPFs disposed on the optical path of the first and second irradiation modules, A tube constituting a path of light selectively passed through the dichroic filter, a motorized zoom lens positioned at one end of the tube and controlling the magnification by driving the motor, And a motorized focusing lens coupled to the motorized zoom lens to perform focusing control.

And the first and second irradiation modules form near-infrared fluorescence channels of different wavelength bands in the near-infrared region of 650 to 900 nm.

The first irradiation module constitutes a near-infrared fluorescence channel having a color channel and a wavelength of 760 nm, and the second irradiation module forms a near-infrared fluorescence channel having a wavelength of 660 nm.

The first and second irradiation modules are characterized by alternately configuring a near-infrared fluorescence channel.

In order to obtain fluorescence images, a near-infrared nano-nano-nano-nano-nano-nano-nano-nano-nano-nano- And a phosphor is used.

According to another aspect of the present invention, there is provided an optical imaging system using near-infrared fluorescence, comprising: a color channel control unit configured to control a first irradiating unit for irradiating a near-infrared ray having a first wavelength band and a first near- A second near-infrared fluorescence channel control unit for controlling a second irradiation unit for irradiating a near-infrared ray having a second wavelength band to form a second near-infrared fluorescence channel; An emission photon measurement unit for measuring photons emitted from the irradiation region under the control of the control unit, a color image by the color channel control unit, and an image for acquiring the first and second near-infrared fluorescence images by the first and second near- An image obtaining unit for obtaining a color image by combining the color image obtained by the image obtaining unit with a 1,2- An image combining unit, and an image providing unit that outputs an optical image using near-infrared fluorescence combined in the image combining unit in real time.

Here, the first and second irradiation units form near-infrared fluorescence channels of different wavelength bands in the near-infrared region of 650 to 900 nm.

The first irradiation unit forms a near-infrared fluorescence channel having a color channel and a wavelength of 760 nm, and the second irradiation unit forms a near-infrared fluorescence channel having a wavelength of 660 nm.

The first and second near-infrared fluorescence channel control units alternately configure the first and second near-infrared fluorescence channels.

The first and second irradiation units are each provided with a CCD module as an imaging device for image acquisition.

According to another aspect of the present invention, there is provided a method of controlling an optical imaging apparatus using near-infrared fluorescence, the method comprising: controlling a color channel and first and second near-infrared fluorescence channels when the nanofluorescent material is injected into a body- The method comprising the steps of: constructing first and second near-infrared fluorescence channels alternately under the control of first and second near-infrared fluorescence channel control units, performing photon measurement according to the first and second near-infrared fluorescence channel configurations, Combining the first and second near-infrared fluorescence images according to the emitted photon measurement in real time with the color image, outputting the optical image using near-infrared fluorescence combined with the color image and the 1,2 near-infrared fluorescence image, ; And

Here, in the step of alternately constructing the first and second near-infrared fluorescence channels, near infrared rays are alternately irradiated in the first and second irradiation units for irradiating the near-infrared rays to form the first and second near- And the first and second near-infrared fluorescence channels are alternately formed by a dichroic filter that selectively passes light according to wavelengths.

The optical imaging apparatus and system using the near-infrared fluorescence according to the present invention and the control method thereof have the following effects.

First, it is possible to accurately judge a treatment site by combining a real-time color image and a fluorescence image of a specific region through a near-infrared fluorescence body.

Secondly, multi-channel type fluorescence can be simultaneously acquired in real time so that only a variety of diseased tissues can be detected and only a desired biotissue can be visualized.

Third, Intravital and Endoscope probes can be attached to the lower part to enable visualization of the inside of the microscopic area, and simultaneous observation of diagnosis and treatment is possible.

Fourth, it is possible to enhance the detection ability of diseased tissues through the near-infrared nanoporous phosphor, and to induce and diagnosis / treatment for a long time.

Fifth, since photons emitted from different wavelengths of near infrared rays can be combined with color images in real time, it can be applied to various application fields because it is possible to accurately determine diagnosis and treatment sites.

Sixth, observation of the tissue at the cellular level enables precise diagnosis of disease malignancy and metastasis, and provides more pathological information such as a clear boundary between normal tissue and diseased tissue, thereby enabling more accurate diagnosis and treatment of disease.

Seventh, the optical imaging system can be constructed at a low cost, the cell level can be detected with high spatial resolution, and the non-radiation system is excellent in biostability.

1 is a block diagram showing the characteristics of an optical imaging apparatus using near-infrared fluorescence according to the present invention
FIGS. 2A and 2B are a structural diagram and a wavelength characteristic graph of an optical imaging apparatus using near-infrared fluorescence according to the present invention
FIGS. 3 and 4 show a detailed configuration of an optical imaging apparatus using near-infrared fluorescence according to the present invention
5 is a longitudinal sectional view of an optical imaging apparatus using near-infrared fluorescence according to the present invention
6 is a schematic diagram of an optical imaging system using near-infrared fluorescence according to the present invention
7 is a flowchart for controlling an optical imaging system using near-infrared fluorescence according to the present invention.
8A is an image showing the diagnosis of breast cancer through an optical imaging system using near-infrared fluorescence according to the present invention
8B is an image showing pancreatic cancer targeting and diagnosis through an optical imaging system using near-infrared fluorescence according to the present invention

Hereinafter, preferred embodiments of an optical imaging apparatus and system using near-infrared fluorescence according to the present invention and a control method thereof will be described in detail as follows.

The features and advantages of the optical imaging apparatus and system using near-infrared fluorescence according to the present invention and the control method thereof will be apparent from the following detailed description of each embodiment.

FIG. 1 is a configuration diagram showing the characteristics of an optical imaging apparatus using near-infrared fluorescence according to the present invention, and FIGS. 2 (a) and 2 (b) are graphs and wavelength characteristics of an optical imaging apparatus using near-infrared fluorescence according to the present invention.

The present invention relates to the development of a clinical real-time optical imaging system for diagnosis and treatment of diseases, and to a system for optical image guided surgery based on biocompatible near infrared nano-phosphors.

The optical imaging device and system using the near-infrared fluorescence according to the present invention and the control method thereof can be used for the inspection and the treatment by observing the microstructure inside the living tissue using the near infrared ray wavelength and the near infrared ray nano- Real-time position information can be visually confirmed.

In addition, Intravital and Endoscope probes can be attached to the lower part to enable image confirmation in the body of a microscopic region, and simultaneous observation of diagnosis and treatment is possible.

As shown in FIG. 1, unlike the prior art, it is possible to accurately determine a treatment site by combining a real-time color image, near-infrared fluorescence, and fluorescence image of a specific region of a sieve, So that images of fluorescence of a multi-channel type can be acquired simultaneously in real time.

The present invention relates to a wide field of view (> 10 cm) and a long working distance (> 25 m) for securing the surgical area as shown in FIGS. 2A and 2B for developing optical image optical components and systems for real- cm < / RTI >), while at the same time configuring the optical system to enable a resolution of 10 LP or higher for observing the tissue level of the tissue.

In addition, through the use of one color channel and two near infrared fluorescence channels (700nm / 800nm), clear images of the normal tissues and diseased tissues are obtained. For this purpose, high sensitivity optical filtering technology, optical component technology and reverse engineering of biological systems reverse engineering) model to realize a 3D organization structure imaging system using 2D fluorescence image.

Here, the wavelength band (700 nm / 800 nm) for forming the two near-infrared fluorescence channels is described as an example, but it is not limited thereto.

In one embodiment of the present invention, the wavelength band for forming two near-infrared fluorescence channels is 760 nm / 660 nm.

The detailed configuration of an optical imaging apparatus using near-infrared fluorescence according to the present invention is as follows.

FIG. 3 and FIG. 4 are detailed views of an optical imaging apparatus using near-infrared fluorescence according to the present invention, and FIG. 5 is a longitudinal sectional view of an optical imaging apparatus using near-infrared fluorescence according to the present invention.

The optical imaging apparatus using near-infrared fluorescence according to the present invention comprises a body module unit and a probe module unit coupled to a lower portion of the body module unit.

The body module unit includes a first irradiation module 41 for irradiating near infrared rays having a first wavelength band, a second irradiation module 42 for irradiating near infrared rays having a second wavelength band, a first irradiation module 41, First and second BPFs 43 and 44 located in the optical path of the first and second BPFs 43 and 44 to perform band filtering and first and second irradiation modules 41 and 42 A dichroic filter 45 for selectively passing the light according to the wavelength, and a light path selectively passed through the dichroic filter 45. [ A motorized zoom lens 47 which is located at one end of the tube 46 to control magnification by motor driving and a motorized focusing lens which is combined with the motorized zoom lens 47 and performs focusing control 48).

The probe module unit is connected to the output terminal of the main body module unit through a connector 49 and is connected to an intravital and an endoscope probe 52 via a zoom lens 50 and an alternative lens 51 Structure.

The configuration of the main body module unit and the probe module unit and the lens arrangement are shown as an example and are not limited to the shapes and structures as shown in Figs.

Here, the first and second irradiation modules 41 and 42 constitute near-infrared fluorescence channels of different wavelength bands in the near-infrared region of 650 to 900 nm.

Preferably, the first irradiation module 41 constitutes a color channel and a near infrared ray fluorescence channel with a wavelength of 760 nm, and the second irradiation module 42 can constitute a near infrared ray fluorescence channel with a wavelength of 660 nm, but is not limited thereto.

The first and second irradiation modules 41 and 42 include a CCD module as an imaging device for image acquisition.

In the present invention, a biocompatible near infrared ray nano fluorescent substance for fluorescence image acquisition is used and a biocompatible near infrared ray nano fluorescent substance is prepared through synthesis of several derivatives of polymethine indocyanine core.

It is also designed to enable target detection through chemical bonding with various ligands, and the surface net charge of the final form compound bound to the ligand is zero.

Designed nanophosphor allows 90% or more of excretion through kidney within 4 hours of intravenous injection, minimizing non-specific signal in general tissues and enabling high signal contrast to target tissues.

In other words, such a nanophosphor is optimized for the pathway of circulation and excretion in the living body. After the intravenous injection, the equilibrium state in the inside / outside of the vein is efficiently formed and moves quickly to the target tissue, and the residue is absorbed But is renal filtration through the kidneys.

Fluorescent molecules undergo cell membrane binding and endocytosis at the molecular level, which allows for high specificity, sensitivity, and selectivity of the molecule itself I make it.

The present invention makes it possible to apply microscopic images such as cancer tissue detection and application to diagnosis of pancreatic cancer by using such a nanophosphor.

Previous fluorescers (eg, methylene blue) allow high-resolution, real-time imaging, multi-channel imaging even small cancer cells that can not be detected.

Since optical images in the near infrared region (650-900 nm) are excellent in optical characteristics (high light transmittance, low absorbance and scattering degree, low autofluorescence) for a living organism, . ≪ / RTI >

Therefore, it is possible to perform efficient image guiding surgery by combining with an optical system capable of obtaining a sufficient operation clock, to construct a low-cost optical imaging system, to enable cell level detection with high spatial resolution, Since it is a radiation system, it has an advantage of being excellent in biostability.

The system including the optical imaging apparatus using near-infrared fluorescence according to the present invention and the control method thereof will be described as follows.

6 is a configuration diagram of an optical imaging system using near-infrared fluorescence according to the present invention.

The optical imaging system using near-infrared fluorescence according to the present invention includes a color channel control unit 61 for controlling a first irradiating unit 64 for irradiating near-infrared rays having a first wavelength band to constitute a color channel, A first near infrared ray fluorescence channel control unit 62 that controls the first irradiation unit 64 for irradiating near infrared rays to constitute a first near infrared ray fluorescent channel and a second irradiation unit for irradiating near infrared rays having a second wavelength band 65) to control the photon emitted from the irradiation region under the control of the second near-infrared fluorescence channel control unit 63 and the first and second near-infrared fluorescence channel control units 62, 63 constituting the second near- An emission photon measurement unit 66 for measuring the fluorescence intensity of the first and second near infrared ray fluorescence channels 62 and 63 and a color image obtained by the color channel control unit 61 and first and second near-An image combining unit 68 that combines the color image obtained by the image obtaining unit 67 and the 1,2 Near Infrared fluorescence image, and an optical unit 65 that combines the near- And an image providing unit 69 for outputting an image.

Here, the first and second irradiation units 64 and 65 constitute near-infrared fluorescence channels of different wavelength bands in the near-infrared region of 650 to 900 nm.

Preferably, the first irradiating unit 64 constitutes a near-infrared fluorescence channel having a color channel and a wavelength of 760 nm, and the second irradiating unit 65 can constitute a near-infrared fluorescence channel having a wavelength of 660 nm, but is not limited thereto.

The first and second irradiation units 64 and 65 include a CCD module as an imaging device for image acquisition.

7 is a flowchart for controlling an optical imaging system using near-infrared fluorescence according to the present invention.

First, the nano-fluorescent substance is injected into a body-specific region (S701), and the color channel and the first and second near-infrared fluorescence channels 62 and 63 are controlled by the color channel control unit 61 and the first and second near- (S702). ≪ RTI ID = 0.0 >

Here, the nanophosphor is prepared through the synthesis of various derivatives of polymethine indocyanine core, enables target detection through chemical bonding with various ligands, and is capable of detecting the surface of the final compound bound to the ligand It is designed so that the surface net charge becomes zero.

Subsequently, the first and second near-infrared fluorescent channels are alternately configured by the control of the first and second near-infrared fluorescent channel controllers 62 and 63. (S703)

Here, in the process of alternately constructing the first and second near-infrared fluorescence channels, the near-infrared irradiation in the first and second irradiation units 64 and 65 may be alternately performed, or alternatively, And a dichroic filter passing through the filter.

The photon measurement is performed according to the first and second near-infrared fluorescence channel configurations (S704)

Then, a color image captured in real time is acquired (S705), fluorescence images according to the emitted photon measurement are combined with the color image in real time (S706), and the color image and near-infrared fluorescence combined with the near- And outputs an image (S707)

Likewise, the first and second near-infrared fluorescence channels constitute near-infrared fluorescence channels of different wavelength bands in the 650 to 900 nm near infrared region.

Preferably a near infrared ray fluorescent channel with a wavelength of 760 nm, and can constitute a near infrared ray fluorescent channel with a wavelength of 660 nm, but is not limited thereto.

8A is an image showing the diagnosis of breast cancer through an optical imaging system using near-infrared fluorescence according to the present invention.

And FIG. 8B is an image showing pancreatic cancer targeting and diagnosis through an optical imaging system using near-infrared fluorescence according to the present invention.

The optical imaging apparatus and system using the near-infrared fluorescence according to the present invention and the control method thereof can improve the detection ability of diseased tissues through the biocompatible near infrared ray nanoporous phosphor and visualize only the desired biotissue, So that it can be applied to a long-time image guided surgery.

Since optical images in the infrared region (650 to 900 nm) are excellent in optical characteristics (high light transmittance, low absorbance and scattering, low autofluorescence) for living organisms, You can lead.

In view of the above characteristics, the present invention combines the intravital and endoscope probes at the bottom to enable visualization of images in the body of the fine region. Using the near-infrared wavelengths and using the near-infrared nano- It is possible to visually check the real-time location information in the examination and treatment through observing the microstructure in the tissue and the surgical operation of the specific site.

As described above, it will be understood that the present invention is implemented in a modified form without departing from the essential characteristics of the present invention.

It is therefore to be understood that the specified embodiments are to be considered in an illustrative rather than a restrictive sense and that the scope of the invention is indicated by the appended claims rather than by the foregoing description and that all such differences falling within the scope of equivalents thereof are intended to be embraced therein It should be interpreted.

61. Color channel control unit 62. First near-infrared fluorescence channel control unit
63. Second near-infrared fluorescence channel control unit 64. First irradiation unit
65. Second irradiation section 66. Emission photon measurement section
67. Image acquiring unit 68. Image combining unit
69. Video assistant

Claims (13)

A color channel control unit having a first irradiation module for irradiating near infrared rays having a first wavelength band and a second irradiation module for irradiating near infrared rays having a second wavelength band and controlling the first irradiation module to constitute a color channel; A second near-infrared fluorescence channel control unit constituting a first near-infrared fluorescence channel, a second near-infrared fluorescence channel control unit controlling a second irradiation module to constitute a second near-infrared fluorescence channel, and an emission photon An image acquiring unit that acquires in real time the first and second near-infrared fluorescence images by the color image and the first and second near-infrared fluorescence channel controllers by the color channel controller, A main body module unit including an image combining unit for combining two near-infrared fluorescence images, combining the acquired images, and outputting the combined images;
And a probe module unit connected to the main body module unit through a connector and having an intravital and an endoscope probe interposed between the zoom lens and the alternative lens,
Real-time color image and a fluorescence image of a specific region through a near-infrared fluorescence optical body, and outputs the fluorescence image. The apparatus according to claim 1,
A first and a second BPFs located in the optical path of the first and second irradiation modules and performing band filtering,
A dichroic filter positioned in the optical path of the first and second irradiation modules through the first and second BPFs to selectively pass light according to wavelengths;
A tube constituting a path of light selectively passed through the dichroic filter,
A motorized zoom lens positioned at one end of the tube to control magnification by motor driving,
And a motorized focusing lens coupled to the motorized zoom lens to perform focusing control. The optical imaging apparatus using near-infrared fluorescence according to claim 1, wherein the first and second irradiation modules constitute near-infrared fluorescence channels of different wavelength bands in the near-infrared region of 650 to 900 nm. The method according to claim 1, wherein the first irradiation module comprises a color channel and a near infrared ray fluorescence channel having a wavelength of 760 nm,
And the second irradiation module constitutes a near-infrared fluorescence channel having a wavelength of 660 nm. The optical imaging apparatus according to claim 1, wherein the first and second irradiation modules alternately configure a near-infrared fluorescence channel. The method of claim 1, wherein, for fluorescence image acquisition,
It is produced through the synthesis of derivatives of polymethine indocyanine core,
Wherein the near ultraviolet nano fluorescent substance is designed so that the surface net charge of the compound of the final form bound to the ligand is zero. A first near-infrared fluorescence channel controller configured to configure a color channel and a first near-infrared fluorescence channel by controlling a first irradiator for irradiating near-infrared rays having a first wavelength band;
A second near-infrared fluorescence channel control unit configured to control a second irradiation unit that irradiates a near-infrared ray having a second wavelength band to perform a second irradiation to constitute a second near-infrared fluorescence channel;
An emission photon measurement unit for measuring photons emitted from the irradiation region under the control of the first and second near-infrared fluorescence channel control units;
An image acquisition unit for acquiring, in real time, a color image by the color channel control unit and first and second near-infrared fluorescence images by the first and second near-infrared fluorescence channel control units;
An image combining unit for combining the color image obtained by the image acquiring unit and the 1,2 Near Infrared fluorescence image;
And an image providing unit for outputting an optical image using near-infrared fluorescence combined in the image combining unit in real time. 8. The apparatus according to claim 7,
Wherein the near-infrared fluorescence channel of different wavelength bands is constituted in the near-infrared region of 650 to 900 nm. 8. The apparatus according to claim 7, wherein the first irradiating unit constitutes a color channel and a near-infrared fluorescence channel having a wavelength of 760 nm,
And the second irradiation unit forms a near-infrared fluorescence channel having a wavelength of 660 nm. 8. The apparatus of claim 7, wherein the first and second near-
Wherein the first and second near-infrared fluorescence channels are alternately arranged. 8. The optical imaging system according to claim 7, wherein the first and second irradiation units are provided with a CCD module as an imaging device for image acquisition. Controlling the color channel and the first and second near-infrared fluorescence channels when the nanofluorescent material is injected into the body-specific region in the color channel control unit and the first and second near-infrared fluorescence channel control units;
Alternately constructing the first and second near-infrared fluorescence channels under the control of the first and second near-infrared fluorescence channel control units;
Measuring photons emitted from the emitted photon measuring unit according to the first and second near-infrared fluorescence channel configurations;
Acquiring a color image captured in real time by the image acquiring unit, and combining the first and second near-infrared fluorescence images according to the photon measurement emitted from the image combining unit to the color image in real time;
A method of controlling an optical imaging system using near-infrared fluorescence, comprising: outputting an optical image using near-infrared fluorescence combined with a color image and a 1,2 near-infrared fluorescence image in an image providing unit. 13. The method of claim 12, wherein in the step of alternately constructing the first and second near-
In order to construct the first and second near-infrared fluorescence channels, the near-infrared irradiation in the first and second irradiation units for irradiating near-
Wherein the first and second near-infrared fluorescence channels are alternately arranged by a dichroic filter which is located in a light propagation path and selectively passes light according to a wavelength, / RTI >

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