KR101697863B1 - Oil-water separator for production of organic acid through organic acid fermentation by microorganism having improved oil-water separation efficiency by having specific three dimensional structure, and a device for production of organic acid including the same - Google Patents

Oil-water separator for production of organic acid through organic acid fermentation by microorganism having improved oil-water separation efficiency by having specific three dimensional structure, and a device for production of organic acid including the same Download PDF

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KR101697863B1
KR101697863B1 KR1020150060357A KR20150060357A KR101697863B1 KR 101697863 B1 KR101697863 B1 KR 101697863B1 KR 1020150060357 A KR1020150060357 A KR 1020150060357A KR 20150060357 A KR20150060357 A KR 20150060357A KR 101697863 B1 KR101697863 B1 KR 101697863B1
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organic acid
oil
fermentation
organic solvent
organic
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KR20160128653A (en
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이태호
상병인
전재성
전병승
박효정
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한양대학교 산학협력단
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/52—Propionic acid; Butyric acids

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Abstract

The present invention relates to an oil-water separator for an organic acid production apparatus by microbial organic acid fermentation and an apparatus for producing organic acid containing the same. More specifically, the present invention relates to a water- Wherein a central axis passing through the center of the transverse section of the one end and a center of the transverse section of the other end are disposed on different axes and a cross sectional area of the other end is larger than an area of the one cross section An oil-water separation vessel formed; A separation membrane of a porous hydrophobic material fixed to the transverse side of the one end of the transport space in a membrane form and having a plurality of water separation holes formed therein to allow the mixed solution to pass therethrough; An inlet port disposed at one end of the oil-water separation vessel for introducing the mixed fluid into the transportation space; An organic solvent discharge port disposed at the other end of the oil-water separation vessel for discharging an organic solvent separated from the fermentation broth and containing the extracted organic acid to the outside; And a fermentation liquid discharge port disposed at the other end of the oil-water separation vessel for discharging the fermentation broth separated from the organic solvent and extracted with the organic acid to the outside, and an oil water separator for organic acid production apparatus by microbial organic acid fermentation And an organic acid production apparatus.
According to the present invention, there is provided a water-oil separator for an organic acid production apparatus by microbial organic acid fermentation, which can easily separate organic acids extracted by an organic solvent from the fermentation broth without any additional solvent or additional process, Can be provided.

Description

TECHNICAL FIELD The present invention relates to an oil-water separator for an organic acid production apparatus by microbial organic acid fermentation having a three-dimensional shape for improving oil-water separation efficiency, and an apparatus for producing organic acid containing the organic acid fermentation by microorganism having improved oil- water separation efficiency by having specific three dimensional structure, and a device for production of organic acid including the same}

The present invention relates to an apparatus for producing an organic acid by microbial organic acid fermentation, comprising: an organic solvent containing an organic acid extracted by an organic solvent; and a water separator for separating the fermentation liquid from which the organic acid is extracted, To an organic acid production apparatus.

Conventional microbial fermentation reactions are used to produce various organic acid or alcohol-based compounds. For example, various organic acids such as acetic acid, propionic acid, butyric acid, and succinic acid can be produced through microbial fermentation reaction, and various alcohol compounds such as ethanol and butanol can also be produced through a microbial fermentation reaction. The resulting organic acids or alcohol compounds can be used as platform chemicals in a variety of chemical processes and can be used to synthesize materials with higher value through subsequent catalytic processes.

On the other hand, the term "organic acid fermentation by microorganisms" means a process for producing the above-mentioned various organic acids by incomplete oxidation of carbon compounds through fermentation reaction by microorganisms. FIG. 1 shows a conventional organic acid fermentation process. Referring to FIG. 1, a fermentation reaction by a microorganism is performed in an organic acid production fermenter 110, and a microorganism and a microorganism culture solution for performing organic acid fermentation are accommodated in the fermenter 110. In the organic acid producing fermenter 110, a mixture of a microorganism that performs organic acid fermentation and a fermentation product that is a result of the fermentation reaction by the microorganism is mixed. Therefore, in a subsequent extraction process, the mixture is transferred to the extractor 120, Is performed. Meanwhile, FIG. 1 shows an extraction method using an organic solvent in the separation of the produced organic acid from the culture medium of the microorganism, but other separation methods such as an electrodialysis method and a reverse osmosis membrane method may be used.

In the extraction method using an organic solvent, an extraction organic solvent for extracting only organic acid is introduced into the extractor 120 from the first extraction solvent reservoir 130, and the introduced organic solvent After the organic acid is extracted in the extractor 120, the separated organic solvent is separated into the fermentation liquid and the organic solvent from which the organic acid is extracted through the oil water separator 140, and the separated organic solvent is transferred to the second extraction solvent reservoir 150, The extraction organic solvent stored in the second extraction solvent reservoir 150 is an organic solvent containing an organic acid as a target material. Therefore, the organic acid is separated from the organic solvent through a separate catalytic process, and then the organic solvent separated organic acid is transferred to the first extraction solvent reservoir 130 again.

In the above-described series of organic acid fermentation processes, the process of separating the fermentation broth from which the organic acid has been extracted from the organic solvent containing organic acid by the water-oil separator 140 is important in terms of obtaining the produced organic acid at a higher yield In addition, there is a possibility that the organic solvent used for the extraction may exhibit toxicity to the microorganism, and furthermore, a more thorough separation is necessary in that the price of the organic solvent used is high.

Korean Patent Laid-Open Publication No. 10-2004-0091215 discloses a method for minimizing the amount of an organic solvent which is not recovered in an organic solvent used for extraction. More specifically, an organic solvent for extraction And other organic solvents such as tri-n-octylamine are mixed to facilitate the separation of the organic solvent used for extraction through a separate distillation process or the like.

However, the above-mentioned method complicates the entire process in that another organic solvent is required for further separation of the organic solvent or further additional processes are required, and the recovery rate of the organic solvent is also excellent It has a limitation.

Korean Patent Publication No. 10-2004-0091215

SUMMARY OF THE INVENTION Accordingly, the present invention has been made in view of the above problems, and it is an object of the present invention to provide a method for producing organic acids by microbial organic acid fermentation, which comprises extracting organic acids extracted by an organic solvent during a microbial fermentation process from a fermentation broth And a device for producing organic acid including the same, which can be easily separated with excellent efficiency, by using microorganism organic acid fermentation.

In order to solve the above problems,

And a transporting space for transporting the mixed liquid from one end to the other end, wherein the central axis passing through the center of the cross section of the one end and the center of the cross section of the other end are formed on different axis And an area of the transverse section at the other end is larger than an area of the transverse section at the one end;

A separation membrane of a porous hydrophobic material fixed to the transverse side of the one end of the transport space in a membrane form and having a plurality of water separation holes formed therein to allow the mixed solution to pass therethrough;

An inlet port disposed at one end of the oil-water separation vessel for introducing the mixed fluid into the transportation space;

An organic solvent discharge port disposed at the other end of the oil-water separation vessel for discharging an organic solvent separated from the fermentation broth and containing the extracted organic acid to the outside; And

And a fermentation liquid discharge port disposed at the other end of the oil-water separation vessel for separating the organic solvent from the organic solvent and discharging the fermentation liquid to the outside,

A water-oil separator for an organic acid production apparatus by microbial organic acid fermentation.

According to an embodiment of the present invention, the cross section of the other end may be two to nine times the area of the cross section of the one end.

According to another embodiment of the present invention, the volume of the other end may be 2 to 18 times the volume of the one end.

According to another embodiment of the present invention, there is provided a connection portion between the one end and the other end, the cross-sectional area of which increases from the one end toward the other end, and the volume of the connection portion is 1.5 to 2.5 times the volume of the one end. have.

According to another embodiment of the present invention, the separation membrane may be fixed within a range of one-third to one-half of the end of the one end, based on the direction of transport of the fermentation liquid inside the oil separation vessel.

According to another embodiment of the present invention, the oil-water separation vessel includes a binding groove recessed in the inner circumferential surface so that the rim of the separation membrane is inserted and fixed; And at least one of a rim of the one side of the separation membrane or a side of the other side of the separation membrane, which is disposed inside the binding groove, and which supports the separation membrane, .

According to another embodiment of the present invention, the thickness of the separation membrane is 10 mm to 300 mm, and may be a separation membrane of a material selected from the group consisting of polystyrene, polyether, polyethylene, and polypropylene.

According to another embodiment, the oil water separator ball has an average diameter of 100 ㎛ to 1,000 ㎛, as a channel-shaped pores is formed to the other surface from one surface of the separation membrane, the separation membrane unit surface area of 1 cm 2 10 one to per-thousand Density < / RTI >

According to another embodiment of the present invention, the organic solvent discharge port and the fermentation liquid discharge port may be equipped with a back pressure regulator at a lower end thereof.

Further, according to the present invention,

An organic acid production fermenter for containing a microorganism culture liquid for performing organic acid fermentation;

An extractor for extracting the organic acid from the microbial culture liquid transferred from the organic acid production fermenter using an organic solvent;

An oil-water separator as described above for separating a mixture of the fermentation broth and the organic solvent transferred from the extractor;

A fermentation liquid transfer line for transferring the fermentation liquid from which the organic acid has been extracted from the oil water separator to the organic acid production fermenter; And

An organic solvent catalytic reactor for separating the organic acid from the organic solvent containing the extracted organic acid

The present invention also provides an apparatus for producing organic acids by microbial organic acid fermentation.

According to an embodiment of the present invention, the organic acid extraction reaction in the extractor is carried out at a pressure of 5 bar to 50 bar and at a temperature of 4 ° C to 40 ° C for 10 minutes to 20 minutes, Lt; / RTI > under the following stirring conditions.

According to another embodiment of the present invention, the organic solvent may be selected from the group consisting of butyl butyrate, dodecanol, oleyl alcohol, and mixtures thereof.

According to another embodiment of the present invention, the organic acid may be selected from the group consisting of butyric acid, hexanoic acid, and mixtures thereof.

According to another embodiment of the present invention, the microorganism may be a single microorganism or a mixed microorganism producing an organic acid.

According to the present invention, there is provided a water-oil separator for an organic acid production apparatus by microbial organic acid fermentation, which can easily separate organic acids extracted by an organic solvent from the fermentation broth without any additional solvent or additional process, Can be provided.

1 is a diagram showing a conventional organic acid fermentation process.
2 is a cross-sectional view of the oil water separator according to the present invention.
3 is an enlarged view of only the separator portion of the oil water separator according to the present invention.
4 is an enlarged view of only a binding groove portion of a separation membrane portion of a water-oil separator according to the present invention.
5A and 5B are photographs of an oil-water separator 5a according to the present invention and an organic acid production device 5b including such an oil-water separator, respectively.

BRIEF DESCRIPTION OF THE DRAWINGS The objectives, specific advantages and novel features of the present invention will become more apparent from the following detailed description taken in conjunction with the accompanying drawings, in which: FIG. It should be noted that, in the present specification, the reference numerals are added to the constituent elements of the drawings, and the same constituent elements are assigned the same number as much as possible even if they are displayed on different drawings. Also, the terms "first "," second ", and the like are used to distinguish one element from another element, and the element is not limited thereto. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS In the following description of the present invention, detailed description of related arts which may unnecessarily obscure the gist of the present invention will be omitted.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

The oil-water separator for an apparatus for producing organic acids by microbial organic acid fermentation according to the present invention comprises the following components,

And a transporting space for transporting the mixed liquid from one end to the other end, wherein the central axis passing through the center of the cross section of the one end and the center of the cross section of the other end are formed on different axis And an area of the transverse section at the other end is larger than an area of the transverse section at the one end;

A separation membrane of a porous hydrophobic material fixed to the transverse side of the one end of the transport space in a membrane form and having a plurality of water separation holes formed therein to allow the mixed solution to pass therethrough;

An inlet port disposed at one end of the oil-water separation vessel for introducing the mixed fluid into the transportation space;

An organic solvent discharge port disposed at the other end of the oil-water separation vessel for discharging an organic solvent separated from the fermentation broth and containing the extracted organic acid to the outside; And

And a fermentation liquid discharge port disposed at the other end of the oil-water separation vessel for separating the organic solvent from the organic solvent and discharging the fermentation liquid to the outside.

2 is a cross-sectional view of an oil-water separator 200 according to the present invention. Referring to FIG. 2, an oil-water separator 200 includes an oil-water separation vessel 210 formed in a generally cylindrical shape, And can be manufactured in various forms such as a bar, a rectangular barrel, and a cylinder that can be manufactured in a free shape.

In the oil-water separating vessel 210, a space 221 for containing a mixed solution of a fermentation broth and an organic solvent containing an organic acid is bounded by a separation membrane 220, an organic solvent containing an organic acid- And a space 222 in which the first and second electrodes 222 and 222 are separated and accommodated. As described above, the mixed liquid in the form of an emulsion introduced from the extractor is transported from one end of the oil water separating vessel 210 to the other end direction (the mixed liquid is transported from the left to the right in the drawing).

In the present invention, the separation of the organic acid-extracted fermentation broth and the extracted organic acid-containing organic solvent is easier because the unique shape of the water separation vessel 210 according to the present invention and the fermentation broth containing the organic acid And the arrangement of the separation membrane 220 disposed on the side of the space 221 for receiving the mixed liquid in which the organic solvent is mixed.

That is, in the present invention, unlike the conventional oil water separator, the separation membrane 220 of the porous hydrophobic material is included in the transportation space inside the oil water separator. By this separation membrane 220, The organic acid extracted by the organic solvent during fermentation can be easily separated from the fermentation broth with higher efficiency.

Specifically, in the step of producing various organic compounds such as organic acids by fermentation, an organic solvent for extracting organic acids and a fermentation liquid for extracting organic acids are mixed and contacted inside the extractor. At this time, In order to accomplish this, a strong stirring and other operations are inevitably involved. At this time, an emulsion mixture of the fermentation broth and the organic solvent is generated in accompaniment with the stirring, and even if the emulsion mixture is transferred to the next water separation step, the efficiency of the water separation efficiency is inevitably lowered. Conventionally, in order to suppress such emulsion formation, an excess amount of an organic solvent has been used. However, in this case, a large amount of expensive organic solvent is used, which causes problems such as an increase in processing cost.

Therefore, in order to solve the above problems, the present invention adopts a special structure for smooth separation of the fermentation broth and the organic solvent in the oil water separator, which is fixed inside the oil separator in the form of a membrane, and the mixed solution of the fermentation broth and the organic solvent A porous hydrophobic material separation membrane 220 is formed. The separation membrane 220 functions to separate the fermentation broth (emulsion breaker) and the organic solvent layer by destroying the emulsion mixture comprising the fermentation broth and the organic solvent. This emulsion breaker function is possible because the separation membrane 220 according to the present invention is made of a porous hydrophobic material, and the emulsion is destroyed by the following principle.

That is, when the emulsion passes through the inside of the separation membrane 220, the separation membrane 220 has a hydrophobic group on the surface of the inside of the pores through which the emulsion passes. Therefore, the surface of the separation membrane 220 contacting the emulsion and the organic solvent component The interfacial tension between the hydrophobic groups is lowered. Therefore, wetting of the separation membrane 220 by the organic solvent component occurs, and the fermentation liquid in the two components of the emulsion, that is, the fermentation broth and the organic solvent component, continuously passes through the separation membrane 220, The components are wetted by the separation membrane 220. This wetting phenomenon occurs when the organic solvent component that damps the separation membrane 220 reaches a certain limit, it flows out from the separation membrane 220 in the form of a relatively large droplet. Due to the difference in specific gravity of the organic solvent liquid, And the organic solvent containing the fermentation broth and the organic acid are separated and moved to the upper layer of the space 222.

Further, in the present invention, in order to further facilitate the separation of the organic solvent containing organic acid from the fermentation broth separated by the separation membrane 220, the organic solvent containing the organic acid-extracted fermentation broth and the organic acid is separated The subsequent space 222 is configured to be larger than the space 221 before the separation.

This is because the liquid after passing through the separation membrane 220, that is, the organic solvent containing the extracted organic acid and the fermentation liquid from which the organic acid is extracted, passes through the fermentation liquid discharge port and the organic solvent discharge port, The fermentation liquid having relatively high specific gravity should be discharged to the outside and the organic solvent having a relatively low specific gravity will be discharged to the organic solvent discharge port through the fermentation liquid discharge port disposed at the lower end of the oil water separator 200, The other end of the oil water separating vessel 210, that is, the side indicated by reference numeral 222 in FIG. 2, is connected to one end of the oil water separating vessel 210, that is, the side indicated by reference numeral 221 in FIG. 2 There is a need to have a larger volume.

In this case, under the assumption that the pressure inside the oil water separating vessel 210 is kept constant by the Bernoulli's law, the velocity of the fluid (That is, on the side indicated by reference numeral 221 in Fig. 2) and on the other side where the cross-sectional area of the cross section is larger (i.e., on the side indicated by reference numeral 222 in Fig. 2). As a result, due to such a speed difference, after passing through the separation membrane 220, that is, after the fermentation broth and the organic solvent have been separated, sufficient time is given to the separated two liquids, To be more fully separated.

At this time, in order to allow the separation mechanism to work more smoothly, the cross section of the other end may be made 2 to 9 times the area of the cross section of the one end. When the cross section of the other end is less than twice the area of the cross section of the one end, the effect according to the Bernoulli's law described above can not be sufficiently obtained, and if it exceeds 9 times, the space of the other end is inefficient This makes it difficult to operate in a high pressure state or the residence time is prolonged to induce emulsification of the fermentation broth and the organic solvent, which makes it difficult to recover the organic solvent.

The volume of the one end and the other end of the oil separator 210 may be varied in accordance with the length of the oil separator 210 in the fluid flow direction. The volume of the other end may be twice the volume of the one end To 18 times. If the volume of the other end is less than twice the volume of the one end, there is a possibility of being discharged to the culture liquid discharge port and the organic solvent discharge port in a state in which the culture liquid and the organic solvent are not sufficiently separated. The emulsion is regenerated depending on various properties of the organic solvent and the culture liquid due to an increase in the residence time, and the size of the apparatus is excessively large, thereby lowering the price competitiveness of the apparatus.

It is preferable that a connecting portion between the one end and the other end increases in cross-sectional area from the one end toward the other end. If there is no connecting portion gently increasing the cross-sectional area from the one end to the other end, It is undesirable because there is a problem such as an increase in differential pressure and vortex generation. At this time, the volume of the connection portion may be 1.5 to 2.5 times the volume of the one end.

As described above, the oil-water separation vessel 210 according to the present invention is divided into two spaces based on the separation membrane 220, and the location where the separation membrane 220 of the porous hydrophobic material is installed, The separating membrane 220 may be located at a position which is one third to one-half of one end of the oil-water separating vessel 210, based on the transport direction of the fermentation liquid inside the separating membrane 210, That is, near the inlet port 230, the mixed liquid flowing into the separation membrane is directly introduced into the separation membrane, so that it can not be uniformly introduced into the entire separation membrane, so that the separation membrane can not be utilized efficiently as a whole. The organic solvent outlet port 240 or the fermentation liquid outlet port 250, there is a time margin for separating the solvent and the fermentation liquid. It is not preferable because the separation efficiency is lowered because it is not divided.

In the oil water separator 200 according to the present invention shown in FIG. 2, the oil water separating vessel 210 can be divided into an upper portion and a lower portion based on a position where the separator 220 is installed. The upper and lower portions may be assembled by bolt-nut pairs after mounting the separation membrane 220. Also, a port 271 for discharging the gas before the operation of the oil water separator 200, for injecting the solvent for washing, and a port 272 for draining water from the inside of the oil water separating vessel 210 may be provided , And each of these ports is equipped with a needle valve. Further, a back pressure regulator is installed at the lower end of the solvent discharge port 240 and the fermentation liquid discharge port 250 to maintain the pressure inside the oil separation container 210 constant, It facilitates the discharge of fermentation liquid. For example, the operation of the rear pressure regulator is as follows. That is, if the pressure inside the water separating vessel 210 is set to 50 bar, the pressure of the rear pressure regulator is locked when the pressure is 50 bar or less, but the pressure of the interior increases (when the gas or solvent is supplied from the outside) bar, the controller is opened and the solvent or the fermentation liquid is discharged. Particularly, this is not a simple on / off method but a gradual opening / closing method, so that the solvent or the fermentation liquid is suddenly discharged and the interface between the solvent and the fermentation broth is prevented from being broken.

A predetermined pressure is applied to the water-oil separator 200 according to the present invention, and a flow of fluid is generated in the oil-water separation vessel 210 by the pressure, so that a certain pressure is applied to the separation membrane 220 . Therefore, the front pressure gauge 261 and the rear pressure gauge 262 are installed on the basis of the position where the separation membrane 220 is installed, so that the pressure inside the oil water separation vessel 210 can be adjusted to an appropriate level. Further, means for fixing the separation membrane at a predetermined position on the inner circumferential surface of the oil water separating vessel 210 is necessary in order to resist the pressure applied to the separation membrane 220. Accordingly, the oil-water separating vessel 210 may include a binding groove 223 which is recessed in the inner circumferential surface of the separating membrane 220 so that the edge of the separating membrane 220 is inserted and fixed. FIG. 3 is an enlarged view of only the separator 220 of the oil-water separator 200 according to the present invention, and FIG. 4 is an enlarged view of only the separator 220 in FIG. . 3 and 4, the rim of the separation membrane 220 is seated inside the binding groove 223, so that it can be fixed at a predetermined position despite the flow of the fluid.

Meanwhile, it is also possible to use a separate support 224 so that the separation membrane 220 can be more firmly fixed. The support 224 is formed in the shape of a ring having a hollow at its center and is disposed inside the binding groove 223 and has at least one side edge of the separation membrane 220 or at least one side edge of the separation membrane 220 And can adhere to any one or more of them to support the separation membrane.

In the present invention, the separation membrane may be a separation membrane of a hydrophobic material selected from the group consisting of polystyrene, polyether, polyethylene, and polypropylene, And may be a channel-shaped pore formed from one surface to the other surface of the separation membrane, for smooth fluid communication, having a diameter of 100 mu m to 1,000 mu m. When the average diameter of the water separation holes formed in the separation membrane is less than 100 탆, the flow resistance is increased and the flow rate of the fluid is lowered and the separation rate is lowered or the pressure loss is increased. There is a problem that the fine emulsion which has not passed through the raw separation membrane passes through as it is and the separation efficiency is lowered. Further, it is preferable that an oil-water separation hole is formed at a proper density on the surface of the separation membrane, and the pores may be formed at a density of 10 to 1,000 per 1 cm 2 of the separation membrane unit surface area. When the pores are formed with a density less than the above range, there is a problem that the efficiency is not sufficiently exhibited as a so-called emulsion breaker. If too much pores are formed, the mechanical strength of the separation membrane may be lowered . Meanwhile, the thickness of the separation membrane may be 10 mm to 300 mm, which is also a numerical value considering the efficiency as an emulsion breaker and the mechanical strength of the separation membrane.

Further, according to the present invention,

An organic acid production fermenter for containing a microorganism culture liquid for performing organic acid fermentation;

An extractor for extracting the organic acid from the microbial culture liquid transferred from the organic acid production fermenter using an organic solvent;

An oil-water separator as described above for separating a mixture of the fermentation broth and the organic solvent transferred from the extractor;

A fermentation liquid transfer line for transferring the fermentation liquid from which the organic acid has been extracted from the oil water separator to the organic acid production fermenter; And

An organic solvent catalytic reactor for separating the organic acid from the organic solvent containing the extracted organic acid

The present invention also provides an apparatus for producing organic acids by microbial organic acid fermentation.

5A and 5B are photographs of the oil-water separator 5a according to the present invention and the organic acid production apparatus 5b including such an oil-water separator. Referring to FIG. 5A, there is shown a water separator having various pressure gauges and valves and having a wider diameter toward the bottom. Referring to FIG. 5B, An organic acid production fermenter on the left side, an extractor on the upper side, and an organic solvent catalytic reactor on the upper right side, and fluid delivery lines for fluidly connecting the respective reactors are shown.

The apparatus for producing organic acids according to the present invention comprises essential components of an apparatus for producing organic acids in addition to the oil-water separator according to the present invention described above, that is, an organic acid production fermenter in which an organic acid fermentation reaction is performed by microorganisms, An organic solvent-catalytic reactor for separating the organic acid from the organic solvent containing the extracted organic acid, and a feeder line for feeding the fermentation liquid from which the organic acid has been extracted to the fermenter. Each of these configurations can be referred to the description of FIG. 1 described above.

Meanwhile, the apparatus for producing organic acids according to the present invention is particularly suitable for the case of using an extractor in which high pressure and stirring conditions are applied for extracting organic acids. In this case, the generation of emulsion is particularly frequent between the fermentation broth and the organic solvent . Therefore, in the present invention, the organic acid extraction reaction in the extractor is carried out at a pressure of 5 bar to 50 bar and at a temperature of 4 ° C to 40 ° C for 10 minutes to 20 minutes, Under agitation conditions.

The apparatus for producing organic acids according to the present invention is suitable for producing various organic acids, but organic acids selected from the group consisting of butyric acid, hexanoic acid and mixtures thereof can be produced according to the present invention. In order to produce the organic acid, it is possible to use the organic acid producing microorganism known in the art alone, but it is also possible to use a mixed microorganism of such organic acids. Further, although not limited thereto, the organic solvent used for organic acid extraction may be selected from the group consisting of butyl butyrate, dodecanol, oleyl alcohol, and mixtures thereof.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the present invention. It is obvious that the modification or improvement is possible. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

Claims (14)

And a transporting space for transporting a mixed solution in which a fermentation liquid containing an organic acid and an organic solvent for extracting the organic acid are transported from one end to the other end of the transporting space, Wherein a center axis passing through the center of each of the transverse sections is disposed on a different axis and an area of the transverse section at the other end is formed to be larger than an area of the one transverse section;
A separation membrane of a porous hydrophobic material fixed to the transverse side of the one end of the transport space in a membrane form and having a plurality of water separation holes formed therein to allow the mixed solution to pass therethrough;
An inlet port disposed at one end of the oil-water separation vessel for introducing the mixed fluid into the transportation space;
An organic solvent discharge port disposed at the other end of the oil-water separation vessel for discharging an organic solvent separated from the fermentation liquid and extracted and containing the organic acid; And
And a fermentation liquid discharge port disposed at the other end of the oil-water separation vessel for separating the organic solvent from the organic solvent and discharging the fermentation liquid to the outside,
Wetting occurs on the surface of the separation membrane, and the organic solvent in the mixture mixture flows out through the separation membrane in the form of droplets,
The droplet-form organic solvent and the fermentation liquid are separated due to a difference in specific gravity, the organic solvent moves to an upper portion of the transport space, the fermentation liquid moves to a lower portion of the transport space,
Wherein the organic solvent outlet port is formed in an upper portion of the transporting space and the fermentation liquid outlet port is formed in a lower portion of the transporting space,
Wherein an area of the cross section of the other end is larger than an area of the cross section of the other end so that the moving speed of the fermentation liquid and the organic solvent separated through the separation membrane is relatively slower than the moving speed of the mixed liquid moving in the direction of the separation membrane 2 to 9 times,
Oil - Water Separator for Organic Acid Production by Microbial Organic Acid Fermentation. delete The oil separator for organic acid production apparatus according to claim 1, wherein the volume of the other end is 2 to 18 times the volume of the one end. 2. The microorganism according to claim 1, wherein a connecting portion between the one end and the other end increases in cross-sectional area from the one end to the other end, and the volume of the connecting portion is 1.5 to 2.5 times the volume of the one end. Oil - Water Separator for Organic Acid Production by Organic Acid Fermentation. The method according to claim 1, wherein the separation membrane is fixed within a range of one-third to one-half of the one end of the oil-water separation vessel based on the direction of transport of the fermentation liquid inside the oil- Oil - Water Separator for Organic Acid Production by Organic Acid Fermentation. [2] The apparatus according to claim 1, wherein the oil-water separation vessel comprises: a coupling groove recessed in an inner circumferential surface of the separation membrane so that a rim of the separation membrane is inserted and fixed; And at least one of a rim of the one side of the separation membrane or a side of the other side of the separation membrane, which is disposed inside the binding groove, and which supports the separation membrane, Wherein the microbial organic acid fermentation is carried out in the presence of an organic solvent. The organic acid production apparatus according to claim 1, wherein the separation membrane has a thickness of 10 mm to 300 mm and is a separation membrane of a material selected from the group consisting of polystyrene, polyether, polyethylene, and polypropylene Oil separator. The method of claim 1, wherein the water separation ball has an average diameter of 100 ㎛ to 1,000 ㎛, formed with a channel-shaped pores is formed to the other surface from one surface of the separation membrane, the separation membrane unit surface area of 1 cm 10 one to one thousand density per 2 Wherein the organic acid is produced by the microbial organic acid fermentation. The oily water separator according to claim 1, wherein the organic solvent discharge port and the fermentation liquid discharge port are equipped with a back pressure regulator at a lower end thereof. An organic acid production fermenter for containing a microorganism culture liquid for performing organic acid fermentation;
An extractor for extracting the organic acid from the microbial culture liquid transferred from the organic acid production fermenter using an organic solvent;
An oil-water separator according to any one of claims 1 to 9 for separating a mixture of the fermentation broth and the organic solvent transferred from the extractor;
A fermentation liquid transfer line for transferring the fermentation liquid from which the organic acid has been extracted from the oil water separator to the organic acid production fermenter; And
An organic solvent catalytic reactor for separating the organic acid from the organic solvent containing the extracted organic acid
Wherein the organic acid is produced by microbial organic acid fermentation. [Claim 11] The method of claim 10, wherein the organic acid extraction reaction in the extractor is performed at a pressure of 5 bar to 50 bar and at a temperature of 4 to 40 DEG C for 10 to 20 minutes by stirring the microorganism culture solution and the organic solvent at 500 rpm or less Wherein the organic acid is produced by reacting the organic acid with a microorganism. [Claim 11] The apparatus for producing organic acid by fermentation of microbial organic acids according to claim 10, wherein the organic solvent is selected from the group consisting of butyl butyrate, dodecanol, oleyl alcohol and mixtures thereof. [Claim 11] The apparatus for producing organic acid by fermentation of microbial organic acids according to claim 10, wherein the organic acid is selected from the group consisting of butyric acid, hexanoic acid, and mixtures thereof. [Claim 11] The apparatus for producing organic acid by fermentation of microorganism organic acid according to claim 10, wherein the microorganism is a sole microorganism or a mixed microorganism that produces an organic acid.
KR1020150060357A 2015-04-29 2015-04-29 Oil-water separator for production of organic acid through organic acid fermentation by microorganism having improved oil-water separation efficiency by having specific three dimensional structure, and a device for production of organic acid including the same KR101697863B1 (en)

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