KR101691729B1 - Plant culture container for evaluating phytotoxicity and method for evaluating phytotoxicity using the same - Google Patents
Plant culture container for evaluating phytotoxicity and method for evaluating phytotoxicity using the same Download PDFInfo
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- KR101691729B1 KR101691729B1 KR1020150059797A KR20150059797A KR101691729B1 KR 101691729 B1 KR101691729 B1 KR 101691729B1 KR 1020150059797 A KR1020150059797 A KR 1020150059797A KR 20150059797 A KR20150059797 A KR 20150059797A KR 101691729 B1 KR101691729 B1 KR 101691729B1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S426/00—Food or edible material: processes, compositions, and products
- Y10S426/809—Food or edible material: processes, compositions, and products including harvesting or planting or other numerous miscellaneous processing steps
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Abstract
The present invention relates to a container, And a lid part that is hermetically bonded to the container part, wherein the bottom surface of the container part is transparent, the side surface of the container part is opaque, and the top surface of the lid part is transparent. .
Description
The present invention relates to a plant growth vessel for evaluating phytotoxicity and a method for evaluating phytotoxicity using the same.
Soil plays a role as a final reservoir with the oceans in the process of global material circulation, and the contaminants introduced into the soil affect the surrounding ecosystem over a long period depending on the species, and eventually become persistent in human health and economic utilization Will influence. In particular, soil is a heterogeneous medium composed of various inorganic and organic components unlike water quality and atmospheric environment. It is predicted in relatively homogeneous water quality and atmospheric environment due to physical and chemical interaction between various soil constituents and pollutants. There is a difference between the results of the risk assessment. In addition, even if the soil is contaminated with the same concentration, if the characteristics of the soil itself, such as various physical and chemical characteristics and weathering degree of the soil constituting material, are changed, the result of the risk evaluation also shows a difference. As a result, a number of evaluation methods have been developed using a variety of biological species such as plants, animals, insects and microorganisms that live on the soil as an integrated evaluation method using receptors (or specimens) directly affected by the toxicity of soil pollution The germination rate of plant seeds, which is relatively easy to organize the management and testing facilities, and the evaluation method using the difference in growth rate of roots are mainly used. As a method for evaluating the phytotoxicity of chemical substances among such conventional evaluation methods, the test methods suggested by US EPA and OECD are used as representative evaluation methods. The method recommended by the existing US EPA and OECD consists of measuring the germination rate of plant seeds and root length of germinated seeds in an aqueous solution containing pollutants and is based on germination of plant seeds in aqueous solution, The results of the method are not verified to be applied to the soil environment. Actually, the behavior of chemicals in water environment and soil environment is known to be very different.
Therefore, the conventional methods for evaluating the risk of contaminated soil using plant seed have the following problems. First, in the conventional evaluation method, evaluation is carried out using a transparent aqueous solution or a gel-type medium containing contaminants at a specific concentration so that the root growth of plant seeds can be easily observed. However, The complex effects of biological constituents and biological effects caused by the physical and chemical reactions occurring between the various contaminants are not taken into account and thus the difference between the results of the evaluation in the laboratory and the actual field . Second, when the conventional evaluation method is carried out in an opaque medium such as soil, in order to evaluate the change in the root growth of the plant seed, it is necessary to carry out only one time at the end of the evaluation. If the evaluation is continuously performed In this case, it is necessary to increase the test object and the test port according to the number of evaluations. Third, the conventional evaluation method performed in the aqueous solution of the pollutant can be performed by 1) measuring the germination rate of the seed, or 2) measuring the stem and root length in the early stage of plant growth, Since the evaluation of the dead seed is not carried out and the evaluation is carried out within a short period of time (within about one week), the change due to the physiological characteristics such as the volume growth of the plant roots caused by the longer growth period of the plant seeds It is difficult to do.
Korean Patent Laid-Open Publication No. 2013-0053524 discloses a method for evaluating toxicity using a plant germination test in an aqueous solution of heavy metals and an empirical equation for estimating the concentration of heavy metals in the soil. However, the root of the plant according to the degree of soil contamination is non- It has a limit that can not be measured. Korean Patent Laid-Open Publication No. 2013-0053524 discloses a method for evaluating toxicity using a plant germination test in a heavy metal aqueous solution and an empirical equation for estimating the concentration of heavy metals in the soil. However, Germination and root length of the plant are different from the growth of plant roots in actual soils where complex pollutants are present, depending on the empirical formula for measuring the elongation of the plant, and the roots of the plant according to the soil contamination can be measured nondestructively and continuously There is no limit.
Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made in view of the above problems, and it is an object of the present invention to provide a plant growth vessel capable of continuously measuring the roots of a plant growing in a plurality of mediums in a non-destructive manner over time, The purpose is to provide.
In order to solve the above-described problems, And a lid part that is hermetically bonded to the container part, wherein the bottom surface of the container part is transparent, the side surface of the container part is opaque, and the top surface of the lid part is transparent. Lt; / RTI >
In addition, the present invention provides a method for evaluating phytotoxicity using a plant growth vessel for evaluating the phytotoxicity.
According to the plant growth container for evaluating phytotoxicity of the present invention and the method for evaluating phytotoxicity using the same, it is possible to obtain a root image which is closely adhered to the transparent bottom surface of the plant growth container in the opaque test medium, It is possible to evaluate the phytotoxicity of the test medium by non-destructive and continuous measurement through a collection device.
FIG. 1 is a cross-sectional view illustrating the growth process of roots along the bottom surface of a plant growth vessel during germination and growth of plant seeds according to the present invention.
2 is a cross-sectional view of a plant toxicity assessment system for non-destructive continuous measurement of plant roots using an image collection device according to an embodiment of the present invention.
Fig. 3 is an external view of a plant growth container holder according to an embodiment of the present invention. Fig.
FIG. 4 is a result of observing the root growth of plants by time of day using the plant growth container of the present invention.
FIG. 5 is a diagram illustrating the process of detecting plant roots and measuring the length and area of plant roots from scanned images using image processing software.
Fig. 6 is a diagram showing the root length in the contaminated soil 1 to 10 as compared with the root length growth in the non-contaminated soil, as measured using the plant growth vessel of the present invention.
The present invention relates to a plant growth vessel for evaluating phytotoxicity and a method for evaluating phytotoxicity using the plant growth vessel. Specifically, the present invention relates to a method for evaluating contaminated soil toxicity using plant root roots growth in the germination and growth stages of plant seeds, and relates to a method for evaluating the toxicity of roots of plants growing in non-homogeneous opaque medium such as soil by non-destructive methods And to a method for evaluating phytotoxicity using the plant growth vessel. BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a plant growth regulator for evaluating phytotoxicity,
The plant growth vessel for evaluating phytotoxicity of the present invention comprises: a container part; And a lid part hermetically coupled to the container part, wherein the bottom surface of the container part is transparent, the side surface of the container part is opaque, and the top surface of the lid part is transparent. And the side surface of the container portion is opaque and the side surface of the container is not lighted. The bottom surface of the container portion is transparent and the growth of the root portion of the plant is observed. And the upper surface of the lid part is transparent, so that the photosynthetic property of the plant top part is easy.
Plant seeds germinate as the embryo begins to grow when environmental conditions such as moisture, temperature and air are met. At the beginning of seed germination stage, roots grow first, followed by stem and leaf. At this time, the developing stems and leaves can be measured by various methods by growing in the upper part of the medium, but the growth process of the root part of the plant growing inside the medium is difficult to be visually observed. However, the root portion of the plant has a positive roughening ability to grow in the direction of gravity, and thus the root portion of the plant is brought into contact with the bottom surface of the plant growth vessel of the present invention and then grows close to the bottom surface.
The growth of plant roots depends on the ability to obtain water and inorganic salts in a direct smile ecosystem surrounding roots called rhizosphere. If there are contaminants that cause phytotoxicity in the rhizosphere, the growth of roots is inhibited. Accordingly, the bottom surface of the plant growth vessel is a monitor having a constant area, and the roots of the growing plant gradually fill the monitor of a certain area during the growing process, and the area of the plant roots filling the bottom surface varies depending on the conditions of the medium do. The medium filled in the plant growth vessel used is mainly opaque in light brown on black, and the roots of the plant seeds become white. Therefore, by using the image collecting device, the root of the plant Can be measured.
The plant growth vessel for evaluating phytotoxicity of the present invention may further include a plant growth vessel holder for placing a single or multiple plant growth vessels in the vessel. The apparatus may further include an image collection device for collecting a growth image of the subject plant and an image collection device control for controlling the image.
The plant of the present invention is not particularly limited, but it is preferable to use a kind of dicotyledonous plant group such as radish, sunflower, soybean, and rapeseed which is easy to observe the growth of the main muscle at an initial stage of germination and a plant family.
The medium of the present invention may be a material composed of a particulate material, such as artificial soil, sludge, sediments, soil treated with an improving agent, so that plant roots can grow in the direction of gravity, preferably soil.
According to another aspect of the present invention, there is provided a method for evaluating phytotoxicity using a plant growth vessel, comprising the steps of: placing a seed of a plant to be evaluated in each of a contaminated medium and a non-contaminated medium in a container portion of the plant growth vessel; Germinating seeds of said plant; And comparing the germination rate, the root length of the plant, or the root growth rate of the seed of the plant with respect to the contaminated medium and the non-contaminated medium, respectively.
The germination rate (
(Where N G is the number of plant seeds with a root length of at least 1 cm, and N T is the total number of seeds used).
Further, the plant root length (L R ) may be calculated according to the following formula.
(Where S L is the root length of the plant growth vessel bottom surface and S H is the height from the bottom surface to the plant seed).
The root growth area ratio (R G ) may be calculated according to the following equation.
(Where R A is the area occupied by the root portion and B A is the total floor area of the plant growth vessel).
DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.
BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a perspective view of a plant toxicity evaluation apparatus according to an embodiment of the present invention. FIG. .
In order to carry out the method of the present invention, the
The plant growth container holder (6) processed so that a single or a plurality of plant growth vessels can be mounted is placed in an incubator (7) adjusted to match the germination condition of the plant seeds, wherein the germination condition of the plant seeds Adjust to the appropriate level according to the characteristics.
The plant growth vessel holder (6) is advantageously manufactured in a white or gray system for image acquisition and image processing and image analysis convenience.
An image is acquired using an
At this time, the germination rate of the plant seed, the length of the root of the plant, and the growth rate of the root are calculated by the image analysis.
The germination rate (G R ) is calculated as follows.
Where N G is the number of plant seeds with a root length of at least 1 cm and N T is the total number of seeds used in the test.
The root length (L R ) is calculated as follows.
Where S L is the length of the root at the bottom of the plant growth vessel and S H is the height from the bottom to the seed.
The root growth rate (R G ) is calculated as follows.
Where R A is the area occupied by the root portion and B A is the total floor area of the plant growth vessel.
The germination rate (G R ), root length (L R ) and root growth area ratio (R G ) measured here are compared with the control to evaluate the phytotoxicity of the opaque heterogeneous medium such as contaminated soil.
According to the plant growth container for evaluating phytotoxicity of the present invention and the method for evaluating phytotoxicity using the same, it is possible to obtain a root image which is closely adhered to the transparent bottom surface of the plant growth container in the opaque test medium, It is possible to evaluate the phytotoxicity of the test medium by non-destructive and continuous measurement through a collection device.
Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples. These Examples and Experiments are only for illustrating the present invention, and thus the scope of the present invention is not construed as being limited by these Examples and Experimental Examples.
Example 1: Measurement of roots growth change with time using the container of the present invention
Using the vessel of the present invention, the growth of the root of the plant was observed at the lower part of the vessel with respect to Brassica juncea over time, and the results are shown in Fig. The processing of images obtained from the lower end of the container of the present invention is shown in Fig. Specifically, FIG. 5 is a process for detecting plant roots and measuring length and area of plant roots from scanned images using image processing software.
Experimental Example 1: Evaluation of Inhibition of Root Length Growth of Various Contaminated Soils Using the Container of the Present Invention
Using the present invention, plant root length growth inhibition evaluation was carried out using seeds of Raphanus sativus, in contaminated soils 1 to 10 contaminated with various concentrations and substances.
The contaminated soil 1 to 10 are the samples collected near the abandoned mines, respectively, and the types and concentrations of the polluted heavy metals are different from each other. The contaminated soil 1 to 4 (S1 to S4) and the contaminated
(mg / kg)
(mg / kg)
When the root length growth in the non-contaminated soil was 1, the root length in each of the contaminated soil 1 to 10 was shown in Fig. Referring to FIG. 6, through the present invention, inhibition of plant root length growth in contaminated soil can be confirmed by quantitative comparison.
DESCRIPTION OF THE REFERENCE SYMBOLS
1: cover part of the plant growth vessel
2: container portion of plant growth vessel
3. Plant growth vessel
4: plant seed
5: Plant growth medium
6: Plant growth vessel holder
7: incubator
8: incubator controller
9: Image acquisition device
10: Image acquisition device controller
11: plant growth vessel inserting section
Claims (7)
Positioning the seed of the plant to be evaluated for each of the contaminated medium and the non-contaminated medium in the container portion of the plant growth vessel;
Germinating seeds of said plant; And
The roots grown along the transparent bottom surface of the container portion of the plant growth container were observed,
Comparing the germination percentage of the seed of the plant, the root length of the plant, or the root growth rate of the plant with respect to the contaminated medium and the non-contaminated medium, respectively,
The plant growth vessel comprises a container portion; And a lid part that is hermetically engaged with the container part. The plant growth container for non-destructive and continuous evaluation of phytotoxicity,
Wherein the bottom surface of the container portion is transparent to observe the roots of the plant growing along the bottom surface, the side surface of the container portion is opaque, and the top surface of the lid portion is transparent.
The germination rate (
(Where N G is the number of plant seeds with a root length of at least 1 cm, and N T is the total number of seeds used).
Wherein the plant root length (L R ) is calculated according to the following equation.
(Where S L is the root length of the plant growth vessel bottom surface and S H is the height from the bottom surface to the plant seed).
Wherein the root growth area ratio (R G ) is calculated according to the following equation.
(Where R A is the area occupied by the root portion and B A is the total floor area of the plant growth vessel).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020150059797A KR101691729B1 (en) | 2015-04-28 | 2015-04-28 | Plant culture container for evaluating phytotoxicity and method for evaluating phytotoxicity using the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020150059797A KR101691729B1 (en) | 2015-04-28 | 2015-04-28 | Plant culture container for evaluating phytotoxicity and method for evaluating phytotoxicity using the same |
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| Publication Number | Publication Date |
|---|---|
| KR20160128056A KR20160128056A (en) | 2016-11-07 |
| KR101691729B1 true KR101691729B1 (en) | 2016-12-30 |
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| KR1020150059797A KR101691729B1 (en) | 2015-04-28 | 2015-04-28 | Plant culture container for evaluating phytotoxicity and method for evaluating phytotoxicity using the same |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101296509B1 (en) * | 2013-03-26 | 2013-08-13 | 주식회사 모뉴엘 | Plant manager |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6339517A (en) * | 1986-08-04 | 1988-02-20 | 森産業株式会社 | Plant tissue culture method and container |
| KR20100046408A (en) * | 2008-10-27 | 2010-05-07 | 류승윤 | Rotation-type plant cultivation container and manufacturing method for root observation |
| KR20130053524A (en) | 2011-11-14 | 2013-05-24 | 서울대학교산학협력단 | Method for evaluating phytotoxicity |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101296509B1 (en) * | 2013-03-26 | 2013-08-13 | 주식회사 모뉴엘 | Plant manager |
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