KR101686563B1 - Composition comprising extract of Eisenia bicyclis or its fraction for prevention or treatment of acne - Google Patents

Abstract

The present invention relates to a composition for preventing or treating acne comprising a rhubarb extract or a fraction thereof. The rhubarb extract and its fractions according to the present invention exhibit antimicrobial activity against acne bacteria and synergy with other antibiotics, and thus can be usefully used for prevention and treatment of acne.

Description

[0001] The present invention relates to a composition for preventing or treating acne, which comprises extracts of rhubarb or extract thereof,

The present invention relates to a composition for preventing or treating acne comprising an extract of rhubarb or a fraction thereof as an active ingredient.

Acne is a chronic inflammatory disease of the hair follicle gland, which is characterized by a variety of skin changes, including cotton (hardened sebaceous follicles in the follicle), papules (bulging skin lesions less than 1 cm in size), blisters, nodules, Sequelae can leave concave scars or enlarged scars. It is a disease that occurs in the face, neck, chest, etc. where sebaceous glands are gathered and inflammation occurs in sebaceous glands attached to hair follicles. The precise cause of acne is not known, but it is caused by multiple causes rather than one cause. One cause is the excessive secretion of sebaceous glands in puberty, and the epidermis of the hair follicle is keratoconjunctivitis (incomplete and inhomogeneous keratinization Abnormal keratinization), causing the hair follicles to clog and forming a basic lesion of acne (comedone, a hardened sebaceous gland). Among these, acne rash caused by bacteria is caused by the bacteria that reside in the hair follicle, especially Propionibacterium acnes.

Propionibacterium acnes is a bacterial bacterium that grows and feeds on sebum, causing it to become a major source of trouble. It is normally present in normal skin and does not cause any problems. However, when the hair follicles become clogged and sebum accumulates in the hair follicles, Eat quickly and grow up. After eating the fat (triglyceride), the fungus excretes a butter-like substance, fatty acids. Such a chemical change breaks the pH balance of the oil on the surface of the skin, alkalizing the weak acidity of the healthy skin, resulting in deterioration of the skin's own function and a vicious cycle of the skin.

In order to prevent and alleviate the acne induced by Propionibacterium acnes, a medicine mainly used or medicine for eating is used. It is a medicine made by transforming topical antibiotics (clindamycin, erythromycin) and vitamin A, which have a direct bactericidal effect against acne bacteria and prevent the production of free fatty acid. (Tretinoin, adrenalin), a strong antimicrobial agent, and acne ointment (benzoyl peroxide), which has a few anti-inflammatory and cotton-soluble effects, . There are two major types of drugs to eat: antibiotics that act to sterilize acne bacillus and reduce inflammation, and retinoids, which are known as roucutan, which are made by modifying vitamin A. Retinoids are known to regulate sebum secretion by reducing sebaceous secretion. However, this medication is not only used to control sebum, but also to treat abnormal keratinization (abnormal keratinization with incomplete and immature keratinization), inflammation reaction, bacterial growth It helps to block all pathways of acne development.

However, it is pointed out that such treatment improves the immune system against the antimicrobial agents of the gastrointestinal bacterium in addition to the side effects such as gastrointestinal disorder, liver and kidney damage, and giving birth to a birth defect in pregnant women. Therefore, there is an increasing interest in natural extracts having high antibacterial activity against Propionibacterium acnes.

On the other hand, Eisenia bicyclis is an arboreal plant of the kelp spruce, which has elongated leaves at the end of a single cylindrical stem, and a pocket of holly that grows from autumn to winter. The body turns dark brown or black when dry. It can be used for food, and it is also used as a substitute for kelp, in addition to a moth or aphid. It is also used as a raw material of alginic acid and contains a large amount of minerals such as iodine and potassium. They are distributed mainly in Korea (Ulleungdo, Dokdo) and Japan, and they live in the lower rocky area of the tidal flat within 10m depth.

As a result of intensive efforts to develop a therapeutic drug for acne, the present inventor has found through experimentation that rhubarb extract or its fractions have antimicrobial activity against acne bacterium Propionibacterium acnes, thereby completing the present invention.

It is an object of the present invention to provide a composition for preventing or treating acne comprising an extract of rhubarb or a fraction thereof as an active ingredient.

In order to achieve the above object, the present invention provides a composition for preventing or treating acne, comprising the rhubarb extract or the fraction thereof as an active ingredient.

The rhubarb extract and its fractions according to the present invention exhibit antimicrobial activity against acne bacteria and synergy with other antibiotics, and thus can be usefully used for prevention and treatment of acne.

The present invention provides a pharmaceutical composition for preventing or treating acne comprising an extract of rhubarb or a fraction thereof as an active ingredient.

Hereinafter, the present invention will be described in detail.

Eisenia bicyclis, an active ingredient of the present invention, is a perennial seaweed, and the rhubarb extract or its fractions according to the present invention can be produced according to a conventional method for producing an algae extract. Specifically, the extract can be obtained by a cold extraction method, Extraction method, and the like, and conventional extracting equipment, ultrasonic pulverizing extractor, or fractionating unit can be used, but the present invention is not limited thereto. Further, the extract or the fraction thereof may be subjected to filtration, concentration or drying to remove the solvent, and may be performed by filtration, concentration, and drying, but is not limited thereto. The dried rhubarb extract or its fraction may be used in powder form or the powder may be dissolved in distilled water or a conventional solvent.

The rhubarb extract according to the present invention can be obtained by extracting rhubarb with water or an alcohol having 1 to 4 carbon atoms, preferably an alcohol having 1 to 4 carbon atoms, more preferably a methanol extract.

In addition, the rhubarb fraction according to the present invention may be prepared by adding a rhubarb extract to a polar solvent such as methanol, ethanol, propanol or isopropanol, or a mixed solvent composed of at least two non-polar solvents such as ethyl acetate, n-hexane, dichloromethane or chloroform And it can be obtained by separately separating the reaction product with hexane, chloroform, ethyl acetate and n-butanol in sequence, more preferably ethyl acetate Lt; / RTI >

In the present invention, a rhubarb extract and its fractions were obtained in the following manner.

First, the crude powdery extract is extracted with an alcohol having 1 to 4 carbon atoms to obtain a crude extract. The obtained crude extract is extracted with hexane, chloroform, ethyl acetate, n-butanol ) To obtain a fraction.

The rhubarb extract or fraction thereof according to the present invention comprises a phlorotannin compound. The phlorotannin compound is a polyphenol compound having phloroglucinol as a basic constituent unit. The phloroglucinol compound is selected from the group consisting of eckol, fucofuroeckol-A (FF), 7-phloroeckol ) Dioxinodehydroeckol, phlorofucofuroeckol-A (PFF) and dieckol, and is preferably a fucofuroeckol-A ).

The rhodanine-derived phlorotannin compound of the present invention is an effective ingredient exhibiting an antibacterial effect against acne bacteria, and the acne bacteria may be mainly Propionibacterium acnes.

The composition of the present invention may further comprise one or more antibiotics. The antibiotic may be tetracycline, erythromycin, and lincomycin used in the treatment of acne, but is not limited thereto. Do not.

When the rhubarb extract of the present invention or a fraction thereof is used in combination with the antibiotic, it can exhibit a synergistic effect of inhibiting the growth of acne bacteria by interacting with the antibiotic. This means that the rhubarb extract or its fractions of the present invention are involved in inhibiting antibiotic resistance of acne bacteria

Accordingly, the rhubarb extract or its fractions according to the present invention exhibit antimicrobial activity against acne bacterium, and thus can be effectively used for prevention and treatment of acne.

In addition, the composition of the present invention may contain one or more known active ingredients having an effect of preventing or treating acne, together with the rhubarb extract or its fraction

The composition may further comprise a pharmaceutically acceptable carrier, excipient and diluent. The pharmaceutically acceptable carriers, excipients and diluents which are included in the composition include those conventionally used in the present invention and include lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, But are not limited to, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methylcellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. It is not. The pharmaceutical composition may further contain a lubricant, a wetting agent, a sweetening agent, a flavoring agent, an emulsifying agent, a suspending agent, a preservative, etc. in addition to the above components.

When the pharmaceutical composition of the present invention is formulated into a solid preparation for oral administration, solid preparations for oral administration include tablets, pills, powders, granules, capsules and the like. These solid preparations may contain at least one excipient For example, it is formulated by mixing starch, calcium carbonate, sucrose or lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. In addition, the pharmaceutical composition of the present invention may be formulated into a liquid preparation for oral administration. Liquid preparations for oral administration include suspensions, solutions, emulsions, syrups and the like. These liquid preparations may contain various excipients in addition to commonly used inert diluents (for example, purified water, GaIN / LPS, liquid paraffin) Wetting agents, sweetening agents, perfumes, preservatives, and the like. In addition, the pharmaceutical composition of the present invention may be formulated into preparations for parenteral administration, preferably intraperitoneal administration. Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. As the sterilized aqueous solution, a suitable buffer solution such as Hank's solution, Ringer's solution or physically buffered saline can be used. As the non-aqueous solvent, propylene glycol, polyethylene glycol, olive oil, The same vegetable oil, an injectable ester such as ethyl oleate, or the like can be used. If desired, preservatives, stabilizers, wetting or emulsifying agents, salts for controlling osmotic pressure and / or buffers may be used. On the other hand, in the case of suppositories, witepsol, macrogol, tween 61, cacao paper, laurin, glyceroglatin and the like may be used.

The term "administering" as used herein is meant to provide any desired composition of the invention to a subject in any suitable manner. The composition formulated in the above manner may be administered through various routes including parenteral or oral (transdermal, subcutaneous, intravenous, muscular, abdominal, etc.) in an effective amount. The "effective amount" as used herein refers to an amount that shows a preventive or therapeutic effect when administered to a patient. The dose of the composition according to the present invention can be appropriately selected depending on the route of administration, subject to be administered, age, sex, weight, individual difference, and disease state. Preferably, the composition of the present invention may contain the active ingredient in an amount of 0.1 to 10000 mg / kg body weight / day, more preferably 10 to 1000 mg / kg body weight / day, Can be administered in an effective amount. The composition of the present invention can be used alone or in combination with methods using surgery, radiation therapy, hormone therapy, chemotherapy and biological response modifiers for the prevention and treatment of acne.

Further, the present invention provides a cosmetic composition for prevention or improvement of acne, comprising the rhubarb extract or its fraction as an active ingredient.

At this time, the amount of the rhubarb in the cosmetic composition may be from 0.01 to 15% by weight of the total weight, but this can easily be determined by a person skilled in the art according to the product.

The cosmetic composition may be prepared by mixing the skin composition with external skin application agents such as water, physiological saline, glycerol, a surfactant, a moisturizer, a thickener, a chelating agent and a pigment, May be prepared in various forms, depending on the method of production. For example, the cosmetic composition may be in the form of an ointment, a cream, a softened longevity (skin), a convergent lotion (lotion), an emulsion, an astringent, a massage cream, , A pack, a powder, a suspension, a spray or a serum, and the like.

In addition, the cosmetic composition of the present invention may contain customary adjuvants such as stabilizers, solubilizers, vitamins, pigments, and flavorings commonly used in the field of the composition. In the composition, the content of the rhubarb extract or fraction thereof may be an amount effective to achieve the preventive or ameliorative effect of acne, for example, 0.001 to 10% by weight based on the total weight of the composition, preferably about 0.01 To 5% by weight.

The present invention also provides a food composition for preventing or ameliorating acne, comprising a rhubarb extract or a fraction thereof as an active ingredient.

When the rhubarb extract or fraction thereof of the present invention is used as a food additive, the rhubarb extract or its fraction may be used as it is or may be used together with other food or food ingredients, and may be suitably used according to a conventional method. The amount of the active ingredient to be mixed can be suitably determined according to the intended use (prevention, health or therapeutic treatment). Generally, the rhubarb extract or fraction thereof of the present invention is added in an amount of not more than 15% by weight, preferably not more than 10% by weight based on the raw material. However, in the case of long-term intake for the purpose of health and hygiene or for the purpose of controlling health, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range.

There is no particular limitation on the kind of the food. Examples of the foods to which the above substances can be added include dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen noodles, gums, ice cream, various soups, drinks, tea, Alcoholic beverages, and vitamin complexes, and includes foods in a conventional sense.

In addition to the above, the composition of the present invention may further contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, A carbonating agent used in a carbonated beverage, and the like. In addition, the composition of the present invention may comprise flesh for the production of natural fruit juices, fruit juice drinks and vegetable drinks. These components may be used independently or in combination. Although the ratio of such additives is not critical, it is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

Hereinafter, preferred embodiments and production examples are shown to facilitate understanding of the present invention. However, the following Examples and Preparation Examples are provided only for the purpose of easier understanding of the present invention, and the present invention is not limited by the Examples and Production Examples.

Example 1. Preparation of rhubarb extract and its fractions

The rhubarb used in this experiment was purchased from Ulleungsan in late September 2010 and used for the experiment. The voucher specimen is housed in Pukyong National University Food Engineering and Food Microbiology classrooms. First, the rhubarb was washed with water to remove the salt, dried, and powdered with a blender (HMF-1000A; Hanil Electronics, Seoul, Korea) and stored at -20 ° C until use. Then 1.0 L of methanol was added to 10 kg of rhubarb powder, and the mixture was stirred at 70 캜 for 3 hours, filtered and concentrated under reduced pressure with a rotary evaporator (Eyela, Tokyo, Japan) to obtain a methanol extract. Water was added to the methanol extract to make 1 L of a 10% methanol solution, and then n-hexane, dichloromethane, ethyl acetate and n-butanol were sequentially added to obtain fractions. The extracts and fractions were dissolved in dimethyl sulfoxide ), The concentration was adjusted to 200 mg mL < ^{-1 &} gt ;, and stored at -70 DEG C until use.

Example 2. Microorganisms and culture

All of the strains used in the experiment were purchased from KCM Korea Center for Microorganism Conservation (KCCM). Staphylococcus aureus (KCTC 1927), Staphylococcus epidermidis (KCTC 1370), and Pseudomonas aeruginosa (KCTC 1637) were used as the strains used in the experiment. The strains used were Propionibacterium acnes (KCTC 3314), Staphylococcus aureus Respectively.

Propionibacterium acnes strains were anaerobically cultured in brain heart infusion broth (BHI; Difco Inc., Detroit, MI, USA) supplemented with 1.0% glucose and incubated in a CO ₂ incubator (NAPCO 5400 ; General Laboratory Supply, Pasadena, TX, USA).

Staphylococcus aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa were aerobically cultured in TSB (Tryptic soy broth) at 37 ° C.

Example 3 Isolation and Purification of Florotannine from Rhubarb

Compounds related to antimicrobial activity were isolated from EtOAC fractions of rhubarb extracts which showed the strongest antimicrobial activity. More specifically, six compounds were obtained from successive chromatographies using the MeOH as a solvent from the rhodium EtOAC fractions, and as a result, it was confirmed that the compounds of the formula (1), fucofuroxole-A (2) (3), dioxinodihydrocoke (4), flurofucofurocon-A (5) and dihydroxy (6).

Experimental Example 1. Confirmation of antimicrobial activity of the rhubarb extract and its fractions

Disc diffusion assay was used to confirm the antimicrobial activity. Bacteria were cultured in TSB medium at 600 nm until absorbance at 600 nm, and 100 microliter culture containing about 10 ⁴ to 10 ⁵ CFU / mL of the culture was spread on MHA (Mueller-Hinton agar) plate . Thereafter, the culture was incubated at 37 ° C for 24 hours and the diameter of the inhibition zone was measured to confirm the antimicrobial activity of the methanol extract of the rhubarb and the fraction thereof against Propionibacterium acnes. The results are shown in Table 1 below.

Strain
density Retaining ring (mm) MeOH Hexane DCM EtOAc BuOH H ₂ O Staphylococcus aureus
(Staphylococcus aureus)
KCTC 1927 1 mg disc ^-1 7.0 8.0 8.0 10.0 9.0 - 5 mg disc ^-1 14.0 9.0 10.0 16.0 11.0 - Staphylococcus epidermidis
(Staphylococcus epidermidis)
KCTC 1370 1 mg disc ^-1 7.0 7.0 7.0 10.0 7.0 - 5 mg disc ^-1 14.0 11.0 12.0 20.0 12.0 - P. aeruginosa
(Pseudomonas aeruginosa)
KCTC 1637 1 mg disc ^-1 - - - - - - 5 mg disc ^-1 - - - 7.5 - - Propionibacterium acnes
(Propionibacterium acnes)
KCTC 3314 1 mg disc ^-1 - 9.0 7.0 9.0 - - 5 mg disc ^-1 8.0 10.0 7.0 12.0 - - P. acnes segregation
2875 1 mg disc ^-1 6.5 7.0 7.0 9.0 8.0 - 5 mg disc ^-1 11.0 11.0 10.0 14.0 9.0 - P. acnes segregation
2876 1 mg disc ^-1 - 8.0 6.5 9.0 - - 5 mg disc ^-1 10.0 11.0 9.0 19.0 8.0 -

As shown in Table 1, the Daehang MeOH extract showed antimicrobial activity against acne bacterium, especially when treated with the rhubarb EtOAc fraction. However, the above extracts were less effective than the antimicrobial activity against Gram-positive bacteria, which are antimicrobial activities against Gram-negative bacteria, and did not exhibit antimicrobial activity against all bacteria.

Experimental Example 2. MIC and MBC measurement of the rhubarb extract and its fractions

The minimum inhibitory concentration (MIC) is the lowest concentration that inhibits the visible growth of microorganisms after overnight incubation and is defined as the minimum inhibitory concentration at which the bacteria do not grow well. MBC (minimun bactericidal concentration) is defined as 99.99% The minimum concentration is defined as the minimum sterilization concentration at which no bacteria grows. The two-fold serial dilution method using Mueller-Hinton medium was used for the MIC and MBC measurements of the rhubarb methanol extract and sequential fractions, and the results are shown in Table 2.

Strain
MeOH Hexane DCM EtOAc BuOH H ₂ O MIC MBC MIC MBC MIC MBC MIC MBC MIC MBC MIC MBC P. acnes
KCTC 3314 1024 1024 256 256 512 512 128 128 512 512 P.acnes 2875 512 1024 256 512 512 1024 128 256 1024 > 1024 - - P.acnes 2876 512 1024 256 512 512 1024 256 512 1024 > 1024 - - P. aeruginosa
KCTC 1637 > 1024 > 1024 > 1024 > 1024 > 1024 > 1024 > 1024 > 1024 > 1024 > 1024 - - S. aureus
KCTC 1927 1024 2048 256 1024 256 2045 128 512 512 2048 - - S. epidermidis
KCTC 1370 > 1024 > 1024 256 > 1024 512 1024 128 512 1024 > 1024 - -

As shown in Table 2, the MIC and MBC values of the fractions against Propionibacterium acnes varied according to the polarity of the solvent, and it was confirmed that the fraction of the rhubarbital EtOAc showed the lowest value. This indicates that the rhubarb EtOAc fraction has the highest antimicrobial activity.

On the other hand, the rhubarb extract and its fractions showed no antimicrobial activity against P. aeruginosa, and P. aeruginosa showed the highest MIC value in all fractions compared to other Gram - positive bacteria.

Further, in order to confirm the MIC of phlorotannin and antibiotic acne bacteria isolated from the rhubarb fraction at the time of Example 3, tetracycline, erythromycin and lincomycin, which are antibiotics used in the treatment of acne, The MIC for ACKNE was measured and the results are shown in Table 3.

Strain
MIC (ml ml ^-1 ) C1
(EK) C2
(FF) C3
(7P) C4
(DD) C5
(PFF) C6
(DE) Tetracycline Erythromycin Linco
Mitine P. acnes
KCTC 3314 256 32 128 64 256 256 64 2048 1024 P.acnes 2875 256 32 256 128 256 256 64 2048 1024 P.acnes 2876 256 64 256 128 256 256 64 2048 1024 S. aureus
KCTC 1927 256 128 256 128 256 256 0.5 2 8 S. epidermidis
KCTC 1370 128 64 128 64 128 128 2 0.125 0.25

As shown in Table 3, the MIC value of Florotannine for Propionibacterium acnes is 32-64 g ml ^-1 , which is at least double that of tetracycline, erythromycin and lincomycin MIC. In addition, it was confirmed that fucofuroform-A (FF) exhibited the highest antimicrobial activity among the fluorotannine compounds.

Experimental Example 3. Confirmation of Synergy Effect of Rhubarb Extract and Antibiotic against Acne

In order to confirm the interaction between the rhubarb extract and the antibiotic against the acne bacterium, it was confirmed that fucofuroxole-A (FF), an active ingredient of the rhubarb extract extracted through Experimental Example 2, and tetracycline, an antibiotic used in acne treatment, MIC and FIC (fractional inhibitory concentration) of Propionibacterium acnes were measured using the checkerboard method after combination of mycine and lincomycin. The results are shown in Table 4. The FIC is calculated as the MIC used as an antibiotic or PFF alone, and the FIC index shows synergy when:

synergism <1, indifference ≥ 1 to ≤ 2, antagonism> 2

_{FIC A = MIC A in combination /} MIC A

FIC _B = MIC _B in combination / MIC _B

FIC Index = FIC _A + FIC _B

Strain

Tetracycline
Erythromycin Lincomycin MIC FIC index MIC FIC index MIC FIC index A B C b c A B C b c A B C b c P. acnes
KCTC 3314 64 32 16 1,000 0.750 2048 64 8 0.531 0.504 1024 256 2 0.750 0.502 P.acnes 2875 64 16 8 0.750 0.625 2048 32 16 0.516 0.508 1024 32 16 0.531 0.516 P.acnes 2876 64 32 16 1,000 0.750 2048 64 One 0.531 0.500 1024 4 2 0.504 0.502

A: Fucopurecoccol-A none; B and b: fucofurocon-A at 32.0 g ml ^-1 ; C and c: fucoprochol-A at 64.0 ㎍ ml ^-1 .

FIC index means synergism ≤ 0.5, indifference> 0.5 to ≤ 4, antagonism> 4.

As shown in Table 4, the MIC value of tetracycline was reduced from 16 to 8 when injected with FF of 64 ig ml ^-1 , and erythromycin and lincomycin also decreased when injected with FF. Since the FIC index of the antibiotics injected with FF is between 0.500 and 0.750, this indicates the interaction of FF with antibiotics, and the effect of fucofuroxole-A (FF), an active ingredient of rhubarb extract, and antibiotics, &Lt; / TABLE &gt;

Hereinafter, formulation examples of the composition of the present invention will be described, but the present invention is not intended to be limited thereto but is specifically described.

Formulation Example 1. Pharmaceutical preparation

1-1. Manufacture of Powder

20 mg of rhubarb extract or its fractions

Lactose 100 mg

Talc 10 mg

The above components are mixed and filled in airtight bags to prepare powders.

1-2. Manufacture of tablets

10 mg of the rhubarb extract or its fractions

Corn starch 100 mg

Lactose 100 mg

Magnesium stearate 2 mg

After mixing the above components, tablets are prepared by tableting according to the usual preparation method of tablets.

1-3. Preparation of capsules

10 mg of the rhubarb extract or its fractions

Crystalline cellulose 3 mg

Lactose 14.8 mg

Magnesium stearate 0.2 mg

The above components are mixed according to a conventional capsule preparation method and filled in gelatin capsules to prepare capsules.

1-4. Injection preparation

10 mg of the rhubarb extract or its fractions

180 mg mannitol

Sterile sterilized water for injection 2974 mg

Na ₂ HPO ₄ .2H ₂ O 26 mg

(2 ml) per 1 ampoule in accordance with the usual injection preparation method.

1-5. Manufacture of liquid agent

20 mg of the rhubarb extract or its fractions

10 g per isomer

5 g mannitol

Purified water quantity

Each component was added and dissolved in purified water according to the usual liquid preparation method, and the lemon flavor was added in an appropriate amount. Then, the above components were mixed, and purified water was added thereto. The whole was added with purified water to adjust the total volume to 100 ml, And sterilized to prepare a liquid preparation.

Formulation Example 2. Cosmetic preparation

2-1. Manufacture of softening longevity

10 mg of the rhubarb extract or its fractions

Glycerin 30 g

20 g of butylene glycol

Propylene glycol 20 g

10 g of carboxyvinyl polymer

100 g ethanol

Triethanolamine 10 g

Trace amount of perfume

Fine amount of purified water

2-2. Manufacture of nutrition cream

10 mg of the rhubarb extract or its fractions

100 g of wax

Polysorbate 60 15 g

50 g of sorbitan sesquioleate

100 g of liquid paraffin

Squalane 50 g

Caprylic / capric triglyceride 50 g

20 g of triethanolamine

Trace amount of perfume

Fine amount of purified water

Formulation Example 3. Food preparation

3-1. Manufacture of health food

Ruminant extract or its fractions 100 mg

Vitamin mixture quantity

70 g of vitamin A acetate

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

0.15 mg of vitamin B2

Vitamin B6 0.5 mg

0.2 g of vitamin B12

Vitamin C 10 mg

Biotin 10 g

Nicotinic acid amide 1.7 mg

Folate 50 g

Calcium pantothenate 0.5 mg

Mineral mixture quantity

1.75 mg of ferrous sulfate

0.82 mg of zinc oxide

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Secondary calcium phosphate 55 mg

Potassium citrate 90 mg

Calcium carbonate 100 mg

Magnesium chloride 24.8 mg

3-2. Manufacture of health drinks

Ruminant extract or its fractions 100 mg

Vitamin C 15 g

Vitamin E (powder) 100 g

19.75 g of ferrous lactate

Oxide 3.5 g

Nicotinic acid amide 3.5 g

Vitamin A 0.2 g

Vitamin B1 0.25 g

Vitamin B2 0.3g

Water quantification

Although the compositional ratio is relatively mixed with a component suitable for a favorite drink, it is also possible to arbitrarily modify the compounding ratio according to the regional or national preference such as the demand class, the demanding country, and the use purpose.

Claims (11)

A pharmaceutical composition for preventing or treating acne, comprising fucofuroeckol-A (FF) as an active ingredient. The pharmaceutical composition for preventing or treating acne according to claim 1, wherein the fucofuroeckol-A (FF) is isolated from a rhubarb extract or a fraction thereof. The pharmaceutical composition for preventing or treating acne according to claim 2, wherein the rhubarb extract is obtained by extracting with methanol. The pharmaceutical composition according to claim 2, wherein the rhubarb fraction is obtained by fractionating the rhubarb extract with ethyl acetate. delete The pharmaceutical composition for preventing or treating acne according to claim 1, wherein the composition has an antibacterial activity against acne bacteria. The pharmaceutical composition for preventing or treating acne according to claim 6, wherein the acne bacterium is Propionibacterium acnes. The use according to claim 1, wherein the composition further comprises one or more antibiotics selected from the group consisting of tetracycline, erythromycin, and lincomycin. A pharmaceutical composition for therapeutic use. A cosmetic composition for prevention or improvement of acne, comprising fucofuroeckol-A (FF) as an active ingredient. The cosmetic composition according to claim 9, wherein the composition has antimicrobial activity against acne bacteria. A food composition for prevention or improvement of acne, comprising fucofuroeckol-A (FF) as an active ingredient.