KR101669501B1

KR101669501B1 - Cell harvesting apparatus

Info

Publication number: KR101669501B1
Application number: KR1020150120971A
Authority: KR
Inventors: 김태훈; 박한호; 이진호; 김정부
Original assignee: 주식회사 지디이
Priority date: 2015-08-27
Filing date: 2015-08-27
Publication date: 2016-10-26

Abstract

The present invention relates to a device for concentrating and harvesting a microalgae biomass. The device comprises: a cylindrical body having a longitudinally penetrated internal spatial part and a lower side drainage hole connected thereto; a circular filter disc horizontally disposed in the upper side of the drainage hole in the spatial part of the body; a cap coupled with the upper side of the body, and having a cell feeding hole formed in one side thereof; a vibration generating unit disposed in the lower side of the body, and longitudinally vibrating the filter disc; and a waterproof film interposed between the body and the vibration generating unit, and blocking the lower side of the spatial part of the body. Cells are collected to the lower side of the filter disc from cell culturing water supplied through a feeding hole of the cap by means of vibration of the filter disc caused by the vibration generating unit. Water is discharged to the outside through the drainage hole of the body after passing through the filter disc.

Description

[0001] CELL HARVESTING APPARATUS [0002]

TECHNICAL FIELD The present invention relates to a microalgae biomass concentrating and harvesting apparatus, and more particularly, to a microalgae biomass concentrating harvesting apparatus capable of enhancing cell collection performance by vibrating a filter disk up and down using a low frequency oscillating loudspeaker to be.

As a technique relating to an extraction device such as a microorganism,

Korean Patent Registration No. 10-1430727 (Registered as "Prior Art" hereinafter), "Classification and Extraction Apparatus for Microparticles or Microorganisms and Classification and Extraction Method Using the Microparticles"

The present invention relates to a microparticle or microbial sorting and extracting apparatus and a sorting and extracting method using the microparticle or microbial sorting and extracting apparatus. The microbial sorting and extracting apparatus includes a chamber block A step of adhering a slide plate to the chamber block; a step of supplying a fluid containing microparticles or microorganisms of different sizes inside the chamber block through an inlet formed in the chamber block; Applying a pressure to slide the microparticles or microorganisms along the slide plate to sort the microparticles or microorganisms into the respective chamber spaces according to sizes; and after sucking one surface of the chamber block into the suction means, The chamber space is opened one by one, From isolated by a technique comprising the step of: extracting each of the classification the fine particles or microorganisms size.

The present invention relates to a method for enhancing cell collection performance on the upper surface of a filter disk by vertically and repeatedly vibrating a filter disk by a low frequency repetition frequency using a low frequency oscillating loudspeaker and collecting the collected cells by collecting the filter disk and storing the collected cells in a separate container And to provide a microalgae biomass concentrating and harvesting device capable of producing a biomass.

According to an aspect of the present invention, there is provided an apparatus for concentrating and harvesting microalgae biomass,

A cylindrical body having upper and lower through-type inner space portions and lower side drain ports communicating with the upper and lower through-holes;

A circular filter disk disposed horizontally above the drain hole in the space of the body;

A cap coupled to the top of the body and having a cell inlet on one side;

A vibration generating unit disposed below the body for vibrating the filter disk up and down; And

A waterproof membrane interposed between the body and the vibration generating unit to block a space under the space of the body;

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The cells are collected on the filter disk by the vibration of the filter disk by the vibration generating unit in the number of cell cultures supplied through the inlet of the cap and the water is drained to the outside through the filter disk and through the drain of the body .

The microalgae biomass concentrating and harvesting apparatus according to the present invention comprises:

Instead of the high frequencies that can affect the cells and damage them, the cells can be smoothly collected on the upper surface of the filter disk, which is oscillated up and down by repeated oscillations using a low frequency. At the same time, It is possible to drain cells to the drain port side through the disk, thereby greatly improving the cell collection performance and minimizing the damage to the cells.

1 is a cross-sectional view showing a microalgae biomass concentration and harvesting apparatus according to the present invention,
Fig. 2 is a sectional view of the separated state of Fig. 1; Fig.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

Prior to this, terms and words used in the present specification and claims should not be construed as limited to ordinary or dictionary terms, and the inventor should appropriately interpret the concepts of the terms appropriately It should be interpreted in accordance with the meaning and concept consistent with the technical idea of the present invention based on the principle that it can be defined. Therefore, the embodiments described in the present specification and the configurations shown in the drawings are merely the most preferred embodiments of the present invention and are not intended to represent all of the technical ideas of the present invention. Therefore, various equivalents It should be understood that water and variations may be present.

1 and 2, the microalgae biomass concentration and harvesting apparatus according to the present invention comprises:

A filter disk 20, a cap 30, a vibration generating unit 40, and a waterproofing film 50. The body 10, the filter disk 20, the cap 30,

Looking at each configuration,

The body 10

As shown in Figs. 1 and 2,

A vertical penetrating inner space portion 11, and

A drain hole 12 communicating with the space portion 11 and disposed on the lower side,

As shown in Fig.

At this time, the body 10 is composed of first and second powders 10A and 10B which are divided into upper and lower halves,

The powders 10A and 10B can be joined and separated by a fastening means 10C.

The fastening means 10C includes a first flange 15 formed along the lower periphery of the first powder 10A and a second flange 15 formed along the upper periphery of the second powder 10B, A bolt 17 radially fitted to the first and second flanges 15 and 16 and a nut 18 fastened to the first and second flanges 15 and 16.

As a result, each of the powders 10A and 10B can be mutually coupled and separated by the fastening means 10C, and in particular, the filter disk 20 can be replaced in a separated state.

The filter disc 20

As shown in Figs. 1 and 2,

(Hereinafter referred to as "cell") supplied to the space 11 of the body 10,

And a circular filter structure disposed horizontally above the drain port (12) in the space (11) of the body (10).

The filter disc 20 is preferably a filter structure having a pore size such that cells are collected and water can pass through.

The filter disc 20 is fixedly supported by the respective powders 10A and 10B which are mutually coupled with each other in a state interposed in a region between the powders 10A and 10B.

Therefore, as described above, it is possible to replace the filter disc 20 in a state where the respective powders 10A and 10B are separated from each other.

The cap 30

As shown in Figs. 1 and 2,

Which is coupled to the upper portion of the body 10,

On one side, a cell inlet (31)

.

At this time, a screw thread is formed on the inner circumferential surface of the cap 30 and a corresponding screw thread is formed on the outer circumferential surface of the body 10, so that the cap 30 is fastened to the body 10 ).

The cap 30 further includes an air pressure relief hole 32 for the space portion 11 of the body 10.

As a result, the pneumatic pressure relief hole 32 is formed in such a manner that the culture water accommodated in the space portion 11 of the body 10 is drained through the drain port 12 of the body 10 or the inlet port 31 of the cap 30 It is possible to relieve the air pressure in the space portion 11 of the body 10 when the culture water is introduced and supplied to the space portion 11 of the body 10. [

The air pressure relief hole 32 may further be provided on the side of the second powder 10B of the body 10.

The vibration generating unit 40

As shown in Figs. 1 and 2,

And is disposed below the body 10 to vibrate the filter disk 20 up and down,

A rectangular box-shaped main body 41,

A low frequency oscillation speaker 42 disposed at the center of the upper portion of the main body 41,

A volume control unit 43 provided on the front surface of the main body 41 for controlling the speaker 42,

.

The vibration generating unit 40 further includes a sub cap 44 provided on the main body 41 to surround the speaker 42. The sub cap 44 has a thread on the inner peripheral surface thereof A corresponding thread is formed on the lower outer peripheral surface of the second powder 10B of the body 10 so that the sub cap 44 is coupled to the body 10 as the thread and the corresponding thread are fastened to each other.

The waterproof membrane (50)

As shown in Figure 1,

The vibration generating unit 40 is interposed between the body 10 and the vibration generating unit 40 to block the space 11 of the body 10,

And a circular thin plate structure of an elastic material which is closely attached to the body 10 side by the subcap 44 of the vibration generating unit 40 while surrounding the lower periphery of the body 10.

Meanwhile, the microalgae biomass concentration and harvesting apparatus according to the present invention

As shown in Figure 1,

A water level sensor 60 provided on the other side of the cap 30 for storing the culture water contained in the space 11 of the body 10,

A supply line 70 connected to the inlet 31 of the cap 30 for interconnecting the inlet 31 and the culture tank 1,

A pump 80 disposed on the supply line 70,

A controller 90 for controlling the operation of the pump 80 according to a sensed value of the level sensor 60,

.

[0030] In operation of the microalgae biomass concentration and harvesting apparatus according to the present invention having the above-described structure,

The operation of the pump 80 causes one end of the culture water to be introduced into the supply line 70 located in the culture tank 1 and supplied to the space portion 11 of the body 10,

The controller (90) receiving the sensed value of the water level sensor (60) compares the sensed value with a preset value to limit the operation of the pump (80)

The filter disk 20 located in the space portion 11 of the body 10 in which the supply of the culture water is stopped is rotated at a predetermined interval by a low frequency oscillated by the speaker 42 of the vibration generating unit 40 Repeatedly,

At this time, the cells are collected on the upper surface of the filter disk 20 which vibrates up and down,

Water that has passed through the filter disc 20 is drained through the drain port 12 when the drain port 12 of the body 10 is opened,

When a certain amount of cells are collected by repeating the above process, each powder 10A, 10B of the body 10 is separated and the filter disk 20 is taken out, and the collected cells are collected and stored in a separate container or the like.

While the present invention has been described with reference to the accompanying drawings, it is to be understood that the invention has been described with reference to the "apparatus for concentrating and extracting microalgae biomass" having a specific shape and structure. However, the present invention can be variously modified and changed by those skilled in the art, Modifications are to be construed as falling within the scope of protection of the present invention.

10: Body
10A: First powder
10B: Second powder
11: Space portion
12: Sewer
10C: fastening means
15: 1st flange
16: second flange
17: Bolt
18: Nuts
20: Filter disk
30: Cap
31: Inlet
32: Air pressure relief hole
40: vibration generating unit
41:
42: Speaker
43: Regulator
44: Subcap
50: Waterproof membrane
60: Level sensor
70: Supply line
80: Pump
90: Controller

Claims

A cylindrical body 10 having an upper and a lower penetrating inner space 11 and a lower side drain 12 communicating therewith;
A circular filter disk 20 horizontally disposed above the drain hole 12 in the space 11 of the body 10;
A cap 30 coupled to the upper portion of the body 10 and having a cell inlet 31 at one side thereof;
A vibration generating unit (40) disposed under the body (10) for vibrating the filter disk (20) up and down; And
And a waterproofing film 50 interposed between the body 10 and the vibration generating unit 40 to block a space below the space 11 of the body 10,

The vibration generating unit (40)
A box-shaped main body 41;
A low frequency oscillation speaker 42 disposed at the center of the main body 41;
A volume control 43 for the speaker 42 provided on the front surface of the main body 41; And
And a subcap (44) provided on the main body (41) to surround the speaker (42)

The vibration generating unit 40 and the body 10 are connected to each other by a screw thread formed on the inner circumferential surface of the sub cap 44 and a screw thread formed on a lower outer circumferential surface of the body 10, Wherein the microbial biomass concentrate harvesting device is combined with the microalgae biomass concentrate harvesting device.

The method according to claim 1,
A water level sensor 60 provided on the other side of the cap 30 for storing the culture water contained in the space 11 of the body 10,
A supply line 70 connected to the inlet 31 of the cap 30,
A pump 80 disposed on the supply line 70,
A controller 90 for controlling the operation of the pump 80 according to a sensed value of the level sensor 60,
Further comprising a microbial biomass concentrating and harvesting device.

The method according to claim 1,
Characterized in that the cap (30) is provided with an air pressure relief hole (32) for the space (11) of the body (10).

4. The method according to any one of claims 1 to 3,
The body 10 is composed of first and second powders 10A and 10B which are divided in half by half,
Each of the powders 10A and 10B can be joined and separated by a fastening means 10C,
Wherein the filter disc (20) is fixedly supported by the respective powders (10A, 10B) interposed and interposed between the respective powders (10A, 10B).

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