KR101650119B1 - Scaffold manufacturing device using UV hardening - Google Patents

Abstract

The present invention relates to a scaffold manufacturing apparatus using UV curing, and an object of the present invention is to provide a scaffold manufacturing apparatus using a UV curing method, Thereby enabling a quick curing to be carried out, thereby improving the productivity. The present invention also provides an apparatus for manufacturing a scaffold using UV curing.

Description

[0001] The present invention relates to a scaffold manufacturing device using UV hardening,

The present invention relates to an apparatus for manufacturing a scaffold using UV curing, and more particularly, to a scaffold manufacturing apparatus using UV curing, And more particularly, to a scaffold manufacturing apparatus using UV curing which enables rapid curing to be performed to improve productivity.

Tissue engineering is a technology that is based on the basic concepts and technologies of life sciences, medicine, and engineering to create a substitute for living tissue and transplant it into the living body to make it possible to maintain, improve and restore the function of the living body. will be. As artificial skin was produced for the first time in the 1980s, it began to be recognized as a new field of study and various studies have been made to date. In the case of organs, which are very complex tissues, they have not been far apart from the research stage yet, but they have been developed to be widely used for relatively simple tissues such as skin and bones.

The practical implementation of biotissue engineering is to collect the necessary tissues from the body of the patient, separate the cells from the tissue, and then proliferate the separated cells by the required amount through cultivation, plant them in a porous biodegradable polymer scaffold, (Scaffold) is formed by implanting the cell culture supporter (scaffold) formed in the body into the body again. After transplantation, most tissues and organs are fed with oxygen and nutrients by diffusion of body fluids until new blood vessels are formed. When blood vessels enter the body and blood is supplied, cells multiply and differentiate into new tissues and organs And the biodegradable polymer scaffold has disintegrated and disappeared in the meantime.

The main requirements of the material of the support used for regeneration of the human tissue are as follows. First of all, it is essential that the tissue cells should be well adhered thereon, and that the tissue cells have a mechanical strength enough to function as a substrate or support so as to form a tissue having a three-dimensional structure by adhering to the surface of the material do. It should also act as an intermediate barrier between the transplanted cells and host cells, which requires a non-toxic biocompatibility that does not result in blood clotting or inflammation after transplantation. In addition, when the transplanted cells function as a new body tissue, they must have biodegradability that can be completely decomposed and disappeared in a desired period of time.

There have been various studies related to scaffold fabrication techniques to satisfy various conditions as described above. Classical methods include solvent-casting particulate leaching, salt foaming, fiber meshes / fiber bonding, phase separation, melt molding, , Freeze drying, and the like have been used to make scaffolds. However, as the demand for scaffolds that need to mimic some very complex physical shapes of a human body, such as ear cart carts, is increasing, there is a need for scaffolding that can produce more sophisticated and complex shapes than these classical methods Research on devices is becoming more and more active.

Solid freeform fabrication (SFF) has been introduced as a technique for producing a scaffold having such complex and elaborate three-dimensional shapes. The SFF (Solid Freeform Fabrication) has the advantage of being able to freely create a desired three-dimensional shape so as to be able to realize a fairly complicated shape such as a pinna shape and the like, as well as the pore size of the scaffold ), Porosity and inter-pore interconnection, allowing the cells to penetrate into the scaffold more easily and to increase nutrient circulation and oxygen supply. Representative examples include SLA (Stereo Lithography Apparatus), SLS (Selective Laser Sintering), FDM (Fused Deposition Modeling), 3D printing, and 3D plotting.

Among them, 3D Plotting is a technique of melting a polymer suitable for living tissue and pushing it through a nozzle with pneumatic pressure to produce a three-dimensional scaffold. The cylinder head equipped with the nozzle moves freely in the XYZ direction And the molten polymer is allowed to cure instantly as it passes through the nozzle and reaches the bottom or scaffold surface, thereby making it possible to freely produce a three-dimensional shape. There are many advantages such as the possibility of forming in the solution or in the air by using the 3D floating. Korean Patent Publication No. 2010-0072326 filed by the present applicant entitled " Method of manufacturing a cell culture support "(Feb. 26, 2012) describes a three-dimensional scaffold fabrication technique based on such a three-dimensional floating technique.

The 3D floating method has the advantage that it is possible to produce very complicated and sophisticated shapes, but it also increases the risk of inaccuracies and defects in the manufacturing process due to the complicated shapes. The three-dimensional floating technique forms the shape by pushing the molten polymer solution to the nozzle by curing, as described above. At this time, if another material is accumulated in the material which is not completely cured yet, Problems can arise. Therefore, in the three-dimensional floating apparatus, it takes time to cure the molten polymer solution sufficiently to prevent the shape of the molten polymer solution from being discharged out of the nozzle, so that there is a problem in improving the scaffold production efficiency.

1. Korean Patent Publication No. 2010-0072326 "Method for manufacturing cell culture support" (2012.02.06)

SUMMARY OF THE INVENTION Accordingly, it is an object of the present invention to provide a polymer hydrogel solution which is a material of a scaffold in the process of producing a three-dimensional scaffold, The present invention provides an apparatus for manufacturing a scaffold using UV curing which enables rapid curing by UV at the time of jetting, thereby improving productivity.

In order to accomplish the above object, the apparatus for manufacturing a scaffold using UV curing according to the present invention comprises a solution storage part 110 for storing a solution, a nozzle part for spraying a solution supplied from the solution storage part 110 120), a heating means (130) for controlling the temperature of the solution stored in the solution storage portion (110); A collecting part 200 formed in a cylindrical shape and provided at a lower part of the injection device 100 to collect a solution injected through the nozzle part 120 of the injection device 100; A jet position adjusting unit 300 for adjusting the jet position by moving the jetting unit 100 or the collecting unit 200 in X, Y, and Z directions; A UV irradiation unit 400 formed in a ring shape and provided around the nozzle unit 120 for irradiating UV light onto a solution sprayed and discharged from the nozzle unit 120; . ≪ / RTI >

The UV irradiating unit 400 includes a ring frame 410 formed in a ring shape and provided around the solution discharge hole of the nozzle unit 120, The UV light source 450 is formed of an optical fiber and has one end connected to the light irradiation hole 420 and the other end connected to the UV light source 450, And a plurality of optical path forming parts 430 that form an optical path such that the UV light emitted from the light guide hole 420 is irradiated through the light reflecting hole 420.

The UV irradiating unit 400 is formed such that the UV light beams irradiated from the plurality of the light irradiation holes 420 are collected at a point on the central axis of the ring frame 410, 120) discharge position.

The optical projection hole 420 is formed such that the end of the optical path forming portion 430 faces the central axis of the ring frame 410 so that the cross- And may be formed in a direction inclined at an acute angle with respect to the axial direction.

The UV irradiation unit 400 may be formed in a pipe shape and provided inside the ring frame 410 to guide the direction of the optical path forming unit 430 by inserting the optical path forming unit 430 And an optical path guide unit 440. [ The optical path guide part 440 is disposed so that an extension direction of the optical path guide part 440 is perpendicular to the central axis of the ring frame 410 so that the end of the optical path forming part 430 faces the center axis of the ring frame 410, May be formed in a direction inclined at an acute angle with respect to the central axis direction. Or the optical path guide part 440 is hinged to the ring frame 410 so as to adjust the angle between the extending direction of the optical path guide part 440 and the central axis direction of the ring frame 410 .

The solution may be a mixture of a hydrogel and a UV linker which is cured by UV. In this case, the hydrogel may be selected from the group consisting of PEG (polyethylene glycol), collagen, albumin, poly (amino acid), cellulose, agarose, alginate, heparin heparin, hyaluronic acid, chitosan, and the like.

According to the present invention, in a scaffold made of a three-dimensional floating technique, there is a great effect that quick curing is performed by effectively irradiating UV at the time of spraying a polymer hydrogel solution which is a material of a scaffold. Thus, the productivity can be improved by solving the problem of lowering the production efficiency, which has been caused by the time required for curing the polymer solution in the conventional case.

In particular, according to the present invention, it is possible to irradiate UV at a precise position where the polymer solution is discharged from the nozzle portion of the three-dimensional scaffold fabricating apparatus, so that compared to simply irradiating the polymer solution at a fixed position such as under the substrate It is possible to perform sophisticated control and to control the UV irradiation according to the solution discharge position from the outside, there is a great advantage that the problem that the configuration becomes excessively complicated can be removed originally. Accordingly, there is an economical advantage that the production cost of the scaffold manufacturing apparatus is not significantly increased as compared with the conventional apparatus while the UV irradiation structure is provided.

1 is a perspective view of a three-dimensional scaffold manufacturing apparatus.
2 is a cross-sectional view of a nozzle portion and a UV irradiating portion.
3 is a detailed view of a UV irradiation part.
4 is an enlarged sectional view of a part of the UV irradiation part.
5 shows a problem of the conventional UV irradiation method.

Hereinafter, an apparatus for manufacturing a scaffold using UV curing according to the present invention will be described in detail with reference to the accompanying drawings.

1 is a perspective view of a three-dimensional scaffold manufacturing apparatus. As shown in FIG. 1, the apparatus 1000 for manufacturing scaffolds according to the present invention mainly includes a spraying unit 100, a collecting unit 200, a spraying position adjusting unit 300, and a UV irradiating unit 400.

The injection means 100 includes a solution storage portion 110 in which the biocompatible material is dissolved or melted and stored therein, a nozzle portion 120 through which the solution supplied from the solution storage portion 110 is injected, And a heating means 130 for controlling the temperature of the solution stored in the heating means. The solution storage part 110 is heated by the heating unit 130 so that the solution stored therein can be dissolved or melted.

In the present invention, UV irradiation is performed so that the solution can be cured more quickly after the solution is discharged, as will be described in detail later. Generally, the biocompatible material used as a scaffold material is made of a biodegradable polymer material, but such materials alone do not cure by UV irradiation. Therefore, in order to facilitate curing by UV irradiation, the solution is composed of a mixture of a hydrogel and a UV linker having a property of being cured by UV. Since the curing of the solution by the UV irradiation is substantially caused by the UV linker, the hydrogel generally can be applied to various materials used as a material of the scaffold. Specifically, the hydrogel may be selected from the group consisting of PEG (polyethylene glycol), collagen, albumin, poly (amino acid), cellulose, agarose, alginate, , Heparin, hyaluronic acid, chitosan, and the like.

The collecting unit 200 is disposed below the injecting unit 100 to collect the solution injected through the nozzle unit 120 of the injecting unit 100. At this time, the collecting unit 200 may be formed into a flat plate shape according to the shape of the scaffold to be produced, or may be formed into a specific shape such as a conduit shape or the like (in order to make a scaffold used for blood vessels, etc.) have.

The injection position adjuster 300 adjusts the injection position by moving the injection device 100 in the X, Y, and Z directions. In the embodiment of FIG. 1, the injection position adjustment unit 300 includes a fixing bracket 310 having Z-axis guide rails 320 for moving the injection means 100 in the Z-axis direction, respectively. The X-axis guide rail 330 and the Y-axis guide rail 340 move the fixing bracket 310 in the X-axis and the Y-axis, respectively, . For example, the injection position may be adjusted by moving the collecting unit 200 in the X, Y, and Z directions, and the injection unit 100 and the collecting unit 200 may be controlled, The ejection position regulating unit 300 may have any other shape as long as it can move the relative position of the ejection position regulating unit 300 with a degree of freedom in the three axial directions. The apparatus 1000 for manufacturing scaffolds according to the present invention may include a control unit 500 connected to the injection position adjusting unit 300 or the collecting unit 200 to control the injection position.

The UV irradiating unit 400 is a part of the present invention and is formed in a ring shape and is provided around the nozzle unit 120. The UV irradiating unit 400 irradiates UV light onto the solution sprayed and discharged from the nozzle unit 120. [ As described above, the UV irradiating unit 400 irradiates the polymer solution discharged from the injecting unit 100 with UV to accelerate the curing of the solution. Hereinafter, the specific configuration of the UV irradiating unit 400 will be described in more detail.

FIG. 2 is a cross-sectional view of the nozzle unit and the UV irradiating unit, and FIG. 3 is a detailed view of the UV irradiating unit. 2 and 3, the UV irradiating unit 400 includes a ring frame 410, an optical reflection hole 420, and an optical path forming unit 430.

The ring frame 410 is formed in a ring shape and is provided around the solution discharge hole of the nozzle unit 120. The ring frame 410 may be attached to the nozzle unit 120 in a variety of ways. The ring frame 410 may be welded to the nozzle unit 120, bolted to the nozzle unit 120, Alternatively, the UV irradiation unit 400 may be fixedly coupled to the nozzle unit 120. Alternatively, the UV irradiation unit 400 and the nozzle unit 120 may be assembled and coupled with each other. A structure in which a pedestal is formed around the nozzle unit 120 and the ring frame 410 is placed on the pedestal may be used as a structure for assembling and coupling the UV irradiation unit 400 to the nozzle unit 120 Can be made freely. Of course, this is merely an example, and if the ring frame 410 is stably coupled to the nozzle part 120, the coupling method may be any form.

The light irradiation holes 420 are formed in the shape of a through hole, and a plurality of the light reflection holes 420 are radially arranged along the circumference of the ring frame 410. 3 (A) is a view of the UV irradiating unit 400 viewed from above, and FIG. 3 (B) is a view of the UV irradiating unit 400 as viewed from the bottom. The light irradiation hole 420 is disposed radially below the ring frame 410 along the circumference of the ring frame 410. As shown in FIG. As described above, the plurality of the light irradiation holes 420 are radially arranged to irradiate the solution discharged from the nozzle unit 120 with uniform light irradiation regardless of the direction. .

The optical path forming part 430 is formed of an optical fiber, and one end of the optical path forming part 430 is connected to the light irradiation hole 420 and the other end thereof is connected to a UV light source 450. The light path forming unit 430 forms an optical path such that the UV light emitted from the UV light source 450 is irradiated through the light emitting hole 420. Since the optical path forming portion 430 is formed of an optical fiber, the irradiation direction of the UV light can be easily adjusted as desired regardless of the position of the UV light source 450. [ The other end of the optical path forming part 430 connected to the UV light source 450 is gathered by a separate hole formed in the upper part of the ring frame 410 as shown in FIG. .

2, the UV irradiating unit 400 is formed such that the UV light beams irradiated from the plurality of the light irradiation holes 420 are collected at a point on the central axis of the ring frame 410 , The point is formed to be positioned lower than the discharge position of the nozzle unit 120. By doing so, the solution discharged from the nozzle unit 120 can be efficiently irradiated with UV light to be rapidly cured.

As described above, in the present invention, the UV irradiating unit 400 is formed in a ring shape as a whole, and the light is transmitted through the optical path forming unit 430 made of an optical fiber and irradiated. In this case, the UV irradiating unit 400 of the present invention particularly irradiates the UV light according to the position when the solution discharged from the nozzle unit 120 arrives at the original designed position, so that the UV irradiating unit 400 is rapidly cured at the corresponding position, It is possible to prevent the problem of skewing or skewing more effectively.

In the case of the conventional scaffold manufacturing apparatus, after the polymer solution which had been melted by the high temperature was discharged, heat was naturally released and the solution hardened by the cooling. Since the solution line discharged from the scaffold manufacturing apparatus is very thin, it is possible to produce the cured product by the natural cooling. However, since the solution line is not completely hardened and the solution line of the hot state overlaps There is a risk that a distorted or distorted shape will be created that deviates from the originally intended shape. In order to avoid such a risk, conventionally, the speed of discharging the solution is limited to a certain extent, and as a result, the scaffold production speed is also limited.

However, in the present invention, the curing of the solution can be performed much faster than the curing by natural cooling through UV irradiation. Accordingly, even if the solution discharge rate is further increased, the risk of collapse of the shape is much lower, so that the scaffold production speed can be improved much more than the conventional method.

In addition, in the present invention, since the UV irradiating unit 400 is formed in a small size and attached to the nozzle unit 120, it is greatly advantageous in design, implementation, and operation of an actual scaffold manufacturing apparatus. The advantages of the position and shape of the UV irradiating unit 400 will be described in more detail with reference to FIGS. 2 and 5. FIG.

Generally, in order to irradiate a light beam to a desired position in a light source, a lens for collecting light from the light source, an objective lens for focusing the light to a specific position, a light path for allowing the light from the light source to travel toward the objective lens Such as mirrors, which form the optical system. That is, in the case of such a general optical system, various optical components such as a lens, a mirror, and the like are used to design an optical path, and a space for various optical components required for the optical path is required.

However, since the nozzle unit 120 itself is not a large apparatus in general, there is a limited space for providing the optical unit including the various optical units to the nozzle unit 120. Of course, Also very difficult. In addition, the nozzle unit 120 must continue to move in three dimensions to form a scaffold. When the nozzle unit 120 moves with the optical system attached thereto, the power for moving the nozzle unit 120 And the tendency of dynamic instability due to an increase in weight and momentum becomes more difficult to control such as position and speed. That is, when a UV irradiation unit is constituted as an optical system generally used in the past, design and operation become very difficult.

On the other hand, there were devices which were able to irradiate a separate UV at the processing position in the conventional process (besides the scaffold manufacturing field). Examples of such a configuration are shown in Figs. 5 (A) and (B).

Fig. 5 (A) is to irradiate UV light below the substrate on which the object to be processed is placed. 5 (A), firstly, a waste of resources that will be continuously irradiated with the UV light is applied to the already cured portion, and second, the stage in which the object to be processed is placed is allowed to pass through the UV light There is a limitation that it is required to be made only of a transparent material, and third, there is a problem that the irradiation range is limited because UV light is irradiated from the lower side of the object to be processed. Particularly in the third problem, in particular, the present invention is to produce a three-dimensionally formed scaffold. For example, if a scaffold shape is formed in a stacked form from the bottom, effective curing may be achieved at the bottom layer The higher the probability that UV curing itself can not be achieved due to the UV rays being unable to reach the top of the scaffold (due to the already formed scaffold layer). That is, the method of irradiating the UV light from the lower side of the stage as shown in Fig. 5 (A) has many problems in application to the manufacture of a scaffold.

FIG. 5 (B) shows an external optical system for irradiating the UV light while following the position as the processing position is changed. This method can solve various problems of the method as shown in FIG. 5 (A), but it is very difficult to actually implement and operate it. When the three-dimensional scaffold is manufactured, the nozzle unit 120 does not move in a certain direction, and the movement of the nozzle unit 120 varies depending on the shape to be formed. In order to move the focus position of the light beam in a separate optical system in accordance with the movement of the nozzle unit 120, it is necessary to change the position or angle of various lenses or mirrors constituting the optical system to change the optical path Precise control is required. That is, since the design of the optical system itself becomes extremely complicated and the control thereof becomes very difficult, there are also many problems to realize this.

However, in the present invention, since light is transmitted from the light source through the optical fiber, the complex optical system is not required at all. Therefore, as shown in FIG. 2 and the like, the nozzle unit 120 can be provided as a very small and lightweight apparatus. Since the UV irradiating unit 400 is fixed to the nozzle unit 120 and is moved together with the nozzle unit 120, the UV irradiating unit 400 is moved in the coordinate system with respect to the nozzle unit 120, It is not necessary to change it and it is only necessary to irradiate the light beam with a fixed point. Therefore, there is no need for a complicated control algorithm for changing the irradiation position of the light beam in the UV irradiating unit 400 or a driving device for controlling the UV irradiation unit 400.

As described above, the UV irradiating unit 400 according to the present invention has a simple structure and can easily realize the structure. The UV irradiating unit 400 is formed in a small size and a low weight so that even when the UV irradiating unit 400 moves together with the nozzle unit 120, There is a great merit that it is possible to effectively irradiate the UV light to a desired position without requiring any complicated control design for adjusting the irradiation position of the light with little influence on the control.

4 is a partially enlarged cross-sectional view of the UV irradiating unit, and an additional detailed configuration of the UV irradiating unit 400 of the present invention will be described.

The UV irradiation unit 400 is formed such that the UV rays emitted from the plurality of the light irradiation holes 420 converge at a point on the central axis of the ring frame 410, And is formed to be positioned lower than the discharge position. At this time, when the optical path forming portion 430 is arranged to be directed in the vertical downward direction, for example, a plurality of UV light beams can not converge to one point. That is, it is necessary to provide a configuration that guides the optical path forming portion 430 in a proper direction. Fig. 4 shows embodiments of such a configuration.

4 (A), the optical projection hole 420 is formed so that the end of the optical path forming portion 430 faces the central axis of the ring frame 410, Is formed in a direction inclined at an acute angle with respect to the center axis direction of the ring frame (410). The UV light emitted from the light irradiation hole 420 can be directed toward the central axis of the ring frame 410. [ In addition, by matching the inclined angles of the light reflection holes 420, a plurality of UV light beams can be gathered at one point. In addition, the cross-sectional direction of the light irradiation hole 420 is inclined at an acute angle with respect to the center axis direction of the ring frame 410, so that a point where a plurality of UV rays are gathered can be positioned below the nozzle unit 120.

4 (B), the UV irradiation unit 400 is formed in a pipe shape and is provided inside the ring frame 410, and the optical path forming unit 430 is inserted into the UV irradiation unit 400, And an optical path guide part 440 for guiding the direction of the optical part 430. At this time, the optical path guide part 440 is formed so that the end of the optical path forming part 430 faces the central axis of the ring frame 410, and the extension of the optical path guide part 440 Is formed in a direction inclined at an acute angle with respect to the central axis direction of the ring frame (410). The optical path guide unit 440 allows the UV light to be collected at a point below the nozzle unit 120 on the same principle as in the embodiment of FIG. 4A, Thereby securely securing the directionality of the optical path forming portion 430 and fixing the position and the angle of the optical path forming portion 430 in a stable manner.

The optical path guide unit 440 is hinged to the ring frame 410 so that the angle between the extending direction of the optical path guide unit 440 and the central axis direction of the ring frame 410 can be adjusted . By so doing, it is possible for the user to arbitrarily control how much of the UV light rays are gathered at the lower portion of the nozzle unit 120. FIG.

It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. It goes without saying that various modifications can be made.

1000: Scaffold manufacturing apparatus (of the present invention)
100: injection means 110:
120: nozzle unit 130: heating means
200: collecting unit 300: injection position adjusting unit
310: Fixing bracket 320: Z-axis guide rail
330: X-axis guide rail 340: Y-axis guide rail
400: UV irradiation part 410: ring frame
420: light hole formation 430: light path formation part
440: light path guide 450: UV light source

Claims (9)

A nozzle unit 120 for injecting the solution supplied from the solution storage unit 110, heating means for controlling the temperature of the solution stored in the solution storage unit 110 130), < / RTI > A collecting part 200 formed in a cylindrical shape and provided at a lower part of the injection device 100 to collect a solution injected through the nozzle part 120 of the injection device 100; A jet position adjusting unit 300 for adjusting the jet position by moving the jetting unit 100 or the collecting unit 200 in X, Y, and Z directions; The apparatus for manufacturing a scaffold 1000 according to claim 1,
A ring frame 410 formed in a ring shape, a plurality of optical projecting holes 420 formed on the ring frame 410, a plurality of optical path forming portions 420 formed of optical fibers for guiding light to the optical propagation holes 420, A UV irradiating unit 400 which is provided around the nozzle unit 120 and includes UV irradiation unit 430 for irradiating UV rays onto the solution injected from the nozzle unit 120;
Wherein the UV curing method comprises the steps of:
The apparatus of claim 1, wherein the UV irradiator (400)
The ring frame 410 formed in a ring shape is provided around the solution discharge hole of the nozzle unit 120,
A plurality of the light irradiation holes 420 formed in a through hole shape are radially arranged along the circumference of the ring frame 410,
A plurality of optical path forming portions 430 formed of optical fibers are connected to the light irradiation hole 420 at one end and connected to a UV light source 450 at the other end to couple the UV light emitted from the UV light source 450, Is formed to be irradiated through the light irradiation hole (420).
The apparatus of claim 2, wherein the UV irradiator (400)
The UV rays irradiated from the plurality of light irradiation holes 420 are formed so as to converge at a point on the central axis of the ring frame 410. The point is formed to be positioned below the discharge position of the nozzle unit 120 Wherein the UV curing is performed at a temperature higher than the melting point of the scaffold.
4. The apparatus of claim 3, wherein the light projection hole (420)
The end face of the optical path forming portion 430 faces the central axis of the ring frame 410 so that the cross sectional direction of the optical projecting hole 420 is inclined at an acute angle with respect to the central axis direction of the ring frame 410 Wherein the UV curing is performed using a UV curing method.
The apparatus of claim 3, wherein the UV irradiator (400)
An optical path guide part 440 formed in a pipe shape and provided inside the ring frame 410 and guiding the direction of the optical path forming part 430 by inserting the optical path forming part 430,
The UV curing system according to claim 1,
6. The apparatus according to claim 5, wherein the optical path guide part (440)
The optical path guide part 440 is inclined at an acute angle with respect to the central axis direction of the ring frame 410 so that the end of the optical path forming part 430 faces the central axis of the ring frame 410 And the UV curing is performed in a direction perpendicular to the substrate.
6. The apparatus according to claim 5, wherein the optical path guide part (440)
And is formed to be hinged to the ring frame 410 so as to adjust the angle between the extension direction of the optical path guide part 440 and the central axis direction of the ring frame 410. [ Manufacturing apparatus.
The method of claim 1,
A hydrogel and a UV linker having a property of being cured by UV.
9. The hydrogel according to claim 8,
The present invention relates to a pharmaceutical composition containing at least one selected from the group consisting of PEG (polyethylene glycol), collagen, albumin, amino acid, cellulose, agarose, alginate, heparin, hyaluronic acid ), And chitosan. The apparatus for producing scaffolds according to any one of the preceding claims, wherein the hydrogel is a hydrogel.

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