KR101573595B1

KR101573595B1 - Component Trapping apparatus of silk sericin extract

Info

Publication number: KR101573595B1
Application number: KR1020140013912A
Authority: KR
Inventors: 이시우; 홍영기
Original assignee: 건양대학교산학협력단
Priority date: 2014-02-07
Filing date: 2014-02-07
Publication date: 2015-12-04
Also published as: KR20150093310A

Abstract

The present invention relates to a device for collecting a component of a sericin extract for collecting components contained in the sericin extract for the purpose of evaluating the components of the sericin extract obtained by various methods and comprises a chamber for storing a sample of the sericin extract, A heating water tank for indirectly heating the chamber to extract the components of the sample stored in the chamber; a nitrogen supply tank for supplying the internal nitrogen of the chamber to discharge the components evaporated from the sample; And a collecting tank for collecting the extracted components of the sample contained in nitrogen and discharging nitrogen to the outside, wherein the sample is composed of any one of liquid, solid and powder.

Description

[0001] The present invention relates to a component trapping apparatus for extracting sericin,

The present invention relates to an apparatus for collecting components contained in the sericin extract for the evaluation of constituents of sericin extracts obtained by various methods, and more particularly, to a method for collecting constituents of the sericin extract, which is a polar hydrophilic protein, And extracting and harvesting the sericin extract.

Silk proteins are extracted from silk yarn and consist of two components: fibroin, a crystalline / hydrophobic protein polymer, and sericin, a noncrystalline / hydrophilic protein polymer.

Sericin, which contains more than 30% of serine, a hydrophilic amino acid, is composed of various acidic and basic polar amino acids and contains a large amount of amino acids useful for humans. These properties make sericin have a high moisturizing power, a strong binding force to the skin, a wrinkle preventing function, an antioxidant and tyrosinase activity inhibiting action, and the efficacy as a useful drug such as diabetes has also been reported.

More than 50,000 ton per year of sericin is decomposed in the silk refining process worldwide and discarded as a refining waste solution, and many studies have been actively carried out to recover and commercialize sericin in many countries.

The refining method for removing sericin from silk includes soap refining, soda refining, soap soda refining, and enzyme refining, but this refining makes it difficult to recover sericin by low molecular weight by hydrolysis.

For the purpose of recovering sericin, a calcination method using an acid such as HCl or H ₂ SO ₄ , a high-temperature high-pressure refining method using pure water, an enzyme refining method, and the like are used. Various methods such as a separation membrane for concentration, a catalytic method, and freezing condensation have been utilized.

Despite these various methods, useful sericins such as the natural state can not be recovered because the polar amino acid is denatured by the isoelectric point change in the extraction process and storage state of sericin.

Such destruction of useful components by hydrolysis of sericin has been reported in, for example, Alkali Hydrolysis of Insoluble Sericin, Korean Journal of Sericine Science, Vol. 42 (1), 31-35 (2000)).

Therefore, in order to extract sericin, safely store it, and commercialize it, a technique for easily and accurately measuring the stability of a hydrophilic protein is required. For this purpose, a device capable of capturing components of sericin extract is urgently needed.

SUMMARY OF THE INVENTION It is a first object of the present invention to provide a sericin extract which is obtained by evaporating a component of a sericin extract sample obtained by various methods without chemical change, And a component collecting device.

A second object of the present invention is to indirectly heat serine and threonine, which are chemically unstable in the sericin extract, to evaporate the alcohol component without chemical change and to collect it in a cryogenic condition And to provide an apparatus for collecting components of sericin extract which can accurately evaluate the amount of extracted components and the amino acid denaturation process per hour through GC (Gas Chromatography).

The present invention relates to an apparatus for collecting a component of a sericin extract for collecting components contained in the sericin extract to evaluate the components of the sericin extract obtained by various methods. For this purpose, a sample of the sericin extract A heating water tank for indirectly heating the chamber to evaporate the components of the sample stored in the chamber; a nitrogen supply tank for supplying nitrogen into the chamber to discharge evaporated components from the sample; And a collecting tank for collecting the extracted component of the sample contained in nitrogen and discharging nitrogen to the outside, wherein the sample is composed of any one of liquid, solid and powder.

At this time, the chamber is partially heated and indirectly heated in the heating water tank so as to prevent the amino acid destruction of the sample stored therein, and the heating water tank is further provided with heating means on the outside so as to heat the water stored therein Do.

The collection tank is preferably partially filled in a cooling water tank storing the liquefied nitrogen so that the evaporation component of the sample can be collected in a liquefied state at a cryogenic temperature.

In this case, the collection tank discharges nitrogen and ammonia in a cryogenic atmosphere, and alcohol components in the evaporation component are collected in a liquefied state. The alcohol component is obtained by GC analysis (GC: Gas Chromatography) It is preferable to be configured so as to be able to evaluate.

In addition, it is preferable that the sample is changed to serine, which is a polar amino acid, and glycine, which is nonpolar, from a chemically unstable state of threonine, and the alcohol component is evaporated without chemical change during the process.

According to the composition collecting apparatus of the sericin extract of the present invention, serine and threonine, which are chemically unstable in the sericin extract, are indirectly heated to evaporate the alcohol component without chemical change and collect it at a cryogenic temperature (GC), it is possible to accurately evaluate the amount of the extracted components and the amino acid denaturation process per hour.

In addition, it can be used to evaluate the stability of protein products (pharmaceuticals, supplements, food and beverages, cosmetics, etc.) including aspartic acid and glutamic acid, amino acids having hydroxyl groups such as sericin protein, .

Brief Description of the Drawings Fig. 1 is a configuration diagram of a component collecting apparatus for sericin extract according to the present invention,
FIG. 2 is a graph showing the alcohol component obtained through a component trapping apparatus of the sericin extract according to the present invention by GC (Gas Chromatography). FIG.

Hereinafter, an apparatus for collecting components of sericin extract according to the present invention will be described in detail with reference to the accompanying drawings.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a configuration diagram of an apparatus for collecting components of a sericin extract according to the present invention. FIG.

As shown in FIG. 1, the present invention is a device for capturing the components contained in the sericin extract for the evaluation of the components of the sericin extract obtained by various methods. The sericin extract contains serine, which is chemically unsafe, And indirectly heating threonine to evaporate the alcohol component without chemical change and to collect it at a cryogenic temperature.

The apparatus for collecting components of the sericin extract according to the present invention comprises three parts: a chamber 10, a heating water tank 20, a nitrogen supply tank 30 and a collection tank 40.

The chamber 10 temporarily stores a sericin extract sample obtained by various methods. Here, the sample may be any one of liquid, solid, and powder.

The chamber 10 has a structure in which an inlet pipe 11 for introducing nitrogen and a discharge pipe 12 for discharging evaporation components from the nitrogen and sericin extract are connected.

In addition, the heating water tank 20 indirectly heats the chamber 10 to evaporate the components of the sample stored in the chamber 10.

The heating water tank 20 is further provided with heating means 21 for heating the water stored therein.

The above-described structure changes the polar amino acid serine of the sample and the threonine into glycine, which is non-polar in a chemical unstable state, by indirectly heating the chamber 10 via water, It is a structure in which the alcohol component can be evaporated without being extracted.

In addition, the nitrogen supply tank 30 supplies nitrogen, which is a stabilized gas, to the inside of the chamber 10 through the inlet pipe 11 so that the components evaporated from the sample are discharged together with the nitrogen through the outlet pipe 12 in real time Function.

At this time, the nitrogen supply tank 30 may further include a valve 31 and an air flowmeter 32 so as to block the nitrogen supply or adjust the optimum amount of nitrogen supply.

On the other hand, when the nitrogen and the evaporation component are introduced into the collecting tank 40, the nitrogen and the ammonia in the evaporation component are discharged to the outside, and only the alcohol component is converted into the liquid phase in the cryogenic atmosphere so as to be collected.

To this end, the collecting tank 40 has a structure in which the liquid nitrogen is partially stored in the cooling water tank 50.

Therefore, the collection tank 40 collects only the alcohol component in the evaporated component of the sample in a liquefied state in a cryogenic atmosphere, acquires it, and evaluates the amount of the extracted component and the amino acid denaturation process per hour through GC (Gas Chromatography) .

N 2 (nitrogen) gas at room temperature was purged at 500 cc / min through an airflow meter and supplied for 30 minutes. At this time, water in the heating water tank was tested at a temperature in the range of 40 to 80 占 폚.

time Water temperature in the heating water tank Collection amount (g) 30 minutes 40 ℃ 0.02-1 g 30 minutes 50 ℃ 0.07 to 1.5 g 30 minutes 60 ° C 0.1 to 2 g 30 minutes 70 ℃ 0.5 to 3 g 30 minutes 80 ℃ 0.2 to 2.7 g

As can be seen from Table 1, there was a disadvantage in that the test time was prolonged due to a small amount of capture at 40 ° C, 50 ° C, and 60 ° C for 30 minutes. At 70 ° C and 80 ° C, It was found to be suitable for the subsequent test.

Therefore, the water temperature in the heating water tank of the present invention should be maintained in the range of 70 ° C to 80 ° C.

Extraction method Treated solvent Condition Classification symbol
High-temperature high-pressure method Pure water 120 ° C, 2 hr HTW1 Water + Soap (4g / l) 120 ° C, 2 hr HTW3 NaOH method
Water + 0.05M NaOH (2 g / l) 95 ° C, 2 hr HS1 Water + 0.1 M NaOH (4 g / l) 95 ° C, 2 hr HS2
Na ₂ CO ₃ method
Water + Na ₂ CO ₃ (5 g / l) + soap (4 g / l) 95 ° C, 2 hr CS1 95 ° C, 2 hr CS2
Electrolytic reduction method
Electrolytic reduced water (pH 12.0) 95 ° C, 2 hr ERW1 95 ° C, 2 hr ERW2

As shown in Table 2, the sericin extracts extracted by the high-temperature high-pressure method, the NaOH method, the Na ₂ CO ₃ method, and the electrolytic reduction method were extracted.

After that, a sample of each sericin extract was added to the component collecting apparatus of the sericin extract of the present invention, and only the alcohol component was evaporated.

The amount of alcohol and the amino acid denaturation process were analyzed by GC (Gas Chromatography).

As a result, as shown in Figs. 2 (a) and 2 (b), components such as methanol, isopropyl alcohol, ethanol, and ethylene chloride, and the extraction amount and the amino acid denaturation process per hour were obtained.

By understanding the extraction amount and the denaturation process of these sericin extracts, it can be used for the evaluation of the stability of protein products (medicines, supplements, food and beverages, cosmetics, etc.) including amino acids Serine, Threonine, Aspartic acid and Glutamic acid. In the industry applying the sericin protein extract, the performance evaluation of the inter-process products and the stability of the product can be evaluated.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the invention as defined by the appended claims. It is obvious that you can do it.

10: chamber 11: inlet pipe 12: outlet pipe
20: heating water tank 21: heating means
30: Nitrogen supply tank 31: Valve 32: Air flow meter
40: collection tank
50: Cooling water tank

Claims

In order to evaluate the components of the sericin extract obtained by various methods, there is provided a device for collecting cerasin extracts for collecting components contained in the sericin extract,
A chamber 10 for storing a sample of sericin extract;
A heating water tank (20) indirectly heating the chamber (10) to evaporate the components of the sample stored in the chamber (10);
A nitrogen supply tank 30 for supplying nitrogen into the chamber 10 to discharge components evaporated from the sample; And
And a collecting tank (40) for collecting the evaporation component of the sample contained in nitrogen and discharging nitrogen to the outside,
The sample is composed of any one of liquid, solid and powder,
The chamber 10 is partially heated and indirectly heated in the heating water tank 20 so as to prevent the amino acid breakage of the sample stored therein,
Wherein the heating water tank (20) is further provided with heating means (21) on the outside so as to heat the water stored in the heating water tank (20).

delete

The method according to claim 1,
Wherein the collection tank (40) is partly stored in a cooling water tank (50) in which liquefied nitrogen is stored, so that the evaporation component of the sample can be collected in a liquefied state at a cryogenic temperature.

The method of claim 3,
In the collection tank 40, nitrogen and ammonia are discharged in a cryogenic atmosphere, alcohol components in the evaporation component are collected in a liquefied state, and the amount of the alcohol component is measured by GC (Gas Chromatography) Wherein the composition is capable of evaluating the process.

The method according to claim 1,
Wherein the sample is changed from serine, which is a polar amino acid, and threonine, to chemically unstable and nonpolar glycine, and the alcohol component is evaporated without chemical change in the course of the process. Device.