KR101547587B1 - Producing method for anti-obesity composition using heat-treated ginseng extract and Garcinia Cambogiang and the same composition - Google Patents

Abstract

The present invention relates to a method for preparing an anti-obesity composition using heat-treated ginseng extract and Garcinia cambogia, and an anti-obesity composition prepared by the method, more specifically, a process for extracting ginseng, a process for concentrating ginseng extract under reduced pressure, A process of controlling the pH of ginseng, a process of treating the ginseng with high pH and high pressure and high pressure; A method for preparing an anti-obesity composition using a heat-treated ginseng extract and a method for preparing an anti-obesity composition comprising a step of preparing a concentrated liquid by concentrating ginseng and a step of mixing a ginseng concentrate with a ginseng cambogia, The present invention relates to an anti-obesity composition which increases the content of ginsenoside Rg3 in an anti-obesity composition and provides a simple, practical and advanced production method and minimizes the side effects of Garcinia cambogia.

Description

TECHNICAL FIELD The present invention relates to a method for producing an anti-obesity composition using heat-treated ginseng extract and Garcinia cambogia, and an anti-obesity composition using the same,

The present invention relates to a method for preparing an anti-obesity composition using a heat-treated ginseng extract and a garcinia cambogia, and an anti-obesity composition prepared by the method, more specifically, a process for extracting ginseng, a process for concentrating the extracted ginseng under reduced pressure, A step of adjusting pH of ginseng, a step of treating ginseng with pH adjusted at high temperature and high pressure, A method for preparing an anti-obesity composition using a heat-treated ginseng extract comprising a step of preparing ginseng treated at high temperature and high pressure, a step of concentrating ginseng to prepare a concentrate, a step of mixing Garcinia cambogia with the ginseng concentrate, The present invention relates to an anti-obesity composition that increases the content of ginsenoside Rg3 in an anti-obesity composition produced by the method of the present invention, provides a simple and practical manufacturing method, and minimizes side effects of Garcinia cambogia.

According to the 2009 Statistical Yearbook of the Ministry of Health and Social Affairs, about 50% of Westerners are obese and the social loss due to obesity is estimated to be over $ 1.4 billion. According to the 2005 Statistical Yearbook of the Ministry of Health and Social Affairs, the obesity rate of adults over 20 years old in Korea was 14.8% in 1995 and 31.8% in 2005. In Korea, the westernized dietary habits and the increase of the indoor labor force have caused the obesity population to grow rapidly and become a social problem, and the state is trying to keep the obesity population below 30%.

Obesity is a serious problem because it is transmitted to adult diseases such as hypertension, cardiovascular disease, diabetes, stroke, and dementia. The market for anti - obesity treatment has been attracting attention due to the surge of obesity population and the increase of dieting population, and national policy support for prevention and treatment of obesity and consumer attention. Therefore, many companies are accelerating the development and commercialization of anti-obesity drugs.

Orlistat, sibutramine, and phentermine are examples of commercially available products. Orrust is an inhibitor of fat absorption, sibutramine is an appetite suppressant, pentamin is a psychotropic medicine, and now Orrustat And other products are prohibited from selling due to serious side effects or are in a state requiring attention.

Such anti-obesity agents have been reported to be accompanied by serious side effects such as hypertension, pulmonary artery, and heart disease, thus posing a serious risk to health stability. Orrestat, which is considered to be the safest product at present, is a drug that is concerned about side effects of the gastrointestinal tract such as the kidney or pancreas.

The Korean anti-obesity market is growing at a rapid pace in 2010, reaching W100bn. In 2011 alone, it was reduced to 65 billion, which is not a result of the contraction of the consumption market of anti-obesity drugs, but is a temporary phenomenon caused by the prohibition of sales of sibutramine, which caused serious side effects.

In this situation, many consumers who want a diet have negative emotions about imported anti-obesity drugs, and the demand for anti-obesity substances that are safe and have no side effects is increasing rapidly. For this reason, many researchers at home and abroad are looking for safe antioxidants from natural herbal medicines, and they spend a lot of time and money on them. Among them, studies on green tea extract, Yunnan tea, carotene, various herbs, mulberry leaf, bocca, capsaicin of red pepper, and the like have been conducted, but they are difficult to put into practical use.

Among these substances, Garminia cambogia extract and L-carnitine are examples of substances that have been recognized as significant cost-effective agents developed from natural products. Of these substances, Garcia Cambogia is a substance that the KFDA has certified the functionality of "inhibiting the synthesis of carbohydrates into fat and helping to reduce body fat".

Garcinia cambogia extracts are prescribed at 750-2,800 mg / day, and it is necessary to pay attention to ingestion because there are concerns about side effects such as heart, kidney, poor liver, pregnant women, lactating women and growing children. In addition, Garcinia cambogia extract has not been found satisfactory for weight loss from actual consumers, unlike the results from the developmental stage.

Low-polar ginsenosides of ginseng are known to have the ability to inhibit adipocyte differentiation. In particular, ginsenoside Rg3 is known to exert excellent inhibitory effects on adipocyte differentiation. In order to increase the content of ginsenoside Rg3, there are known methods such as a method using a horseshoe crab, a biotransformation method for treating an enzyme, and the like. This method is complicated and time consuming and has difficulties in practical use. In addition, there is a method in which high-temperature high-pressure is directly applied to roots ginseng (ginseng or red ginseng), but it is inconvenient to undergo a drying process and there is a risk that benzopyrene can be produced in the drying process.

Therefore, there is a need for a simple and practical production method capable of inhibiting the production of benzopyrene while increasing the content of ginsenoside Rg3, and developing an anti-obesity composition which has no adverse effects upon administration and has an excellent ability to inhibit accumulation of body fat.

It is an object of the present invention to provide a simple and practical production method for increasing ginsenoside Rg3 among ginseng components.

It is an object of the present invention to provide a method for producing calorie-reduced ginseng extract by reducing the content of carbohydrates contained in ginseng.

It is also an object of the present invention to provide an anti-obesity composition which is excellent in reducing fertility and minimizing side effects.

The present inventors have far-derived ginseng ginseng research of peculiarities minutes, it was confirmed that the excellent efficacy of removal of intravascular triglycerides are the most problematic of illnesses in animals and clinical trials, efficacy and anti gojihyeol (Tae Myoung Kim et. Al Lab Ae-Jung Kim et al ., Anim. Res.:24: 67-75, 2008; al . Korean J. Food & Nutr. 25: 99-104, 2012). Using these effects, it was estimated that the development of a food for inhibiting the accumulation of body fat, the enhancement of the body fat accumulation inhibiting ability of commercially available anti-obesity products, and the development of adjuvant for reducing side effects.

The extract of Garcinia cambogia is currently dominating the consumer market as an anti-obesity food. This substance has been shown to inhibit the accumulation of fat, has been given by the KFDA fat suppression function. However, Garcinia Cambogia encourages pregnant and lactating women to avoid ingestion, as they can be accompanied by side effects that cause damage to the gastrointestinal tract, including the kidneys and pancreas.

In addition, many consumers who took Garminia cambogia extracts reported that they did not lose weight, and some of them answered that they had some obesity-suppressing effect at the beginning of taking them, but they did not have persistent obesity-suppressing effect when they continued taking them.

Ginseng prepared material confirmed from previous studies of the inventors of the present invention showed excellent efficacy in anti gojihyeol function and blood and reduction of the saturated fatty liver (Tae Myoung Kim et. Al Lab . Anim. Res.:24:67-75, 2008 ;. Ae-Jung Kim et al . Korean J. Food & Nutr. 25: 99-104, 2012), it was confirmed that cancer treatment efficacy is increased and side effects are reduced when used simultaneously as an adjuvant treatment during drug treatment of cancer patients (Goeun Yang et al. Food Sci. Biotechnol. 20: 1649-1653, 2011, Dongsun Park et al Environmental Toxicology & Pharmacology 31: 397-405, 2011).

From these results, it was found that ginseng preparations were mixed with the ginseng preparations in Garcinia cambogia, and as a result, the effect of inhibiting the accumulation of weak body fat in Garcinia cambogia was enhanced, the side effects of cardiovascular diseases such as liver and heart were reduced, Compositions were expected to be possible.

The present invention relates to a method for producing an anti-obesity composition using heat-treated ginseng extract and Garcinia cambogia.

More specifically,

A step of extracting ginseng;

Concentrating the extracted ginseng under reduced pressure;

Adjusting the pH of the concentrated ginseng;

Treating the ginseng with the pH adjusted at high temperature and high pressure;

A step of saccharifying the high-temperature high-pressure treated ginseng;

A step of concentrating the above-prepared ginseng to prepare a concentrated liquid;

The present invention relates to a method for preparing an anti-obesity composition using a heat-treated ginseng extract and a Garcinia cambogia, which comprises mixing the ginseng concentrate with a ginsenoside cambogia.

Further, the step of adjusting the pH is characterized by adjusting the pH to 3.0 to 6.5.

The saccharification step is characterized in that ethanol is mixed and dissolved in the concentrated ginseng solution, and then the saccharide is removed by filtering the solution when a significant amount of saccharide is precipitated.

The ginseng may be at least one selected from the group consisting of white ginseng, red ginseng, white ginseng and red ginseng.

The present invention also provides an anti-obesity composition containing heat-treated ginseng extract and Garcinia cambogia.

The method of preparing an anti-obesity composition using the heat-treated ginseng extract of the present invention and an anti-obesity composition using Garcinia cambogia is simple and convenient in terms of practicality, thereby increasing the content of ginsenoside Rg3, inhibiting the production of benzopyrene, reducing calories Thereby providing a composition having excellent cost-effectiveness.

In addition, the anti-obesity composition of the present invention raises the cost-effectiveness of Garcinia cambogia and has a great effect on the improvement of liver fat, total cholesterol of body fat, and blood ALT, AST, ALP, LDL-C and HDL-C .

In addition, the anti-obesity composition of the present invention has the effect of reducing side effects of existing anti-obesity substances.

Figure 1 shows the dietary composition of diets for obesity (Research Diets).
Fig. 2 shows the change in body weight of the test group (G1, G2, G3, G4, G5) for 6 weeks. Each value was expressed as mean ± standard deviation (N = 10). Significant difference from normal control, ^* P <0.01. Significant difference from obesity control, ^# P <0.05 (Tukey HSD-test).
Figure 3 shows the 6-week weight gain of the test group (G1, G2, G3, G4, G5). Each value was expressed as mean ± standard deviation (N = 10). Significant difference from normal control, ^* P <0.01. Significant difference from obesity control, ^# P <0.01 (Tukey HSD-test).
Figure 4 shows the 6-week dietary efficiency of the test group (G1, G2, G3, G4, G5). Significant difference from normal control, ^* P <0.01. Significant difference from obesity control group, ^# P <0.01. Significant difference from the red hot pepper concentrate test group, ^@ P <0.01 (Tukey HSD-test).
FIG. 5 shows the measurement of adipose tissue volume of the epididymal adipose tissue of the test group (G1, G2, G3, G4, and G5) by H & E staining using an image analyzer. Each value was expressed as mean ± standard deviation (n = 5). Significant difference from normal control ^* P <0.01. Significant difference from obesity control group, ^# P <0.05. Significant difference from obesity control, ^# P <0.01 (Tukey HSD-test).
Fig. 6 shows the epididymal adipose tissue of the test group (G1, G2, G3, G4, G5) observed by H & E staining with an adipocyte size microscope.
FIG. 7 is a microscopic view of the liver taken during the autopsy of the test groups (G1, G2, G3, G4 and G5).

Hereinafter, the configuration of the present invention will be described in detail with reference to specific examples. It is to be understood, however, that the invention is not limited to the disclosed embodiments, but, on the contrary, is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the appended claims.

Selection of raw materials

Ginseng extracts were used in combination with low - cost functional materials with few side effects. As a substance known to help reduce body fat by inhibiting the synthesis of carbohydrates into fat, sodium hydroxy citrate is the main ingredient of Garcinia cambogia extract. The present inventors have selected the powder of Garcinia cambogia extract as a raw material of the present invention.

Next, processed ginseng with excellent inhibition of adipocyte differentiation was selected.

It is an object of the present invention to produce ginseng which, when mixed with Garminia cambogia, a cost effective functional substance to be used in the present invention, increases the anti-obesity effect and reduces side effects. Previous studies have shown that red ginseng, especially ginsenoside Rg3-fortified processed ginseng, such as black ginseng, inhibits adipocyte differentiation. In the following Examples 1 to 3, the processed ginseng, which is most effective for the production of anti-obesity composition, is selected from among white ginseng and red ginseng, which is effective for the control of the anti-obesity composition. And the ginseng raw material to be used in the present invention was selected.

Example  1: white ginseng, red ginseng, White rice ginseng , Hong Mi-sam  Produce

White ginseng production method: Five years old ginseng was purchased from Geumsan market, cleaned with tap water, and dried to produce white ginseng.

Preparation of white ginseng: Only the middle part of white ginseng was cut and used as white ginseng.

Red ginseng preparation: Red ginseng was prepared by ginseng cultivation at 85 ~ 90 ℃ for 8 hours and dried at 50 ℃ for 10 days.

Preparation method of red ginseng: The red ginseng was cut into red ginseng samples.

Example  2: white ginseng concentrate, red ginseng concentrate, White rice germ extract , Red hot pepper concentrate  Produce

1 kg of white ginseng, white ginseng, red ginseng, and red ginseng were weighed and placed in an extraction vessel, and 10 times by weight of 50% ethanol was added and then extracted for 8 hours in a constant temperature water bath at 80 ° C. This extraction procedure was repeated three times, and the supernatant was collected and concentrated with a vacuum concentrator to prepare a concentrate having a solid content of 60%. The samples were used as white ginseng concentrate, red ginseng concentrate, white rice germinated concentrate and red ginseng extract. Each of the concentrates thus prepared was used for measuring the inhibitory effect of adipocyte differentiation It was used as a test substance.

Example  3: in vitro Screening test for inhibitor of adipocyte differentiation

The lipid accumulation inhibitory potency of each concentrate of Example 2 was measured by using 3T3-L1 lipid precursor cells to measure the inhibitory effect of the test material on adipocyte differentiation.

3T3-L1 preadipocytes are cultured in 1% penicillin-streptomycin (. P / S Zenbio Inc. NC USA) , and 10% BCS (bovine calf serum) is added using a DMEM 37 ℃, 5% CO ₂ conditions Lt; / RTI &gt; Cells were washed with phosphate buffered saline (PBS), and then incubated at 37 ° C with 0.25% trypsin-EDTA solution for 5 ~ 6 days at 2-day intervals. 5% CO ₂ After the cells were allowed to stand for 5 minutes under the condition, cells were desorbed and subcultured. To induce differentiation of 3T3-L1 adipose precursor cells, cells were inoculated into each well of a 96-well plate at a density of 0.5x10 &lt; ⁶ &gt; cells / well using DMEM supplemented with 10% fetal bovine serum (FBS) well, and cultured for 4 to 5 days at 2-day intervals until the cells were completely fused with the medium. Fused cells were treated with FBS and MDI solution (0.5 mM 3-isobutyl-1-methyl xanthine, 1 M dexamethasone, 5 g / ml insulin) to induce differentiation. At this time, white ginseng concentrate, white rice germinated concentrate, red ginseng concentrate, and red ginseng concentrate were treated at a concentration of 2.6 μg / l respectively. After 2 days of differentiation, the medium was replaced with DMEM containing 10% FBS, 1% P / S and 5 g / L insulin, and 10% FBS and 1% P / S were included every 3 days from the induction of differentiation Lt; RTI ID = 0.0 &gt; DMEM &lt; / RTI &gt; After differentiation was induced for 7 to 9 days, the fat accumulation rate was investigated by measuring the absorbance by oil red O staining. After completion of induction of differentiation, 10% FBS DMEM was removed, cells were washed twice with PBS, fixed with 10% formalin at 4 ° C for 1 hour, and washed three times with distilled water. The washed cells were stained with oil red O (Sigma Co. St. Louis, Mo., USA) for 1 hour, which specifically reacted with intracellular lipids. After staining, the staining solution was removed, washed three times with distilled water, and then the wells were filled with distilled water and observed under a microscope. The accumulation of triglyceride was determined by removing the distilled water and adding 2 ml of 100% isopropyl alcohol to the completely dry well to elute the oil red O and then measuring the absorbance by using an ELISA reader at 490 nm according to Equation 1, Table 1 shows.

White ginseng concentrate Red ginseng concentrate White rice germ extract Red hot pepper concentrate Fat accumulation rate (%) 88 ± 4 86 ± 5 85 ± 2 82 ± 4

As shown in Table 1, the ability of each concentrate of Example 2 to inhibit adipocyte differentiation was found to be higher in white ginseng concentrate, red ginseng concentrate, white rice germ extract and red ginseng extract than in white ginseng. Therefore, in the present example, red ginseng was selected as a raw material.

Example  4: for inhibition of adipocyte differentiation Extraction and concentration of red ginseng

According to Patent Registration No. 919134, among the ginseng saponins, ginsenosides having a high fat accumulation inhibitory power were low-polar ginsenosides and ginsenosides Rg3. However, the previously known method of increasing ginsenoside Rg3 is complex and time consuming, and there is a risk that benzopyrene is sometimes produced. Therefore, in Example 4, the production process was simple and economical with excellent fat accumulation inhibiting ability.

1 kg of red ginseng was added to an extractor equipped with a reflux condenser, and 10-fold amount of 70% ethanol was added thereto, followed by extraction at 80 ° C for 8 hours. This extraction was repeated twice to collect the supernatant.

After the extraction of 70% ethanol, red hot water was added 10 times of purified water and the extraction was repeated 3 times at 100 ° C for 8 hours to collect the supernatant.

The 70% ethanol extract and the water extract were collected and concentrated under reduced pressure at 50 ~ 60 ° C until the temperature reached 20 ° brix. Ethanol was completely removed in this process.

The concentration of benzopyrene in the concentrate was measured to be 0.08 ppb or less. This amount is less than 0.3 ppb, which is the amount of benzopyran produced in Patent Registration No. 1093899 (the name of the invention: Red Ginseng and Red Ginseng Reduced Method). Therefore, it is a very safe production method in which benzopyrene is hardly produced.

Example  5: pH Test to inhibit adipocyte differentiation of high-temperature and high-pressure treated substances (high-temperature and high-pressure process)

100 g of the 20 [deg.] Brix red hot pepper concentrate was placed in a 500 ml sterilized bottle. Three sample bottles were made. Three kinds of sample bottles adjusted to pH 3.0 to 3.5, 4.0 to 4.5, and 6.0 to 6.5 using citric acid were prepared. The pH adjusted sample bottle was placed in an autoclave and subjected to steam heat treatment at 121 ° C for 3 hours at high temperature and high pressure. The high-pressure high-temperature treated samples were measured for inhibiting adipocyte differentiation by the method of Example 3. The results are shown in Table 2.

pH 3.0 to 3.5 4.0 to 4.5 6.0 to 6.5 Fat accumulation rate (%) 76 ± 5 78 ± 8 80 ± 4

As shown in Table 2, the accumulation rate of adipocyte differentiation was reduced to 80% in 82% (see Table 1) and 76% in 3.0-3.5 pH treatment at high temperature and high pressure treatment. It was found that the treatment of red ginseng adjusted to pH 3 ~ 3.5 at 121 ℃ and high temperature and pressure showed the highest inhibitory effect on adipocyte differentiation.

Example  6: Low calorie for fat accumulation inhibition Red hot pepper concentrate  Manufacturing (sugar production process)

Ginseng contains about 60 to 70% of carbohydrates. This is the source of calories that ginseng has (Korean Ginseng and Tobacco Research Institute, December 1996). The amount of heat of red ginseng 100g was measured to about 330 kcal.

Therefore, it is presumed that the carbohydrate of ginseng is left as it is, and when it is used as a raw material for the depilatory cost, it will not have a great effect on the cost effectiveness.

The mixture was mixed at a ratio of 30 ml of the hot red ginseng extract of Example 5 and 70 ml of 100% ethanol prepared by the high temperature and high pressure treatment, and dissolved well. After the saccharide was precipitated in a considerable amount, the solution was filtered to remove the saccharide.

The solution from which the precipitate was removed was concentrated under reduced pressure at 70 ° C to prepare a concentrated red ginseng extract having a solid content of 60%.

The residual carbohydrate content of the prepared red ginseng extract was measured. The results are shown in Table 3.

High-temperature high-pressure treated red ginseng concentrate Sugar-treated red hot pepper concentrate Carbohydrate content (g / 100g) 45.8 27.6

As shown in Table 3, the carbohydrate contents of the samples before and after the sugar planting process were 45.8% and 27.6%, respectively. As a result of the sugar-processing process, carbohydrates decreased by 18.2%. Calculation of this reduced carbohydrate calorie was found to be reduced by 72.8 kcal per 100 g of concentrate.

The carbohydrate content test method was performed as shown in Equation (2) after measuring the moisture content, ash content, crude fat content (fast Rake extraction drying method) and crude protein content (Kjeldahl nitrogen determination method) of the sample.

It was possible to reduce the heat amount of the red hot pepper concentrated liquid from the minimum 70 kcal to the maximum 110 kcal. This is equivalent to one fifth to one third of the red ginseng heat of 330kcal.

Example  7: Red hot pepper concentrate  Of the product Ginenoside  Pattern change test

Changes in the pattern of ginsenosides were investigated during the high temperature and high pressure treatment and the saccharification process of the ginsenoside of the red ginseng extract.

The ginsenoside analysis was conducted in accordance with the Korean Food and Drug Administration Notification No. 2008-12 (2008, 06, 01) ginsenoside method. 0.5 to 1 g for powder samples and 2.0 to 3.0 g for extract samples were precisely weighed and placed in a 250 ml extraction container. After adding 50 ml of methanol, extraction was carried out in a 50 ° C ultrasonic dispersing bath for 20 minutes. The extract solution was centrifuged at 3000 rpm for 10 minutes, and the supernatant was collected. This procedure was repeated three times. The filtrate was collected, filtered through a membrane filter of 0.4 mu m, and concentrated under reduced pressure at 50 DEG C or lower. The concentrated residue was dissolved in 5 ml of methanol and analyzed for ginsenoside by HPLC / MS. The column conditions were as follows: Xterra MS C18 with an inner diameter of 2.1 mm, a length of 150 mm, a filler size of 3.5 μm, a column temperature of 30 ° C., a flow rate of 0.25 ml / min, and a sample injection volume of 5 μl / The mass detector conditions are shown in Table 5. The results are shown in Table 6.

Red hot pepper concentrate
(mg / g) High-temperature high-pressure treated red ginseng concentrate (mg / g) High-temperature high-pressure treatment,
(mg / g) ginsenoside Rb1 6.810 4.880 6.973 ginsenoside Rb2 2.266 1.238 7.304 ginsenoside Rb3 0.738 0.757 1.070 ginsenoside Rc 3.535 1.893 4.385 ginsenoside Rd 1.057 1.873 3.864 ginsenoside Re 4.951 0.648 3.627 ginsenoside Rf 1.155 2.013 4.918 ginsenoside Rg1 2.824 - 1.183 ginsenoside Rg2 0.619 2.218 5.915 ginsenoside Rg3 0.620 10.348 16.584 ginsenoside Rh1 0.444 2.618 6.642 ginsenoside Rh2 - - - total 25.019 28.486 63.096

As shown in Table 6, the content of ginsenosides Rg2, Rg3 and Rh1, particularly the content of Rg3, increased sharply by adjusting the pH of the red peppermint concentrate to 3 and then treating it at 121 占 폚 under high temperature and high pressure.

From these results, it was confirmed that the polymer ginsenosides (Rb, Rc, Re) and the like were converted into low-grade ginsenosides by desorption and structural conversion due to high heat.

Also, the content of ginsenosides was increased by treating 70% ethanol to remove a portion of the sugar. This is because the total weight is reduced by the amount of saccharified saccharide, and therefore the relative weight of ginsenoside is increased.

In conclusion, the red ginseng concentrate was subjected to high-temperature and high-pressure treatment, and the calories were reduced by one-third, the concentration of ginsenoside was increased by 2.5 times, and the ginsenoside Rg3, which is known to be effective in cost reduction, was increased by about 27 times.

And the red hot ginseng concentrate of this example was similar to the ginsenoside pattern of black ginseng prepared by the cored bean gum or fermentation method. However, the ginsenoside content of the red ginseng concentrate of the present example was characterized by a sharp increase.

From these results, it was judged that the manufacturing process was the most effective method of producing ginsenoside Rg3 enhanced ginseng (black ginseng).

Example  8: Anti-obesity  Composition manufacturing

The results of Examples 1 to 7 were combined to produce an anti-obesity composition.

1 kg of red ginseng was added to an extractor equipped with a reflux condenser, and a 10-fold amount of 70% ethanol was added thereto, followed by extraction at 80 ° C for 8 hours. This extraction was repeated twice to collect the supernatant. After the extraction of 70% ethanol, red hot water was added 10 times of purified water and the extraction was repeated 3 times at 100 ° C for 8 hours to collect the supernatant. The 70% ethanol extract and the water extract were collected and concentrated under reduced pressure at 50 ~ 60 ° C until the temperature reached 20 ° brix.

The red ginseng condensate was adjusted to pH 3.0 with citric acid and subjected to steam heat treatment at 121 ° C and high temperature and high pressure for 3 hours in an autoclave.

The mixture was mixed well at a ratio of 30 ml of the hot water high-pressure treated red hot-water extract solution to 70 ml of 100% ethanol, dissolved well, and then allowed to stand. When a significant amount of saccharide was precipitated, the solution was filtered to remove the saccharide.

The solution from which the precipitate was removed was concentrated under reduced pressure at 70 ° C to prepare a concentrated red ginseng extract having a solid content of 60%.

The red ginseng extract prepared above and Garcinia cambogia were mixed at a ratio of 2 to 3: 1 to prepare an anti-obesity composition.

Experimental Example  : Anti-obesity  Animal testing for the efficacy of the composition

Invention Example 8 In order to confirm the efficacy of the anti-obesity composition, animal tests were conducted.

To investigate the effect of the high fat diet-induced C57BL / 6J rats on body weight control and lipid metabolism improvement of the Example 8 anti-obesity composition, high fat diet was orally administered for 6 weeks and body weight, feed intake and feed efficiency We measured the major organ weight, epididymal fat content, epididymal adipocyte size and blood lipid content after autopsy and analyzed the effect of anti - obesity function comprehensively.

1. Test Method

- Test system

end. Species, strain (supplier): mouse, C57BL / 6N (Koatech, Pyeongtaek, Korea)

I. Number of animals (sex) and weight range at the time of purchase: 60 weeks (male), 15 ± 10% g

All. Number of animals (sex) and body weight at the time of administration: 60 weeks (male), 20 ± 10% g

la. Reason for selection of the test system: In a number of studies, high-fat diet-induced obesity models are known to be suitable models for weight control and obesity studies (Woods et al., 2003), C57BL / 6N mice were selected as the relationship, which is easy and the induction of obesity and hyperlipidemia Ingestion high fat diet accumulate many leading articles and reports related to obesity research compared to other systems (Choi et al ., 2012; Min et al ., 2012).

- Animal laboratory room location and condition

end. Animal Room Number - Area: Chungbuk National University Test and Research Center Conventional area, Room: 218

I. Use cage type and size: PSU, 223 × 267 × 145mm

All. Setting temperature: 20 ± 2 ℃

la. Humidity: 50 ± 20%

hemp. Ventilation system and recovery: 120 times / 5hr

bar. Lighting time and contrast: 12hr / day / night

four. Illumination: 150 ~ 300 / Lux

- Test substances and reagents

end. Obesity feed and test substance

1) The fat 45% kcal composition (D12451, Research Diets) of Research Diets Co., Ltd. of Fig. 1 was used. In the test group, the normal pelleted diet, which is a commercially available feed for rodents, was fed free of obesity induction group and the test group was free of high fat diet.

2) The test substance was used by dissolving the test substance provided by the test applicant in sterilized distilled water.

3) On the day of administration, the preparation was administered on the same day.

- Test group composition

Group ^c) Taking medium Way Diet ^a) term Number of animals G1 NC (normal control group) 10 ml / kg D.W. Oral Normal pellet diet

6 weeks ^d)

10 G2 HF (obesity control) 10 ml / kg High fat diet


10 G3 HFD + B (300)
(Group of red hot pepper concentrate) 300 mg / kg / 10 ml 10 G4 HFD + G (100)
(Garcinina, Cambodia) 100 mg / kg / 10 ml 10 G5 HFD + BG (400)
(Garcinia cambogia + mixture of red hot pepper concentrate) 400 mg / kg / 10 ml 10

^a) Feeds from the G1 group (normal control group) were fed to experimental animal rats (Agribrands Purina Korea Inc. Analysis Service of Central Laboratory, 627 Jangdang-dong, Pyeongtaek, Gyeonggi).

^{b) High-} fat diets (45 kcal% fat) were purchased from commercially available D12451 diet (Research Diets, Inc., New Brunswick, NJ).

^c) HFD + G (100) (obesity control group), HF (obesity control group), HFD + B (300) HFD + BG (400) (administered to obesity control group, 300 mg / kg of red ginseng extract and 100 mg / kg of galsinia extract)

^{d) At the time of} receipt, animals were subjected to a purification process for one week and then separated for 6 weeks.

-Test Methods

end. Quarantine and purification

1) Veterinary quarantine was conducted for general health status of all animals when animals were obtained.

2) One week of purifying period was set up to select healthy animals to be suitable for the test.

I. Group separation

Using an animal that has no abnormality in quarantine, the animals are separated according to the Z-arrangement method based on their body weight. After completion of the group separation, an individual identification card (test name, test group, individual number, sex, Respectively.

All. Method of administration and duration of the test

1) All animals were subjected to a 1-week purifying procedure at the time of arrival, and the animals were divided into two groups with normal pellet diet (G1) and obese diet (G2, G3, G4 and G5) Free ingestion ( ad libitum ).

(G1, G4, and G5) were dissolved in distilled water at a dose of 10 ml / kg, and the test substance was orally administered once a day for 6 weeks, G2) were orally administered with the same amount of DW once a day for 6 weeks.

la. Object identification and breeding management

1) Attach an individual identification card to the breeding box, and use tail marking to identify the individual. The environment of the breeding room is maintained at a constant temperature of 20 ± 2 ^° C, humidity (50 ± 20%) and 12 hours interval (08: 00 ~ 20: 00). In quarantine and purification period, The animals were housed and maintained at a density of 3 animals during the test period.

2) Litter was used for experimental animal soft litter. The breeding box was changed once a week. All equipment and litter used in the room were sterilized by high pressure steam sterilization at 121 ℃ for 20 minutes.

hemp. Weight measurement and measurement of water intake and feed intake

1) To observe the change in body weight during the test period, the test was performed twice a week at the start of the test.

2) after the start of the test to measure the negative, feed intake measured after a certain time once a week (measured weight) after 24 hours was calculated by subtracting the residual amount from the next day geupyeoryang (Lee et al ., 2011).

- Evaluation items

end. Calculation of weight gain and feed efficiency

1) The body weight gain during the total test period was calculated from the initial body weight and the body weight at the end of the test for each test subject, and the average value among the test groups was calculated.

2) The food efficiency ratio (FER) was calculated as the ratio of body weight gain to total feed intake as a percentage (Lee et al. al ., 2011).

FER (food efficiency ratio) = (weight gain during the test period (g / day)) / (food intake during the test period (g / day))

I. Blood biochemical test

1) After the end of the experiment, the blood was fasted for 12 hours. The blood was taken under ether inhalation and the blood collected from each of the abdominal veins was placed in the SST tube and centrifuged at 3000 rpm for 10 minutes at 4 ° C within 2 hours after the blood collection. And then stored at -70 ° C.

2) Serum levels of triglyceride, total cholesterol, low-density lipoprotein cholesterol (HDL) and high-density lipoprotein cholesterol (HDL) were measured and serum biochemical markers such as ALT (alanine transaminase) , Aspartate transaminase (AST), and alkaline phosphatase (ALP) were measured using an automatic hematology biochemical analyzer (HITACHI 7080).

All. Long-term weighing

1) After blood collection, check the internal organs for abnormalities, remove epididymal adipose tissue and liver, wash with physiological saline, remove moisture with filter paper, and measure the absolute weight with electronic scales. The extracted organs calculated the respective relative weights of the fasted body weight before autopsy.

2) Relative organ weight (%) = organ weight (g) / fasting body weight before autopsy (g) × 100 (Han et al ., 2006)

la. Measurement of fat cells around epididymis

1) Epididymal adipose tissue was extracted and fixed in 10% neutral buffered formalin solution. After embedding in paraffin, paraffin block was cut into 4 ㎛ thickness with a microtome. After that, paraffin was removed using xylene, and then hydrophilized with 100%, 95%, 90%, 80%, 70% alcohol and then stained with H & E (hematoxylin and eosin) Were observed with a microscope (Olympus, Japan) (Lee et &lt; RTI ID = 0.0 &gt; al ., 2011.)

2) Measurement of adipocyte size is well known as an effective method to demonstrate anti-obesity efficacy (Park et al al ., 2005), six adipocyte sizes per sample were determined by light microscopy in an image analysis system (IPKR-1003, Saramsoft Co., Ltd., Korea) to calculate the average lip size of each group (Yun et al ., 2007, Seo et al., 2009).

hemp. Histopathologic examination of liver and lipid deposition

Histopathologic findings of hepatocytes obtained at the time of autopsy are examined by H & E staining for 5 randomly selected test groups. The H & E staining procedure followed the liposuction method described above.

four. Statistical analysis

All experimental results are expressed as mean ± standard deviation, and Levene's test is used to compare variance homogeneity for all data. ANOVA (one-way analysis of variance) Dunnett's t-test was performed to determine the test group with significant differences from the control group.

2. Test Results and Considerations

1) General symptoms and weight changes

(1) General symptoms: No symptoms other than weight gain were observed during the study.

(2) Change in body weight: as shown in Fig.

As shown in FIG. 2, the body weight of the experimental animals was measured for 6 weeks, and it was confirmed that obesity was induced in the obesity control group HF compared with the NC group ( p <0.01).

The HF group showed a significant increase in mean body weight from the 7th day to 41 days after the end of the experiment ( p <0.01).

On the other hand, the mean body weight of BG (400) group was 21.23 ± 0.34g at the 3rd day after administration, and 22.58 ± 0.33g of HF group showed significant weight gain inhibition compared with that of HF group. ( p < 0.01).

The mean daily body weight of the B (300) and G (100) groups was 22.69 ± 0.40g and 22.62 ± 0.30g, respectively, which was significantly lower than the 24.63 ± 0.46g of HF group. Body weight loss was significantly maintained by day ( p <0.01).

BG (400) and BG (400) groups showed a significant inhibition of body weight gain compared to the HF group in the test group in the B (300) and G (100) It was confirmed that the expression of the weight-lowering effect progressed more rapidly when the inventive composition was administered.

2) Weight gain

We compared the body weight gain of the test group (G1, G2, G3, G4, G5) for 6 weeks. The results are shown in FIG.

Compared with the NC group and the HF group, the HF group showed significant weight gain ( p <0.01).

Compared with the HF group and the test substance administration group, the body weight gain rate was significantly lower in the test substance-administered group throughout the test ( p <0.01).

In the test substance group, the body weight gain rate tended to decrease in the order of B (400), G (100) and B (300) in the order of mixture group of Garcinia cambogia and red ginseng extract It looked.

Body weight of 39.63%, 51.34% and 55.37% in B (300), G (100) and BG (400) groups were decreased when the body weight of the HF group and the test substance administration group were compared at the 42nd day of the test.

The three test substances used in this study were found to have anti-obesity effects by comparison with HF. The intensity of their anti-obesity effect was found to be a mixed substance group (BG (400)) of Gardiania cambogia and red hot pepper concentrate, Garcinia cambogia (G (100)) and red ginseng concentrate (B (300)).

From the above results, it can be concluded that the mixed composition of the final product of the present invention, Garnia cambogia and the red ginseng extract, shows a weight loss higher than that of the case of using the extract of Garcinia cambogia alone, so that the concentration of the red ginseng extract increases the complementary efficacy .

3) Average weekly dietary intake

The average weekly dietary intake of the test group (G1, G2, G3, G4, G5) for 6 weeks was compared. The results are shown in Table 8.

^** P <0.01 compared with normal diet group

^## P <0.01 compared with high fat diet group

Compared with the NC group, there was a significant decrease in mean feed intake in both groups ( p <0.01). There was no significant difference between HF group and HF group in all test groups except for feed intake at 2 and 6 weeks in group B (300). The NC group showed higher intake than the HF group consuming the high fat diet because the high fat diet was relatively high in calories.

4) Dietary efficiency

The dietary efficiency of the test group (G1, G2, G3, G4, G5) was compared for 6 weeks. The result is shown in Fig.

Dietary efficiency (FER) is the daily weight gain divided by the daily dietary intake. Low dietary efficiency is an indicator of lower body weight gain compared to high feed intake.

The calculated dietary efficiency was significantly higher in the HF, B (300), G (100) and BG (400) groups than the NC group ( p <0.01).

( P <0.01) in the test group compared to the HF group, which is an inducer of obesity.

The HF group showed the highest efficiency in the NC group (0.025 ± 0.002), the HF group (0.101 ± 0.005), the B group (0.03 ± 0.005), the B group (0.04 ± 0.005) As a result of the increased dietary efficiency of 75.2% compared to the army, the obesity feed was judged to be the cause of weight gain.

Feed efficiency of the HF group compared to anti-obesity material group is B (300) the group is 36.6%, G (100) the group is 51.5%, BG (400) group showed a decrease results in 56.4% (p <0.01).

These results confirmed that all three anti - obesity substances have the ability to inhibit weight gain. Especially, the highest inhibition rate of weight gain was observed in the mixed composition treatment group of Garcinia cambogia and red ginseng extract. Therefore, it was confirmed that the red ginseng concentrate contributes synergistically to the increase of weight gain suppression of Garcinia cambogia.

5) Relative organ weight measurement

After 6 weeks, the relative organ weights of the test groups (G1, G2, G3, G4, and G5) were measured and compared. The results are shown in Table 9.

^* P <0.05 compared with normal diet group

^** P <0.01 compared with normal diet group

^# P <0.05 compared with high fat diet group

^## P <0.01 compared with high fat diet group

Fat accumulation around the epididymis: Many studies have reported that dietary composition, especially fat intake, has a greater effect on body fat accumulation than energy intake (Miller et al ., 1990). In the present study, the relative weight (%) of epididymal fat around the body weight was 5.55 ± 0.61% in the HF group and 270.0% in the NC group, compared with 1.50 ± 0.99% in the NC group and 4.09 ± 0.91% 3.33 ± 1.27% in the G (100) group and 3.19 ± 0.75% in the BG (400) group were decreased by 35.7%, 66.7% and 74.0%, respectively, when compared with the relative weights of the epididymal fat around the HF group. All groups (HF, B (300), G (100), and BG (400)) consumed high fat diets were significantly higher than the NC group ( p <0.01) (Group B (300), group G (100), group BG 400) were significantly lower than those of HF group ( p <0.01). Since the accumulation of visceral fat is known to be an important factor causing various metabolic diseases, the test compound, especially the mixture of the red ginseng extract and the garnia cambogia, has an excellent inhibitory effect on epididymal fat accumulation and broadly inhibits visceral fat accumulation and effectively prevents the metabolic disorder caused by the high fat diet .

Relative Weight of Liver: The reason that the relative weight of liver increases is the result of accumulation of cholesterol and fat in the liver. The relative weight of the HF group versus the NC group is also high in this study. This is consistent with the results of the high fat dietary allowance. The relative weight of BG (400) group, G (100) group and B (300) group was lower in the test substance administration group when HF group and test substance administration group were compared. From these results, all three kinds of anti-obesity substances used in this study were able to inhibit liver fat accumulation. In particular, a composition of Garcinia cambogia and red hot pepper concentrate showed the most effective inhibitory effect on liver fat accumulation.

Relative weight of the kidneys: The relative weight of the kidneys was lowered by the high fat diet, which was thought to be due to the absence of height increase compared to the increase in body weight. There was no significant difference in kidney weight among the test materials, but the relative height of the kidneys was higher than that of the HF group as a whole.

Relative weight of spleen: The relative weight of the spleen was not significant between the NC group and the HF group, and between the HF group and the test substance administration group.

In conclusion, it was proved indirectly by the test substance treatment that the excellent effect of lowering the relative weight of epididymal fat, relative weight of liver and relative weight of kidney, And it was confirmed that the cost-effectiveness of the developed material is excellent.

6) Blood biochemical exponential test

The contents of ALP, AST, ALT and lipid profiles (HDL, LDL, TC, TG) in plasma of each test group (G1, G2, G3, G4 and G5) were measured.

^* P <0.05 compared with normal diet group

^** P <0.01 compared with normal diet group

^# P <0.05 compared with high fat diet group

^## P <0.01 compared with high fat diet group

^@ P < 0.05 Compared with test group of red ginseng extract

^@@ P <0.01 Compared with test group of red hot pepper concentrate

AST and ALT activities in serum are related to hepatic enzymes. When hepatocellular toxicity is caused by high fat diet, high cholesterol diet, alcohol, etc., hepatic cell damage occurs and hepatic cells are destroyed and the release of these enzymes into blood is enhanced. (Plaa and Charbonneau, 1994), and even in the presence of acute renal failure, hyperlipidemia and pulmonary infarction, elevated levels of ALT, AST and ALP levels in the serum result in elevated levels of bile acid excretion in the liver, It is known that the serum cholesterol content is increased (Kim, 1990).

ALP activity: HF group compared with NC group showed a significant increase of activity (42.3%) ( p <0.01). In the test group, activities of B (300), G (100) and BG (400) were 262.89 ± 34.67 IU / l, 254.91 ± 16.72 IU / l and 240.34 ± 33.14 IU / , 19.0, and 23.6%, respectively ( p <0.01).

AST and ALT activity: The AST and ALT activities of the test substance group were similar to those of the HF group, but the significance was only observed in the BG (400) group (AST: p <0.01, ALT: p < 0.05).

Total cholesterol (T-CHO) content: Total cholesterol (T-CHO) was the highest in the HF group compared with the NC group (93.52 ± 3.87 mg / dL, 156.48 ± 8.41 mg / dL, 67.3% , And high cholesterol diet ( p <0.01). In the comparison with the HF group, it was 138.12 ± 16.96 mg / dl, 133.71 ± 17.29 mg / dl, and 120.32 ± 13.05 mg / dl in the group B (300), G (100) ( P <0.001), but the BG (400) group showed a significant decrease compared to the B (300) group, indicating that the highest T-CHO ( P <0.05), respectively.

TG content: The TG content was 43.56 ± 4.45mg / dl in the HF group (105.9%) compared with 21.16 ± 2.82mg / dl in the NC group (p <0.01). In comparison with HF group, B (300), G (100) and BG (400) groups were 30.09 ± 4.16mg / dl, 28.73 ± 4.31mg / dl and 25.31 ± 3.61mg / , And 41.9%, respectively (P <0.01). These results confirm that the test substance effectively inhibits lipid accumulation with the same tendency as in T-CHO. BG (400), G (100) and B (300) showed the highest inhibitory effect of TG on BG (400).

LDL-C content: Total cholesterol in the same pattern as the result of increased 179.8% to 7.05 ± 0.48mg / dl in comparison HF group and 2.52 ± 1.57mg / dl in the NC group was confirmed by the highly significant (p <0.01) . In the HF group, B (300), G (100), and BG (400) groups were 5.78 ± 1.04 mg / dl, 5.04 ± 1.29 mg / dl and 4.21 ± 1.13 mg / , And 40.3% ( p <0.01), respectively. The BG (400) group showed a significant decrease compared to the B group (300) ( P <0.01), respectively.

HDL-C content was significantly lower in HDL-C group than in NC group (73.68 ± 5.40mg / dl, 51.43 ± 4.09mg / dl in HF group) ( p <0.01). Dl, 63.61 ± 4.02 mg / dl and 70.71 ± 10.62 mg / dl in the B (300), G (100) and BG (400) , And 37.5%, respectively ( p <0.01).

Serum Lipid Levels (HDL-C, LDL-C, T-CHO, TG) were analyzed by BG (400) (300), respectively. These results were similar to those of hepatic enzyme activity index. Among them, BG (400) group showed the most improved value compared to HF group among all test groups. From the above biochemical markers, three kinds of test materials used in this study showed improvement of blood biochemical index, among which BG (400) group showed the best value. It is possible to improve the biochemical indicators of various blood by mixing the red ginseng extract with the mixture of the red ginseng extract rather than using Garcinia cambogia alone as an anti-obesity material, and it can reduce the risk of obesity-induced cardiovascular diseases Respectively.

7) Epididymal adipose tissue size and histological examination

The fat cell sizes of the test groups (G1, G2, G3, G4 and G5) were measured, and the results are shown in FIG. 5, FIG. 6 and Table 11.

Each value is expressed as mean ± standard deviation (n = 5).

^** P <0.01 compared with normal diet group

^# P <0.05 compared with high fat diet group

^## P <0.01 compared with high fat diet group

Long-term high-fat intake is known to induce adipocyte proliferation (DeFronzo 2004), and measurement of adipocyte size is known to be an effective method to demonstrate anti-obesity efficacy (Park et al al ., 2005).

The epididymal adipose tissue belonging to white adipose tissue was observed under a microscope through H & E staining and the results of measurement of adipocyte size using an image analyzer were shown in FIGS. 5, 6 and 4.

The adipocyte size of the HF group consuming the high fat diet increased significantly ( p <0.01) compared to that of the NC group consuming the normal diet, (100) group and BG (400) group) (Figs. 5 and 6).

The relative fat cell size of the epididymal adipose tissue of each group was compared to 1.0 in the HF group and 0.09 in the NC group. It was confirmed that the accumulation of epididymal adipose tissue was most abundant in the HF group . In contrast, BG (400), BG (400), and BG (400) groups were significantly decreased by 0.68, 0.63, and 0.56 times, respectively. The most effective inhibition of adipocyte proliferation was observed ( p <0.01, Table 4).

8) Histopathological evaluation

Each test group (G1, G2, G3, G4, G5) was autopsied and the liver tissue was evaluated. The results are shown in FIG.

Histopathologic findings of the liver during autopsy were more common in the HF group than in the NC group. This is caused by the infiltration of lipids in hepatocytes, which is frequently observed in fatty liver of obese patients. These results are in agreement with the increase in the relative weight of HF.

Lipid accumulation was observed in the experimental group (group B (300), group G (100), group BG (400)). Although some observations were made, no significant fear was observed in the HF group.

In the BG (400) group, lipid infiltration was hardly observed and it was confirmed that fatty liver caused by obesity was remarkably alleviated.

From the above test results, the relative increase / decrease rate (%) of various measurement results of the test substance group relative to the obesity control group (HF) is summarized in Table 12.

B (300) G (100) BG (400) weight -39.6 -51.3 -55.4 Dietary efficiency -36.6 -51.5 -56.4 Fat around epididymis -35.7 -66.7 -74.0 ALP -16.4 -19.0 -23.6 AST -15.5 -15.8 -24.2 ALT -5.9 -7.0 -13.3 HDL-C +12.8 +23.7 +37.5 LDL-C -18.0 -28.5 -40.3 T-CHO -11.0 -14.6 -23.1 TG -30.9 -34.0 -41.9

3) Conclusion

In conclusion, obesity induction group, HF group, was proved to induce obesity by accumulation of fat in body by long-term intake of excess energy included in fat diet as compared with NC group, which is a general diet group.

BG (300), G (100), and BG (400) groups were divided into three groups according to body weight, weight gain, daily feed intake and dietary efficiency, blood biochemical data, relative organ weight, And color were improved in comparison with HF group, which is an inducer of obesity.

In both groups, significant reductions in body weight gain and decreased dietary efficiency over a 6 - week period were demonstrated, demonstrating the ability to inhibit weight gain. Blood biochemical tests showed improved serum lipid levels (HDL, LDL, T-CHO, TG) and improved liver function (ALP, AST, ALT) due to long-term high fat intake compared to HF .

The test substance was found to alleviate liver fatty degeneration accompanied by a decrease in the average size of epididymal adipocytes in epididymal adipose tissue size and histologic evaluation results.

Overall, the efficacy of the test was confirmed in all test substances, and the improvement effect was excellent in the fatty liver, the fat cell size around the epididymis, and the biochemical index of the blood.

The degree of improvement was higher in BG (400) group, G (100) group and B (300) group.

Especially, when mixed with red ginseng concentrate and Garcinia cambogia, it was more effective than Garcinia cambogia or red ginseng extract alone, and liver fat level, epididymal fat accumulation, blood T-CHO, ALP <AST. ALT, LDL-C levels, and HDL-C levels.

Therefore, the BG (400) developed in this study proved to have an efficacy exceeding the potency efficacy of Garcinia cambogia.

In addition, calorie reduction and Ginsenoside Rg3 enriched red ginseng concentrate are considered to be excellent substances for reducing side effects by improving cardiovascular health indicators such as liver and blood, and improving the cost-effectiveness of Garcinia cambogia used as a main material of the present development material. .

Manufacturing example

end. After purchasing the raw materials carefully selected, they are cleaned.

I. Bokbunja extract - Purified water equivalent to 50 times of the raw material is added, and then extracted at 90 to 95 ° C for 48 hours.

Licorice extract - Purified water equivalent to 100 times of the raw material is added, and then extracted at 90 to 95 ° C for 48 hours.

All. Red hot pepper concentrate

      1) Hongmisam manufacturing process: Only the middle and lower fruits of ginseng were collected, washed thoroughly with tap water, boiled for 4-8 hours at 85 ~ 90 ℃, dried for 2 ~ 3 days in 30 ℃ ~ 35 hot air dryer, Dry in sunlight (less than 0.2 ppb in the benzopyrene test).

      2) Extraction step of red ginseng: 50% ethanol of red ginseng 10 times is added and extracted three times for 8 hours at 50 ~ 70 ℃. After that, 10 times more purified water is added and extracted three times at 90 to 95 ° C. Six batches of extracts are collected and concentrated under reduced pressure at 50 ° C to give a 20 ° brix solution. At this time, ethanol should be completely removed.

      3) Preparation of red ginseng extract with ginsenoside Rg3 amplification: Adjust the extract to pH 3 ~ 3.5 with citric acid, then place in 121 ℃ autoclave and boil for 2-3 hours.

      4) Preparation stage of red peppermint concentrate with Karori reduction: 50 ml and 100 ml of ethanol were added to the red pepper extract at 121 ° C. After homogenizing the red pepper concentrate, it was matured for 2 ~ 3 hours and centrifuged or filtered To remove the precipitate. The precipitated solution is collected and concentrated under reduced pressure at about 50 ° C to give a solid content of 60 to 65%, thereby preparing a red hot ginseng concentrate having enhanced calorie-reduced ginsenoside Rg3.

la. The raw materials are put into a stirring tank at a mixing ratio shown in Table 13, and then stirred sufficiently.

Raw material name ratio(%) Bokbunja extract (solids content over 2%) 47.80 Licorice extract (solids content 1% or more) 47.80 Red hot pepper concentrate (solid content more than 60%) 2.96 Garcinia Cambogia 1.30 Citric acid 0.14 Sum 100

hemp. Sterilize at 87 캜 for 1 hour or more.

bar. After the filter is treated in a stirring tank, it is filled.

four. Pack the finished product into a polyethylene film pouch with 70 ml.

Ah. Test the packaged product according to the standards and specifications, and confirm the suitability before shipment.

Claims (6)

A step of extracting ginseng;
Concentrating the extracted ginseng;
Adjusting the pH of the concentrated ginseng extract to pH 3.0 to 6.5;
Subjecting the pH-adjusted ginseng concentrate to high-temperature high-pressure treatment;
A saccharification step of removing the saccharide of the ginseng concentrate treated at high temperature and high pressure;
A step of concentrating the above-prepared ginseng to prepare a concentrated liquid;
And mixing the ginseng concentrate with a ginsenoside cambogia; and a method for producing an anti-obesity composition using the ginseng cambogia.
delete The method according to claim 1,
The method for producing an anti-obesity composition using the heat-treated ginseng extract and the Garcinia cambogia, wherein the saccharification process comprises mixing and dissolving ethanol in a ginseng concentrate, and then allowing the saccharide to settle and then filtering out the saccharide.
The method according to claim 1,
Wherein the ginseng is at least one selected from the group consisting of white ginseng, red ginseng, white ginseng, and red ginseng, and a method for preparing an anti-obesity composition using Garcinia cambogia.
A food composition for improving obesity comprising a heat-treated ginseng extract and a Garcinia cambogia prepared by the method of claim 1, claim 3 and claim 4, wherein the saccharide is reduced. delete

Images