KR101543210B1 - Methylobacterium aquaticum for Odorless Bioflim-Coated Evaporator Core and uses thereof - Google Patents

Abstract

The present invention relates to a microbial methyl bromide microorganism used for coating with an odorless biofilm which does not cause odor in an air conditioning apparatus Aqua tikum (Methylobacterium The present invention relates to an odorless EVA core which does not cause odor in an air conditioner coated with a biofilm using the same.
The odorless microorganisms and the odorless eva cores of the present invention can inhibit the growth of microorganisms causing odors in the air conditioner, thereby providing a pleasant indoor environment.

Description

Methylobacterium aquaticum for Odorless Bioflim-Coated Evaporator Core and uses thereof

The present invention relates to an odorless evacore coated with a biofilm with odorless microorganisms in order to inhibit the proliferation of odor-causing microorganisms in an air conditioner, and a method for producing the same, and a microorganism methylobacterium that does not cause odor to evacore. It is about Aquaticum.

Clean air is recognized as the basis for human health and well-being. For example, there are two important factors that can lead to unsatisfactory indoor air quality in enclosed buildings. One is the building itself, which causes large amounts of indoor air pollutants to be removed or diluted, and the other is the generation of odors caused by human activities.

The air conditioning system is a system that lowers the indoor temperature and optimizes the indoor environment by aiming at air conditioning that harmonizes the temperature, humidity, airflow and cleanliness of the air. The penetration rate of these air conditioning systems is increasing gradually due to the improvement of living standards. With the increase in the penetration rate of air conditioning systems, there have been many developments in basic functions, but there are still many problems to be solved in terms of the environment for indoor air quality.

Among the air conditioning systems, particularly, the cause of air conditioner odor is known to be air conditioner odor as metabolites of mold and bacteria, but specific data on what metabolites and how much the relevant mold and bacteria secrete specifically are not yet known. .

Due to the structure of the air conditioner, all the air that has passed through the blower passes through the Evacore.When the cold refrigerant and air are exchanged, condensate condensation occurs on the surface of Evacore due to the temperature difference, and if this condensate condensation continues, the Evacore surface It provides a good environment for fungi and bacteria to inhabit and propagate. In a state in which mold and bacteria proliferate in Evacore exposed to the outside air, volatile organic compounds (mVOCs) of microorganisms are generated as metabolites of bacteria that proliferate on the surface of Evacore, and the air passing through Evacore is blown into the room. Then, at this time, due to volatile organic compounds generated by microorganisms, indoors may be exposed to odors caused by molds and bacteria during long-term use.

The surface of Evacore, where odor occurs, is covered with a biofilm according to long-term use, and these are composed of bacteria, cell clusters, and EPS, and EPS is protein, polysaccharide, polyuronic acid, and nucleic acid. It contains various components such as (Nucletic) and lipid. On the surface of Evacore, various bacteria and fungi proliferate using the biofilm as a nutrient, and as a metabolite, organic substances (mVOCs) by microorganisms are released. Among the many factors that cause air conditioner odor, it is known as odor.

Accordingly, the present inventors disclosed a biofilm formed of odorless or fragrant specific microorganisms on the surface of Evacore to prevent the deposition and propagation of bacteria and molds that cause odor on the surface of Evacore in Korean Patent Application Publication No. 10-2012-0020309. Invented EVA core coated with a biofilm layer to be coated.

However, in the above invention, the identification of which bacteria is an odorless microorganism was insufficient.

Matters described as the above-described background art are only for enhancing an understanding of the background of the present invention, and should not be taken as acknowledging that they correspond to the prior art already known to those of ordinary skill in the art.

Republic of Korea Patent Publication No. 10-2012-0020309

Therefore, the present inventors studied which microorganisms can be used as odorless Evacore when coated with a biofilm as odorless microorganisms in order to suppress the proliferation of odor-causing microorganisms in the air conditioner. Microorganism methylobacterium Aquaticum was confirmed.

The present invention is a microorganism methylobacterium used for coating with an odorless biofilm that does not cause odor in an air conditioner. To provide an aqua tikum HKMC-1 (Methylobacterium aquaticum HKMC- 1) (KCCM11325P) and for that purpose.

The present invention is methylobacterium Aquaticum HKMC-1 ( Methylobacterium to provide a aquaticum HKMC-1) (KCCM11325P) which does not cause the smell of the air conditioner in the biofilm coating the core odorless EVA for that purpose.

The present invention

(i) Methylobacterium on the surface of Evacore Aquaticum HKMC-1 ( Methylobacterium aquaticum HKMC-1) (KCCM11325P) applying a favorable medium for propagation;

(ii) a methyl on the surface of the medium is applied tumefaciens Affixing the aqua tikum HKMC-1 (Methylobacterium aquaticum HKMC- 1) (KCCM11325P); And

(iii) the methylobacterium Aquaticum HKMC-1 ( Methylobacterium a cultivation step of breeding aquaticum HKMC-1) (KCCM11325P) to form a biofilm;

It is an object of the present invention to provide an odorless EVA core manufacturing method that does not cause odor in an air conditioner comprising a.

Other objects and advantages of the present invention will become more apparent by the following detailed description, claims and drawings.

According to one aspect of the present invention, the present invention is a microorganism methylobacterium used for coating with an odorless biofilm that does not cause odor in an air conditioner. Aquaticum HKMC-1 ( Methylobacterium aquaticum HKMC-1) (KCCM11325P) is provided.

According to another aspect of the present invention, the present invention is methylobacterium Aquaticum HKMC-1 ( Methylobacterium aquaticum HKMC-1) (KCCM11325P) This provides an air conditioner that does not cause an odor in odorless EVA core coated with a biofilm.

According to another aspect of the present invention, the present invention

(i) Methylobacterium on the surface of Evacore Aquaticum HKMC-1 ( Methylobacterium aquaticum HKMC-1) (KCCM11325P) applying a favorable medium for propagation;

(ii) a methyl on the surface of the medium is applied tumefaciens Affixing the aqua tikum HKMC-1 (Methylobacterium aquaticum HKMC- 1) (KCCM11325P); and

(iii) the methylobacterium Aquaticum HKMC-1 ( Methylobacterium a cultivation step of breeding aquaticum HKMC-1) (KCCM11325P) to form a biofilm;

It provides an odorless EVA core manufacturing method that does not cause odor in an air conditioner comprising a.

Features and advantages of the present invention are as follows.

(i) The present invention is a microbial methylobacterium used for coating with an odorless biofilm that does not cause odor in an air conditioner. Aqua tikum HKMC-1 (Methylobacterium aquaticum HKMC- 1) (KCCM11325P) and that do not use them to induce within the odor was coated with a biofilm air conditioner relates to an odorless EVA core.

(ii) The odorless microorganisms and the odorless Evacore of the present invention can suppress the propagation of odorous microorganisms in the air conditioner to remove odors indoors.

1 is a sterilization of aluminum pins and dipping in a nutrient medium containing dust, and then methylobacterium Aquaticum HKMC-1 ( Methylobacterium This is a photograph of a petri dish for inoculating aquaticum HKMC-1)(KCCM11325P).

According to one aspect of the present invention, the present invention is a microorganism methylobacterium used for coating with an odorless biofilm that does not cause odor in an air conditioner. Aquaticum ( Methylobacterium aquaticum ) is provided. More preferably, the microorganism used for coating as an odorless biofilm is methyllobacterium The aqua tikum HKMC-1 (Methylobacterium aquaticum HKMC- 1) (KCCM11325P). Bonn methylobacterium Aquaticum was deposited with the Korea Microbial Conservation Center on November 14, 2012. The methylobacterium Aquaticum HKMC-1 ( Methylobacterium aquaticum HKMC-1) (KCCM11325P) in addition to the bacterium methyl Solarium beuraki Atum HKMC-2 (Methylobacterium brachiatum HKMC-2) (KCCM11326P) , a bacterium methyl Solarium Platani HKMC-3 ( Methylobacterium platani HKMC-3) (KCCM11327P) , Acinetobacter Jones you HKMC-4 (Acinetobacter johnsonii HKMC-4)(KCCM11328P), Bacillus Betnamensis HKMC-5 ( Bacillus vietnamensis HKMC-5)(KCCM11329P), Brevibacillus Invoke Car Bluetooth HKMC-6 (Brevibacillus invocatus HKMC-6) (KCCM11330P) , Day Noko kusu blood kusu HKMC-7 (Deinococcus ficus HKMC-7) (KCCM11331P), grapefruits Sonia Soli HKMC-8 ( Leifsonia soli HKMC-8) (KCCM11332P), Pseudomonas nitro Lowry dew sense HKMC-9 (Pseudomonas nitroreducens HKMC-9) (KCCM11333P) and Sphingomonas Aqua Antilles HKMC-10 (sphingomonas aquatilis HKMC-10) (KCCM11334P) and methyl tumefaciens Coma has been deposited with the state HKMC-11 (Methylobacterium komagatae HKMC- 11) (KCCM11335P) November 14, 2012 in South Korea Culture Center of Microorganisms.

An object to which the biofilm of the present invention can be coated is a cooling device, and the cooling device includes a compressor, a blower, an evacore, and the like, and in particular, an object capable of coating the biofilm of the present invention is an evacore. The air conditioner can be applied to all of the Evacore of any air conditioner, such as a home air conditioner or an automobile air conditioner. More preferably, it can be used in Evacore of a cooling device for automobiles.

The temperature of the indoor air is lowered due to the cooling action of Evacore, and moisture in the air is condensed to generate moisture, and the moisture generated at this time, that is, the condensed water, is discharged to the outside of the vehicle through the exhaust structure inside the Evacore. , It is possible to control the removal of odor when air is introduced into the vehicle through the control of the odor microorganisms of Evacore.

That is, odorless microorganisms are propagated in advance so as to suppress the reproduction of odorous microorganisms.

According to another aspect of the present invention, the present invention is methylobacterium Aquaticum HKMC-1 ( Methylobacterium aquaticum HKMC-1) (KCCM11325P) This provides an air conditioner that does not cause an odor in odorless EVA core coated with a biofilm.

In addition, in the present invention, methyllobacterium Aquaticum HKMC-1 ( Methylobacterium In addition aquaticum HKMC-1) (KCCM11325P) is coated with a biofilm, the microorganisms in addition to the methyl to coat the biofilm tumefaciens Beuraki Atum HKMC-2 (Methylobacterium brachiatum HKMC- 2) (KCCM11326P), a bacterium methyl Solarium Platani HKMC-3 ( Methylobacterium platani HKMC-3) (KCCM11327P) , Acinetobacter Jones you HKMC-4 (Acinetobacter johnsonii HKMC- 4) (KCCM11328P), Bacillus Betnamensis HKMC-5 ( Bacillus vietnamensis HKMC-5)(KCCM11329P), Brevibacillus Inboard car tooth HKMC-6 (Brevibacillus invocatus HKMC- 6) (KCCM11330P), Day Noko kusu blood kusu HKMC-7 (Deinococcus ficus HKMC-7) (KCCM11331P), grapefruits Sonia Solid HKMC-8 (Leifsonia soli HKMC- 8) (KCCM11332P), Pseudomonas nitro Lowry dew sense HKMC-9 (Pseudomonas nitroreducens HKMC- 9) (KCCM11333P) and Sphingomonas Aqua subtilis HKMC-10 (sphingomonas aquatilis HKMC- 10) (KCCM11334P) and methyl tumefaciens Komagata HKMC-11 ( Methylobacterium komagatae HKMC-11) (KCCM11335P) can provide an odorless Evacore that does not cause odor in an air conditioner, characterized in that the coating is additionally included and coated with one or more microorganisms selected from the group consisting of the above. It is not limited to microorganisms, and any microorganism capable of exhibiting an odorless effect through a combination of various microorganisms is possible.

Eva core is characterized in that the material is aluminum or aluminum alloy, and the eva core is manufactured using aluminum with antibacterial treatment on the eva core or an alloy material without aluminum antibacterial treatment. However, the material of EVA core is not limited to aluminum or aluminum alloy, and in general, EVA core can be used as a material and all metals with excellent corrosion resistance, such as copper (copper), in addition to aluminum, can be used as a material, and can also be used as an alloy manufacturing. The back can be used by connecting a heat exchanger to a Peltier element, and any of the same and similar structures that facilitate heat exchange can be used as materials.

Methylobacterium of the present invention Aquaticum HKMC-1 ( Methylobacterium aquaticum HKMC-1) Biofilm inhibits the reproduction of odor-causing microorganisms, so it does not cause odor.

When the biofilm is coated on the surface of Evacore, the deposition and propagation of bacteria and molds that cause odors are prevented. That is, when a biofilm is coated on the surface of Evacore in advance using specific microorganisms, etc., other types of bacteria and fungi living in an environment different from these specific microorganisms cannot be deposited and propagated on the Evacore surface.

The odorless biofilm is not limited to being composed of only one type of microorganism, and the methylobacterium Aquaticum HKMC-1 ( Methylobacterium aquaticum HKMC-1) (KCCM11325P) the EVA core coated with a bio-film is a methyl in addition to the above microorganisms tumefaciens Brachiatum HKMC-2 ( Methylobacterium brachiatum HKMC-2) (KCCM11326P) , a bacterium methyl Solarium Platani HKMC-3 ( Methylobacterium platani HKMC-3) (KCCM11327P), Acinetobacter Johnsney HKMC-4 ( Acinetobacter johnsonii HKMC-4)(KCCM11328P), Bacillus Betnamensis HKMC-5 ( Bacillus vietnamensis HKMC-5)(KCCM11329P), Brevibacillus Invoke Car Bluetooth HKMC-6 (Brevibacillus invocatus HKMC-6) (KCCM11330P) , Day Noko kusu blood kusu HKMC-7 (Deinococcus ficus HKMC-7) (KCCM11331P), grapefruits Sonia Soli HKMC-8 ( Leifsonia soli HKMC-8) (KCCM11332P), Pseudomonas nitro Lowry dew sense HKMC-9 (Pseudomonas nitroreducens HKMC- 9) (KCCM11333P) and Sphingomonas Aqua Antilles HKMC-10 (sphingomonas aquatilis HKMC-10) (KCCM11334P) and methyl tumefaciens Komagata HKMC-11 ( Methylobacterium The biofilm coated with one or more microorganisms selected from the group consisting of komagatae HKMC-11)(KCCM11335P) additionally suppresses the propagation of odor-causing microorganisms and does not cause odor.

According to another aspect of the present invention, the present invention

(i) Methylobacterium on the surface of Evacore Aquaticum HKMC-1 ( Methylobacterium aquaticum HKMC-1) applying a medium beneficial to reproduction;

(ii) a methyl on the surface of the medium is applied tumefaciens Affixing the aqua tikum HKMC-1 (Methylobacterium aquaticum HKMC- 1) (KCCM11325P); and

(iii) the methylobacterium Aquaticum HKMC-1 ( Methylobacterium a cultivation step of breeding aquaticum HKMC-1) (KCCM11325P) to form a biofilm;

It provides an odorless EVA core manufacturing method that does not cause odor in an air conditioner comprising a.

In the step (i), the step of applying a medium advantageous for propagation is a step that can be omitted as long as the microorganisms can be naturally deposited as a means for facilitating the deposition of microorganisms.

In step (ii), methyllobacterium Aquaticum HKMC-1 ( Methylobacterium In addition aquaticum HKMC-1) (KCCM11325P) bacterium with methyl Solarium Beuraki Atum HKMC-2 (Methylobacterium brachiatum HKMC- 2) (KCCM11326P), a bacterium methyl Solarium Platani HKMC-3 ( Methylobacterium platani HKMC-3) (KCCM11327P) , Acinetobacter Jones you HKMC-4 (Acinetobacter johnsonii HKMC- 4) (KCCM11328P), Bacillus Betnamensis HKMC-5 ( Bacillus vietnamensis HKMC-5)(KCCM11329P), Brevibacillus Inboard car tooth HKMC-6 (Brevibacillus invocatus HKMC- 6) (KCCM11330P), Day Noko kusu blood kusu HKMC-7 (Deinococcus ficus HKMC-7) (KCCM11331P), grapefruits Sonia Solid HKMC-8 (Leifsonia soli HKMC- 8) (KCCM11332P), Pseudomonas nitro Lowry dew sense HKMC-9 (Pseudomonas nitroreducens HKMC- 9) (KCCM11333P) and Sphingomonas Aqua subtilis HKMC-10 (sphingomonas aquatilis HKMC- 10) (KCCM11334P) and methyl tumefaciens Komagata HKMC-11 ( Methylobacterium Komagatae HKMC-11)(KCCM11335P) can be coated with a biofilm to additionally coat one or two or more microorganisms selected from the group consisting of odorless microorganisms to produce an odorless biofilm, which allows the reproduction of odorous microorganisms. Suppresses

According to a preferred embodiment of the present invention, the surface of the Evacore may be made of aluminum or an aluminum alloy, and the aluminum or aluminum alloy is prepared by inoculating odorless microorganisms after preparing the Evacore subjected to antibacterial treatment to form a biofilm. Can be manufactured. Also Aluminum or aluminum alloy can be produced through microbial inoculation without antibacterial treatment.

Hereinafter, identification of odorless microorganisms in Evacore according to a preferred embodiment of the present invention and an odorless biofilm for an air conditioner using the same will be described in detail with reference to the accompanying drawings.

One. Example 1: Securing used cars with odor odor

No. Car type sample type One Vehicle type A Eva Core 2 Vehicle type B Eva Core 3 Vehicle type C Eva Core 4 Vehicle type D Eva Core 5 Car model E Eva Core

The present inventors attempted to sample the evacore specimens by securing five used cars with a smell of odor, and removing the eva core mounted on each vehicle type A to vehicle type E.

2. Example 2 : Eva Core Specimen sampling

The EVA core samples obtained from the odor used cars A to E were stored refrigerated at 4° C. before use, and were sealed and stored in a polyethylene bag. In order to separate and cultivate microorganisms, 5 g of fin samples were collected from each evacore, including the front part and the back part, using a sterilized long nose plier, and then mixed and used.

3. Example 3: From Evacore Microbial Tally process

A procedure and method for desorbing microorganisms from Evacore through the following procedure have been described.

① Mix the sample extracted from Evacore and put it into a mixer.

② Add sterilized 1× Phosphate buffed saline (PBS) to 200ml mixer.

③ Mix the mixed sample and PBS for 30 seconds.

④ Put the mixer on ice for 1 minute.

⑤ Repeat the ③-④ step two more times.

⑥ Centrifuge the suspension at 13000rpm 4℃ for 3 minutes.

⑦ Take only the supernatant and transfer it to a new tube.

⑧ Wet a sterilized cotton swab in the supernatant and wipe the surface of the Evacore sampled several times.

⑨ Put only the head in the supernatant and votexing the wiped cotton swab.

⑩ Mix the sediment obtained in step ⑥ with the mixture ⑨ and use it as an inoculum stock solution.

Microorganisms were separated by physical desorption of the Eva cores installed in vehicle types A through E in the above steps ① to ⑩.

4. Example 4: Isolation and culture of bacteria

Bacteria in air conditioners are generally separated from aerobic heterotrophic bacteria, called general bacteria, through heterotrophic plate culture. Complex nutrient media used for separation of common bacteria are PTYG agar medium and R2A agar medium. PTYG agar medium is Peptone 0.25g (Difco), Triptone 0.25g (Difco), Yeast extract 0.5g (Difco), Glucose 0.5g (Difco), MgSO ₄ 30mg (Sigma), CaCl ₂ 3mg (Sigma), Bacto agar 15g (Difco) was added to 980 ml of distilled water, adjusted to pH 7.0, and autoclaved at 121°C for 15 minutes. R2A arar medium is Yeast extract 0.5g (Difco), Proteose peptone No.3 0.5g (Difco), Casamino acids 0.5g (Difco), Dextrose 0.5g (Difco), Soluble starch 0.5g (Difco), Sodium pyruvate 0.3g (Difco), Dipotassium sulfate 0.3g (Difco), Magnesium sulfate 0.05g (Difco), and Bacto agar 15g (Difco) were added to 980ml of distilled water, adjusted to pH 7.2, and autoclaved at 121°C for 15 minutes. For the isolation of non-dominant common bacteria, three types of antibiotics were used (Table 2), and each antibiotic was filtered at a concentration of 100 ppm and inoculated when the medium temperature reached 50°C to prepare an antibiotic medium.

No. Antibiotic line manufacturer One Kanamycin Aminoglycoside Sigma 2 Ampicillin beta-lactam Sigma 3 Chloramphenicol Chloramphenicol Sigma

5. Example 5: Separation and cultivation of fungi (fungi)

Separate culture of air conditioner fungi (fungi) is separated through aerobic plate culture in nutrient medium. Two types of media used for the isolation culture of fungi (fungi) were Potato dextrose agar medium and Malt extract agar medium. Potato dextrose agar medium was put into 980 ml of distilled water, 4g of Potato starch (Difco), 20g of Dextrose (Difco), and 15g of Bacto agar (Difco), adjusted to pH 5.1, and autoclaved at 121°C for 15 minutes. Malt extract agar medium was 20g of Malt extract (Difco) and 15g of Bacto agar (Difco) in 980ml of distilled water, pH was adjusted to 5.0, and autoclaved at 121℃ for 15 minutes.

A 90mm×15mm petri dish was used to separate and cultivate the fungi, and a 60mm×15mm petri dish was used to separate and cultivate the cultured fungi.

6. Example 6: Isolation of the EVA core dominant in the entire culture medium cultured microorganism strain

In order to separate and culture the dominant strain, first, various dominant strains must be selected through a morphological approach such as dilution ratio, colony color, size, and shape, so the dominant strain is separated and cultured according to the following procedure.

① Separate fungi and bacteria from separately cultured medium.

② Various bacteria with different morphology are inoculated into a complex medium by using a loop and purified.

③ Select the medium with the best growth among the inoculated medium and subculture.

④ The fungus is inoculated on a complex medium after separating the tip of the mycelium with a scalpel.

⑤ The fungal strain is also subcultured by selecting the best growth medium among the inoculated medium.

7. Example 7: Eva Core Advantage Analysis of the genetic characteristics of bacteria

Fingerprints investigation through REP-PCR pattern analysis

REP-PCR is a molecular biological method that analyzes the structure of bacterial chromosomes, and is a fingerprinting method that can distinguish and identify each bacterial strain from other bacteria. In order to perform REP-PCR, genetic characteristics were analyzed according to each of the following procedures.

(One) Cell lysis step

① Put 2.5µl of Lyse-N-Go PCR Reagent (Thermo) into a PCR tube.

② Pick the colony from the clean bench with a pipette, put it in the upper tube, and pipet it. Take care not to make the solution slightly cloudy.

③ Cultivate it in a PCR machine according to the manufacturer's instructions.

④ Repeat the cycle according to the Lysis program described in Table 3 below, and leave it as it is without turning it off when it reaches 80℃ in the 9th cycle.

Cycle Temperature(℃) Time(seconds) One 65 30 2 8 30 3 65 90 4 97 180 5 8 60 6 65 180 7 97 60 8 65 60 9 80 hold

(2) PCR reaction

A reaction mixture was prepared by mixing the components required for the PCR reaction described in Table 4 below according to the required amount, and using this, as shown in Table 5, a pre-denaturation step (pre-denaturation) at 94° C. for 7 minutes, a denaturation step ( The PCR amplification process was performed by repeating denaturation, cooling, and extension for 1 minute at 92°C for 1 minute, 51.5°C for 1 minute at annealing, and 8 minutes at 65°C at the extension step for 8 minutes.

① dNTP (2.5mM each) 12.5 μl ② Gitschier buffer 5.0 μl ③ DMSO (100%) 2.5 μl ④ Autoclaved 3 ^o DW 0.3 μl ⑤ BOXA1R primer (50 pmole/µl) 1.0 μl 5'CTACGGCAAGGCGACGCTGACG ⑥ BSA (10mg/ml) 0.4 μl ⑦ Bacterial DNA 2.5µl ⑧ Taq polymerase(Roche) (5 U/ul) 0.8 μl

step 1 93℃ 7min step 2 92℃ 1min step 3 51.5℃ 1min step 4 65℃ 8min step 2,3,4: additional 33 cycles step 6 65℃ 16min step 7 4℃

(3) Gel electrophoresis

DNA fragments amplified by each PCR were taken, and a 1.2-1.5% agarose gel added with EtBr was used, and 6x dye was mixed with the sample in a ratio of 1:5 to load as much as possible. Since most PCR products are between 100 and 1000 bp, load a 100 bp ladder together and electrophoresis as slowly as possible (50 V) so that the middle of bromophenol blue and xylene cyanol dye goes to the middle of the entire gel. Strains with the same DNA pattern on the gel are considered to be the same strain.

(4) Air conditioner Advantage 16S of bacteria rRNA Identification through genetic analysis

The 16S rRNA (ribosomal ribonucleic acid) gene is used to identify the genetic classification of bacteria, and can be identified at the genus and species level of the classified bacteria through REP-PCR. 16S rRNA is an RNA that forms a ribosome by interacting with various proteins, and its complete sequence or nucleotide sequence for the list of oligonucleotides has been identified in more than 2000 bacteria, so it is based on the genetic similarity of 16S rRNA. Thus, bacteria can be divided into several major groups. Since the rate of change of the 16S rRNA gene sequence is significantly less than that of other gene sequences in most genomes, it is recognized that the degree of 16S rRNA sequence similarity reflects the phylogenetic distance between organisms. The method of identifying microorganisms according to similarity by analyzing the base sequence of the 16S rRNA gene fragment has been used as a representative identification method for identifying microorganisms, particularly industrially useful microorganisms, along with the above-described fatty acid analysis and carbohydrate magnetization ability analysis.

<16S rRNA PCR>

PCR condtions (Total 50 µl): As shown in Table 6 below, the remaining solutions excluding DNA and Taq were mixed as required, and 44.5 µl was added to the above lysis solution. Thereafter, as shown in Table 7, the pre-denaturation step (pre-denaturation) at 94°C for 5 minutes, denaturation step (denaturation) at 94°C for 1 minute, cooling step (annealing) at 55°C for 1 minute, extension step (extension) ) At 72° C. for 1 minute and 30 seconds, the steps of denaturation, cooling, and extension were performed 29 times to perform PCR amplification process.

Autoclaved 3 ^o DW 22µl 10xbuffer (Roche) 5µl dNTP (Roche, 2.5mM) 5µl DMSO 5µl BSA (10mg/ml) 2.5µl 27mf (20pmole/µl) 2.5µl 1492r (20pmole/µl) 2.5µl DNA 5µl Taq (Roche) 0.5 μl

step 1 94℃ 5min step 2 94℃ 1min step 3 55℃ 1min step 4 72℃ 1min 30sec Go to step 2: additional 29 cycles step 6 72℃ 10min step 7 4℃ hold

(5) PCR purification

The product amplified through 16S-rRNA PCR was purified according to the following procedure using a Qiaquick PCR purifcation kit.

① Put 5 times the PB buffer of the PCR product.

② Dispense the mixed solution into the QIAquick column.

③ To bind DNA, centrifuge for 1 minute and then remove the mixed solution.

④ For washing, put 750µl of PE buffer into the QIAquick column, centrifuge for 1 minute, and remove the mixed solution.

⑤ Centrifuge again for 1 minute.

⑥ Move the QIAquick column to a new tube.

⑦ To extract DNA, add 30µl of EB buffer and leave for 1 minute.

⑧ Centrifuge for 1 minute to collect the DNA dissolved in EB in the tube.

(6) Name of each isolated microorganism and characteristics of microorganism

<Microorganism 1>

1. Name of microorganism: HKMC-1

Genus name: Methylobacterium

Species name: aquaticum

2. Restoration conditions

end. Restorer

(1) Composition: PTYG medium (Peptone 0.25 g per 1 L, Triptone 0.25 g, Yeast extract 0.5 g, Glucose 0.5 g, MgSO ₄ 30 mg, CaCl ₂ 3 mg) or R2A medium.

(2) pH: 7.0

(3) Sterilization conditions: 20 minutes at 121℃

I. Incubation at temperature (℃) 28℃ for 7 days

3. Badge

(1) Composition: PTYG medium (Peptone 0.25 g per 1 L, Triptone 0.25 g, Yeast extract 0.5 g, Glucose 0.5 g, MgSO ₄ 30 mg, CaCl ₂ 3 mg) or R2A medium.

(2) pH: 7.0

(3) Sterilization conditions: 20 minutes at 121℃

4. Culture conditions

end. Aerobic, anaerobic: aerobic

I. Temperature (℃) 28℃

All. Shaking, stationary (liquid, solid) can be used without distinction

5. Preservation conditions

Temperature (℃) -70℃

6. 16S rRNA gene sequence

SEQ ID NO: 1

<Microorganism 2>

1. Name of microorganism: HKMC-2

Genus name: Methylobacterium

Species name: brachiatum

2. Restoration conditions

end. Restorer

(1) Composition: PTYG medium (Peptone 0.25 g per 1 L, Triptone 0.25 g, Yeast extract 0.5 g, Glucose 0.5 g, MgSO ₄ 30 mg, CaCl ₂ 3 mg) or R2A medium.

(2) pH: 7.0

(3) Sterilization conditions: 20 minutes at 121℃

I. Incubation at temperature (℃) 28℃ for 7 days

3. Badge

(1) Composition: PTYG medium (Peptone 0.25 g per 1 L, Triptone 0.25 g, Yeast extract 0.5 g, Glucose 0.5 g, MgSO ₄ 30 mg, CaCl ₂ 3 mg) or R2A medium.

(2) pH: 7.0

(3) Sterilization conditions: 20 minutes at 121℃

4. Culture conditions

end. Aerobic, anaerobic: aerobic

I. Temperature (℃) 28℃

All. Shaking, stationary (liquid, solid) can be used without distinction

5. Preservation conditions

Temperature (℃) -70℃

6. 16S rRNA gene sequence

SEQ ID NO: 2

<Microorganism 3>

1. Name of microorganism: HKMC-3

Genus name: Methylobacterium

Species name: platani

2. Restoration conditions

end. Restorer

(1) Composition: PTYG medium (Peptone 0.25 g per 1 L, Triptone 0.25 g, Yeast extract 0.5 g, Glucose 0.5 g, MgSO ₄ 30 mg, CaCl ₂ 3 mg) or R2A medium.

(2) pH: 7.0

* (3) Sterilization conditions: 20 minutes at 121℃

I. Incubation at temperature (℃) 28℃ for 7 days

3. Badge

(1) Composition: PTYG medium (Peptone 0.25 g per 1 L, Triptone 0.25 g, Yeast extract 0.5 g, Glucose 0.5 g, MgSO ₄ 30 mg, CaCl ₂ 3 mg) or R2A medium.

(2) pH: 7.0

(3) Sterilization conditions: 20 minutes at 121℃

4. Culture conditions

end. Aerobic, anaerobic: aerobic

I. Temperature (℃) 28℃

All. Shaking, stationary (liquid, solid) can be used without distinction

5. Preservation conditions

Temperature (℃) -70℃

6. 16S rRNA gene sequence

SEQ ID NO: 3

<Microorganism 4>

1. Name of microorganism: HKMC-4

Genus name: Acinetobacter

Species name: johnsonii

2. Restoration conditions

end. Restorer

(1) Composition: PTYG medium (Peptone 0.25 g per 1 L, Triptone 0.25 g, Yeast extract 0.5 g, Glucose 0.5 g, MgSO ₄ 30 mg, CaCl ₂ 3 mg) or R2A medium.

(2) pH: 7.0

(3) Sterilization conditions: 20 minutes at 121℃

I. Incubation at temperature (℃) 28℃ for 7 days

3. Badge

(1) Composition: PTYG medium (Peptone 0.25 g per 1 L, Triptone 0.25 g, Yeast extract 0.5 g, Glucose 0.5 g, MgSO ₄ 30 mg, CaCl ₂ 3 mg) or R2A medium.

(2) pH: 7.0

(3) Sterilization conditions: 20 minutes at 121℃

4. Culture conditions

end. Aerobic, anaerobic: aerobic

I. Temperature (℃) 28℃

All. Shaking, stationary (liquid, solid) can be used without distinction

5. Preservation conditions

Temperature (℃) -70℃

6. 16S rRNA gene sequence

SEQ ID NO: 4

<Microorganism 5>

1. Name of microorganism: HKMC-5

Genus: Bacillus

Species name: vietnamensis

2. Restoration conditions

end. Restorer

(1) Composition: PTYG medium (Peptone 0.25 g per 1 L, Triptone 0.25 g, Yeast extract 0.5 g, Glucose 0.5 g, MgSO ₄ 30 mg, CaCl ₂ 3 mg) or R2A medium.

(2) pH: 7.0

(3) Sterilization conditions: 20 minutes at 121℃

I. Incubation at temperature (℃) 28℃ for 7 days

3. Badge

(1) Composition: PTYG medium (Peptone 0.25 g per 1 L, Triptone 0.25 g, Yeast extract 0.5 g, Glucose 0.5 g, MgSO ₄ 30 mg, CaCl ₂ 3 mg) or R2A medium.

(2) pH: 7.0

(3) Sterilization conditions: 20 minutes at 121℃

4. Culture conditions

end. Aerobic, anaerobic: aerobic

I. Temperature (℃) 28℃

All. Shaking, stationary (liquid, solid) can be used without distinction

5. Preservation conditions

Temperature (℃) -70℃

6. 16S rRNA gene sequence

SEQ ID NO: 5

<Microorganism 6>

1. Name of microorganism: HKMC-6

Genus name: Brevibacillus

Species name: invocatus

2. Restoration conditions

end. Restorer

(1) Composition: PTYG medium (Peptone 0.25 g per 1 L, Triptone 0.25 g, Yeast extract 0.5 g, Glucose 0.5 g, MgSO ₄ 30 mg, CaCl ₂ 3 mg) or R2A medium.

(2) pH: 7.0

(3) Sterilization conditions: 20 minutes at 121℃

I. Incubation at temperature (℃) 28℃ for 7 days

3. Badge

(1) Composition: PTYG medium (Peptone 0.25 g per 1 L, Triptone 0.25 g, Yeast extract 0.5 g, Glucose 0.5 g, MgSO ₄ 30 mg, CaCl ₂ 3 mg) or R2A medium.

(2) pH: 7.0

(3) Sterilization conditions: 20 minutes at 121℃

4. Culture conditions

end. Aerobic, anaerobic: aerobic

I. Temperature (℃) 28℃

All. Shaking, stationary (liquid, solid) can be used without distinction

5. Preservation conditions

Temperature (℃) -70℃

6. 16S rRNA gene sequence

SEQ ID NO: 6

<Microorganism 7>

1. Name of microorganism: HKMC-7

Genus name: Deinococcus

Species name: ficus

2. Restoration conditions

end. Restorer

(1) Composition: PTYG medium (Peptone 0.25 g per 1 L, Triptone 0.25 g, Yeast extract 0.5 g, Glucose 0.5 g, MgSO ₄ 30 mg, CaCl ₂ 3 mg) or R2A medium.

(2) pH: 7.0

(3) Sterilization conditions: 20 minutes at 121℃

I. Incubation at temperature (℃) 28℃ for 7 days

3. Badge

(1) Composition: PTYG medium (Peptone 0.25 g per 1 L, Triptone 0.25 g, Yeast extract 0.5 g, Glucose 0.5 g, MgSO ₄ 30 mg, CaCl ₂ 3 mg) or R2A medium.

(2) pH: 7.0

(3) Sterilization conditions: 20 minutes at 121℃

4. Culture conditions

end. Aerobic, anaerobic: aerobic

I. Temperature (℃) 28℃

All. Shaking, stationary (liquid, solid) can be used without distinction

5. Preservation conditions

Temperature (℃) -70℃

6. 16S rRNA gene sequence

SEQ ID NO: 7

<Microorganism 8>

1. Name of microorganism: HKMC-8

Genus name: Leifsonia

Species name: soli

2. Restoration conditions

end. Restorer

(1) Composition: PTYG medium (Peptone 0.25 g per 1 L, Triptone 0.25 g, Yeast extract 0.5 g, Glucose 0.5 g, MgSO ₄ 30 mg, CaCl ₂ 3 mg) or R2A medium.

(2) pH: 7.0

(3) Sterilization conditions: 20 minutes at 121℃

I. Incubation at temperature (℃) 28℃ for 7 days

3. Badge

(1) Composition: PTYG medium (Peptone 0.25 g per 1 L, Triptone 0.25 g, Yeast extract 0.5 g, Glucose 0.5 g, MgSO ₄ 30 mg, CaCl ₂ 3 mg) or R2A medium.

(2) pH: 7.0

(3) Sterilization conditions: 20 minutes at 121℃

4. Culture conditions

end. Aerobic, anaerobic: aerobic

I. Temperature (℃) 28℃

All. Shaking, stationary (liquid, solid) can be used without distinction

5. Preservation conditions

Temperature (℃) -70℃

6. 16S rRNA gene sequence

SEQ ID NO: 8

<Microorganism 9>

1. Name of microorganism: HKMC-9

Genus name: Pseudomonas

Species name: nitroreducens

2. Restoration conditions

end. Restorer

(1) Composition: PTYG medium (Peptone 0.25 g per 1 L, Triptone 0.25 g, Yeast extract 0.5 g, Glucose 0.5 g, MgSO ₄ 30 mg, CaCl ₂ 3 mg) or R2A medium.

(2) pH: 7.0

(3) Sterilization conditions: 20 minutes at 121℃

I. Incubation at temperature (℃) 28℃ for 7 days

3. Badge

(1) Composition: PTYG medium (Peptone 0.25 g per 1 L, Triptone 0.25 g, Yeast extract 0.5 g, Glucose 0.5 g, MgSO ₄ 30 mg, CaCl ₂ 3 mg) or R2A medium.

(2) pH: 7.0

(3) Sterilization conditions: 20 minutes at 121℃

4. Culture conditions

end. Aerobic, anaerobic: aerobic

I. Temperature (℃) 28℃

All. Shaking, stationary (liquid, solid) can be used without distinction

5. Preservation conditions

Temperature (℃) -70℃

6. 16S rRNA gene sequence

SEQ ID NO: 9

<Microorganism 10>

1. Name of microorganism: HKMC-10

Genus name: Sphingomonas

Species name: aquatilis

2. Restoration conditions

end. Restorer

(1) Composition: PTYG medium (Peptone 0.25 g per 1 L, Triptone 0.25 g, Yeast extract 0.5 g, Glucose 0.5 g, MgSO ₄ 30 mg, CaCl ₂ 3 mg) or R2A medium.

(2) pH: 7.0

(3) Sterilization conditions: 20 minutes at 121℃

I. Incubation at temperature (℃) 28℃ for 7 days

3. Badge

(1) Composition: PTYG medium (Peptone 0.25 g per 1 L, Triptone 0.25 g, Yeast extract 0.5 g, Glucose 0.5 g, MgSO ₄ 30 mg, CaCl ₂ 3 mg) or R2A medium.

(2) pH: 7.0

(3) Sterilization conditions: 20 minutes at 121℃

4. Culture conditions

end. Aerobic, anaerobic: aerobic

I. Temperature (℃) 28℃

All. Shaking, stationary (liquid, solid) can be used without distinction

5. Preservation conditions

Temperature (℃) -70℃

6. 16S rRNA gene sequence

SEQ ID NO: 10

<Microorganism 11>

1. Name of microorganism: HKMC-11

Genus name: Methylobacterium

Species name: komagatae

2. Restoration conditions

end. Restorer

(1) Composition: PTYG medium (Peptone 0.25 g per 1 L, Triptone 0.25 g, Yeast extract 0.5 g, Glucose 0.5 g, MgSO ₄ 30 mg, CaCl ₂ 3 mg) or R2A medium.

(2) pH: 7.0

(3) Sterilization conditions: 20 minutes at 121℃

I. Incubation at temperature (℃) 28℃ for 7 days

3. Badge

(1) Composition: PTYG medium (Peptone 0.25 g per 1 L, Triptone 0.25 g, Yeast extract 0.5 g, Glucose 0.5 g, MgSO ₄ 30 mg, CaCl ₂ 3 mg) or R2A medium.

(2) pH: 7.0

(3) Sterilization conditions: 20 minutes at 121℃

4. Culture conditions

end. Aerobic, anaerobic: aerobic

I. Temperature (℃) 28℃

All. Shaking, stationary (liquid, solid) can be used without distinction

5. Preservation conditions

Temperature (℃) -70℃

6. 16S rRNA gene sequence

SEQ ID NO: 11

8. Example 8: Sensory evaluation of isolated microorganisms

(One) In nutrient medium Sensory evaluation

In order to analyze the organoleptic properties of the microorganisms identified in Example 7 above, evaluation was performed after culturing at 28°C for 7 days in a nutrient medium from which the microorganisms were separated. The following describes the process of culturing the bacteria in a nutrient medium.

① Inoculate purely separated and cultured microorganisms into liquid nutrient medium.

② Inoculate the inoculated medium at 28℃ for 5-7 days.

③ In a solid nutrient medium, inoculate 100 µl of cells cultured in a liquid medium.

④ Spread the inoculated cells evenly using a spreader.

⑤ Seal the petri dish and incubate it at 28℃ for 10 days.

(2) Sensory evaluation in aluminum pin medium

The square-sized aluminum pins were sterilized, and they were dipping into dust and nutrient medium. Thereafter, bacteria were inoculated and cultured in the same manner as in steps ②-④ in the nutrient medium, and then evaluated, and the sensory evaluation results are described in Table 8 below.

① Aluminum pin treated with antibacterial agent: It is a coating of Evacore finished product that can be purchased sufficiently in the market that is mass-produced with an antibacterial coating on aluminum, the main material of Evacore.

② Aluminum pins treated with hydrophilic coating without antimicrobial treatment: These are aluminum pins that have undergone only the hydrophilic process of Evacore's coating process. Usually, the hydrophilic process and the antibacterial process are performed at the same time. It is specially designed to compare antimicrobial coated pins with non-antibacterial coated pins. Eva core is manufactured with aluminum pins to reduce weight, but it can be manufactured with various metals such as copper and steel.

NO. Antibacterial pin Antibacterial agent pin 16S rRNA One Odorless Odorless Methylobacterium aquaticum HKMC -One 2 Odorless Odorless Methylobacterium brachiatum HKMC-2 3 Odorless Odorless Methylobacterium platani HKMC-3 4 Odorless Odorless Acinetobacter johnsonii HKMC-4 5 Odorless Odorless Bacillus vietnamensis HKMC-5 6 Odorless Odorless Brevibacillus invocatus HKMC-6 7 Odorless Odorless Deinococcus ficus HKMC-7 8 Odorless Odorless Leifsonia soli HKMC-8 9 Odorless Odorless Pseudomonas nitroreducens HKMC-9 10 Odorless Odorless Sphingomonas aquatilis HKMC-10 11 Odorless Odorless Methylobacterium komagatae HKMC-11

The result of inoculating and culturing the 11 kinds of microorganisms in both aluminum antibacterial and non-antibacterial pins showed odorless results. The field of application of the odorless microorganisms can be sufficiently used for the purpose of preventing odors caused by microorganisms living in the eva core of an air conditioner regardless of the metal material.

Korea Microorganism Conservation Center (overseas) KCCM11325P 20121114 Korea Microorganism Conservation Center (overseas) KCCM11326P 20121114 Korea Microorganism Conservation Center (overseas) KCCM11327P 20121114 Korea Microorganism Conservation Center (overseas) KCCM11328P 20121114 Korea Microorganism Conservation Center (overseas) KCCM11329P 20121114 Korea Microorganism Conservation Center (overseas) KCCM11330P 20121114 Korea Microorganism Conservation Center (overseas) KCCM11331P 20121114 Korea Microorganism Conservation Center (overseas) KCCM11332P 20121114 Korea Microorganism Conservation Center (overseas) KCCM11333P 20121114 Korea Microorganism Conservation Center (overseas) KCCM11334P 20121114 Korea Microorganism Conservation Center (overseas) KCCM11335P 20121114

<110> Hyundai Motor Company <120> Methylobacterium brachiatum for Odorless Bioflim-Coated Evaporator Core and uses thereof <160> 11 <170> KopatentIn 1.71 <210> 1 <211> 1411 <212> DNA <213> Methylobacterium aquaticum HKMC-1 <220> <221> rRNA <222> (1)..(1411) <223> 16S rRNA <400> 1 cagagcgaac gctggcggca ggcttaacac atgcaagtcg agcgggccct tcggggtcag 60 cggcagacgg gtgagtaacg cgtgggaacg tgcccttcgg ttcggaataa ctcagggaaa 120 cttgagctaa taccggatac gcccttatgg ggaaaggctt gactgccgaa ggatcggccc 180 gcgtctgatt agctagttgg tgaggttacg gctcaccaag gcgacgatca gtagctggtc 240 tgagaggatg atcagccaca ctgggactga gacacggccc agactcctac gggaggcagc 300 agtggggaat attggacaat gggggcaacc ctgatccagc catgccgcgt gagtgatgac 360 ggccttaggg ttgtaaagct ctttctccgg gacgataatg acggtaccgg aggaataagc 420 cccggctaac ttcgtgccag cagccgcggt aatacgaagg gggctagcgt tgctcggaat 480 cactgggcgt aaagggcgcg taggcggctg atttagtcga gggtgaaagc ccgtggctca 540 accacggaat ggccttcgat actggttggc ttgagaccgg aagaggacag cggaactgcg 600 agtgtagagg tgaaattcgt agatattcgc aagaacacca gtggcgaagg cggctgtctg 660 gtccggttct gacgctgagg cgcgaaagcg tggggagcaa acaggattag ataccctggt 720 agtccacgct gtaaacgatg aatgctagcc gttggggtgc atgcacctca gtggcgccgc 780 taacgcatta agcattccgc ctggggagta cggtcgcaag attaaaactc aaaggaattg 840 acgggggccc gcacaagcgg tggagcatgt ggtttaattc gaagcaacgc gcagaacctt 900 accatccctt gacatggcgt gttatccgga gagatccggg gtccccttcg ggggcgcgca 960 cacaggtgct gcatggctgt cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa 1020 cgagcgcaac ccacgtccct agttgccatc atttggttgg gcactctggg gagactgccg 1080 gtgataagcc gcgaggaagg tgtggatgac gtcaagtcct catggccctt acgggatggg 1140 ctacacacgt gctacaatgg cggtgacaat gggcagcgaa ggggcgacct ggagcgaatc 1200 cccaaaagcc gtctcagttc ggattgcact ctgcaactcg ggtgcatgaa ggcggaatcg 1260 ctagtaatcg tggatcagca cgccacggtg aatacgttcc cgggccttgt acacaccgcc 1320 cgtcacacca tgggagttgg tcttacccga cggcgctgcg ccaaccgcaa ggaggcaggc 1380 gaccacggta gggtcagcga ctggggtgaa g 1411 <210> 2 <211> 1416 <212> DNA <213> Methylobacterium brachiatum HKMC-2 <220> <221> rRNA <222> (1)..(1416) <223> 16S rRNA <400> 2 ggctgagaga gaacgggggg ggcaggttta acacatacaa gtagagggga cctttggggg 60 tcagcggcgg acgggtgagt aacgcgtggg aacgtgccct ctggttcgga ataactcagg 120 gaaacttgag ctaataccgg atacgccctt ttggggaaag gcttgctgcc ggaggatcgg 180 cccgcgtctg attagctagt tggtgaggta acggctcacc aaggcgacga tcagtagctg 240 gtctgagagg atgatcagcc acactgggac tgagacacgg cccagactcc tacgggaggc 300 agcagtgggg aatattggac aatgggcgca agcctgatcc agccatgccg cgtgagtgat 360 gaaggcctta gggttgtaaa gctcttttat ccgggacgat aatgacggta ccggaggaat 420 aagccccggc taacttcgtg ccagcagccg cggtaatacg aagggggcta gcgttgctcg 480 gaatcactgg gcgtaaaggg cgcgtaggcg gcgttttaag tcgggggtga aagcctgtgg 540 ctcaaccaca gaatggcctt cgatactggg acgcttgagt atggtagagg ttggtggaac 600 tgcgagtgta gaggtgaaat tcgtagatat tcgcaagaac accggtggcg aaggcggcca 660 actggaccat tactgacgct gaggcgcgaa agcgtgggga gcaaacagga ttagataccc 720 tggtagtcca cgccgtaaac gatgaatgcc agctgttggg gtgcttgcac ctcagtagcg 780 cagctaacgc tttgagcatt ccgcctgggg agtacggtcg caagattaaa actcaaagga 840 attgacgggg gcccgcacaa gcggtggagc atgtggttta attcgaagca acgcgcagaa 900 ccttaccatc ctttgacatg gcgtgttatg gggagagatt cccagtcctc ttcggaggcg 960 cgcacacagg tgctgcatgg ctgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc 1020 gcaacgagcg caacccacgt ccttagttgc catcattcag ttgggcactc tagggagact 1080 gccggtgata agccgcgagg aaggtgtgga tgacgtcaag tcctcatggc ccttacggga 1140 tgggctacac acgtgctaca atggcggtga cagtgggacg cgaaggggcg acctggagca 1200 aatccccaaa agccgtctca gttcggattg cactctgcaa ctcgggtgca tgaaggcgga 1260 atcgctagta atcgtggatc agcatgccac ggtgaatacg ttcccgggcc ttgtacacac 1320 cgcccgtcac accatgggag ttggtcttac ccgacggcgc tgcgccaacc gcaaggaggc 1380 aggcgaccac ggtagggtca gcgactgggg tgaagt 1416 <210> 3 <211> 1411 <212> DNA <213> Methylobacterium platani HKMC-3 <220> <221> rRNA <222> (1)..(1411) <223> 16S rRNA <400> 3 gaacgaaggc ggcaggctta acacatgcaa gtagagcggg catccctgtg gggttaacgg 60 cagacgggtg agtaaagggt gggaaagtgc cctttggttt ggaataaatt agggaaaact 120 gaagtaattc cggatacgtc cgagagggga aaggtttatc tgccgaagga tcggcccgcg 180 tctgattagc tagttggtga ggttatggct caccaaggcg acgatcagta gctggtctga 240 gaggatgatc agccacactg ggactgagac acggcccaga ctcctacggg aggcagcagt 300 ggggaatatt ggacaatggg ggcaaccctg atccagccat gccgcgtgag tgatgacggc 360 cttagggttg taaagctctt ttctccggga cgataatgac ggtaccggag gaataagccc 420 cggctaactt cgtgccagca gccgcggtaa tacgaagggg gctagcgttg ctcggaatca 480 ctgggcgtaa agggcgcgta ggcggctgat ttagtcgagg gtgaaagccc gtggctcaac 540 cacggaatgg ccttcgatac tgattggctt gagaccggaa gaggacagcg gaactgcgag 600 tgtagaggtg aaattcgtag atattcgcaa gaacaccagt ggcgaaggcg gctgtctggt 660 ccggttctga cgctgaggcg cgaaagcgtg gggagcaaac aggattagat accctggtag 720 tccacgctgt aaacgatgaa tgctagccgt tggggtgcat gcacctcagt ggcgccgcta 780 acgctttaag cattccgcct ggggagtacg gtcgcaagat taaaactcaa aggaattgac 840 gggggcccgc acaagcggtg gagcatgtgg tttaattcga agcaacgcgc agaaccttac 900 catcccttga catggcatgc gagccggaga gatccggtgt tcccttcggg gacgtgcaca 960 caggtgctgc atggctgtcg tcagctcgtg tcgtgagatg ttgggttaag tcccgcaacg 1020 agcgcaaccc acgtcctcag ttgccatcat tcagttgggc actctgggga gactgccggt 1080 gataagccgc gaggaaggtg tggatgacgt caagtcctca tggcccttac gggatgggct 1140 acacacgtgc tacaatggcg gtgacaatgg gcagcgaagg ggcgacctgg agcgaatccc 1200 caaaagccgt ctcagttcgg attgcactct gcaactcggg tgcatgaagg cggaatcgct 1260 agtaatcgtg gatcagcatg ccacggtgaa tacgttcccg ggccttgtac acaccgcccg 1320 tcacaccatg ggagttggtc ttacccgacg gcgctgcgcc aaccgcaagg aggcaggcga 1380 ccacggtagg gtcagcgact ggggtgaagt c 1411 <210> 4 <211> 1472 <212> DNA <213> Acinetobacter johnsonii HKMC-4 <220> <221> rRNA <222> (1)..(1472) <223> 16S rRNA <400> 4 ggctcagatt gaacgctggc ggcaggctta acacatgcaa gtcgagcggg gaaaggtagc 60 ttgctaccta acctagcggc ggacgggtga gtaatgctta ggaatctgcc tattagtggg 120 ggacaacatt ccgaaaggaa tgctaatacc gcatacgccc tacgggggaa agcaggggat 180 cttcggacct tgcgctaata gatgagccta agtcagatta gctagttggt ggggtaaagg 240 cctaccaagg cgacgatctg tagcgggtct gagaggatga tccgccacac tgggactgag 300 acacggccca gactcctacg ggaggcagca gtggggaata ttggacaatg ggcgaaagcc 360 tgatccagcc atgccgcgtg tgtgaagaag gccttttggt tgtaaagcac tttaagcgag 420 gaggaggcta cttagattaa tactctagga tagtggacgt tactcgcaga ataagcaccg 480 gctaactctg tgccagcagc cgcggtaata cagagggtgc gagcgttaat cggatttact 540 gggcgtaaag cgtgcgtagg cggcttctta agtcggatgt gaaatccctg agcttaactt 600 aggaattgca ttcgatactg ggaagctaga gtatgggaga ggatggtaga attccaggtg 660 tagcggtgaa atgcgtagag atctggagga ataccgatgg cgaaggcagc catctggcct 720 aatactgacg ctgaggtacg aaagcatggg gagcaaacag gattagatac cctggtagtc 780 catgccgtaa acgatgtcta ctagccgttg gggcctttga ggctttagtg gcgcagctaa 840 cgcgataagt agaccgcctg gggagtacgg tcgcaagact aaaactcaaa tgaattgacg 900 ggggcccgca caagcggtgg agcatgtggt ttaattcgat gcaacgcgaa gaaccttacc 960 tggtcttgac atagtaagaa ctttccagag atggattggt gccttcggga acttacatac 1020 aggtgctgca tggctgtcgt cagctcgtgt cgtgagatgt tgggttaagt cccgcaacga 1080 gcgcaaccct tttccttatt tgccagcggg ttaagccggg aactttaagg atactgccag 1140 tgacaaactg gaggaaggcg gggacgacgt caagtcatca tggcccttac gaccagggct 1200 acacacgtgc tacaatggtc ggtacaaagg gttgctacct agcgatagga tgctaatctc 1260 aaaaagccga tcgtagtccg gattggagtc tgcaactcga ctccatgaag tcggaatcgc 1320 tagtaatcgc ggatcagaat gccgcggtga atacgttccc gggccttgta cacaccgccc 1380 gtcacaccat gggaatttgt tgcaccagaa gtaggtagtc taaccgcaag gaggacgctt 1440 accacggtgt ggccgatgac tggggtgaag tc 1472 <210> 5 <211> 1485 <212> DNA <213> Bacillus vietnamensis HKMC-5 <220> <221> rRNA <222> (1)..(1485) <223> 16S rRNA <400> 5 ggctcaggac gaacgctggc ggcgtgccta atacatgcaa gtcgagcgga ttgatgggag 60 cttgctccct gatatcagcg gcggacgggt gagtaacacg tgggtaacct gcctgtaaga 120 ctgggataac tccgggaaac cggggctaat accggataac tcatttcctc gcatgaggaa 180 atgttgaaag atggcttcgg ctatcactta cagatggacc cgcggcgcat tagctagttg 240 gtgaggtaac ggctcaccaa ggcaacgatg cgtagccgac ctgagagggt gatcggccac 300 actgggactg agacacggcc cagactccta cgggaggcag cagtagggaa tcttccgcaa 360 tggacgaaag tctgacggag caacgccgcg tgagtgatga aggttttcgg atcgtaaagc 420 tctgttgtta gggaagaaca agtgccgttc gaatagggcg gcacattgac ggtacctaac 480 cagaaagcca cggctaacta cgtgccagca gccgcggtaa tacgtaggtg gcaagcgttg 540 tccggaatta ttgggcgtaa agcgcgcgca ggtggttcct taagtctgat gtgaaagccc 600 acggctcaac cgtggagggt cattggaaac tggggaactt gagtgcagaa gaggaaagtg 660 gaattccaag tgtagcggtg aaatgcgtag atatttggag gaacaccagt ggcgaaggcg 720 actttctggt ctgtaactga cactgaggcg cgaaagcgtg gggagcaaac aggattagat 780 accctggtag tccacgccgt aaacgatgag tgctaagtgt tagggggttt ccgcccctta 840 gtgctgcagc taacgcatta agcactccgc ctggggagta cggtcgcaag actgaaactc 900 aaaggaattg acgggggccc gcacaagcgg tggagcatgt ggtttaattc gaagcaacgc 960 gaagaacctt accaggtctt gacatcctct gacaacccta gagatagggc tttccccttc 1020 gggggacaga gtgacaggtg gtgcatggtt gtcgtcagct cgtgtcgtga gatgttgggt 1080 taagtcccgc aacgagcgca acccttgatc ttagttgcca gcattcagtt gggcactcta 1140 agatgactgc cggtgacaaa ccggaggaag gtggggatga cgtcaaatca tcatgcccct 1200 tatgacctgg gctacacacg tgctacaatg gacggtacaa agggcagcga gaccgcgagg 1260 tttagccaat cccataaaac cgttctcagt tcggattgta ggctgcaact cgcctacatg 1320 aagctggaat cgctagtaat cgcggatcag catgccgcgg tgaatacgtt cccgggcctt 1380 gtacacaccg cccgtcacac cacgagagtt tgtaacaccc gaagtcggtg aggtaacctt 1440 ttggagccag ccgcctaagg tgggacagat gattggggtg aagtc 1485 <210> 6 <211> 1470 <212> DNA <213> Brevibacillus invocatus HKMC-6 <220> <221> rRNA <222> (1)..(1470) <223> 16S rRNA <400> 6 ggttcaggac gaacccgggg ggcgtgctta atccatgcaa gtcgagggag ttttttcaga 60 ccctagcggc ggacgggtga gtaacacgta ggcaacctgc ccataagact gggataacat 120 agggaaactt atgctaatac cggatagagt cttctcccgc atgagagaag acggaaaggt 180 ggcgcaagct accacttgtg gatgggcctg cggcgcatta gctagttggt ggggtaacgg 240 cctaccaagg cgacgatgcg tagccgacct gagagggtga ccggccacac tgggactgag 300 acacggccca gactcctacg ggaggcagca gtagggaatt ttccacaatg gacgaaagtc 360 tgatggagca acgccgcgtg aacgatgaag gccttcgggt tgtaaagttc tgttgtcagg 420 gacgaacaag taccgttcga atagggcggt accttgacgg tacctgacga gaaagccacg 480 gctaactacg tgccagcagc cgcggtaata cgtaggtggc aagcgttgtc cggatttatt 540 gggcgtaaag cgcgcgcagg cggctatgta agtctggtgt taaagcccgg ggctcaaccc 600 cggttcgcat cggaaactgt gtagcttgag tgcagaagag gaaagcggta ttccacgtgt 660 agcggtgaaa tgcgtagaga tgtggaggaa caccagtggc gaaggcggct ttttggtctg 720 taactgacgc tgaggcgcga aagcgtgggg agcaaacagg attagatacc ctggtagtcc 780 acgccgtaaa cgatgagtgc taggtgttag gggtttcaat acccttagtg ccgcagctaa 840 cgcaataagc actccgcctg gggagtacgg tcgcaagact gaaactcaaa ggaattgacg 900 ggggcccgca caagcggtgg agcatgtggt ttaattcgaa gcaacgcgaa gaaccttacc 960 aggtcttgac atcccgctga ccgctctgga gacagagctt cccttcgggg cagcggtgac 1020 aggtggtgca tggttgtcgt cagctcgtgt cgtgagatgt tgggttaagt cccgcaacga 1080 gcgcaaccct tatctttagt tgccagcatt cagttgggca ctctagagag actgccgtcg 1140 acaagacgga ggaaggcggg gatgacgtca aatcatcatg ccccttatga cctgggctac 1200 acacgtgcta caatggttgg tacaacggga tgctacctcg cgagaggatg ccaatctctt 1260 aaaaccaatc tcagttcgga ttgtaggctg caactcgcct acatgaagtc ggaatcgcta 1320 gtaatcgcgg atcagcatgc cgcggtgaat acgttcccgg gccttgtaca caccgcccgt 1380 cacaccacgg gagtttgcaa cacccgaagt cggtgaggta accgcaagga gccagccgcc 1440 gaaggtgggg tagatgactg gggtgaagtc 1470 <210> 7 <211> 1442 <212> DNA <213> Deinococcus ficus HKMC-7 <220> <221> rRNA <222> (1)..(1442) <223> 16S rRNA <400> 7 ggctcagggt gaacgctggc ggcgtgctta agacatgcaa gtcgaacgca gtcttcggac 60 tgagtggcgc acgggtgagt aacacgtaac tgacctgccc caaagtcgcg gataactggc 120 cgaaaggtca gctaatacgt gatgtgatgt cagattctgt tctgccatta aaggtttact 180 gctttgggat ggggttgcgt tccatcagct agatggtgag gtaaaggctc accatggcga 240 cgacggatag ccggcctgag agggtggccg gccacagggg cactgagaca cgggtcccac 300 tcctacggga ggcagcagtt aggaatcttc cccaatgggc gcaagcctga gggagcgacg 360 ccgcgtgagg gatgacggtc ctcggattgt aaacctctga acccacgacg aaaggccccg 420 tatggggaga tgacggtagt ggggtaatag caccggctaa ctccgtgcca gcagccgcgg 480 taatacggag ggtgcaagcg ttacccggaa tcactgggcg taaagggcgt gtaggcggaa 540 atctaagtct ggttttaaag accgaagctc aacttcggaa gtggactgga tactggattt 600 ctagacctct ggagaggaaa ccggaattcc tggtgtagcg gtggaatgcg tagataccag 660 gaggaacacc gatggcgaag gcaggtttct ggacagaagg tgacgctgag gcgcgaaagt 720 gtggggagcg aaccggatta gatacccggg tagtccacac cctaaacgat gtacgttggc 780 ctatggcagg atgctgtcat gggcgaagct aacgcgataa acgtaccgcc tgggaagtac 840 ggccgcaagg ttgaaactca aaggaattga cgggggcccg cacaagcggt ggagcatgtg 900 gtttaattcg aagcaacgcg aagaacctta ccaggtcttg acatcccaag aacctcccag 960 agatgggagg gtgcccctcg gggaacttgg agacaggtgc tgcatggctg tcgtcagctc 1020 gtgtcgtgag atgttgggtt aagtcccgca acgagcgcaa cccttgcctt tagttgccag 1080 cacttcgggt gggcactcta gagggactgc cggtgaaagc cggaggaagg cggggatgac 1140 gtctagtcag catggtcctt acgacctggg cgacacacgt gctacaatgg atggtacaac 1200 gcgcagccag cccgcgaggg tgagcgaatc gctgaaagcc atccccagtt cagatcggag 1260 tctgcaactc gactccgtga agttggaatc gctagtaatc gcgggtcagc ataccgcggt 1320 gaatacgttc ccgggccttg tacacaccgc ccgtcacacc atgggagtac gttgcagttg 1380 aaaccgccgg gagccgcaag gcaggcgtct agactgtggc gcatgactgg ggtgaagtcg 1440 ta 1442 <210> 8 <211> 1455 <212> DNA <213> Leifsonia soli HKMC-8 <220> <221> rRNA <222> (1)..(1455) <223> 16S rRNA <400> 8 tggctcagga cgaacgctgg cggcgtgctt aacacatgca agtcgaacga tgaacctgga 60 gcttgctccg ggggattagt ggcgaacggg tgagtaacac gtgagtaacc tgcccttgac 120 tctgggataa cctccggaaa cggaagctaa taccggatat gacgtacgga ggcatctcct 180 gtacgtggaa agaatttcgg tcaaggatgg actcgcggcc tatcaggtag ttggtgaggt 240 aacggcccac caagcctacg acgggtagcc ggcctgagag ggtgaccggc cacactggga 300 ctgagacacg gcccagactc ctacgggagg cagcagtggg gaatattgca caatgggcgc 360 aagcctgatg cagcaacgcc gcgtgaggga tgacggcctt cgggttgtaa acctctttta 420 gtagggaaga agcgaaagtg acggtacctg cagaaaaagc accggctaac tacgtgccag 480 cagccgcggt aatacgtagg gtgcgagcgt tgtccggaat tattgggcgt aaagagctcg 540 taggcggtct gtcgcgtctg ctgtgaaaac ccgaggctca acctcgggcc tgcagtgggt 600 acgggcagac tagagtgcgg taggggagaa tggaattcct ggtgtagcgg tggaatgcgc 660 agatatcagg aggaacaccg atggcgaagg cagttctctg ggccgtaact gacgctgagg 720 agcgaaagcg tggggagcga acaggattag ataccctggt agtccacgcc gtaaacgttg 780 ggcgctagat gtggggacca ttccacggtt tccgtgtcgc agctaacgca ttaagcgccc 840 cgcctgggga gtacggccgc aaggctaaaa ctcaaaggaa ttgacggggg cccgcacaag 900 cggcggagca tgcggattaa ttcgatgcaa cgcgaagaac cttaccaagg cttgacatat 960 acgagaacgg gccagaaatg gtcaactctt tggacactcg taaacaggtg gtgcatggtt 1020 gtcgtcagct cgtgtcgtga gatgttgggt taagtcccgc aacgagcgca accctcgttc 1080 tatgttgcca gcgcgtaatg gcgggaactc ataggagact gccggggtca actcggagga 1140 aggtggggat gacgtcaaat catcatgccc cttatgtctt gggcttcacg catgctacaa 1200 tggccggtac aaagggctgc aataccgtaa ggtggagcga atcccaaaaa gccggtctca 1260 gttcggattg aggtctgcaa ctcgacctca tgaagtcgga gtcgctagta atcgcagatc 1320 agcaacgctg cggagaatac ctacccgggc cttgtacaca ccgcccgtca agtcatgaaa 1380 gtcggtaaca cccgaagcca ttggcccaac ccttgtggag ggagctgtcc aaggtgggat 1440 cggtgattac cagtt 1455 <210> 9 <211> 1471 <212> DNA <213> Pseudomonas nitroreducens HKMC-9 <220> <221> rRNA <222> (1)..(1471) <223> 16S rRNA <400> 9 ggctcagatt gaacgctggc ggcaggccta acacatgcaa gtcgagcgga tgagtggagc 60 ttgctccatg attcagcggc ggacgggtga gtaatgccta ggaatctgcc tggtagtggg 120 ggacaacgtt tcgaaaggaa cgctaatacc gcatacgtcc tacgggagaa agcaggggac 180 cttcgggcct tgcgctatca gatgagccta ggtcggatta gctagttggt ggggtaaagg 240 cctaccaagg cgacgatccg taactggtct gagaggatga tcagtcacac tggaactgag 300 acacggtcca gactcctacg ggaggcagca gtggggaata ttggacaatg ggcgaaagcc 360 tgatccagcc atgccgcgtg tgtgaagaag gtcttcggat tgtaaagcac tttaagttgg 420 gaggaagggc agtaagttaa taccttgctg ttttgacgtt accaacagaa taagcaccgg 480 ctaacttcgt gccagcagcc gcggtaatac gaagggtgca agcgttaatc ggaattactg 540 ggcgtaaagc gcgcgtaggt ggtttggtaa gatggatgtg aaatccccgg gctcaacctg 600 ggaactgcat ccataactgc ctgactagag tacggtagag ggtggtggaa tttcctgtgt 660 agcggtgaaa tgcgtagata taggaaggaa caccagtggc gaaggcgacc acctggactg 720 atactgacac tgaggtgcga aagcgtgggg agcaaacagg attagatacc ctggtagtcc 780 acgccgtaaa cgatgtcgac tagccgttgg gatccttgag atcttagtgg cgcagctaac 840 gcgataagtc gaccgcctgg ggagtacggc cgcaaggtta aaactcaaat gaattgacgg 900 gggcccgcac aagcggtgga gcatgtggtt taattcgaag caacgcgaag aaccttacct 960 ggccttgaca tgtccggaac cttgcagaga tgcgagggtg ccttcgggaa tcggaacaca 1020 ggtgctgcat ggctgtcgtc agctcgtgtc gtgagatgtt gggttaagtc ccgtaacgag 1080 cgcaaccctt gtccttagtt accagcacgt tatggtgggc actctaagga gactgccggt 1140 gacaaaccgg aggaaggtgg ggatgacgtc aagtcatcat ggcccttacg gccagggcta 1200 cacacgtgct acaatggtcg gtacagaggg ttgccaagcc gcgaggtgga gctaatccca 1260 taaaaccgat cgtagtccgg atcgcagtct gcaactcgac tgcgtgaagt cggaatcgct 1320 agtaatcgtg aatcagaatg tcacggtgaa tacgttcccg ggccttgtac acaccgcccg 1380 tcacaccatg ggagtgggtt gctccagaag tagctagtct aaccgcaagg gggacggtta 1440 ccacggagtg attcatgact ggggtgaagt c 1471 <210> 10 <211> 1421 <212> DNA <213> Sphingomonas aquatilis HKMC-10 <220> <221> rRNA <222> (1)..(1421) <223> 16S rRNA <400> 10 ggctcagaac gaacgctggc ggcatgccta acacatgcaa gtcgaacgag atcttcgggt 60 ctagtggcgc acgggtgcgt aacgcgtggg aatctgccct ttggttcgga ataacagttg 120 gaaacgactg ctaataccgg atgatgacga aagtccaaag atttatcgcc agaggatgag 180 cccgcgtagg attagctagt tggtgtggta aaggcgcacc aaggcgacga tccttagctg 240 gtctgagagg atgatcagcc acactgggac tgagacacgg cccagactcc tacgggaggc 300 agcagtgggg aatattggac aatgggcgaa agcctgatcc agcaatgccg cgtgagtgat 360 gaaggcctta gggttgtaaa gctcttttac ccgggatgat aatgacagta ccgggagaat 420 aagctccggc taactccgtg ccagcagccg cggtaatacg gagggagcta gcgttgttcg 480 gaattactgg gcgtaaagcg cacgtaggcg gctttgtaag ttagaggtga aagcctggag 540 ctcaactcca gaactgcctt taagactgca tcgcttgaat ccaggagagg tgagtggaat 600 tccgagtgta gaggtgaaat tcgtagatat tcggaagaac accagtggcg aaggcggctc 660 actggactgg tattgacgct gaggtgcgaa agcgtgggga gcaaacagga ttagataccc 720 tggtagtcca cgccgtaaac gatgataact agctgtccgg ggacttggtc tttgggtggc 780 gcagctaacg cattaagtta tccgcctggg gagtacggcc gcaaggttaa aactcaaatg 840 aattgacggg ggcctgcaca agcggtggag catgtggttt aattcgaagc aacgcgcaga 900 accttaccag cgtttgacat gtccggacga tttccagaga tggatctctt cccttcgggg 960 actggaacac aggtgctgca tggctgtcgt cagctcgtgt cgtgagatgt tgggttaagt 1020 cccgcaacga gcgcaaccct cgcctttagt taccatcatt tagttgggga ctctaaagga 1080 accgccggtg ataagccgga ggaaggtggg gatgacgtca agtcctcatg gcccttacgc 1140 gctgggctac acacgtgcta caatggcggt gacagtgggc agcaaactcg cgagagtgcg 1200 ctaatctcca aaagccgtct cagttcggat tgttctctgc aactcgagag catgaaggcg 1260 gaatcgctag taatcgcgga tcagcatgcc gcggtgaata cgttcccagg ccttgtacac 1320 accgcccgtc acaccatggg agttgggttc acccgaaggc gttgcgctaa ctcgcaagag 1380 aggcaggcga ccacggtggg cttagcgact ggggtgaagt c 1421 <210> 11 <211> 1419 <212> DNA <213> Methylobacterium komagatae HKMC-11 <220> <221> rRNA <222> (1)..(1419) <223> 16S rRNA <400> 11 ggctcagagc gaacgctggc ggcaggctta acacatgcaa gtcgagcggg cctttcgggg 60 tcagcggcgg acgggtgagt aacgcgtggg aacgtgcctt ctggttcgga ataactcagg 120 gaaacttgag ctaataccgg atacgccctt ttggggaaag gtttactgcc ggaagatcgg 180 cccgcgtctg attagctagt tggtggggta acggcctacc aaggcgacga tcagtagctg 240 gtctgagagg atgatcagcc acactgggac tgagacacgg cccagactcc tacgggaggc 300 agcagtgggg aatattggac aatgggcgca agcctgatcc agccatgccg cgtgagtgat 360 gaaggcctta gggttgtaaa gctcttttat ccgggacgat aatgacggta ccggaggaat 420 aagccccggc taacttcgtg ccagcagccg cggtaatacg aagggggcta gcgttgctcg 480 gaatcactgg gcgtaaaggg cgcgtaggcg gtcttttaag tcgggggtga aagcctgtgg 540 ctcaaccaca gaattgcctt cgatactggg agacttgagt tcggaagagg ttggtggaac 600 tgcgagtgta gaggtgaaat tcgtagatat tcgcaagaac accggtggcg aaggcggcca 660 actggtccga cactgacgct gaggcgcgaa agcgtgggga gcaaacagga ttagataccc 720 tggtagtcca cgccgtaaac gatgaatgcc agccgttggg gggcttgcct ctcagtggcg 780 cagctaacgc tttgagcatt ccgcctgggg agtacggtcg caagattaaa actcaaagga 840 attgacgggg gcccgcacaa gcggtggagc atgtggttta attcgaagca acgcgcagaa 900 ccttaccatc ctttgacatg gcgtgttacc cagagagatt tggggtccac ttcggtggcg 960 cgcacacagg tgctgcatgg ctgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc 1020 gcaacgagcg caacccacgt cctcagttgc catcattgag ttgggcactc tggggagact 1080 gccggtgata agccgcgagg aaggtgtgga tgacgtcaag tcctcatggc ccttacggga 1140 tgggctacac acgtgctaca atggcggtga cagtgggaag cgaacccgcg aggggaagca 1200 aatctccaaa agccgtctca gttcggattg cactctgcaa ctcgagtgca tgaaggcgga 1260 atcgctagta atcgtggatc agcatgccac ggtgaatacg ttcccgggcc ttgtacacac 1320 cgcccgtcac accatgggag ttggtcttac ccgacggcgc tgcgccgacc cgcgagggga 1380 gcaggcgacc acggtagggt cagcgactgg ggtgaagtc 1419

Claims (13)

Microorganisms used for coating with odorless biofilms that do not cause odor in the air conditioner are methyl bromide bacteria AQUATICUM HKMC-1 ( Methylobacterium aquaticum HKMC-1) (KCCM11325P).
The method according to claim 1, wherein the methylobacterium Aqua tikum HKMC-1 (Methylobacterium aquaticum HKMC- 1) is a methyl, it characterized in that it comprises a gene of SEQ ID NO: 1 tumefaciens AQUATICUM HKMC-1 ( Methylobacterium aquaticum HKMC-1) (KCCM11325P).
Microbial methyl bromide AQUATICUM HKMC-1 ( Methylobacterium aquaticum HKMC-1) (KCCM11325P) An odorless EVA core that does not cause odors in an air conditioner coated with a biofilm formed by breeding a strain under aerobic conditions.
4. The method according to claim 3, wherein the microorganism methylobacterium Aqua tikum the EVA core coated with a biofilm formed by breed (Methylobacterium aquaticum) is a methyl in addition to the above microorganisms tumefaciens Beuraki Atum (Methylobacterium brachiatum), a bacterium methyl Solarium flash Tani (Methylobacterium platani), Acinetobacter Jones you (Acinetobacter johnsonii), Bacillus Corbett namen sheath (Bacillus vietmanensis ), Brevibacillus Invoke car Bluetooth (Brevibacillus invocatus), Day Noko kusu blood Syracuse (Deinococcus ficus ), rape sonia Soli (Leifsonia soli), Pseudomonas Knight Lori Dew sense (Pseudomonas nitroreducens), Sphingomonas Aqua Antilles (sphingomonas aquatilis ) and methyl &lt; RTI ID = 0.0 &gt; The coma state (Methylobacterium The present invention relates to an odorless EVA core which does not cause odor in an air conditioner, which further comprises one or more microorganisms selected from the group consisting of komagatae and komagatae .
[4] The exhaust gas purifying apparatus according to claim 3, wherein the eva core is made of aluminum, an aluminum alloy, copper, or a copper alloy.
(1) On the surface to which the medium was applied, microbial methyl bromide Aqua tikum HKMC-1 (Methylobacterium aquaticum HKMC- 1) (KCCM11325P) Attaching the strain; and
(2) The bacterium as methyl Solarium AQUATICUM HKMC-1 ( Methylobacterium aquaticum HKMC-1) (KCCM11325P) in an aerobic condition to form a biofilm;
Wherein the method comprises the steps of:
The method of claim 6 wherein the methyl in the above step (1) tumefaciens Aqua tikum (Methylobacterium aquaticum) in addition to the bacterium methyl Solarium beuraki Atum (Methylobacterium brachiatum ), methyl &lt; RTI ID = 0.0 &gt; Platani (Methylobacterium platani), Acinetobacter Jones you (Acinetobacter johnsonii), Bacillus Corbett namen sheath (Bacillus vietmanensis ), Brevibacillus Invoke car Bluetooth (Brevibacillus invocatus), Noko Day Nukus Nukus blood (Deinococcus ficus), Leif Sonia Soleil (Leifsonia soli), Pseudomonas Knight Lori Dew sense (Pseudomonas nitroreducens), Sphingomonas Aqua Antilles (sphingomonas aquatilis ) and methyl &lt; RTI ID = 0.0 &gt; A coma state (Methylobacterium komagatae) does not exert a smell in the air conditioning device, comprising a step of coating the one or two or more microorganisms selected from the group consisting of additional biofilm in odorless EVA core manufacturing method.
7. The method of claim 6, wherein the surface of the core is made of aluminum, an aluminum alloy, copper, or a copper alloy, and does not cause odor in the air conditioner.
The microbial methyl bromide bacteria Aqua tikum HKMC-1 (Methylobacterium aquaticum HKMC- 1) (KCCM11325P) strain by coating by propagation in an aerobic condition within the air conditioning system that does not exert a smell odorless biofilm.
10. The method of claim 9, wherein the biofilm is odorless to the methyl tumefaciens aqua tikum (Methylobacterium aquaticum) in addition to the bacterium methyl Solarium beuraki Atum (Methylobacterium brachiatum ), methyl &lt; RTI ID = 0.0 &gt; Platani (Methylobacterium platani), Acinetobacter Jones you (Acinetobacter johnsonii), Bacillus Corbett namen sheath (Bacillus vietmanensis), Breda ratio Bacillus car inboard tooth (Brevibacillus invocatus), Day Noko kusu blood Syracuse (Deinococcus ficus), Leif Sonia Soleil (Leifsonia soli), Pseudomonas Knight Lori Dew sense ( Pseudomonas nitroreducens), Sphingomonas Aqua Antilles (sphingomonas Solarium coma bacterium to aquatilis) methyl and the state (Methylobacterium The present invention relates to an odorless biofilm that does not cause odor in an air conditioner, which further comprises one or more microorganisms selected from the group consisting of komagatae and komagatae .
Microbial methyl bromide AQUATICUM HKMC-1 ( Methylobacterium aquaticum HKMC-1) (KCCM11325P), which does not cause the odor caused by the biofilm formed by propagating the strain in aerobic conditions.
12. The method of claim 11, wherein the cooling device is in the bacterium methyl Solarium Aqua tikum (Methylobacterium aquaticum) in addition to the bacterium methyl Solarium beuraki Atum (Methylobacterium brachiatum ), methyl &lt; RTI ID = 0.0 &gt; Platani (Methylobacterium platani), Acinetobacter Jones you (Acinetobacter johnsonii), Bacillus Corbett namen sheath (Bacillus vietmanensis), Breda ratio Bacillus car inboard tooth (Brevibacillus invocatus), Day Noko kusu blood Syracuse (Deinococcus ficus), Leif Sonia Soleil (Leifsonia soli), Pseudomonas Knight Lori Dew sense ( Pseudomonas nitroreducens), Sphingomonas Aqua Antilles (sphingomonas Solarium coma bacterium to aquatilis) methyl and the state (Methylobacterium wherein the microorganism is further coated with one or more microorganisms selected from the group consisting of chlorophyll and komagatae .
12. The air conditioning system of claim 11, wherein the air conditioning system includes an eva core.

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