KR101508164B1 - Composition for controlling plant viruses comprising shikimic acid as effective ingredient - Google Patents

Abstract

The present invention relates to a plant virus control method comprising a step of treating a plant with a composition for controlling plant viruses containing shikimic acid as an active ingredient and a composition containing shikimic acid as an effective ingredient.

Description

[0001] The present invention relates to a composition for controlling plant viruses containing shikimic acid as an active ingredient,

The present invention relates to a composition for controlling a plant virus containing shikimic acid as an active ingredient, and more particularly to a composition for controlling a plant virus containing shikimic acid as an active ingredient and a process for treating the plant with the composition To a plant virus control method.

The annual production of red pepper is about 1 trillion won for non-cultivated red pepper and about 500 billion won for red pepper cultivated by plant. Viral infections gradually increase from sowing to late maturity through various transmission routes. At the end of the growth period, most peppers show marked viral symptoms in the case of green pepper, and the reduction in production due to the virus is estimated to be about 30% on average. In the case of the red pepper grown in the facility, the reduction in production due to viral diseases is estimated to be about 10%. In the case of virus infection, unlike other diseases of pepper, there is no direct control agent, so if the damage occurs, serious damage is seen and it is not possible to establish clear control measures yet.

There are four types of viruses in domestic pepper: Pepper mild mottle virus (PMMoV), Cucumber mosaic virus (CMV), Pepper mottle virus (PepMOV) (Broad bean wait virus, BBWV) accounts for most of the viral diseases.

Peppermile mild mottle viruses are widely grown in the country and are a major source of exported crops such as sweet pepper and sweet pepper, which have high economic value. The pepper mild mottle virus is easily transmitted by contaminated seeds or mechanical contact, causing economic damage. In many cases, yellow stain patterns appear first, and the overall growth is poor and necrotic tissue appears in the twigs. In leaf, mosaic is accompanied by wrinkle and wrinkle or curled forward.

Korean Patent No. 10-0823085 discloses a composition for controlling plant viruses derived from natural products, and Korean Patent No. 10-0738647 discloses a plant virus control agent containing an extract of Aspergillus as an active ingredient.

The present invention has been made in view of the above-described needs. The present inventors have completed the present invention by confirming that a natural compound extract having antiviral activity is screened and that a specific compound in the extract inhibits the infection of peppermil mite mottle virus .

In order to solve the above problems, the present invention provides a composition for controlling a plant virus containing shikimic acid as an active ingredient.

The present invention also provides a plant virus control method comprising treating a plant with a composition containing shikimic acid as an effective ingredient.

According to the present invention, the composition for controlling plant viruses of the present invention is effective for inhibiting the infection of pepper mild mottle virus, and thus it is considered to be useful for improving the productivity of pepper. Further, the composition of the present invention is environment-friendly as a material derived from a natural product and has high safety because it is free from toxicity.

In order to achieve the object of the present invention, the present invention provides a composition for controlling plant viruses containing shikimic acid as an active ingredient. The plant viruses may be selected from the group consisting of peppermilm mottle virus (PMMoV), tobacco mosaic virus (TMV), cucumber mosaic virus (CMV), zucchini macular mucosa virus (ZYMV), watermelon mosaic virus (WMV), amber mosaic virus (PVY), TuMV, MNSV, CGMMV, ZGMMV, PLRV, LMoV, (ORSV), Cymbidium Mosaic Virus (CyMV), PemMOV, Peppermint Virus (BBWV), Tomato Yunpus Virus (TomRSV) or Tomato Sulfur Virus Gangrene virus (TRSV), but are not limited thereto. Preferably, the plant virus is pepper mild mottle virus (PMMoV).

The shikimic acid, which is an effective ingredient of the plant virus control composition, can be produced by natural products or by organic chemical synthesis, but is not limited thereto. Preferably, the shikimic acid may be prepared from a natural product sample by ethanol or methanol extraction. The ethanol or methanol extraction process is well known in the art. For example, after a natural sample is dried and ground to make powder, each powder sample is placed in a flask, methanol is added, and the mixture can be extracted by shaking at 100 rpm for 24 hours. The extracted material can be concentrated under reduced pressure and lyophilized.

The composition for controlling plant viral diseases according to the present invention can be prepared, for example, in the form of directly sprayable solutions, powders and suspensions or in the form of highly concentrated aqueous, oily or other suspensions, dispersions, emulsions, oily dispersions, pastes, dusts, But is not limited thereto. In addition, the plant virus control composition may be used by spraying, spraying, spraying, scattering or swelling. The mode of use depends on the intended purpose, and in all cases the distribution of the composition according to the invention should be as fine and uniform as possible.

The composition for controlling a plant virus of the present invention can be formulated into various forms. The preparation may be prepared, for example, by adding a solvent and / or a carrier. Often, inert additives and surface-active materials, such as emulsifiers or dispersants, are mixed into the formulation. Suitable surface-active materials include, but are not limited to, aromatic sulfonic acids (e.g., lignosulfonic acid, phenol-sulfonic acid, naphthalene- and dibutylnaphthalenesulfonic acid), fatty acids, alkyl- and alkylarylsulfonates, alkyl lauryl ethers, A salt of a fatty alcohol glycol ether, a sulfonate naphthalene and derivatives thereof, a condensate of formaldehyde, a condensate of naphthalene or naphthalene sulfonic acid, a phenol and a formaldehyde Condensates, polyoxyethylene octylphenol ethers, ethoxylated isooctyl-, octyl- or nonylphenol, alkylphenyl or tributylphenyl polyglycol ethers, alkylaryl polyether alcohols, isotridecyl alcohol, fatty alcohol / ethylene oxide condensates , Ethoxylated castor oil, polyoxyethylene alkyl ether or polyoxypropylene, lauryl alcohol Polyglycol ether acetate, sorbitol esters, lignin-sulfite waste liquid, or may be a cellulose, are not limited. Suitable solid carrier materials are in principle all porous and comprise an agriculturally acceptable carrier such as mineral earths such as silica, silica gel, silicate, talc, kaolin, limestone, limestone, chalk, Fertilizers such as ammonium sulfate, ammonium phosphate, ammonium nitrate, urea, vegetable products such as cereal flour, bark powder, wood meal, And nut shell powder), or cellulose powder. The solid carrier may be used alone or in combination of two or more.

The composition for controlling a plant virus of the present invention can be used for, but not limited to, a tube-receiving, a leaf-surface spraying, a seed sterilization, or an agricultural equipment disinfection.

The composition for controlling a plant virus of the present invention can be used as an effective ingredient by mixing shikimic acid alone or in combination with two or more different antiviral substances.

In addition, the composition for controlling a plant virus of the present invention may be formulated into a pesticide formulation, further comprising an agricultural chemical having a controlling effect against plant infectious diseases.

In addition, the composition for controlling plant viral diseases can be used in combination with a dispersing agent, a penetrating agent, or a surfactant to improve crop absorption and effect.

The present invention also provides a plant virus control method comprising treating a plant with a composition containing shikimic acid as an effective ingredient. The plant viruses may be selected from the group consisting of peppermilm mottle virus (PMMoV), tobacco mosaic virus (TMV), cucumber mosaic virus (CMV), zucchini macular mucosa virus (ZYMV), watermelon mosaic virus (WMV), amber mosaic virus (PVY), TuMV, MNSV, CGMMV, ZGMMV, PLRV, LMoV, (ORSV), Cymbidium Mosaic Virus (CyMV), PemMOV, Peppermint Virus (BBWV), Tomato Yunpus Virus (TomRSV) or Tomato Sulfur Virus Gangrene virus (TRSV), but are not limited thereto. Preferably, the plant virus is pepper mild mottle virus (PMMoV).

The composition for controlling plant viruses can be used for disinfection of agricultural equipments during the agricultural work such as foliage treatment, soil treatment, immersion treatment, branch cutting, etc. to prevent many plant virus infections. In addition, the composition may be pretreated to plants before the plant is infected with the virus.

Hereinafter, the present invention will be described in detail by way of examples. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

Ⅰ. Materials and methods

One. Virus source  And viral bioactive screening plants

The virus was exposed to tobacco virus, pepper mild mottle virus (PMMoV), tobacco ( Nicotiana tabacum cv. (W / V) 0.01 M phosphate buffer (pH 7.0) was added to the tobacco leaf, and the mixture was sieved with two layers of gauze. The juice was used as an inoculum. The inoculum was diluted and used constantly to form 200-300 lesions on Xanthi nc. Zanthi nc, a host disease causing resistance to PMMoV, was used for the inoculation, and Xanthi nc, which indicates systemic symptoms such as mosaic, was used as the systemic infection group. Were used for 5 to 6 leaf stage plants grown for 4 weeks.

2. Bioactivity Assay

The PMMoV infection inhibitory activity assay was performed using the half leaves method using Zanthi nc, a resistant plant against the virus. Shikimic acid is sprayed on 2 ~ 4 half leaves per plant and leaves are treated with distilled water for 1 ~ 24 hours. The infection inhibition effect is estimated by counting the number of lesions of the shikiman acid-treated bark and the untreated (distilled water) bark after 2 to 5 days of inoculation.

(%) = ((Number of diseased lesions in the control group - number of lesions in the treatment group) / number of lesions in the control group) × 100

3. Shkiman  Local infection local lesion infection ) Antiviral (Infection Control) Effect in Host Plants

end. Effect of mixing treatment

In order to investigate whether the sheikim acid acts on virus inactivation and exhibits antiviral effect, the virus is mixed with 1% (W / V) of the shear acid in the diluted virus, and this solution is inoculated on the Xanthi nc leaf with the half- The number of local lesions formed after 5 days was compared with the control.

I. Effect on pretreatment

In order to investigate the effect of shikimic acid on permeability, adhesion, or stability to leaf tissue, shikiman acid was applied to the half leaves of Zanthi nc using a brush and the remaining half lobes were treated with distilled water to give a control. One hour after the treatment, PMMoV was inoculated and the number of local lesions after 2-5 days was examined.

Example  1: local infection At the host  Infectious effect of infection

end. Effect of mixing treatment

Shymic acid was mixed with 5 mg / ml and 10 mg / ml and treated with the half - lobe method on the black plant Xanthi nc leaves, and the virus inhibitory effect was 71.2% at the concentration of 10 mg / ml.

Inhibitory effect of mixed chemotherapeutic agent of Schmicken and PMMoV on local lesion infection Treating material Black plant Processing method Survey week Treatment substance concentration (mg / ml) Inhibition rate of infection ^a) (%) Shikiman Xanthi nc Semi-circular method 3 5 52.7 Shikiman Xanthi nc Semi-circular method 3 10 71.2

a) Inhibition rate of infection: ((number of antinuclear half-leaf lesions-number of half-leaf lesions in treatment) / number of half-round lesions in non-treatment side) × 100

I. Effect on pretreatment

To evaluate the adhesion or safety of Xanthi nc, the host plant of Shimjiman PMMoV, the host plants were shaken for 2 hours, and PMMoV was inoculated on the leaf surface. As a result, in Xanthi nc treated with 5 mg / ml, sheikimic acid showed 37.5% and 81.4% at the concentration of 10 mg / ml.

Inhibitory effect of PMMoV on local lesion infection after pretreatment with shikiman Treating material Black plant Processing method Survey week Treatment substance concentration (mg / ml) Inhibition rate of infection ^a) (%) Shikiman Xanthi nc Semi-circular method 3 5 37.5 Shikiman Xanthi nc Semi-circular method 3 10 81.4

a) Inhibition rate of infection: ((number of antinuclear half-leaf lesions-number of half-leaf lesions in treatment) / number of half-round lesions in non-treatment side) × 100

Claims (4)

A composition for controlling pepper mild mottle virus (PMMoV) containing shikimic acid as an active ingredient. delete A method for controlling pepper mild mottle virus (PMMoV) comprising treating a plant with a composition containing shikimic acid as an active ingredient. delete