KR101437634B1 - Composition for hangover solution using plant extract - Google Patents

Abstract

The present invention relates to a composition for hangover decomposing hair comprising a root extract of Brassica officinalis, and more particularly to a composition for hanging roots containing an extract of any one or more selected from the group consisting of roots, The present invention relates to a composition. When beverages, medicines, foods and the like are prepared and consumed by using the composition for hangover drowning according to the present invention, the activity of ALDH is increased to achieve an excellent effect on hangover resolution, and the content of glutathione And the recovery of liver function to produce albumin. It also lowers the amount of LDL-cholesterol that is caused by alcohol, thus preventing arteriosclerosis despite drinking alcohol.

Description

[Technical Field] The present invention relates to a composition for hangover solution using a plant extract,

More particularly, the present invention relates to a composition for hangover decoy comprising roots, leaves and stem extracts of hair buds as an active ingredient.

It is a perennial plant that grows up to 120 cm in height around wetlands and wetlands, and is a plant belonging to Lythraceae, distributed throughout the world, and used as a medicinal or hermaphroditic material in Korea (Lee, 1996; Kim and Lee, 1992) .

According to the data of the National Statistical Office, from 2005 to 2009, 14 ~ 33% of Korean males aged 19 ~ 70 years old had high-risk drinking, especially 40 ~ 49 year old males had a very high drinking rate of more than 32% There is a risk of harming your health.

On the other hand, after a considerable amount of drinking, you may experience hangover pain for at least a while, which is known to be caused by the alcohol metabolite acetaldehyde. That is, the consumed alcohol is decomposed into water and acetaldehyde by an alcohol dehydrogenase (ADH), and the acetaldehyde thus decomposed is decomposed by an aldehyde dehydrogenase (hereinafter referred to as ALDH) And then decomposed again into acetic acid. Therefore, the alcoholysis enzymes such as ADH and ALDH play an important role in hangovers.

However, there have been no studies to apply hangover treatment in association with the alcohololytic enzyme of this bacterium.

It is an object of the present invention to provide a composition for relieving hangover, comprising roots, leaves and stem extracts of hairy buds as an active ingredient. It is also intended to provide a composition that increases the content of glutathione (GSH) which is effective in removing active oxygen in liver tissue. Also, it is intended to provide a composition for rapidly restoring liver function and preventing arteriosclerosis caused by alcohol. It is also intended to provide a composition which is effective in relieving hangover despite single administration.

In order to solve the above problems, one aspect of the present invention provides a composition for hangover decomposing, which comprises as an active ingredient, one or more extracts selected from the group consisting of roots, leaves and stems of hair buds. And the extract is contained in an amount of 0.0001 to 99.9999% by weight.

A beverage according to another aspect of the present invention is prepared by using a composition for a hangover decoy containing, as an active ingredient, at least one extract selected from the group consisting of roots, leaves and stems of hair buds.

The health supplement according to another aspect of the present invention is prepared by using a composition for a hangover decoy containing, as an active ingredient, any one or more extracts selected from the group consisting of roots, leaves, and stems of a hairy bud.

The composition for a hangover decoy according to the present invention relates to a composition exhibiting an excellent effect on hangover resolution. Specifically, it enhances the activity of ALDH to help relieve hangover, and maintains high albumin content in the blood even after drinking, which has the effect of rapidly restoring liver function. It also has the effect of keeping the amount of reduced glutathione, which has an antioxidative effect, high in liver tissue. In addition, despite the intake of alcohol, the amount of LDL cholesterol in the arteries is reduced, thus preventing arteriosclerosis caused by alcohol consumption. It is also effective in eliminating hangovers even when administered once.

FIG. 1 is a graph showing the results of measurement of ALDH activity in Examples 2-1 to 2-3, the control group, and Comparative Example 1 and Comparative Example 2 of the present invention.
2 is a graph showing the results of measuring the amounts of reduced glutathione in Examples 2-1 to 2-3 of the present invention, a control group, and Comparative Example 1 and Comparative Example 2. Fig.
3 is a graph showing the results of measurement of the amounts of albumin of Examples 2-1 to 2-3, the control group, the comparative example 1 and the comparative example 2 of the present invention.
4 is a graph showing the results of measuring the activity of ADH after single administration of the extract of Example 4 and Comparative Example 3 and the rat of Comparative Example 4. Fig.
FIG. 5 is a graph showing the results of measuring the activity of ALDH after single administration of the extract of Example 4 and Comparative Example 3 and the rat of Comparative Example 4. FIG.
FIG. 6 is a graph showing the results of measuring the amount of total glutathione after the single administration of the extract of Example 4 and Comparative Example 3 and the rat of Comparative Example 4; FIG.
FIG. 7 is a graph showing the results of measuring the values of oxidized glutathione / total glutathione after the single administration of the extract of Example 4 and Comparative Example 3 and the rat of Comparative Example 4; FIG.
FIG. 8 is a graph showing the results of measuring the amount of albumin after the single administration of the extract of Example 4 and Comparative Example 3 and the rat of Comparative Example 4;
FIG. 9 is a graph showing the results of measuring the amounts of HDL-cholesterol after the single administration of the extract of Example 4 and Comparative Example 3 and the rat of Comparative Example 4. FIG.

Accordingly, the present inventors have made extensive efforts to overcome the problems of the prior arts, and as a result, it is possible to provide a composition containing roots, leaves and stem extracts of hairy bouche flowers having excellent efficacy for relieving hangover according to the present invention as active ingredients And completed the present invention.

Specifically, the composition for a hangover remedy of the present invention may contain, as an active ingredient, any one or more extracts selected from the group consisting of roots, leaves and stems of a hairy flower.

Generally, in the hangover resolution, the alcohol is decomposed into water and acetaldehyde by ADH, and the acetaldehyde thus decomposed is again decomposed by ALDH, and it is known that hangover is solved. Therefore, in the end, ALDH has a great influence on the hangover resolution, and the root, leaf and stem extracts of the hair buds according to the present invention have the effect of increasing the activity of the ALDH and thus effective in eliminating the hangover. In addition, the roots, leaves and stem extracts of hairy buds according to the present invention have an effect of increasing the content of glutathione, which is an antioxidant, in the liver tissue and the content of albumin in the serum in spite of alcohol consumption, It is confirmed. Because albumin is produced in the liver, an increase in the albumin content in the blood indicates that the damaged liver function has been recovered by drinking, which also means that the hangover has been resolved.

In addition, the roots, leaves and stem extracts of the hairy buds of the hangover decay composition may preferably be contained in an amount of 0.0001 to 99.9999% by weight. Water as an additive other than the above extract may preferably contain 0.0001 to 99.9999% by weight. The liquid fructose as an additive other than the above extract is preferably 0 to 0.5% by weight, the oligosaccharide is preferably 0 to 2% by weight, the sugar is preferably 0 to 2% by weight, the salt is preferably 0 to 0.5% % ≪ / RTI >

When the hanging composition is prepared according to the above composition range, it is possible to maximize the content of ALDH activity, glutathione and albumin, and exhibits a rich aesthetic effect in the production thereof. In addition, the roots, leaves and stem extract Thereby preventing damage.

The roots, leaves and stem extracts of the hairy buds may be extracted from a mixture of water and alcohol, more preferably extracted with ethanol (alcohol) can do.

As a further feature of the present invention, a beverage using a hangover composition containing an extract of any one or more selected from the group consisting of roots, leaves and stems of the hairy flower of the present invention increases the activity of ALDH, . It also has the effect of keeping the content of glutathione, an antioxidant, and albumin produced in the liver at a high level. The beverage includes all of the beverages produced by a conventional method, but may preferably include a drink, tea, juice, and the like.

As a further feature of the present invention, the health supplement using the composition for hangover drowning, which comprises, as an active ingredient, at least one extract selected from the group consisting of roots, leaves and stems of the hairy flower, increases the activity of ALDH It is effective in eliminating hangovers. It also has the effect of keeping the content of glutathione, an antioxidant, and albumin produced in the liver high. The health supplements include all the health supplements produced by conventional methods, but preferably include pills, potions, injections, and the like.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

< Example  One: Hairy  Preparation of roots, leaves and stem extracts>

Hairy buds were collected from Soi-myeon, Nogun-gun, Chungcheongbuk-do, and classified into root, leaf and stem, washed, and dried. Dried roots, leaves and stems were ground into powder. Then, each powder was extracted with 50% ethanol at 85 ° C using a reflux extracting apparatus to prepare extracts of roots, leaves and stems, respectively. The solvent contained in the extract thus prepared was removed by concentration under reduced pressure and freeze drying to prepare roots, leaves, and stem extracts of Budae. Hereinafter, the root extract of Fusarium oxyspora is referred to as Example 1-1, the leaf extract as Example 1-2, and the stem extract as Example 1-3.

< Example  2: Hairy  Roots, leaves, and stem extracts for 10 weeks>

Rats between 170 and 200 g body weight (SPF grade / week, BioLink, negative) were purchased and adapted to the breeding environment for 1 week. Twelve of them were administered 30 ~ 30 ㎖ of ethanol 30 ~ 40 ㎖ divided into morning and afternoon for 6 days / week for 10 weeks and administered twice a day. The root extract of Example 1-1 dissolved in a mixture of corn oil and distilled water 300 mg once a day for 6 days / week for 10 weeks. And twelve of the leaf extracts according to Example 1-2, which were dissolved in a mixture of corn oil and distilled water while being administered 30 to 30 ml of ethanol 30-40 ml per day for 6 days / 300 mg once a day for 6 days / week for 10 weeks. Lastly, twelve animals were administered with 30 to 40 ml of 30% ethanol for 6 days / week for 10 weeks, divided into morning and afternoon, twice a day, and the stem according to Example 1-3 dissolved in a mixture of corn oil and distilled water 300 mg of the extract was orally administered once / day for 6 days / week for 10 weeks. A group of rats to which the extract of Example 1-1 was administered was referred to as Example 2-1, a group of rats to which the extract of Example 1-2 was administered was referred to as Example 2-2, The group of rats to which the extract was administered is referred to as Example 2-3.

< Example  3: Hairy Buddha  Safety of Root Extract>

The genetic toxicity of the root extract of Brassica chinensis extracted from Example 1-1 was analyzed to confirm its safety. This experiment was conducted by GLP testing institute (Biotoxtech Co., Ltd.). Among them, the cells were cultured with a histidine-binding Salmonella (TA98, TA100, TA1535 and TA1537) and tryptophan-producing E. coli (PKM101) on a hairy root extract extract plate. Thus, the possibility of gene mutagenesis in the strain was observed, but all of them were confirmed by negative. In addition, root extracts of Brassica spp. Were orally administered to male rats, and bone marrow cells and somatic cells were extracted to confirm the presence or absence of abnormalities. There was no abnormality in bone marrow cells, and no abnormal chromosome was found in somatic cells. Therefore, it is considered that root extract of hairy budgie does not have a genetic adverse effect on the human body even if it is ingested.

< Example  4: Hairy Buddha  Root extract One time  Administration>

The rats were orally administered with 20% ethanol of 30% on the day before the autopsy, and after 20 hours of 30% ethanol was once administered once again on the day of the autopsy 17 hours later, and after 1 hour, 900 mg / kg of extract was orally dissolved in a mixture of corn oil and distilled water. Autopsy and blood collection were performed 1 hour after oral administration of the extract to each rat.

Normal group

Twelve rats (specific pathogen free (SPF) grade / week, Korean BioLink, negative) between 170 and 200 g body weight were purchased and adapted to the breeding environment for one week. Unlike Example 2, Only the same amount of corn oil and distilled water as in Examples 1-2 and 1-3 was orally administered to rats for 1 day / day, 6 days / week, and 10 weeks, and no alcohol was administered .

Comparative Example  One

Twelve rats (specific pathogen free (SPF) grade / week, Korean BioLink, negative) between 170 and 200 g body weight were purchased and adapted to the breeding environment for one week. Unlike Example 2, The same amount of corn oil and distilled water as in Examples 1-2 and 1-3 was administered once / day, 6 days / week, and 10 weeks, and 30-40 ml of 30% ethanol 6 days / week, 10 weeks in the morning and afternoon.

Comparative Example  2

Twelve rats (specific pathogen free (SPF) grade / week, Korean BioLink, negative) between 170 and 200 g body weight were purchased and adapted to the breeding environment for one week. In contrast to Example 2, 30% 30 or 40 ml of ethanol was divided into morning and afternoon for 6 days / week for 10 weeks. Oral administration was carried out, and 300 mg of the flower extract of Curcuma chinensis extracted in the same manner as in Example 1 was dissolved in a mixture of corn oil and distilled water 1 / day, 6 days / week, and 10 weeks.

Comparative Example  3

An extract of Hovenia dulcis (HD) sold in the market was used in the same manner as in Example 3. That is, in another rat, 20 ml of ethanol of 30% on the day before the necropsy was once administered orally, and then 20 ml of 30% ethanol was once administered once to the necropsy day after 17 hours. After 1 hour, 900 mg / kg of tree and bottle extract was dissolved in a mixture of corn oil and distilled water and orally administered. Autopsy and blood collection were performed 1 hour after oral administration of the extract to each rat.

Comparative Example  4

The rats according to Comparative Example 4 were orally administered with 20 ml of 30% ethanol on the day before the necropsy test. After 17 hours of oral administration, 20 ml of 30% ethanol was again administered once, and after 1 hour, oil) and distilled water. Autopsy and blood collection were performed 1 hour after oral administration of the extract to each rat.

Experimental results for Example 2, normal group, comparative example 1 and comparative example 2 were shown by averaging the analysis values for eight rats minus two maximum values and two minimum values.

The mean values of the different alphabetic characters in the superscripts were significant at the p <0.05 level when the one way ANOVA test and the Duncan's multiple range test were performed.

< Experimental Example  One: ALDH  Activity analysis>

In each group of rats according to Example 2-1, Example 2-2, Example 2-3, normal group, Comparative Example 1 or Comparative Example 2, after 10 weeks of oral administration period, Were all autopsied to obtain liver tissue. The obtained liver tissues were weighed, and 4 times of 0.1 M potassium phosphate buffer (pH 7.4) was added thereto, and the mixture was ground with a tissue mill in ice to obtain an enzyme solution containing the enzyme. The obtained enzyme solution was centrifuged at 600 xg for 10 minutes, and the supernatant was centrifuged at 10,000 x g for 20 minutes to obtain a mitochondrial fraction. The mitochondrial fraction thus obtained was assayed for ALDH activity. Activity assays for ALDH include Kathryn et al. (Sodium pyrophosphate, pH 8.8) containing 2 ml of 1 mM NAD, 0.2 mM of 4-methyl pyrazole and 1 mM of magnesium chloride, ) And 1 ml of 2 μM rotenone were mixed with 0.4 ml of enzyme solution and incubated in a water bath at 30 ° C for 20 minutes and 0.2 ml of 5 mM acetaldehyde was added thereto to measure the absorbance at 340 nm for 1 minute And the amount of NADH produced per minute at 340 nm was calculated by applying an extinction coefficient (6220 M -1 cm -1). Protein content was calculated by Bradford (1976) method using bovine serum albumin as a standard. The results are shown in Table 1 and FIG. 1 below. The cytosol fraction obtained by ultracentrifugation of the obtained supernatant at 105,000 × g for 1 hour was used for the analysis of alcohol dehydrogenase (ADH) activity.

Table 1 shows the range of the maximum amount and the minimum amount with respect to the average value in addition to the average value of the ALDH activity, and FIG. 1 is a graph showing only the average value. As can be seen from FIG. 1, the average value according to Example 2-1, Example 2-2, and Example 2-3 is significantly higher than that of the normal group, Comparative Examples 1 and 2. Therefore, oral intake of root, leaf or stem extracts from the hairy budgie increased the activity of ALDH, which affects hangover resolution in the liver.

< Experimental Example  2: Glutathione  Content analysis>

Each rat according to Example 2-1, Example 2-2, Example 2-3, normal group, Comparative Example 1 and Comparative Example 2 was autopsied and liver was extracted. The hepatic tissues thus obtained were sectioned and weighed, followed by 4 times of 0.1 M potassium phosphate buffer (pH 7.4), and the mixture was ground with a tissue mill in ice to obtain an enzyme solution containing the enzyme. Glutathione (GSH) content was determined by the method of Ellman (1959) and S'wiergosz-Kowalewska et al. (2006). The total glutathione content was analyzed first and then the value of oxidized glutathione The content was subtracted from the reduced glutathione. The results are shown in Table 2 and FIG. 2 below.

Reduced glutathione refers to the addition of SH (S is sulfur, H is hydrogen) to glutathione. This reduced glutathione directly plays a role in removing harmful active oxygen. Table 2 shows the range of the maximum and minimum amounts of the average value in addition to the average value of the reduced glutathione, and FIG. 2 is a graph showing only the average value. 2, the amount of reduced glutathione was greater in the case of Example 2-1, Example 2-2 and Example 2-3 than in Comparative Example 1 and Comparative Example 2, 2 was significantly increased compared to the normal group. Therefore, it can be confirmed that Examples 2-1, 2-2, and 2-3 increase the amount of reduced glutathione in spite of alcohol consumption, thereby contributing to antioxidative action.

< Experimental Example  3: Measurement of albumin content>

In each group of rats according to Example 2-1, Example 2-2, Example 2-3, normal group, Comparative Example 1 or Comparative Example 2, each mouse was anesthetized with anesthesia and blood was collected from the abdominal aorta, Blood was collected by centrifugation at 3,000 rpm for 15 minutes, and the serum of the upper layer was separated. The albumin was extracted with the separated serum. The amount of albumin extracted was measured using a commercially available kit, and the results are shown in FIG.

As shown in FIG. 3, in the case of Example 2-1, Example 2-2, and Example 2-3, it was confirmed that the amount of albumin was increased compared to the normal group and Comparative Example 1 and Comparative Example 2. Therefore, it was confirmed that the consumption of the extracts of roots, leaves or stems of the hairy budgie leaves the content of albumin produced by the liver high despite the consumption of alcohol. These results indicate that albumin is actively produced in spite of ingesting a simple alcohol, meaning that the liver function is recovered by ingesting roots, leaves or stem extracts of Brassica juncea, and thus has an efficacy for hangover relief.

< Experimental Example  4: LDL  Determination of cholesterol content>

In each group of rats according to Example 2-1, Example 2-2, Example 2-3, normal group, Comparative Example 1 or Comparative Example 2, each rat was anesthetized with ether and blood was collected from the abdominal aorta The collected blood was kept at refrigeration temperature for 30 minutes, centrifuged at 3,000 rpm for 15 minutes, and the serum of the upper layer was separated. The amount of total cholesterol, HDL and triglyceride content was measured using a commercially available assay kit. The content of LDL cholesterol was determined by the method of Friedewald et al. (1972) And HDL-cholesterol and triglyceride, respectively. The measurement results are shown in Table 3 below.

LDL cholesterol is a cholesterol that causes arterial sclerosis, which is deposited on the blood vessel wall. It is a cholesterol that affects our body badly, unlike HDL cholesterol which has a good effect on our body. Table 3 shows the range of the maximum amount and the minimum amount with respect to the average value in addition to the average value of LDL cholesterol. Even when the results of Examples 2-1, 2-2, and 2-3 are higher than those of the normal control, the LDL cholesterol transfer rate is higher than that of Comparative Example 1 and Comparative Example 2, It can be confirmed that it is remarkably lowered. Therefore, it can be confirmed that the ingestion of the root, leaf or stem extract of Brugaceae significantly reduces the amount of intra-arterial LDL cholesterol despite the consumption of alcohol. Thus, in Example 2-1, Example 2-2, and Example 2-3, it was confirmed that the extracts of roots, leaves or stems of Brassica juncea have an effect of preventing arteriosclerosis caused by alcohol.

< Experimental Example  5: Hairy Buddha  Root extract One time  &Lt; tb &gt; &lt;

In the single-dose administration of the hairy root extract of Example 4, an experiment was conducted to measure its efficacy

Thus, the activities of ADH and ALDH were measured in the same manner as in Experimental Example 1, and the amount of total glutathione was measured in the same manner as in Experimental Example 2 above. The amount of serum albumin was measured in the same manner as in Experimental Example 3, and the amount of HDL cholesterol was measured in the same manner as in Experimental Example 4. Experimental results were expressed as average and deviation for six rats except for the maximum and minimum values. The results are shown in Figs. 4 to 9. The mean values of the different alphabetic characters in the superscripts were significant at the p <0.05 level when the one way ANOVA test and the Duncan's multiple range test were performed.

In general, Hovenia dulcis extract is widely known as an extract useful for hangover relief. However, as shown in FIG. 4 and FIG. 5, the ADH and ALDH values measured after the administration of the same amount were the same as those of Comparative Example 3 The amount of extract was higher than that of single administration. 6, the amount of total glutathione was higher in the case of single administration of the extract of Example 4 than in the case of Comparative Example 3, and the value of oxidized glutathione / total glutathione was lower than that of Comparative Example 3, have. In FIG. 8, the amount of albumin in serum was larger than that in Comparative Example 3 in Example 4. Also in FIG. 9, it was confirmed that the amount of HDL cholesterol, which is a good cholesterol, was higher in Example 4 than in Comparative Example 3. As shown in FIGS. 4 to 9, the amount of ADH, ALDH, total glutathione, albumin, and HDL cholesterol was higher when the extract of Example 4 was administered than that of Comparative Example 4. The results of Experimental Example 6 demonstrate that root extract of Brassica japonica according to Example 4 is more effective in eliminating hangover after alcohol consumption than in Comparative Example 3 and Comparative Example 4. [

Therefore, as can be seen from Examples 2 to 4, the composition comprising the root, leaf and stem extract of the hairy flower of the present invention as an active ingredient and the beverage, medicine, And to prevent arteriosclerosis due to alcohol. Especially, the root extract of Fusarium oxyspora can be confirmed as a safe extract without genetic abnormality in our body even after ingestion, and it can be confirmed that the effect of hangover removal is excellent even in a single administration.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the appended claims. It is natural.

Claims (5)

Characterized in that it contains, as an active ingredient, an extract of at least one member selected from the group consisting of roots, leaves and stems of hairy buds, water, alcohol or a mixture thereof, and promotes the activity of aldehyde dehydrogenase (ALDH) Hangover. The method according to claim 1,
Wherein the extract is contained in an amount of 0.0001 to 99.9999% by weight.
delete A hangover beverage comprising the composition for hangover as claimed in claim 1 or 2. A hangover nutritional supplement comprising the composition for hangover as claimed in claim 1 or 2.

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