KR101428977B1 - microfluidic analysis device - Google Patents

Abstract

The present invention relates to a microfluidic analysis device that is capable of performing immunoassay by using a body fluid. More specifically, the microfluidic analysis device includes an inlet zone where a labeled ligand is stored and a fluid sample is injected; a reaction zone that is capable of reacting with at least one fixed target ligand or a zygote; and a waste zone. The microfluidic analysis device according to the present invention allows whole blood to be moved from the inlet zone to the waste zone by using a capillary phenomenon, and no sample remains in the reaction zone. In an analysis method for the microfluidic analysis device, the fluid is moved by the average of Laplace pressure difference between the inlet zone and the waste zone. A pressure gradient is calculated with a distance between the inlet zone and the waste zone. The amount of the ligand remaining without participating in the reaction can be checked accurately when no fluid sample remains in the reaction zone. Presence of particles present on a surface other than the reaction zone allows a signal-to-noise ratio to increase and signal distortion to decrease.

Description

≪ RTI ID = 0.0 > microfluidic <

The present invention relates to a microfluidic analysis apparatus capable of performing an immunoassay using a body fluid.

In recent years, efforts to simplify diagnostic tests have continued to increase. So far, the advantages and disadvantages of many different methods and devices have been suggested, among which immunoassay is one of the promising methods. Immunoassays use a ligand capable of reacting with the analyte and must be able to provide a detection signal after the reaction. For example, there is an antigen-antibody reaction, in which a second antibody is immobilized on a detection region that detects a signal of the labeled antibody and the antigen-antibody conjugate conjugated with the target antigen so as to be able to detect a reaction signal, Antibody conjugate to the immobilized second antibody and then detecting it. It is known that the above-mentioned platform for immunoassay comprises a plurality of holes and a microchannel, which are made of glass or plastic.

Detection of a false conjugation of the conjugated secondary antibody is very important because false signal detection causes the accuracy of diagnosis to be lowered. Therefore, in the conventional diagnostic kits or diagnostic chips, when blood is used, white blood cells having a large cell size and plasma other than red blood cells are used for diagnostic analysis. When the cell size includes large white blood cells or red blood cells, both the light and the fluorescence signal are blocked, reflected and deflected, and the noise ratio is increased, so that signal detection is not easy. Thus, not only is there an inconvenience of including additional steps such as a filter step to use or separate the separated blood, but also a washing step is required to remove samples not participating in the reaction using a large amount of blood or an additional solvent .

Accordingly, the present invention provides a microfluidic analytical instrument capable of increasing the signal-to-noise ratio by eliminating all of the sample in the detection portion and removing it from the waste portion.

Korean Patent Publication No. 2002-0054926 Korea Patent Publication No. KR 2012-0122209

It is an object of the present invention to provide a microfluidic analysis apparatus capable of performing immunoassay using body fluids.

More specifically, when a cell having a large size such as a blood cell is included, it is difficult to accurately detect it due to an increase in the noise ratio. Thus, the blood is separated and immunoassay using plasma is performed. And the whole blood can be used as it is.

In order to accomplish the above object, the present invention provides an injection apparatus including an injection unit including an upper substrate and a lower substrate, the injection unit including a first ligand capable of moving in combination with a target reaction product in a sample; A reaction unit including an immobilized second ligand capable of binding with the target reactant; And a waste part collecting the sample having passed through the reaction part, wherein the injection part, the reaction part and the waste part may be a microfluidic analysis device having a channel through which a fluid flows.

In the microfluidic analysis apparatus according to the present invention, the upper substrate and the lower substrate may be spaced apart from each other, and the separation distance may be uniformly reduced in the direction of the injection unit from the direction of the waste.

In the microfluidic analysis apparatus according to the present invention, the upper substrate and the lower substrate may be a microfluidic analyzer having an angle greater than 0 degrees and less than 1.5 degrees.

In the microfluidic analysis apparatus according to the present invention, the microfluidic analysis apparatus may be a microfluidic analysis apparatus in which the separation distance between the upper substrate and the lower substrate is reduced once and then uniformly decreased.

The microfluidic analysis apparatus according to the present invention may further include a static portion for allowing the fluid moving from the injection portion to the waste portion to stay for a predetermined period.

The microfluidic analysis apparatus according to the present invention may be a microfluidic analysis apparatus in which all the fluid in the injection unit moves to the waste part.

In the microfluid analysis apparatus according to the present invention, the upper substrate and the lower substrate may have a rectangular shape, and may be a microfluid analyzer having a thickness of 0.5 to 5 mm, a length of 20 to 150 mm, and a width of 10 to 60 mm .

In the microfluidic analysis apparatus according to the present invention, the material of the upper substrate and the lower substrate may be any one selected from a polymer, metal, and glass.

In the microfluidic analysis apparatus according to the present invention, the first ligand may be a microfluidic analysis apparatus, which is a first antibody labeled with a target specific fluorescent substance.

In the microfluidic analysis apparatus according to the present invention, the second ligand may be a microfluidic analysis apparatus, which is a second antibody labeled with a target specific fluorescent substance immobilized on a lower substrate.

In the microfluidic analysis apparatus according to the present invention, the channel of the waste section may be a microfluidic analysis apparatus having a circular or linear groove.

In the microfluid analysis apparatus according to the present invention, the upper substrate or the lower substrate may be a microfluidic analysis apparatus coated with a hydrophobic substance or a hydrophilic substance.

In the microfluid analysis apparatus according to the present invention, when the sample is hydrophilic, the injection section of the upper substrate or the lower substrate may be coated with the hydrophobic substance, and the waste section may be a microfluid analysis device coated with the hydrophilic substance .

In the microfluid analysis apparatus according to the present invention, when the sample is hydrophobic, the injection portion of the upper substrate or the lower substrate may be coated with the hydrophilic material, and the waste portion may be a microfluidic analysis device coated with the hydrophobic material .

In the microfluidic analysis apparatus according to the present invention, the hydrophobic substance may be any one selected from the group consisting of hydroxyl carbon, polytetrafluoroethylene, and hydrophobic inks containing three or more alkyl groups, Lt; / RTI >

In the microfluidic analysis apparatus according to the present invention, the hydrophilic material may be at least one selected from the group consisting of titanium oxide, aluminum oxide, chromium oxide coating, plasma, It may be an apparatus for analyzing microfluid, which is selected.

In the microfluidic analysis apparatus according to the present invention, the sample may be a microfluidic analysis apparatus which is a bodily fluid.

The present invention relates to an apparatus for analyzing microfluidic fluids using whole blood. The present invention relates to an apparatus for analyzing microfluidic fluids by using whole blood without separating blood, and a step of washing or separating It is possible to provide a microfluidic analysis apparatus which is capable of not only a very convenient and simple diagnosis analysis but also a signal-to-noise ratio can be increased and signal detection limit is very low and sensitivity of detection is high.

In addition, since it is possible to detect even a small amount of sample, the cost of the diagnostic test can be reduced, and the analyzer can perform a simple and convenient diagnostic test.

1 is a schematic diagram of a microfluidic analysis apparatus.
2 is a schematic view showing a slope between two substrates in a channel structure in a reaction part.
3 is a schematic view of an injection unit, (a) a schematic view of an injection unit in which a plurality of channels are formed, and (b) a schematic view of an injection unit in which a dual channel is formed;
FIG. 4 is a schematic view showing a flat structure in which (a) a channel is not formed in a waste part, (b) a schematic view showing a waste part formed with a linear channel, and (c) a waste part formed with a circular channel.
[Description of Symbols]
1: inlet zone
2: reaction zone
3: waste zone

The present invention relates to a microfluidic analysis apparatus using whole blood. The present invention provides an apparatus comprising: an injector comprising an upper substrate and a lower substrate, the injector including a first ligand capable of moving in combination with a target reactant in the sample; A reaction unit including an immobilized second ligand capable of binding with the target reactant; And a waste part collecting the sample having passed through the reaction part, wherein the injection part, the reaction part and the waste part may be a microfluidic analysis device having a channel through which a fluid flows.

 In addition, the lower substrate and the upper substrate may be spaced apart from each other at an angle that is constantly decreasing continuously from the injection unit toward the waste part, And can be reduced constantly in the negative direction.

 Thus, the vertical distance between the upper substrate and the lower substrate in the waste portion may be shorter than the vertical distance between the lower substrate and the upper substrate in the implant.

The capillary force varies depending on the degree of separation between the upper substrate and the lower substrate, and the flow rate of the microfluid, which is a sample, varies depending on the capillary force.

Therefore, by controlling the degree of separation, it is possible to give sufficient time for the reaction to take place by controlling the flow rate.

In addition, the continuously decreasing spacing can be adjusted to slow the flow rate of the fluid up to the point where it reacts with the first and second ligands, and to increase the flow rate of the fluid in the waste section.

That is, in the microfluid analysis apparatus according to the present invention, the separation distance between the upper substrate and the lower substrate may be a microfluidic analysis apparatus in which the separation distance is reduced once and then uniformly decreased.

As described above, the upper substrate and the lower substrate according to the present invention are spaced apart from each other, and the spacing angle includes all the conditions for giving sufficient time for the first and second ligands to react with the target reactant in the sample in the injection unit and the reaction unit do.

That is, if the reaction angle is too small, it is impossible to control the flow rate, and if the reaction angle is too large, the reaction time between the target reactant and the first and second ligands in the sample will not be sufficiently given.

As one non-limiting example, the upper substrate and the lower substrate of the microfluidic analysis apparatus according to the present invention may have an angle of more than 0 degrees and less than 1.5 degrees, but are not limited thereto.

The microfluidic analysis apparatus according to the present invention may further include a stagnant portion for keeping the fluid moving from the injection portion to the waste portion for a predetermined period of time.

The stagnation part includes all the conditions for delaying the moving speed of the fluid by allowing the fluid moving from the injection part to the waste part to stay for a predetermined time and giving sufficient reaction time.

As a non-limiting embodiment, the stagnant portion may be formed by a method in which the capillary force acts on the fluid after filling a certain portion through separation of the channel, a method of stopping the fluid through the three-dimensional structure, Flow velocity, flow rate, and the like.

The shape of the upper or lower substrate according to the present invention includes all conditions under which the target reactant in the sample can react with the first ligand and the second ligand.

As a non-limiting example, the upper or lower substrate according to the present invention may have a rectangular shape with a thickness of 0.5 to 5 mm, a length of 20 to 150 mm and a width of 10 to 60 mm, And can be freely deformed according to the purpose of use and the amount of fluid sample.

The microfluidic analysis apparatus according to the present invention may be a microfluidic analysis apparatus in which all fluids including a target material in a sample to be dispensed into an injection section are moved to a waste section.

Therefore, according to the present invention, it is possible to increase the signal-to-noise ratio by allowing the sample to remain in the reaction part and to be removed from the reaction part.

Hereinafter, the structure of the injector, the reaction unit, and the waste unit according to the present invention will be described. However, the following description explains one example according to the present invention, and the technical idea of the present invention is not limited by the following description.

1. Inlet zone

The injector is a region where the fluid sample is dispensed, and is characterized in that the droplet of the fluid sample can be rapidly moved from the injection portion to the capillary space, which is the reaction portion, and the injected portion includes the labeled first ligand.

The label may include fluorescent particles or magnetic particles, and the first ligand may be selected from proteins, peptides, DNA, RNA, PNA, and the like, but is not limited thereto.

Preferably, the protein or peptide is selected from the group consisting of an antibody, an enzyme and a hormone, and DNA, RNA or PNA synthesized by a primer or a probe may be used. The preferred fluorescent material is any one selected from FAM, JOE, TET, HEX, VIC, DABCYL, TAMRA, ROX, and BHQ.

 The injection part can be coated with a material whose capillary force can be reduced since the fluid sample must move to the reaction part.

As a non-limiting example, hydrophobic coating materials include hydroxyl carbon, polytetrafluoroethylene, and hydrophobic inks containing three or more alkyl groups. Hydrophilic coating materials include titanium oxide, But are not limited to, aluminum oxide, chromium oxide coating, plasma, and oxidized film by Tesla discharge.

The coating material is used to control the rate at which the fluid moves from the injection part to the reaction part and the capillary force. Thus, the hydrophilic property and the hydrophobic property may be determined in consideration of the properties of the sample material.

Therefore, when the sample material is a hydrophilic material, a hydrophobic material may be coated to reduce the flow rate and capillary force at the injection part. When the sample material is hydrophobic, a hydrophilic material may be added to reduce the flow rate and capillary force at the injection part. Can be coated.

As a non-limiting example, when the sample material is a bodily fluid, the coating material in the injection portion may be selected from the group consisting of hydroxyl carbon, polytetrafluoroethylene, hydrophobic ink, etc. containing three or more alkyl groups as a hydrophobic coating material Lt; / RTI >

As shown in FIG. 3, the lower substrate according to the present invention includes one or a plurality of grooves having a depth of 0.1 mm to 4 mm.

2. Reaction zone

The reaction part is a capillary space between the injection part and the waste part, more specifically, a fine channel formed by the separation of the lower substrate and the upper substrate.

It is preferable that the depth of the microchannel of the reaction part is 0.1 탆 to 4 탆. In order to control the flow rate and the capillary force of the microfluidic sample flowing in the reaction part, the inner surface of the lower substrate or the upper substrate is made hydrophilic and hydrophobic Can be coated.

The hydrophobic substance means a substance which does not easily bind to water or a substance containing a functional group in the molecule, and the hydrophilic substance means a substance which is easily bound to water molecules.

As a non-limiting example, hydrophobic coating materials include hydroxyl carbon, polytetrafluoroethylene, and hydrophobic inks containing three or more alkyl groups. Hydrophilic coating materials include titanium oxide, But are not limited to, aluminum oxide, chromium oxide coating, plasma, and oxidized film by Tesla discharge.

In the reaction part, in order to sufficiently give a reaction time between the sample material and the second ligand, the hydrophilic and hydrophobic materials may be coated depending on the characteristics of the sample material.

As a non-limiting example, when the sample material is a bodily fluid, it may be coated with a hydrophobic substance to reduce the reaction time and the capillary-related relation, thereby giving sufficient time for the reaction.

Also, the lower substrate and the upper substrate are spaced apart from each other by an angle which is constantly decreasing continuously from the direction of the injection part to the direction of the waste part, so that the spacing of the injecting part is larger than the spacing of the waste part.

The reaction unit is characterized in that a second ligand, to which a labeling component such as fluorescent particles or magnetic particles capable of electronically or optically detecting the microfluidic sample, is bound, is immobilized in the reaction unit. The second ligand to which the labeling component such as the fluorescent substance or the magnetic particle is bound is selected from a protein or a peptide, DNA, RNA, or PNA, but is not limited thereto.

The protein or peptide may be selected from antagonists such as antibodies, enzymes or hormones. DNA, RNA or PNA synthesized as a primer or a probe may be used.

3. Waste zone

The capillary space between the two substrates may coat a material capable of increasing the capillary force, and the material capable of increasing the capillary force may be a hydrophobic coating material. Hydroxyl carbon, polytetrafluoroethylene, hydrophobic ink and the like containing at least one alkyl group may be used. Examples of the hydrophilic coating material include titanium oxide, aluminum oxide, chromium oxide oxide coating, a plasma, and an oxidation treatment film by a Tesla discharge, but the present invention is not limited thereto.

The coating material is used to control the speed at which the fluid moves in the waste part and the capillary force, so that the hydrophilic and hydrophobic materials may be coated or coated in consideration of the properties of the sample material.

Therefore, when the sample material is a hydrophilic material, a hydrophilic material may be coated to increase the flow rate and capillary force at the waste part. If the sample material is hydrophobic, a hydrophobic substance may be added to increase the flow rate and capillary force at the injection part. Can be coated.

As a non-limiting example, if the sample material is a bodily fluid, the coating material in the waste portion may be a titanium oxide, an aluminum oxide, a chromium oxide coating, a plasma, An oxide film formed by a Tesla discharge, or the like.

As shown in FIG. 4, the waste portion may have a flat structure without a channel, a channel with a linear pattern structure, or a circular structure.

The standard of the linear and circular channels includes all the shapes for achieving the object of the present invention. As a non-limiting example, in the case of the linear patterned channel, the separation distance between channels is 100 탆 to 5 mm, The diameter of the circular shape is 100 mu m to 3 mm, and the separation distance between the circular patterns is preferably 300 mu m to 8 mm, but is not limited thereto.

In addition, the linear or circular channel structure roll patterning depth includes all conditions that prevent fluid from flowing to the substrate due to surface tension, and as a non-limiting example, the patterning depth of the channel is at least 50 탆 . When patterning is performed at a depth of less than 50 탆, the fluid tends to flow due to the surface tension, which is not preferable.

4. Static zone

 The stagnation part includes all the structures having a structure in which the moving speed of the fluid is delayed during a certain period of time when the fluid moves from the direction of the injection part to the direction of the waste part.

That is, in the microfluidic analysis apparatus according to the present invention, the stagnant portion delays the moving speed of the fluid to give sufficient time for the reaction.

In order to attain the object of the stagnation part, a material capable of reducing the capillary force may be coated, and the material capable of reducing the capillary force may be a hydrophobic coating material such as three or more of alkyl groups such as hydroxyl carbon, And hydrophobic coating materials such as titanium oxide, aluminum oxide, chromium oxide coating, plasma, and oxidation treatment by Tesla discharge. Examples of the hydrophilic coating material include titanium oxide, aluminum oxide, chromium oxide coating, Films, and the like, but are not limited thereto.

As described above, the present invention provides an injection apparatus including an injection unit including an upper substrate and a lower substrate, the injection unit including a first ligand capable of moving in combination with a target reaction product in a sample; A reaction unit including an immobilized second ligand capable of binding with the target reactant; And a waste part collecting the sample having passed through the reaction part, wherein the injection part, the reaction part and the waste part may be a microfluidic analysis device having a channel through which a fluid flows.

Further, in the microfluidic analysis apparatus according to the present invention,

In the microfluid analysis apparatus according to the present invention, the injection unit includes a labeled first ligand, and the reaction unit includes a second ligand immobilized thereon, and is a place for sensing a signal. In the present invention, the flow velocity is characterized by using Laplace pressure. The Laplacian pressure is generated by the surface tension of the interface between the liquid and the gas, which means the pressure difference inside or outside the bubble or droplet. The capillary force (force, F) generated in the capillary can be expressed by the following equation (1)

[Equation 1]

 In Equation (1), FC is the capillary force of the channel surface, and FL is the force generated by the Laplace pressure.

In addition, the remaining meniscus capillary force Fc1 is expressed by the following equation (2).

The meniscus refers to the coplanar surface formed by the liquid surface in the capillary due to the capillary phenomenon, and the liquid surface becomes concave or convex depending on the properties of the liquid.

&Quot; (2) "

In Equation (2), r1 is the channel radius and silver is the surface tension.

The value for the pressure P1 in the following equation (3) can be obtained in the meniscus.

&Quot; (3) "

Where S1 is the area of the small meniscus, the direction of the capillary force is in the opposite direction of the fluid flow, and the direction of the Laplace force is in the same direction as the fluid flow, and therefore in different directions. Therefore, the capillary force can be expressed by the following equation (4).

&Quot; (4) "

Thus, the remaining smaller capillary force meniscus can be expressed as: " (5) "

&Quot; (5) "

The pressure (P) generated on the concave surface by the Laplace is expressed by the following equation (6).

&Quot; (6) "

Where P is the pressure differential across the fluid interface, is the surface tension, and R is the meniscus radius.

2, the pressure P1 of the meniscus shown on the left side can be expressed by the following equation (7), and the pressure P2 of the small meniscus on the right side can be expressed by the following equation (8).

&Quot; (7) "

&Quot; (8) "

The total pressure P in the entire droplet according to the Pascal's pressure law can be expressed by the following equation (9), which is obtained by adding P1 and P2.

&Quot; (9) "

The force acting at the left and right ends of the moving body (microfluid) can be expressed by the following equation (10).

&Quot; (10) "

및

.

And

.

Therefore, the following equations (11) to (13) are derived from the equations (1) to (10).

&Quot; (11) "

&Quot; (12) "

&Quot; (13) "

F = P2S1 - P1S2

When the angle between the channel central axis and the channel wall is the tilting angle, the force F can be expressed by the following equation (14).

&Quot; (14) "

Therefore, since R1 is larger than R2, the force pushes the microfluid in the narrow direction of the channel and moves from the left side (injection side) to the right side (reaction side).

The microfluidic fluid that can be used in the present invention is a body fluid, and the body fluid may be any one selected from blood, urine, tears, mucus, and needle. More preferably, the body fluid is blood, and the blood is any one selected from whole blood, plasma, red blood cells, and white blood cells, and the most preferable sample is whole blood.

In the microanalysis analyzing apparatus according to the present invention, the upper substrate and the upper substrate include all materials for achieving the object of the present invention.

As a non-limiting example, the material of the upper and lower substrates according to the present invention is preferably made of any one selected from a polymer, metal, or glass, but is not limited thereto.

The polymer may be selected from the group consisting of polymethylmethacrylate, polycarbonate, polystyrene, polypropylene, polyethylene, polyester, chlorine-containing polymers, acrylic polymers, polytetrafluoroethylene More preferably selected from polymers such as polymethyl methacrylate, polytetrafluoroethylene and the like, and the metal is more preferably selected from aluminum, anodized aluminum and steel.

In addition to the above-mentioned materials, the constituent components of the apparatus can be coated on the polymer, plated or patterned, or treated with chemicals to obtain surface energy.

In the microfluidic analysis apparatus according to the present invention, the upper substrate or the lower substrate may be coated with a hydrophilic or hydrophobic material to form the capillary force.

The hydrophilic material or the hydrophobic material to be coated is for increasing or decreasing the capillary force. When the measurement sample according to the present invention is hydrophilic, the injection part of the upper substrate or the lower substrate is coated with the hydrophobic material, May be a microfluidic analyzer in which the hydrophilic material is coated.

In addition, when the measurement sample according to the present invention is hydrophobic, the injection portion of the upper substrate or the lower substrate may be coated with the hydrophilic material, and the waste portion may be a microfluidic analysis device coated with the hydrophobic material.

The microfluidic analysis apparatus according to the present invention can be used conveniently without separating the whole blood as the blood itself, so that it can be very conveniently examined.

In addition, the microfluidic analysis apparatus of the present invention does not require a step of washing the analyte from the reaction zone after the reaction.

The microfluidic analysis apparatus of the present invention includes a capillary space between two substrates, and the capillary space is determined by the surface tension or the slope of the microchannel. The capillary space is formed on at least one of the substrates .

The capillary space includes hydrophilic and hydrophobic regions patterned on one substrate surface and is formed by surface tension.

The apparatus of the present invention is characterized in that the fluid is moved by a capillary force and the capillary pressure is selected from the group consisting of (1) hydrophilic and / or hydrophobic characteristics of the substrate surface, (2) area and (3) the angle between the two substrates. The tilt angle of the substrate or the slope of the microchannel provides a capillary pressure differential at the injection and discard sections. Since the height of the capillary space in the injection part is higher than the height in the waste part, the capillary pressure of the injection part is lower than the capillary pressure of the waste part and the direction of the fluid flow flows from the injection part to the waste part.

Immunoassay is an assay method for measuring a desired small amount of a substance from a biological sample containing a large amount of impurities by using a high degree of specificity between an antigen and an antibody. Thus, a preferred example of the present invention is an antigen-antibody binding reaction.

Claims (17)

An upper substrate and a lower substrate,
An injector including a first ligand capable of moving in association with a target reactant in a sample;
A reaction unit including an immobilized second ligand capable of binding with the target reactant;
And a waste part collecting the sample having passed through the reaction part,
Wherein the injection unit, the reaction unit, and the waste unit have a channel through which a fluid flows. The method according to claim 1,
Wherein the upper substrate and the lower substrate are spaced apart from each other and the spacing distance is reduced uniformly in the direction of the discard portion from the direction of the injection portion. The method according to claim 1,
Wherein the upper substrate and the lower substrate form an angle of more than 0 degrees and less than 1.5 degrees. 3. The method of claim 2,
Wherein the spacing distance is reduced by one time and decreased uniformly. 3. The method of claim 2,
And a stagnant part for allowing the fluid moving from the injection part to the waste part to stay for a predetermined period. 3. The method of claim 2,
Wherein all the fluids in the injection section are moved to the waste section. 3. The method of claim 2,
Wherein the upper substrate and the lower substrate have a rectangular shape with a thickness of 0.5 mm to 5 mm, a length of 20 mm to 150 mm, and a width of 10 mm to 60 mm. 3. The method of claim 2,
Wherein the upper substrate and the lower substrate are made of a material selected from the group consisting of a polymer, a metal, and glass. The method according to claim 1,
Wherein the first ligand is a first antibody labeled with a target specific fluorescent substance. The method according to claim 1,
Wherein the second ligand is a second antibody labeled with a target specific fluorescent substance immobilized on a lower substrate. The method according to claim 1,
Wherein the channel of the waste part comprises a circular or linear groove. The method according to claim 1,
Wherein the upper substrate or the lower substrate is coated with a hydrophobic substance or a hydrophilic substance. 13. The method of claim 12,
Wherein the injection unit of the upper substrate or the lower substrate is coated with the hydrophobic substance when the sample is hydrophilic and the hydrophilic substance is coated with the waste unit. 13. The method of claim 12,
Wherein when the sample is hydrophobic, the injection portion of the upper substrate or the lower substrate is coated with the hydrophilic material, and the waste portion is coated with the hydrophobic material. 13. The method of claim 12,
Wherein the hydrophobic substance is any one selected from the group consisting of hydroxyl carbon, polytetrafluoroethylene, and hydrophobic inks containing three or more alkyl groups. 13. The method of claim 12,
Wherein the hydrophilic material is one selected from the group consisting of titanium oxide, aluminum oxide, chromium oxide coating, plasma, and oxidation treatment film by Tesla discharge. The method according to claim 1,
Wherein the sample is a bodily fluid.

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