KR101427180B1 - OsCTR1 gene from rice for increasing drought stress resistance of plant and uses thereof - Google Patents

Abstract

The present invention provides a method for controlling environmental stress resistance of a plant, comprising a step of transforming a recombination vector including Oryza sativa chloroplast proteins targeting RING E3 ligase 1 (OsCTR1) gene derived from a rice plant into a plant cell; a method for manufacturing a transgenic plant with regulated environmental stress resistance, comprising a step of transforming the plant cell with the recombination vector including the OsCTR1 gene; a transgenic plant produced by the method, in which environmental stress resistance is regulated; and a composition for regulating environmental stress resistance including the recombination vector including the OsCTR1 gene and seeds of a transgenic plant.

Description

(OsCTR1 gene from rice for increasing stress resistance of plant and uses thereof)

The present invention relates to rice-derived OsCTR1 gene and its use for increasing drought stress tolerance of a plant, and more particularly, to a rice-derived OsCTR1 gene ( Oryza the sativa chloroplast proteins targeting RING E3 ligase 1) a plant cell by a method for controlling the resistance to environmental stress in plants, the recombinant vector containing the gene comprising the step of transforming a plant cell, a recombinant vector including the OsCTR1 gene A method for producing a transgenic plant having a tolerance to environmental stress, comprising the step of transforming, a transgenic plant having a tolerance to environmental stress produced by the method, a seed thereof, and the OsCTR1 gene To a composition for controlling environmental stress tolerance of a plant containing a recombinant vector as an active ingredient.

Studies on the defense mechanism of plants with resistance to plant disease resistance or environmental stress have technological value to identify in vivo mechanisms not yet known. In addition, information on plant defense genes can be used not only as a direct material for molecular genetic breeding of crops, but also for traditional genetic breeding.

In terms of environmentally friendly food production, various genes related to disease defenses, or crop breeding using these traits, can reduce food production costs by minimizing losses caused by economically important pathogens, weeds, and environmental stresses, Contributing to the recovery of agricultural products' competitiveness and contributing to the revitalization of various industries associated with the development of new varieties.

Environmental stresses are water stress, cold stress, salt stress, and oxidative stress, which inhibit plant growth and limit the productivity of crops in many important agricultural fields. Particularly, water stress is caused by external environment such as dehydration, high saltiness and low temperature, and is one of the most severe stresses in environmental stress which inhibits plant growth.

Plants have a defense mechanism against such water stress, and when they are in an environment unsuitable for growth, they tend to adjust to their environment or adjust their physiological metabolic processes to survive in the environment. So far, many genes involved in water stress have been isolated.

Korean Patent No. 0792169 discloses a plant having enhanced photosensitization, delayed flowering and resistance to environmental stress through gene transfer of the transcription factor AtMYB44, and Korean Patent No. 0742194 discloses a plant having an environmental stress resistance regulatory gene Discloses a method for increasing environmental stress resistance of a plant. However, as described in the present invention, the OsCTR1 gene derived from rice and its use for increasing the drought stress tolerance of a plant has not been disclosed.

DISCLOSURE OF THE INVENTION The present invention has been made in view of the above needs, and the present inventors have found that the OsCTR1 gene derived from rice is strongly induced in drought stress, is located in cytoplasm and chloroplasts of intracellular cells and mainly interacts with proteins located in chloroplasts And OsCTR1 gene was overexpressed in the Arabidopsis thaliana plants, and showed that OsCTR1 is an important gene for environmental stress. Thus, the present invention has been completed.

In order to solve the above-mentioned problems, the present invention relates to a method for producing rice from OsCTR1 ( Oryza sativa chloroplast proteins targeting RING E3 ligase 1) a method for regulating tolerance to environmental stress of a plant comprising transforming a recombinant vector containing the gene into a plant cell.

In addition, the present invention provides a method for producing a transgenic plant having resistance to environmental stress, which comprises transforming a plant cell with a recombinant vector comprising the OsCTR1 gene.

In addition, the present invention provides a transgenic plant having the tolerance to environmental stress produced by the above method.

The present invention also provides a seed of the plant.

In addition, the present invention provides the above OsCTR1 A composition for controlling environmental stress tolerance of a plant containing a recombinant vector containing a gene as an active ingredient.

Through the present invention, it was confirmed that OsCTR1 gene-overexpressing plants showed a stronger resistance to drought stress treatment than the control group. Therefore, the OsCTR1 gene is expected to be useful for the development of transgenic plants resistant to environmental stress.

In addition, it is possible to provide OsCTR1 as a basic knowledge in related fields by analyzing the intracellular location and interaction protein with OsCTR1.

FIG. 1 shows the results of analysis of the expression pattern of OsCTR1 gene by RT-PCR using tissues such as roots, stems, leaves, and panicles and spikes of rice plants. A) The expression pattern of OsCTR1 gene in different tissues and other developmental stages of the plant was confirmed, showing that there is a difference in the expression pattern of genes in each tissue and each stage of development. Stage I is a nutritional growth stage, Stage II is a reproductive stage. B) it is an illustration showing that the abiotic stress and to confirm the expression pattern of the gene according to OsCTR1 dehydration stress and plant hormone treatment plant expression induction of the gene OsCTR1 during hormone treatment is confirmed. C is a control group and T is a stress-treated group.
Figure 2 is a OsCTR1 results of testing the gene expression level for an independent third object of the transgenic Arabidopsis thaliana plants by inducing over-expression of OsCTR1 gene by RT-PCR method. WT is a wild-type Arabidopsis; 35S: EYFP is a transgenic Arabidopsis transformed with a recombinant vector not containing the OsCTR1 gene; 35S: OSCTR1-EYFP # 1, 4, 5 are transgenic Arabidopsis thaliana.
FIG. 3 shows the ABA sensitivity of transformed Arabidopsis thaliana 35S (OSCTR1-EYFP # 1, 4, 5) overexpressing the OsCTR1 gene. As a result of culturing in a medium containing 1 μM ABA, (35S: EYFP) Arabidopsis thaliana, the transgenic Arabidopsis overexpressing OsCTR1 gene was found to be highly susceptible to ABA. The bar size is 1 cm.
FIG. 4 is a diagram showing a phenotypic analysis of the transgenic and non-transformed Arabidopsis thaliana ABA hormone overexpressing the OsCTR1 gene. A) The germination rate of each ABA treatment concentration was confirmed. The germination rate of the transgenic plants was lower than that of the non-transgenic plants. B) Was lower than that of the control group.
FIG. 5 shows the results obtained by examining the degree of pore opening and closing of each of the transgenic Arabidopsis thaliana (35S: OSCTR1-EYFP # 1, 4) and non-transformed Arabidopsis thaliana by ABA concentration and treating ABA hormone at a concentration of 1, 10 μM This is a photograph and a graph comparing the degree of pore opening and closing by an optical microscope.
FIG. 6 shows the result of observation of the leaves of transgenic and non-transformed Arabidopsis thaliana during natural drying. A) is a measurement of the weight change due to water spillage, which shows that the transgenic plants have improved moisturizing properties, and B) is a photograph showing the leaf shape changes after 24 hours and 48 hours in this state.
FIG. 7 shows the results of measurement of hydrogen peroxide (H ₂ O ₂ ) accumulation in transformed and non-transformed Arabidopsis thaliana leaves. The degree of hydrogen peroxide production in the leaves 90 minutes and 180 minutes after dehydration was analyzed by histochemical staining It's a picture.
FIG. 8 is a photograph showing the result of stopping water supply for 10 days in transgenic and non-transformed Arabidopsis thaliana grown for 2 weeks and resistance to drought stress of the plant and showing the survival rate after 3 days of water supply.

In order to accomplish the object of the present invention, the present invention provides a method for producing a plant resistant to environmental stress, comprising the step of transforming plant cells with a recombinant vector comprising OsCTR1 ( Oryza sativa chloroplast proteins targeting RING E3 ligase 1) The method comprising the steps of:

The method according to one embodiment of the present invention is characterized in that rice-derived OsCTR1 The present invention is characterized by overexpressing a recombinant vector containing a gene in a plant cell to increase tolerance to environmental stress of the plant, but is not limited thereto.

In the present invention, "environmental stress" refers to an external factor that lowers the growth or productivity of a plant, and is roughly divided into biotic stress and abiotic stress. Examples of biological stresses include pathogens. Abiotic stresses include high concentrations of salt, drying, low temperature, high temperature, and oxidative stress. The term " environmental stress tolerance "refers to a trait that suppresses or delays plant growth or productivity deterioration due to environmental stress.

In the method according to an embodiment of the present invention, the environmental stress may be, but is not limited to, drought stress.

In the method according to one embodiment of the present invention, the OsCTR1 gene may preferably consist of the nucleotide sequence of SEQ ID NO: 1. In addition, homologues of the nucleotide sequences are included within the scope of the present invention. Specifically, the gene has a nucleotide sequence having a sequence homology of 70% or more, more preferably 80% or more, still more preferably 90% or more, and most preferably 95% or more, with the nucleotide sequence of SEQ ID NO: 1 . "% Of sequence homology to polynucleotides" is ascertained by comparing the comparison region with two optimally aligned sequences, and a portion of the polynucleotide sequence in the comparison region is the reference sequence for the optimal alignment of the two sequences (I. E., A gap) relative to the < / RTI >

The term "recombinant" refers to a cell in which a cell replicates a heterologous nucleic acid, expresses the nucleic acid, or expresses a protein encoded by a peptide, heterologous peptide or heterologous nucleic acid. The recombinant cell can express a gene or a gene fragment that is not found in the natural form of the cell in one of the sense or antisense form. In addition, the recombinant cell can express a gene found in a cell in its natural state, but the gene has been modified and reintroduced intracellularly by an artificial means.

In the present invention, the OsCTR1 The gene sequence may be inserted into a recombinant expression vector. The term "recombinant expression vector" means a bacterial plasmid, a phage, a yeast plasmid, a plant cell virus, a mammalian cell virus, or other vector. In principle, any plasmid and vector can be used if it can replicate and stabilize within the host.

Expression vectors containing OsCTR1 gene sequences and appropriate transcription / translation control signals can be constructed by methods known to those skilled in the art. Such methods include in vitro recombinant DNA technology, DNA synthesis techniques, and in vivo recombination techniques. The DNA sequence can be effectively linked to appropriate promoters in the expression vector to drive mRNA synthesis. The expression vector may also include a ribosome binding site and a transcription terminator as a translation initiation site.

A preferred example of the recombinant vector of the present invention is a Ti-plasmid vector capable of transferring a so-called T-region to a plant cell when present in a suitable host, such as Agrobacterium tumefaciens. Other types of Ti-plasmid vectors (see EP 0 116 718 B1) are currently used to transfer hybrid DNA sequences to plant cells or protoplasts in which new plants capable of properly inserting hybrid DNA into the plant's genome can be produced have. A particularly preferred form of the Ti-plasmid vector is a so-called binary vector as claimed in EP 0 120 516 B1 and U.S. Patent No. 4,940,838. Other suitable vectors that can be used to introduce the DNA according to the invention into the plant host include viral vectors such as those that can be derived from double-stranded plant viruses (e. G., CaMV) and single- For example, from non -complete plant virus vectors. The use of such vectors may be particularly advantageous when it is difficult to transform the plant host properly.

The expression vector will preferably comprise one or more selectable markers. The marker is typically a nucleic acid sequence having a property that can be selected by a chemical method, and includes all genes capable of distinguishing a transformed cell from a non-transformed cell. Examples include herbicide resistance genes such as glyphosate or phosphinothricin, antibiotics such as kanamycin, G418, Bleomycin, hygromycin, chloramphenicol, Resistant gene, aadA gene, and the like, but are not limited thereto.

In the recombinant vector of the present invention, the promoter may be CaMV 35S, actin, ubiquitin, pEMU, MAS, histone promoter, Clp promoter, but is not limited thereto. The term "promoter " refers to the region of DNA upstream from the structural gene and refers to a DNA molecule to which an RNA polymerase binds to initiate transcription. A "plant promoter" is a promoter capable of initiating transcription in plant cells. A "constitutive promoter" is a promoter that is active under most environmental conditions and developmental conditions or cell differentiation. Constructive promoters may be preferred in the present invention because the choice of transformants can be made by various tissues at various stages. Thus, constitutive promoters do not limit selectivity.

In the recombinant vector of the present invention, conventional terminators can be used. Examples thereof include nopaline synthase (NOS), rice α-amylase RAmy1 A terminator, phaseoline terminator, Agrobacterium tumefaciens ( Agrobacterium tumefaciens ) Terminator of the Octopine gene, and the rrnB1 / B2 terminator of E. coli, but the present invention is not limited thereto. Regarding the need for terminators, it is generally known that such regions increase the certainty and efficiency of transcription in plant cells. Therefore, the use of a terminator is highly desirable in the context of the present invention.

When the vector of the present invention is transformed into eukaryotic cells, yeast ( Saccharomyce cerevisiae), and the like insect cells, human cells (e.g., CHO cells (Chinese hamster ovary), W138, BHK, COS-7, 293, HepG2, 3T3, RIN and MDCK cell lines) and plant cell may be used. The host cell is preferably a plant cell.

The method of delivering the vector of the present invention into a host cell can be carried out by injecting a vector into a host cell by microinjection, calcium phosphate precipitation, electroporation, liposome-mediated transfection, DEAE-dextran treatment, can do.

The present invention also relates to a method for producing rice from OsCTR1 ( Oryza sativa chloroplast proteins targeting a plant cell with a recombinant vector containing the RING E3 ligase 1) gene; And regenerating the plant from the transformed plant cell. The present invention also provides a method for producing a transgenic plant having a tolerance to environmental stress.

The method according to one embodiment of the present invention is characterized by overexpression of a recombinant vector comprising a rice-derived OsCTR1 gene in a plant cell to increase tolerance to environmental stress of the plant, but is not limited thereto.

Preferably, the OsCTR1 gene comprises the nucleotide sequence of SEQ ID NO: 1.

In the method according to an embodiment of the present invention, the environmental stress may be, but is not limited to, drought stress.

The method of the invention comprises the step of transforming a plant cell with a recombinant vector according to the present invention, the transformant is, for example, Agrobacterium tyumeo Pacific Enschede may be mediated by (Agrobacterium tumefiaciens). In addition, the method of the present invention comprises regenerating a transgenic plant from the transformed plant cell. Any of the methods known in the art can be used for regeneration of transgenic plants from transgenic plant cells.

Transformed plant cells must be regenerated into whole plants. Techniques for the regeneration of mature plants from callus or protoplast cultures are well known in the art for a number of different species (Handbook of Plant Cell Culture, Vol. 1-5, 1983-1989, Momillan, N. Y.).

In addition, the present invention provides transgenic plants and seeds thereof, which are prepared by the above method and whose tolerance to environmental stress is regulated.

Preferably, the transgenic plants and their seeds are transgenic plants and their seeds with increased resistance to environmental stress.

The plant may be selected from the group consisting of Arabidopsis, potato, eggplant, tobacco, red pepper, tomato, burdock, cilantro, lettuce, bellflower, spinach, modern sweet potato, celery, carrot, parsley, parsley, cabbage, cabbage, It may be a dicotyledonous plant such as squash, poultry, strawberry, soybean, mung bean, kidney bean or pea or a monocotyledon such as rice, barley, wheat, rye, corn, sorghum, oats and onion, But is not limited thereto.

The present invention also provides a method for producing a rice-derived OsCTR1 (RING E3 ligase 1) gene of Oryza sativa chloroplast proteins as an active ingredient. The present invention also provides a composition for controlling environmental stress tolerance of a plant. The above composition comprises OsCTR1 having the nucleotide sequence of SEQ ID NO: 1 as an active ingredient The present invention provides a recombinant vector containing a gene, which can increase environmental stress tolerance, preferably drought stress tolerance, of a plant by transforming the gene into a plant.

Hereinafter, the present invention will be described in detail by way of examples. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

Example  1: rice OsCTR1  Analysis of gene expression by organ and developmental stage

In order to confirm the expression pattern of OsCTR1 gene according to the organ and developmental stage of the rice plant (breed: during rice), the roots, stem and leaf organ of the early and late nutritive stage were collected, And spike were collected and RT-PCR was performed. The OsCTR1 gene-specific primer was prepared using Primer-BLAST (NCBI, http://www.ncbi.nlm.nih.gov/tools/primer-blast/) and the forward primer 5'-ACATCTCCAAGATGCTGTGC-3 ' No. 2), reverse primer 5'-AGCATGGCAGAGAACCAGAT-3 '(SEQ ID NO: 3) was used. As a result, the expression of OsCTR1 gene was not observed in the root of rice, and the expression of OsCTR1 gene was confirmed in postnatal growth stage in the stem, and in the postnatal growth stage and reproductive growth stage in the leaves . The expression of OsCTR1 gene was increased as the development of conidia progressed, and the expression of OsCTR1 gene was also observed in further differentiated aquatic plants. In order to verify that the same amount of RNA was compared in the electrophoresis experiment, the amount of Os18S - rRNA was observed for each lane. The primer used was a forward primer 5'-ATGATAACTCGACGGATCGC-3 '(SEQ ID NO: 4) and a reverse primer 5'- CTTGGATGTGGTAGCCGTTT-3 '(SEQ ID NO: 5) (FIG. 1A).

Example  2: by various environmental stress treatment OsCTR1 Characterization of gene expression

OsCTR1 In order to analyze the extent to which gene expression responds to various environmental stress treatments, plants were exposed to the following abiotic and biological stresses: dehydration, heat (45 ° C), low temperature (4 ° C), 0.1 mM ABA, 1 mM salicylic acid and 0.1 mM jasmonic acid treatment Respectively. Each stress treatment was applied to 2-week-old rice seedlings (seeds: rice) during seed germination, leaves were collected at 0, 1, 6, 12, 24 and 48 hours after treatment and then used for RT-PCR Respectively. OsbZIP23, OsHsp90-1 , OsLIP19 , OsSalT , OsPR1b and OsPBZ1 genes were used as a positive control for dehydration, heat, low temperature, ABA, salicylic acid and jasmonic acid treatment. Os18S- rRNA was compared with the same amount of RNA in the electrophoresis experiment The amount of each lane was observed. The primers used for gene amplification were OsbZIP23 forward primer 5'-CTCTGATCCCTCGTTGCGTTA-3 '(SEQ ID NO: 6) and Reverse primer 5'-CAACACCCCAGCACCAAACT-3 '(SEQ ID NO: 7), OsHsp90 -1 forward primer 5'-CTACGTGCGCCGCGTCTTCA-3' (SEQ ID NO: 8) and reverse primer 5'-CGGCTCGAACGACCTCTTCCTCT-3 '(SEQ ID NO: 9), OsLIP19 (SEQ ID NO: 10), reverse primer 5'-AGAAGGAGTGGGAACGGCGG-3 '(SEQ ID NO: 11), OsSalT (SEQ ID NO: 12) and reverse primer 5'-ACTGGGCCATGGGTTCCAGA-3 '(SEQ ID NO: 13), OsPR1b The forward primer 5'-CCTGGGACACGAGCGTGCAG -'3 (SEQ ID NO: 14) and the reverse primer 5'-TCGCTCTTCTCGCCCACCCA -'3 (SEQ ID NO: 15), OsPBZ1 The forward primer 5'-TGAGGTCGAGGGGGATGGCG-3 (SEQ ID NO: 16) and the reverse primer 5'-CGGCGGCCTCGATCTTCGTC-3 '(SEQ ID NO: 17).

As a result, expression of positive control gene was confirmed in heat or cold stress treatment during abiotic stress, but expression of OsCTR1 gene was not confirmed, and it was confirmed that expression of OsCTR1 gene was induced in dehydration stress treatment only from 12 hours to 48 hours there was. In addition, OsCTR1 gene expression was induced in the leaves of rice treated with ABA, salicylic acid and jasmonic acid (FIG. 1B).

Example  3: OsCTR1  Arabidopsis transfection experiments overexpressing genes

For the production of transgenic Arabidopsis thaliana overexpressing OsCTR1 gene, and fused genes OsCTR1 the CaMV35S promoter to drive expression of a gene at all times, the DNA construct Agrobacterium tumeo Pacific Enschede GV3101 (Agrobacterium tumefaciens GV3101) and induced Arabidopsis plant transformation according to floral dip transformation. The seeds obtained from these plants were germinated in kanamycin-containing medium to select kanamycin-resistant plants and tested for third generation seeds. RT-PCR analysis was performed to identify the target gene inserted in these transgenic Arabidopsis thaliana. The gene to be isolated from rice original does not exist in the Arabidopsis thaliana non-transformant (WT, 35S: EYFP) has not been detected in, transformants: The (35S OsCTR1-EYFP # 1, 4, 5) OsCTR1 Gene was identified (Figure 2).

Example  4: OsCTR1  Phenotyping of transgenic Arabidopsis thaliana with changes in gene expression

The expression of OsCTR1 in rice was induced by ABA treatment, suggesting that OsCTR1 is a gene whose ABA-dependent action is activated by ABA. To determine how the overexpressed OsCTR1 gene responds to environmental stress-related plant hormone ABA, germination rates were observed on MS medium containing 0.5 and 1 μM ABA. In the media containing no ABA, there was no difference in germination and growth of the transgenic and OsCTR1 transgenic overexpressants. However, in medium containing 0.5 and 1 μM ABA, the overexpressor of OsCTR1 gene was hypersensitized to ABA (Fig. 3).

Overexpression of OsCTR1 gene was observed in MS medium containing 0.5 and 1 μM ABA. In the media containing no ABA, there was no difference in the rate of cotyledon overgrowth of the non-transformant and OsCTR1 gene transcript. However, in the medium containing 0.5 and 1 μM ABA, OsCTR1 gene overexpressor was hypersensitized to ABA (Fig. 4).

The pore opening size of overexpressed Arabidopsis leaf of OsCTR1 gene was observed. In the medium containing no ABA, there was no difference in the mean pore opening size of the non-transformant and the OsCTR1 gene overexpressing medium, but in the medium containing 0.5 and 1 μM ABA, the OsCTR1 gene overexpressing substance was hypersensitized to ABA (Fig. 5).

Example  5: OsCTR1  Degeneration stress resistance experiment of transgenic Arabidopsis thaliana

First, the water loss of the OsCTR1 gene overexpressed Arabidopsis leaf was compared with that of the untranslated Arabidopsis leaf. Rosa leaves of two - week - old plants were removed and naturally dried, and weight loss was measured every 30 minutes for up to 6 hours. As a result, OsCTR1 gene overexpressed Arabidopsis leaves showed less water loss than non-transformed Arabidopsis leaves (Fig. 6A), and the shape of the removed leaves was longer than that of untransformed leaves (Fig. 6B).

The degree of accumulation of hydrogen peroxide in the overexpressed Arabidopsis thaliana leaf, OsCTR1 gene, which showed higher dehydration stress resistance than the control, was measured by histochemical staining. The transformed and untransformed Arabidopsis leaves at 90 and 180 minutes after dehydration treatment were immersed in a solution of 3, 3'-diaminobenzidine at a concentration of 1 mg / ml- ¹ for 4 hours, Ethanol: acetic acid: glycerol 3: 1: 1), boiled at 95 ° C for 15 minutes, and then stained. As a result, the accumulation of hydrogen peroxide was confirmed as the dehydration progressed in the transgenic Arabidopsis leaves (FIG. 7).

Example  6: OsCTR1  Drought tolerance test of gene transgenic Arabidopsis thaliana

OsCTR1 Drought tolerance experiments were conducted to determine whether the overexpressed Arabidopsis thaliana was resistant to drought stress. As a result of drought stress condition by not watering the transgenic Arabidopsis thaliana grown in the greenhouse for 2 weeks and non-transgenic Arabidopsis thaliana for 10 days, the OsCTR1 transgenic Arabidopsis thalamus was more resistant to drought stress than the untranslated Arabidopsis thaliana . After 3 days of recovery, the control group, the non-transgenic Arabidopsis thaliana, showed a survival rate of 18%, while the OsCTR1 transgenic transgenic Arabidopsis thalamus showed a high survival rate of 74% to 95% (Fig. 8).

<110> KNU-Industry Cooperation Foundation <120> OsCTR1 gene from rice for increasing drought stress resistance of          plant and uses thereof <130> PN13085 <160> 17 <170> Kopatentin 2.0 <210> 1 <211> 348 <212> DNA <213> Oryza sativa <400> 1 atgagctacc tcctctccta catctccaag atgctgtgca tcaagattcc gtcggaggcg 60 aggcaagcca gcgaggacgg cggcagcggt ggcctgacgg agtgctccgt ctgtctctcc 120 aggatccgtg tcggcgaggc gacgaggcgt ctgccctgcc ggcatgcgtt ccaccgggac 180 tgcgtcgacc ggtggctcct ctcatgcagg cggacgtgcc cgctttgccg ggtttacgtc 240 gtcgtcgacg gtaacaagcc aggggtggca gcgaagcaca ccggcgagcc gccgctcgct 300 gaagacatgg tgatctggtt ctctgccatg ctcgtccctg gattctga 348 <210> 2 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 2 acatctccaa gatgctgtgc 20 <210> 3 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 3 agcatggcag agaaccagat 20 <210> 4 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 4 atgataactc gacggatcgc 20 <210> 5 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 5 cttggatgtg gtagccgttt 20 <210> 6 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 6 ctctgatccc tcgttgcgtt a 21 <210> 7 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 7 caacacccca gcaccaaact 20 <210> 8 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 8 ctacgtgcgc cgcgtcttca 20 <210> 9 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 9 cggctcgaac gacctcttcc tct 23 <210> 10 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 10 agcccctcgg gttggtcgaa 20 <210> 11 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 11 agaaggagtg ggaacggcgg 20 <210> 12 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 12 ggtccgtggg gcggaaatgg 20 <210> 13 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 13 actgggccat gggttccaga 20 <210> 14 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 14 cctgggacac gagcgtgcag 20 <210> 15 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 15 tcgctcttct cgcccaccca 20 <210> 16 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 16 tgaggtcgag ggggatggcg 20 <210> 17 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 17 cggcggcctc gatcttcgtc 20

Claims (13)

A method for suppressing drought stress in plants comprising the step of over-expressing the OsCTR1 gene by transforming plant cells with a recombinant vector comprising a nucleotide sequence of SEQ ID NO: 1 and comprising a rice-derived OsCTR1 gene ( Oryza sativa chloroplast proteins targeting RING E3 ligase 1) Methods of increasing tolerance. delete delete delete Transposing a plant cell with a recombinant vector comprising a nucleotide sequence of SEQ ID NO: 1 and containing OsCTR1 ( Oryza sativa chloroplast proteins targeting RING E3 ligase 1) gene, and overexpressing the OsCTR1 gene; And
And regenerating the plant from the transgenic plant cell. &Lt; RTI ID = 0.0 &gt; 21. &lt; / RTI &gt; delete delete delete A transgenic plant having increased tolerance to drought stress produced by the method of claim 5. delete 10. The transgenic plant according to claim 9, wherein the plant is a dicotyledonous plant. 9. A plant seed according to claim 9. A composition for increasing the drought stress tolerance of a plant, which comprises as an active ingredient a recombinant vector comprising a rice-derived OsCTR1 gene ( Oryza sativa chloroplast proteins targeting RING E3 ligase 1) comprising the nucleotide sequence of SEQ ID NO: 1.

Images