KR101415706B1 - As Artemisia annua L and Orostachys japonicus A. Berger extract and its application to a method of manufacturing and health food - Google Patents
As Artemisia annua L and Orostachys japonicus A. Berger extract and its application to a method of manufacturing and health food Download PDFInfo
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- KR101415706B1 KR101415706B1 KR1020130121404A KR20130121404A KR101415706B1 KR 101415706 B1 KR101415706 B1 KR 101415706B1 KR 1020130121404 A KR1020130121404 A KR 1020130121404A KR 20130121404 A KR20130121404 A KR 20130121404A KR 101415706 B1 KR101415706 B1 KR 101415706B1
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- mugwort
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- 240000000011 Artemisia annua Species 0.000 title abstract 4
- 238000004519 manufacturing process Methods 0.000 title description 12
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- BMRSEYFENKXDIS-KLZCAUPSSA-N cis-3-O-p-coumaroylquinic acid Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)cc2)[C@@H]1O)C(=O)O BMRSEYFENKXDIS-KLZCAUPSSA-N 0.000 description 1
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Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/42—Preservation of non-alcoholic beverages
- A23L2/46—Preservation of non-alcoholic beverages by heating
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/20—Agglomerating; Granulating; Tabletting
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/14—Extraction
Abstract
Description
본 발명은 개똥쑥 및 와송(바위솔, 이하 와송이라 함)을 이용한 추출물과 이를 응용한 건강식품의 제조방법에 관한 것으로, 보다 상세하게는 개똥쑥과 와송에 함유된 각종 기능성을 액상의 음료와 건강식품으로 간편하게 섭취할 수 있도록 하는 개똥쑥과 와송을 이용한 음료와 이를 이용한 건강식품 및 그 제조방법에 관한 것이다.More particularly, the present invention relates to a method for producing various kinds of functional foods contained in white mugwort and green tea, such as liquid beverages and health foods, The present invention relates to a beverage, a health food using the same, and a method for producing the beverage.
일반적으로 쑥은 국화과(Compositae)의 쑥속(Artemisia)에 속하는 번식력이 강한 다년생 초본으로 한국이나 중국, 일본 등 아시아지역과 유럽지역 등에서 널리 분포되어 있으며, 국내에서는 약 300여 종이 자생하는 것으로 알려져 있다. Generally, mugwort is a perennial herbaceous herbaceous plant belonging to Artemisia of Compositae. It is widely distributed in Korea, China, Japan and other Asian countries and Europe. It is known that about 300 species are native to Korea.
쑥의 잎과 열매 등은 식용 및 약용으로 이용되어 왔으며 민간요법에서는 쑥의 전초를 말려 진정, 경련, 마비 및 전신 강직 등의 치료와 만성간염, 만성 위장염, 복통, 토혈, 식욕부진 등의 한방약재로 사용하여 왔다.Leaf and fruit of mugwort have been used for edible and medicinal purposes. In folk remedies, mugwort outpost is used to treat calm, convulsions, paralysis and systemic stiffness, and herbal medicines such as chronic hepatitis, chronic gastroenteritis, abdominal pain, .
현재 국내에서 인진쑥, 약쑥, 참쑥, 산쑥, 개똥쑥 등을 소재로 하여 다양한 연구가 이루어지고 있는데, 이 중 개똥쑥은 최근 클로로겐산(chlorogenic acid), 살리실산(salicylic acid) 등의 페놀산(phenolicacid)와 에피카테친(epicatechin), 카테친(catechin) 및 갈로-카테친 갈레이트(gallo-catechin gallate) 등의 카테친(catechin)류와 같은 성분들의 높은 항산화활성이 논문이나 문헌 등에 입증됨으로써 세계적으로 주목받고 있는 생약제로 평가되고 있다.Currently, various studies have been conducted in Korea on artificial wormwood, wormwood, japonica, wormwood, and dogwood wormwood. Of these, wormwood has recently been treated with phenolicacid such as chlorogenic acid, salicylic acid, The high antioxidant activity of components such as epicatechin, catechin and gallo-catechin gallate, such as catechin, has been proved in the literature and the literature, Is being evaluated.
또한, 상기 개똥쑥에 함유된 세스키테르펜(sesquiterpene)계의 아테미시닌(artemisinin)은 강력한 항말라리아 작용을 지닌 것으로 알려져 현재 의약품으로도 이용되고 있으며, 정유성분으로는 리날룰(linalool), 1,8-시네롤(1,8-cinerol), 로우-시멘(ρ-cymene), 투우존(thujone), 캄포(camphor) 등이 규명되어 있다.In addition, artemisinin, a sesquiterpene-based compound contained in the above-mentioned dog mugwort, is known to have a strong anti-malarial action and is currently used as a pharmaceutical product. Examples of essential oils include linalool, 1 , 8-cinerol, p-cymene, thujone, camphor, and the like have been identified.
이러한 개똥쑥에 대하여 현재까지의 논문이나 특허에서 일반음료로서의 제품에 대한 연구나 발명은 거의 없는 실정이다.
There have been few studies or inventions on the product as general beverage in the papers or patents to date.
와송(瓦松)은 바위솔(Orostachys japonicus A. Berger)이라 하며 일명 암송, 작엽하초, 옥송 등으로 불리는 돌나물과(Crassulaceae)의 다년생 초본 식물로서 한방에서 해열, 소종, 지혈, 이습 등에 사용되며, 민간요법으로 암치료에도 많이 이용되고 있다.It is a perennial herbaceous plant of Crassulaceae called Orostachys japonicus A. Berger. It is also used as a herbaceous herbaceous plant, herbaceous, hemostatic, and humidified in Oriental medicine. It is widely used for cancer treatment by therapy.
이 식물은 바위 곁에 붙어서 자라는 육질의 다년생 초본이지만 꽃이 피고 열매를 맺으면 고사하는 특징이 있다. 와송은 여름부터 가을에 걸쳐 채취하며 뿌리를 제거한 전초를 햇볕에 말려 약용으로 한다.This plant is a fruity perennial herb that grows attached to the rocks, but has the characteristic of stopping when flowers bloom and bear fruit. Wassong is taken from summer to autumn, and dried out in the sun to remove medicinal herbs.
와송을 본초학적으로 문헌을 통하여 고찰하여 보면 당본초(唐本草)에는 입안의 건조와 통증에 사용한다 고하며 본초강목에는 대장하혈에 물로 추출하여 복용하고, 모든 상처에 도포하면 수렴 효과가 있다고 하며, 본초재신에는 백독을 치료하고 종을 소실시키며 분류초약성에는 치질의 종통출혈을 다스리는 데 효과가 있다고 한다. 그리고, 의종금강 상, 하 8권에는 일찍이 악성 종양인 암치료에 와송이 주약으로 쓰였던 사실도 기록되어 있다. 이와같이 옛사람들은 와송의 효력을 믿고 비상약으로 썼으며, 와송의 뿌리를 잘라내고 잎이나 꽃을 찧어서 벌레 독사에게 물린 상처나 악성종기 등에 발라 해독약으로도 썼다.It is said that it is used for dryness and pain of the mouth in Tang Hwangcho and it is said that it is used for extracting water from cholangiocarcinoma with water and applying it to all wounds, , The treatment of blackheads and loss of species in the primary rehabilitation of the classification of the superficial hemorrhage of hemorrhoids is effective in treating hemorrhoids. In addition, there are records of the fact that Won Song was used as a medicine for the treatment of malignant tumors, which was an early malignant tumor. In this way, the old people believed in the efficacy of warsong, wrote it as emergency medicine, cut off the roots of warships, put leaves and flowers on it, and used it as a detoxifying medicine by putting on scars and malignant boils bites by insect serpents.
와송의 경우 공개특허 10-2011-0042442호에서 여러 종의 약제와 혼합된 음료는 있으나, 개똥쑥과 와송을 혼합한 음료는 없었다.In the case of < RTI ID = 0.0 > WASONG, < / RTI > the disclosure of Japanese Patent Application Laid-Open No. 10-2011-0042442 discloses a drink mixed with various kinds of medicines.
이에 본 발명에서는, 개똥쑥과 와송에 함유된 기능성 성분을 액상의 음료로 제조함에 있어 기능성 성분의 파괴가 최소화될 수 있도록 하면서 상승효과를 얻을 수 있도록 하고, 간편하게 휴대할 수 있도록 함과 동시에 때와 장소에 구애 없이 간편하게 복용할 수 있도록 함으로써 상품성과 경제성을 향상시킬 수 있도록 하는 개똥쑥과 와송을 이용한 추출물과 이를 응용한 건강식품의 제조방법을 제공함에 그 목적이 있다.Accordingly, in the present invention, in manufacturing a functional ingredient contained in milk powder and wort, it is possible to obtain a synergistic effect while minimizing the breakdown of the functional ingredient, and to carry it easily and at the same time, The present invention is directed to a method for manufacturing a health food using the same, and a method for manufacturing a health food using the same.
상기와 같은 목적을 달성하기 위한 본 발명의 개똥쑥과 와송을 이용한 추출물 제조방법은, 총중량에 대하여 건조한 개똥쑥 0.1-2중량%를 첨가하여, 가열온도 70-80℃에서 4시간-8시간 열수추출하고, 총중량에 대하여 건조한 와송0.01-1중량%를 첨가하여 가열 온도 115 ~ 120℃에서 70 ~ 90분 동안 열수추출하며, 각각 열수추출 후 고형물을 제거하고 추출액을 얻는 열수추출 단계와, 상기 개똥쑥과 와송 열수 추출액을 혼합하는 단계를 포함하는 개똥쑥 및 와송 추출액을 이용한 음료 제조 방법에 있다.In order to achieve the above object, the present invention provides a method for preparing an extract using caterpillars and salting, which comprises adding 0.1-2% by weight of dried caterpillar fungus to the total weight and heating at a heating temperature of 70-80 ° C for 4 hours to 8 hours. And extracting hot water for a period of 70 to 90 minutes at a heating temperature of 115 to 120 ° C to remove the solid matter after hot water extraction to obtain an extract, Which comprises a step of mixing mugwort and hot spring water extract, and a method of manufacturing beverage using the extract of mugwort and Wassong.
그리고, 상기 개똥쑥과 와송 열수 추출액의 혼합액을 농축기에 투입하여 농축하되 농축기에 투입된 전체 중량의 10%가 되도록 농축하는 농축단계와, 상기 농축단계에서 얻어진 농축액 100중량부에 대하여 10중량부에 해당하는 쌀 전분을 첨가 및 혼합하여 환, 정, 캡슐 또는 과립형태의 고형물로 제조하는 성형단계를 포함하여 구성되는 건강식품 제조 방법에 있다.Then, the mixture is concentrated to a concentration of 10% of the total weight added to the concentrator by adding the mixture of the above-mentioned methanol solution and hot water extract to the concentrator, and 10 parts by weight Wherein the rice starch is added to and mixed with rice starch to form a solid, such as a ring, tablet, capsule or granule.
또한, 본 발명의 다른 특징은 상기한 제조방법으로 제조된 음료 및 건강식품에 있다.In addition, another feature of the present invention resides in beverages and health foods produced by the above-described production methods.
상기한 바와 같이 구성된 본 발명 의하면 개똥쑥과 와송에 함유된 기능성 성분을 간편하게 휴대할 수 있도록 함과 동시에 때와 장소에 구애 없이 복용할 수 있기 때문에, 상품성과 경제성을 향상시킬 수 있는 효과가 있다.According to the present invention having the above-described constitution, the functional ingredient contained in the artillery wormwood and the wort can be easily carried, and at the same time, the product can be taken without adhering to the time and place.
도 1은 본 발명의 실시 예에 따른 제조공정도1 is a schematic view showing a manufacturing process according to an embodiment of the present invention
이하 본 발명의 실시예를 하기에서 첨부된 도면을 참조하여 설명한다.
Hereinafter, embodiments of the present invention will be described with reference to the accompanying drawings.
도 1은 본 발명의 실시 예에 따른 제조공정을 도시한 도면으로써, 개똥쑥과 와송 추출물을 이용한 음료 및 건강식품 제조방법을 나타낸다.
FIG. 1 is a view showing a manufacturing process according to an embodiment of the present invention, and shows a method of manufacturing a beverage and a health food using a mugwort and a Wassong extract.
먼저, 개똥쑥은 재배된 전초를 채취한다 . 와송은 재배된 것을 구입하여 각각 흐르는 물로 세척을 하고, 물기를 제거한 후, 건조한다.First, it collects the planted outpox. Purchase the cultivated wafers, wash them with flowing water, remove the water, and dry them.
건조된 개똥쑥과 와송을 적당한 크기로 세절하고, 개똥쑥 0.1-2중량%와 와송0.01-1%를 각각 정제수 98 내지 99.9 중량% 및 99 내지 99.99중량%와 함께 추출기에 넣고, 개똥쑥의 경우 가열온도 70-80℃에서 4시간-8시간, 와송 가열 온도 115℃ 내지 120℃에서 70 내지 90분 동안 가열한다.Dried mugwort and mugwort were sieved to an appropriate size, and 0.1-2% by weight of mugwort and 0.01-1% of wort were added to an extractor together with 98 to 99.9% by weight and 99 to 99.99% by weight of purified water, respectively, Heating at a heating temperature of 70-80 占 폚 for 4 hours to 8 hours, and a heating temperature of 115 占 폚 to 120 占 폚 for 70 to 90 minutes.
상기 가열이 완료되면, 추출기의 내부에 있는 개똥쑥과 와송의 입자상의 각종 이물질을 제거하여, 개똥쑥과 바위솔 추출액을 얻는다.When the heating is completed, various kinds of foreign substances in the inside of the extractor are removed to obtain an extract of Mt.
이와 같이 얻어진 개똥쑥과 와송 추출액을 혼합한 후, 한번 더 여과망을 통과시켜 미립자형태의 이물질까지 제거한다.After mixing the thus-obtained white mugwort and fresh water extract, the fine mugwort-like extract is further passed through the filter net to remove foreign matter in the form of fine particles.
상기와 같이 혼합된 개똥쑥과 와송 추출액의 혼합액을 90℃를 유지한 상태에서 파우치 팩에 100-200mL주입하여 자동으로 밀봉, 포장한다. 이때 온도를 90℃로 유지해 주어야 적어도 1년까지 보관이 용이하다.100-200 mL of the mixture of the above-mentioned mixture of mugwort-mugwort and fresh mugwort extract is poured into the pouch pack while maintaining the temperature at 90 ° C and sealed and packed automatically. The temperature should be maintained at 90 ° C for at least 1 year.
상기와 같은 제조과정에 의해 제조되는 액상의 추출물은 100~200mL 단위의 포장 팩에 진공포장되기 때문에 장시간 동안 보관이 용이할 뿐만 아니라, 간편하게 휴대가 가능하여 장소에 구애 없이 정해진 시간 안에서 언제든 음용 할 수 있다.
Since the liquid extract prepared by the above-mentioned manufacturing process is vacuum-packed in a packing pack of 100 to 200 mL unit, it is easy to store for a long time and can be easily carried, so that it can be consumed at any time within a predetermined time have.
삭제delete
그리고 개똥쑥과 와송 추출액을 이용한 건강식품의 제조방법은, 상기 개똥쑥과 바위솔 추출액을 전용 농축기에 투입하여 농축하되 농축기에 투입된 전체 중량의 10%가 되도록 농축한 다음, 상기 농축단계에서 얻어진 농축액에 쌀 전분을 첨가하되, 농축액 100중량부의 10중량부를 첨가하여 혼합하고, 건조한 후, 환, 정, 캡슐, 과립 중 어느 하나의 건강식품으로 성형함과 동시에 상기 건강식품성형단계에서 성형 된 건강식품을 자동으로 밀봉 포장하는 포장단계로 이루어진다.The method for preparing a health food using the mugwort and the salted mugwort extract according to the present invention is characterized in that the mugwort and the gossypol extract are added to a concentrator and concentrated to 10% of the total weight of the concentrate, 10 parts by weight of rice starch is added and 10 parts by weight of 100 parts by weight of the concentrate is added and mixed, and the mixture is dried and molded into a health food such as a ring, a pill, a capsule or a granule, And a packaging step of automatically sealing and packaging.
상기 건강식품에서 환, 정, 캡슐, 과립 등을 성형할 때는 공지의 전용 제환기, 제정기, 캡슐기, 과립기에서 성형하면 되며, 포장과정 역시 해당 식품에 알맞게 개별 또는 PET병, 유리병 등에 포장할 수 있다.
When molding rings, tablets, capsules, granules and the like in the above-mentioned health food, it may be molded in a well-known dedicated ventilator, a tablet machine, a capsule machine and a granulator. Can be packed.
실시예Example 1 One
정제수 9800g과 개똥쑥 200g을 추출기에 투입한 다음 70-90℃에서 4-8시간 가열하고, 정제수 9900g과 와송 100g을 추출기에 투입한 다음 가열온도 115 - 120℃에서 70 내지 90분 동안 가열한 후 고형물을 제거하여 추출액을 얻는다.9800 g of purified water and 200 g of mugwort were added to the extractor, heated at 70-90 ° C for 4-8 hours, 9900 g of purified water and 100 g of the supernatant were added to the extractor, heated at a temperature of 115-120 ° C. for 70-90 minutes The solids are removed to obtain an extract.
이 과정에서 정제수는 중량기준 약10% 감소 되었으며, 미색의 개똥쑥과 와송 추출액을 얻을 수 있으며, 그 추출액을 전용 농축기에서 전체 중량의 10%가 되게 농축한 다음, 상기 농축액 100중량부에 대하여 10중량부에 해당하는 쌀 전분을 첨가 및 혼합하여 환, 정, 캡슐 또는 과립형태의 고형물로 건강식품을 제조한다.
In this process, the purified water was reduced by about 10% by weight, and the off-white powdery mugwort and the dried persimmon extract were obtained. The extract was concentrated to 10% of the total weight in a dedicated concentrator, The rice starch corresponding to the weight part is added and mixed to prepare a health food as a solid in the form of a ring, tablet, capsule or granule.
실험 결과Experiment result
1. ABTs 라디칼 소거1. ABTs radical scavenging
(1) 실험방법(1) Experimental method
ABTs 라디칼 소거능을 알아보기 위해 Re R 등(1999)의 방법에 따라 7mM ABTs 용액에 과황산칼륨(potassium persulfate)를 2.4mM이 되도록 용해시킨 다음 암실에서 12~16시간 동안 반응시켰다. 이를 414nm에서 흡광도가 1.5가 되도록 증류수로 희석하였다. 이 용액 3mL에 10, 25, 50, 100ppm의 농도로 조제한 각 시료 1mL를 가하여 실온에서 10분간 반응시켜 414nm에서 흡광도를 측정하였으며, ABTs 라디칼 소거능은 시료 첨가구와 무첨가구의 흡광도 비로 나타내었다.To investigate the radical scavenging ability of ABTs, potassium persulfate was dissolved in 7 mM ABTs solution to 2.4 mM according to the method of Re R et al. (1999) and reacted for 12-16 hours in a dark room. This was diluted with distilled water to an absorbance of 1.5 at 414 nm. 1 mL of each sample prepared at a concentration of 10, 25, 50, and 100 ppm was added to 3 mL of this solution, and reacted at room temperature for 10 minutes to measure the absorbance at 414 nm. The ABTs radical scavenging activity was represented by the absorbance ratio of the sample without and with no addition.
(2)실험결과(2) Experimental results
와송 추출물과 개똥쑥 추출물의 ABTs 라디칼 소거활성은 [표 1]에 나타낸 바와 같다. 각 추출물의 농도가 증가할수록 ABTs 라디칼 소거활성 또한 증가하지만 와송 추출물에 비해 개똥쑥 추출물의 ABTs 라디칼 소거활성이 더 높았다.그러나 와송 추출물의 농도가 높을수록 개똥쑥 추출물에비해 ABTs 라디칼 소거활성의 차이는 없었다The ABTs radical scavenging activities of Wassong extract and Lauroginae japonica extract were as shown in Table 1. The ABTs radical scavenging activity was higher in the extracts of A. japonicus than in the Wassong extract, but the ABTs radical scavenging activity of the extracts was higher than that of the A. japonica extract No
와송 추출물과 개똥쑥 추출물의 혼합물은 추출물 각각을 0.5mL씩 첨가하여 1mL로한 것으로, 와송 추출물이나 개똥쑥 추출물의 단독보다 혼합한 것이 ABTs 라디칼 소거활성이 최소 1.5배에서 최대 7.3배까지 향상됨을 알 수 있다.
It was found that the mixture of Wassong extract and Laurogusa japonica extract was prepared by adding 0.5 mL of each of the extracts to 1 mL, and that the mixture of Wassong extract and Lauroginae extract alone increased the ABTs radical scavenging activity from at least 1.5 times to at most 7.3 times have.
2. DPPH 라디칼 소거활성2. DPPH radical scavenging activity
(1)실험방법(1) Experimental method
1,1-Diphenyl-2-picrylhydrazyl(DPPH) 라디칼에 대한 소거효과는 Blios MS(1958) 방법에 준하여 평가하였다.The scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals was evaluated according to the Blios MS (1958) method.
10, 25, 50, 100ppm 농도의 추출물 2mL에 DPPH 라디칼 2mL를 가하였다. 혼합물을 교반하여 암소에서 30분간 방치한 다음 517nm에서 흡광도를 측정하여 DPPH 라디칼 소거활성을 시료 첨가구와 무첨가구의 흡광도 비로 나타내었다.2 mL of the DPPH radical was added to 2 mL of the extract at concentrations of 10, 25, 50 and 100 ppm. The mixture was allowed to stand in the dark for 30 minutes with stirring, and the absorbance at 517 nm was measured. The DPPH radical scavenging activity was expressed as the absorbance ratio of the sample without and with no addition.
(2)실험결과(2) Experimental results
개똥쑥 추출물과 와송의 추출물의 DPPH 라디칼 소거활성은 [표 2]에 나타낸 바와 같다. 각 추출물의 농도가 증가할수록 DPPH 라디칼 소거활성 또한 증가하지만 와송(%)에 비해 개똥쑥 추출물의 DPPH 라디칼 소거활성이 더 높았다.The DPPH radical scavenging activity of the extracts of Mugwort mugwort and Wassong was as shown in Table 2. DPPH radical scavenging activity was also increased with increasing concentration of each extract, but the DPPH radical scavenging activity was higher in the extract of Cryptomeria japonica than the percentage of free radicals (%).
그러나 와송 + 개똥쑥추출물의 경우는 와송 추출물이나 개똥쑥 추출물의 단독보다 혼합한 것이 DPPH 라디칼 소거활성이 최소 1.15배에서 최대 6.7배 더 높았다.
However, in the case of the extract of Wassong + Cryptomeria japonica, the mixture of the extracts of Wassong and Cryptomeria japonica showed a DPPH radical scavenging activity of at least 1.15 times and a maximum of 6.7 times higher.
3. 와송 추출액과 개똥쑥 추출액의 혼합물에 대한 항산화 활성3. Antioxidative activity against a mixture of Wassong extract and Lauroginae extract
[표 3]에서와 같이 개똥쑥 추출액과 와송 추출액을 99:01(A), 95:5(B), 90:10(C)의 중량비로 혼합한 시료의 DPPH와 ABTs라디칼 소거 활성은 개똥쑥 추출액의 혼합비가 많아질수록 증가하였다. 나아가 개똥쑥 추출액 단독의 항산화 활성에 비해 항산화 활성이 월등히 높았던 와송 추출액이 첨가됨으로써 혼합물의 항산화 활성이 더욱 증가함을 확인할 수 있었다.
The DPPH and ABTs radical scavenging activities of the samples prepared by mixing the rape extract and the salted extract at the weight ratio of 99: 1 (A), 95: 5 (B) and 90:10 (C) And increased as the mixing ratio of the extract increased. Furthermore, the antioxidant activity of the mixture was further increased by the addition of the extract of Wansong, which had a much higher antioxidative activity than that of the extract alone.
4. 관능검사4. Sensory evaluation
본 발명의 개똥쑥과 와송 추출액의 첨가량에 따른 액상식품의 복용 용이성 확인을 위하여 관능검사를 실시하였다. 패널은 식품영양학과 4학년 학생 중 남자 25명, 여자 15명 대학원생 10명 총 50명을 선정하여 1회에 2가지의 시료를 제시하고 10회에 걸쳐 시료의 반복횟수는 3번으로 하여 시료의 외관, 냄새, 맛, 종합적 기호도를 다음과 같은 9점척도법에 의해 평가하도록 하였다. 결과는 SAS(Statistical Analysis System)를 이용하여 ANOVA test후 Duncan's multiple range test로 시료 간의 유의성은 5% 수준에서 최소 유의차 검정(leastsignificant difference test)으로 조사하였다. 이상의 관능 검사 결과는 표 4에 나타냈다.The sensory evaluation was carried out to confirm the ease of taking liquid food according to the amount of cocoon mugwort and fresh water extract of the present invention. In the panel, a total of 50 men, 25 male and 15 female graduate students, were selected from 4th grade students in Food and Nutrition Department, and two samples were presented at one time. The number of repetition of samples was 10 times, Appearance, smell, taste, and overall preference were evaluated by the following 9 point scale method. The results were analyzed by SAS (Statistical Analysis System), Duncan's multiple range test after ANOVA test, and the least significance test at 5% level. The results of the above sensory test are shown in Table 4.
실시예 1 : 개똥숙0.1% + 와송0.01% 혼합Example 1: Mixing with 0.1% of goat milk and 0.01% of water
실시예 2 : 개똥숙0.5% + 와송0.05% 혼합Example 2: Mixing with 0.5% of goat milk + 0.05%
실시예 3: 개똥숙1.0% + 와송0.1% 혼합
Example 3: Mixing with 1.0% of goat milk + 0.1%
그 결과, 실시 예1과 실시 예 2 및 실시 예 3 의해 제조된 액상식품의 경우 외관이나 냄새, 맛의 차이는 거의 비슷하며 종합적인 기호도의 경우는 실시 예 3(개똥숙1.0% + 와송0.1%) 혼합이 약간 높았다.As a result, the appearance, odor, and taste of the liquid food prepared according to Example 1, Example 2, and Example 3 were almost similar, and in the case of the general preference, the liquid food prepared according to Example 3 (1.0% ) Mixing was slightly higher.
Claims (4)
상기 개똥쑥과 와송 열수 추출액을 중량비로 99:01 ~ 90:10로 혼합하는 단계를 포함하는 것을 특징으로 하는 개똥쑥 및 와송 추출액을 이용한 음료 제조 방법.0.1 to 2% by weight of dried mugwort and 0.01 to 1% of dried mugwort were mixed with purified water of 98 to 99.9% by weight and 99 to 99.99% by weight, respectively, and the mugwort was heated at 70 to 80 ° C for 4 to 8 hours A hot water extraction step of hot water extraction at 115 ° C to 120 ° C for 70 minutes to 90 minutes, a hot water extraction step of removing solid matter after hot water extraction and obtaining an extract,
The method of claim 1, further comprising mixing the rabbit mugwort and the hot water extract of Takara Shuzo at a weight ratio of 99: 1 to 90:10.
상기 개똥쑥과 와송 열수 추출액을 중량비로 99:01 ~ 90:10로 혼합하는 단계,
상기 개똥쑥과 와송 열수 추출액의 혼합액을 농축기에 투입하여 농축하되 농축기에 투입된 전체 중량의 10%가 되도록 농축하는 농축단계와,
상기 농축단계에서 얻어진 농축액 100중량부에 대하여 10중량부에 해당하는 쌀 전분을 첨가 및 혼합하여 고형물로 제조하는 성형단계를 포함하여 구성되는 것을 특징으로 하는 개똥쑥 및 와송 추출액을 이용한 건강식품 제조 방법.0.1 to 2% by weight of dried mugwort and 0.01 to 1% of dried mugwort were mixed with purified water of 98 to 99.9% by weight and 99 to 99.99% by weight, respectively, and the mugwort was heated at 70 to 80 ° C for 4 to 8 hours A hot water extraction step of hot water extraction at 115 ° C to 120 ° C for 70 minutes to 90 minutes, a hot water extraction step of removing solid matter after hot water extraction and obtaining an extract,
Mixing the shag mugwort with the hot water extract of Tosun water at a weight ratio of 99: 1 to 90:10,
A concentrating step of concentrating the mixture solution of the mutton mugwort and the hot water extract solution into a concentrator and concentrating the concentrate to 10% of the total weight of the concentrator;
And a molding step of adding 10 parts by weight of rice starch to 100 parts by weight of the concentrated liquid obtained in the concentration step to prepare a solid material. .
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KR20160070338A (en) | 2014-12-10 | 2016-06-20 | 박창호 | Method for manufacturing health food including wasong |
KR20170014034A (en) | 2015-07-28 | 2017-02-08 | 재단법인 포항테크노파크 | Functional health foods and their preparation containing powdered Crassulaceae and cactus |
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KR101653585B1 (en) * | 2014-07-30 | 2016-09-07 | 김손란 | Method of manufacturing houseleek-sauce and seafood marinated in houseleek-sauce |
KR20160070338A (en) | 2014-12-10 | 2016-06-20 | 박창호 | Method for manufacturing health food including wasong |
KR20170014034A (en) | 2015-07-28 | 2017-02-08 | 재단법인 포항테크노파크 | Functional health foods and their preparation containing powdered Crassulaceae and cactus |
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