KR101374478B1 - Composition and kit for preparing a cell block, and method for preparing cell blocks using the same - Google Patents

Abstract

The present invention provides a composition for producing a cell block comprising a plurality of cells to be observed, a kit for producing a cell block comprising the same, and a method for producing a cell block using the same. According to the present invention, it is possible to provide a composition for preparing a cell block, which can minimize cell deformation during cell block production, and is capable of observing cells in various directions by transplanting and cutting cells stacked in multiple layers in a desired direction. .

Description

Compositions, kits for preparing cell blocks, and methods for preparing cell blocks using them {Composition and kit for preparing a cell block, and method for preparing cell blocks using the same}

The present invention relates to cell block preparation comprising one or more cells. More specifically, the present invention relates to a composition for preparing a cell block for producing a cell block from a cell line or cell suspension, a kit comprising the same, and a method for producing a cell block using the same.

The most basic task of the pathology task is to read the changes of cells, tissues, organs, etc. collected by biopsy or surgery by optical microscope to diagnose the disease and to identify the etiology, epidemiology and course of the disease. During cell readout, cells obtained through histological examination or the like are generally cultured to observe the change in appearance of the proliferated cells.

In the case of culturing cells in a culture dish, trypsin / phosphate buffer solution (PBS) is generally treated to desorb the cells attached to the bottom of the culture dish. This trypsin treatment causes the cell surface material to be degraded so that the cells are cultured. Desorption from However, reagents such as trypsin are toxic and can cause cell degeneration and death. As a cell block manufacturing method for observing detached cells, the desorbed cells are collected by centrifugation, fixed in formalin solution, and the fixed cells are collected and gelated using 1xPBS in which 1% agarose is dissolved. Methods of making cell blocks are well known (see http://www.ihcworld.com/_protocols/histology/cell_block.htm). At this time, in order to dissolve agarose, 1xPBS containing agarose is boiled and then cooled enough to prevent cell denaturation and mixed with cells before solidification to obtain a cell block. The temperature of the cells fixed according to the temperature of the 1xPBS solution in which the agarose is mixed with the cells may be increased, and then, as the temperature is lowered during cooling, relaxation of the cells may occur and deformation of the cell appearance may occur. That is, the 1xPBS solution in which 1% agarose is dissolved in the conventional cell block can control the osmotic pressure to prevent elution of water in the cell, but it may cause cell deformation due to temperature change, and thus, when cutting and observing the cell block. Often the desired sample form was difficult to obtain. In this case, cells were also randomly distributed in the cell block, which made it difficult to cut into desired cell forms.

Accordingly, the present inventors have attempted to develop a cell block manufacturing method for cell observation by stacking cells in a desired form while minimizing cell deformation.

The present invention is to provide a composition for producing a cell block that can be utilized in pathological processes such as histology and a method for producing a cell block using the same.

The present invention, if you want to observe the cells obtained by histological examination, etc., to provide a composition for producing a cell block and a kit comprising the same can increase the cell viability and minimize cell transformation.

The present invention is to provide a method for producing a cell block comprising cells stacked in a desired direction, such that the cutting is possible to observe the cells at a desired angle.

One aspect of the present invention relates to a composition for preparing a cell block for observing cells for various purposes, 0.5 to 2% by weight agarose, 5 to 20% by weight sugars, sodium chloride (NaCl) or a mixture thereof, And a residual amount of water and / or phosphate buffer solution (PBS).

Another aspect of the invention relates to a cell block preparation kit comprising a composition for cell block preparation according to the invention, a cell harvesting means, a cell block preparation frame, and optionally instructions for use.

Another aspect of the invention relates to a method for producing a cell block comprising cells from a cell line or cell suspension using the cell block preparation composition or cell block preparation kit described above.

According to an embodiment of the present invention, cells can be stacked in a cell block in a desired direction, and a cell block including cells in which cell morphology is maintained can be prepared by minimizing cell deformation, thereby cutting the cell block in a desired direction. Cell sections can be observed.

)와 상대적으로 높은 온도일 때(

)의 수분 활성도 및 수분 함유량의 관계를 나타낸 그래프이다. 도 1에서 가로축은 수분 활성도를, 세로축은 물체 중의 수분 함유량(수분 수용량)을 나타내며, (b)는 (a)에서 상대적으로 낮은 수분 활성도 부분을 확대하여 도시한 그래프이다.
도 2는 본 발명에 따른 세포 블록에서 온도의 변화 및 시간의 경과에 따른 수분 활성도 변화로 인하여 당류 또는 염화나트륨이 세포 주변에 얇은 막을 형성함으로써 결정화 구조가 얻어진 상태를 개략적으로 도시한 도면이다.
도 3은 본 발명에 따른 세포 블록의 모형도로서, 세포 블록이 냉각됨에 따라 도 2에 도시된 바와 같이 세포 주변에 당류나 염화나트륨의 결정성 막이 형성되면서 세포 블록 조성물 내의 아가로오즈에 의하여 경화되어, 세포 내 수분 등의 손실 없이 세포가 원형 그대로 유지되어 포함되어 있는 세포 블록의 형성 과정을 개략적으로 도시한 도면 및 세포 블록 완성 후의 세포 부분 확대도이다.
도 4는 기존의 세포 블록 제조용 용액(1% 아가로오즈가 용해된 1xPBS 용액)을 사용하여 제조된 세포 블록의 모형도로서, 온도 변화에 따라 세포의 변형이 일어난 상태를 개략적으로 도시한 도면이다.
도 5는 본 발명에 따른 세포 블록 제조용 조성물을 포함하는 용기의 일 예를 개략적으로 도시한 도면이다.
도 6은 본 발명에 따라 세포 채취 수단을 사용하는 방법의 일 예를 도시한 도면이다.
도 7은 본 발명에 따른 세포 블록 제조용 틀의 일 예를 도시한 도면이다.
도 8은 본 발명의 일 구현예에 따라 세포 블록 제조용 틀에서 성형된 세포 블록을 분리해내는 과정을 도시한 도면이다.
도 9는 본 발명의 일 구현예에 따라 배양 접시에서 배양된 세포를 채취하여 세포 블록을 제조하는 방법의 일 예를 도시한 도면이다.
도 10은 본 발명에 따라 얻어진 세포 블록을 절단하여 세포를 관찰하는 방법을 예시적으로 도시한 도면이다.
도 11은 본 발명의 한 구현예에 따라 세포 현탁액(계대 배양 세포)으로부터 세포를 분리하여 세포 블록을 제조하는 방법의 일 예의 도시한 도면이다. 1 is a view to help understand the implementation principle of the present invention, when the low temperature (

) And relatively high temperatures (

) Is a graph showing the relationship between water activity and water content. In Figure 1, the horizontal axis represents water activity, the vertical axis represents water content (water content) in the object, and (b) is a graph showing an enlarged portion of relatively low water activity in (a).
FIG. 2 is a view schematically showing a state in which a crystallized structure is obtained by forming a thin film around a cell due to a change in temperature and a change in water activity over time in a cell block according to the present invention.
FIG. 3 is a schematic diagram of a cell block according to the present invention. As the cell block is cooled, it is cured by agarose in the cell block composition while forming a crystalline membrane of saccharide or sodium chloride around the cell as shown in FIG. It is a schematic diagram illustrating the process of forming a cell block in which cells are kept intact without loss of intracellular moisture and the like, and an enlarged view of a cell part after completion of the cell block.
FIG. 4 is a schematic diagram of a cell block prepared using a conventional cell block preparation solution (1 × PBS solution in which 1% agarose is dissolved), and schematically illustrates a state in which cell deformation occurs according to temperature change.
5 is a view schematically showing an example of a container including a composition for producing a cell block according to the present invention.
6 is a view showing an example of a method using a cell harvesting means according to the present invention.
7 is a view showing an example of a cell block manufacturing frame according to the present invention.
8 is a view showing a process of separating the molded cell block in the cell block manufacturing frame according to an embodiment of the present invention.
9 is a view showing an example of a method for producing a cell block by collecting the cultured cells in a culture dish according to an embodiment of the present invention.
10 is a diagram illustrating a method of observing cells by cutting a cell block obtained according to the present invention.
11 is a diagram illustrating an example of a method of preparing a cell block by isolating cells from a cell suspension (passage cultured cells) in accordance with one embodiment of the present invention.

The first aspect of the present invention is a 0.5 to 2% by weight agarose, 5 to 20% by weight sugar, sodium chloride (NaCl) or a mixture thereof, and the balance of water and / or phosphate buffer solution (PBS) It provides a composition for producing a cell block comprising a). The method for preparing a cell block preparation gel from the cell block preparation composition according to the present invention can be easily carried out by a person skilled in the art based on techniques known in the art. For example, a predetermined amount of sugar, sodium chloride (NaCl) or a mixture thereof and agarose may be added to water, 1 × PBS, or a mixed solvent thereof, and they may be sufficiently dissolved at a temperature such that sugars and agarose can be sufficiently dissolved. It is heated for a time that can be dissolved and then cooled to an appropriate temperature, for example, 30 to 38 ° C.

According to one embodiment of the present invention, the composition for preparing a cell block in which agarose and saccharides are completely dissolved is added dropwise onto a cell line cultured on a culture dish, and then applied to the cell line surface, and then one or more cell lines are separated. Can be used to When the composition for cell block preparation according to the present invention is applied onto the cultured cell line as described above, cells can be easily separated from the culture dish without treatment of reagents such as trypsin, thereby minimizing cell denaturation and death by the reagents.

The composition for cell block preparation according to the present invention can also be used to prepare a gel block for cell block preparation for transplanting isolated cell lines as described above.

According to another embodiment of the present invention, a cell block preparation composition in which agarose and saccharides are completely dissolved is mixed with a cell suspension containing one or more cells, and then used to prepare a cell block in a gel state. Can be.

In the present invention, the "cell" is interpreted to include all animal cells, plant cells, and microbial cells without distinguishing between prokaryotic and eukaryotic cells.

As used herein, the term "saccharides" is not particularly limited as long as it can form a thin film around the cells when treated in a cell line or cell suspension to prevent cell deformation, for example, monosaccharides such as glucose, fructose, sucrose (sugar) ), Maltose, disaccharides such as lactose, polysaccharides such as cellulose, starch and glycogen. More specific examples of the sugars include monosaccharides such as glucose, fructose, sucrose, maltose, lactose and disaccharides, and commercially available refined sugar.

In the present invention, sodium chloride may be used for both reagent or pharmaceutical sodium chloride or commercial edible salt, preferably using purified salt.

In the present invention, the solvent used to dissolve the components such as agarose is water, 1xPBS, or a mixed solvent in which they are mixed in any ratio. Among these solvents, water, more preferably distilled water, and most preferably tertiary distilled water.

The second aspect of the present invention relates to a method for producing a cell block using the composition for producing a cell block according to the present invention.

According to one embodiment, the method for producing a cell block according to the present invention comprises the steps of: (a) applying a composition for producing a cell block according to the present invention on cultured cells, (b) collecting a cell with one or more cells to which the composition is applied. Collecting at least one time by means, and (c) injecting the collected cells into the cell block gel obtained by molding the cell block manufacturing composition.

More specifically, the cell block preparation method according to the present invention comprises 0.5 to 2% by weight of agarose and 5 to 20% by weight of saccharides, sodium chloride (NaCl) substantially completely dissolved in water and / or 1xPBS solvent. ) Or providing a composition for producing a cell block comprising a mixture thereof, applying the composition at 30 to 38 ℃ on the cultured cells, one or more cells using a cell harvesting means (e.g., a piston, etc.) Harvesting more than once, cells comprising from 0.5 to 2% by weight agarose and 5 to 20% by weight of saccharides, sodium chloride (NaCl) or mixtures thereof substantially completely dissolved in water and / or 1xPBS solvent It may include the step of injecting the cells collected in the cell block gel molded from the composition for block production. The cell block manufacturing method according to this process can be easily understood based on Preparation Example 1 below (see FIG. 7).

According to the present invention, a cell block in which one or more cells are stacked in one or more layers and collected in a cell harvesting means, injected into a cell block molded in a gel state, and then cooled and laminated with the cell block in which the gel block is integrated You can get it. The gel block at the time of cell injection is preferably in the gel state before being completely cured to facilitate cell injection, and more preferably, when the temperature of the cell block composition is at most 50 ° C, most preferably at 30 to 38 ° C. Inject the cells. Curing and storage after cell block production takes place at temperatures below 30 ° C. For long term storage of cell blocks, it is desirable to store them at a low temperature of 4 ° C. or lower.

Another aspect of the invention is a substantially complete dissolution of 0.5-2% by weight agarose and 5-20% by weight of saccharides, sodium chloride (NaCl) or mixtures thereof in a cell population comprising one or more cells. It provides a method for producing a cell block comprising the step of mixing and then curing the composition for producing a cell block comprising a water and / or PBS solution. When mixed with the cell population, the temperature of the cell block preparation composition is preferably 30 to 38 ℃. The cell block manufacturing method according to this process can be easily understood based on Preparation Example 2 below (see FIG. 8).

As used herein, a cell population is interpreted to refer to a cell population containing one or more cells. For example, a cell population obtained by centrifugation or the like of cells which are difficult to be cultured in a dish or the like from a cell suspension (passage cultured cells) is referred to.

According to the present invention, after culturing in a culture dish, the cells attached to the bottom of the dish can be immobilized without a separate desorption process to produce a cell block, and in the case of cells difficult to be cultured in a dish, centrifugation from a cell suspension (passage cultured cells) Only cells may be collected by immobilization and then immobilized to produce cell blocks. At this time, it is possible to minimize the cell separation process such as the use of reagents such as trypsin used for cell desorption or centrifugation, thereby reducing cell denaturation and damage.

In addition, according to the present invention, by stacking the cell layer in a predetermined direction, it is possible to produce a cell block to fix the cells in the desired direction in the cell block, so that the cell fragments can be observed by cutting the cell block in the desired direction.

In one embodiment of the present invention, the block in the gel state for cell injection may be colored as necessary so that the cells to be transplanted can be more clearly identified. The coloring of the gel block for cell injection can be easily carried out by those skilled in the art by dropping any color ink that does not chemically react with sugars, sodium chloride or agarose before the block is cured.

In preparing the cell block according to the present invention, the cells may be used by immobilization in advance according to methods known in the art. For example, cells collected from a cell or cell suspension on a culture dish are fixed with 4% formalin or alcohol, deformed formalin or alcohol with 1 × PBS (phosphate buffer), and cells removed with 1 × PBS removed, taking care not to dry the cells. use.

In the present invention, by using a solution containing a saccharide and sodium chloride as a gel molded body for cell block production, not only the cell block molding is easy but also cell modification by controlling water activity can be minimized. The present invention is not limited to a particular theory, but is based on thermodynamic principles that take into account changes in water activity with temperature and solute.

그래프 참조). 그러나, 이러한 수용액이 높은 온도에 있을 경우, 낮은 온도일 때보다 수분 함유량이 낮게 증가하다가 어느 시점(Inversion point)에서는 더 이상 수분 활성도가 증가하지 않게 된다(도 1의

그래프 참조). 수분 활성도가 낮으면 수분 함유도가 낮으므로 결정 상태의 설탕 등은 매우 적은 양의 용매(예: 물)와도 잘 흡착되어 세포의 형태를 유지하면서 세포와 밀착된다.1 and 2 will be described schematically in the implementation principle of the present invention. If the sugar or sodium chloride-containing aqueous solution such as sugar is at a low temperature, the slope of the graph gradually increases when the state changes from liquid to gas. The moisture content also increases (see FIG. 1).

See graph). However, when the aqueous solution is at a high temperature, the water content increases lower than at a low temperature, and at some point (inversion point), the water activity no longer increases (see FIG. 1).

See graph). When the water activity is low, the water content is low, so the sugar in the crystalline state is adsorbed well with a very small amount of solvent (eg, water) and adheres to the cell while maintaining the shape of the cell.

In the present invention, since the sugar and sodium chloride change considerably quickly from the amorphous state to the crystalline state according to the temperature change, it forms a crystalline membrane on the cell surface by crystallization within a short time when making the cell block, thereby lowering the water activity, and cell transformation after temperature change It serves to suppress (see Figure 2).

Therefore, according to the present invention, as described above, the sugar and sodium chloride rapidly change to a crystalline state according to the temperature change to form a crystalline membrane on the surface of the cell, so that the cells and agarose gel tightly adhered, the cell deformation after the temperature change Inhibition of the agarose gel and the cell-fixing agarose gel is in close contact with the cell block to cut the fragments of the fixing agarose gel and the cell is not separated (see Figure 3).

As shown in Figure 3, the gel containing sugars or sodium chloride and agarose is cooled to room temperature at a temperature of 50 ℃ or less while the sugar or sodium chloride dissolved in the solvent due to the temperature difference to form a thin crystalline membrane around the cells It forms and plays a role in minimizing cellular transformation due to temperature changes as well as osmotic pressure control (Sugar Affecting Hysteresis). That is, water molecules become relatively active at the temperature before the aqueous solution containing saccharides, sodium chloride and agarose in a gel state becomes relatively active, and when the solute reaches the saturation point of the solute, the solute is adsorbed around the cell. Rapid desorption by supersaturation of protects the appearance of the cells, which can prevent cell transformation and provide a more stable crystallization structure (see FIG. 3). On the other hand, the 1xPBS solution in which 1% agarose is dissolved in the conventional cell block is often transformed according to the temperature change that occurs during curing of the gel (see FIG. 4).

The third aspect of the present invention provides a cell block manufacturing kit comprising a composition for producing a cell block according to the present invention, a cell harvesting means, a cell block manufacturing frame, and instructions for use as necessary.

In one embodiment of the invention, the cell block preparation composition is a certain amount of agarose; Sugars, sodium chloride, or mixtures thereof; And packaged separately to include water and / or phosphate buffer solution (PBS), or in an amount of agarose; And sugars, sodium chloride, or mixtures thereof only, and a predetermined amount of water, 1 × PBS, or a mixed solvent thereof may be added separately in use.

Cell block manufacturing kit according to the present invention may be provided with instructions for use described as needed. Instructions for use describe in detail how to make cell blocks using the composition for making cell blocks. For example, the instructions for use include sugars, sodium chloride (NaCl), or mixtures and agarose in a solvent selected from water, phosphate buffer (PBS), and mixtures thereof, and the temperature at which sugars and agarose are dissolved. The cells block containing one or more cells is heated under the time that they are lysed, and then placed in a cell block manufacturing frame, and the cells collected by the cell harvesting means are injected into a cell block in a gel state molded in the cell block manufacturing frame. Processes for preparing the same may be described.

An example of a container including a composition for preparing a cell block is shown in FIG. 5. As shown in FIG. 5, the container 30 including the composition for preparing a cell block includes a first compartment 10 containing agarose, a second compartment 20 containing sugar and / or sodium chloride, Material that can be directly heated by mixing sugars and / or sodium chloride in the second compartment 20 and agarose in the first compartment 10 after use, and then adding a certain amount of water, 1 × PBS, or a mixed solvent thereof. It is preferable that it is the container 30 of. Optionally, the second compartment may optionally comprise an aqueous solution in which sugars and / or sodium chloride are dissolved in a predetermined amount of water or 1 × PBS in which they are dissolved. The material of the container constituting the first compartment and the second compartment may be the same or different.

The cell collecting means is not particularly limited as long as the cell collecting means includes a hollow tubular body capable of collecting cells therein and a discharge means capable of discharging the collected contents. An example of a cell harvesting means and method of use is shown in FIG. 6. The cell harvesting means consists of a hollow tubular body and an ejector for discharging the collected contents.

In the present invention, a cell block manufacturing frame may be used to mold the cell block into a predetermined shape. The form, material and the like are not particularly limited as long as they have at least one mold for forming the cell block shape for the cell block production. The cell block manufacturing frame may have a cover portion at the top of the compartment and a detachable slide plate at the bottom, and fixing means for fixing the slide plate to the mold, as necessary, so as to facilitate storage and separation of the cell block.

An example of a cell block manufacturing frame is shown in FIG. 7. As shown in FIG. 7, the cell block manufacturing frame includes one or more separate compartments 40 for cell block molding, a mold 50 having upper and lower openings, and a slide plate 60 disposed at the lower end of the compartments. ), The slit plate fixing means 70, the cover portion 80 of the mold. In an embodiment of the invention, the composition solution for cell block preparation according to the invention is poured into each of the one or more separate compartments 40, the cells collected by cell harvesting means before being fully cured and then cured and stored. When using the cell block, the fixing means 70 is released to remove the lower slide plate 60 and separate the cell block 90 from each compartment in the mold 50 (see FIG. 8). The separated cell block is cut in a desired direction by a cutting means such as a knife to obtain a section, and the cells contained in the section can be observed with a observation means such as a microscope.

In FIG. 7 and FIG. 8, the compartment 40 and the mold 50 are illustrated as hexahedrons, but their shapes may be manufactured in various shapes such as a polyhedron or a cylindrical shape including a hexahedron, and are not particularly limited.

The advantages of the case of preparing the cell block according to the present invention are as follows.

First, when the composition for producing a cell block according to the present invention is applied onto cells cultured in a culture dish (Fig. 9 (1)), the coated surface becomes nonuniform due to the presence of cells (Fig. 9 (2)). When the cells having this non-uniform coating surface are separated at least once by a cell collecting means such as a piston, cells stacked in multiple layers in the cell collecting means are obtained (Figs. 3 and 4 in Fig. 9), and they are completely cured. When injected into the composition gel for the preparation of untreated cell blocks (FIG. 9 (5)), the composition components, which have not yet been cured, are filled between the uneven cell layers stacked to fill the gap between the cells and the cell block gel and the cell chip Allow to be fixed to the cell block.

Secondly, according to the present invention, a cell block having a desired cell density can be obtained. By adjusting the cell density on the cell culture dish, the density of the cells collected by the cell harvesting means can be made large or small, and thus the cell density of the cell blocks prepared therefrom can also be freely controlled.

Third, since the cells can be stacked and injected into the cell block in a predetermined direction, the injected cells can be observed by cutting each region and angle (see FIG. 10). Therefore, the identification of intracellular components such as FISH (Fluorescence in situ hybridization) is not only easy, but after cell fragment preparation, the cell block gel is dissolved and removed, and then only the cells are separated and the cell fragments are collected to collect DNA, RNA, Proteins and the like. In addition, according to the present invention, the cell agglomerate is significantly less than that of the conventional agarose / 1xPBS solution, and there is little space between the cell and the cell block gel, thereby facilitating cell cleavage.

In the following, the invention is further described. However, this embodiment should not be construed as limiting the scope of the invention as defined in the following claims.

Example

Cell Block Preparation Example 1

An example of a cell block manufacturing method will be described with reference to FIG. 9.

Cells cultured in the culture dish were fixed with 4% formalin (room temperature, 20 minutes). Formalin was removed with lx PBS and lx PBS was removed (1), taking care not to dry the cells (1). Separately, 1 g agarose and 10 g sugar were added to 89 g of distilled water and boiled at 100 ° C. for 10 minutes to obtain a solution for preparing a cell block (not shown). The resulting solution was cooled to 37 ° C. and then thinly covered on a culture dish in which cells were fixed with a pipette (2). When the solution solidified to some extent in a gel state, cells were individually taken off using a straw piston (4) and removed (4). Step (4) can be repeated several times to uniformly stack several cells. Separately, a few drops of black ink was added to the remaining solution in the cell block preparation solution prepared above, and then colored in a block preparation frame. The stacked cells were injected into the straw piston before the solution in the block preparation mold solidified (5).

As shown in Fig. 10, in order to observe the cells injected into the cell block, the cell block obtained by cutting means such as a knife is cut in a desired direction to obtain a section, and the cells contained in the section are observed by a microscope or the like. Observe.

Cell Block Preparation Example 2

An exemplary cell block manufacturing method will be described with reference to FIG. 11.

In the case of cells that do not grow by attaching to the culture dish, a cell block may be prepared by collecting only cells with a centrifuge. 4% formalin was added to the cells to be observed to fix the cells (room temperature, 20 minutes). The immobilized cell-containing solution was centrifuged to discard the supernatant and to obtain the lower fixed cells. 1x PBS buffer was added to the fixed cells, washed well, and washed (1). The washed cells were centrifuged to discard the supernatant and to collect the cells again (indicated by arrows). Separately, 1 g agarose and 10 g sodium chloride (purified salt) were added to 89 g of distilled water and boiled at 100 ° C. for 10 minutes to obtain a solution for preparing a cell block (not shown). The obtained solution was cooled to 37 ° C., and then the cells obtained above were added and then injected into the cell block frame (2). The cells were cut and observed in the direction to be observed (3).

Claims (18)

0.5 to 2 weight percent agarose; 5-20% by weight of sugars, sodium chloride (NaCl) or mixtures thereof; And a solvent selected from the balance of water, phosphate buffer (PBS), and mixtures thereof. The composition of claim 1, wherein the cells are prokaryotic or eukaryotic cells. The composition of claim 1, wherein the saccharide is selected from monosaccharides, disaccharides, or polysaccharides. The composition of claim 1, wherein the sugar is at least one sugar selected from glucose, fructose, sucrose, maltose, lactose, cellulose, starch, and glycogen. The composition of claim 1, wherein the solvent is water. A method for producing a cell block from a cell line or cell suspension using the cell block preparation composition according to any one of claims 1 to 5. (a) applying the composition for producing a cell block according to claim 1 on cultured cells,
(b) collecting at least one or more cells to which the composition has been applied by using a cell collecting means, and
(c) injecting the collected cells into the cell block gel obtained by molding the cell block composition, cell block manufacturing method. A method of producing a cell block comprising the step of mixing the composition for producing a cell block according to claim 1 to a cell population comprising one or more cells and then curing. The method of claim 7 or 8, wherein the temperature of the composition for producing a cell block is 30 to 38 ℃ cell block manufacturing method. The method for producing a cell block according to claim 7 or 8, wherein the composition for producing a cell block is colored. The method of claim 7 or 8, wherein the cells are immobilized with formalin or alcohol in advance before being harvested. Composition for producing a cell block according to any one of claims 1 to 5; Cell harvesting means; Frameworks for making cell blocks; And kits for making cell blocks comprising instructions for use. The composition of claim 12, wherein the composition for making a cell block is contained in a heatable container comprising a first compartment comprising a predetermined amount of agarose and a second compartment comprising a predetermined amount of sugar, sodium chloride, or mixtures thereof. In use, agarose of the first compartment and sugars of the second compartment, sodium chloride, or a mixture thereof are mixed, followed by addition of a predetermined amount of water, a phosphate buffer (PBS), and a solvent selected from the mixture thereof. Will be a cell block manufacturing kit. The method of claim 12, wherein the cell block manufacturing composition comprises a first compartment containing a predetermined amount of agarose, and water in which a predetermined amount of sugar, sodium chloride, or a mixture thereof is dissolved, phosphate buffer (PBS), and their The cell block manufacturing kit which is contained in the heatable container containing a 2nd compartment containing a mixture. 13. The cell block manufacturing kit according to claim 12, wherein the cell collecting means includes a tubular body having a cavity to collect cells therein and an ejecting means for discharging the collected contents. The cell block manufacturing kit of claim 12, wherein the cell block manufacturing frame includes a cell block molding mold including one or more compartments. The method of claim 16, wherein the cell block manufacturing frame further comprises a removable cover portion on the upper part of the cell block molding mold, a removable slide plate on the bottom, and fixing means for fixing the slide plate to the mold. Kit for making cell blocks. The method of claim 12, wherein the instructions for use are added to a solvent selected from water, phosphate buffer (PBS), and mixtures thereof, sugars, sodium chloride (NaCl), or a mixture thereof and agarose, the sugars and agarose Under the temperature of lysis, they are heated for the time they are dissolved, and then placed in a cell block preparation frame, and the cells collected by the cell harvesting means are injected into a cell block in a gel state molded in the cell block preparation frame to include one or more cells. A cell block manufacturing kit, wherein the method for producing a cell block is described.

Images