KR101367780B1 - Manufacturing method of a suture for oriental medicine - Google Patents

Abstract

The present invention relates to a method for manufacturing a suture for oriental medicine and a suture manufactured thereby, and more particularly, to a suture which can stimulate a meridian system maximally and can be easily inserted into a human body by providing a separated stem to both ends of a monofilament. Moreover, the suture can reduce sterilization time and damage to products. [Reference numerals] (S1) Step for manufacturing a monofilament formed in a core-sheath shape with a diameter of 0.0001mm-10mm by complex spinning using two kinds of biodegradable polymers; (S2) Step for preventing a protrusion to give a multi-point stimulator effect by including a prickle shape on the surface through giving an effect of sheath monofilaments; (S3) Step for twisting the monofilament to two strands, three strands, or four strands, five strands and attaching the same; (S4) Step for inserting one strand monofilament or twisted monofilament to the needle of a disposable syringe without lubricating oil or coating the twisted monofilament with lubricating oil and inserting the twisted monofilament into the needle of a disposable syringe; (S5) Step for putting the monofilament bonded with the syringe needle in a Tyvek or sterilization paper for packaging; (S6) Step for sterilizing packaged products with E.O gas or H2O2 gas plasma

Description

Manufacturing method of a suture for oriental medicine

The present invention is a biodegradable polymer polydioxanone (poly p-dioxanone, also referred to as polydioxanone, abbreviated as PDO) from USP 3 & 4 to 9-0. By spinning or by hollow hollow fiber with one type of biodegradable polymer, another component of biodegradable material is introduced into the monofilament, which can stimulate the acupuncture points differently due to the difference in stiffness and difference in rate of degradation. In order to give a multi-point stimulator effect to the monofilament, it forms spines in various shapes on the surface of the monofilament to form protrusions, and then twists two, three, four strands or five strands. By combining, the monofilament can be used to stimulate more menstrual blood as well as to give a multi-point stimulator effect to the monofilament. By providing a branched stem with a straight branch divided into the branch, it is possible to maximize the meridians by giving a wide variety of stimulation points, and the two-, three-, four-, or five-stranded monofilament using a biodegradable material Coated and inserted into the syringe using a variable loop type tool, it is easy to insert into the syringe of both ends of the monofilament split, as well as by inserting the rod into the human body from the needle, Easy to insert into human body In addition, by introducing plasma hydrogen peroxide sterilization, the sterilization time can be drastically reduced, and the conventional long EO sterilization time can be shortened to prevent the product properties from dropping. to be.

Pharmacopuncture (藥 鍼) is a herbal treatment that is injected through acupuncture and drug action by injecting drugs into acupuncture points. Immunology (免疫 醫學) as one of the ingredients extracted from the herbal medicine to refer to the important acupuncture points (經穴). Acupuncture points are effective only by acupuncture stimulation, but they can be injected with certain drugs to achieve greater therapeutic effects. It is being researched and developed in various countries where oriental medical treatments are prevalent, such as Korea, China, and Japan.In Korea, the Korean Academy of Oriental Medicine has formed the Korean Academy of Herbal Acupuncture, and has been actively researching and distributing herbal acupuncture. It is a trend.

Acupuncture is a surgical therapy that stimulates the human body from the outside, and drug therapy is a medical therapy that internally stimulates the internal organs. Typically, acupuncture includes acute inflammatory diseases, chronic wasting diseases, various neuralgia, arthralgia, low back pain, musculoskeletal disorders such as fifty shoulders, various respiratory disorders, gastroenteritis such as gastroenteritis, neuropsychiatric disorders such as urinary disorders, neurodebilitating chronic headaches, It is used for the treatment of various dermatological diseases, hypertension and tumors.

Recently, it is also used for the cosmetic purpose of stimulating acupuncture points by using oriental medicine chambers (sometimes called chambers).

For such treatment, in recent years, biodegradable surgical sutures have been injected to give continuous stimulation to menstrual blood, giving continuous stimulation during biodegradation to increase the treatment. However, the product used in this treatment has the disadvantage that the irritation is relatively weak because it uses the form of a monofilament of a simple shape having a smooth surface or a product that provides a simple scratch or embossing effect on the surface of the monofilament.

Therefore, in recent years, multi-point stimulator method has been introduced and used to give more stimulation to acupuncture points. The multi-point stimulator is a product in which a variety of possible stimulation points are generated to maximize the meridians. That is, it is a product in which various stimulation points are created to maximize the stimulation on the surface, the sock end, and the inside of the biodegradable material, and a multi-point stimulator is realized, which uses one strand or a plurality of strands. Can be used as twisted. In addition, the composite yarn inserting heterogeneous biodegradable polymers in various forms inside the monofilament or hollow fiber is introduced therein. In this way, the stimulus can be given differently by changing the decomposition rate inside.

However, the multi-point stimulator sutures in the monofilament is very difficult to insert into the needle. In addition, when the product of the monofilament having a large number of magnetic poles is inserted into the needle, there is a problem that the visible shapes are easily damaged.

In addition, in the conventional E.O sterilization (Ethylene Oxide or less E.O) method, E.O sterilization has a problem that the sterilization time is very long, resulting in poor physical properties of the product.

In addition, the herbal chamber manufacturing method (Korean Patent Registration Publication No. 10-1180446; 2012.08.31) has been registered, but the herbal chamber manufacturing method has a scratch or embossing effect on the surface to stimulate more acupuncture points Although an improved invention, there is a problem that the method of inserting a simple scratched two strand braided product into a needle is not easy.

KR 10-2006-0122231 (2006. 12. 05)

Accordingly, the present invention was conceived to solve the above problems, and is a biodegradable polymer polypidioxanone (poly p-dioxanone hereinafter, referred to as PDO) from USP 3 & 4 to 9-0. By spun composite or by spinning hollow hollow fiber with one type of biodegradable polymer, another component of biodegradable material is introduced inside the monofilament to stimulate the acupuncture points differently due to differences in stiffness and degradation rate It is an object of the present invention to provide a method for manufacturing herbal chambers.

Another object of the present invention is to form a thorn in various shapes on the surface of the monofilament in order to give a multi-point stimulator effect on the monofilament to make a protrusion and then twist the two strands, three strands, four strands or It is to provide a method of manufacturing a herbal chamber that can stimulate acupuncture points by preparing by twisting five strands.

Still another object of the present invention is to provide a branched stem divided by linear branches at both ends of the monofilament to give a multi-point stimulator effect on the monofilament, giving a wide variety of stimulation points to maximize the meridians It is to provide a method of manufacturing a herbal chamber.

Another object of the present invention is to coat bi-, tri-, 4-, or 5-strand monofilaments with biodegradable materials, bond them together, and then insert them into a syringe using a variable loop tool, thereby splitting both ends of the mono It is to provide a method for manufacturing a herbal chamber that can be easily inserted into the syringe of the filament.

Another object of the present invention is to provide a method for manufacturing a herbal chamber for the insertion of the push rod into the human body in the needle, easy to insert into the human body of the split monofilament.

Another object of the present invention is to introduce a plasma hydrogen peroxide sterilization, not only can significantly reduce the sterilization time, but also to shorten the existing long EO sterilization time to prevent the product properties from falling product damage chambers To provide a method of manufacturing.

Method for producing a herbal chamber in accordance with a preferred embodiment of the present invention for achieving the above object is glycolide (glycolide), electide (L-lactide), dilactide (D-lactide), dielactide (D, Polymerization of L-lactide, p-dioxnaone, epsilon-caprolactone, and trimethylene carbonate alone, of glycolides and ellactide Combination copolymerization, combination copolymerization of L-lactide and epsilon-caprolactone, or two kinds of combination copolymerization and three kinds of combination copolymerization of the above seven monomers, Select one type to use biodegradable polymer 1 as core, and select one type from the above polymers and use biodegradable polymer 2 as sheath, that is, core-she composite spinning using two kinds of biodegradable polymers. preparing a monofilament having an ath shape and having a diameter of 0.0001 mm to 10 mm (S1); The monofilament has a sheath effect, and the visible shape protruding from the surface is linear along the monofilament stem, non-uniform linear form, rotates around the stem uniformly or non-uniformly, and has several branches at both ends of the monofilament. It provides projections that can give multi-point stimulator effects, including visible shapes in the form of stems or rotated 180 ° along the surface of the monofilament or 360 ° rotated along the stem surface. Step S2; Twisting the monofilament into two strands, three strands, or four strands and five strands by twisting and attaching the monofilament (S3); Inserting the monofilament into the disposable needle using the single-stranded or twisted monofilament without lubricating oil or lubricating the twisted monofilament into the disposable needle (S4); .

In the present invention, the step (S5) and put into the Tyvek (Syvek) or sterilized paper produced by combining with the needle after the step (S4); Sterilizing the packaged product with EO gas or sterilizing with plasma of H ₂ O ₂ gas (S6); It characterized in that it further comprises.

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In the present invention, in the step (S4), the lubricant is characterized in that using a silicone resin oil.

In the present invention, in the step (S6), the sterilization gas is EO gas, the concentration of the EO gas is 99% to 1% using 100% or mixed with CO ₂ gas, sterilization temperature is 10 ℃ to 100 ℃, sterilization time is characterized in that 1 minute to 36 hours.

In the present invention, in the step (S6), the sterilization gas is H ₂ O ₂ gas, the concentration of hydrogen peroxide gas is used in 99% to 1% by using 100% or mixed with water or other solvents And, the dose is 0.1mL to 1000mL, the sterilization method using a plasma, sterilization temperature is 0 ℃ to 100 ℃, sterilization time is characterized in that 1 minute to 36 hours.

In the present invention, after assembling the barbed monofilament to the syringe and assembling the rod at the end of the syringe is characterized in that the barbed form of the filamentary monofilament is characterized in that it is manufactured in a close-fitting structure that can be well put into the human body.

In addition, the manufacturing method of the herbal chamber in accordance with a preferred embodiment of the present invention for achieving the above object is glycolide (glycolide), elactide (L-lactide), dilactide (D-lactide), dielactide (D Polymerization of L-lactide, p-dioxnaone, epsilon-caprolactone, and trimethylene carbonate alone, with glycolide and l-lactide Combination copolymerization, combination copolymerization of L-lactide and epsilon-caprolactone, or two kinds of combination copolymerization and three kinds of combination copolymerization of the above seven monomers, and a diameter of 0.0001 mm to 10mm, spinning in the form of hollow hollow fibers to produce a monofilament (T1); The hollow fiber monofilament has a sheath effect and is produced in the form of a thorn protruding from the surface, or the shape of the protruding thorn, and the end of the sock is made in the form of a thorn or protruding thorns, using one strand or twisting a plurality of The shape to be produced or the protruding thorn shape is straight along the monofilament stem, non-uniformly straight, rotated about or uniformly around the stem, rotated 180 ° along the stem or 360 ° along the stem (T2) giving a protrusion that can give a multi-point stimulator effect, including a visible form of the form; Inserting the monofilament into the needle without lubricating oil or by applying lubricating oil to the monofilament (T3); Putting the monofilament prepared in combination with the needle into a Tyvek or a sterile paper and packaging it (T4); Sterilizing the packaged product with EO gas or sterilizing with plasma of H ₂ O ₂ gas (T5); .

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In the present invention, in the step (T3), the lubricating oil is characterized in that using a silicone resin oil.

In the present invention, in the step (T5), the sterilization gas is EO gas, the concentration of the EO gas is 99% to 1% using 100% or mixed with CO ₂ gas, sterilization temperature is 10 ℃ to 100 ℃, sterilization time is characterized in that 5 minutes to 36 hours.

In the present invention, in the step (T5), the gas to be sterilized is H ₂ O ₂ gas, the concentration of hydrogen peroxide gas is used in 99% to 1% by using 100% or mixed with water or other solvents And, the sterilization method using a plasma, sterilization temperature is 0 ℃ to 100 ℃, sterilization time is characterized in that 1 minute to 36 hours.

In the present invention, after assembling the barbed monofilament to the syringe after assembling the push rod at the end of the syringe is characterized in that the barbed monofilament is characterized in that it is manufactured in a rod-like structure that can be well put into the human body.

In addition, the manufacturing method of the herbal chamber in accordance with a preferred embodiment of the present invention for achieving the above object is glycolide (glycolide), elactide (L-lactide), dilactide (D-lactide), dielactide (D Polymerization of L-lactide, p-dioxnaone, epsilon-caprolactone, and trimethylene carbonate alone, with glycolide and l-lactide Combination copolymerization, combination copolymerization of L-lactide and epsilon-caprolactone, or two kinds of combination copolymerization and three kinds of combination copolymerization of the above seven monomers, Spinning using one biodegradable polymer to produce monofilaments having a diameter of 0.0001 mm to 10 mm (U1); The monofilament has a sheath effect, so that the visible shape protruding from the surface is linear along the monofilament stem, non-uniform linear form, rotates uniformly or non-uniformly around the stem, rotates 180 ° along the stem, or Giving a protrusion capable of giving a multi-point stimulator effect, including a visible shape rotating 360 ° along the stem, and simultaneously cutting and cutting the both ends of the monofilament with a knife (U2); Inserting the monofilament into the needle without lubricating oil or lubricating the monofilament into the disposable needle (U3); Assembling (U4) the barbed monofilament inserted into the needle by using a push rod to facilitate the injection into the human body; Putting the monofilament prepared in combination with the needle into a Tyvek or sterilized paper and packaging (U5); Sterilizing the packaged product with EO gas or sterilizing with plasma of H ₂ O ₂ gas (U6); .

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In the present invention, in the step (U3), the lubricating oil is characterized in that using a silicone resin oil.

In the present invention, in the step (U6), the sterilization gas is EO gas, the concentration of the EO gas is 99% to 1% using 100% or mixed with CO ₂ gas, sterilization temperature is 10 ℃ to 100 ℃, sterilization time is characterized in that 1 minute to 36 hours.

In the present invention, in the step (U6), the gas to be sterilized is H ₂ O ₂ gas, the concentration of hydrogen peroxide gas is used in 99% to 1% by using 100% or mixed with water or other solvents And, the dose is 0.1mL to 1000mL, the sterilization method using a plasma, sterilization temperature is 0 ℃ to 100 ℃, sterilization time is characterized in that 1 minute to 36 hours.

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The manufacturing method of the herbal chamber according to the present invention has the following effects.

First, the present invention is a biodegradable polymer polypidioxanone (hereinafter referred to as PDO) from USP 3 & 4 to 9-0, which is a composite spinning in the form of core-sheath with another polymer or one biodegradable By spinning hollow hollow fibers with polymers, biodegradable materials of different components are introduced into the monofilament, which can stimulate menstrual blood differently due to differences in stiffness and differences in degradation rates.

Secondly, the present invention forms a thorn in various shapes on the surface of the monofilament in order to give a multi-point stimulator effect to the monofilament, and then creates two protrusions, three strands, four strands or 5 By twisting the strands to make them, more menstrual blood can be stimulated.

Third, the present invention can provide a variety of stimulation points to give a multi-point stimulator effect on the monofilament, the branched branch divided by a straight branch at both ends of the monofilament, it is possible to maximize the meridians.

Fourth, the present invention is to coat the two-, three-, four- or five-stranded monofilament with a biodegradable material, and then insert into the syringe using a variable loop tool, so that both ends of the monofilament It is easy to insert into the syringe.

Fifth, the present invention is inserted into the human body by the introduction of a push rod, it is easy to insert into the human body of the split monofilament both ends.

Sixth, the present invention can not only significantly reduce the sterilization time by introducing plasma hydrogen peroxide sterilization, but also shorten the existing long E.O sterilization time to prevent the physical properties of the product to reduce the damage of the product.

1 to 3 is a flow chart for manufacturing a herbal chamber according to an embodiment of the present invention.
4a and 4b are photographic views showing two strands of monofilament assembled into a needle in accordance with one embodiment of the present invention;
FIG. 5 is a photograph showing a needle and a monofilament in which both ends are split and a multi-point stimulator is introduced to the surface according to an embodiment of the present invention.
Figure 6 is a photograph showing a monofilament in which both ends are split and a multi-point stimulator is introduced to the surface according to an embodiment of the present invention.
7 is a photograph showing a three-stranded monofilament in which both ends are split and a multi-point stimulator is introduced on a monofilament surface according to an embodiment of the present invention.
8 is a cross-sectional view of a core-sheath according to an embodiment of the present invention.
9 is a cross-sectional view showing a hollow yarn according to an embodiment of the present invention.
10 is a view showing a monofilament of a single component having both ends divided according to an embodiment of the present invention and a protrusion added to a surface thereof.
Figure 11 is a view showing a hollow fiber monofilament is split both ends, the projection is added to the surface according to an embodiment of the present invention.
12 is a view showing a core-sheath monofilament having both ends divided according to an embodiment of the present invention and a protrusion added to a surface thereof.
13 is a view showing a push rod combined with a needle according to an embodiment of the present invention.
14 is a diagram illustrating PDO open circuits into which various types of multi-point stimulators are introduced according to an embodiment of the present invention;
15 is a view showing the needle coupled to the herbal chamber using a variable loop type tool according to an embodiment of the present invention.
FIG. 16 is a photographic diagram illustrating a herbal chamber prepared by inserting one strand of biodegradable material to which a multi-point stimulator is applied in a needle half and then rotating the rest around the needle in a rotatable manner according to an embodiment of the present invention.
17 is an enlarged photographic view of a herbal chamber prepared by inserting one strand of biodegradable material to which a multi-point stimulator is applied in a needle half and then rotating the remainder around the needle in a rotatable manner according to an embodiment of the present invention. .
FIG. 18 is a photographic diagram illustrating a herbal chamber prepared by half-inserting two strands of a biodegradable material to which a multi-point stimulator is applied according to an embodiment of the present invention in a needle, and then rotating the remainder around the needle.
19 is an enlarged photographic view of a herbal chamber prepared by inserting two biodegradable materials having a multi-point stimulator applied thereto into a needle half and then rotating the rest around the needle in a rotatable manner. .

Looking at the preferred embodiment of the present invention together with the accompanying drawings as follows, when it is determined that the detailed description of the known art or configuration related to the present invention may unnecessarily obscure the subject matter of the present invention The description will be omitted, and the following terms are defined in consideration of functions in the present invention, which may vary depending on the intention or custom of the user or operator, and the definition thereof is a method of manufacturing a herbal chamber of the present invention and its manufacture It should be made based on the contents throughout the specification to describe the herbal chambers.

Hereinafter, with reference to the accompanying drawings, a method for manufacturing a herbal chamber and the prepared herbal chamber according to an embodiment of the present invention will be described in detail.

1 to 3 is a flow chart for manufacturing a herbal chamber according to an embodiment of the present invention.

As shown in Figure 1, look at the manufacturing process of the herbal chamber as follows.

First, as a step of preparing a monofilament, glycolide (L-lactide), di-lactide (D-lactide), dielactide (D, L-lactide), pydioxanone ( p-dioxnaone, epsilon-caprolactone, and trimethylene carbonate polymerized alone, a combination copolymerization of glycolide and l-lactide, and lactide -lactide) and epsilon-caprolactone in combination copolymerization, or two kinds of combination copolymerization and three kinds of combination copolymerization of the above seven monomers, and one of the above polymers is selected to select a biodegradable polymer 1 It is used as a core, and it is a core-sheath type that uses one type among the above polymers and uses biodegradable polymer 2 as sheath, that is, composite spinning using two kinds of biodegradable polymers, and the diameter is 0.0001mm to 10mm. The.

The second step is to give a protrusion to the monofilament, to give a sheath effect to the monofilament to give a multi-point stimulator effect of various forms, including the visible form on the surface to protrude in various forms To grant. Here, the visible shape of the surface is a protruding spine shape along the monofilament stem in a straight form, a non-uniform linear form, a form that rotates around the stem uniformly or non-uniformly, a branch of several at both ends of the monofilament It is made in the form made or rotated 180 ° along the surface of the monofilament or made in the form of 360 ° rotated along the stem surface.

The third step is to attach the monofilament, twisting the monofilament into two, three, or four and five strands with twist.

Fourthly, the monofilament is inserted into the disposable needle, and the twisted monofilament is inserted into the disposable needle without lubricating oil, or the twisted monofilament is lubricated and inserted into the disposable needle. Here, the lubricating oil is to use a silicone resin oil.

Fifthly, the monofilament is packaged, and the monofilament prepared by combining with the needle is packaged in Tyvek or sterilized paper.

Sixthly, the monofilament is sterilized, and the packaged product is sterilized by EO gas or by plasma of H ₂ O ₂ gas.

Here, the concentration of the sterilized EO gas is used 100% or mixed with CO ₂ gas 99% to 1%, sterilization temperature is 10 ℃ to 100 ℃, sterilization time is 1 minute to 36 hours. In addition, the concentration of the H ₂ O ₂ gas to be sterilized using 100% or mixed with water or other solvents to use 99% to 1%, the dosage is 0.1mL to 1000mL, sterilization using plasma The sterilization temperature is 0 ℃ to 100 ℃, sterilization time is 1 minute to 36 hours.

As shown in Figure 2, look at the manufacturing process of the herbal chamber as follows.

First, as a step of preparing a monofilament, glycolide (L-lactide), di-lactide (D-lactide), dielactide (D, L-lactide), pydioxanone ( p-dioxnaone, epsilon-caprolactone, and trimethylene carbonate polymerized alone, a combination copolymerization of glycolide and l-lactide, and lactide -lactide) and epsilon-caprolactone in combination copolymerization, or by using two kinds of combination copolymerization and three kinds of combination copolymerization of the above seven monomers, and the diameter of 0.0001mm to 10mm, hollow hollow fiber It is to spin in form to produce a monofilament.

Secondly, as a step of giving a protrusion to the monofilament, a protrusion that can give a multi-point stimulator effect of various forms including a visible form on the surface to give a sheath effect to the hollow fiber monofilament to protrude in various forms To give a part. Here, the visible form of the surface is to be produced in a protruding visible form. In addition, the protruding spine shape is a straight form along a monofilament stem, a non-uniform linear form, rotated around the stem uniformly or non-uniformly, rotated 180 ° along the stem or rotated 360 ° along the stem will be.

Thirdly, the monofilament is inserted into the disposable needle, and the monofilament is inserted into the disposable needle without lubricating oil, or the monofilament is lubricated and inserted into the needle. Here, the lubricating oil is to use a silicone resin oil.

Fourthly, the monofilament is packaged, and the monofilament produced by combining with the needle is packaged in Tyvek or sterilized paper. Here, after assembling the barbed monofilament to the syringe is assembled to the end of the syringe to be manufactured in a rod-like coupling structure that can be inserted into the body of the visible monofilament well.

Fifthly, the monofilament is sterilized, and the packaged product is sterilized with EO gas or with plasma of H ₂ O ₂ gas. Here, the concentration of the sterilized EO gas using 100% or CO ₂ 99% to 1% by mixing with gas, sterilization temperature is 10 ℃ to 100 ℃, sterilization time is 5 minutes to 36 hours. In addition, the concentration of the H ₂ O ₂ gas to be sterilized using 100% or mixed with water or other solvents to use 99% to 1%, sterilization using a plasma, sterilization temperature is 0 ℃ To 100 ° C., and the sterilization time is 1 minute to 36 hours.

As shown in Figure 3, look at the manufacturing process of the herbal chamber as follows.

First, as a step of preparing a monofilament, glycolide (L-lactide), di-lactide (D-lactide), dielactide (D, L-lactide), pydioxanone ( p-dioxnaone, epsilon-caprolactone, and trimethylene carbonate polymerized alone, a combination copolymerization of glycolide and l-lactide, and lactide -lactide) and epsilon-caprolactone in combination copolymerization, or by using two kinds of combination copolymerization and three kinds of combination copolymerization of the above seven monomers, and using one type of biodegradable polymer of the above polymer Spinning, the diameter is 0.0001mm to 10mm.

The second step is to give a protrusion to the monofilament, to give a sheath effect to the monofilament to give a multi-point stimulator effect of various forms, including the visible form on the surface to protrude in various forms At the same time, both ends of the monofilament is cut with a knife to give a split effect. In this case, the visible shape of the surface is a straight shape along the monofilament stem, a non-uniform linear form, a form that rotates around the stem uniformly or non-uniformly, a 180 ° rotational form or stem along the stem Therefore, it is rotated 360 °.

Thirdly, the monofilament is inserted into the disposable needle by inserting the monofilament into the needle without lubricating oil or by lubricating the monofilament into the disposable needle. Here, the lubricating oil is to use a silicone resin oil.

Fourth, assembling the monofilament using a straw, it is to assemble using a straw to facilitate the injection of the visible monofilament inserted into the needle to the human body.

Fifthly, the monofilament is packaged, and the monofilament prepared by combining with the needle is packaged in Tyvek or sterilized paper.

Sixthly, the monofilament is sterilized, and the packaged product is sterilized by EO gas or by plasma of H ₂ O ₂ gas. Here, the concentration of the EO gas to be sterilized using 100% or mixed with CO ₂ gas is 99% to 1%, sterilization temperature is 10 ℃ to 100 ℃, sterilization time is 1 minute to 36 hours. In addition, the concentration of the H ₂ O ₂ gas to be sterilized using 100% or mixed with water to use 99% to 1%, the dosage is 0.1mL to 1000mL, sterilization using a plasma, sterilization temperature Is 0 ° C to 100 ° C, and the sterilization time is 1 minute to 36 hours.

4A and 4B are photographic views showing two strands of monofilaments assembled to a needle according to an embodiment of the present invention, and FIG. 5 is a needle according to an embodiment of the present invention, and both ends before being inserted into the needle. This split is a photograph showing a monofilament in which a multi-point stimulator is introduced to a surface, and FIG. 6 shows a monofilament in which both ends are split and a multi-point stimulator is introduced into a surface according to an embodiment of the present invention. FIG. 7 is a photographic view showing three strands of monofilament having both ends split and a multi-point stimulator introduced on a monofilament surface according to an embodiment of the present invention.

As shown in Figure 4a to 7, in order to give a multi-point stimulator effect on the monofilament, to form a protrusion in the shape of various shapes on the surface of the monofilament to make a protrusion (11) and then two strands, It can be manufactured by twisting three strands or four strands or by twisting five strands to further stimulate the acupuncture points, as well as providing a branched stem 12 having straight branches divided at both ends of the monofilament. Giving the stimulus point is to maximize the meridians.

8 is a view showing a cross-section of the core-sheath according to an embodiment of the present invention, Figure 9 is a view showing a cross section of the hollow yarn according to an embodiment of the present invention, Figure 10 is an embodiment of the present invention Fig. 11 is a view showing a monofilament of a single component having both ends divided and a protrusion added to the surface thereof, and FIG. 11 is a hollow fiber having both ends divided according to an embodiment of the present invention and a protrusion formed on the surface thereof. FIG. 12 is a view illustrating a monofilament, and FIG. 12 is a view illustrating a core-sheath monofilament having both ends split and a protrusion added to a surface thereof according to an embodiment of the present invention.

As shown in FIGS. 8 to 12, USP 3 & 4 to 9-0 to introduce different biodegradable substances into the monofilament to differently stimulate menstrual blood due to stiffness difference and decomposition rate difference. Biodegradable polymer polypolydioxanone (hereinafter referred to as PDO) is a complex spinning in the form of core-sheath with other polymers, or spinning hollow hollow fiber using one biodegradable polymer. .

13 is a view showing a push rod combined with a needle according to an embodiment of the present invention.

As shown in FIG. 13, in order to facilitate insertion of the monofilament having both ends separated into the human body, a push rod 30 is introduced and inserted into the human body from the needle 20.

FIG. 14 is a diagram illustrating PDO open circuits into which various types of multi-point stimulators are introduced according to an embodiment of the present invention.

As shown in FIG. 14, in order to give a multi-point stimulator effect to the monofilament, the protrusions 11 are formed by forming a spine in various shapes on the surface of the monofilament.

15 is a view showing a needle coupled to a herbal chamber using a variable loop type tool according to an embodiment of the present invention.

As shown in FIG. 15, two-, three-, four-, or five-stranded monofilaments are coated and bonded with a biodegradable material in order to facilitate insertion into the syringe of bifinished monofilaments. It is inserted into the syringe using the variable loop tool 40.

16 is a photographic view showing a herbal chamber prepared by inserting one strand of biodegradable material to which a multi-point stimulator is applied in a needle half and then rotating the remainder around a needle in accordance with an embodiment of the present invention. FIG. 17 is a photograph of an enlarged herbal medicine chamber prepared by inserting one strand of biodegradable material to which a multi-point stimulator is applied in a needle half and then rotating the wound around the needle in a rotatable manner according to an embodiment of the present invention. Drawing.

As shown in Fig. 16 and 17, the herbal chamber is manufactured by inserting one strand of the biodegradable material to which the multi-point stimulator is applied into the needle, and then rotating the rest around the needle.

FIG. 18 is a photographic view showing a herbal chamber prepared by inserting two biodegradable materials having a multi-point stimulator applied thereto into a needle half and then rotating the rest around the needle in a rotatable manner. 19 is an enlarged photograph of a herbal chamber prepared by half-inserting two strands of a biodegradable material to which a multi-point stimulator is applied according to an embodiment of the present invention into a needle, and then rotating the rest around the needle. Drawing.

As shown in Fig. 18 and 19, the herbal chamber is prepared by half-inserting two strands of the biodegradable material to which the multi-point stimulator is applied, and then rotating the rest around the needle.

<Examples>

Example 1 relates to the preparation of a core-sheath composite biodegradable monofilament, polypidiooxane (Polyp-) having an intrinsic viscosity of 2.6 dL / g (measured with a 30 ° C. 0.1 g / dL HFIP solution, referred to as HFIP below Hexafluoroisopropanol) dioxanone) polymer chip and poly e-caprolactone polymer chip having an intrinsic viscosity of 2.0 dL / g (measured with a 0.1 g / dL HFIP solution at 30 ° C. and below Hexafluoroisopropanol, referred to as HFIP) according to Table 1 Composite spinning produced 0.129 mm USP 6-0 in diameter. At this time, the inner core was made of polyepsilon caprolactone, and the outer sheath was made of polydioxanone. The biodegradable suture yarn was used to produce a thorn-like protrusion 11 on the foliage at regular intervals using a thorn generator. Braided sutures were prepared by twisting two strands using this surface protruding biodegradable yarn. Loctide 4014, a two-strand twister suture biodegradable adhesive, was used to bond each end to every 20 mm, and was cut in units of 60 mm. The twister suture thus prepared was sandwiched between disposable syringe needles and fixed with a sponge 21. The herbal herbal medicines prepared were put in primary Tyvek sterile paper and sealed by heat-sealing the packaging material with a tower packaging machine. Subsequently, it was put into an aluminum pouch for the second time, and prepared for E.O sterilization (hereinafter referred to as ethylene oxide, E.O).

PDO-PCL Compound Spinning Conditions Item Polydioxanone
Condition Polycaprolactone
Condition Extruder Screw RPM 7.5 7.5 Tip Pressure (Kgf / cm ² ) 80 85 Extruder temperature
(℃) 1 zone 155 160 2 zones 158 165 3 Zone 160 170 Nozzle Pack Temperature (℃) 160 170 Cooling tank temperature (℃) 23 Primary roller speed (m / min) 5.5 Primary oven temperature (℃) 80 Secondary roller speed (m / min) 35 Second oven temperature (℃) 85 3rd roller speed (m / min) 35 3rd oven temperature (℃) 90 Total draw ratio 6.2

Example 2 relates to the production of PDO biodegradable monofilament, polydioxane (Polyp-dioxanone) polymer chip having an intrinsic viscosity of 2.6 dL / g (measured with 0.1 g / dL HFIP solution at 30 ° C., referred to as HFIP below Hexafluoroisopropanol) The composite was spun in the method of Table 1 to prepare a diameter 0.129 mm USP 6-0. The biodegradable suture yarn was made using a spine generating device to produce a leafy spine protrusion at regular intervals. Braided sutures were prepared by twisting two strands using this surface protruding biodegradable yarn. Loctide 4014, a two-strand twister suture biodegradable adhesive, was used to bond both ends to every 20 mm and cut in units of 60 mm. The twister suture thus prepared was sandwiched between disposable syringe needles and fixed with a sponge 21. The herbal herbal medicines prepared were put in primary Tyvek sterile paper and sealed by heat-sealing the packaging material with a tower packaging machine. Subsequently, it was put in an aluminum pouch for the second time, and prepared for E.O sterilization (hereinafter referred to as ethylene oxide, E.O).

PDO emission conditions Item Polydioxanone
Condition Extruder Screw RPM 7.5 Tip Pressure (Kgf / cm ² ) 80 Extruder temperature
(℃) 1 zone 155 2 zones 158 3 Zone 160 Nozzle Pack Temperature (℃) 160 Cooling tank temperature (℃) 23 Primary roller speed (m / min) 5.5 Primary oven temperature (℃) 80 Secondary roller speed (m / min) 35 Second oven temperature (℃) 85 3rd roller speed (m / min) 35 3rd oven temperature (℃) 90 Total draw ratio 6.2

Example 3 was prepared in the same manner as in Example 1 PDO monofilament in the form of cobra barbed on the surface. Only one strand of a monofilament prepared by dividing both ends of the biodegradable suture yarn USP 6-0 with a blade in quarters was prepared. Thereafter, as shown in FIG. 15, the variable loop type tool 40 was used to insert the tip of the needle 20. Thereafter, to prepare E.O sterilization in the same manner as in Example 1.

Example 4 was prepared in the same manner as in Example 3. Only braided sutures were prepared by twisting two strands of biodegradable suture yarn USP 6-0. The hot wire was heated to perform instant bonding. E.O sterilization was performed in the same manner as in Example 1 to prepare biodegradable needles for cosmetic, cosmetic, and herbal medicine.

Example 5 relates to hollow fiber spinning conditions, in which the nozzle is specially designed as shown in FIGS. 4A and 4B with a Polyp-dioxnanoe polymer chip (measured by a 2.7 dL / g at 30 ° C. and 0.1 g / dL HFIP solution). A cross section of one type (the hollow lozenge of the spinning nozzle) is attached to the After inserting and assembling, the hollow undrawn yarn was spun by spinning with the mono spinning machine under the conditions of Table 3. PDO hollow fiber (USP 3-0) was prepared by extending the hollow unstretched yarn 1-4 times after one day storage in a nitrogen chamber at room temperature. Braided sutures were prepared by twisting two strands using this biodegradable hollow fiber yarn. Loctide 4014, a two-strand twister suture biodegradable adhesive, was used to bond the sock end every 20 mm and cut in 60 mm increments. The sutures thus prepared were sandwiched between the syringe needles and fixed with a sponge 21. The herbal herbal medicines prepared were put in primary Tyvek sterile paper and packed in a tower packaging machine. It was then put into an aluminum pouch for the second time, and prepared for EO sterilization.

PDO emission conditions Item Polydioxanone
Condition Extruder Screw RPM 7.5 Tip Pressure (Kgf / cm ² ) 80 Extruder temperature
(℃) 1 zone 155 2 zones 158 3 Zone 160 Nozzle Pack Temperature (℃) 160 Cooling tank temperature (℃) 23

Example 6 is a monomer of PGCL (Poly glycolide-co-epsilon caprolactone, wherein the molar ratio of glycolide and epsilon caprolactone is 75:25) polymer chip (IV = 1.6 dL / g, measured with 0.1 g / dL HFIP solution at 30 ° C.). USP 6-0 of 0.124 mm was prepared under the conditions of Table 4 using a spinning machine. Braided sutures were prepared by twisting two strands using this biodegradable suture yarn. The two-strand twister suture biodegradable adhesive Loctide 4014 was used to bond the sock end every 20 mm and cut in 60 mm units. The sutures thus prepared were sandwiched between the syringe needles and fixed with a sponge 21. The herbal herbal medicines prepared were put in primary Tyvek sterile paper and packed in a tower packaging machine. Subsequently, it was put into an aluminum pouch and subjected to E.O sterilization. Subsequently, a secondary aluminum wrapper was packed. This prepared a biodegradable needle for herbal medicine. In addition, by twisting three strands, four strands or five strands in the same manner to prepare a biodegradable needle for herbal medicine.

PGCL emission conditions Item Condition Extruder Screw RPM 7.5 Tip Pressure (Kgf / cm ² ) 80 Extruder temperature
(℃) 1 zone 235 2 zones 240 3 Zone 240 Nozzle Pack Temperature (℃) 240 Cooling tank temperature (℃) 23 Primary roller speed (m / min) 5.5 Primary oven temperature (℃) 80 Secondary roller speed (m / min) 35 Second oven temperature (℃) 85 3rd roller speed (m / min) 40 3rd oven temperature (℃) 90 Total draw ratio 7.2

Hereinafter, the physical properties of the chambers according to the above examples are shown in Table 5.

Comparison of Physical Property Results after Manufacturing Example diameter
(mm) Early EP Knot
strong
(Kgf / cm ² ) Initial EP Knot Strength (%) Strong knot after 2 weeks
(Kgf / cm ² ) EP knot strength after 2 weeks (%) EP knot strength after 4 weeks
(Kgf / cm ² ) EP knot after 4 weeks
strong(%) Example 7 0.129 0.59 100 0.430 86 0.39 84 Example 12 0.124 0.72 100 0.144 20 - - Example 13 0.124 0.72 100 0.180 25 - -

In the physical property measurement method, diameters were measured, EP knots were measured, and knot strength retention was measured.

1) The diameter measurement method was measured by the USP 921 method of the US Food and Drug Administration, and the measuring device was measured by applying tension to the suture of the multifilament using Mitutoyo dial-gaze. In addition, the suture thread was measured once and then rotated at an angle of 90 °, and ten measurements were taken as average values.

2) The method of measuring EP knot was knotted once with a surgical suture using Instron's tensile strength device, and the average value of 5 strands was determined by EP simple knot method.

3) Knot strength retention method was measured the initial EP knot strength of the manufactured suture. Then 1M phosphate buffered saline solution was prepared to pH 7.4. Insert the suture about 2m into the test tube and fill the 1M phosphate buffered saline solution to the suture. After adjusting the temperature of the shaking bath to 37 ° C., the prepared test tube was inserted and taken out after 14 or 28 days, and the knot strength retention was calculated by measuring the EP knot strength.

Examples 7,8,9,10,11,12 correspond to Examples 1,2,3,4,5,6 in this order, and relate to an EO sterilization method, Examples 1,2,3,4, The products in 5 and 6 were packaged in aluminum pouches with the top open and then in Tyvek. Placed in EO sterilizer chamber, humidified at 6 0 ± 10 RH% for 3 hours, decompressed to -760mmHg, sterilized for 4 hours at 0.8kg pressure with 20% EO gas (80% is CO ₂ gas). Proceeded. The product was taken out after 12 hours of aeration. Then, hot air dried at 45 ° C. for 4 hours, and then placed in a vacuum glove box, followed by vacuum drying at 45 ° C. for 12 hours. In a vacuum glove box, an aluminum pouch was sealed in a nitrogen atmosphere to prepare herbal chambers for cosmetic, molding, and oriental medicine. At this time, the moisture of the final product was 50ppm.

Example 13 relates to a H ₂ O ₂ plasma sterilization method, in the preparation of Example 6, instead of Tyvek sterile paper was placed in the Tyvek for plasma and sealed. The product was put into the plasma sterilizer as it was without packaging the aluminum pouch and plasma sterilization was performed. That is, after sterilization, the product was put in an aluminum pouch, and the aluminum pouch was sealed after vacuum drying. This plasma sterilization operation was performed by plasma sterilization using Renosem Plasma Sterilizer (RENO-S30).

Running a self-test program on an empty chamber initially reduces the chamber pressure to 1 Torr or less, then returns the chamber pressure to atmospheric pressure. At this time, check the preheating status, operation status of each part and whether leakage occurs in vacuum, and generate an error in case of abnormality, and display the “Self-Test Complete” screen during normal operation. Renosem plasma sterilizers always maintain temperatures below 55 ° C.

After normal operation, the plasma sterilizer door is opened, the suture product is placed in the plasma sterilizer chamber, the sterilant is placed in the sterilant inlet, and the door is closed. Select the sterilization cycle of the plasma sterilizer and press “Start” to operate as follows.

1) Initializing stage

Decompression-decompression was repeated inside the chamber using a vacuum pump, and the sterilant was reached to 1 Torr or less versus the last decompression day before being introduced into the chamber. Decompression enters the chamber by cleanly filtering outside air through the HEPA filter. This process removes and warms up the internal suture product.

2) Sterilizing I Stage

Hydrogen peroxide 50wt% 1-5mL is injected into the chamber through the vaporizer, the pressure in the chamber rises to diffuse. The injected sterilizer is condensed by rising to atmospheric pressure or above atmospheric pressure by the HEPA filter. At this time, the plasma generated by the external DBD (Dielectric Barrier Discharge) plasma is supplied into the chamber and sterilized by hydrogen peroxide and plasma. . The pressure was again reduced by the vacuum pump to reach 1 Torr or less. During vacuum decompression, the Corona plasma connected to the front of the vacuum pump is activated to decompose hydrogen peroxide into water and oxygen through the vacuum pump.

3) Sterilizing II Stage

Hydrogen peroxide 50wt% 1 ~ 5mL is injected into the chamber through the vaporizer, the pressure in the chamber rises to diffuse. The injected sterilizer is condensed by rising to atmospheric pressure or above atmospheric pressure by the HEPA filter. At this time, the plasma generated by the external DBD plasma is supplied into the chamber and sterilized by hydrogen peroxide and plasma.

4) Finishing Stage

The pressure was again reduced by the vacuum pump to reach 1 Torr or less. At this time, the Corona plasma connected to the front end of the vacuum pump is operated to decompose hydrogen peroxide with water and discharge through the vacuum pump. And the clean air supplied by the HEPA filter releases the pressure to atmospheric pressure. If decompression-decompression is done again, the sterilization process is completed. Plasma sterilization was performed for a total of 60 minutes.

Example 14 relates to various visualization methods. In order to implement various multi-point stimulators, the surface of a monofilament was implemented using various blades to form a surface like T in FIG. 14A. They embodied the visible shape in one direction or 180 ° or 360 ° direction of the monofilament. In addition, the two ends were divided into two pieces by sheaths, or divided into 3-10 pieces.

 As described above, the method for producing herbal chambers of the present invention can be widely applied to the fields of improved cosmetic, cosmetic and herbal biodegradable acupuncture.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the invention. It is not.

10: chamber 11: protrusion
12: branched stem 20: needle
21: sponge 30: pushpin
40: variable loop tool

Claims (21)

In the manufacturing method of the herbal chamber,
Glycolide, L-lactide, D-lactide, Di-lactide, P-dioxnaone, epsilon-caprolactone , Polymerized with trimethylene carbonate alone, combination copolymerization of glycolide and ellactide, and L-lactide and epsilon-caprolactone Combination copolymerization or two kinds of copolymerization and three kinds of combination copolymerization of the above seven monomers are used, and one type is selected from the above polymers, and the biodegradable polymer 1 is used as the core, and one type is selected from the above polymers. Using a biodegradable polymer 2 as a sheath, i.e., preparing a monofilament having a core-sheath shape of composite spinning using two kinds of biodegradable polymers, having a diameter of 0.0001 mm to 10 mm (S1);
The monofilament has a sheath effect, and the visible shape protruding from the surface is linear along the monofilament stem, non-uniform linear form, rotates around the stem uniformly or non-uniformly, and has several branches at both ends of the monofilament. It provides projections that can give multi-point stimulator effects, including visible shapes in the form of stems or rotated 180 ° along the surface of the monofilament or 360 ° rotated along the stem surface. Step S2;
Twisting the monofilament into two strands, three strands, or four strands and five strands by twisting and attaching the monofilament (S3);
Inserting the monofilament into the disposable needle using the single-stranded or twisted monofilament without lubricating oil or lubricating the twisted monofilament into the disposable needle (S4); Method of manufacturing a herbal chamber, characterized in that it comprises a. The method of claim 1,
After the step (S4) and the monofilament prepared by combining with the needle in Tyvek (Syvek) or sterilized paper step (S5) and packaging;
Sterilizing the packaged product with EO gas or sterilizing with plasma of H ₂ O ₂ gas (S6); Method for producing a herbal chamber, characterized in that it further comprises. delete The method of claim 1,
In the step (S4), the lubricating oil is a method of manufacturing a herbal chamber, characterized in that using a silicone resin oil. 3. The method of claim 2,
In the step (S6), the sterilization gas is EO gas, the concentration of the EO gas is used 99% or 1% by using 100% or mixed with CO ₂ gas, sterilization temperature is 10 ℃ to 100 ℃, Sterilization time is a method of producing a herbal chamber, characterized in that 1 minute to 36 hours. 3. The method of claim 2,
In the step (S6), the gas to be sterilized is H ₂ O ₂ gas, the concentration of hydrogen peroxide gas is used in 99% to 1% by using 100% or mixed with water or other solvents, the dosage is 0.1mL To 1000mL, the method of sterilization using a plasma, sterilization temperature is 0 ℃ to 100 ℃, sterilization time is 1 minute to 36 hours manufacturing method of a herbal chamber, characterized in that. In the manufacturing method of the herbal chamber,
Glycolide, L-lactide, D-lactide, Di-lactide, P-dioxnaone, epsilon-caprolactone , A mixture of trimethylene carbonate alone, a combination copolymerization of glycolide and ellactide, and a combination of ellactide and epsilon-caprolactone (T1) to prepare a monofilament by copolymerizing, or by using two kinds of combination copolymerization and three kinds of combination copolymerization of the above seven monomers, and having a diameter of 0.0001 mm to 10 mm and spinning in hollow hollow fiber form (T1) ;
The hollow fiber monofilament has a sheath effect and is produced in the form of a thorn protruding from the surface, or the shape of the protruding thorn, and the end of the sock is made in the form of a thorn or protruding thorns, using one strand or twisting a plurality of The shape to be produced or the protruding thorn shape is straight along the monofilament stem, non-uniformly straight, rotated about or uniformly around the stem, rotated 180 ° along the stem or 360 ° along the stem (T2) giving a protrusion that can give a multi-point stimulator effect, including a visible form of the form;
Inserting the monofilament into the needle without lubricating oil or by applying lubricating oil to the monofilament (T3);
Putting the monofilament prepared in combination with the needle into a Tyvek or a sterile paper and packaging it (T4);
Sterilizing the packaged product with EO gas or sterilizing with plasma of H ₂ O ₂ gas (T5); Method of manufacturing a herbal chamber, characterized in that it comprises a. delete delete 8. The method of claim 7,
In the step (T3), the lubricating oil is a method of manufacturing a herbal chamber, characterized in that using a silicone resin oil. 8. The method of claim 7,
In the step (T5), the gas to be sterilized is EO gas, the concentration of the EO gas is 99% to 1% using 100% or mixed with CO ₂ gas, sterilization temperature is 10 ℃ to 100 ℃, Sterilization time is a manufacturing method of the herbal chamber, characterized in that 5 minutes to 36 hours. 8. The method of claim 7,
In the step (T5), the gas to be sterilized is H ₂ O ₂ gas, the concentration of hydrogen peroxide gas is used in 99% to 1% by using 100% or mixed with water or another solvent, using a plasma Method of sterilization by, sterilization temperature is 0 ℃ to 100 ℃, sterilization time is a method for producing a herbal chamber, characterized in that 1 minute to 36 hours. 8. The method of claim 1 or 7,
After assembling the barbed monofilament to the syringe assembly of the push rod at the end of the syringe is a method of manufacturing a herbal chamber, characterized in that the barbed monofilament is manufactured in a push-bond structure that can be well put into the human body. In the manufacturing method of the herbal chamber,
Glycolide, L-lactide, D-lactide, Di-lactide, P-dioxnaone, epsilon-caprolactone , A mixture of trimethylene carbonate alone, a combination copolymerization of glycolide and ellactide, and a combination of ellactide and epsilon-caprolactone A monofilament having a diameter of 0.0001 mm to 10 mm was prepared by spinning with one copolymer of two or three kinds of copolymerization of the above seven monomers and using one biodegradable polymer of the above polymer. Step (U1);
The monofilament has a sheath effect, so that the visible shape protruding from the surface is linear along the monofilament stem, non-uniform linear form, rotates uniformly or non-uniformly around the stem, rotates 180 ° along the stem, or Giving a protrusion capable of giving a multi-point stimulator effect, including a visible shape rotating 360 ° along the stem, and simultaneously cutting and cutting the both ends of the monofilament with a knife (U2);
Inserting the monofilament into the needle without lubricating oil or lubricating the monofilament into the disposable needle (U3);
Assembling (U4) the barbed monofilament inserted into the needle by using a push rod to facilitate the injection into the human body;
Putting the monofilament prepared in combination with the needle into a Tyvek or sterilized paper and packaging (U5);
Sterilizing the packaged product with EO gas or sterilizing with plasma of H ₂ O ₂ gas (U6); Method of manufacturing a herbal chamber, characterized in that it comprises a. delete The method of claim 14,
In the step (U3), the lubricating oil is a method of manufacturing a herbal chamber, characterized in that using a silicone resin oil. The method of claim 14,
In the step (U6), the gas to be sterilized is EO gas, the concentration of the EO gas is 99% to 1% using 100% or mixed with CO ₂ gas, sterilization temperature is 10 ℃ to 100 ℃, Sterilization time is a method of producing a herbal chamber, characterized in that 1 minute to 36 hours. The method of claim 14,
In the step (U6), the gas to be sterilized is H ₂ O ₂ gas, the concentration of hydrogen peroxide gas is used in 99% to 1% by using 100% or mixed with water, the dosage is 0.1mL to 1000mL, Sterilization method using a plasma, sterilization temperature is 0 ℃ to 100 ℃, sterilization time is a method for producing a herbal chamber, characterized in that 1 minute to 36 hours. delete delete delete

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