KR101360219B1 - Composition for attracting and preventing Synanthedon tenuis (Butler) - Google Patents

Abstract

The present invention relates to a male pheromone male attracted and controlled sex pheromone composition using a sex pheromone component secreted by the chick larvae female and a method for producing the same. More specifically, the composition for a baby glass moth attractant comprising (Z, Z) -3,13-octadecadienol and (E, Z) -2,13-octadecadienol as an active ingredient and pheromone using the same A trap and a mating disturbance agent are disclosed. Through the composition according to the present invention it is possible to produce a pheromone trap, which is a tool for generating forecasting and mass shedding, and furthermore can be produced by the mating disturbance to reduce the damage of persimmon caused by baby glass moth environmentally friendly.

Description

Composition for attracting and controlling baby glass moths {Composition for attracting and preventing Synanthedon tenuis (Butler)}

The present invention relates to a baby glass moth attraction and control composition.

Baby Glass Moth Synanthedon Tenuis (Butler), which occurs once a year, overwinters as a larva, and due to different developments in wintering larvae, adults develop all over May to October. Baby glass moth cools the formation layer under the bark, and in particular, the callus, which is the joint part of the grafting part, is vulnerable to breakage by wind, and the damage is often large. Spraying of insecticides may be used to prevent the damage of such pests. However, since insecticides act non-selectively on insects, even insects are killed, and the immunity to the overall pesticide is strengthened, thereby reducing the pest control effect. Accordingly, in order to selectively control only pests of interest, a method of attracting pests of interest with pheromones has been developed and used.

The use of sex pheromone is divided into developmental prediction using pheromone trap, direct control by mass killing and mating disturbance. Among these, the developmental prediction field continuously investigates the occurrence of pests and judges the need for pesticide treatment. In addition, it is possible to pinpoint the timing of pesticide treatment if necessary, which is the basis of comprehensive pest management that can prevent pesticide abuse and preserve the ecosystem.

On the other hand, it was known that the adult females of the baby glass moth males were attracted by (Z, Z) -3,13-octadecadienyl acetate ((Z, Z) -3,13-Octadecadienyl acetate) (TAMAKI, Y. , YUSHIMA, T., ODA, M., KIDA, K., KITAMURA, K., YABUKI, S., and TUMLINSON, HH 1977.Attractiveness of 3,13-octadecadienyl acetates for males of clearwing moths.Jap.J. Appl. Entomol. Zool. 21: 106-107.). However, no follow-up studies have been done and no effectiveness has been verified.

Therefore, it is urgent to identify the sex pheromone produced and released by the baby glass moth female, evaluate the attracting effect of the male adult to the composition of the identified component and utilize it for developmental prediction and environmentally friendly control.

An object of the present invention is to provide a composition capable of attracting and controlling baby glass moths.

The present invention relates to (Z, Z) -3,13-octadecadienol ((Z, Z) -3,13-Octadecadienol) and (E, Z) -2,13-octadecadienol ((E And Z) -2,13-Octadecadienol). In one embodiment of the present invention (Z, Z) -3,13-octadecadienol and (E, Z) -2,13-octadecadienol are in a ratio of 9: 1 to 1: 9 It is characterized by being mixed.

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In addition, in one embodiment of the present invention (Z, Z) -3,13-octadecadienol ((Z, Z) -3,13-Octadecadienol) and (E, Z) -2,13-octadeca Provided are baby glass moth attracting traps comprising dieneol ((E, Z) -2,13-Octadecadienol).

In addition, in one embodiment of the present invention (Z, Z) -3,13-octadecadienol ((Z, Z) -3,13-Octadecadienol) and (E, Z) -2,13-octadeca Provided is a composition for baby glass moth copulation agent containing dieneol ((E, Z) -2,13-Octadecadienol).

In addition, in one embodiment of the present invention, a baby glass moth comprising mixing (Z, Z) -3,13-octadecadienol and (E, Z) -2,13-octadecadienol Provided is a method of preparing an attractant or control composition.

In addition, in one embodiment of the present invention, a baby glass moth comprising mixing (Z, Z) -3,13-octadecadienol and (E, Z) -2,13-octadecadienol It provides a method for producing a composition for copulation disturbances.

Pheromone means a secreted compound that causes a social response to other individuals of the same species. Pheromones cause specific behaviors in individuals who have received the pheromones, and there are dangerous pheromones, food-induced pheromones, sex pheromones, and the like.

Sex pheromones are pheromones that indicate that females can reproduce. Many types of insects release sex pheromones to attract spouses. For example, Lepidoptera insects such as moths and butterflies can detect sex pheromones about 10 km away.

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In the present invention, the pheromone composition is used after being dissolved in a solvent, solvents that can be used in the present invention, but are not limited thereto, methane, ethane, propane, butane, isobutane, pentane, isopentane branched or unbranched alkanes groups such as tert-butane, hexane and sec-hexane; Ketone groups such as dimethyl ketone, ethyl methyl ketone and diethyl ketone; Alcohol groups such as propanol, butanol, isobutanol, pentanol, isopentanol; Carboxylic acid groups such as formic acid, acetic acid, propionic acid, butanoic acid and valeric acid; Aromatic groups such as benzene, toluene, imidazole and alkyl benzene or mixtures thereof.

Gas Chromatography Mass Spectrometer (GC-MS) is a device that combines the functions of gas chromatography and mass spectrometry and can analyze the compounds present in the mixture. After the organic matter is separated by gas chromatography, and then ionized by mass spectrometer ion source electron energy (70 eV), the generated ions are separated by the mass-to-charge ratio (m / z) at the quadrupole. By analyzing the relative intensities of ions and searching the library, the chemical structure, chemical reaction, and molecular weight of the separated material can be identified.

Through the baby glass moth attracting and controlling composition according to the present invention, by attracting the baby glass moth, it is possible to disturb the occurrence prediction, control and mating of the baby glass moth.

1 is a result of analyzing a compound of a female baby glass moth pheromone gland by a gas chromatography mass spectrometer according to an embodiment of the present invention.
2 is a photograph of a baby glass moth trapped in a sticky and attracted to the composition for baby glass moth attracting according to an embodiment of the present invention.

Hereinafter, the components and technical features of the present invention will be described in more detail with reference to the following examples. However, the following examples are intended to illustrate the contents of the present invention and are not intended to limit the scope of the invention.

Various embodiments described herein are described with reference to the drawings. In the following description, for purposes of complete understanding of the present invention, various specific details are set forth, such as specific forms, compositions, and processes, and the like. However, certain embodiments may be practiced without one or more of these specific details, or with other known methods and forms. In other instances, well-known processes and techniques of manufacture are not described in any detail, in order not to unnecessarily obscure the present invention. Reference throughout this specification to "one embodiment" or "embodiment" means that a particular feature, form, composition, or characteristic described in connection with the embodiment is included in at least one embodiment of the invention. Accordingly, the appearances of the phrase " in one embodiment "or" the embodiment "in various places throughout this specification are not necessarily indicative of the same embodiment of the present invention. In addition, a particular feature, form, composition, or characteristic may be combined in any suitable manner in one or more embodiments.

Example  One. Baby Glass Moth  female St. Pheromones  Identification of matter

Sex pheromone compounds were isolated from sex pheromone glands of A. erythema females, and pheromones contained in extracts of A. erythroid pheromone gland were identified using gas chromatography mass spectrometry (CG-MS).

As a result, as shown in FIG. 1, (Z, Z) -3,13-octadecadienol ((Z, Z) -3,13-Octadecadienol, hereinafter referred to as Z3, Z13-18: OH) and It was found that (E, Z) -2,13-octadecadieneol ((E, Z) -2,13-Octadecadienol, hereinafter referred to as E2, Z13-18: OH).

Example  2. Z3 , Z13 -18: OH  And E2 , Z13 -18: OH  Mixture of Baby Glass Moth  Attraction effect

Z3, Z13-18: OH and E2, Z13-18: OH identified in Example 1 alone or mixed in a ratio of 10: 0, 9: 1, 5: 5, 1: 9 or 0:10 We tried to lure the males of glass moths.

Experimental conditions are as follows.

The experiment was carried out in persimmon trees in Gimhae, Gyeongnam, the solvent was used hexane (hexane), and mixed by adding as much as the capacity shown in Table 1 to 100ul nucleic acid and then immersed in a rubber diaphragm to prepare bait. Each lure was inserted into a small wire in the center of a delta-shaped sticky trap (275mm-195mm-1170mm), and then the hook was hung on a persimmon tree branch about 1m high above ground and examined for male moths caught in the traps at weekly intervals.

Z3, Z13-18: OH (μg) E2, Z13-18: OH (μg) Yoo In Soo (Marie / Trap) Experimental Example 1 900 100 17.5 Experimental Example 2 500 500 5.0 Experimental Example 3 100 900 3.3 Comparative Example 1 1,000 0 0 Comparative Example 2 0 1,000 0 Control Example 0 0 0

As a result, as shown in Table 1, in the Comparative Example 1, Comparative Example 2 and the control example, the baby glass moth was not attracted at all. On the other hand, when the ratio of Z3, Z13-18: OH to E2, Z13-18: OH is from 9: 1 to 1: 9, the attracting effect on the baby glass moth was found, especially Z3. When the ratio of, Z13-18: OH to E2, Z13-18: OH is 9: 1, the attracting effect was excellent.

On the other hand, the attracting effect of (Z, Z) -3,13-octadecadienyl acetate ((Z, Z) -3,13-Octadecadienyl acetate), which is known as a attractant of baby glass moth, It did not appear to exist.

Example  3: Baby Glass Moth  Preparation of Pheromone Trap Using Attractant Composition

Rubber diaphragm by mixing a composition comprising (Z, Z) -3,13-octadecadienol and (E, Z) -2,13-octadecadienol, which are baby glass moth sex pheromone components When the bait deposited on the rubber septum was installed in the sticky trap, it was confirmed that the male baby glass moth was killed by the trap in large quantities.

Example  4: Baby Glass Moth St. Pheromones  Preparation and Control Effects of Coagulation Disturbants

Coagulation of a mixture of (Z, Z) -3,13-octadecadienol and (E, Z) -2,13-octadecadienol, which are the components of the baby glass moth sex pheromone, in a polyethylene tube When the disturbance agent was installed in the persimmon field, it was confirmed that normal mating behaviors of female and male baby glass moths were disturbed, thereby reducing persimmon damage caused by the next generation larvae.

Claims (11)

(Z, Z) -3,13-octadecadienol ((Z, Z) -3,13-Octadecadienol) and (E, Z) -2,13-octadecadienol ((E, Z) -2,13-Octadecadienol) Baby glass moth attracting composition comprising.
The method of claim 1,
(Z, Z) -3,13-octadecadienol and (E, Z) -2,13-octadecadienol are characterized in that the mixture in a weight ratio of 9: 1 to 1: 9 Baby glass moth attracting composition.
A composition for controlling a baby glass moth, comprising (Z, Z) -3,13-octadecadienol and (E, Z) -2,13-octadecadienol.
The method of claim 3, wherein
(Z, Z) -3,13-octadecadienol and (E, Z) -2,13-octadecadienol are characterized in that the mixture in a weight ratio of 9: 1 to 1: 9 Baby glass moth control composition. delete delete delete delete delete delete delete

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