KR101359349B1

KR101359349B1 - Nanowire complex and bio sensor using the same

Info

Publication number: KR101359349B1
Application number: KR20100044278A
Authority: KR
Inventors: 이민호; 정석원; 성우경
Original assignee: 전자부품연구원
Priority date: 2010-05-12
Filing date: 2010-05-12
Publication date: 2014-02-11
Also published as: KR20110124855A

Abstract

A nanowire composite and a biosensor using the same are provided. The present nanowire composite includes a nanowire and a protein containing solution attached to the surface of the nanowire to control the conductivity of the nanowire. As a result, the biosensor can be manufactured without the complicated chemical process and the attachment process to attach the protein to the nanowire surface, thereby reducing the manufacturing cost and effort.

Description

Nanowire complex and bio sensor using the same

The present invention relates to a biosensor, and more particularly to a biosensor used to detect a target protein.

Nano-sized small diameter materials have recently become very important because of their unique electrical, optical and mechanical properties.

The research on nanostructures that have been conducted so far shows new possibilities such as quantum size effects and the potential of new optical device materials in the future. In particular, nanostructures can be used not only as single electron transistor devices, but also as various chemical sensors and biosensors, and are attracting attention as new optical device materials. Here, the nanostructure is a concept including carbon nanotubes, nanorods and nanowires.

Biosensors based on nanosensors using nanowires or carbon nanotubes are chemical factors, biomolecules, and disease markers (enzymes, proteins, DNA, carbohydrates, nucleic acids, antigens) to be detected on receptors immobilized on the surface of nanostructures. , Antibodies, etc.) react specifically to the surface of the nanostructures, so that only specific molecules can be detected.

1A is a plan view illustrating a conventional biosensor. As shown in FIG. 1A, the conventional biosensor is implemented by connecting the nanowires 13 between the electrodes 11 and 12 formed at both ends of the substrate 10.

1B illustrates a cross-sectional view of the biosensor shown in FIG. 1A taken along the dashed-dotted lines A through B. As shown in FIG. 1B, the surface of the nanowire 13 functions as a receptor (receptor as a receptor). Y) is attached and fixed.

In the case of the conventional biosensor, it is difficult to process chemical groups on the surface of the nanowires 13, and it is difficult to stably attach the antibody to chemical groups fixed to the surface of the nanowires 13, and even if attached, they are stably attached. It is hard to expect.

In addition, after antigen-antibody binding, the measurement should be performed in a washing state, in which case there is a problem that the antibody-antigen binding is not fixed and is lost.

The present invention has been made to solve the above problems, an object of the present invention, a nanowire complex that does not require complex chemical process and attachment process that must be accompanied to attach a protein such as an antibody to the surface of the nanowire And to provide a biosensor applying the same.

Another object of the present invention is to provide a nanowire composite having a structure in which proteins are more stably attached to the nanowires so that proteins are not lost even when the external physical shock is applied, and a biosensor using the same.

According to the present invention for achieving the above object, a nano wire composite, nano wire; And a protein-containing solution attached to a surface of the nanowires to control conductivity of the nanowires.

The protein-containing solution is preferably a sol or gel solution.

Moreover, it is preferable that the said protein containing solution covers the whole surface of the said nanowire.

The protein-containing solution may control the conductivity of the nanowires according to the capture degree of the target protein.

In addition, if the target protein is an antigen, the protein contained in the protein-containing solution is an antibody, and if the target protein is an antibody, the protein contained in the protein-containing solution may be an antigen.

On the other hand, according to the present invention, a biosensor, a substrate; Electrodes formed on the substrate; A nano wire formed on the substrate and connecting the electrodes; And a protein-containing solution attached to a surface of the nanowires to control conductivity of the nanowires.

The biosensor may further include an injection unit providing a path through which the protein-containing solution is injected.

The biosensor may further include a delivery unit configured to provide a path through which the target protein is delivered to the protein-containing solution, wherein the protein-containing solution controls conductivity of the nanowires according to the capture degree of the target protein. Can be.

The protein-containing solution is preferably a sol or gel solution.

As described above, according to the present invention, it is possible to manufacture a biosensor without the complicated chemical process and attachment process that must be accompanied to attach a protein such as an antibody to the surface of the nanowire, thereby reducing the manufacturing cost and effort It becomes possible.

In addition, since the protein is more stably attached to the nanowires, the proteins are not lost due to an external physical impact, thereby enabling more accurate sensing.

Figure 1a is a plan view showing a conventional biosensor,
FIG. 1B is a cross-sectional view showing a cross section of the biosensor shown in FIG. 1A taken along dashed-dotted lines A to B;
2 illustrates a biosensor according to an embodiment of the present invention;
3A and 3B are cross-sectional views of the biosensor shown in FIG. 2 taken along dashed-dotted lines A through B;
4 illustrates a biosensor according to another embodiment of the present invention, and
5 is a view showing a more complete form of the biosensor.

Hereinafter, the present invention will be described in detail with reference to the drawings.

2 is a diagram illustrating a biosensor according to an embodiment of the present invention. As shown in FIG. 2, the biosensor 100 according to the present exemplary embodiment includes a substrate 110, a nanowire sol / gel composite 120, and electrodes 150 and 160.

As shown in FIG. 2, a first electrode 150 and a second electrode 160 are formed on the substrate 110. The nanowire sol / gel composite 120 is provided between the first electrode 150 and the second electrode 160.

The nanowire sol / gel composite 120 includes a nanowire 130 and an antibody-containing sol / gel 140. The nanowire 130 is a nanoscale wire having both ends electrically connected to the electrodes 150 and 160, respectively.

The antibody-containing sol / gel 140 is a sol or gel solution containing an antibody to detect a target antigen and is attached to the surface of the nanowire 130.

As shown in FIG. 2, the antibody-containing sol / gel 140 is attached to the surface of the nanowire 130 in a size that can cover the entire surface of the nanowire 130. In addition, the antibody-containing sol / gel 140 covers a part of the surface of the first electrode 150 and the second electrode 160.

Since the antibody contained in the antibody-containing sol / gel 140 binds to the target antigen and the antigen-antibody, the target antigen can be captured.

3A and 3B, cross-sectional views of the biosensor 100 shown in FIG. 2 taken along a dashed-dotted line (A-B) are shown. In FIG. 3A, antibodies contained in the antibody-containing sol / gel 140 are shown. The target antigens are not captured in (Y), and in FIG. 3B, the target antigens (O) are captured in the antibodies (Y) contained in the antibody-containing sol / gel 140.

When a target antigen is captured by an antibody contained in the antibody-containing sol / gel 140, an electric field is generated in the nanowire 130 to which the antibody-containing sol / gel 140 is attached due to the charge of the captured target antigen. The change in conductivity of the nanowires 130 is induced.

The amount of change in conductivity of the nanowires 130 depends on the amount of antibody captured by the antibody-containing sol / gel 140. This is because the amount of carrier of the nanowires 130 varies depending on the amount of antibody captured by the antibody-containing sol / gel 140.

In detail, when a large number of antigens are captured by antibodies contained in the antibody-containing sol / gel 140, the carriers of the nanowires 130 may increase, thereby increasing the conductivity of the nanowires 130. As a result, the amount of current flowing between the first electrode 150 and the second electrode 160 electrically connected to the nanowire 130 increases.

On the other hand, if fewer antigens are captured in the antibodies contained in the antibody-containing sol / gel 140, the carrier of the nanowire 130 is reduced, thereby lowering the conductivity of the nanowire 130. As a result, the amount of current flowing between the first electrode 150 and the second electrode 160 electrically connected to the nanowire 130 is reduced.

Therefore, when the amount of current flowing between the first electrode 150 and the second electrode 160 provided in the biosensor 100 is measured, the amount or density of the target antigen can be measured. Accordingly, the biosensor 100 may be used for medical purposes such as disease diagnosis and allergy diagnosis.

4 is a diagram illustrating a biosensor according to another embodiment of the present invention. As shown in FIG. 4, the biosensor 200 according to the present exemplary embodiment includes a substrate 210, a nanowire sol / gel composite 220, and electrodes 250, 260, and 270.

The biosensor 200 illustrated in FIG. 4 is a biosensor implemented in a FET method, and has a drain electrode 250, a source electrode 260, and a gate electrode 270. There is a difference from the biosensor 100 shown in FIG. 2 having 160.

In addition, the substrate 210 and the nanowire sol / gel composite 220 provided in the biosensor 200 illustrated in FIG. 4 may include the substrate 110 and the substrate 110 provided in the biosensor 100 illustrated in FIG. 2. Since the same device as the nanowire sol / gel composite 120, detailed description thereof will be omitted.

Figure 5 shows a more complete form of the biosensor. The biosensor illustrated in FIG. 5 is implemented by further including a microfluidic channel 300 in the biosensor 200 illustrated in FIG. 4. The microfluidic channel 300 includes a sol / gel injection unit 310 and a target antigen delivery unit 320.

The sol / gel injection unit 310 is a passage connected from the 'top surface of the microfluidic channel 300' to the 'top of the nano wire 230' and is used as a path for injecting the antibody-containing sol / gel 240. The antibody-containing sol / gel 240 injected through the sol / gel injection unit 310 is attached to the nanowires 130.

The target antigen delivery unit 320 is a passage used as a path for delivering a solution to detect whether the target antigen is included to the antibody-containing sol / gel 240. The solution injected into the inlet of the target antigen delivery unit 320 passes through the antibody-containing sol / gel 240 at the center of the target antigen delivery unit 320 and then flows out of the outlet.

Up to now, the nanowire composite and the biosensor using the antibody-containing sol / gel attached to the surface of the nanowire have been described in detail with reference to preferred embodiments.

In the present embodiment, it is assumed that the target antigen is detected using the antibody-containing sol / gel, but this is merely an example for convenience of description. In the case of detecting the target antibody using the antigen-containing sol / gel, the technical idea of the present invention can be applied. Furthermore, the present invention can be applied to detecting a target protein using a protein-containing solution other than an antigen or an antibody.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, It will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the present invention.

100, 200: biosensor
110, 210: substrate
120, 220: Nano wire sol / gel composite
130, 230: Nanowire
140, 240: antibody-containing sol / gel
150, 160, 250, 260, 270: electrode
300: microfluidic channel
310: sol / gel injection unit
320: target antigen delivery unit

Claims

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In the biosensor,
Board;
Electrodes formed on the substrate;
A nano wire formed on the substrate and connecting the electrodes; And
And a protein-containing solution attached to a surface of the nanowires to control conductivity of the nanowires.
The protein containing solution,
It is a sol or gel solution containing protein inside the solution,
An injection unit providing a path for injecting the protein-containing solution from the top of the biosensor; And
Further comprising: a delivery unit for providing a path for delivering the target protein to the protein-containing solution,
The electrodes include a drain electrode, a source electrode and a gate electrode, so that the biosensor is driven in a FET manner,
The path of the injection portion is perpendicular to the substrate,
The path of the said transmission part is a biosensor characterized by the parallel to the said board | substrate.

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The method according to claim 6,
The protein-containing solution, the biosensor characterized in that to control the conductivity of the nanowires according to the capture degree of the target protein.

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The method according to claim 6,
The protein containing solution,
A biosensor, covering the entire surface of the nanowires.