KR101352972B1 - Gynostemma pentaphyllum extracts compositions for treating or preventing inflammatory diseases - Google Patents

Abstract

The present invention relates to a composition for treating and preventing inflammatory diseases comprising Gynostemma pentaphyllum (Gynostemma pentaphyllum) extract as an active ingredient, more specifically, the actinogen (Arctigenin) in Chilbap extract as an indicator, the content is constant It is standardized and standardized to be included in the scope, and the suppressive effect of inflammatory changes such as analgesic suppression, acute inflammatory suppression and acute edema suppression and the like is excellently expressed, which is useful for treating and preventing diseases caused by inflammatory changes such as arthritis. The present invention relates to a chilbap extract which can be used as a medicament.

Description

Gynostemma pentaphyllum extracts compositions for treating or preventing inflammatory diseases, including extract of Chil-Yeop Dam (Ｇｙｎｏｓｔｅｍｍａ ｐｅｎｔａｐｈｙｌｌｕｍ) as an active ingredient

The present invention relates to a composition for the treatment and prevention of inflammatory diseases comprising the extract of Chil yew (Gynostemma pentaphyllum) as an active ingredient, more specifically, the standardization and so that the content of the actinigenin (Arctigenin) in chile yeast extract in a certain range It is standardized and can be used as an agent useful for the treatment and prevention of diseases caused by inflammatory changes such as arthritis due to the excellent expression of inhibitory effects caused by inflammatory changes such as analgesic suppression, acute inflammatory suppression and acute edema suppression. It is related to a bile extract.

Arthritis is a disease that is medically referred to as an inflammatory change in joints caused by certain causes such as bacteria or trauma. These arthritis is divided into acute and chronic.

Acute arthritis is classified as follows. ① serous arthritis (보 性): Usually caused by trauma (外傷), the cause is unknown, usually occurs in only one joint. ② serous fibrosis (奬 液 纖維素 性) arthritis: Occurs during acute arthritis of arthritis, turbid effusion in the joint cavity (渗出 液) stops. Fibrosis of the stomach (虚膜) and inflammation of the left side of the severe motion disorder is left. ③ purulent (化膿 性) arthritis: joints in the open window (開放 創) or gonorrhea, typhoid fever, scarlet fever, sepsis (敗血症) is a multiple show. Infants at 1 to 2 months of age cause dislocations that can not be treated because of severe bone injuries. In adults, periosteal osteomyelitis is a common cause of the eruption of the farmer and the pus into the joints, which is called secondary arthritis arthritis.

Chronic arthritis is classified as follows. ① Specific Inflammation: Tuberculosis, syphilis or middle-aged man has gouty arthritis due to many metabolic disorders of uric acid. ② Multiple arthritis: Chronic arthritis is caused by many arthritis, acute serous arthritis in the transition (이행 行), tuberculosis, syphilis, gonorrhea during the course of the multiple, one of the sepsis. It also includes arthritis, which is called Still's disease. ③ deformable osteoarthritis: bone or joint aging or trauma is the cause. ④ hemophilia (血友病 性) arthritis: when hemophilia is caused by bleeding in the joints.

Many medicines have been developed for the treatment of diseases caused by inflammatory changes represented by arthritis as described above.

On the other hand, Gynostemma pentaphyllum is called Chil-yeopdam in the name of the herbal medicine. It is also called Chil-Yeop Dam, Vine Tea, and Vine Ogapi. The leaves are regenerated and have multicellular white hairs on both sides, but soon disappear. The lobules are usually 5 but 3-7. The narrow ovate oval or narrow ovate. The leaflets are 4-8cm long and 2-3cm wide. Pointed tip with fine hairs on surface veins, serrated at edges.

Nodes have white hairs, grow tangled up and down, but climb up with tendrils. Flowers bloom in August-September, yellowish green, cone-shaped or gunshot cone-shaped, 8-15cm long. Calyx lobes are very small, corolla split into 5, lobes are 3mm long, lanceolate, and pointed long. The berry is 6-8mm in diameter, round, black-green mature, with one transverse line in the upper half, and the seed is 4mm long.

Root stem or starch (全 草) is an anti-inflammatory (해독), detoxification (解毒), sea water (멎) to the effect of removing the phlegm (효능) and the chronic bronchitis is a medicine to treat.

It is harvested in September-October, and its ingredients include sterols, sugars, pigments and glycosides.

In addition, the traditional Chinese medicine or related literature such as Dongbobom, Hyangjegbang, and light-dose class has been prescribed the above-mentioned Chilbap as a medicinal herb. However, these prescriptions are used to distinguish the shape of the Chilbapdam and its method of manufacturing herbal medicines and fluids. It was just a brief comment about. That is, no opinion on active active ingredients expressing the drug efficacy among the components extracted through the above-described methods could be obtained.

In addition, it is difficult to standardize extracts because Chil-Yeopdam has a large variation in the content of the indicator components in each place of origin and harvesting time, and commercialization is difficult because it cannot obtain reproducibly active fractions with excellent anti-inflammatory analgesic, improved blood circulation, and arthritis treatment. The fact remains a problem to be solved.

In order to solve the above problems of the inventors of the present invention, research to achieve the standardization of Chilchidam extract according to the specific components and its content control, as a result of suppressing the symptoms of inflammatory changes, anti-inflammatory analgesic action, The standardized chile chile extract, with excellent effects such as immune cell proliferation inhibitory effect, acute inflammation inhibitory, chronic inflammation inhibitory and acute edema inhibition, was completed with the present invention.

According to the results of the present invention, it was found that by standardizing to include a certain amount of actigenin in the chile chile extract, the chile chile extract can be easily and reproducibly obtained, and thus commercialized.

Therefore, an object of the present invention is to provide a pharmaceutical composition and a food composition for the treatment and prevention of inflammatory diseases comprising the chile chile extract as an active ingredient.

It is another object of the present invention to provide a extract of chile yeast extract, which is standardized so that the content of actinizenin is included as a specific range.

Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention, claims and drawings.

In order to achieve the above object, the present invention provides a pharmaceutical composition for the treatment and prevention of inflammatory diseases comprising the extract of Chil yeopdam (Gynostemma pentaphyllum) as an active ingredient.

In addition, the present invention is a pharmaceutical composition for the treatment and prevention of inflammatory diseases comprising Gynostemma pentaphyllum extract as an active ingredient, the actinigen (Arctigenin) as an indicator, the actinigenin (Arctigenin) 5.0 to 10.0 It provides a pharmaceutical composition for the treatment and prevention of inflammatory diseases, including chil yeopdam (G 葉 膽, Gynostemma pentaphyllum) extract prepared as an active ingredient.

In addition, the present invention is a method for producing chile chile extract, 1) extract by selecting one from the crowd consisting of water, alcohol, alcohol aqueous solution of 5 to 8 times the weight of the original medicine of Chile chile (Gynostemma pentaphyllum) And cooling and filtration, 2) separating the filtrate obtained above into an aqueous alcohol solution and concentrating under reduced pressure at 50 to 90 ° C., and 3) adding alcohol to the concentrate obtained above at 700 to 1,200 rpm. The centrifuged filtrate is concentrated under reduced pressure to provide a method for preparing chile chile extract comprising the step of preparing an extract containing 5.0 to 10.0% by weight of Actigenin as an indicator.

In addition, the present invention is a food composition for preventing inflammatory diseases comprising the extract of Chil yeopdam (Gynostemma pentaphyllum) as an active ingredient, using the actinogen (Arctigenin) as an indicator, the arctigenin (Arctigenin) is 5.0 To provide a food composition for preventing inflammatory diseases comprising chilebdam (七葉 膽, Gynostemma pentaphyllum) extract prepared to contain from 10.0% by weight as an active ingredient.

According to the present invention, Chilchidam extract was very effective in inhibiting inflammation in TPA-induced edema, arachidonic acid-induced edema, and carrageenan-induced acute foot edema, anti-inflammatory analgesic effect, articular tissue degrading enzyme inhibitory effect, immune cell proliferation inhibitory effect. , Acute inflammatory inhibitors, chronic inflammatory inhibitors and acute edema inhibitors are excellent, and standardization could be achieved by controlling the content of Actigenin as an indicator.

Therefore, Chilgapdam extract according to the present invention is extracted and formulated from Chilbapdam and selected as Actinogen (Arctigenin) as an indicator material and standardized by controlling the content of the indicator material prepared as an inflammatory disease treatment agent and health functional food such as arthritis treatment agent. can do.

In addition, the composition of the present invention is excellent in safety because it contains the chile yeopdam extract which has been used as a herbal medicine for a long time as an active ingredient.

Figure 1 shows the HPLC chromatogram of Chilbye extract.
Figure 2 is a TPA-induced ear edema inhibition test (TPA-induced mouse ear edema.) Of Chilbale extract.
3 is arachidonic acid-induced ear edema inhibition test (Arachidonic acid-induced mouse ear edema assay).
Figure 4 is an acetic acid-induced writhing response in mice.
Figure 5 is an acute arthritis treatment effect assay of chile chile extract.

Hereinafter, the present invention will be described in detail.

The present invention is standardized and standardized to include a certain amount of actinigen in the chile biloba extract to suppress the symptoms caused by inflammatory changes such as analgesic suppression, acute inflammatory suppression, chronic inflammatory suppression, acute edema suppression and chronic edema suppression The present invention relates to a chile chile extract, which has excellent effects and can be applied to drugs useful for the treatment and prevention of inflammatory diseases such as arthritis.

Since the content of the active bioactive substances contained in the original herbal medicine of Chilbapdam can vary greatly depending on the place of origin, harvesting time, storage period, and storage condition, the composition of each herbal composition should not be limited to the weight ratio of the ingredients used. It is more preferable to limit the content. In addition, there is a large difference in the joint protective physiological activity depending on the content of the effective physiologically active substance.

Therefore, in the present invention, as an indicator material for obtaining chile yeast extract maximizing the expression of drug efficacy, it has been previously selected no actinogen (Arctigenin). As an active ingredient of the medicament obtained in the present invention, the action of other components other than the above components cannot be excluded.

Chilgapdam extract used in the medicament for the treatment of diseases caused by inflammatory changes of the present invention can be selected by extracting the specific active fraction from the extract itself (medicinal extracts) or chilbipdam extract extracted from stone outpost. At this time, the compounding ratio is preferably determined by the content of the actinogen (Arctigenin) as an indicator material regardless of the content of the chil yeopdam, the original drug. Actigenin, which is the indicator, is preferably contained 5.0 wt% or more in the Chilbale extract, more preferably 5.0 to 10.0 wt%, even more preferably 5.0 to 8.25 wt%. Most preferably, it is contained by weight. If the arctigenin content is less than 5.0% by weight, relatively poor results for the treatment of arthritis and the like are obtained. The upper limit of the content of the Actigenin (Arctigenin) is not particularly limited, but if it contains more than a certain level of Actigenin (Arctigenin) does not increase the effect anymore, but also technical and economical to manufacture It tends to be undesirable in terms of aspect.

Actigenin (Arctigenin) selected as the indicator material in the present invention is an important component of the drug for treating diseases caused by inflammatory changes of the present invention, when contained in a certain content range can express a more potent drug effect by expressing a synergistic effect have. It is presumed to exhibit the therapeutic and prophylactic effect of inflammatory diseases by the action of other components in addition to Actigenin as the active ingredient of the chile chile extract obtained in the present invention. That is, it is impossible to exclude the action of other components other than the above-mentioned components as the active ingredient of the medicament obtained in the present invention.

The present invention provides a chile chile extract, characterized in that the extraction by selecting one from the crowd consisting of water, alcohol and alcohol aqueous solution.

The extract is,

Extracting, cooling, and filtering one from a crowd consisting of water, alcohol, and an aqueous alcohol solution of 5 to 8 times the weight of the crude medicine of Gynostemma pentaphyllum (七葉 膽, Gynostemma pentaphyllum),

Layering the filtrate obtained above into an aqueous alcohol solution and concentrating under reduced pressure at 50˜90 ° C., and

Concentrating the filtrate obtained by the addition of alcohol, centrifuged at 700 ~ 1,200 rpm and then concentrated under reduced pressure to prepare an extract containing 5.0 to 10.0% by weight of Actigenin as an indicator material.

Hereinafter, the method for preparing the extract will be described in detail.

1) Extracted, cooled, and filtered with 6-8 times the amount of water or alcohol aqueous solution of washed, dried and cut gallbladder protozoa, and 5 to 8 times the amount of mixed herbal medicine with water or alcohol solution was added to the residue. Filtering by warming and re-extracting, followed by filtration with a previous filtrate; 2) separating the filtrate obtained in 1) above with an equal volume of an alcohol aqueous solution and concentrating it under reduced pressure at 50 to 90 ° C, and 3) adding concentrated alcohol to the concentrate obtained in 2) Followed by centrifugation at 700 to 1,200 rpm, followed by concentration under reduced pressure, vacuum drying, grinding and sterilization.

To explain this in detail, washing and drying, adding water or alcohol aqueous solution to the fine Chilbale protozoa, extracted repeatedly for 2 to 4 hours, 2 to 4 times, slowly cooled to room temperature, and then centrifugal separation or the like. Filter and separate from the residue. Water or an aqueous solution of alcohol in an amount of 6 to 8 times the weight of the mixture is added to the residue, and the mixture is warmed to re-extract and then filtered and mixed with the previous filtrate. As described above, the extraction efficiency can be increased by adding water or alcohol aqueous solution to the residue and re-extracting and filtering.

At this time, if the amount of water or alcohol aqueous solution used for extraction is too small, the solubility of the extract is deteriorated to deteriorate the extraction efficiency. If the amount is too large, the amount of the aqueous alcohol solution used for layer separation increases, Which is not economical or may cause handling problems.

In the present invention, a method of re-extraction after primary extraction has been adopted. In the case of mass production of herbal medicine extracts, since the water content of the herbal medicine itself is high even if the filtration is effected effectively, the extraction efficiency is lowered only by the primary extraction alone This is to prevent this. In addition, it was found that 80 ~ 90% of the total extraction amount was extracted by the second extraction, and it is not economically feasible to extract more than third step.

As described above, the extract obtained by extracting with water or alcohol aqueous solution is filtered and concentrated, and unnecessary proteins, polysaccharides and fatty acids contained in the filtrate are purified. In the present invention, the same amount of alcohol The impurities are purified by separating the aqueous solution into two to five times the amount used as a solvent to obtain a solvent fraction.

The alcohol is preferably an alcohol having 1 to 6 carbon atoms, more preferably a 30% ethanol aqueous solution. When the amount of the lower alcohol aqueous solution used is less than the filtrate, fine particles are formed by unnecessary components such as fatty acids, Separation is not smooth and the extraction amount of the active active ingredient becomes low, which is not efficient.

The alcohols may be aliphatic or aromatic alcohols commonly used in the art, and preferably lower alcohols having 1 to 6 carbon atoms are preferably used than aliphatic alcohols.

The separated extract is concentrated under reduced pressure at 50 to 90 ° C to remove residual solvent. The recovered alcohol is added to the concentrate obtained by concentrating the filtrate under reduced pressure, and then centrifuged at 700 to 1,200 rpm to give a concentrate. And then concentrated again under reduced pressure.

The concentrate thus obtained is vacuum dried at 0.08 to 0.3 pa at 50 to 90 ° C., and then pulverized and sterilized to 30 to 200 mesh to obtain a powdery chilbladder extract. Such chilbladder extract has an excellent therapeutic action such as arthritis, Herbal medicines containing this extract are expected to be useful as joint protectors.

Chilbap extract according to the present invention is formulated in a conventional manufacturing method for the manufacture of tablets, capsules, injections, etc. Among them, lactose, microcrystalline cellulose, magnesium stearate, and the like, which are used as a base for preparing tablets, are combined When the extract is used in a ratio of 1: 1, a tablet having an activity for treating diseases caused by inflammatory changes such as arthritis can be prepared.

In the preparation of such medicaments, it may be used as a crude drug extract itself, but may be prepared by mixing with a pharmaceutically acceptable carrier, a forming agent, a dilute, etc., and preparing it as a powder, granule, Is possible. In addition, chile chile extract according to the present invention has been used for food and medicinal from ancient times, there is no particular restriction on the dosage, body absorption, weight, age, sex, health status, diet, time of administration, administration It may vary depending on the method, the rate of excretion, the severity of the disease and the like. In general, chile yeast extract is preferably administered in about 0.1 to 10 mg per 1kg body weight.

Therefore, the composition containing the active ingredient of the present invention is to be prepared in consideration of the effective amount range, and the unit dosage form formulated in this way according to the judgment of the expert and the needs of the individual to monitor or observe the administration of the drug as needed Specialized medications can be used or administered at regular intervals.

According to another aspect of the present invention, the present invention provides a food for preventing and treating inflammatory diseases, in particular a health functional food, containing chile chile extract as an active ingredient.

In the present invention, "health functional food" refers to a food having a bioregulatory function such as prevention and treatment of diseases, biological defense, immunity, recovery from illness, and aging inhibition, and should be harmless to the human body when taken in the long term.

In order to be used for the prevention and treatment of inflammatory diseases of the present invention, the chilbap extract of the present invention may be prepared by a variety of methods known in the food science or pharmaceutical field, and by itself or a food acceptable carrier, excipient, diluent It can be prepared in any food form that can be ingested orally by mixing with the back. Preferably in the form of beverage, ring, granule, tablet or capsule.

The health functional food of the present invention may be added conventionally in the production of food and may further include foodstuff acceptable. For example, in the case of beverage preparation, one or more components may be further included in citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, juice, etc. in addition to the plant extract of the present invention.

The amount that can be included as an active ingredient of the health functional food according to the present invention can be appropriately selected according to the age, sex, weight, condition, and symptom of a person who desires to prevent or treat an inflammatory disease, 0.01 g to 10.0 g, and an anti-inflammatory effect can be obtained by ingesting a health functional food having such a content.

Hereinafter, the present invention will be described in more detail with reference to Examples. These embodiments are only for illustrating the present invention, and thus the scope of the present invention is not construed as being limited by these embodiments.

Example  : Chirping Wall  Preparation of extract

After washing with water and drying well, Chilbap was extracted twice with hot water every 2 hours while adding 30% ethanol aqueous solution and stirring well. The extract was cooled slowly to room temperature and centrifugal filtration was performed to remove the residue. The filtrate was combined and concentrated under reduced pressure at 50 to 90 ° C.

Ethanol recovered through the ethanol fraction was added to the concentrate, and the concentrate was suspended sufficiently. The filtrate was then centrifuged at 5000 rpm. The filtrate was concentrated under reduced pressure, and then vacuum dried at 60 ° C under 0.08 Pa. Followed by pulverization. The content of actinigen in the chile chile extract extracted and purified by the above method was 8.25% by weight. In addition, the chile chile extract obtained by the above method was analyzed by HPLC under the following conditions.

1) Solvent:

 99.5: 0.5 (Methanol 100%: Acetic acid)

2) Column: C18 reverse phase (Agilent Eclipse XDB-C18 / 1.75 [mu] m, 2.1 * 50 mm)

3) Flow rate: 0.6 ml / min

4) Column temperature: 20 ℃

5) Detector: UV 260 nm

Experimental Example  One : TPA  Judo Edema  Suppression test ( TPA - induced mouse ear edema .)

TPA-induced ear edema inhibition test (TPA-induced mouse ear edema.) Was performed on the extract of chile biliary biloba prepared by the above example, and the results are shown in Table 1 and FIG. 2.

[Experimental Method 1]

Seven-week-old ICR mice were isolated from each experimental group, and after 1 hour after oral administration of 150 mg / Kg of Aspirin and 100, 200, 400 mg / Kg of Chilbladder extract, TPA (2.5㎍ / 20µl) After dissolving in acetone, it caused edema in the ears of mice. When inducing edema, the experimenter was completely fixed from behind and the second experimenter stimulated edema-causing substances in the ear using a micropipette. Four hours later, ear edema was measured in each experimental group. At the time of measurement, the tibial excavation method was used to precisely measure the specimen.

process Ear thickness Degree of inflammation inhibition (%) Non-treatment 0.43 + 0.026 0 % Aspirin (150 mg / kg) 0.32 ± 0.003 25.58% Chilbale Dam Extract (100 mg / kg) 0.30 ± 0.03 30.23% Chilbale Dam Extract (200 mg / kg) 0.28 ± 0.01 35.71% Chilbale Dam Extract (400 mg / kg) 0.30 ± 0.01 30.23% 1. Data represent the mean of difference in ear thickness (mm) ± SEM (n = 12)
2. Non-treatment: After the induction of edema,

As shown in Table 1, the TPA-induced ear edema inhibitory effect is excellent when the chilbale extract prepared in accordance with the present invention is used. It can be seen.

Experimental Example 2: arachidonic acid (Arachidonic acid ) induced ear edema inhibition test (Arachidonic acid-induced mouse ear edema assay)

Arachidonic acid-induced mouse ear edema assay was performed on the chile chile extract prepared by the above example, and the results are shown in Table 2 and FIG. 3.

[Experimental Method 2]

Seven-week-old ICR mice were isolated for each experimental group, and then 1 hour after oral administration of 150 mg / Kg of aspirin and 100, 200, 400 mg / Kg of Chilbale extract, and arachidonic acid dissolved in acetone (2 mg / 20 μl) Subject edema in the ear. When inducing edema, the experimenter fixed the specimen completely from the back, and then the second experimenter stimulated the edematous substance in the ear using a micropipette. One hour later, ear edema was measured for each natural product. At the time of measurement, the tibial excavation method was used to precisely measure the specimen.

process Ear thickness Degree of inflammation inhibition (%) Non-treatment 0.47 ± 0.01 0 % Aspirin (150 mg / kg) 0.39 + - 0.05 17.02% Chilbale Dam Extract (100 mg / kg) 0.34 ± 0.01 27.66% Chilbale Dam Extract (200 mg / kg) 0.30 ± 0.01 36.17% Chilbale Dam Extract (400 mg / kg) 0.30 ± 0.01 36.17% 1. Data represent the mean of difference in ear thickness (mm) ± SEM (n = 12)
2. Non-treatment: After the induction of edema,

As shown in Table 2, when the chile chile extract prepared by the present invention was used, the arachidonic acid-induced ear edema suppression effect was excellent. It can be seen that it is preferable.

Experimental Example 3: Acetic acid induced stretching inhibition test ( Acetic acid - induced writhing response in mice.)

The acetic acid-induced stretching inhibitory test (Acetic acid-induced writhing response in mice) was performed on the chile chile extract prepared by the above example, and the results are shown in Table 3 below.

[Experimental Method 3]

In this experiment, the 7-week-old ICR mice were isolated for each herbal candidate by referring to the "Siegmund" test method, and then 1 hour after oral administration of 150 mg / Kg of aspirin and 100, 200, and 400 mg / Kg of chile biliary extract. 0.075%) of intraperitoneal administration (0.1 ml / 10 g) caused inflammation of the organs. After 10 minutes, we observed the mouse's streching for 10 minutes because it causes itching at the time of edema, and the degree of pain can be estimated through this phenomenon.

process Torsion frequency
(Writhing number) Pain reduction (%) Non-treatment 17.00 ± 0.00 0 % Aspirin (150 mg / kg) 13.00 ± 0.00 23.53% Chilbale Dam Extract (100 mg / kg) 6.67 ± 0.91 60.78% Chilbale Dam Extract (200 mg / kg) 6.33 ± 0.47 62.75% Chilbale Dam Extract (400 mg / kg) 7.50 ± 0.92 55.88% 1. Data represent the mean of difference in ear thickness (mm) ± SEM (n = 12)
2. No treatment: After treatment with stretching,

As shown in Table 3, it is found that the acetic acid-induced stretching inhibitory effect is excellent in the case of the use of the chile chile extract prepared according to the present invention, and particularly, the concentration of the acetic acid-induced stretching in the case of 200 mg / kg concentration of the chile chile extract extract is most preferable. Can be.

Experimental Example  4: Acute Arthritis Treatment Effect Test

The acute arthritis treatment effect assay was performed on the chile chile extract prepared by the above example, and the results are shown in the following Table 4 and Figure 5.

[Experimental Method 4]

Male SD rats (approx. 200 g) were treated with oral aspirin 150 mg / Kg, gallbladder extract 100, 200, and 400 mg / Kg in 1 ml, and 100 μl of 1% carrageenan saline was injected into the left foot. Edema was induced. Edema was measured using a plethysmometer (LE 7500) after 4 hours.

process Edema growth rate (%) The degree of suppression of edema (%) Non-treatment 89.36 0 Aspirin (150 mg / kg) 27.04 69.74 Chilbale Dam Extract (100 mg / kg) 50.55 43.43 Chilbale Dam Extract (200 mg / kg) 79.95 10.53 Chilbale Dam Extract (400 mg / kg) 41.60 53.45 1. Data represent the mean of difference in ear thickness (mm) ± SEM (n = 12)
2. No treatment: After treatment with stretching,

As can be seen from Table 4, when the chilbladder extract prepared according to the present invention is used, the acute arthritis therapeutic effect is excellent, and it can be seen that the acute arthritis therapeutic effect is most preferable when the chilbladder extract 400 mg / kg concentration is used. .

Experimental Example  5: NO  Effect on

The acute arthritis treatment effect assay was performed on the chile chile extract prepared by the above example.

[Experimental Method 5]

After 24 hours of treatment with LPS (1 / ml) in the raw 264.7 cell line, a mouse-derived macrophage, nitric oxide, an inflammation-inducing molecule in the body, was collected by enzymatic and radical chromatography. The effect of Chilgapdam extract on the production of NO) was investigated. For measurement of NO, 50 μl of the culture solution was taken, and 25 μl of Greases solution 1 and 2 were mixed to measure absorbance at 540 nm. Absorbance values were measured using an ELISA kit (R & D systems, USA).

Chirping Wall
extract
(占 퐂 / ml) 0 0.5 5 50 500 Result contents Absorbance value Inhibition rate
(%) Absorbance value Inhibition rate
(%) Absorbance value Inhibition rate
(%) Absorbance value Inhibition rate
(%) Absorbance value Inhibition rate
(%) shame 0.228 ± 0.023 0 0.182 ± 0.026 9.44 0.156 ± 0.013 22.35 0.179 ± 0.122 10.93 0.157 ± 0.033 22.19

After treatment with LPS (1 / ml) and Chilbap extract (0, 0.5, 5, 50, 500 ㎍ / ml) on the Raw 264.7 cell line, a mouse-derived macrophage, the culture solution was collected after 24 hours, and the enzyme method was used in the body. As a result of quantifying NO, an inflammation-inducing molecule, NO production of macrophages induced by LPS was effectively inhibited in cell lines treated with Chilbap extract.

Manufacturing example  1: Preparation of tablets

Tablets for oral administration were prepared using the wet granules method and the dry granules method with the following composition using the chile chile extract prepared according to the embodiment of the present invention.

[Furtherance]

Chilbale extract 200 mg, hard silicic acid anhydrous 10 mg, magnesium stearate 2 mg, microcrystalline cellulose 50 mg, starch glycolate 25 mg, lactose 101 mg, povidone 12 mg, ethanol anhydride.

Manufacturing example  2: Preparation of Ointment

The ointment was prepared by the following composition using the chile yeopdam extract prepared according to the embodiment of the present invention.

[Furtherance]

Horse chestnut extract 5 g, cetyl palmitate 20 g, cetanol 40 g, stearyl alcohol 40 g, myristan isopropyl 80 g, monostearic acid sorbitan 20 g, polysorbate 60 g, paraoxybenzoic acid profile 1 g, 1 g of methyl paraoxybenzoate, phosphoric acid and purified water.

Manufacturing example  3: Preparation of Injection

Injections were prepared by the following composition using the chile yeopdam extract prepared according to the embodiment of the present invention.

[Furtherance]

Horse chestnut extract 100 mg, mannitol 180 mg, sodium hydrogen phosphate 25 mg, purified water for injection 2974 mg

Manufacturing example  4 : Transdermal  Produce

A percutaneous agent was prepared by using the Chil-yop-dam extract prepared according to an embodiment of the present invention with the following composition.

[Furtherance]

Horse chestnut extract 0.4 g, sodium polyacrylate 1.3 g, glycerin 3.6 g, aluminum hydroxide 0.004 g, methyl paraben 0.2 g, water 14 g.

Manufacturing example  5: preparation of beverages

The beverage was prepared as follows using the chile yeopdam extract prepared by the embodiment of the present invention. Chilgapdam extract 200mg, vitamin C 75 mg, vitamin B2 4 mg, vitamin B6 3 mg, dietary fiber 1,000 mg, liquid fructose 20000 mg, emulsifier 100mg, fragrance 50mg is mixed to 50 ml of the volume. The final mixture was clarified by passing the mixed solution through a filter of 2 ~ 3㎛ before sterilization at 90 ~ 93 ℃ for 15 ~ 20 seconds, filled into 50ml bottle and sterilized after 15 ~ 20 minutes at 80 ~ 85 ℃. Completed.

Claims (5)

delete delete Extracting, cooling, and filtering one from a crowd consisting of water, alcohol, and an aqueous alcohol solution of 5 to 8 times the weight of the crude medicine of Gynostemma pentaphyllum (七葉 膽, Gynostemma pentaphyllum),
Separating the filtrate obtained above into an alcohol aqueous solution and concentrating it under reduced pressure at 50 to 90 ° C, and
Concentrating the filtrate was centrifuged at 700 ~ 1,200 rpm after the addition of alcohol to the concentrate obtained in the concentrate under reduced pressure to prepare an extract comprising a 5.0 to 10.0% by weight of the actinogen (Arctigenin) as an indicator material Method for preparing bile extracts. delete delete

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