KR101336626B1 - DHA Egg Produced With Schizochytrium sp. - Google Patents

Abstract

The present invention is a DHA egg produced using Shijokaitrium. This means that 99.70 to 99.85% by weight of poultry feed and Schizochytrium sp.) Feed mixed with dry powder at a ratio of 0.15 to 0.30% by weight (moisture content less than 5% by weight relative to the total weight of Sijokaitrium), fed 90 to 110 g daily to 140 to 180 days old chickens after hatching. , Eggs laid after 3 weeks after the start of feeding, DHA content based on the total fat content of the egg is 3 to 4% by weight, EPA content is 0 to 0.1% by weight, Omega- with respect to the content of omega-3 fatty acids 6 The content of fatty acids may be 2 to 4 times. Accordingly, not only commercially economical but also have significant effects such as maximizing the efficacy of DHA when ingested by children or infants.

Description

DHA Egg Produced with Shijo Kaitrium {DHA Egg Produced With Schizochytrium sp.}

The present invention generally relates to DHA eggs containing DHA produced using Schizochytrium sp., A microalgae containing a large amount of omega-3 fatty acid docosahexaenoic acid (DHA). In more detail, the eggs are produced by chickens fed a feed containing Sizokaitrium, wherein the produced eggs contain more than a certain ratio of DHA to total fat content and EPA (Eicosapentaenoic acid) content. Is minimized and also optimizes the content of omega-6 fatty acids to omega-3 fatty acids, thereby allowing DHA eggs to be produced using the new Shijokaitrium, which maximizes the efficacy of DHA when ingested.

EPA, which is generally regarded as the representative of omega-3 fatty acids, is known to be effective in cardiovascular and immune function, and DHA is effective in brain and optic nerve function. In particular, DHA is an essential fatty acid for nervous and ocular tissues, which is an important factor in developing vision and motor skills of infants. Most of them are extracted from blue fish such as mackerel and saury, but they are used industrially. There are many problems to use as a raw material.

Also, in general, ingested fish always contain EPA and DHA simultaneously, so it is impossible to consume DHA alone through fish. In particular, the intake of DHA in infants, because the intake of EPA at the same time may cause side effects due to thrombolysis and immune function is a function of EPA. Therefore, there is a need for a method of selectively ingesting DHA.

In general, the method of adding DHA to eggs is to add fish oil to the feed, and to add the raw material (perilla oil, etc.) containing a large amount of linolenic acid in the vegetable oil to the feed. However, the egg produced by feeding fish oil has a low fish power due to the fish's distinctive fishy odor is transferred to the egg, and EPA accumulates simultaneously, making it impossible to selectively consume only DHA.

In general, the production method of eggs containing polyunsaturated fatty acids (omega fatty acids) is added to feed the raw material (perilla oil) containing a large amount of linolenic acid in vegetable oil to feed the feed to contain the final metabolite DHA will be. When an animal absorbs linolenic acid (18: 3n-3), it is found that 18: 4n-3 20: 4n-3 20: 5n-3 (EPA) 22: 5n-3 22: 6n-3 (DHA) The steps lead to the accumulation of the final DHA. Therefore, intermediate metabolites accumulate in the body, and the amount of DHA, the final product, is very small. In addition, a large amount of linolenic acid (18: 3n-3) must be fed to contain 1% or more of DHA in the total fat content of eggs. In this case, the profitability is not met due to the increase in feed prices, which makes no business feasibility. Therefore, vegetable oils containing linolenic acid (18: 3n-3) must be fed at a cost-free range, and the accumulation of DHA in eggs is extremely small.

Another method for producing eggs containing polyunsaturated fatty acids (omega fatty acids) is to feed fish by mixing fish oil extracted from fish. In eggs produced in this way, fish oil derived from fish oil is transferred to the eggs and degrades. In addition, EPA and DHA are included at the same time, there is a problem that it is impossible to selectively receive only the DHA that is absolutely necessary for infants and children.

In addition, the method of adding vegetable oil (eg, perilla oil) containing a large amount of linolenic acid to feed is extremely low in DHA accumulated in eggs, and is unsuitable commercially due to the price increase using vegetable oil. In addition, since rancidity occurs when fish or vegetable oil is added directly to the feed, a method for preventing oxidation is required.

Korean Patent Publication No. 10-2010-0097568 conventional founders in number and hangukga Journal Friday the 35, No. 3 (July 2008.9) Kite Leeum (Schizochytrium There is disclosed a technique for fermenting soybean meal using sp.) and then mixing the fermented soybean meal with feed. However, Schizochytrium is a microalgae used for fermentation of powder such as soybean meal. It is almost impossible to ferment sp. Therefore, this method is based on the Schizochytrium contained in fermented soybean meal. The content of DHA also changes according to the amount of sp.), so that the amount of DHA contained in chickens fed this feed is not constant. In addition, the technology adds a process for fermenting soybean meal, which adds a large powder fermentation facility, and the input amount is pure Schizochytrium. It is a commercial disadvantage because it is more than 10 times compared to sp. dry powder.

Therefore, in the production technology of eggs containing DHA, not only the content of DHA is relatively high, but also the content of omega-3 fatty acids and omega-6 fatty acids can be lowered to the minimum, and the content of omega fatty acids can be used. There is still a need for a technology that allows for maximum maximization and commercially low cost production.

The present invention has been made to solve the above-mentioned problems of the prior art and to add various other advantages, so that the eggs are produced by the feed chickens containing Schizochytrium sp. To ensure that the eggs contain at least 3% by weight of DHA relative to the total fat content, the EPA content is less than 1% by weight, and the ratio of omega-6 fatty acids to omega-3 fatty acids is optimized to 1: 3. It is therefore an object of the present invention to provide DHA eggs produced using the new Shijokaitrium, which are not only commercially economical but also allow the infant to maximize the efficacy of DHA.

This object is achieved by a DHA egg produced using Shijochitrium provided according to the present invention.

DHA eggs produced by using the Sizokaitrium provided according to an aspect of the present invention, 99.70 to 99.85% by weight of poultry feed and 0.15 to 0.30% by weight of dry powder of Sijokaitrium (moisture content relative to the total weight of Sijokaitrium Silver is fed at a ratio of less than 5% by weight), 90 to 110g daily feed to chickens 140 to 180 days after hatching, and eggs laid three weeks after starting feeding, the total fat content of the eggs The reference DHA content is 3% by weight or more, the EPA content is 0.1% by weight or less, and the content of omega-6 fatty acids may be 2-4 times the content of omega-3 fatty acids.

Here, the dry sijochitrium powder, after cultivating a large amount of Schizochytrium sp. In a fermenter, the wet weight is concentrated to 40% by weight relative to the total amount of Schizochytrium sp. It may be to dry by hot air spray drying method at 160 ℃ and have a particle size of less than 100 mesh.

According to the present invention having the above-described configuration, eggs are produced by chickens fed a feed containing Sizokaitrium, but the DHA content of the total fat content in the produced eggs contains, for example, 3% by weight or more, and EPA. Content is minimized to less than 0.1% by weight and the ratio of omega-6 fatty acids to omega-3 fatty acids is optimized to 1: 3, which is not only commercially economical but also maximizes the efficacy of DHA when consumed by children and infants. There is a remarkable effect such as to make.

1 is a schematic flowchart illustrating a process of producing a DHA egg produced by using the sijochitrium according to an embodiment of the present invention.

Advantages and features of the present invention and methods for achieving them will be apparent with reference to the embodiments described below in detail with the accompanying drawings. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art. To fully disclose the scope of the invention to those skilled in the art, and is defined by the claims of the present invention.

In the following description of the present invention, detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear. The following terms are defined in consideration of the functions in the embodiments of the present invention, which may vary depending on the intention of the user, the intention or the custom of the operator. Therefore, the definition should be based on the contents throughout this specification.

Hereinafter, with reference to the drawings will be described DHA eggs produced using the Shijo chitrium according to a specific embodiment of the present invention.

DHA eggs produced using the Schizochytrium sp. Provided in accordance with an aspect of the present invention, as illustrated in FIG. 1, are generally cultured in a commercially available poultry feed (11). It relates to a feed (14) of the mixed powder 13 of the dry powder (12) of Shijo Kaitrium to the chicken (14), and to the eggs (15) laid after a predetermined period of time after starting feeding.

The DHA egg of the present invention thus produced has a DHA content of 3 to 4% by weight and an EPA content of 0 to 0.1% by weight based on the total fat content of the egg, and a content of omega-6 fatty acid with respect to the content of omega-3 fatty acid. It may be 2 to 4 times, preferably 3 times.

As such, the process of producing the DHA eggs of the present invention may be divided into preparing a feed and feeding the feed to chickens.

In the preparation of the feed, poultry feed 99.70 ~ 99.85% by weight and Schizochytrium sp.) The feed may be prepared by mixing dry powder at a ratio of 0.15 to 0.30% by weight (moisture content less than 5% by weight relative to the total weight of schizotrium). Here, the poultry feed generally refers to poultry feed commercially available. There are various types of poultry feeds available for sale, for example, the hens star-100 can be used as a product manufactured in dominant feed, but the poultry feed used in the present invention is not limited thereto. Both conventional poultry feed and various poultry feeds that can be developed in the future can be used.

Sizochitrium is particularly well known as a marine microalgae containing high amounts of DHA. Micro algea is a single cell photosynthetic organism that lives in freshwater and seawater and is representative of chlorella, spirulina, and sizochitrium. They are known to produce 5 to 10 times more biomass than land plants, especially because of their rapid growth, and are capable of mass cultivation in a highly commercial and inexpensive manner.

Here, Shijo Kaitrium dry powder is, after mass culture of Shijokaitrium in a fermenter, once the wet weight is concentrated to 40% by weight relative to the total amount of Shijokaitrium with a concentrator, and then at 140 to 160 ℃, preferably about 150 Drying by hot air spray drying at ℃ may be to have a particle size of less than 100 mesh.

The feed prepared above is then fed to the chickens 140-180 days old after hatching, preferably to 160-day-old chicken in an amount of 90 to 110g, preferably about 100g daily. Eggs produced from about three weeks after the start of feeding of the mixed feed of Sijokaitrium are suitable as DHA eggs according to the present invention.

In other words, the DHA egg according to the present invention has a DHA content of 3 to 4% by weight and an EPA content of 0 to 0.1% by weight based on the total fat content of the egg, and a content of omega-6 fatty acid with respect to the content of omega-3 fatty acid. It may be 2 to 4 times, preferably about 3 times.

Afterwards, the chicken is continuously fed with a mixed feed of Sijokaitrium dry powder so that the DHA eggs according to the present invention can be continuously produced.

<Examples>

1) Estimation of Proper Mixing Ratio between Shijo Kaitrium Dry Powder and General Feed

In this example, DHA content of 3% by weight or more based on total fat content in eggs was considered in consideration of commercial and proper DHA content, and the DHA content in eggs was measured after feeding for 3 weeks in chickens of 160 days of age. Table 1 is as follows.

division Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Shijo Kaitrium dry powder 0.00 0.05 0.10 0.15 0.20 0.25 0.30 n-3 fatty acid 0.5 1.2 2.3 3.2 3.7 3.8 3.9 n-6 fatty acid 11.9 11.9 11.6 11.4 11.2 11.2 11.1 DHA 0.2 1.1 2.1 3.0 3.5 3.7 3.8

* Daily feed: 100g per poultry

* Shijo Kaitrium dry powder: Less than 5% by weight of moisture

* Shijo Kaitrium dry powder content: weight% of the total weight of the feed

* n-3 fatty acid, n-6 fatty acid, DHA content:% by weight of total fat content in eggs

According to these results, DHA content in eggs increases as the content of dry Sijokaitrium powder in feed increases, but results of 3% (w / w) or more based on the total fat content in eggs are considered in consideration of commercial and appropriate levels of DHA. Sagichitrium dry powder content was found to be 0.15 ~ 0.30% by weight. In Example 5, which is a preferred embodiment of Table 1, EPA is fed a feed prepared by mixing 99.8 g of commercially available poultry feed with 0.2 g of dried Sijokaitrium powder in 100 g of daily feed of poultry. Almost no, only DHA was selectively contained, and the ratio of omega-3 fatty acids and omega-6 fatty acids was 1: 3, thereby producing eggs that maximize the efficacy of omega fatty acids.

2) Accumulation of DHA by Feeding Period

In the case of Example 5, which is a preferred embodiment of the present invention in Table 1 above, DHA content in eggs by feeding period satisfying the DHA content of 3% by weight or more based on the total fat content in eggs in consideration of commerciality and proper DHA content (egg The weight of the total fat content in the weight percent) is measured as shown in Table 2 below.

division 1 week 2 weeks 3 weeks 4 weeks 5 weeks 6 weeks General Feed Feed Eggs 0.2 0.2 0.2 0.2 0.2 0.2 Feed Feed Experiment 0.5 1.9 3.5 3.9 4.7 5.9

* General feed: poultry feed that is commercially available on the market

* Experimental feed: feed mixed with 99.8 g of general feed + 0.2 g of Sijokaitrium dry powder according to the present invention

* Daily feed: 100g / 1 poultry

As a result, the DHA content in the dietary feed eggs hardly changes according to the feeding period, but the experimental feed feeding eggs according to the present invention are eggs required by the present invention after feeding for 3 weeks. DHA content of more than 3% by weight based on the total fat content satisfied. Although not shown in the table, EPA was almost as 0 ~ 0.1% by weight. Accordingly, it can be seen that eggs containing only DHA selectively and the ratio of omega-3 fatty acids and omega-6 fatty acids are 1: 3 can be produced to maximize the efficacy of omega fatty acids.

As described above, according to the present invention, the eggs are produced by chickens fed a feed containing Schizochytrium sp., But the amount of DHA to the total fat content of the produced eggs is, for example, 3% by weight. EPA content is minimized to less than 0.1% by weight and the ratio of omega-6 fatty acid to omega-3 fatty acid is optimized to about 1: 3, which is not only commercially economical but also effective for the consumption of infants and toddlers. By maximizing, it does not contain EPA having a geriatric disease prevention effect function which is not necessary in children and infants, and has a remarkable effect such as selectively containing only DHA having an effect on brain function and optic nerve.

Claims (2)

delete Feed for chickens of 99.70 to 99.85% by weight and dry powder of Schizochytrium sp. 0.15 to 0.30% by weight is fed 90-110 g daily to 140-180-day-old chickens after hatching. Eggs spawned after 3 weeks from the start, the DHA content based on the total fat content of the egg is more than 3% by weight, the EPA content is less than 0.1% by weight, the content of omega-6 fatty acid relative to the content of omega-3 fatty acid Is 2-4 times
The Shijo Kaitrium dry powder, after culturing a large amount of Shijo Kaitrium in a fermenter, the wet weight is concentrated to 40% by weight relative to the total amount of Shijokaitrium with a concentrator, dried by hot air spray drying at 140 ~ 160 ℃ DHA eggs produced using Sijokaitrium, characterized in that the water content is less than 5% by weight relative to the total weight of the chitrium and has a particle size of 100 mesh or less.

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