KR101314557B1 - Method for treating or preventing viral diseases of domestic animals - Google Patents

Abstract

The present invention relates to a method for treating or preventing a viral disease such as foot-and-mouth disease, avian influenza, and more specifically, a first step of feeding a feed containing an organic acid to a livestock and a feed containing a stomach organic acid staying in the digestive organs of the livestock. By feeding the drinking water in which sodium chlorite was dissolved to produce chlorine dioxide with a relatively constant concentration in the digestive organs of the livestock, thereby allowing the serotype to kill the virus by allowing nonspecific reaction with various viruses. The present invention relates to a method for treating or preventing viral diseases such as foot and mouth disease, bird flu, which can minimize the economic damage of livestock farmers by suppressing the spread of viruses such as viral diseases such as foot and mouth disease and bird flu.

Description

Methods for treating or preventing viral diseases of domestic animals}

The present invention relates to a method for treating or preventing viral diseases such as foot-and-mouth disease such as cattle and swine, and bird flu such as chicken.

Viral disease in livestock is highly contagious and therapies are not effective, so it is not easy to respond effectively. Virus diseases of domestic animals include foot and mouth disease of cows and pigs, and bird flu such as chickens and ducks.

Bird flu or avian influenza (bird flu, AI) is an infectious respiratory disease that affects birds. All avian influenza known to date belong to one species, influenza A. Influenza is a problem because it can adapt across species and can infect people in particular.

Foot and mouth disease (FMD) is a highly contagious disease that infects hoofed animals such as cows, pigs, sheep, goats, or deer (cows), and is a worldwide disease, including Africa, Asia, the Middle East, and South America. Onset.

According to the recent Kitching et al., Foot-and-mouth disease virus, which is currently prevalent in Asia, belongs to the South Asia topotype of serotype O, which occurred in Korea in 2000, 2002 and 2010, causing severe economic damage to livestock farms. .

In the early stages of infection, blisters develop between the lips, tongue, gums, nose or hooves, and body temperature rises rapidly, resulting in decreased appetite and eventually acute death. The foot-and-mouth disease area has a mortality rate of 55% and a transmission rate of 90%. In Korea, it is a type 1 statutory livestock epidemic and is classified as a List A disease by the OIE.

Foot-and-mouth disease virus is a single-stranded positive sense RNA virus belonging to the Picornavirnae Aphtovirus and has 8,500 base pairs.The genome expresses one protein and the virus Viral proteinases are characterized by cleavage of viral proteins into mature proteins. The viral envelope is composed of VP1, VP2, VP3, VP4 proteins, and nonstructural proteins involved in viral replication and protein maturation include L, 3A, 3B, 3C, 3D, 2A, 2B, and 2C.

Enveloped proteins (Capsid proteins or SPs) induce antibodies but do not produce cross-defense against other virus types due to the different characteristics of the antibodies produced depending on the viral serotype, and non-structural proteins (NSPs) are most effective for viral replication and infection. It is a protein that plays an important role and does not induce antibodies, but is known to be cross reactive against seven serotype viruses. This is due to the higher identity of the nonstructural proteins than the envelope proteins surrounding the viral envelope, A. Calvijo et al.

In order to control foot-and-mouth disease, non-foot-and-mouth disease-free areas and clean countries implement non-vaccine policies, emergency vaccine policy and killing policy when foot-and-mouth disease occurs, and foot-and-mouth disease countries are prevented by inoculating foot-and-mouth vaccine. The foot-and-mouth vaccine has no cross-defense between seven serotypes (O, A, Asia, C, SAT1, SAT2, and SAT3 serotypes). Concentrated dead venom vaccine after purification in supernatant is used. However, due to the development of transportation, increased import and export trade, illegal smuggling, etc., as in the United Kingdom, the possibility of outbreak is increasing due to unforeseen virus types, and environmental problems caused by incineration and burial are rising. have.

Bird flu is also known as Bird Flu, also known as bird flu or poultry influenza. Viral diseases occur in poultry such as chickens and ducks. Birds that come in contact with a runny nose, respiratory secretions, or feces of an infected bird are transmitted again in a form that is infected again, especially by migratory birds.

Avian influenza is classified into highly pathogenic, medicinal and non-pathogenic according to pathogenicity. Highly pathogenic avian influenza is classified as the first type of infectious animal infectious disease in Korea. Chickens are particularly susceptible and more than 80% of them die from dyspnea if infected.

Avian influenza viruses have HA and NA types. 15 HA type and 9 NA type are multiplied, and theoretically 135 viruses exist. Types H5 and H7, which cause avian influenza in birds, do not, in principle, infect humans, but rarely, like H5N1 influenza, in humans.

The present invention provides a method for treating or preventing viral diseases such as foot-and-mouth disease, poultry flu and the like simply by providing food to livestock.

The present invention provides a method for treating or preventing viral diseases such as foot-and-mouth disease, bird flu, which can be supplied in large quantities and has no objection to livestock.

The present invention provides a first step of feeding the feed containing the organic acid to the livestock and a second step of generating chlorine dioxide in the digestive system of the livestock by feeding the chlorine dissolved drinking water while the feed containing the organic acid stays in the digestive organs of the livestock Provided are methods for treating or preventing livestock viral diseases.

The present invention provides a method for the treatment or prevention of livestock viral diseases comprising a stomach organic acid in an amount capable of maintaining the pH of drinking water in which stomach chlorite is dissolved at 6-7.

The present invention enables the effective treatment of viral diseases of cattle directly in farms in a manner that provides food to the cattle.

The treatment or prevention method of the present invention enables an effective response in the event of a large outbreak of a sudden viral disease of a domestic animal.

The therapeutic or prophylactic methods of the present invention can be applied regardless of the type of virus since they are not based on specific responses such as antigens, antibody responses, and the like.

Maintaining the reaction conditions of organic acid and chlorite at pH 6-7 ensures the continuous production of chlorine dioxide in concentrations and amounts suitable for the treatment of foot-and-mouth disease of normal size and bird flu treatment in chickens.

The present invention relates to a method for treating or preventing a viral disease in a livestock, and more particularly, a first step of feeding a feed containing acid to a livestock and drinking water in which chlorite is dissolved while the feed containing the acid stays in the digestive organs of the livestock. By feeding a second step to generate a chlorine dioxide in the livestock digestive organs to maintain a relatively constant concentration of serotypes to induce death through non-specific reactions with a variety of viruses, and spread the virus, such as foot-and-mouth disease, bird flu The present invention relates to a method for treating or preventing foot-and-mouth disease that can effectively suppress the economic damage of livestock farmers.

Hereinafter, the present invention will be described in more detail.

The treatment or prevention method of the present invention includes a first step of feeding a feed containing an organic acid to a livestock.

Livestock is not limited as long as it can be a viral disease, but is preferably a bird, such as cows, pigs, sheep, goats or deer, hoofed animals (chicken), chickens, ducks, etc. Chickens are particularly preferred.

The organic acid lowers the pH of drinking water in which chlorite described later is dissolved to generate chlorine dioxide gas.

The organic acid may be any organic acid capable of reacting with sodium chlorite dissolved in an aqueous solution in a livestock digester to generate chlorine dioxide, including strong and weak acids.

Hydrochloric acid, sulfuric acid, nitric acid, or the like or a mixed acid thereof may be used as a strong acid, and citric acid, acetic acid, lactic acid, or a mixed acid thereof may be used as a weak acid. If necessary, a strong acid and a weak acid may be mixed and used.

However, in consideration of the continuous generation of chlorine dioxide gas and safety and economics, acetic acid, citric acid or lactic acid, which is a weak acid, is preferable, and citric acid is more preferable.

The organic acid is preferably contained in an amount capable of lowering the pH of drinking water in which chlorite described later is dissolved, to about 6-7. Depending on the type of acid, the amount that can lower the pH to 6-7 will be slightly different.For example, 100-500 µl of drinking water with 0.1-0.5% chlorite concentration is 0.1-5mg per kg of livestock. The organic acid of may be added to lower the pH to 6-7.

The amount of the organic acid used may be determined in consideration of the generation rate, concentration, and the like of the chlorine dioxide gas. For example, if a cow weighing 250 kg has foot-and-mouth disease, the content of organic acid in the feed can be fed about 0.1-3g, preferably 0.3-1.5g, about 3-5 times a day, and the concentration of chlorite after feeding 50-200ml of 0.1-1% drinking water is fed to 250 kg cows to adjust the pH of the mixed solution to 6-7, which is close to neutral, and chlorine dioxide gas is continuously generated at a proper concentration for about 30-2 hours for a suitable time. You can do that.

Organic acids are included in livestock feed and supplied to livestock. Organic acids are not limited to specific amounts unless the livestock is sour and sour that it does not reject the feed. Preferably the livestock can be ingested organic acid by 0.1-5mg / kg per hour, for example, a cow weighing 250kg can be ingested 1.5g of organic acid every three hours.

The treatment or prevention method of the present invention includes a second step of feeding chlorine dioxide in the digestive system of the livestock by feeding the chlorine-dissolved drinking water while the feed containing the organic acid stays in the digestive system of the livestock.

Feed containing organic acids stays for a period of time in livestock digesters and reacts with chlorine-dissolved drinking water.

Chlorites (M ⁺ ClO ₂ ^-) include alkali metal chlorite, or chlorite alkaline earth metal salt that is soluble in solvents which cattle can drink. Examples of the alkali metal chlorite salt include sodium chlorite, potassium chlorite or lithium chlorite, and examples of the alkaline chlorite metal salt include calcium chlorite, magnesium chlorite or barium chlorite. Among them, sodium chlorite and potassium chlorite are preferred, and sodium chlorite is most preferred in view of dissolving in an aqueous solution so as not to rapidly generate chlorine dioxide gas and maintaining the pH at 8-9.

The use of any of these chlorites does not prevent livestock from drinking water.

Chlorite may be included in livestock drinking water in an amount sufficient to generate sufficient chlorine dioxide gas. Preferably, 100-500 μl of drinking water dissolved in 0.1-0.5% (chlorine) chlorite is dissolved in 1 hour per kilogram of livestock.For example, a cow weighing 250 kg is 0.25% (concentration) every 3 hours. 150 ml of drinking water in which chlorite is dissolved can be consumed.

[Reaction Scheme 1]

2NaClO ₂ + 2CH ₃ COOH → 2CH ₃ COONa + 2ClO ₂ + H ₂

[Reaction Scheme 2]

5NaClO ₂ + 4HCl → 4ClO ₂ + 5NaCl + 2H ₂ O

Scheme 1 shows that when acetic acid is used as organic acid and sodium chlorite is used as chlorite, sodium acetate, chlorine dioxide gas and hydrogen gas are generated in the digestive organs of livestock. Sodium chlorate, when used, indicates the generation of sodium chloride, chlorine dioxide gas and water in livestock digesters.

In the present invention, chlorine dioxide gas is generated in the digestive organs of livestock, thereby killing the foot-and-mouth disease virus that uses the livestock as a host. Since chlorine dioxide gas is not hydrolyzed, even if feed, water, and the like are mixed in the fire extinguisher, the sterilizing power is not particularly affected.

The present invention is explained in more detail by the following examples. However, the following examples are not intended to limit the scope of the present invention only to aid the understanding of the present invention.

Example  One

The effect of the treatment or prevention method of the present invention was confirmed in 40 cows (about 250 kg weight) in Gangwon-do ranch where foot and mouth disease occurred.

All 40 cows were found to have 1) body temperature above 41 ° C, 2) large amounts of blisters on nose, tongue, hooves and chest, and 3) drooling.

First, 100 g of feed containing 1.5 g of citric acid was fed to 40 cows at a weight of about 250 kg, and after about 10 minutes, 150 ml of drinking water having 0.25% sodium chlorite was ingested. This method was repeated every 3 hours for 21 days to raise cattle.

Forty cows were isolated and bred, and each night, the body temperature, number of blisters and saliva were measured, and if more than one symptom of 1) to 3) disappeared, foot and mouth disease was treated or alleviated. Judging The results are summarized in Table 1 below.

Work The number of people with foot and mouth disease Population in which foot-and-mouth disease was treated or alleviated 1-3 40 0 4-6 40 0 7-9 38 2 10-12 35 5 13-15 32 8 16-18 30 10 19-21 25 15

After 21 days, the body temperature of 15 cows whose foot and mouth disease was judged to be treated or alleviated was precisely measured by a thermal imaging camera (Testo 876, manufactured by Testo Korea). All 15 animals were found to have normal body temperature between 38.5 ° C and 39.5 ° C. It became.

Comparative Example  One

1.5 g of citric acid was mixed with 150 ml of drinking water having a concentration of 0.25% sodium chlorite for 10 cows on a 250 kg basis in the same pasture as in Example 1, and consumed for each of the 10 cows.

Eight out of ten cows did not eat at all, whether it was a bad smell or sour taste, and the other two had eaten a little, but the symptoms of 1) to 3) of the above example still persisted whether chlorine dioxide gas was blown out in the air. Confirmed.

Example  2 and Comparative Example  2

For two groups (groups A and B) containing 300 chickens infected with bird flu, group A chickens contained 250 μl of drinking water and 5 mg of acetic acid dissolved in 0.3% potassium chlorite per hour. Was fed for 21 days and chickens in group B were fed with normal feed and drinking water for 21 days.

Whether the chicken was infected with bird flu was determined by the chicken body temperature measured by a thermal imaging camera (Testo 876, manufactured by Testo Korea), considering that the chicken's normal body temperature was 41.5-41.7 ° C. When higher than 1 ℃ was considered to be infected with bird flu. Chickens infected with bird flu were immediately discarded.

Table 21 summarizes the number of deaths of chickens belonging to two groups for 21 days.

Work Cumulative number of dead chickens in group A
(Example 2) Cumulative number of dead chickens in group B
(Comparative Example 2) 1-3 4 5 4-6 6 15 7-9 10 32 10-12 12 51 13-15 14 84 16-18 14 115 19-21 14 152

As shown in Table 2, in Example 2 according to the present invention, only 14 of 300 chickens died after 21 days, whereas in Comparative Example 2, mortality exceeded 50% and the rate of bird flu transmission was very high. It was confirmed that it was fast.

Claims (8)

A first step of feeding the animal feed containing the organic acid; And a second step of feeding chlorine dioxide in the digestive system of the livestock by feeding drinking water in which chlorite is dissolved while the feed containing the organic acid stays in the digestive system of the livestock.
The method of claim 1, wherein the organic acid is contained in an amount capable of maintaining the pH of the drinking water in which the chlorite is dissolved at 6-7.
The method of claim 1, wherein the organic acid is citric acid, acetic acid or a mixture thereof.
The method of claim 3, wherein the chlorite is sodium chlorite, potassium chlorite or a mixture thereof.
The method of claim 1, wherein the organic acid is included in the feed in an amount that can be ingested by the animal at 0.1-5 mg / kg per hour.
The method of claim 1, wherein the livestock is cattle, pigs, chickens, or ducks.
The method of claim 1, wherein the chlorite-dissolved drinking water is 100-500 μl of water having a chlorite concentration of 0.1-0.5% at 1 hour per kilogram of livestock weight.
The method of claim 1, wherein the digestive organ is a stomach or small intestine.