KR101309054B1 - Preparation method Red Ginseng Extract by Low Temperature Process - Google Patents

Abstract

The present invention relates to a method for preparing a red ginseng concentrate using a low temperature method, and more specifically, extracting red ginseng by adding to an extraction solvent; Cooling the obtained extract and performing cold filtration; Low temperature concentration of the obtained filtrate; And it relates to a method for producing a red ginseng concentrate comprising the step of low-temperature aging the obtained concentrate.
The red ginseng concentrate has an excellent effect of increasing immunity and improving memory and can be applied to various health foods.

Description

Preparation method of red ginseng concentrate using low temperature method {Preparation method Red Ginseng Extract by Low Temperature Process}

The present invention relates to a method for preparing a red ginseng concentrate using a low-temperature method to contain a high content of ginsenosides, amino acids, AFG and the like active ingredients in red ginseng to improve immunity and memory.

With increasing interest in health, the use of health foods, such as boosting immunity and restoring energy, is increasing. Representative health foods include red ginseng, and these red ginseng are known to enhance immune function against infectious substances in the body by activating immune cells by saponins contained therein.

Red ginseng is produced by steaming more than 6 years old ginseng among ginseng and having about 13% moisture. It features red color. Red ginseng has sedative and excitatory effects on the central nervous system and acts on the circulatory system to prevent hypertension and arteriosclerosis. At the same time, hematopoietic effect (당 作用) and blood sugar levels (血糖 値) lowers, protects the liver, acts on the endocrine system indirectly effective on sexual behavior and reproductive effects, anti-inflammatory And anti-tumor effect (抗 腫 瘍 作用), the protective effect against radiation, protects and softens the skin. In addition, the most important of the effects of red ginseng is the adaptogen effect, which increases the ability of the living body to adapt more easily by increasing the defense ability against various diseases such as lacrimal disease and various stresses from the environment. Yes, it is scientifically proven.

Although ginseng and red ginseng have a common ingredient, red ginseng is heat treated when steaming ginseng with water vapor, so chemical conversion occurs during the manufacturing process, resulting in some new active ingredients that do not exist in ginseng or dry ginseng. There are 18 common ingredients of red ginseng and ginseng, and there are 12 new ingredients in red ginseng. For example, G-Rh2, which is effective for inhibiting cancer cell proliferation, and maltol, which is related to anti-aging effect, exist only in red ginseng. do.

The red ginseng concentrate is extracted by filtration of red ginseng with water, alcohol, or a mixed solvent thereof to concentrate the soluble red ginseng as it is, and contains a high content of soluble active ingredients in red ginseng, and is commercially available in the state of the concentrated drink.

Accordingly, various methods and apparatuses for obtaining red ginseng concentrate have been proposed.

Republic of Korea Patent Publication No. 2006-0026719 is a red ginseng powder is extracted by hot water extraction at 75 ~ 85 ℃ using a mixed solvent of water / ethanol and then cooled several times. It is showing how to get.

Moreover, as an apparatus, the apparatus which obtains a red ginseng concentrate by steaming water ginseng through steam, and steaming it with a heater in an extraction bath is marketed.

However, the extraction method or apparatus uses a method of extracting hot water at a high temperature, and the extraction process is repeated several times to obtain a high content of the active ingredient in red ginseng. As the number of times of the repeated extraction increases, the consumption cost increases and it is uneconomical, and the active ingredient in the red ginseng cannot be effectively extracted because the red ginseng concentrate is obtained through hot water extraction.

Accordingly, Korean Patent Registration No. 10-1034809 proposed a method of obtaining a red ginseng concentrate having enhanced saponin content by heating with ginseng using an extract extracted from pine needles, brown root and kelp with water.

In addition, Korean Patent Publication No. 2011-0082866 discloses a method for producing red ginseng concentrate which improves the content and palatability of active ingredients such as ginsenosides and amino sugars by extracting red ginseng with charcoal filtered purified water prepared using a charcoal filtration system. Is starting.

Republic of Korea Patent Publication No. 2006-0026719 Republic of Korea Patent Registration No. 10-1034809 Republic of Korea Patent Publication No. 2011-0082866

Accordingly, an object of the present invention is to provide a method for preparing a red ginseng concentrate, which can effectively extract various effective ingredients in red ginseng.

In order to achieve the above object,

Extracting red ginseng by adding to the extraction solvent;

Cooling the obtained extract and performing cold filtration;

Low temperature concentration of the obtained filtrate; And

It provides a method for producing a red ginseng concentrate comprising the step of aging the resulting concentrate at low temperature.

The red ginseng concentrate obtained according to the present invention contains a high content of ginsenosides, amino acids, AFG (Arginine-Frictose-Glucose) and the like in red ginseng through a low temperature method.

This red ginseng concentrate can be applied to various health foods because it is excellent in increasing immunity and improving memory.

1 is a ginsenoside-related chromatogram of red ginseng concentrate obtained in Example 1 of the present invention.
2 is a ginsenoside-related chromatogram of the red ginseng concentrate obtained in Comparative Example 1 of the present invention.
3 is an amino acid related chromatogram of the red ginseng concentrate obtained in Example 1 of the present invention.
4 is an amino acid related chromatogram of the red ginseng concentrate obtained in Comparative Example 1 of the present invention.
5 is an AFG-related chromatogram of the red ginseng concentrate obtained in Example 1 of the present invention.
6 is an AFG related chromatogram of red ginseng concentrate obtained in Comparative Example 1 of the present invention.

After extracting the red ginseng concentrate according to the present invention is obtained through a low temperature method including low temperature concentration and low temperature aging.

Specifically, the red ginseng concentrate

(S1) extracting by adding red ginseng to the extraction solvent;

(S2) low temperature filtration of the obtained extract;

(S3) low temperature concentration of the filtrate obtained; And

(S4) The obtained concentrated solution is prepared through low temperature aging.

Hereinafter, the present invention will be described in more detail at each step.

(S1) extraction process

First, an extraction process of extracting the active ingredient in red ginseng is performed by adding red ginseng to the extraction solvent.

The red ginseng used to obtain the red ginseng concentrate uses red ginseng powder or dried red ginseng, and red ginseng powder to obtain high extraction efficiency.

At this time, the extraction solvent is extracted using a solvent selected from the group consisting of water, lower glycols having 1 to 6 carbon atoms, lower alcohols having 1 to 4 carbon atoms, and mixtures thereof. Most preferably, water or alcohol is used as the extraction solvent and extracted for 1 to 12 hours with an ultrasonic extractor at room temperature. The time of the ultrasonic extraction may vary depending on the amount of the sample to be extracted. Preferably, 10 g to 1000 ml of extraction solvent is used for 1 g of red ginseng.

In particular, the extraction process according to the present invention, as the extraction process, is performed at a lower temperature than the hot water extraction, which was conventionally applied heat around 100 ℃ to maximize the extraction of the active ingredient in red ginseng.

Preferably, the reaction is carried out at 50 to 90 ° C. for 1 to 12 hours, more preferably at 70 to 90 ° C. for 3 to 10 hours.

In the present extraction process, if the extraction temperature is less than the above temperature / hour, the active ingredient in red ginseng may not be sufficiently extracted. On the contrary, if the extraction temperature exceeds the above temperature / time, the active ingredient in red ginseng may be destroyed, so it is suitably used within the above range.

(S2) low temperature filtration process

Next, a process of cooling and filtering the extract obtained through the extraction process is performed.

Specifically, the extract is cooled to a temperature of 1 ~ 30 ℃, and then subjected to a low temperature filtration process divided into four or more times for each temperature.

Specifically, the process may be performed by cooling to 25 to 30 ° C., performing a filtration process once at this temperature, and then performing a low temperature filtration process whenever cooling is performed at intervals of 5 to 10 ° C. In one example, the filtration process is performed at 30 ° C, 20 ° C, 10 ° C, and 1 ° C.

In the case of conventional filtration, many precipitates are produced at the same time, and the extraction yield is extremely reduced. In the present invention, this problem can be solved through a multi-stage filtration process, and at the same time, the extraction rate of the active ingredient in red ginseng can be improved.

Filtration is carried out using a centrifuge. Filtration using a centrifugal separator can effectively separate particles, ie precipitates and extracts, by centrifugal force. The centrifuge is not particularly limited in the present invention, a well-known tubular (disc or bowlcentrifuge) centrifugal separator can be used, the rotation speed is 1000 to 5000rpm, preferably 1500 to 1500 Can be used in the range of 3000rpm, it is carried out for 30 minutes to 1 hour.

(S3) low temperature concentration process

Next, the filtrate obtained in the above step is concentrated at low temperature.

Concentration is carried out under reduced pressure using a vacuum evaporator, for example, a rotary evaporator to remove the extraction solvent to obtain a high concentration red ginseng concentrate containing the active ingredient.

In particular, the concentration in the present invention is carried out at low temperature for 1 to 24 hours at 50 to 70 ℃, preferably 3 to 10 hours at 55 to 65 ℃. When the temperature is lower than the above range during the low temperature concentration process, the extraction solvent may not be effectively removed or the concentration time may be longer than necessary for the removal of the extraction solvent, resulting in a long process time. On the contrary, if the temperature exceeds the above range, the active ingredient having a low volatilization temperature in the red ginseng concentrate is removed as well, and thus the extraction efficiency is greatly reduced.

(S4) Low Temperature Aging Process

Next, the concentrate obtained in the above step is aged at low temperature to obtain a red ginseng concentrate.

Aging is carried out at a low temperature warehouse for 10-20 days at 10-40 ° C. to obtain a red ginseng concentrate with the natural aroma and soft taste of red ginseng.

The red ginseng concentrate prepared as described above contained 25.38 mg / g of eight ginsenosides Ra1, Rb1, Rb2, Rc, Rd, Re, Rf, and Rg1 through Experimental Example 1, and the amino acid asparagine (asparagine) , Serine, glutamine, glycine, histidine, arginine, arginine, threonine, alanine, proline, cystine, tyrosine , Valine, methionine, lysine, isoleucine, leucine, and phenylalanine contain 21.49 mg / g, AFG (arginyl-fructosyl-glucose) Arginine-Frictose-Glucose) was included in an amount of 69.01 mg / g, and it was confirmed that the active ingredient contained in a high content compared to any conventionally known red ginseng concentrate. In addition, other components, acidic polysaccharides (RGAP) and minerals, are present in a good balance.

In addition, the red ginseng concentrate according to the present invention through Experimental Example 2 significantly improved the immune enhancing effect, and confirmed through the Experimental Example 3 also has the effect of improving memory.

This red ginseng concentrate can be used for various purposes.

For example, the red ginseng concentrate may be used as a health functional food, wherein the health functional food may be used as the red ginseng concentrate itself or may include a food supplement acceptable food additive.

In addition, the red ginseng concentrate of the present invention can be used in addition to food or beverage, wherein the amount of the red ginseng concentrate in the food or beverage can be added to 0.01 to 15% by weight of the total food weight, the health beverage composition based on 100 ml In the range of 0.02 to 5 g, preferably 0.3 to 1 g.

[Example]

Hereinafter, preferred embodiments and experimental examples are provided to facilitate understanding of the present invention. However, the following examples are provided only for the purpose of easier understanding of the present invention, and the present invention is not limited by the examples.

Example 1 Preparation of Red Ginseng Concentrate

Purified water (500ml compared to 1g of red ginseng) was added so that red ginseng (Korea Ginseng Corp., 6 years old) was sufficiently immersed, and then it was put in a constant temperature water bath (Multi water bath, Visiontec) and performed extraction process at 50 ° C for 12 hours.

Subsequently, the extract was cooled to 30 ° C., followed by primary centrifugation at 1500 to 1800 rpm for 30 minutes, again to 20 ° C., followed by secondary centrifugation under the same conditions, followed by cooling to 20 ° C., followed by 3 After centrifugation, the mixture was cooled to 1 ° C, and then subjected to filtration by secondary centrifugation under the same conditions.

Subsequently, the obtained filtrate was placed in a vacuum concentrator, concentrated at 55 ° C. for 10 hours, and then aged at 20 ° C. for at least 20 days at low temperature to obtain a red ginseng concentrate.

Comparative Example 1: Preparation of Red Ginseng Concentrate

The red ginseng concentrate was obtained by the conventional method.

Purified water (900ml compared to 1g of red ginseng) is added to immerse red ginseng (Korea Ginseng Corp., 6 years old) sufficiently and put it in a constant temperature water bath (Multi water bath, Visiontec) and perform hot water extraction process at 95 ℃ for 12 hours. It was.

The extract was then cooled to 50 ° C. and then filtered.

Subsequently, the obtained filtrate was placed in a vacuum concentrator, and then concentrated at 80 ° C. for 10 hours to obtain a red ginseng concentrate.

Experimental Example 1: Component Analysis of Red Ginseng Concentrate

The content of the active ingredient in the red ginseng concentrate prepared in Examples and Comparative Examples was analyzed, and the results are shown in Tables 1 to 3 below.

(1) Ginsenoside content: was analyzed at 203nm using BEH C18 (2.1 × 500 mm, 1.7μm, Waters), using UPLC (Waters), the results are shown in Table 1, Figures 1 and 2 Shown in 1 is Example 1 of the present invention, Figure 2 is a ginsenoside-related chromatogram of the red ginseng concentrate obtained in Comparative Example 1.

mg / g Example 1 Comparative Example 1 Ra1 1.69 1.33 Rb1 8.99 6.59 Rb2 3.36 2.61 Rc 3.84 2.87 Rd 1.13 1.07 Re 2.75 1.52 Rf 1.58 1.59 Rg1 2.04 1.07 total 25.38 18.67

Referring to Table 1, Figures 1 and 2, the red ginseng concentrate of Example 1 and Comparative Example 1 showed a result satisfying the 0.8 ~ 34 mg / g of ginseng or red ginseng concentrate standard of the current health functional foods.

However, the red ginseng concentrate of Example 1 showed a result of increasing the content of ginsenoside by about 36% from 18.67 mg / g to 25.38 mg / g compared to Comparative Example 1, the red ginseng concentrate through the extraction process according to the present invention It can be seen that the content of ginsenosides can be increased.

(2) Amino acid content: measured using HPLC (Waters), where Discovery C18 (4.6 × 250 mm, 5 μm), Supelco as a column, Fluorescence Detector (Excitation wavelenght 250 nm, Emission wavelenght 395 nm) as a detector Was used, and the results are shown in Table 2, and FIGS. 3 and 4. Figure 3 is a chromatogram of amino acids of the red ginseng concentrate obtained in Example 1, Figure 4 of the present invention.

mg / g Example 1 Comparative Example 1 Asparagine 1.30 0.77 Serine 0.21 0.12 Glutamine 0.47 0.29 Glycine 0.18 0.15 Histidine 0.50 0.46 Arginine) 16.44 9.03 Threonine 0.35 0.26 Alanine 0.78 0.69 Proline Not detected Not detected Cystine Not detected Not detected Tyrosine 0.34 0.27 Valine 0.22 0.19 Methionine Not detected Not detected Lysine 0.16 0.04 Isoleucine 0.13 0.09 Leucine 0.23 0.16 Phenylalanine 0.19 0.11 total 21.49 12.61

Referring to Table 2, FIG. 3 and FIG. 4, the content of amino acids also increased by about 70% from 12.61mg / g to 21.49mg / g. In particular, the contents of the essential amino acids threonine, valine, methionine, lysine, isoleucine, leucine, and phenylalanine also increased significantly.

(3) AFG (arginine-fructose-glucose), acidic polysaccharide content analysis: CarboPac PA-1 Column (4 × 250 mm), Doinex column using Bio-LC (Dionex), PAD (Pulse) as detector It was measured using an amperometric detector, and the results are shown in Table 3, FIGS. 5 and 6. FIG. 5 is a diagram illustrating AFG-related chromatogram of red ginseng concentrate obtained in Example 1 and FIG. 6 of Comparative Example 1. FIG.

mg / g Example 1 Comparative Example 1 AFG (arginine-fructose-glucose) 69.01 14.75 Acidic polysaccharides 57.66 59.89

Referring to Table 3, Figure 5 and Figure 6, the AFG content also showed an increase of about 368% or more from 14.75mg / g to 69.01mg / g. Acidic polysaccharides were also measured at appropriate levels.

Experimental Example 2: Immune Enhancement Effect of Red Ginseng Extract

In order to examine the immune enhancing effect of the red ginseng concentrate according to the present invention, the antibody producing ability, the immune cell number responsible for the immune action, and IL-4 secretion ability were measured.

(1) antibody production ability:

Normal group, cyclophosphamide (CY) Control, red ginseng concentrate 250 of Comparative Example 1, 500 mg / kg administration group, red ginseng concentrate 250 of Example 1, 500 mg / kg administration group was administered orally daily for 10 days. Five days before necropsy, 50 mg / kg of CY was intraperitoneally administered, and the normal group was intraperitoneally administered with saline. Four days before necropsy, all groups were intraperitoneally administered 5 ml of sheep red blood cells (SRBC) to induce an immune response. In order to measure the IgM hemolytic plague forming cell number (AFC) of splenocytes, cotton sheep blood purchased from South Pacific Sera Ltd. (Timaru, New Zealand) was refrigerated and used within 1 week. 50 mg / kg of CY was intraperitoneally administered 5 days before the necropsy (day 5), and the normal group was intraperitoneally administered with saline. After 4 days of autopsy (day 6), SRBC was centrifuged three times (1200 rpm, 10 min, 4 ° C) with EBSS solution, and then the sheep red blood cell (SRBC) concentration was 5 × 10 ⁸ cells / ml ( 0.5 ml of this suspension was injected intraperitoneally in all experimental animals to induce an immune response. On the day of necropsy (day 10), the spleens of all experimental animals were removed and placed in an ice-cold EBSS solution to make the spleens into a single cell suspension using a mesh. Remove the supernatant by centrifugation (1200 rpm, 10 min, 4 ° C), add 5 ml EBSS solution, remove the supernatant by centrifugation (1200 rpm, 10 min, 4 ° C), and add 3 ml EBSS solution. Made. 100 ul of this was added to a 2.9 ml EBSS solution to make a cell suspension diluted 30-fold. SRBC suspension was used after centrifuged washing (1200 rpm, 10 min, 4 ° C.) three times with EBSS solution immediately before using the stored sheep blood. 350 ul of Agar, 25 ul of SRBC suspension, 25 ul of guinea pig complement, and 100 ul of cell suspension were mixed and dropped to 200 ul on a plate and covered with a cover glass. After 4 hours of incubation at 37 ° C. in a 5% CO ₂ incubator, the number of hemolytic plague-producing cells formed was measured under a microscope.

(2) Spleen lymphocyte distribution map:

Normal group, cyclophosphamide (CY) Control, red ginseng concentrate 250 of Comparative Example 1, 500 mg / kg administration group, red ginseng concentrate 250 of Example 1, 500 mg / kg administration group was administered orally daily for 10 days. Immunization was induced by intraperitoneal administration of 50 mg / kg of CY 3 days before necropsy. On the day of necropsy, the spleens of the test animals were extracted and made into a single cell suspension using mesh. The supernatant was removed by centrifugation (1200 rpm, 10 min, 4 ° C.), 2 ml ACK lysis buffer was added, and then shaken for 1 minute to hemolyze. 5 ml of RPMI 1640 complete (10% FBS, 2 mM L-glutamine, 100 unit penicillin, 100 ug streptomycin, and 5 × 10 ⁻⁵ 2-Mercaptoethanol) were added and allowed to stand at room temperature for 5 minutes. The supernatant was removed by centrifugation (1200 rpm, 5 min, 4 ° C.) and washed twice with RPMI 1640 culture to make single cell suspension. Composition ratio of T cells to spleen single cell suspension was measured using a flow cytometry (Beckman coulter, FC500-2 laser 5 color) equipment. Total cell number was measured by coulter counter (Beckman coulter, Vi-cell- Tryphan Blue method full automation). Purified anti-mouse CD16 / CD32 Fc receptor was used for Fc blocking, and total T cells were stained with peridinin chlorophyll-a protein (PerCP) -conjugated anti-mouse CD3e (clone: 145-2C11). FITC-conjugated anti-mouse CD4 (clone: GK1.5) and R-PE-conjugated anti-mouse to determine the ratio of CD4 T cells (helper T cells) and CD8 T cells (suppressor / cytotoxic T cells) in the spleen Staining was performed using CD8a (clone: 53-6.7). Briefly, the method was first blocked with anti-mouse CD16 / CD32 Fc receptor (1 ug / tube) at 4 ° C. for 15 minutes. Two anti-mouse monoclonal antibodies (1 ug / tube) were combined and stained on ice for 30 minutes and washed three times with PBS. Then, 0.3 ml of PBS was added to each tube, mixed well, and analyzed by flow cytometry.

(3) IL -4 Secretory function :

Normal group, cyclophosphamide (CY) Control, Red ginseng concentrate 250 of Comparative Example 1, 500 mg / kg administration group, Red ginseng concentrate 250 of Example 1, 500 mg / kg daily forced oral administration for 10 days, 50 days before the autopsy Spleens were isolated from mice intraperitoneally administered mg / kg of CY to obtain single cell suspensions. Adjust the concentration of single cell solution to 1 × 10 ⁶ cells / ml, dispense 180 ul of cell solution into a 96-well culture dish, add 20 ul of Con A (5 ug / ml) to a total volume of 200 ul. Unified. After 72 hours of incubation at 37 ° C. in a 5% CO ² incubator, at least 50 ul of each medium was collected and stored at −70 ° C., and each of the cytokines released in this medium was treated for mouse IL-4 (invitrogen). Measurement was performed using an ELISA kit.

(1) to (3) was performed by dividing into a normal group, immune lowering group, immune lowering group (administration of red ginseng concentrate of Comparative Example 1), immune lowering group (administration of red ginseng concentrate of Example 1), Table 4 shows.

Antibody Production Performance Immune cell count
IL-4 secretion capacity (pg / ml)
AFC / 10 ⁶ spleen cell T-cell count
(× 10 ⁷ ) CD4-T-cell count
(× 10 ⁷ ) CD8-T-cell count
(× 10 ⁷ ) Normal group 2400 0.82 0.58 0.14 340 Immune suppression group 847 ** 0.31 ** 0.23 ** 0.05 ** 205 ** Red ginseng concentrate 250 mg / kg of immune lowering group + Comparative Example 1 1308 ** ## 0.50 ** ## 0.38 ** # 0.09 ** # 224 ** Red ginseng concentrate 500 mg / kg of immune lowering group + Comparative Example 1 1722 * ## 0.51 ** ## 0.39 ** ## 0.08 ** # 330 ## Red ginseng concentrate 250 mg / kg of the immune-lowering group + Example 1 1503 ** ## 0.46 ** ## 0.34 ** # 0.08 ** # 272 ** ## Red ginseng concentrate 500 mg / kg of immune-lowering group + Example 1 1826 * ## 0.58 ** ## 0.45 ** ## 0.09 ** ## 339 ##

** P <0.01: normal vs immunocompromised,

#P <0.05, ## P <0.01: immunocompromised vs red ginseng concentrate group

Referring to Table 4, in the case of the red ginseng concentrate according to the present invention it can be seen that the immune enhancing effect is excellent.

Experimental Example  3: memory improvement effect of red ginseng concentrate

In order to determine the memory improvement effect of the red ginseng concentrate according to the present invention, the memory improvement effect and the spatial cognition improvement effect were measured.

(1) memory improvement effect

The passive avoidance reaction was carried out using a step-down type test apparatus (Muromachi Co., Ltd.). The three sides were made of black, one side made of transparent acrylic, and the bottom was made of an electrically conductive grid of 0.2 cm thick. It became. When the mouse was placed on the platform located at one corner of the floor and descended to the floor, a 0.4 mA electric shock was applied for 2 seconds. Mice with a latency of 3 to 15 seconds (no electrical shock to the grid at this time) were selected to primarily select each group. To reduce memory, scopolamine 2mg / kg was injected subcutaneously, and was divided into normal group, control group (scopolamine group), red ginseng concentrate (100mg / kg) + scopolamine, red ginseng concentrate (250mg / kg) + scopolamine group.

The red ginseng concentrate was administered orally for 7 days, and the first study was conducted 30 minutes after the administration of the red ginseng concentrate on the last 7 days. Memory regeneration was performed 24 hours after the second study. Amnesia was injected subcutaneously with 2 mg / kg of scopolamine 15 minutes after the second study. A latency limit of 120 seconds was applied.

(2) spatial cognition Morris water maze test

Indicate the orientation of the north, south, east, and west of a 90 cm diameter tank and fill it with water (water temperature 26 ± 1 ° C) so that the depth is 30 cm. Install in cm. In order to make the animal identify the platform, 1000 ml of milk was added to the water and mixed evenly. The animals were placed in a water tank in four directions (east, west, south, and north), and the time (elapsed time) to reach and swim by the platform in the southwest direction was measured. To reduce memory, scopolamine 2 mg / kg was injected subcutaneously 30 minutes after 250 mg / kg oral administration of red ginseng concentrate. After 30 minutes of subcutaneous injection, the time of arrival at the platform fixed at the center in the southwest direction from the northwest, northwest direction was measured for 3 days. The experimental animals exceeding the time limit of 2 minutes were artificially placed on the platform for 5 seconds to recognize the platform. During the experiment, animal experiments from the labyrinth were drained and transferred to breeding cages. All experimental groups used 5 experimental animals per group. The values obtained in four directions per day were added and used as the daily average. At this time, the spatial cognition was measured for 3 days in the normal group, memory lowering group, red ginseng concentrate + memory lowering group.

Memory Improvement Effect of Red Ginseng Extract by Passive Avoidance Test group Latency time (seconds) Normal group 81.37 Memory loss 35.28 ** Memory lowering group + Comparative Example 1 Red ginseng concentrate (100mg / kg) 91.61 ## Memory lowering group + Comparative Example 1 Red ginseng concentrate (250mg / kg) 89.43 ## Memory lowering group + Example 1 Red ginseng concentrate (100 mg / kg) 97.28 ## Memory lowering group + Example 1 Red ginseng concentrate (250 mg / kg) 107.97 ##

** P <0.01: normal group vs. memory loss group, ## P <0.01: memory group vs red ginseng concentrate group

Spatial Cognitive Effect of Red Ginseng Extract Test group Escape Time (sec) 0 days 1 day 2 days 3 days Normal group 52.1 39.6 24.1 15.3 Memory loss 80.6 99.9 ** 75.3 ** 65.2 ** Red ginseng concentrate (250mg / kg) of memory lowering group + Comparative Example 1 79.6 71.5 # 60.7 48.9 Red Ginseng Extract (250mg / kg) in Memory Lowering Group + Example 1 77.3 68.6 # 39.4 # 31.3 #

** P <0.01: normal group vs. memory loss group, # P <0.05: memory group vs. red ginseng concentrate group

Referring to Tables 5 and 6, it can be seen that the red ginseng concentrate according to the present invention has an excellent memory improvement effect.

As described above, in the present invention, the low-temperature method was performed to increase the content of ginsenosides, amino acid derivatives, and AFG components in red ginseng, thereby increasing immunity and improving memory.

Red ginseng concentrate according to the present invention has a high content of the active ingredient is excellent immunity enhancement effect and memory improvement effect can be usefully used as a functional health food to prevent various diseases and diseases and maintain health.

Claims (11)

Extracting red ginseng by adding to the extraction solvent;
Cooling the obtained extract and performing low temperature filtration at multiple stages at 1 to 30 ° C;
Low temperature concentration of the obtained filtrate at 50 ~ 70 ℃; And
A method for producing a red ginseng concentrate comprising the step of aging the obtained concentrate at 10 to 40 ℃ low temperature. The method according to claim 1, wherein the red ginseng is red ginseng powder or dried red ginseng. The preparation of red ginseng concentrate according to claim 1, wherein the extraction solvent comprises one selected from the group consisting of water, a lower glycol having 1 to 6 carbon atoms, a lower alcohol having 1 to 4 carbon atoms, and a mixed solvent thereof. Way. The method for preparing a red ginseng concentrate according to claim 1, wherein the extraction is carried out using 10 to 1000 ml of extraction solvent for 1 g of red ginseng. The method of claim 1, wherein the extraction is performed for 1 to 12 hours at 50 to 90 ℃ red ginseng concentrate production method. delete The red ginseng according to claim 1, wherein the low temperature filtration is performed by dividing at least four times for each temperature, and cooling once at 25 to 30 ° C., and filtering at each time of cooling at intervals of 5 to 10 ° C. Method for preparing the concentrate. The method of claim 1, wherein the low temperature filtration is performed using a centrifuge. The method of claim 1, wherein the low temperature concentration is carried out for 1 to 24 hours. The method of claim 1, wherein the low-temperature aging is carried out for 10 to 20 days. The method of claim 1, wherein the cold filtration is a method for producing a red ginseng concentrate, characterized in that the filtration at 30 ℃, 20 ℃, 10 ℃, and 1 ℃.

Images