KR101298119B1 - Composition comprising extract of mycelium or fruit body of Pleurotus cystidiosus having anti-aging and anti-wrinkle activity - Google Patents

Abstract

The present invention comprises an anti-aging and skin wrinkle improvement cosmetic composition containing an extract of Pleurotus cystidiosus mycelium or fruiting body as an active ingredient; Provided are pharmaceutical compositions and dietary supplements. The extract of the abalone elder mycelium or fruiting body of the present invention is excellent in anti-aging and skin wrinkle improvement effect due to inhibition of elastase activity and is safe as a natural material, and can be used in various cosmetic compositions, pharmaceutical compositions and health functional foods.

Description

Composition comprising extract of mycelium or fruit body of Pleurotus cystidiosus having anti-aging and anti-wrinkle activity}

The present invention relates to a cosmetic composition, a pharmaceutical composition, and a health functional food for anti-aging and skin wrinkle improvement, containing an extract of Pleurotus cystidiosus mycelium or fruiting body, which inhibits elastase activity.

Skin is an important organ that protects the human body from the external environment and is in charge of various physiological functions such as barrier function, temperature control, and excretion that prevent the internal moisture and useful ingredients from leaking out. However, as you age, these functions gradually weaken and your skin ages. In particular, when the lipid composition and content of the lipid layer, which functions as a skin barrier, changes rapidly, the function decreases rapidly and the skin moisture content decreases, resulting in dry skin, blemishes, freckles, pigmentation and various skin lesions. The connective tissue involved in skin wrinkle formation is mainly composed of fiber components such as collagen fiber, reticulated fiber, elastin fiber and the like. Collagen and elastin in the dermal tissue are important fibrous tissues that work closely together to maintain skin flexibility and elasticity.

Elastin is a major fibrous tissue that affects the elasticity of the skin. The amount of elastin is about 2%, which is a small amount compared to collagen, but it plays an important role in skin elasticity because it forms a network tissue supporting the epidermis. Elastase is known to be the only degrading enzyme that degrades elastin, an insoluble elastic fibrous protein in animal connective tissue that maintains skin elasticity (Wiedow, O., Schroder, JM, EJ Biol. Chem., 265 (5), p14791, 1990). Elastase is overproduced by increasing age or by external skin irritation such as ultraviolet rays. Over-produced elastase excessively breaks down elastin, weakening collagen-to-collage bonds and creating wrinkles on the skin. In particular, after the age of 40, the elasticity of the skin is drastically reduced due to the action of elastase, as the action of the elastase increases with age, the elastin fibers are partially lost or aggregated, and the collagen fibers are reduced. to be. By inhibiting the activity of elastase, skin aging and skin wrinkles can be suppressed (Imokawa, G. (2001) The role of elastase secreted by fibroblasts in wrinkle formation; implication through selective inhibition of elastase activity.Photochem. Acd Phptobiol. 74: 283-287; Bissett, DL, Photochem.Photobiol., 46, pp 367-378, 1987; Bizot-Foulon V., Godeat G., W. Int. J. Cos. Sci., 17, pp 255 -264, 1995).

At present, extracts used in cosmetics for skin aging and wrinkle improvement due to elastase inhibitory activity include cedar extract (patent application 10-1998-0047619), yew extract (patent application 10-2001-0041646), Chomi extract (patent application 10-2008-0048124), Ecklonia cava extract (patent application 10-2002-0050331), pueraria myripica extract (patent application 10-2000-0012539), betel nut extract (patent application 10-2001-0029429) , Red bean pear leaf extract (patent application 10-2007-0131283), sun rock fern extract (patent application 10-2008-0030150), bear horsetail extract (patent application 10-2008-0018217), green leaf extract (patent application 10- 2007-0091925), haveby fern extract (patent application 10-2008-0030151), wildberry extract (patent application 10-2007-0042739), extract of vine plants (patent application 10-2003-0019426) and the like are disclosed have.

Meanwhile, vascular protection and blood circulation disorder disease prevention / treatment use due to inhibition of elastase activity is disclosed in Korea Patent No. 10-0717996, and Korean Patent Application for Prevention / Treatment of respiratory disease caused by inhibition of elastase activity. 10-2003-0016214, which is disclosed in Korean Patent Application Publication No. 10-2005-0058283 for the prophylaxis / treatment of diseases related to cell necrosis due to inhibition of elastase activity.

Pleurotus cystidiosus belongs to the Plerotaceae family, and is named for its flesh similar to abalone, also called miller beetle (named Pleurotus cystidiosus by OK Miller), maple beetle, and Taiwan beetle. . Common echidna are mid- and low-temperature plants with a cultivation temperature of less than 25 ℃, while abalone shells also occur in high temperatures of 25-30 ℃. The optimal cultivation time in Korea is when the average temperature is 20 ~ 28 ℃, that is, from mid June to early September. The morphological features of abalone elk are flat in shape, 6.6 to 9.8 cm in size, and 1.6 to 3.8 cm in length. . It is not formed in the form of a bundle like a circular blade, but is generated individually, and the average is about 12.8g. The lampshade is dark brown in color and is off-white, but the color is dark and light due to temperature and light.

Accordingly, the present inventors have conducted research to search for substances that help to improve skin aging and wrinkles. As a result, the present inventors confirmed the effects of skin aging and wrinkle improvement due to elastase inhibitory activity from extracts of abalone elder mycelium or fruiting body, and completed the present invention. It was.

An object of the present invention is to provide an anti-aging and skin wrinkle improvement cosmetic composition containing an extract of abalone elder mycelium or fruiting body as an active ingredient.

Another object of the present invention is to provide an anti-aging and anti-wrinkle pharmaceutical composition containing an extract of abalone mycelium or fruiting body as an active ingredient.

It is another object of the present invention to provide an anti-aging and skin wrinkle improvement health functional food containing an extract of abalone elder mycelium or fruiting body as an active ingredient.

As one aspect of the present invention, the present invention provides a cosmetic composition for anti-aging and skin wrinkle improvement containing an extract of Pleurotus cystidiosus mycelium or fruiting body as an active ingredient. The extract of the abalone mycelium or fruiting body inhibits elastase activity, thereby acting as anti-aging and improving skin wrinkles.

The extract of abalone mycelium or fruiting body may be extracted with water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.

In the cosmetic composition of the present invention, the abalone mycelium or fruiting body extract may be contained in 0.001 to 10% by weight based on the total weight of the composition, preferably 0.01 to 1% by weight.

The cosmetic composition includes skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, pack, soap, cleansing It may be formulated into a foam, cleansing lotion, cleansing cream, body lotion or body cleanser and the like.

As another aspect of the present invention, the present invention provides a pharmaceutical composition for anti-aging and skin wrinkle improvement containing an extract of abalone elder mycelium or fruiting body as an active ingredient.

The pharmaceutical composition may be used in any external skin preparation that can be used in the art, and specifically, as a cream, gel, patch, spray, ointment, warning agent, lotion, linen agent, pasta agent or cataplasma agent, etc. Can be formulated.

As another aspect of the present invention, the present invention provides an anti-aging and anti-wrinkle health functional food containing an extract of abalone elder mycelium or fruiting body as an active ingredient.

The dietary supplement may be used in any formulation usable in the art, and may be formulated into pills, powders, granules, acupuncture, tablets, capsules or beverages.

As another aspect of the present invention, the present invention provides a pharmaceutical composition for preventing or treating a blood circulation disorder disease containing an extract of abalone elder mycelium or fruiting body as an active ingredient.

The blood circulation disorder includes, but is not limited to, arteriosclerosis, hypertension, heart disease, peripheral vascular disease, hyperlipidemia, myocardial infarction, thrombosis, ischemia, venous insufficiency, and angina pectoris.

As another aspect of the present invention, the present invention provides a pharmaceutical composition for preventing or treating respiratory diseases comprising an extract of abalone mycelium or fruiting body as an active ingredient.

The respiratory diseases include, but are not limited to, systemic inflammatory response syndrome, asthma, emphysema, bronchitis, acute lung injury, acute respiratory distress syndrome, idiopathic pulmonary fibrosis, pneumonia, pulmonary edema, lung cancer.

As another aspect of the present invention, the present invention provides a pharmaceutical composition for preventing or treating a cell necrosis-related disease containing an extract of abalone mycelium or fruiting body as an active ingredient.

The cell necrosis-related diseases include, but are not limited to, neurodegenerative diseases, leukemia, lymphoma, neonatal respiratory distress syndrome, diabetes mellitus, tuberculosis, endometriosis, psoriasis, hepatitis, ischemic heart disease, ischemic stroke, sepsis, pancreatitis, and hyperthermia Includes Crohn's disease, multiple sclerosis, frostbite, burns.

As another aspect of the present invention, the present invention provides a food additive for anti-aging and skin wrinkle improvement containing an extract of abalone elder mycelium or fruiting body as an active ingredient.

The extract of the abalone elder mycelium or fruiting body of the present invention is excellent in anti-aging and skin wrinkle improvement effect due to inhibition of elastase activity and is safe as a natural material, and can be used in various cosmetic compositions, pharmaceutical compositions and health functional foods.

Hereinafter, the components and technical features of the present invention will be described in more detail with reference to the following examples. However, the following examples are intended to illustrate the contents of the present invention and are not intended to limit the scope of the invention. The documents cited in the present invention are incorporated herein by reference.

Example

Example  One: Abalone  Preparation of extracts of mycelium or fruiting body

Sterilized distilled water was put in a petri dish containing cellophane, soaked cellophane, and then tucked up one by one on a ME agar plate using tweezers. Abalone mycelia (deposited to the culture collection and DNA Bank of Mushroom (CCDBM) using sterile scarpel (accession number: IUM01232, date of deposit: February 04, 2009) The grown agar block was cut to a size of 0.5 x 0.5 mm and placed in the center of the ME agar plate and the ME agar plate loaded with the cellophane.The strains of abalone lodge were found on the website of the Mushroom Strain and DNA Bank (CCDBM). Application can be made at http://www.wildmush.or.kr/sub/catalog.php?CatNo=23&Sort =) Depending on the strain, it was incubated at 20-30 ℃ (standard condition 22 ℃) for 1-2 weeks. Using a spatula wiped with alcohol, the hyphae grown on the cellophane was scraped and placed in a translucent brown tube.The fruiting body was chopped into small pieces, and 1 ml of methanol was added to each tube. up-down After one week or more, the mycelium was allowed to settle and the supernatant was transferred to a 2 ml centrifuge (labeled before weighing the solvent and weighed and recorded with an empty tube). In this case, 1 ml of methanol was added and the supernatant after up-down and centrifugation was collected in the previous tube, methanol was evaporated at speed vac, and the total weight of the tube and the extract was measured and recorded. Extracts of the fruiting bodies were stored dry at -20 ° C until needed.

Other extract samples for the test of Example 2 were also prepared in the same manner as above.

Example  2: Elastase  Activity inhibition effect measurement

The effect of inhibiting the activity of elastase, which inhibits skin elasticity, was tested for certain substances. This test method is an in vitro measure of skin anti-aging and is primarily intended for the screening of inhibitors that inhibit the enzyme enzyme elastase that degrades elastin, which plays an important role in maintaining skin elasticity. The principle of measurement is that when PPE (Porcine pancreatic elastase) (Sigma, USA) decomposes the synthetic substrate N-Succinyl Trialanine P-Nitroanilide (Sigma, USA), it produces p-nitroaniline, which develops yellow color. The extent is measured with an absorbance photometer to calculate PPE inhibition.

First, the extract was dissolved in DMSO and diluted to meet the required concentration. The buffer solution was prepared by adjusting 0.26M Tris solution to pH8.0 with 0.267M hydrochloric acid solution. 4 ml of elastase substrate N-Succinyl Trialanine P-Nitroanilide was added to 1 ml of the buffer solution to make 8.8 mM (substrate). Porcine pancreatic elastase (Porcine Pancreatic Elastase) was made at a concentration of 10 ug / ml in buffer (enzyme solution). 60ul of buffer, 20ul of substrate, 1ul of sample, and 20ul of enzyme were mixed and reacted at 25 ° C for 15 minutes. The control group used DMSO instead of the sample. Then, absorbance was measured at 410 nm.

The inhibition rate of elastase activity was calculated by the following equation.

[Equation 1]

Elastase activity inhibition rate (%)

= (Absorbance of control group-absorbance of experimental group) / absorbance of control group X 100

Elastase activity inhibition rate is shown in Table 1 below.

density
(W / V) ICNP05 ICNP02 ICNP01 G2 D6 D9 E11 D5 0.04% 0.7 -12.2 -46.3 -39.8 -44.2 0.1% 42.1 -1.9 -36.8 -28.4 -28.4 0.3% 80.9 24.7 -32.1 -17.6 -31.8 0.6% 78.0 2.1 0.7 10.0 6.3

As shown in Table 1, it can be seen that the elastase activity inhibition rate of the extract of the abalone elder mycelium or fruiting body of the present invention (ICNP05-G2) is significantly superior to other samples.

Hereinafter, the formulation example of the cosmetic composition containing the extract of abalone elder mycelium or fruiting body of the present invention will be described.

Formulation Example  1. lotion ( skin )

Raw material Example 1 Comparative Formulation Example 1 One Abaloneweed Extract (Example 1) 0.1 - 2 1,3-butylene glycol 2.00 2.00 3 glycerin 3.00 3.00 4 Oleyl alcohol 2.00 2.00 5 Polysorbate 20 1.00 1.00 6 ethanol 6.00 6.00 7 antiseptic a very small amount a very small amount 8 Combination fragrance a very small amount a very small amount 9 Purified water Balance Balance system 100.00 100.00

Formulation Example  2. Nourishing Cosmetic (Lotion)

Raw material Execution Formulation Example 2 Comparative Formulation Example 2 One Abaloneweed Extract (Example 1)  0.1 - 2 1,3-butylene glycol 4.00 4.00 3 glycerin 3.00 3.00 4 Oleyl alcohol 0.05 0.05 5 Polysorbate 20 1.00 1.00 6 antiseptic a very small amount a very small amount 7 Combination fragrance a very small amount a very small amount 8 Purified water Balance Balance system 100.00 100.00

Formulation Example  3. Nourishing Cream

Raw material Example 3 Comparative Formulation Example 3 One Abaloneweed Extract (Example 1)  0.1 - 2 glycerin 5.00 5.00 3 Triethanolamine 0.50 0.50 4 Tocopheryl acetate 1.00 1.00 5 Liquid paraffin 5.00 5.00 6 Squalane 5.00 5.00 7 Macadamia Nut Oil 15.00 15.00 8 Polysorbate 60 1.00 1.00 9 Sorbitan sesquioleate 1.00 1.00 10 Carboxyl vinyl polymer 0.20 0.20 11 antiseptic a very small amount a very small amount 12 Combination fragrance a very small amount a very small amount 13 Purified water Balance Balance system 100.00 100.00

Hereinafter, the formulation example of the pharmaceutical composition containing the extract of abalone elder mycelium or fruiting body of the present invention will be described.

Formulation Example  4. Powder

Raw material Example 4 One Abaloneweed Extract (Example 1) 300 mg 2 Lactose 100 mg 3 Talc 10 mg

Formulation Example  5. Tablet

Raw material Example 5 One Abaloneweed Extract (Example 1) 300 mg 2 Corn starch 100 mg 3 Lactose 100 mg 4 Magnesium stearate 2 mg

Formulation Example  6. Capsule

Raw material Example 6 One Abaloneweed Extract (Example 1) 300 mg 2 Corn starch 100 mg 3 Lactose 100 mg 4 Magnesium stearate 2 mg

Formulation Example  7. Injection

Raw material Example 7 One Abaloneweed Extract (Example 1) 300 mg 2 Sterile sterilized water for injection Suitable amount 3 pH adjusting agent Suitable amount

Hereinafter, an example of the formulation of a health functional food containing an extract of abalone elder mycelium or fruiting body of the present invention will be described.

Formulation Example  8. Healthy food

Raw material Example 8 One Abaloneweed Extract (Example 1) 1000 mg 2 Vitamin mixture Suitable amount 3 Vitamin A acetate 1.0 mg 4 Vitamin E 0.13 mg 5 Vitamin B1 0.15 mg 6 Vitamin B2 0.5 mg 7 Vitamin B6 0.2 ug 8 Vitamin B12 10 mg 9 Vitamin C 10 ug 10 Biotin 1.7 mg 11 Nicotinic acid amide 50 ug 12 Folic acid 0.5 mg 13 Calcium pantothenate Suitable amount 14 Mineral mixture Suitable amount 15 Ferrous sulfate 1.75 mg 16 Zinc oxide 0.82 mg 17 Magnesium carbonate 25.3 mg 18 Potassium monophosphate 15 mg 19 Dibasic potassium phosphate 55 mg 20 Potassium citrate 90 mg 21 Calcium carbonate 100 mg 22 Magnesium chloride 24.8 mg

Formulation Example  9. Healthy Drink

Raw material Example 9 One Abaloneweed Extract (Example 1) 1000 mg 2 Citric acid 1000 mg 3 oligosaccharide 100 g 4 Plum concentrate 2 g 5 Taurine 1 g 6 Purified water Suitable amount

While the present invention has been described with reference to exemplary embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof without departing from the scope of the invention. You will know. In addition, many modifications may be made to adapt a particular situation and material to the teachings of the invention without departing from the essential scope thereof. Accordingly, it is intended that the invention not be limited to the particular embodiment disclosed as the best mode contemplated for carrying out this invention, but that the invention be construed as including all embodiments falling within the scope of the appended claims.

Claims (11)

A cosmetic composition for anti-aging and skin wrinkle improvement, comprising an extract of Pleurotus cystidiosus mycelium or fruiting body at a concentration of 0.2 to 0.4% (w / v). delete The method of claim 1,
The deposit number of the abalone strain is IUM01232 (accession number of mushroom strain and DNA bank) cosmetic composition, characterized in that. A pharmaceutical composition for anti-aging and skin wrinkle improvement, comprising an extract of Pleurotus cystidiosus mycelium or fruiting body at a concentration of 0.2 to 0.4% (w / v). 5. The method of claim 4,
The accession number of the abalone strain is IUM01232 (accession number of mushroom strain and DNA bank) pharmaceutical composition, characterized in that. Pleurotus cystidiosus A health functional food for anti-aging and skin wrinkle improvement containing extracts of mycelium or fruiting bodies as an active ingredient. delete delete delete delete A food additive for anti-aging and skin wrinkle improvement containing an extract of Pleurotus cystidiosus mycelium or fruiting body at a concentration of 0.2 to 0.4% (w / v).