KR101292734B1 - Method for prepairing chunggukjang enriched mineral using glassworts - Google Patents

Method for prepairing chunggukjang enriched mineral using glassworts Download PDF

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KR101292734B1
KR101292734B1 KR1020110047598A KR20110047598A KR101292734B1 KR 101292734 B1 KR101292734 B1 KR 101292734B1 KR 1020110047598 A KR1020110047598 A KR 1020110047598A KR 20110047598 A KR20110047598 A KR 20110047598A KR 101292734 B1 KR101292734 B1 KR 101292734B1
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cheonggukjang
bark
tung
soybeans
extract
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KR20120129375A (en
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이영재
박정욱
박인배
신궁원
한미영
조영철
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전라남도
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    • AHUMAN NECESSITIES
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    • A23L11/00Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/50Fermented pulses or legumes; Fermentation of pulses or legumes based on the addition of microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/70Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • A23L2/72Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by filtration
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/16Inorganic salts, minerals or trace elements
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    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/51Concentration
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    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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    • A23V2250/156Mineral combination
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    • A23V2250/00Food ingredients
    • A23V2250/20Natural extracts
    • A23V2250/21Plant extracts
    • AHUMAN NECESSITIES
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    • A23V2300/00Processes
    • A23V2300/14Extraction
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    • A23V2300/24Heat, thermal treatment
    • AHUMAN NECESSITIES
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    • A23V2300/00Processes
    • A23V2300/50Concentrating, enriching or enhancing in functional factors
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07Bacillus
    • C12R2001/125Bacillus subtilis ; Hay bacillus; Grass bacillus

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Abstract

본 발명은 퉁퉁마디를 이용한 미네랄 강화 청국장의 제조 방법에 관한 것으로, 보다 상세하게는 미네랄을 다량 함유한 퉁퉁마디를 추출액에 콩을 침지하여 미네랄과 영양성분을 콩에 흡착시킨 다음 발효숙성시킴으로써 미네랄을 다량함유할 뿐만 아니라 콩의 발효도 촉진하여 맛과 영양을 향상시킬 수 있는 퉁퉁마디를 이용한 미네랄 강화 청국장의 제조 방법에 관한 것이다.
본 발명에 따른 퉁퉁마디를 이용한 미네랄 강화 청국장의 제조 방법은, 퉁퉁마디를 열수 추출하여 퉁퉁마디 추출액을 제조하는 단계와, 콩을 퉁퉁마디 추출액에 침지시키고 증자하여 증자 콩을 만든 후, 그 증자 콩에 고초균(Bacillus subtillis)을 접종하고 발효시켜 청국장을 제조하는 단계를 포함하는 것을 특징으로 한다.
The present invention relates to a method for producing mineral-enriched Cheonggukjang using tung bark, and more specifically, dung tung bark containing a large amount of minerals by immersing soybeans in the extract to adsorb minerals and nutrients to soybeans, and then fermenting and aging the minerals. The present invention relates to a method for preparing mineral-enriched Cheonggukjang using a bark which can not only contain a large amount but also promote fermentation of soybeans to improve taste and nutrition.
According to the present invention, a method for preparing mineral-enriched Cheonggukjang using tung bark comprises the steps of preparing hot tung bark extract by hydrothermally extracting tung bark, and dipping and soaking the soybean in the tung bark extract to make steamed beans. Bacillus subtilis ( Bacillus subtillis ) to inoculate and fermentation is characterized in that it comprises the step of preparing the cheonggukjang.

Description

퉁퉁마디를 이용한 미네랄 강화 청국장의 제조 방법{METHOD FOR PREPAIRING CHUNGGUKJANG ENRICHED MINERAL USING GLASSWORTS}METHOD FOR PREPAIRING CHUNGGUKJANG ENRICHED MINERAL USING GLASSWORTS}

본 발명은 퉁퉁마디를 이용한 미네랄 강화 청국장의 제조 방법에 관한 것으로, 보다 상세하게는 미네랄을 다량 함유한 퉁퉁마디를 추출액에 콩을 침지하여 미네랄과 영양성분을 콩에 흡착시킨 다음 발효숙성시킴으로써 미네랄을 다량함유할 뿐만 아니라 콩의 발효도 촉진하여 맛과 영양을 향상시킬 수 있는 퉁퉁마디를 이용한 미네랄 강화 청국장의 제조 방법에 관한 것이다.The present invention relates to a method for producing mineral-enriched Cheonggukjang using tung bark, and more specifically, dung tung bark containing a large amount of minerals by immersing soybeans in the extract to adsorb minerals and nutrients to soybeans, and then fermenting and aging the minerals. The present invention relates to a method for preparing mineral-enriched Cheonggukjang using a bark which can not only contain a large amount but also promote fermentation of soybeans to improve taste and nutrition.

청국장은 콩을 이용한 전통발효식품으로 볏짚 위에 찐 콩을 담아 40℃에서 2~3일 발효 숙성시키면 고초균(Bacillus subtillis)이 생산하는 효소에 의하여 단백질과 당질이 분해되어 levan form fructan과 polyglutamate로 구성된 끈끈한 점질물이 생성되면서 특유한 냄새와 고유한 맛을 띤다. 또한 콩에서 기인된 isoflavone, phytic acid, saponin, trypsin inhibitor, tocopherol, 불포화지방산, 식이섬유, 올리고당 등의 각종 생리활성물질과 항산화물질 및 혈전용해효소를 다량 함유하고 있기 때문에 기능성 식품으로서의 중요성이 강조되고 있다.Cheonggukjang is a traditional fermented food using soybeans. Steamed soybeans on rice straw are fermented and fermented at 40 ℃ for 2 ~ 3 days. Bacillus The enzymes produced by subtillis ) decompose proteins and sugars to produce sticky viscous materials consisting of levan form fructan and polyglutamate, which have a unique odor and unique taste. In addition, since it contains a large amount of various bioactive substances such as isoflavone, phytic acid, saponin, trypsin inhibitor, tocopherol, unsaturated fatty acid, dietary fiber, oligosaccharide, and antioxidants and thrombolytic enzymes derived from soybeans, the importance as a functional food is emphasized. have.

퉁퉁마디(Salicornia herbacea, glasswort)는 명아주과(Chenopodiaceae)에 속하는 일년생 초본으로 염류 농도가 높은 곳에서 자라기 때문에 삼투압을 견디기 위해 식물체 내에 육상식물보다 다량의 염분을 저장 및 축적하고 있으며, 고농도의 염스트레스에 내성을 가지기 위해 betaine과 같은 양쪽성 물질(zwitterionic compound)을 함유하여 고 삼투압 환경에 적응하는 것으로 알려져 있다. 또한, 퉁퉁마디는 마그네슘, 칼슘, 철 그리고 칼륨 등의 미네랄과 식이섬유를 다량 함유하고 있을 뿐만 아니라 아미노산과 지방산이 풍부하여 숙변, 변비, 소화불량, 위장병, 간염 및 신장병의 치료제로 한방 및 민간에서 널리 사용하여 왔다. 퉁퉁마디는 항산화작용, 항당뇨, 항고지혈증, 효소적 가수분해물의 혈당 강하 및 혈청 지질 개선 등의 기능이 알려져 있다. Salicornia herbacea , glasswort) is an annual herb belonging to the Chenopodiaceae, and grows in high salt concentrations, so it saves and accumulates more salts than plants in the plants to withstand osmotic pressure, and to withstand high concentrations of salt stress. It is known to contain zwitterionic compounds such as betaine to adapt to high osmotic environments. In addition, it contains a lot of minerals and dietary fiber, such as magnesium, calcium, iron and potassium, and is rich in amino acids and fatty acids, and is used in herbal medicine and civilian medicine as a treatment for stool, constipation, indigestion, gastrointestinal diseases, hepatitis and kidney disease. It has been widely used. Kung bark is known for its functions such as antioxidant activity, anti-diabetic, anti-hyperlipidemia, hypoglycemia of enzymatic hydrolysates and improvement of serum lipids.

이와 같이 인체에 유용한 성분이 다량 함유되어 있는 퉁퉁마디를 이용하여 다양한 기능의 건강식품으로 개발이 되고 있다. 예를 들면 대한민국 등록특허공보 제10-0587399호는 함초에 현미식초를 첨가하여 간장과 된장의 제조방법에 관한 것이고, 대한민국 등록특허공보 제10-0882003호는 청국장, 함초 및 표고버섯으로 이루어진 기능성 건강식품의 제조방법에 관한 것이 있다.As such, it is being developed as a health food with a variety of functions by using a lumpy grain containing a large amount of useful components for the human body. For example, Republic of Korea Patent Publication No. 10-0587399 relates to the manufacturing method of soy sauce and miso by adding brown rice vinegar to the hamcho, Republic of Korea Patent Publication No. 10-0882003 is functional health consisting of Cheonggukjang, hamcho and shiitake mushroom There is a method of manufacturing food.

그러나 지금까지 알려진 청국장 제조법에는 콩을 퉁퉁마디 추출물의 유용성분들에 침지 흡착하여 발효를 촉진시켜서 청국장을 제조한 사례는 없으며, 단순하게 분말을 첨가하는 수준에 그치고 있어 여러 가지 유익한 성분에 대한 효능을 최대한 발휘할 수 없었다.However, no known cheonggukjang recipe has been prepared by immersing and soaking soybeans in useful components of the bark extracts to promote fermentation, and simply adding powders to maximize the efficacy of various beneficial ingredients. Could not be exercised.

본 발명은 상기와 같은 점을 인식하여 안출된 것으로 본 발명의 목적은 미네랄을 다량 함유한 퉁퉁마디를 추출액에 콩을 침지하여 미네랄과 영양성분을 콩에 흡착시킨 다음 발효숙성시킴으로써 미네랄을 다량함유할 뿐만 아니라 콩의 발효도 촉진하여 맛과 영양을 향상시킬 수 있는 퉁퉁마디를 이용한 미네랄 강화 청국장의 제조 방법을 제공하기 위한 것이다.The present invention was conceived by recognizing the above-mentioned point is an object of the present invention is to immerse the soybeans in extracts containing a large amount of minerals soaking the minerals and nutrients in soybeans and then fermenting and ripening to contain a large amount of minerals In addition, to promote the fermentation of soybeans to improve the taste and nutrition is to provide a method of producing mineral-enriched Cheonggukjang using a bark.

상기와 같은 목적을 달성하기 위하여 본 발명에 따른 퉁퉁마디를 이용한 미네랄 강화 청국장의 제조 방법은, 퉁퉁마디를 열수 추출하여 퉁퉁마디 추출액을 제조하는 단계와, 콩을 퉁퉁마디 추출액에 침지시키고 증자하여 증자 콩을 만든 후, 그 증자 콩에 고초균(Bacillus subtillis)을 접종하고 발효시켜 청국장을 제조하는 단계를 포함하는 것을 특징으로 한다.In order to achieve the above object, the manufacturing method of mineral-tung Cheonggukjang using tung bark according to the present invention comprises the steps of preparing the tung bark extract by hydrothermally extracting the tung bark, and soaking the beans in the tung bark extract to increase the steam. After making soybeans, Bacillus subtillis ) is characterized in that it comprises the step of inoculating and fermenting to prepare the Cheonggukjang.

또한, 본 발명에 따른 퉁퉁마디를 이용한 미네랄 강화 청국장의 제조 방법은, 상기 퉁퉁마디 추출액을 제조하는 단계는, 건조시킨 퉁퉁마디를 분쇄기로 분말화시키는 단계와, 상기 퉁퉁마디 분말 1Kg 중량부에 정제수 5L 부피부를 가하여 98~100℃에서 2~4시간동안 열수추출한 다음 여과포로 여과하고 감압하에 농축하여 염도 4%의 퉁퉁마디 추출액을 제조하는 단계를 포함하고, 상기 청국장을 제조하는 단계는, 콩 1Kg 중량부를 상기 퉁퉁마디 추출액 2L 부피부에 12~18시간 침지시키고, 고압살균솥으로 가열처리하여 증자 콩을 만드는 단계와, 증자된 콩에 고초균(Bacillus subtillis)으로 접종하고 37~40℃에서 24~72시간 발효숙성시키는 단계를 포함하는 것을 특징으로 한다.In addition, the method of producing mineral-tung Cheonggukjang using the bark according to the present invention, the step of producing the bark extract, the step of powdering the dried bark with a grinder, and 1Kg weight of the bark powder 5L purified water Adding hot water at a temperature of 98-100 ° C. for 2-4 hours, filtering with a filter cloth, and concentrating under reduced pressure to prepare a Tung-Madi extract of salinity of 4%. 12 parts by weight in 2L volume of the sulphate extract, 12-18 hours immersed in a high-pressure sterilization cooker to make cooked beans, and Bacillus Bacillus in the cooked beans subtillis ) and characterized in that it comprises the step of fermentation at 37 ~ 40 ℃ 24 ~ 72 hours fermentation.

상기와 같은 구성에 의하여 본 발명에 따른 퉁퉁마디를 이용한 미네랄 강화 청국장의 제조 방법은 미네랄을 다량 함유한 퉁퉁마디를 추출액에 콩을 침지하여 미네랄과 영양성분을 콩에 흡착시킨 다음 발효숙성시킴으로써 미네랄을 다량함유할 뿐만 아니라 콩의 발효도 촉진하여 맛과 영양을 향상시킬 수 있는 장점을 갖는다.The method for producing mineral-enriched Cheonggukjang using the bark according to the present invention by the configuration as described above is to immerse the soybeans in the extract with a lot of minerals soaking the soybeans in the extract and adsorb the minerals and nutrients to the beans and then ferment and mature the minerals. Not only does it contain a large amount, but also promotes fermentation of soybeans, which has the advantage of improving taste and nutrition.

도 1은 본 발명의 일실시예에 따른 퉁퉁마디를 이용한 미네랄 강화 청국장의 제조 방법을 도시한 흐름도1 is a flow chart showing a method of producing mineral-enriched Cheonggukjang using a bark according to an embodiment of the present invention

이하에서는 도면 및 실시예를 참조하여 본 발명에 따른 퉁퉁마디를 이용한 미네랄 강화 청국장의 제조 방법을 보다 상세하게 설명하기로 한다.Hereinafter, with reference to the drawings and examples will be described in more detail the manufacturing method of the mineral-enhanced Cheonggukjang using the bark according to the present invention.

도 1은 본 발명의 일실시예에 따른 퉁퉁마디 미네랄 강화 청국장의 제조 방법을 도시한 흐름도로서, 도면을 참조하면 본 발명의 일실시예에 따른 퉁퉁마디 미네랄 강화 청국장의 제조 방법은 다음과 같다.1 is a flow chart illustrating a manufacturing method of tungtong mineral reinforced Cheonggukjang according to an embodiment of the present invention. Referring to the drawings, the manufacturing method of the Tungmad mineral reinforced Cheonggukjang according to an embodiment of the present invention is as follows.

(1) 퉁퉁마디 추출액 제조(1) Preparation of Squash Extract

채취한 퉁퉁마디를 세척하여 뿌리와 이물질을 제거하고 건조시킨 퉁퉁마디를 분쇄기로 분말화한다. 상기 퉁퉁마디 분말에 정제수(정제수 5L 부피:퉁퉁마디 1Kg 중량)를 가하여 98~100℃에서 2~4시간동안 열수추출한 다음 여과포로 여과하고 감압하에 농축하여 염도 4%의 퉁퉁마디 추출액을 제조한다.The collected burrs are washed to remove roots and foreign substances, and the dried burrs are powdered with a grinder. Purified water (5L volume of purified water: 1Kg weight of kung bark) was added to the kung bark powder, followed by hot water extraction at 98 ~ 100 ° C. for 2 ~ 4 hours, filtered through a filter cloth, and concentrated under reduced pressure to prepare a kung bark extract having a salinity of 4%.

(2) 퉁퉁마디 미네랄 강화 청국장의 제조(2) production of tungdi mineral reinforcing Cheonggukjang

정선하고 세척한 콩을 상기 퉁퉁마디 추출액(퉁퉁마디 추출액 2L 부피:콩 1Kg 중량)에 12~18시간 침지시키고, 고압살균솥으로 가열처리하여 증자 콩을 만든 다음 증자된 콩에 고초균(Bacillus subtillis)으로 접종하고 37~40℃에서 24~72시간 발효숙성시켜 생청국장을 제조한다.Choice and the bean cleaning the Salicornia extract (Salicornia extract 2L volume: beans 1Kg by weight) was immersed in 12 ~ 18 hours, to a heat treatment by autoclaving of the cooker and then steamed beans made of steamed beans Bacillus subtilis (Bacillus inoculate with subtillis ) and fermentation aging for 24 to 72 hours at 37 ~ 40 ℃ to prepare the saengcheonggukjang.

재래의 방법으로는 고초균이 많이 번식해 있는 볏짚을 갈아 놓은 곳에 증자한 콩을 놓거나 볏짚 토막을 콩에 꽂아 접종할 수도 있고 통상의 방법으로 고초균을 별도로 배양하여 증식시킨 후 멸균생리식염수로 희석하여 접종할 수도 있다. 청국장 발효에 필요한 고초균(Bacillus subtillis)은 탄소원으로 포도당이나 자당 및 과당 등과 질소원으로 글루탐산, 아르기닌, 아스파라긴산, 프롤린 등의 아미노산과 비타민, 미네랄 등을 그 생육에 필요한 영양원으로 필요로 한다. 콩은 상기의 영양원을 다량함유하고 있으므로 고초균의 생육을 위한 좋은 배지가 된다.Conventional methods can be inoculated by placing the soybeans in the place where rice straw, which has many breeding varieties of rice, or by inserting rice straw into soybeans, or inoculating them by diluting them with sterile physiological saline. You may. Bacillus for Fermenting Cheonggukjang subtillis ) is a carbon source of glucose, sucrose, fructose and nitrogen as amino acids, such as glutamic acid, arginine, aspartic acid, proline, amino acids, vitamins, minerals, etc. are required for its growth. Soy is a good medium for the growth of Bacillus subtilis because it contains a lot of the above nutrients.

특히, 본 발명은 퉁퉁마디에 함유된 당류와 아미노산, 특히 풍부한 다양한 미네랄에 의해 고초균의 발아 및 세포분열을 더욱 활성화되어 청국장의 발효기간을 단축시킬 수 있을 뿐만 아니라 현대인에게 부족하기 쉬운 미네랄을 강화시킨 청국장을 얻을 수 있다.In particular, the present invention is activated by germination and cell division of Bacillus subtilis by the sugars and amino acids, especially rich in various minerals contained in the bark can not only shorten the fermentation period of Cheonggukjang but also strengthen the minerals that are easily lacking in modern people Cheonggukjang can be obtained.

(3) 퉁퉁마디 미네랄 강화 청국장 분말 제조(3) Tung Kyun Mineral Strengthened Cheonggukjang Powder Manufacturing

상기 발효된 생청국장을 건조하여 분쇄기로 100~150 mesh로 분말화하여 생청국장 분말을 제조한다. 상기와 같이 제조된 생청국장 분말은 분말상태 또는 캅셀에 충진하거나, 환이나 과립 등으로 성형하여 가공하여 휴대가 용이하고 먹고 싶을 때 쉽게 먹을 수 있도록 하는 것이 바람직하다.
The fermented saengcheonggukjang is dried to powder 100 ~ 150 mesh with a grinder to prepare a saengcheonggukjang powder. The raw cheonggukjang powder prepared as described above is preferably filled in a powder state or capsule, or molded and processed into a pill or granule so as to be portable and easily eaten when desired.

상기와 같이 제조된 본 발명의 일실시예에 따라 제조된 퉁퉁마디를 이용한 미네랄 강화 청국장 및 청국장 분말은 퉁퉁마디의 미네랄이 강화되어 청국장 특유의 냄새를 저감시키고, 다양하고 풍부한 영양소가 상호작용하여 기호도를 증가시킨다.
Mineral-enhanced Cheonggukjang and Cheonggukjang powder using the Tung Kung Kung prepared according to an embodiment of the present invention prepared as described above to enhance the minerals of Kung Kung Kung decrease the peculiar odor of Cheong Kuk Chang, and various rich nutrients interact with each other. To increase.

이하에서는 실시예를 참조하여 본 발명에 따른 퉁퉁마디를 이용한 미네랄 강화 청국장의 제조방법을 보다 상세하게 설명하기로 한다.Hereinafter, with reference to the embodiment will be described in more detail the manufacturing method of the mineral strengthened Cheonggukjang using the bark according to the present invention.

<실시예 1 : 퉁퉁마디 추출액의 제조><Example 1: Preparation of spurs extract>

채취한 퉁퉁마디를 세척하여 뿌리와 이물질을 제거하고 건조시킨 퉁퉁마디를 분쇄기로 분말화한다.The collected burrs are washed to remove roots and foreign substances, and the dried burrs are powdered with a grinder.

상기 퉁퉁마디 분말 2Kg에 정제수 10L를 가하여 100℃에서 3시간동안 열수추출한 다음 여과포로 여과하여 감압하에 농축하여 염도 4%의 퉁퉁마디 추출액을 제조한다.
10 L of purified water was added to 2 Kg of the lumped powder, extracted with hot water at 100 ° C. for 3 hours, filtered through a filter cloth, and concentrated under reduced pressure to prepare a lumped extract of 4% salinity.

<실시예 2 : 퉁퉁마디 미네랄 강화 청국장의 제조><Example 2: Preparation of Tung Bark Mineral Enhanced Cheonggukjang>

정선하고 세척한 콩 1Kg에 퉁퉁마디 추출액을 2L에 12시간 침지시키고, 고압살균솥로 30분간 가열처리하여 증자 콩을 만든 다음 증자된 콩에 고초균(Bacillus subtillis)으로 접종하고 37℃, 48시간 발효숙성시켜 생청국장을 제조하였다.
1Kg of selected and washed soybeans were dipped into 2L of tung bark extract for 12 hours, heated for 30 minutes with a autoclave to make steamed beans, and then inoculated with Bacillus subtillis in the cooked beans and fermented at 37 ℃ for 48 hours. Aged and prepared soybean soup.

<실시예 3 : 퉁퉁마디 미네랄 강화 청국장 분말의 제조>Example 3 Preparation of Sung Kite Mineral-Reinforced Cheonggukjang Powder

상기 발효된 생청국장을 60℃로 건조하여 분쇄기를 이용하여 100 mesh이하로 분말화하여 생청국장 분말을 제조하였다.
The fermented saengcheonggukjang was dried to 60 ℃ and powdered to less than 100 mesh using a grinder to prepare a saengcheonggukjang powder.

<비교예 1 : 퉁퉁마디 무첨가 일반 청국장의 제조><Comparative Example 1: Manufacture of General Cheonggukjang without additives>

퉁퉁마디 미네랄 강화 청국장과 비교하기 위해 퉁퉁마디 무첨가 일반 청국장의 제조는 콩 1Kg을 세척하고 2L의 정제수에 12시간 침지하고 물 빼기를 한다. 고압살균솥에서 121℃, 30분간 증자한 후 콩의 품온을 30℃가 될 때까지 냉각시키고 고초균(Bacillus subtillis)으로 접종하고 37℃, 48시간 발효숙성시켜 일반 청국장을 제조하였다.
To compare with the Tung Kyun mineral-enriched Cheonggukjang, the manufacture of Tung Kyun without Tung Kyun Kung is washed with 1Kg of soybeans, soaked in 2L of purified water for 12 hours and drained. After steaming for 30 minutes at 121 ℃ in a autoclave, the bean's temperature is cooled to 30 ℃ and Bacillus subtillis ) was fermented and aged at 37 ° C. for 48 hours to prepare a general Cheonggukjang.

<시험 1 : 퉁퉁마디의 성분분석><Test 1: Component analysis of spurs>

상기 실시예1에 사용된 퉁퉁마디의 성분분석을 분석한 결과를 하기 표1에 정리하였다. 표1에 따르면 퉁퉁마디는 기능성 성분을 많이 함유하고 있는 소재임을 확인할 수 있다.Table 1 summarizes the results of analyzing the component analysis of the bark used in Example 1. According to Table 1 it can be confirmed that the bump is a material containing a lot of functional ingredients.

항목Item 함량content 항목Item 함량content 회분(%)Ashes (%) 32.3232.32 아스파르트산(mg/100g)Aspartic Acid (mg / 100g) 369.75369.75 조지방(%)Crude fat (%) 2.182.18 트레오닌(mg/100g)Threonine (mg / 100g) 164.57164.57 조단백질(%)Crude protein (%) 6.766.76 세린(mg/100g)Serine (mg / 100g) 206.28206.28 탄수화물(%)carbohydrate(%) 58.7458.74 글루탐산(mg/100g)Glutamic Acid (mg / 100g) 466.58466.58 폴리페놀(mg/100g)Polyphenol (mg / 100g) 1435.131435.13 글리신(mg/100g)Glycine (mg / 100g) 212.16212.16 플라보노이드(mg/100g)Flavonoids (mg / 100g) 556.95556.95 알라닌(mg/100g)Alanine (mg / 100g) 212.42212.42 비타민 C(mg/100g)Vitamin C (mg / 100g) 133.16133.16 발린(mg/100g)Valine (mg / 100g) 227.13227.13 베타인(mg/mL)Betaine (mg / mL) 1.601.60 메치오닌(mg/100g)Methionine (mg / 100g) 42.2642.26 아연(mg/kg)Zinc (mg / kg) 27.8227.82 이소류신(mg/100g)Isoleucine (mg / 100g) 183.09183.09 구리(mg/kg)Copper (mg / kg) 21.3421.34 류신(mg/100g)Leucine (mg / 100g) 322.97322.97 칼슘(mg/kg)Calcium (mg / kg) 8520.888520.88 티로신(mg/100g)Tyrosine (mg / 100g) 177.30177.30 철(mg/kg)Iron (mg / kg) 282.83282.83 페닐알라닌(mg/100g)Phenylalanine (mg / 100g) 203.37203.37 망간(mg/kg)Manganese (mg / kg) 75.2775.27 히스티딘(mg/100g)Histidine (mg / 100g) 104.35104.35 나트륨(mg/kg)Sodium (mg / kg) 92898.0692898.06 리신(mg/100g)Lysine (mg / 100g) 230.52230.52 칼륨(mg/kg)Potassium (mg / kg) 11812.7611812.76 아르기닌(mg/100g)Arginine (mg / 100g) 193.49193.49 마그네슘(mg/kg)Magnesium (mg / kg) 7959.677959.67 프로린(mg/100g)Proline (mg / 100g) 173.59173.59

<시험 2 : 청국장의 품질특성><Test 2: Quality Characteristics of Cheonggukjang>

상기 실시예2와 비교예1에서 제조한 퉁퉁마디 미네랄 청국장과 일반 청국장의 품질특성을 분석한 결과를 표2에 나타내었다. 표2를 참조하면, 염도는 일반 청국장은 0.3%였고, 퉁퉁마디 미네랄 청국장의 2.2%로 높게 나타났다. pH는 큰 차이를 보이지 않았고, 아미노태질소는 일반 청국장이 퉁퉁마디 청국장에 비해 조금 높았다.Table 2 shows the results of analyzing the quality characteristics of the Tung bark mineral Cheonggukjang and General Cheonggukjang prepared in Example 2 and Comparative Example 1. Referring to Table 2, the salinity was 0.3% in the general Cheonggukjang and 2.2% of the mineral burial. The pH did not show a big difference, and amino nitrogen was a little higher than normal Cheonggukjang.

시료명Name of sample 염도(%)Salinity (%) pHpH 아미노태질소(mg%)Amino nitrogen (mg%) 일반 청국장General Cheonggukjang 0.30.3 7.27.2 698.5698.5 퉁퉁마디 청국장Bongmad Cheonggukjang 2.22.2 7.07.0 687.4687.4

<시험 3 : 청국장 미네랄 분석><Test 3: Cheonggukjang Mineral Analysis>

상기 실시예2와 비교예1에서 제조한 퉁퉁마디 미네랄 청국장과 일반 청국장의 미네랄을 ICP-OES로 분석한 결과를 표3에 나타내었다.Table 3 shows the results of ICP-OES analysis on the minerals of Tung Kyun mineral and Cheonggukjang prepared in Example 2 and Comparative Example 1.

표3을 참조하면, 일반 청국장에 비해 퉁퉁마디 청국장에서 미네랄 함량이 높게 나타났다.Referring to Table 3, it was found that the mineral content was higher in Cheonggukjang, which was compared to the general Cheonggukjang.

성분(mg/kg)
시료명
Ingredient (mg / kg)
Name of sample
아연zinc sign 칼슘calcium iron 망간manganese 나트륨salt 칼륨potassium 마그네슘magnesium
일반 청국장General Cheonggukjang 14.514.5 3,1893,189 1,3111,311 31.031.0 9.59.5 3434 5,6215,621 981981 퉁퉁마디 미네랄 청국장Tungmadul Mineral Cheonggukjang 20.820.8 4,0734,073 1,2651,265 20.720.7 22.522.5 10,97410,974 6,8126,812 2,1662,166

<시험 4 : 청국장의 항산화활성><Test 4: Antioxidant Activity of Cheonggukjang>

상기 실시예2와 비교예1에서 제조한 퉁퉁마디 미네랄 청국장과 일반 청국장의 항산화활성을 측정한 결과를 표4에 나타내었다.Table 4 shows the results of measuring the antioxidant activity of the Tung bark mineral Cheonggukjang and General Cheonggukjang prepared in Example 2 and Comparative Example 1.

표4를 참조하면, DPPH(1,1-diphenyl-2-picrylhydrazyl) radical 소거능으로 측정한 항산화활성은 일반 청국장에 비해 퉁퉁마디 미네랄 청국장이 퉁퉁마디 첨가의 영향으로 항산화활성이 높은 것으로 나타났다.Referring to Table 4, the antioxidant activity measured by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity was higher than that of normal Cheonggukjang.

시료명Name of sample 항산화활성(%)Antioxidant activity (%) 일반 청국장General Cheonggukjang 23.723.7 퉁퉁마디 미네랄 청국장Tungmadul Mineral Cheonggukjang 52.352.3

<시험 5 : 청국장의 Angiotensin converting enzyme(ACE) 저해효과><Test 5: Cheonggukjang's Angiotensin converting enzyme (ACE) Inhibitory Effect>

상기 실시예2와 비교예1에서 제조한 퉁퉁마디 미네랄 청국장과 일반 청국장의 Angiotensin converting enzyme(ACE) 저해효과를 측정한 결과를 표 5에 나타내었다.Table 5 shows the results of measuring the inhibitory effect of Angiotensin converting enzyme (ACE) on the soybean curd and Chungcheongjang prepared in Example 2 and Comparative Example 1.

표5를 참조하면, 일반 청국장의 ACE 저해효과는 85.4%였고, 퉁퉁마디 미네랄 청국장은 92.2%로 나타났으며 퉁퉁마디의 유효성분이 ACE 저해효과를 상승시킨 것으로 보인다.Referring to Table 5, ACE inhibitory effect was 85.4% in general Cheonggukjang, 92.2% of mineral burial in Soongkungjang, and the active ingredient of Kungjang increased the ACE inhibitory effect.

시료명Name of sample Angiotensin converting enzyme 저해효과(%)Inhibitory effect of angiotensin converting enzyme (%) 일반 청국장General Cheonggukjang 85.485.4 퉁퉁마디 미네랄 청국장Tungmadul Mineral Cheonggukjang 92.292.2

이상의 설명은 본 발명의 기술 사상을 예시적으로 설명한 것으로 본 발명에 속하는 기술분야는 통상의 지식을 가진 자라면 본 발명의 본질적인 특성에서 벗어나지 않는 범위내에서 다양한 수정 및 변형이 가능할 것이다. 따라서 본 발명에 개시된 실시예들은 본 발명의 기술사상을 한정하기 위한 것이 아니라 설명하기 위한 것이고, 이러한 실시예에 의해 본 발명의 기술사상의 범위가 한정되는 것은 아니다. 본 발명의 보호범위는 아래의 청구범위에 의해 해석되어야 하며, 그와 동등한 범위내에 있는 모든 기술사상은 본 발명의 권리범위에 포함되는 것으로 해석되어야 할 것이다.The above description exemplarily illustrates the technical idea of the present invention, and those skilled in the art may make various modifications and changes without departing from the essential characteristics of the present invention. Therefore, the embodiments disclosed in the present invention are not intended to limit the technical spirit of the present invention but to explain, and the scope of the technical spirit of the present invention is not limited by these embodiments. The scope of protection of the present invention should be interpreted by the following claims, and all technical ideas within the scope equivalent thereto should be construed as being included in the scope of the present invention.

Claims (2)

퉁퉁마디를 열수 추출하여 퉁퉁마디 추출액을 제조하는 단계와,
콩을 퉁퉁마디 추출액에 침지시키고 증자하여 증자 콩을 만든 후, 그 증자 콩에 고초균(Bacillus subtillis)을 접종하고 발효시켜 청국장을 제조하는 단계를 포함하되,
상기 퉁퉁마디 추출액을 제조하는 단계는, 건조시킨 퉁퉁마디를 분쇄기로 분말화시키는 단계와, 상기 퉁퉁마디 분말 1Kg 중량부에 정제수 5L 부피부를 가하여 98~100℃에서 2~4시간동안 열수추출한 다음 여과포로 여과하고 감압하에 농축하여 염도 4%의 퉁퉁마디 추출액을 제조하는 단계를 포함하고,
상기 청국장을 제조하는 단계는, 콩 1Kg 중량부를 상기 퉁퉁마디 추출액 2L 부피부에 12~18시간 침지시키고, 고압살균솥으로 가열처리하여 증자 콩을 만드는 단계와, 증자된 콩에 고초균(Bacillus subtillis)으로 접종하고 37~40℃에서 24~72시간 발효숙성시키는 단계를 포함하는 것을 특징으로 하는 퉁퉁마디를 이용한 미네랄 강화 청국장의 제조 방법.
Hydrothermally extracting the tung nodes to prepare a tung bark extract;
After dipping the soybeans in the soybean bark extract and cooking the cooked soybeans , and inoculating and fermenting the Bacillus subtillis in the cooked soybeans, the step of preparing the Cheonggukjang,
The step of preparing the tung bark extract, powdering the dried tung bark with a pulverizer, by adding 5L volume of purified water to 1Kg weight of the kung bark powder, hot water extraction for 2-4 hours at 98 ~ 100 ℃ and then filter cloth Filtering to and concentrating under reduced pressure to prepare a stalk extract of 4% salinity,
The step of preparing the Cheonggukjang, soaking 1Kg parts of soybeans in 2L volume of the sulphate extract 12 to 18 hours, heat-processed with a high-pressure sterilizer to make a steamed beans, Bacillus subtillis Inoculation to and fermentation at 37 ~ 40 ℃ 24 ~ 72 hours fermentation aging step, characterized in that the manufacturing method of mineral fortified Cheonggukjang using.
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KR20100107862A (en) * 2009-03-27 2010-10-06 바이오샐리코영농조합법인 Process for preparing salt and soybean paste derived from salicornia herbacea

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KR20100107862A (en) * 2009-03-27 2010-10-06 바이오샐리코영농조합법인 Process for preparing salt and soybean paste derived from salicornia herbacea

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