KR101253259B1 - Sterilization composition and sterilization method using the same - Google Patents

Abstract

The present invention relates to a disinfectant composition and a sterilization method using the same. More specifically, the sterilization effect including the polyalkyleneguanidine salt and sodium pyrithione is excellent in the sterilization effect and the fast-acting effect and the sustainability is improved. The present invention relates to a disinfectant composition that enables sterilization of bacteria, fungi and algae over a sterilization method and a sterilization method using the same.

Fungicides, polyhexamethylene guanidine salts, pyrithione sodium, preservatives, bactericidal, sustained sterilization, bacteria, fungi

Description

Fungicide composition and sterilization method using same {STERILIZATION COMPOSITION AND STERILIZATION METHOD USING THE SAME}

1 is a graph showing the activity of microorganisms over time, obtained in accordance with an embodiment of the present invention.

The present invention relates to a disinfectant composition and a sterilization method using the same. More particularly, the sterilization composition and sterilization method using the same further improve the sterilization effect, fast-acting effect and sustainability, thereby enabling the sterilization of bacteria, mold and algae throughout the industry, including pulp production and various water treatment devices. It relates to a disinfectant composition and a sterilization method using the same.

Various emulsions are used in industrial water, and microorganisms such as bacteria, fungi, algae, etc., are grown as nutrients to inhibit the efficient operation of industrial processes. Microorganisms propagate organic matter contained in industrial water as a nutrient source, secrete polysaccharides, and various organic and inorganic substances are combined with the secreted polysaccharides to produce a viscous mass called slime.

In particular, cellulose, hemicellulose and organic matter present in the white water of the papermaking process become nutrients rich in microorganisms, and slime is formed in the weakened fluid flow. Such slime formation leads to direct and indirect losses such as loss of manufacturing time due to deterioration of pulp quality, paper cutting, and deterioration of equipment efficiency.

In addition, in the case of an industrial cooling tower, as the cooling water facilities collect a lot of water, or the microorganism grows actively in the place where the water is recycled, causing fouling (Fouling) phenomenon. In addition, it not only lowers the heat transfer efficiency, but also corrodes the metal and causes erosion of the wood part.

Bacteria are unicellular prokaryotes that can multiply by breaking down various types of organics. Some of them secrete polysaccharides to the outside to form a biofilm, which causes microbiologically induced corrosion.

Fungi are eukaryotes that can multiply and disintegrate various types of organic matter, similar to bacteria, and the types of cellulase that dissociate the fibers of wood parts such as cooling towers, causing discoloration and rot. Becomes

Algae, another microorganism, multiplies through photosynthesis even under light, air and small amounts of minerals. Carbohydrates produced by algae are used as nutrients for other microbes, such as bacteria and fungi, to accelerate fouling. In particular, in the case of sun exposure, such as cooling water facilities, fouling due to the growth of algae is intensified, causing clogging of the water pipe and lowering the heat transfer efficiency. In addition, oxygen is generated to oxidize the metal surface, and upon death, it causes local corrosion to promote corrosion at the hole of the metal.

In order to kill such microorganisms, mold, algae, or the like to prevent surface adhesion to metals, various fungicides have been developed, and such fungicides are generally classified into oxidizing fungicides and non-oxidizing fungicides.

Halogen compounds, such as chlorine and bromine, are mainly used as oxidizing fungicides, and they are widely used in terms of economy because of their high oxidizing power and low cost. However, oxidizing fungicides cause wood erosion and metal corrosion of the cooling tower, and are easily released into the atmosphere, causing problems of lowering sterilization efficacy. In addition, in a state in which biofilms are formed due to non-specific reactions, first react with polysaccharides secreted before they react with the microorganisms that cause them, and thus, there is a disadvantage in that the effective sterilization effect is lowered.

Non-oxidizing fungicides which overcome such disadvantages are known as 3-isothiazolone, quaternary ammonium compound, formaldehyde releasing compound, pyridine-based compound, glutaraldehyde and the like, and are mainly used alone.

However, these compounds have a weak penetrating effect on organic substances, so that the disinfecting effect is reduced when organic substances or proteins are present, and there is no problem in removing odors, such as ammonia gas or hydrogen sulfide gas, which have already been produced. In addition, when used alone, it emits a halogen compound, such as fluorine, chlorine, which is a corrosive substance, so it is difficult to apply where corrosive metals such as carbon steel, cast iron, stainless steel, and copper are used. Therefore, although quaternary ammonium is used to impart fast-acting effect to the disinfectant, problems such as foaming are caused, which makes it difficult to apply to various industrial fields.

In addition, polyhexamethyleneguanidine phosphate has the disadvantage of being fast-acting and used for more effective and extensive control of microorganisms in various industries, such as in water treatment and disinfectants, and having low foaming, while not having a broad antimicrobial spectrum by itself. Various sterilizing compositions have been provided to overcome this problem by adjusting the mixing ratios of the disinfectants of different strains to improve the disadvantages.

An object of the present invention is to provide a fungicide composition that inhibits the development and growth of various bacteria and fungi as a fast-acting and persistent fungicide.

Another object of the present invention relates to a sterilization method using the fungicide composition.

In order to achieve the above object,

a) polyalkylene guanidine salt represented by the following formula (1); And

b) pyrithione sodium represented by the following formula (2)

It provides a fungicide composition comprising:

In Formula 1,

R ₁ is a C ₃ to C ₁₇ straight or branched alkylene group, wherein optionally at least one hydrogen of the alkylene group is substituted with an N, O or S atom,

X is independently HCl, HBr, HI, HNO ₃ , carbonic acid, sulfuric acid, phosphoric acid, acetic acid, benzoic acid, dehydrogenic acid, propionic acid, gluconic acid, sorbic acid, fumaric acid, maleic acid, lactic acid, malic acid, citric acid, succinic acid, oxalic acid, Selected from the group consisting of tartaric acid, formic acid, glycolic acid and epichlorohydrin acid,

n is an integer from 1 to 100,

m is an integer whose n / m satisfy | fills 0.1-100.

In another aspect, the present invention provides a sterilization method to kill the bacteria, fungi, or algae, or to inhibit growth by injecting the fungicide composition in the area contaminated by bacteria, fungi, or algae using the fungicide composition.

Hereinafter, the present invention will be described in more detail.

The bactericidal composition according to the present invention contains polyalkylene guanidine salt and pyrithione sodium in a certain ratio, and has antimicrobial spectrum against various bacteria, fungi or algae having bactericidal properties and synergism by mixing. It is excellent and applied to various industrial fields.

The polyalkylene guanidine salt is represented by the following general formula (1):

[Formula 1]

In the formula (1)

R ₁ is a C ₃ to C ₁₇ straight or branched alkylene group, wherein optionally at least one hydrogen of the alkylene group is substituted with an N, O or S atom,

X is independently HCl, HBr, HI, HNO ₃ , carbonic acid, sulfuric acid, phosphoric acid, acetic acid, benzoic acid, dehydroacetic acid, propionic acid, gluconic acid, sorbic acid, fumaric acid, maleic acid, lactic acid, malic acid, citric acid, succinic acid, oxalic acid, Selected from the group consisting of tartaric acid, formic acid, glycolic acid and epichlorohydrin acid,

n is an integer from 1 to 100,

m is an integer where n / m satisfies 0.1 to 100.

Preferably, R ₁ is selected from the group consisting of hexamethyldiamine, dodecadiamine, triethyleneglycol diamine, polyoxypropylenediamine, and polyoxyethylenediamine, wherein X is phosphoric acid, hydrochloric acid, gluconic acid and citric acid It may be a salt selected from the group consisting of.

The polyalkylene guanidine salt is a low-toxic fungicide with environmentally friendly properties because it does not contain heavy metals and volatile organic compounds (VOCs) as a bactericidal composition and has no skin irritation and has fast-acting effects. Therefore, it is widely used as an effective disinfectant for more effective and widespread control of microorganisms in various industrial fields such as water treatment and disinfectant, and has the advantage of excellent sterilizing power and no corrosion. However, there is a disadvantage that the antimicrobial spectrum is not wide, which is solved by adding a certain amount of sodium pyrithione.

Pyrithione sodium, which is another component of the fungicide composition according to the present invention, is represented by the following Chemical Formula 2:

[Formula 2]

The pyrithione sodium has an antimicrobial effect against a variety of microorganisms, bacteria and algae, and has the disadvantages of instantaneous sterilization and short-acting effect and corrosion, but this disadvantage is compensated for by using the polyalkyleneguanidine salt as described above.

The mixing ratio of the polyhexamethylene guanidine salt and the pyrithione sodium represented by Formula 1 used in the present invention is preferably a weight ratio of 100: 1 to 300: 1, more preferably 1: 1 to 30: 1. Have a range. If the mixing ratio of the polyalkyleneguanidine salt and the pyrithione sodium is out of the above range, there is a problem that the synergistic effect of the mixing of the two fungicides is reduced or does not appear.

That is, when a mixture of polyalkyleneguanidine salt and pyrithione sodium is used as a disinfectant composition, the pyrithione sodium compensates for the disadvantage that the antimicrobial effect of the polyalkylene guanidine salt decreases against mold, and the pyrithione sodium is fast-acting. Not only the polyalkyleneguanidine salt compensates for the disadvantage that the effect on the bacteria, and slime is reduced, but the sterilization action of the mixed fungicide shows a synergistic effect that is greater than the sum of the sterilization actions of the respective compounds. As a result, the fungicide composition of the present invention can effectively suppress a wide range of microorganisms more effectively by using two kinds of compounds having different sterilization mechanisms, and can obtain an expected effect of lowering the frequency of resistant strains when used alone. .

The fungicide composition according to the present invention may be prepared in various forms such as powders, granules, emulsions, colloids, and the like, and may further include various additives as necessary. For example, the fungicide active ingredient is benzyl alcohol, 2,4-dichlorobenzyl alcohol, 2-phenoxyethanol, 2-phenoxyethanol hemiformal ( 2-phenoxyethanol hemiformal), Phenylethyl alcohol, 5-Bromo-5-nitro-1,3-dioxane, 5-Bromo-5-nitro-1,3-dioxane, Dimethyloldimethylhydantoin Dimethyloldimethylhydantoin, Glyoxal, Glutardialdehyde, Sorbic acid, Benzoic acid, Salicylic acid, p-Hydroxybenzoic acid , Chloroacetamide, N-Methylolchloroacetamide, p-chloro-m-cresol, N-Methylolurea, N , N'-Dimethylolurea, benzyl formal, 4,4-dimethyl-1,3-oxazolidine, 4,4-Dimethyl-1,3-oxazolidine , 1,3,5-hexa-high Rotriazine (1,3,5-Hexahydrotriazine) derivatives, N-alkyl-N, N-dimethylbenzylammonium chloride and di-n-decyldimethylammonium chloride (di-n quaternary ammonium compounds such as -decyldimethylammonium chloride, cetylpyridinium chloride, diguanidine, chlorohexidine, 1,2-dibromo-2,4-dicyanobutane (1,2-Dibromo-2,4-dicyanobutane), 3,5-dichloro-4-hydroxybenzaldehyde, ethylene glycol hemiformal, tetra Tetra (hydroxymethyl) phosphonium salt, Dichlorophene, 2,2-Dibromo-3-nitrilopropionamide, 3-iodine 3-Iodo-2-propynyl-N-butylcarbamate, methyl N-benzimidazol-2-ylcarbamate (Methyl N-benzimidazol-2- ylcarbamate), 2-n-octylisothiazolin-3-one, 4,5-dichloro-2-n-octylisothiazolin-3-one (4,5- Dichloro-2-n-octylisothiazolin-3-one), 4,5-trimethylene-didecyl dimethyl ammonium salt (4,5- Trimethylene-2-methylisothiazolin-3-one), di-N-methyl-2,2 ' Di-N-methyl-2,2'-dithiodibenzamide

C such as 2-thiocyanomethylthiobenzothiazole, 2-hydroxymethyl-2-nitro-1,3-propanediol -Formal, methylene bisthiocyanate, formaldehyde and the reaction of allantoin. Additives include thickeners, antifoams, pH regulators and stabilizers, perfumes, dispersants, coloring agents and discoloration prevention agents such as complexing agents. Stabilizers for preventing decomposition of components are possible.

As described above, the fungicide composition of the present invention is prepared by a method commonly used in the art, further comprising various additive components as described above, in addition to the polyalkyleneguanidine salt and pyrithione sodium. In one embodiment, the composition according to the invention is prepared by adding the pyrithione sodium and polyalkyleneguanidine salts to a solvent in a mixer to uniformly mix.

The fungicide composition is dispersed in a solvent and prepared in various forms in a solid phase such as powders and granules, or in a liquid phase such as an emulsion and a suspension. At this time, the polyalkyleneguanidine salt and the pyrithione sodium in the fungicide composition are each preferably present in a concentration of 1 to 50% by weight, preferably 1 to 30% by weight based on the total fungicide composition.

The solvent may be any one or more of a polar solvent and a nonpolar solvent. As the polar solvent, water; C ₁ to C ₄ lower alcohols including ethanol and isopropanol; Alkylene glycols including ethylene glycol, diethylene glycol, 1,2-propylene glycol, dipropylene glycol and tripropylene glycol; Glycol ethers including butyl glycol and butyl diglycol; Glycol esters including butyl diglycol acetate and 2,2,4-trimethylpentanediol monoisobutyrate; Poly glycols including polyethylene glycol and polypropylene glycol; And N, N-dimethylformamide (N, N-dimethylformamide); at least one selected from the group consisting of is possible. The nonpolar solvent is selected from the group consisting of aromatics such as xylene, toluene and alkylbenzol, nonpolar esters such as paraffins, phthalates and fatty acid esters, epoxidized fatty acids and their derivatives, and silicone oils. One or more selected are possible.

As described above, the fungicide composition according to the present invention is low in toxicity and has no skin irritation, and has a fast-acting effect in a region contaminated by bacteria, fungi, or algae to kill the bacteria, fungi, or algae, Suppress

According to a preferred embodiment of the present invention, the bactericidal composition comprising polyacyleneguanidine salt and pyrithione sodium is prepared by Enterobacter Aerogens ( Enterobacter). aerogens ATCC 13048), Escherichia coli ATCC 11229), Micrococcus luteus ATCC 9341), Pseudomonas aeruginosa ATCC 15442, Klebsiella pneumoniae ATCC 1560, Staphylococcus epidermis ATCC 155), Staphylococcus aureus ATCC 6538), and Bacillus subtilis ATCC 6984 bacteria and Aspergillus As a result of measuring the bactericidal effect on the fungus such as niger ATCC9642), it was possible to obtain a high bactericidal effect even by using a small amount compared to the respective ones. Therefore, the composition may be treated to a region in which various microorganisms, such as fungi, inhabit or grow, thereby ensuring an excellent bactericidal effect.

Specifically, the disinfectant composition according to the present invention may be used to prevent attack by bacteria, filamentous fungi, yeast and algae, paint, rendering, lignosulfonate, chalk standing, etc. Chalk suspensions, adhesives, photochemicals, casein-containing products, starch-containing products, bitumen emulsions, surfactant solutions, fuels, detergents, cosmetic products, cooling water in industrial processes, polymer dispersions, Cooling lubricants, cooling towers in pulp or paper mills, disinfectant disinfectants, paints, latex preservatives, additives in emulsion products such as shampoos, slime control agents, preservatives for leather products and preservatives for metal working oils, antirust agents for textile weaving, and papermaking and It can be used for effective and extensive control of microorganisms in various industries such as.

At this time, the disinfectant composition of the present invention is generally used in the total concentration of 1 to 1,000ppm, preferably 100 to 800ppm based on the entire material to be protected. When used in practice, the fungicide composition is used as a ready mix or by adding the fungicide and optional remaining ingredients of the composition to the material to be protected separately.

Hereinafter, the present invention will be described in more detail with reference to Examples. The following examples are intended to further illustrate the present invention, and the scope of the present invention is not limited by these examples.

[Example]

Example  1 to 5: bactericidal test for bacteria

Enterobacter aerogens of the fungicide composition according to the invention ( Enterobacter aerogens ATCC 13048), Escherichia coli ATCC 11229), Micrococcus luteus ATCC 9341), Pseudomonas aeruginosa ATCC 15442, Klebsiella pneumoniae ATCC 1560, Staphylococcus epidermis ATCC 155), Staphylococcus The sterilizing effect of the 8 types of bacteria aureus ATCC 6538), and Bacillus's Sup-blocks (Bacillus subtilis ATCC 6984) was carried out as follows to see.

(Referred Polyhexamethyleneguanidine phosphate, hereinafter 'PHMG', SKYBIO 1100 ^TM, SK Chemical Co., Ltd.), first (and Sodium 2-pyridinethiol-1-oxide , hereinafter SPT D) to the reactor pyrithione sodium and polyhexamethylene guanidine phosphate by mixing A fungicide composition was prepared.

After diluting the fungicide composition according to the two-fold dilution method using Tryptic Soy Broth of Difco, the culture medium containing the fungicide and the mixed strain was incubated at 30 ° C. for 3 days. Thereafter, the lowest concentration of turbidity that inhibited growth was measured by the method of visual observation, and then the synergistic effect of the fungicide was calculated.

The synergism of the fungicide is Kull, FC et al . According to the method published in the article ( Appl . Microbiol . 9: 538 to 544 (1961)) was measured using the ratio measured by the following equation (1). At this time, when the sum of Qa / QA and Qb / QB is less than 1, it was determined that the synergy appeared, the results are shown in Table 1 below.

In the above equation (1)

Qa is the MIC value of PHMG (ppm) in the PHMG + SPT mixed composition,

Qb is the MIC value (ppm) in the PHMG + SPT mixed composition,

Q _A is the MIC value (ppm) of PHMG alone as the sole ingredient fungicide,

Q _B is the MIC value (ppm) of SPT alone as the sole ingredient fungicide,

MIC (ppm) PHMG: SPT weight ratio SI Qa (PHMG) Qb (SPT) Example 1 78 3 26: 1 0.75 Example 2 78 6 13: 1 1.0 Example 3 39 3 13: 1 0.50 Example 4 39 6 6.5: 1 0.75 Example 5 10 6 1.7: 1 0.56

Q _A : 156 ppm, Q _B : 12 ppm

In Table 1, as shown in Example 2, using only about 1/2 of SPT, even if the amount of PHMG is reduced to 1/2, the same microbial inhibitory effect can be seen.

Therefore, when the two compounds are mixed as in the present invention, the growth of bacteria can be more effectively inhibited than when the two substances are used alone, and the synergistic effect of PHMG and SPT is 13: 1 with respect to the bacterial mixture. It can be seen that it is most preferred when treated in weight ratio.

Example  6 to 9: antiseptic effect test on mold

In order to determine the antiseptic synergistic effect on the fungus of the fungicide composition according to the present invention, by using a fungus Aspergillus niger ( Aspergillus niger ATCC9642) using Potato Dextrose Broth (Difco) To carry out the same as, and to measure the rising index based on Equation 1 shown in Table 2.

MIC (ppm) PHMG: SPT weight ratio SI Qa Qb Example 6 156 39 4: 1 0.75 Example 7 78 39 2: 1 0.62 Example 8 78 20 4: 1 0.38 Example 9 39 39 1: 1 0.56

Q _A : 626 ppm, Q _B : 78 ppm

As shown in Table 2, it can be seen that in Example 8, the same microbial inhibitory effect can be obtained by using only 1/4 of the SPT for the PHMG content and reducing the amount of polyhexamethyleneguanidine phosphate to 1/8. Can be.

Therefore, mixing the two compounds of PHMG and SPT according to the present invention can effectively inhibit the growth of mold than when using each alone, and the synergistic effect of PHMG and SPT is to mix each compound against the mold and bacteria mixture It can be seen that the most preferable when used in a mixed weight range of 1: 1 to 4: 1.

Example  10 and Comparative example  1 to 2

A sterilization composition containing PHMG and SPT in a weight ratio of 1: 1 was used for cooling tower process water and four individual strains ( Staphylococcus). aureus ATCC 6538, Escherichia col i ATCC11229, Micrococcus leteus ATCC9341, Aspergillus niger ATCC 9642) to determine the bactericidal effect of each.

The MIC test was carried out by diluting the fungicide according to a 2-fold serial dilution method using a 24 multi well plate, inoculating the microorganism at a concentration of 10 ⁴ CFU / mL, and incubating for 48 hours at 30 ° C., followed by turbidity. MIC value was measured by the method of visual inspection based on the reference | standard. At this time, the turbidity was observed using Dipco's Tryptic Soy Broth (Difco Co.), and the obtained results are shown in Table 3 below.

At this time, the disinfectant composition of Example 10 is a mixture of PHMG and SPT in a weight ratio of 1: 1, SPT alone in Comparative Example 1, Comparative Example 2 is a commercial water treatment product Vantocil IB and SPT having a structure similar to PHMG 1: It was used by mixing in a weight ratio of 1 dissolved in water 30% by weight.

Microorganism MIC (ppm) Comparative Example 1 Comparative Example 2 Example 10 E. coli 9.7 19.5 9.7 M. leteus 9.7 4.9 4.9 Stafford Philo Cocos aureus (S. aureus) 9.7 9.7 9.7 Aspergillus Niger (A. niger) 78 156 156  Cooling tower water 156 156 78

As can be seen in Table 3, it can be seen that it is more effective to control the microorganisms using the same mixture as in Example 10 than using SPT alone as in Comparative Example 1 for various microorganisms.

In addition, Vantocil IB and SPT components showed a better effect on the actual cooling tower water compared to the sterilization composition mixed in a 1: 1 ratio.

Example  11 to 12, and Comparative example  3 to 5

The fungicide mixture used in Example 10 and the solution used in Comparative Example 2 were carried out as follows to determine the killing time, killing time, and sustainability of the microorganisms.

500 ppm (Example 11) and 1,000 ppm (Example 12) of the mixture of Example 10 and 500 ppm of the fungicide mixture of Comparative Example 2 were respectively added to the cooling water of the polymerization cooling tower having a concentration of microorganism of about 10 ⁴ CFU / mL. 3), 1,000 ppm (Comparative Example 4) was added to observe the activity of the microorganisms up to 80 hours. In this case, the solution without the sterilizer was added as a blank, and the obtained result is shown in FIG. 1.

1 is a graph showing the activity of microorganisms over time.

Referring to FIG. 1, the fungicide mixture of Comparative Example 3 exhibited microbial activity after 42 hours, and the sustainability of the bactericidal effect was maintained for 42 hours, and Comparative Example 4 maintained the bactericidal effect for 50 hours. In comparison, the disinfectant composition of Examples 11 and 12 according to the present invention maintained microbial activity at a level of about 1/3 compared to the blank after 20 hours and continued disinfection until 62 hours.

Therefore, the disinfectant composition according to the present invention has excellent sterilizing effect even when the polyalkyleneguanidine salt and SPT are used in a mixed amount compared to the respective MICs. Furthermore, the disinfectant composition is low in toxicity and has no skin irritation, and can be suitably applied to a water treatment apparatus in which various bacteria and fungi proliferate, as well as disinfecting and cosmetic products which have fast-acting contact with the skin.

Claims (8)

a) polyalkylene guanidine salt represented by the following formula (1); And b) pyrithione sodium represented by the following formula (2) Fungicide composition comprising as an active ingredient: [Formula 1]

In Formula 1, R ₁ is a C ₃ to C ₁₇ linear or branched alkylene group, at least one hydrogen of the alkylene group is each independently substituted with an N, O or S atom, X is independently HCl, HBr, HI, HNO ₃ , carbonic acid, sulfuric acid, phosphoric acid, acetic acid, benzoic acid, dehydrogenic acid, propionic acid, gluconic acid, sorbic acid, fumaric acid, maleic acid, lactic acid, malic acid, citric acid, succinic acid, oxalic acid, Selected from the group consisting of tartaric acid, formic acid, glycolic acid and epichlorohydrin acid, n is an integer from 1 to 100, m is an integer whose n / m is 0.1-100. [Formula 2]

The method of claim 1, R ₁ is selected from the group consisting of hexamethyldiamine, dodecadiamine, triethyleneglycoldiamine, polyoxypropylenediamine, and polyoxyethylenediamine, and X is selected from the group consisting of phosphoric acid, hydrochloric acid, gluconic acid and citric acid Fungicide composition. The method of claim 1, The fungicide composition is a fungicide composition is a polyalkyleneguanidine salt: pyrithione sodium is mixed in a weight ratio of 1: 100 to 100: 1. The method of claim 1, The fungicide composition comprises a polyalkyleneguanidine salt and pyrithione sodium salt in an amount of 1 to 10,000 ppm based on the total composition. The method of claim 1, The fungicide composition is Enterobacter Aerogens ( Enterobacter aerogens ), Escherichia coli ), Micrococcus , Pseudomonas Pseudomonas aeruginosa ), Klebsiella pneumoniae ), Staphylococcus epidermis ), Staphylococcus aureus ), Bacillus subtilis subtilis ) and Aspergillus niger . A fungicide composition having a bactericidal effect against at least one microorganism selected from the group consisting of. The method of claim 1, The composition further comprises one or more additives selected from the group consisting of thickeners, antifoams, pH regulators, stabilizers, perfumes, dispersants, and complexing agents. Fungicide composition comprising. A method for sterilizing microorganisms, wherein the fungicide composition according to any one of claims 1 to 6 is used in the form of an emulsion or suspension dispersed in a powder, granule, solvent. The method of claim 7, wherein A sterilization method using the sterilant composition in a concentration of 1 to 100,000 ppm.

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