KR101240506B1 - Composition - Google Patents

Abstract

PURPOSE: A liquid composition for promoting culture and fermentation of useful microorganisms is provided to quickly reach a target level. CONSTITUTION: A liquid composition for promoting culture and fermentation of useful microorganisms contains 2000 parts by weight of bay salts, 100 parts by weight of citric acid, 30 parts by weight of xylo-fructose, 1000 parts by weight of blown sugar, 4000 parts by weight of glucose, and 5 parts by weight of xylitol. An apparatus for culturing the useful microorganism comprises: a culture tank(130), a direct water supply pipe(230), a raw liquid supply pipe(200), a stirring unit, a heating unit, a culture rate measuring unit, and a control unit(310).

Description

Useful microorganism cultivation and fermentation broth composition

The present invention relates to a culture and fermentation promoting liquid composition of useful microorganisms, and more particularly, 2000 parts by weight of natural salt in culture and fermentation promoting liquid of useful microorganisms cultured in an integrated culture apparatus for propagation and supply of useful microorganisms, citric acid 100 It relates to a useful microorganism cultivation and fermentation broth composition consisting of a weight part, 30 parts by weight of gyrosaccharide, 1000 parts by weight of brown sugar, 4000 parts by weight of glucose and 5 parts by weight of xylitol.

The quality of human beings is depressed because they are living on the concrete ground due to the destruction of the environment due to urbanization and the centralization of buildings.

In order to improve the quality of life and the quality of the city, the campaign to revitalize the stream is showing effectiveness, but there is no fresh water flowing in and the quality of the streams and rivers that circulate or flow by using the Han River fall. There is a problem that the odor is generated by the growth of microorganisms.

In particular, the most important form of nitrogen flowing into the sewage and wastewater treatment plant is ammonia nitrogen, which is a nutrient for algae, and 70% of the total oxygen required in the nitrification process is consumed in this process. Ammonia nitrogen consumes a large amount of chlorine in chlorine disinfection, and when gas is volatilized, it causes odors and causes odor complaints in sewage and wastewater treatment plants. The conventional facility structure causes a number of serious problems, such as occupying a large installation area and increasing the size of the apparatus, as well as increasing the construction cost and the maintenance cost. In addition, the situation is a factor that greatly reduces the utilization efficiency of the equipment, it is more compact, installed in a high efficiency and low cost, the nitrogen component in the sewage and wastewater is removed, which can reduce the odor while maintaining sound water quality There is an urgent need for a device. There is a problem that management is difficult because there is no integrated system of the culture tank compactly formed such a facility.

The present invention in order to improve the above problems in the culture and fermentation promoting solution of useful microorganisms cultured in an integrated culture device for the growth and supply of useful microorganisms 2000 parts by weight of natural salt, 100 parts by weight of citric acid, 30 parts by weight of gyro fructose, brown sugar It is an object to provide a culture and fermentation promoting liquid composition of useful microorganisms consisting of 1000 parts by weight, 4000 parts by weight of glucose and 5 parts by weight of xylitol.

In the present invention, 2000 parts by weight of natural salt, 100 parts by weight of citric acid, 30 parts by weight of gyro fructose, 1000 parts by weight of brown sugar, and 4000 of glucose in the culture and fermentation promoting solution of useful microorganisms cultured in an integrated culture device for the growth and supply of useful microorganisms. It relates to a useful microorganism culture and fermentation broth composition consisting of parts by weight and 5 parts by weight of xylitol.

The culturing and fermentation promoting liquid composition of the useful microorganism of the present invention takes 7 to 10 days to achieve the desired value when culturing and fermentation of the useful microorganism is not used, but the culturing and fermentation promoting liquid of the useful microorganism If used, it takes 3 to 5 days for culture and fermentation to reach the desired values.

1 is a perspective view showing an integrated culture apparatus for propagation and supply of useful microorganisms.
Figure 2 is a cross-sectional view showing an integrated culture apparatus for propagation and supply of useful microorganisms.
Figure 3 is a state configuration of the opening and closing door of the integrated culture device for propagation and supply of useful microorganisms.
Figure 4 is a cross-sectional view of a state configured so that the stirring of the raw material and the direct water well.

The present invention is a culture tank 130 consisting of an outer cylinder 120 and an outer cylinder 120 on the outer surface of the insulator configured to increase the thermal insulation power to maintain a proper temperature on the inner surface of the inner cylinder 100 and the cylindrical structure of the cylindrical structure. ; Culture fluid stirring unit 190 comprising a motor rod 180 including a shaft (150) and a fixed bearing plate (160) with a bearing built therein to secure the shaft rods are arranged at regular intervals; It consists of a feed solution supply pipe 200 configured to supply the culture solution and the electronic opening and closing valve 210 and a direct water supply pipe 230 configured to configure the electronic opening and closing valve to supply the raw water, and the mixed solution supply pipe and the direct supply supply pipe are connected to each other to form a mixing pipe 370. A), wherein the mixing pipe 370 is formed of a plurality of partitions in the discharge passage so that the culture solution is easily discharged when the raw water is discharged and configured to form a stirring barrel 410 to be stirred with each other while passing through the partition; A discharge part including a vent port 250 for discharging the internal gas generated during the cultivation to the outside and a discharge pipe 260 for discharging the culture medium to the outside; A heating unit configured of a heater 270 configured to maintain a temperature in the culture tank at 25 to 30 degrees and a temperature detection sensor 280 for sensing a temperature; A culture rate meter 330 for detecting the pH of the culture state by detecting the progress of the culture; Control the feed rate of the stock solution and the raw water, control the speed and rotation speed of the culture stirring plate 190 to mix the culture solution, control the temperature of the heating unit to maintain the optimum temperature of the culture, and maintain the culture state in the culture tank. In the real-time culturing and fermentation promoting liquid composition of useful microorganisms cultivated in the integrated culture apparatus for propagation and supply of useful microorganisms consisting of a control unit 310 to check in real time to inform the discharge time to the lamp or alarm, and integrated control to discharge The present invention relates to a useful microorganism culturing and fermentation broth composition, comprising 2000 parts by weight of natural salt, 100 parts by weight of citric acid, 30 parts by weight of gyrosaccharide, 1000 parts by weight of brown sugar, 4000 parts by weight of glucose, and 5 parts by weight of xylitol.

The integrated culture apparatus for propagation and supply of useful microorganisms of the present invention shown in FIGS. 1 to 3 is configured to integrate the apparatus capable of producing a large amount of EM to minimize the manufacturing cost and to conveniently configure the equipment operation. The culture apparatus of the present invention comprises a culture tank, a direct water supply pipe, a stock solution supply pipe, a stirring part, a heating part, a culture rate axis part, a control part.

As shown in Figure 2, the integrated culture apparatus for propagation and supply of useful microorganisms of the present invention, the culture apparatus is configured to increase the thermal insulation power to maintain a suitable temperature on the inner surface of the inner cylinder 100 and the outer surface of the inner cylinder of the cylindrical structure Culture tank 130 consisting of a heat insulating material 110 and an outer cylinder 120 on the outer surface of the heat insulating material; The motor unit 180 including the fixed plate 160 and the reducer 170 are built in the upper portion of the culture tank and the blade 140 is fixed to the shaft rod 150 is arranged at regular intervals and the bearing is fixed to the shaft rod. Culture medium stirring unit 190; A supply unit configured at an upper portion of the culture tank, the feed solution supply pipe 200 supplying a culture solution and a direct water supply pipe 230 supplying water; The top of the culture tank

It is configured in the lower portion, the exhaust port consisting of a vent port 250 for discharging the internal gas generated during the cultivation to the outside, and a discharge pipe 260 for discharging the culture medium to the outside; A heater configured to include a heater 270 configured to maintain a temperature in the culture tank at 25 to 30 degrees, a temperature sensor 280 for sensing a temperature, and a temperature display 290; A culture rate meter 330 for detecting the pH of the culture state by detecting the progress of the culture; Control the feed rate of the stock solution and the feed water, control the speed and rotation speed of the culture medium stirring part to mix the culture solution, control the temperature of the heating part to maintain the optimum temperature of the culture, and check the culture state in the culture tank in real time The controller 310 is configured to notify the discharge time to the lamp or alarm, and to control the discharge as possible.

The culture tank 130 may be composed of a stainless steel material or the inner cylinder 100 and the outer cylinder 110 may be composed of a synthetic resin material. In addition, the culture tank is formed in a cylindrical structure and is formed in a double structure to maintain the temperature required for cultivation is filled with a heat insulating material (110) in between. The culture fluid stirring unit 190 is configured on the top of the tank and the blades 140 constitute the shaft bar 150 is arranged at regular intervals, the tank is fixed to the fixed plate 160 with a bearing built in to secure the shaft rod Fasten firmly to the top of the unit. The motor unit 180 including the reducer 170 is configured to slowly rotate the rotation speed of the shaft, and is configured to mix the culture solution and the raw water evenly.

In addition, the rotational speed of the shaft and the rotational speed according to the incubation process to be different by the program.

The supply part is composed of a feed solution supply pipe 200 for supplying the culture solution and a direct water supply pipe 230 for supplying the raw water, and configures the electronic opening and closing valves 210 and 230 to automatically open and close the feed solution supply pipe and the direct supply supply pipe. In addition, it is preferable to use water in a state in which chlorine is removed from raw water.

One example of the major EM fine microorganisms to be cultured is photosynthetic bacteria: carbon dioxide and hydrogen compounds are used to survive under high pressure and temperature conditions, while synthesizing organic compounds and generating oxygen for photosynthesis. Lactobacillus: A microorganism with a strong ability to preserve perishables and preserve it, and act as the first fermentation of organic matter for EM.

do. Actinomycetes: The amino acids produced by photosynthetic bacteria produce antibacterial substances (such as streptomycin). Yeast: It performs various fermentations, grows well at low acidity, and produces useful substances for crops such as amino acids, sugars, and other soil organic matter emitted by photosynthetic bacteria.

In order to produce such an EM culture in large quantities, an optimum culture apparatus was developed. The discharge part is configured in the upper and lower portions of the culture tank 130, the vent port 250 for discharging the internal gas generated during the cultivation to the outside, and the discharge pipe 260 for discharging the culture medium to the outside, The automatic opening and closing valve 300 is configured to automatically control the discharge by controlling the discharge time.

The vent port 250 may be configured as a blower fan for naturally discharging gas or forced discharge. The discharge pipe 260 is formed at the bottom of the culture tank, and since the entire amount of the culture solution is not discharged at all during discharge of the culture medium, and a certain amount remains, the discharge pipe 260 has an appropriate amount necessary for culturing the microorganisms without introducing the microorganisms daily. It is possible to continuously supply and cultivate the required microorganisms by supplying the nutrient source and automatically supplying it according to the culture cycle schedule of the microorganisms set in advance through the control, and cultivation is completed through the discharge pipe 260.

In the present invention, when the microorganisms are cultured by adding a nutrient source to the culture tank, the measurement result shows that the culture efficiency of the microorganisms reaches its peak at about 75 to 90 hours. Therefore, the culture medium supply cycle of microorganisms is set accordingly.

The heating unit is composed of a heater 270 configured to maintain the temperature in the culture tank 25 to 30 degrees and a temperature sensor 280 for sensing the temperature. The heater 270 is configured to maintain a constant temperature in the culture tank. The heater can be configured in one or the top and bottom respectively. The heater is detected by the temperature sensor 280 to maintain the proper temperature for the culture

Configure on or off.

In addition, since the external element of the growth of useful microorganisms (EM) requires a device for circulating or moving the culture at a constant temperature of 25 to 30 degrees, the inner cylinder 100 is made of stainless steel or synthetic resin, and the culture is evenly distributed in a cylindrical structure. Culture temperature stirring unit 190 and the culture rate measuring instrument 330 to know the culture rate to be mixed, and the temperature display unit 290 is configured to detect and display the operation of the heater by the temperature sensor 280, and automatically temperature It is configured to control, and cultivation after release of the culture medium automatically supplies raw water and EM stock and nutrients (molasses, etc.) suitable for the culture tonnage so that it can be cultivated smoothly to configure the present invention in a package (integrated) form.

The culture rate meter 330 was configured to detect and display the initial pH concentration of the mixed solution in the stirred state and the pH concentration cultivated in real time, and configured to receive the detection value by the controller 310. It can also be installed in other locations in order to accurately determine the culture state in the culture tank.

The control unit 310 is controlled to supply at a set ratio of the stock solution and the water supply, and each nutrient source is supplied indiscriminately into the culture tank from the stock solution tank, and the amount of nutrient source necessary for culturing microorganisms is controlled by adjusting the nutrient source input. Becomes The input of each of these nutrient sources is necessary for the batch culture of microorganisms from the nutrient tank.

When only the quantity is supplied and this one-time nutrient supply operation is completed, it is automatically adjusted and controlled to be introduced into the culture tank through the supply pipe connected with the nutrient tank again.

The speed and rotation speed of the culture medium stirring unit to control the culture solution was configured to prevent unnecessary stirring. In addition, by controlling the temperature of the heater of the heating unit to maintain the optimum temperature of the culture is configured to check the temperature sensor in real time to control the heater to maintain a constant temperature.

In addition, it checks the culture state in the culture tank in real time to inform the discharge time to the lamp or alarm, and to control the discharge as integrated.

As shown in FIG. 3, the door 340 is configured in the integrated culture apparatus for propagation and supply of useful microorganisms of the present invention, and the door is configured to be cleaned and repaired. Supply unit of the integrated culture apparatus for propagation and supply of useful microorganisms of another embodiment of the present invention shown in Figure 4 when the microorganism and nutrients, or when mixing the raw water and raw materials stirring tank 410 so as to easily mix first Configure

The supply part constituted a single feed pipe by connecting the direct water supply pipe and the raw material supply pipe by configuring the raw water supply pipe 230 having the electromagnetic open / close valve 240 and the raw liquid supply pipe 200 having the electronic open / close valve 210. The mixing pipe 370 is configured so that the raw liquid (culture liquid) is easily discharged when the raw water is discharged and the through hole so that the discharged raw liquid is well blended with the raw water

Forming a plurality of partitions formed in multiple stages to form a stirring barrel 410 to be stirred while passing through the partition. It is configured to be smoothly mixed at the time of discharge as described above to be discharged to the supply pipe through the flow pipe 370 between the partition. The end of the supply pipe 380 is configured to automatically supply the electronic opening and closing valve 400

And blocking control is possible. Useful microorganisms (EM) cultured as described above can remove the odor, air purification and water quality improvement.

Example

2000 g of natural salt, 100 g of citric acid, 30 g of gyrosaccharide, 1000 g of brown sugar, 4000 g of glucose and 5 g of xylitol were incubated in an integrated culture apparatus for propagation and supply of useful microorganisms. .

If the culture and fermentation promoting liquid of the useful microorganism is not used, it takes 7 to 10 days for the culture and fermentation to reach the desired value, but if the culture and fermentation promoting liquid of the useful microorganism is used, the culture and fermentation reach the desired value. It takes 3 to 5 days to complete.

100: inner cylinder 110: insulation
120: outer cylinder 130: culture tank
140: wing 150: shaft
160: fixed plate 170: reducer
180: motor portion 190: culture solution stirring portion
200: feed solution supply pipe 210,240,300: automatic opening and closing valve
220: pedestal 230: direct water supply pipe
250: vent port 260: discharge pipe
270: heater 280: temperature sensor
290: temperature display unit 310: control unit
320: water level sensor 330: culture rate meter

Claims (1)

A culture tank consisting of an outer cylinder on the outer surface of the heat insulating material and the heat insulating material configured to increase the thermal insulation in order to maintain a suitable temperature on the inner surface of the inner cylinder and the cylindrical structure; A culture medium stirring part comprising a motor rod including a shaft having a blade arranged at regular intervals and a fixed plate having a bearing therein and a reducer to fix the shaft; A supply unit configured of a feed solution supply pipe configured to supply the culture solution and an open / close water supply pipe configured to supply the raw water; A discharge part consisting of a vent port for discharging the internal gas generated during the cultivation to the outside and a discharge pipe for discharging the culture medium to the outside; A heating unit configured of a heater configured to maintain the temperature in the culture tank and a temperature sensor for sensing the temperature; A culture rate measuring device that detects the progress of the culture and checks the pH of the culture state; It controls to supply the ratio of the stock solution and the raw water, controls the speed and rotation speed of the culture solution stirring part to mix the culture solution, controls the temperature of the heating part to maintain the optimum temperature of the culture, and checks the culture state in the culture tank in real time. In the culture and fermentation promoting liquid of the useful microorganisms cultivated in the integrated culture apparatus for propagation and supply of useful microorganisms consisting of a control unit that notifies the discharge time to the lamp or alarm, and integrally controls the discharge,
The promoter is a natural salt of 2000 parts by weight, citric acid 100 parts by weight, gyro fructose 30 parts by weight, brown sugar 1000 parts by weight, glucose by weight and xylitol 5 parts by weight of the useful microorganism culture and fermentation promoting liquid composition comprising.

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