KR101233788B1 - Natral Antifungal Agent Containing Phytoncide extracted from Cone of Korean Pine and Material for Preventing Disease of Fruits Cointaining The Same - Google Patents

Abstract

Natural antimicrobial agent prepared by mixing phytoncide extracted from pine conifer and thymol or spearmint, and material for disaster prevention of fruits using the same.

Description

Natural Antifungal Agent Containing Phytoncide extracted from Cone of Korean Pine and Material for Preventing Disease of Fruits Cointaining The Same}

The present invention relates to a natural antimicrobial agent containing a phytoncide extracted from pine nut conifer and a freshness-maintaining material of fruit comprising the same.

Pest control on crops mainly uses organic synthetic pesticides, which is a burden on the environment, residues on agricultural products, and soil pollution. Therefore, research is being conducted to apply safe biological control methods to natural ecosystems.

Among biological pesticides, the development of pest control agents using natural substances with high safety for the environment and human body is being actively conducted, but they do not have sufficient antibacterial effects due to insufficient antimicrobial activity or due to the strong volatility or degradability of natural substances themselves, There are problems such as disappearing.

Pine tree phytoncide is already used for toothpaste, gum and cosmetics because it has excellent fragrance and antibacterial activity.If it maximizes the antimicrobial activity of this substance, it can be used as an optimal natural antibacterial agent for controlling crop diseases occurring in fresh fruits and vegetables. .

The problem to be solved by the present invention is to improve the antimicrobial activity of the pine phytoncide to be used as a natural antibacterial agent.

Another problem to be solved by the present invention is to improve the sustained release of the natural antibacterial agent containing pine phytoncide to provide a freshness maintaining material of the fruit.

The problems of the present invention are not limited to the above-mentioned problems, and other problems not mentioned can be clearly understood by those skilled in the art from the following description.

Natural antimicrobial agent according to an embodiment of the present invention includes phytoncide and thymol.

Natural antibacterial agent according to another embodiment of the present invention includes phytoncide and spearmint.

Here, the phytoncide may be extracted from pine nut conifer, the thymol is included in the active ingredient of the natural antimicrobial agent 4 ~ 30% by weight, the phytoncide may include 70 ~ 96% by weight.

The active ingredient of the natural antimicrobial agent may include 4 to 6 wt% of thymol, and the phytoncide may include 94 to 96 wt%.

In the active ingredient of the natural antimicrobial agent, the spearmint may be included in an amount of 4 to 30% by weight, and the phytoncide may be included in an amount of 70 to 96% by weight.

The active ingredient of the natural antimicrobial agent may include 4 to 6% by weight of the spearmint, and the phytoncide may include 94 to 96% by weight.

The natural antimicrobial agent may have a formulation selected from a film, an emulsion, an emulsifier, a hydrating agent, a water soluble, an aerosol, a spray, a suspension, a granule, a powder, a particulate, a capsule, a bead, a fumigant, a smoker, and a smokescreen.

According to another embodiment of the present invention, the disease-prevention maintenance material of fruit comprises a natural antimicrobial agent including phytoncide and thymol or phytoncide and spearmint, and a biodegradable starch mixed with the natural antimicrobial agent to increase the sustained release of the natural antimicrobial agent. Include.

The mixture of the natural antimicrobial agent and the biodegradable starch may be microencapsulated.

It can be used as a natural anti-regulator by maximizing the antimicrobial power by mixing an appropriate amount of thymol or spearmint in phytoncide extracted from the pine conifer. It can be used as a material for maintaining the freshness of fruit by ensuring sustained release by mixing it with a carrier such as biodegradable starch.

1 is a schematic diagram of a phytoncide extracting apparatus according to an embodiment of the present invention.

BRIEF DESCRIPTION OF THE DRAWINGS The above and other features and advantages of the present invention will be more apparent from the following detailed description taken in conjunction with the accompanying drawings, in which: FIG. The present invention may be embodied in many different forms and is not limited to the embodiments described herein. In order to clearly illustrate the present invention, parts not related to the description are omitted, and the same reference numerals are used for the same or similar components throughout the specification. In the case of publicly known technologies, a detailed description thereof will be omitted.

<Examples>

1) Preparation of Pine Coniferous and Phytoncide

As shown in FIG. 1, pine conifers are introduced into a distillation tank 1 of an essential oil extraction device including a distillation tank 1, a cooling device 2, an oil / water separator 3, and a steam generator 4. The boiler of the steam generator 4 is operated to generate steam and to pass the condensation contained in the distillation tank 1. At this time, the temperature in the distillation tank is preferably 80 to 120 degrees Celsius, the pressure is set to atmospheric pressure, it is also possible to reduce the pressure or pressurized. While passing through the distillation tank, the steam mixed with the essential oil components is condensed while passing through the cooling device (2). The condensate condensed in the cooling device (2) is collected in the oil water separator (3), and the refined oil having a specific gravity lower than water floats on it, so that a light yellow phytoncide oil can be obtained through the valve (a) at the top of the oil water separator (3), Hydrosol is obtained through valve (b) at the bottom of oil / water separator (3).

As such, when the phytoncide is extracted using the pine nut conifer remaining after harvesting the pine tree, the phytoncide of the constituents is less likely to be damaged than the extract of the phytoncide essential oil from the pine stalks, branches or leaves. Table 1 below compares the main components of phyton cheese extracted from pine cones and phytoncide extracted from leaves and resin.

[Table 1]

As can be seen in Table 1 above, the conifers of the pine tree contain different components from the leaves and resin, and the phytoncide extracted here also has a difference in components. This component difference can also affect the efficacy as a natural antimicrobial agent, it is easier to extract the phytoncide from the conifer of pine.

2) Preparation of thymol

Timol is a type of phenol, also known as 2-isopropyl-5-methylphenol. The chemical formula is C ₁₀ H ₁₄ O, colorless crystals with a smell similar to musk colostrum, almost insoluble in water, but soluble in organic solvents such as ethanol and ether. The thymol is not only used as a synthetic raw material of menthol, as an antiseptic, as a fungicide, but also as a toothpaste, soap, etc., and used for analysis of titanium ammonia and the like as an analytical reagent, and also used as an insect repellent and the like.

Such thymol can be extracted by using a thymol-containing civet colostrum or nectar and plant essential oils, such as supercritical extraction, steam distillation, organic solvent extraction. Timols are Dill, Lavender, Lemon balm, Rosemary, Mint, Savory, Savory, Sage, Scentedgeranium, Self Heel hill, Sweet Basil, Chervil, Thyme, Tansy, Cinnamon, Clove, Eucalyptus, Lemon, Peppermint And a plant essential oil component such as Tea tree can be extracted from the plant using the above supercritical extraction method, steam distillation method, organic solvent extraction method and the like. In this example, thymol was purchased from Japan's Pure Chemical Co., Ltd., thymol (purity 98.0% melting point 49 ~ 51 ° C, boiling point 232 ° C, specific gravity 0.965) was used.

3) preparation of spearmint

Spearmint is a perennial herb of Lamiaceae, which has a sweet and strong refreshing scent and is known to have antiseptic disinfection, healthy tonic, soothing and analgesic effects. Spearmint oil is often contained at the tip of leaves or flowers, and can be extracted by steam distillation, supercritical extraction, and the like. Spearmint oil used in this example was purchased from Seoul Inc.

Spearmint contains unsaturated carbon, limonene, and the like and has soothing, analgesic, and skin tissue relaxation effects. Spearmint oil has been used for a long time as a pivoprotectant, an anti-emetic agent, a neuro stabilizer, and a digestive agent, and has been used as a fragrance ingredient in soaps, toothpastes, gums, candies, and beverages, and thus is known to be safe for the environment.

Analysis of spearmint oil by Gas Chromatography (GC) and Mass Spectroscopy (GC / MS) showed that the main components were 16.1% of limonene, 13.8% of gamma-terpinene, and p-cymene. ) 5.8%, 3-octanol 6.9%, carvone 40.9%.

4) Phytoncide and thymol mixtures

A mixture of phytoncide and thymol extracted from pine cones was prepared. In order to measure the antimicrobial activity of the substance, the mixture was added to various strains and the growth inhibition of each strain was measured after 7 days. To compare the antimicrobial activity of various phytoncide and thymol mixtures, thymol stock solution, thymol and pure water, thymol dilution diluted 1/10, phytoncide stock solution (95%) and thymol stock solution (5%), The test was performed by preparing a mixed dilution solution containing a phytoncide stock solution (95%) and a thymol dilution solution (5%) diluted in 1/10, a phytoncide stock solution, a phytoncide solution and pure water, and diluting a 1/10 diluted phytoncide solution. The results are shown in Table 2 below.

[Table 2]

Here, B.cin is Botrytis cinerea, A.alt is Altanaria alternata, C.acu is an anthrax (Colletotricum acutatum), and F.oxy is a bulbous rot fungus (Fusarium) oxysporum), SS means Sclerotinia sclerotiorum, and R.sol means Rhizotonia solani.

As can be seen in Table 2 above, the mixture of the phytoncide stock solution and the thymol stock solution inhibits the growth of A.alt, C.acu, F.oxy and the like to about 70% or more, and B.cin and It also inhibited the growth of R. sol to some extent, showing the best antibacterial activity.

The antimicrobial activity of the phytoncide and thymol mixture was maintained to some extent in the range of 70 to 96% of the phytoncide stock solution and 4 to 30% of the thymol stock solution, and 94 to 96% of the phytoncide stock solution and 4 to 6% thymol solution. It showed the best antibacterial activity in the mixed range.

5) Phytoncide and Spearmint Mixture

A mixture of phytoncide and spearmint extracted from pine cones was prepared. In order to measure the antimicrobial activity of the substance, the mixture was added to various strains and the growth inhibition of each strain was measured after 7 days. In order to compare the antimicrobial activity of various phytoncide and spearmint mixtures, the spearmint stock solution, the spearmint dilution mixture (1%) and the spearmint stock solution (5%) and thymol stock solution (95%) Mixed stock, spearmint stock (5%) and thymol mixed with pure water diluted 1/10 dilute spearmint dilution (95%), phytoncide stock (95%) and spearmint stock (5%) The test mixture was prepared by mixing a mixed stock solution, a phytoncide dilution (95%) diluted with 1/10, and a mixed dilution solution containing spiamine stock solution (5%), respectively, and the results are shown in Table 3 below.

[Table 3]

Here, B.cin is Botrytis cinerea, A.alt is Altanaria alternata, C.acu is an anthrax (Colletotricum acutatum), and F.oxy is a bulbous rot fungus (Fusarium) oxysporum), SS stands for Sclerotinia sclerotiorum, R.sol stands for Rhizotonia solani, KC08 stands for penicillin expansum, JW81 stands for Penicillin expansum, and Pi stands for penicillin italicum. .

As can be seen in Table 3 above, the mixture of the phytoncide stock solution and the spearmint stock solution inhibits the growth of B.cin, F.oxy, A.alt, C.acu and the like by more than 40%, and KC08, JW81 The growth of, Pi and R.sol was also inhibited to some extent, showing the best antibacterial activity.

The antimicrobial activity of the phytoncide and spearmint mixture was found to maintain a certain effect in the range of 70 to 96% of the phytoncide stock solution and 4 to 30% of the phytide stock solution, and 94 to 96% of the phytoncide stock solution and 4 to It showed the best antibacterial activity in the range of 6%.

In order to secure the sustained release of the natural antimicrobial agent of the present invention and to provide a usable form, the following use examples are given.

<Use example>

1) weapon carrier

Carriers that can be blended to produce sustained-release pesticide granules are not particularly limited, but for example, general soil, bentonite, calcium carbonate, diatomaceous earth, zeolite, vermiculite, acid clay, pumice, white carbon (hydrophilic silica, hydrophobic silica), Titanium oxide and the like. Although the binder which can be mix | blended with the sustained-release pesticide granulation of this invention is not specifically limited, For example, polyvinyl alcohol, dextrin, carboxymethylcellulose, polyvinylpyrrolidone, sodium alginate, calcium lignin sulfonate, etc. are mentioned. Examples of stabilizers that can be incorporated into the sustained-release pesticide granules of the present invention include phenolic antioxidants, amine antioxidants, phosphorus antioxidants, sulfur antioxidants, epoxidized soybean oil, epoxidized linseed oil, ethylene glycol, and the like. Can be mentioned. Examples of the lubricant that can be blended into the sustained-release pesticide granules of the present invention include talc, calcium stearate, liquid paraffin, polyethylene glycol, and the like.

2) Organic Capsule

Biodegradable starch may be used as a coating material for microencapsulating phytoncide. 100 g of biodegradable modified starch 30% aqueous solution was added 10 g of phytoncide 교반 and stirred for 10 minutes at 7000 rpm with a homogenizer to prepare a microcapsule slurry solution. This slurry liquid is sprayed using the spray dryer on the conditions of the nozzle part temperature of 80 degreeC, and the nozzle spray rate of 8-9 mL / min, and the powdery microencapsulated phytoncide is obtained.

Although such micro-encapsulated phytoncide may be used as it is, in order to be mixed with a water repellent or the like and have a heat resistance of about 160 degrees Celsius, it may be used as a coumarin resin or curdlan as a film.

The form of use of the natural antimicrobial agent of the present invention is not limited to capsules, films, emulsions, emulsifiers, hydrating agents, water soluble agents, aerosols, pump sprays, suspensions, granules, powders, granules, capsules, beads, fumigants, It may also be in the form of smoke, smoke or disinfectant.

These natural antimicrobial agents have many functions such as multiplicity, antimicrobial, deodorant, freshness maintenance, growth control function according to concentration, and can be useful for manufacturing agricultural, packaging and storage containers. In order to apply to the fruit bag to be coated with a water repellent or after the water repellent coating can be applied by a method such as spray coating, it can be applied to the storage by spraying or the like.

3) film

Silica powder or zeolite powder made by containing the natural antimicrobial agent according to the present invention can be added together with other additives such as an antifoaming agent to produce a film.

When used in the manufacture of agricultural films, silica powder or zeolite powder made by containing a natural antimicrobial phytoncide and thymol mixture or phytoncide and sparemint mixture in silica is preferably added in an amount of 1 to 70% by weight based on 100% by weight of the resin. . If less than 1% by weight can not exhibit the above functionality sufficiently, in the range exceeding 70% by weight it is not possible to maintain the light transmittance or mechanical strength required as an agricultural film. If it is made of an agricultural film, polyethylene, ethylene / vinyl acetate copolymer and the like can be exemplified as the resin.

In addition, when the present invention is made of a film for packaging, polyethylene (LLPE, LDPE, etc.), polypropylene, ethylene / vinyl alcohol copolymer, ethylene / vinylacetate copolymer, etc. may be exemplified as such a resin.

When the natural antimicrobial agent according to the present invention is coated and applied to the film, in order to add lubrication and water repellency, fatty acids such as methylene stearic acid, oleic acid and palmitic acid, metal salts thereof, fatty acid amides derived from fatty acids, polyethylene wax, etc. Waxes, esters such as liquid paraffin, glycerin fatty acid esters, higher alcohols and the like can be added.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the invention as defined in the appended claims. You will understand.

1 distillation tank
2 cooling system
3 oil-water separator
4 steam generator

Claims (12)

delete delete delete delete delete delete delete delete Natural antibacterial agents comprising phytoncide and thymol or phytoncide and spearmint,
Biodegradable starch mixed with the natural antimicrobial agent to increase the sustained release of the natural antimicrobial agent
Including,
The natural antimicrobial agent comprising the phytoncide and thymol is included in the total active ingredient 4 to 30% by weight of the thymol, 70 to 96% by weight of the phytoncide,
The natural antimicrobial agent comprising the phytoncide and spearmint contains 4 to 30% by weight of the spiramine in total active ingredients, and the phytoncide is 70 to 96% by weight of the disease prevention material of fruit. The method of claim 9,
The natural antibacterial agent and biodegradable starch mixture is a micro-encapsulated fruit disease prevention material. A natural antimicrobial agent comprising phytoncide and thymol or phytoncide and spearmint,
The natural antimicrobial agent comprising the phytoncide and thymol is included in the total active ingredient 4 to 30% by weight of the thymol, 70 to 96% by weight of the phytoncide,
The natural antimicrobial agent including the phytoncide and spearmint contains 4 to 30% by weight of the spiamine in the total active ingredients, and the phytoncide is 70 to 96% by weight of the fruit bag. 12. The method of claim 11,
The natural antimicrobial agent is mixed with a water-repellent liquid is coated on a base for fruit bags, or a fruit bag separately spray-coated on a base for fruit bags.

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