KR101218178B1 - Time Resolved Fluorescence Module and Immunoassay Method Using the Same - Google Patents

Abstract

Time resolved fluorescence modules are provided. This module comprises a microfluidic chip, a microfluidic chip, comprising a sensing part having at least one fluorescent particle-biomaterial complex that specifically binds to the biological sample to form a fluorescent particle-biomaterial complex for detection of the biological sample. A light source for providing fluorescence to generate fluorescence from the fluorescent particle-biobond material complex, a detector for detecting fluorescence generated from the microfluidic chip, and a light for preventing light from the light source from directly entering the detector It includes a block. The microfluidic chip is moved in one direction, and the light source and the detector are alternately turned on / off by the movement and the light blocking unit of the microfluidic chip.

Description

Time Resolved Fluorescence Module and Immunoassay Method Using the Same}

The present invention relates to a time resolved fluorescence module and an immunoassay method using the same, and more particularly, to an auto time resolved fluorescence (auto time resolved fluorescence (auto TRF) module and an immunoassay method using the same.

Test methods used for diagnosing diseases are mainly based on color development, fluorescence, etc. by enzyme reaction, but recently, immunoassay using an immune response between an antigen and an antibody has also been used. Such immunoassay methods are mainly labeled biosensors in which antibodies are labeled with radioisotopes or fluorescent substances to determine the presence or absence of antigens and quantified by the intensity of radiation or fluorescence.

Conventional immunoassay methods measure signals obtained by labeling antigens or antibodies with radioactive substances, luminescent substances or fluorescent substances, Enzyme Linked Immunosorbent Assays (ELISAs) and Western blotting that combine photolabels with catalytic reactions of enzymes. Optical measurement methods such as (Western blotting) are the most used. These methods require complex procedures that can be performed primarily by laboratory-based skilled researchers, and the devices for analysis are expensive, large-scale devices, and require long analysis time.

Antibodies, such as the target substance of the immune sensor, are present in very low concentrations in biological samples such as whole blood, serum, urine, etc., and therefore, the immune sensor is more sensitive than the biosensor technology of detecting other substances. It should be equipped with highly sensitive signaling technology which is much better in terms of detection limit. In addition, since antibodies, protein antigens, and proteins are easily changed in structure due to changes in the external environment, the recognition site of the antigen or antibody is deteriorated, and thus, it is easy to lose the intrinsic biometric function. In the condition of the immune sensor to be analyzed in the solid form, the fabrication of a sensor surface suitable for the biological materials that can maintain the activity of these biological materials, the immobilization technology of the biological materials to raise the detection limit, and the biometric response are quantified signals. It is necessary to secure the measuring method to be switched.

Rapid diagnostic test kits for immunoassays (hereafter referred to as rapid diagnostic test kits) are point-of-tests that allow for diagnostic testing using biological samples such as blood, urine, and saliva. Inspection tool for care. Examples of such rapid diagnostic test kits include pregnancy diagnosis kits and AIDS diagnosis kits.

Such a diagnostic device must establish a method capable of detecting a predetermined biomaterial (protein or DNA, etc.) for diagnosis. Background Art A fluorescent labeling method using an organic dye or the like has been known as a conventional method for detecting a biological material. The fluorescent label emits various colors depending on the type to provide a detection means for the target biomaterial.

Meanwhile, when a plurality of biological materials are to be detected at the same time, a plurality of fluorescent labels emitting light with different colors are required. However, when a plurality of colors emit light at the same time, photobleaching may occur. In addition, the conventional fluorescent label has a disadvantage of having optically narrow excitation and emission band width, and when combined with the biomaterial, may adversely affect the activity of the biomaterial. There are many limitations such as.

The problem to be solved by the present invention is an automatic time-division that can perform the analysis without adjustments to the light source and detector in the time resolved fluorescence module for qualitatively and quantitatively analyzing the infection and the degree of infection of the infectious disease contained in the biological sample. Solution to provide a fluorescent module.

Another object of the present invention is to provide an immunoassay diagnostic method using an automated time resolved fluorescence module that can qualitatively and quantitatively analyze the presence and extent of infection of an infectious disease included in a biological sample.

The problem to be solved by the present invention is not limited to the above-mentioned problems, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.

In order to achieve the above object, the present invention provides a time resolved fluorescent module. This module comprises a microfluidic chip, a microfluidic chip, comprising a sensing part having at least one fluorescent particle-biomaterial complex that specifically binds to the biological sample to form a fluorescent particle-biomaterial complex for detection of the biological sample. A light source for providing fluorescence to generate fluorescence from the fluorescent particle-biobond material complex, a detector for detecting fluorescence generated from the microfluidic chip, and a light for preventing light from the light source from directly entering the detector It may include a block. The microfluidic chip may be moved in one direction, and the light source and the detector may be alternately turned on / off by the movement and the light blocking unit of the microfluidic chip.

The light blocking unit has a first light blocking plate having a plurality of pinholes disposed between the light source and the microfluidic chip for passing light and a second light having a plurality of pinholes disposed between the microfluidic chip and the detector for passing fluorescence It may include a blocking plate. The first light blocking plate may be spaced apart from the sensing part of the microfluidic chip in one direction, and the second light blocking plate may be spaced apart from the sensing part of the microfluidic chip in one direction to face the first light blocking plate.

The pinholes of the first and second light blocking plates may be spaced apart from each other to have a range of 1 to 50 μm in one direction.

The width of the first and second light blocking plates may range from 1 to 50 mm.

The pinholes of the first and second light blocking plates may have a diameter in the range of 15 μm to 150 μm.

The first and second light blocking plates may be about 100 μm away from the microfluidic chip in a direction perpendicular to one direction.

The light source is arranged to provide light inclined with respect to the microfluidic chip, the light blocker is disposed on one side of the microfluidic chip in a direction perpendicular to one direction, and the detector is symmetrical in a direction perpendicular to one direction with respect to the light source. It is disposed, it can detect the fluorescence generated from the microfluidic chip. The light blocking unit may be about 100 μm away from the microfluidic chip in a direction perpendicular to one direction.

The sensing unit of the microfluidic chip may have two or more kinds of fluorescent particle-biomaterial complexes.

Fluorescent particle-biomaterial composites include gas phase condensation, high frequency plasma chemical synthesis, chemical precipitation, hydrothermal synthesis, electrical dispersion reaction, combustion synthesis, sol-gel synthesis, thermochemical synthesis, microfluidizer process, microemulsion technique and high It can be formed by at least one manner selected from among energy mechanical milling.

The biomaterial of the fluorescent nanoparticle-biomaterial complex may include at least one selected from nucleic acids, amino acids, fats, glycoproteins, and antibodies, including DNA or RNA.

If the biological sample that specifically binds to the fluorescent nanoparticle-biomaterial complex is an antigen, the biomaterial may be an antibody.

The light source may be a light emitting diode.

The apparatus may further include a first lens disposed between the light source and the microfluidic chip to focus light onto the microfluidic chip.

The detector may be a silicon detector, a csid photosensitive device or a CMOS photosensitive device.

The apparatus may further include a second lens disposed between the microfluidic chip and the detector to focus or diverge fluorescence generated from the microfluidic chip.

The detector is a silicon detector, and the second lens can focus fluorescence generated from the microfluidic chip.

The movement of the microfluidic chip moves at a constant speed in one direction, and the speed may range from 0.5 to 2 m / s.

The detector is a cdsi photosensitive device or a CMOS photosensitive device, and the second lens can emit fluorescence generated from the microfluidic chip.

The movement of the microfluidic chip is a reciprocating vibration in one direction, and the reciprocating vibration may have an amplitude in the range of 50 μm to 200 μm.

In addition, in order to achieve the above another object, the present invention provides an immunoassay diagnostic method. The method injects a biological sample into a microfluidic chip including a sensing unit having at least one fluorescent particle-biomaterial complex, thereby specifically binding the biological sample to the fluorescent particle-biological complexes in the sensing unit. Forming biocomposite complexes, providing light to the microfluidic chip to generate fluorescence from the fluorescent particle-bioconjugate complexes, and detecting fluorescence generated from the microfluidic chip. The microfluidic chip moves in one direction, and light is periodically provided to the sensing part of the microfluidic chip by the movement of the microfluidic chip, and fluorescence is periodically detected by the movement of the microfluidic chip and the light This may be prevented from being detected directly.

The movement of the microfluidic chip moves at a constant speed in one direction, and the speed may range from 0.5 to 2 m / s.

The movement of the microfluidic chip is a reciprocating vibration in one direction, and the reciprocating vibration may have an amplitude in the range of 50 μm to 200 μm.

The sensing unit of the microfluidic chip may have two or more kinds of fluorescent particle-biomaterial complexes.

The biomaterial of the fluorescent nanoparticle-biomaterial complex may include at least one selected from nucleic acids, amino acids, fats, glycoproteins, and antibodies, including DNA or RNA.

If the biological sample that specifically binds to the fluorescent nanoparticle-biomaterial complex is an antigen, the biomaterial may be an antibody.

As described above, according to the problem solving means of the present invention, by using the movement movement and the light blocking unit of the microfluidic chip, adjustment of the light source and the detector for detecting the infectious disease included in the biological sample is not required. Accordingly, a time resolved fluorescence module that does not require a complicated circuit configuration can be provided.

In addition, according to the solution of the problem of the present invention by using the movement and light block of the microfluidic chip. No adjustments to light sources and detectors for detecting infectious diseases included in biological samples are required. Accordingly, an immunoassay diagnostic method capable of omitting complicated analysis conditions for qualitatively and quantitatively analyzing an infectious disease included in a biological sample can be provided.

1 is a schematic diagram illustrating a configuration of a time resolved fluorescent module according to an embodiment of the present invention;
2 is an enlarged view illustrating an enlarged portion A of FIG. 1;
3 is a schematic diagram illustrating a time resolved fluorescence module according to another embodiment of the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS The advantages and features of the present invention and the manner of achieving them will become apparent with reference to the embodiments described in detail below with reference to the accompanying drawings. However, the present invention is not limited to the embodiments described herein but may be embodied in different forms. Rather, the embodiments disclosed herein are provided so that the disclosure can be thorough and complete, and will fully convey the concept of the invention to those skilled in the art, and the invention is only defined by the scope of the claims. Like reference numerals refer to like elements throughout the specification.

The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. In the present specification, the singular form includes plural forms unless otherwise specified in the specification. It is to be understood that the terms 'comprises' and / or 'comprising' as used herein mean that an element, step, operation, and / or apparatus is referred to as being present in the presence of one or more other elements, Or additions. In addition, since they are in accordance with the preferred embodiment, the reference numerals presented in the order of description are not necessarily limited to the order.

In addition, the embodiments described herein will be described with reference to cross-sectional views and / or plan views, which are ideal illustrations of the present invention. In the drawings, the size and / or thickness of the components are exaggerated for the effective description of the technical content. Thus, the shape of the illustrations may be modified by manufacturing techniques and / or tolerances. Accordingly, the embodiments of the present invention are not limited to the specific forms shown, but also include variations in forms generated by the manufacturing process. Accordingly, the components illustrated in the figures have schematic attributes, and the appearance of the components illustrated in the figures is intended to illustrate a particular form of component of the apparatus and is not intended to limit the scope of the invention.

FIG. 1 is a schematic diagram illustrating a configuration of a time resolved fluorescence module according to an exemplary embodiment of the present invention, and FIG. 2 is an enlarged view illustrating an enlarged portion A of FIG. 1.

1 and 2, the time resolved fluorescence module 100 includes a microfluidic chip 130, a light source 110, a detector 140, and light blocking parts 120a and 120b.

The microfluidic chip 130 may include a sensing unit having fluorescent particle-biomaterial complexes for detecting an infectious disease included in a biological sample. The fluorescent particle-biomaterial complex may be specifically combined with an infectious disease included in a biological sample to form a fluorescent particle-biomaterial complex. The microfluidic chip 130 may move in one direction (both arrows).

The biomaterial of the fluorescent particle-biomaterial complex may include at least one selected from nucleic acid, amino acid, fat, glycoprotein, and antibody including DNA or RNA. Can be.

Fluorescent particle-biomaterial complexes include gas phase condensation method, high frequency plasma chemical synthesis method, conventional chemical precipitation, hydrothermal synthesis method, and electrical dispersion reaction. Electric dispersion re-action method, combustive synthesis method, sol-gel synthesis method, thermochemical synthesis method, microfludizer process, microemulsion It may be formed by at least one method selected from microemulson technology and high energy mechanical milling.

When the infectious disease included in the biological sample specifically binding to the fluorescent particle-biomaterial complex is an antigen, the biomaterial of the fluorescent particle-biomaterial complex may be an antibody.

The sensing unit of the microfluidic chip 130 may have two or more kinds of fluorescent particle-biomaterial complexes. Accordingly, the microfluidic chip 130 may be used to simultaneously detect a plurality of infectious diseases included in the biological sample.

The microfluidic chip 130 may be capable of various operations such as mixing, separating, and replacing a biological sample in a fluid form, that is, a biological sample such as blood, and the like, moving, stopping, changing a speed, and other fluid such as a test solution. The microfluidic chip 130 is a type of polymer material used as a material, a type of fluid used for detection, a contact angle, and the like, such as a width, depth, and length of a fluid channel, which are variables that may affect the flow of the fluid. It may be implemented in consideration of such. In addition, the microfluidic chip 130 may be implemented in consideration of variables such as types and methods of pumps and valves for efficient transport of fluids, and locations of the microfluidic chips 130.

The biological sample collected from the infected patient to be diagnosed is injected into the microfluidic chip 130 without pretreatment, and the biological sample and the reaction solution mixture move in the fluid channel while infecting the infectious disease and fluorescent particle-biomaterial complex included in the biological sample. The physical conjugation of is formed.

The light source 110 may continuously provide light to the microfluidic chip 130. The light provided by the light source 110 may excite the fluorescent particles of the fluorescent particle-biobinding material complex of the microfluidic chip 130 to generate fluorescence. The light source 110 may include a high brightness light emitting diode (LED).

The apparatus may further include a first lens 115 disposed between the light source 110 and the microfluidic chip 130. The first lens 115 may be used to focus light onto the microfluidic chip 130.

When the microfluidic chip 130 has two or more kinds of fluorescent particle-biomaterial complexes, different fluorescences may be generated by excitation of fluorescent particles of two or more kinds of fluorescent particle-biomaterial complexes. Accordingly, fluorescence may occur for each of the plurality of infectious diseases included in the biological sample.

As described above, the detector 140 may continuously detect fluorescence generated by excitation of the fluorescent particles of the fluorescent particle-biobinding material complex of the microfluidic chip 130. The detector 140 may be a silicon detector, a CCD photosensitive device, or a CMOS photosensitive device.

It may further include a second lens 135 disposed between the detector 140 and the microfluidic chip 130. The second lens 115 may be for focusing or diverging fluorescence generated from the microfluidic chip 130.

The light blocking parts 120a and 120b are disposed between the light source 110 and the microfluidic chip 130 and have a first light blocking plate 120a and a microfluidic chip having a plurality of pinholes for passing light. The second light blocking plate 120b may be disposed between the 130 and the detector 140 and have a plurality of pinholes for passing fluorescence. That is, the cross-sections of the first and second light blocking plates 120a and 120b may have a shape of the teeth of a comb by a plurality of pinholes. The first and second light blocking plates 120a and 120b may have a width in a range of 1 to 50 mm. This is because the silicon detector, which is used as the detector 140, has a detection area of about 1 mm, and the Sisid photosensitive device or CMOS photosensitive device has a detection area in the range of about 10-50 mm.

The plurality of pinholes of the first light blocking plate 120a are spaced apart from the sensing unit of the microfluidic chip 130 in one direction, and the plurality of pinholes of the second light blocking plate 120b are the microfluidic chip 130. The sensing unit may be spaced apart to face the plurality of pinholes of the first light blocking plate 120a in one direction. The pinholes of the first and second light blocking plates 120a and 120b may be spaced apart from each other in a range of about 1 to 50 μm in one direction. Accordingly, the light provided from the light source 110 can be prevented from directly entering the detector 140 by the light blocking units 120a and 120b.

The first and second light blocking plates 120a and 120b may be spaced apart from the microfluidic chip 130 by about 100 μm in a direction perpendicular to one direction (d, e). This may be to eliminate friction that may occur between the microfluidic chip 130 and the light blocking parts 120a and 120b by the movement of the microfluidic chip 130. The pinholes of the first and second light blocking plates 120a and 120b may have diameters a and b in a range of about 15 to 150 μm. That is, the first and second light blocking plates 120a and 120b may have about 10 to 1,000 pinholes in one direction.

When the silicon detector is used as the detector 140, the movement of the microfluidic chip 130 may be a movement G at a constant speed in one direction. This is because the silicon detector is a point detector. The speed of the movement of the microfluidic chip 130 may range from about 0.5 to 2 m / s. In this case, the second lens 135 may focus the fluorescence generated from the microfluidic chip 130.

On the contrary, when the CD photosensitive device or the CMOS photosensitive device is used as the detector 140, the movement of the microfluidic chip 130 may be a reciprocating vibration motion G in one direction. This is because the CSI photosensitive element and the CMOS photosensitive element are area detectors. The reciprocating vibration movement G of the microfluidic chip 130 may have a frequency of about 10 kHz and an amplitude of about 50 to 200 μm. In this case, the second lens 135 may emit fluorescence generated from the microfluidic chip 130.

The movement of the microfluidic chip 130 and the light blocking unit 120a or 120b may have an effect of alternately turning on / off the light source 110 and the detector 140. That is, the time-resolved fluorescence method may be performed by the movement of the microfluidic chip 130 and the light blocking units 120a and 120b. Accordingly, the time resolved fluorescent module 100 according to the embodiment of the present invention may be a transmission type automatic time resolved fluorescent module.

When the microfluidic chip 130 has two or more kinds of fluorescent particle-biomaterial complexes, different fluorescences may be generated by excitation of the fluorescent particles of each of the two or more kinds of fluorescent particle-biomaterial complexes. Since each of these different fluorescences have different wavelengths, they may be detected separately in the detector 140.

The detector 140 may analyze wavelengths of fluorescence and analyze types of infectious diseases included in biological samples corresponding to wavelengths of fluorescence and the extent of infectious diseases. Accordingly, qualitative and quantitative analysis of each of the plurality of infectious diseases included in the biological sample may be possible. In addition, it may be possible to simultaneously analyze a plurality of infectious diseases included in a biological sample. Accordingly, the time resolved fluorescence module 100 can qualitatively and quantitatively identify whether each of the plurality of infectious diseases included in the biological sample is infected and the degree of infection at the same time.

3 is a schematic diagram illustrating a time resolved fluorescence module according to another embodiment of the present invention. For simplicity, a detailed description of components similar to those described through the time resolved fluorescence module according to the above-described embodiments of FIGS. 1 and 2 will be omitted.

Referring to FIG. 3, the time resolved fluorescent module 200 includes a microfluidic chip 230, a light source 210, a detector 240, and a light blocking unit 220.

The microfluidic chip 230 may include a sensing unit having fluorescent particle-biomaterial complexes for detecting an infectious disease included in a biological sample. The fluorescent particle-biomaterial complex may be specifically combined with an infectious disease included in a biological sample to form a fluorescent particle-biomaterial complex. The microfluidic chip 230 may move in one direction (both arrows).

The sensing unit of the microfluidic chip 230 may have two or more kinds of fluorescent particle-biomaterial complexes. Accordingly, the microfluidic chip 230 may be used to simultaneously detect a plurality of infectious diseases included in the biological sample.

The light source 210 may continuously provide light to the microfluidic chip 130. The light provided by the light source 210 may excite the fluorescent particles of the fluorescent particle-biobinding material complex of the microfluidic chip 230 to generate fluorescence.

The lens may further include a first lens 215 disposed between the light source 210 and the microfluidic chip 230. The first lens 215 may be used to focus light onto the microfluidic chip 230.

When the microfluidic chip 230 has two or more kinds of fluorescent particle-biomaterial complexes, different fluorescences may be generated by excitation of fluorescent particles of two or more kinds of fluorescent particle-biomaterial complexes. Accordingly, fluorescence may occur for each of the plurality of infectious diseases included in the biological sample.

As described above, the detector 240 may continuously detect fluorescence generated by excitation of the fluorescent particles of the fluorescent particle-biobinding complex of the microfluidic chip 230. The detector 240 may be a silicon detector, a Sisid photosensitive device, or a CMOS photosensitive device.

It may further include a second lens 235 disposed between the detector 240 and the microfluidic chip 230. The second lens 215 may be for focusing or diverging fluorescence generated from the microfluidic chip 230.

The light blocking unit 220 may be disposed on one side of the microfluidic chip 230 in a direction perpendicular to one direction. The light source 210 may be disposed to provide light inclined with respect to the microfluidic chip 230, and the detector 240 may be disposed to be symmetrical in a direction perpendicular to the one direction with respect to the light source 210. Accordingly, the light provided from the light source 210 may be prevented from directly entering the detector 240 by the light blocking unit 220.

The light blocking unit 220 may be spaced apart from about 100 μm in a direction perpendicular to the microfluidic chip 230 in one direction (see FIG. 2D). This may be to eliminate friction that may occur between the microfluidic chip 230 and the light blocking unit 220 by the movement of the microfluidic chip 230.

When the silicon detector is used as the detector 240, the movement of the microfluidic chip 230 may be moving at a constant speed in one direction (see G of FIG. 2). This is because the silicon detector is a point detector. The speed of the movement of the microfluidic chip 230 may range from about 0.5 to 2 m / s. In this case, the second lens 235 may focus the fluorescence generated from the microfluidic chip 230.

On the contrary, when the CD photosensitive device or the CMOS photosensitive device is used as the detector 240, the movement of the microfluidic chip 230 may be a reciprocating vibration motion in one direction (see FIG. 2G). This is because the sisid photosensitive element and the CMOS photosensitive element are area detectors. The reciprocating vibration movement of the microfluidic chip 230 may have a frequency of about 10 kHz and an amplitude of about 50 to 200 μm. In this case, the second lens 235 may emit fluorescence generated from the microfluidic chip 230.

The movement of the microfluidic chip 230 and the light blocking unit 220 may alternately turn on / off the light source 210 and the detector 240. That is, time-resolved fluorescence may be performed by the movement of the microfluidic chip 230 and the light blocking unit 220. Accordingly, the time resolved fluorescent module 200 according to the embodiment of the present invention may be a reflective automatic time resolved fluorescent module.

When the microfluidic chip 230 has two or more kinds of fluorescent particle-biomaterial complexes, different fluorescences may be generated by excitation of the fluorescent particles of each of the two or more kinds of fluorescent particle-biomaterial complexes. Since each of these different fluorescences have different wavelengths, they may be detected separately in the detector 240.

The detector 240 may analyze wavelengths of fluorescence to analyze the types of infectious diseases included in the biological samples corresponding to the wavelengths of the fluorescence and the extent of the infectious diseases. Accordingly, qualitative and quantitative analysis of each of the plurality of infectious diseases included in the biological sample may be possible. In addition, it may be possible to simultaneously analyze a plurality of infectious diseases included in a biological sample. Accordingly, a time resolved fluorescence module 200 capable of simultaneously and qualitatively and quantitatively determining whether each of the plurality of infectious diseases included in the biological sample is infected and the degree of infection may be provided.

The time-resolved fluorescent module according to the embodiments of the present invention described above does not require adjustment to a light source and a detector for detecting an infectious disease included in a biological sample by using the movement movement and the light blocking unit of the microfluidic chip. Accordingly, the time resolved fluorescent module according to embodiments of the present invention can be used for detection of infectious disease without having a complicated circuit configuration.

In addition, the time-resolved fluorescent module according to the embodiments of the present invention, by using the movement and light block of the microfluidic chip, qualitatively and qualitatively whether or not each of the plurality of infectious diseases included in the biological sample is infected It can be confirmed quantitatively. Accordingly, the time resolved fluorescent module according to embodiments of the present invention can be used to simultaneously and qualitatively and quantitatively identify a plurality of infectious diseases included in a biological sample.

While the present invention has been described in connection with what is presently considered to be practical exemplary embodiments, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, It will be understood. It is therefore to be understood that the above-described embodiments are illustrative and non-restrictive in every respect.

100, 200: time resolved fluorescent module
110, 210: light source
115,215: First lens
120a, 120b, 220: light blocking unit
130, 230: microfluidic chip
135,235: second lens
140, 240: detector

Claims (26)

A microfluidic chip including a sensing unit having at least one fluorescent particle-biomaterial complex which is specifically combined with the biological sample to form a fluorescent particle-biomaterial complex for detecting the biological sample;
A light source for supplying light to the microfluidic chip to generate fluorescence from the fluorescent particle-biological binder composite;
A detector for detecting the fluorescence generated from the microfluidic chip; And
It includes a light blocking portion that serves to prevent the light provided from the light source to enter the detector directly,
The microfluidic chip moves in one direction, and the light source and the detector are alternately turned on / off by the movement and the light blocking unit of the microfluidic chip. Fluorescent module. The method of claim 1,
The light blocking unit:
A first light blocking plate disposed between the light source and the microfluidic chip and having a plurality of pinholes for passing the light; And
A second light blocking plate disposed between the microfluidic chip and the detector, the second light blocking plate having a plurality of pinholes for passing the fluorescence;
The first light blocking plate is spaced apart from the sensing part of the microfluidic chip in the one direction, and the second light blocking plate is the first light blocking plate in the one direction with the sensing part of the microfluidic chip. Time resolved fluorescent module, characterized in that spaced apart to face. The method of claim 2,
And the pinholes of the first and second light blocking plates are spaced apart from each other to have a range of 1 to 50 μm in the one direction. The method of claim 2,
Time-reduced fluorescent module, characterized in that the width of the first and second light blocking plate has a range of 1 ~ 50 mm. The method of claim 2,
And the pinholes of the first and second light blocking plates have a diameter in a range of 15 to 150 μm. The method of claim 2,
And the first and second light blocking plates are spaced apart from each other in the direction perpendicular to the one direction from the microfluidic chip. The method of claim 1,
The light source is arranged to provide the light inclined with respect to the microfluidic chip,
The light blocking unit is disposed on one side of the microfluidic chip in a direction perpendicular to the one direction, and
The detector is disposed to be symmetrical in a direction perpendicular to the one direction with respect to the light source, the time-resolved fluorescent module, characterized in that for detecting the fluorescence generated from the microfluidic chip. 8. The method of claim 7,
And the light blocking unit is spaced apart from the microfluidic chip in a direction perpendicular to the one direction. The method of claim 1,
And the sensing unit of the microfluidic chip has two or more kinds of fluorescent particle-biomaterial complexes. The method of claim 1,
The fluorescent particle-biomaterial composite may include gas phase condensation, high frequency plasma chemical synthesis, chemical precipitation, hydrothermal synthesis, electrical dispersion reaction, combustion synthesis, sol-gel synthesis, thermochemical synthesis, microfluidizer process, microemulsion technique, and Time resolved fluorescent module, characterized in that formed by at least one method selected from high energy mechanical milling. The method of claim 1,
The biomaterial of the fluorescent particle-biomaterial complex comprises at least one selected from nucleic acids, amino acids, fats, glycoproteins, and antibodies including DNA or RNA. 12. The method of claim 11,
When the biological sample that specifically binds to the fluorescent particle-biomaterial complex is an antigen, the biodegradable fluorescent module, characterized in that the biomaterial is an antibody. The method of claim 1,
The light source is a time-resolved fluorescent module, characterized in that the light emitting diode. The method of claim 1,
And a first lens disposed between the light source and the microfluidic chip to focus the light onto the microfluidic chip. The method of claim 1,
The detector is a time-resolved fluorescence module, characterized in that the silicon detector, a photosensitive device or a CMOS photosensitive device. The method of claim 1,
And a second lens disposed between the microfluidic chip and the detector to focus or diverge the fluorescence generated from the microfluidic chip. 17. The method of claim 16,
The detector is a silicon detector,
And the second lens focuses the fluorescence generated from the microfluidic chip. 18. The method of claim 17,
The movement of the microfluidic chip is moving at a constant speed in the one direction,
Time velocity fluorescence module, characterized in that having a range of 0.5 ~ 2 m / s. 17. The method of claim 16,
The detector is a ssid photosensitive device or a CMOS photosensitive device,
And the second lens emits the fluorescence generated from the microfluidic chip. 20. The method of claim 19,
The movement movement of the microfluidic chip is a reciprocating vibration movement in the one direction,
The reciprocating vibration motion is a time resolved fluorescence module, characterized in that having an amplitude in the range of 50 ~ 200 μm. Injecting a biological sample into a microfluidic chip including a sensing unit having at least one fluorescent particle-biomaterial complex, thereby specifically binding the biosample to the fluorescent particle-biomaterial complexes, thereby detecting fluorescent nanoparticles- Forming biobinding complexes;
Providing light to the microfluidic chip to generate fluorescence from the fluorescent particle-biobinding complexes; And
Detecting the fluorescence generated from the microfluidic chip,
The microfluidic chip moves in one direction,
The light is periodically provided to the sensing unit of the microfluidic chip by the movement of the microfluidic chip, and
The fluorescence is periodically detected by the movement of the microfluidic chip,
Immunoassay diagnostic method, characterized in that the light is prevented from being detected directly. 22. The method of claim 21,
The movement of the microfluidic chip is moving at a constant speed in the one direction,
The speed is immunoassay diagnostic method, characterized in that it has a range of 0.5 ~ 2 m / s. 22. The method of claim 21,
The movement movement of the microfluidic chip is a reciprocating vibration movement in the one direction,
The reciprocating vibration movement is immunoassay diagnostic method, characterized in that having an amplitude in the range of 50 ~ 200 μm. 22. The method of claim 21,
And the sensing unit of the microfluidic chip has two or more kinds of fluorescent particle-biomaterial complexes. 22. The method of claim 21,
The biomaterial of the fluorescent nanoparticle-biomaterial complex comprises at least one selected from nucleic acids, amino acids, fats, glycoproteins, and antibodies including DNA or RNA. 26. The method of claim 25,
If the biological sample that specifically binds to the fluorescent nanoparticle-biomaterial complex is an antigen, the bioassay diagnostic method, characterized in that the antibody.

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