KR101216589B1

KR101216589B1 - Portable Nano-paticles Exposure Chamber for In-vitro Type Testing Toxicity of Nano-paticles

Info

Publication number: KR101216589B1
Application number: KR1020100126528A
Authority: KR
Inventors: 권용택; 윤진욱; 전기수; 조재호; 이재성; 서기원
Original assignee: (주)에이치시티
Priority date: 2010-12-10
Filing date: 2010-12-10
Publication date: 2012-12-31
Also published as: KR20120065162A

Abstract

The present invention relates to a portable nanoparticle exposure apparatus, which can be carried and transported as an independent apparatus without a separate particle generator or air circulation facility, and is configured to expose nanoparticles generated in a real field to a test object. Toxicity evaluation test on the actual nanoparticles to be performed can be performed accurately and conveniently, and the test case is vacuum-compressed in such a manner that the tissue cells of the experimental animal are placed inside the housing and the tissue cells are wrapped. By exposing the nanoparticles to the test subject, the toxicity test for nanoparticles can be conveniently and conveniently performed by exposing the nanoparticles to tissue cells of the experimental animal rather than by injecting the respiratory organs of the experimental animal. The nanoparticles are introduced to the test object through the particle injection tube of the case. By intensive uniformly exposed portion without leakage, the more accurate and nanoparticles Toxicity test The portable nanoparticles exposure apparatus of a number of ways.

Description

Portable Nano-paticles Exposure Chamber for In-vitro Type Testing Toxicity of Nano-paticles

The present invention relates to a portable nanoparticle exposure apparatus. More specifically, it is possible to carry and transport it as an independent device without a separate particle generator or air circulation facility, and to configure the nanoparticles generated in the actual field to be exposed to the test object. Toxicology assessment tests can be performed accurately and conveniently, and the test case is vacuum compressed to expose the nanoparticles to the test subject in an airtight manner by placing the tissue cells of the experimental animal inside the housing and surrounding the tissue cells. In addition, the toxicity test for nanoparticles can be easily and conveniently performed by exposing the nanoparticles to the tissue cells of the experimental animal rather than through the respiratory organ of the experimental animal. Concentrates and uniformly distributes nanoparticles to an object without external leakage Ex thereby, to a more accurate and various ways of nanoparticles Toxicity test The portable nanoparticles exposure apparatus.

If the 20th century was a micro era, the 21st century could be called the nano era. Nanotechnology can be classified into nanomaterials, nanodevices, and environmental and biotechnology-based technologies according to their applications.

These nanotechnologies artificially manipulate microscopic materials at the atomic or molecular level to create materials or devices with new properties and functions, which are the basis for information technology (IT) and other biotechnology (BT) technologies today. It is hailed as a cutting-edge technology for realizing).

However, while nanotechnology offers many benefits and benefits that can be recognized as a new technological revolution throughout the industry, it is also well known that there are potential risks. This is due to the nature of nanotechnology.

In other words, the smaller the particles, the larger the specific surface area ratio, and the smaller the larger the specific surface area ratio, the greater the toxicity when reacting with biological tissues. For example, some nanoparticles such as titanium dioxide, carbon powder, diesel particles, etc. It has already been found in academic experiments that the smaller the size, the stronger the toxicity. In addition, ultra-fine nanoparticles can be lodged deep into the alveoli or migrate to the brain without being trapped by the airways or mucous membranes. Furthermore, recent studies have reported that the accumulation of nanoparticles in the body causes diseases or central nervous system disorders. .

Therefore, in recent years, with the development of nanotechnology, stability evaluation of nanotechnology has been actively progressed. For example, nanoparticle inhalation toxicity evaluation tests that evaluate the toxicity generated when nanoparticles are inhaled and accumulated in the human body have various experiments. Animals are being studied. The human hazard data obtained through the nanoparticle inhalation toxicity evaluation test is used as various basic data on nanoparticles throughout the industry such as nanofibers, cosmetics, semiconductors, and drug carriers.

Inhalation toxicity evaluation test for these nanoparticles generally generates nanoparticles in an aerosol state and supplies them to a nanoparticle exposure apparatus having a predetermined size suction chamber, and puts an experimental animal into the suction chamber of the nanoparticle exposure apparatus. After exposing the animals to inhale the nanoparticles through the respiratory system, the progress is made by measuring various changes in the experimental animals.

As described above, a test performed in the body of a test animal, which is a test subject, is called an in-vivo test, and a test is performed by adjusting a condition in a separate test tube by dissecting or culturing some tissue cells of the test subject. It is called an in-vitro test. In recent years, legislation regulating the testing of living organisms has been strengthened, especially in OECD countries, and in-vitro rather than in-vivo testing is more effective in protecting animals. It is required.

However, since the conventional nanoparticle exposure apparatus according to the prior art is configured in such a way that the nanoparticles are directly injected through the respirator of the experimental animal, all animals that have been put into the experiment should be killed when the experiment is completed. In addition to the moral problems, there was a problem in that the cost of purchasing or killing an experimental animal was further increased, resulting in a significant increase in the cost of the experiment as a whole.

In addition, the conventional nanoparticle exposure apparatus according to the prior art requires a separate particle generator and must have a significant size of the nanoparticle exposure chamber and air circulation facilities, so that the scale and the installation and operation costs are very expensive, The test was carried out only at research institutes, but the problem was that these tests were not widely carried out in small laboratories such as university laboratories. In particular, since the toxicity test for the nanoparticles is not performed in the actual site exposed to the nanoparticles, but in a separate laboratory, the accuracy and reliability of the toxicity test for the nanoparticles occurring in the actual site is degraded. There was.

The present invention has been invented to solve the problems of the prior art, an object of the present invention is to carry and transport as an independent device without a separate particle generator or air circulation facility, the nano-particles generated in the actual field to the test object By configuring the exposure, it is possible to provide a portable nanoparticle exposure device that can accurately and conveniently perform toxicity evaluation tests on actual nanoparticles to be measured.

Another object of the present invention is to expose the nanoparticles to the test subject in an airtight manner by vacuum compressing the test case in a manner in which the tissue cells of the experimental animal are placed inside the housing and surrounding the tissue cells, thereby exposing the nanoparticles to the test animal's respiratory organs. It is possible to carry out simple and convenient toxicity test for nanoparticles by exposing to tissue cells of experimental animals rather than by injection method, and thus, portable nanoparticles that can effectively respond to experimental regulation methods for living things. It is to provide an exposure apparatus.

Still another object of the present invention is to provide a more accurate and versatile method by intensively and uniformly exposing the nanoparticles to the test object without external leakage through the particle injection tube while the test case inside the housing is vacuum compressed to surround the test object. The present invention provides a portable nanoparticle exposure device capable of testing nanoparticle toxicity evaluation.

The present invention, the test object is seated therein and one side of the housing through which the inlet is formed; And an exposure chamber formed in an open shape on one side thereof, and disposed inside the housing to surround the test object, and on one side, a particle injection port connected to the inlet and an inlet pipe, and a separate vacuum pump and a suction pipe. And a test case coupled to the suction chamber in communication with the exposure chamber, wherein the test case wraps the test object and the exposure chamber is sealed as a vacuum pressure is formed in the exposure chamber through the vacuum pump. At the same time as the vacuum pressing on the inner surface of the housing provides a portable nanoparticle exposure apparatus, characterized in that the nanoparticles floating in the outside of the housing continuously through the particle injection port is introduced.

At this time, an open rubber end of the test case may be combined with a separate rubber packing that can be crimped.

In addition, one side of the test case is connected to the connection block formed in each of the injection passage and the discharge passage communicating with the exposure chamber independently therein, the particle injection port is in communication with one end of the injection passage and the suction The discharge port may be communicatively coupled to one end of the discharge passage, and a separate particle injection tube may be communicatively coupled to the other end of the injection passage so that nanoparticles may flow into the exposure chamber.

In addition, the particle injection tube is coupled to the injection flow path of the connection block and the straight pipe portion is disposed in the same diameter straight toward the open one surface of the test case; And extending into one end of the straight pipe portion and extending toward the open side of the in-vitro test case to expand the diameter.

In addition, a plurality of test cases may be disposed inside the housing, and particle injection ports coupled to each test case may be connected to inlets of the housing through branched inlet pipes, respectively.

In addition, the suction discharge port coupled to the plurality of test cases may be connected to one vacuum pump through each branched suction pipe.

In addition, the suction discharge ports coupled to the plurality of test cases may be connected to separate vacuum pumps through independent suction pipes, respectively.

According to the present invention, it is possible to carry and transport as an independent device without a separate particle generator or air circulation facility, and configured to expose the nano-particles generated in the actual site to the test object, thereby real-world nanoparticles to be measured It is effective to carry out the toxicity evaluation test for the drug accurately and conveniently.

In addition, by injecting the nanoparticles through the respirator of the experimental animal by placing the tissue cells of the experimental animal inside the housing and enclosing the tissue cells, the test case is vacuum compressed to expose the nanoparticles to the test object in an airtight state. In addition, the toxicity test for nanoparticles can be easily and conveniently performed by exposing to tissue cells of experimental animals, thereby effectively responding to experimental regulation methods for living organisms.

In addition, by intensively and uniformly exposing the nanoparticles to the test object without external leakage through the particle injection tube while the test case inside the housing is vacuum-compressed to surround the test object, more accurate and various methods of nanoparticle toxicity evaluation test This has a possible effect.

In addition, by allowing the test case to wrap around the test object and to vacuum the housing to prevent external loss of the nanoparticles, the flow rate of the nanoparticles exposed to the test object can be precisely controlled, and most of the nanoparticles are tested through the particle injection tube. By being exposed to the subject, there is an effect that can more accurately measure the effect of the test subject on the exposure of the nanoparticles.

1 is a partial exploded perspective view schematically showing the configuration of a portable nanoparticle exposure apparatus according to an embodiment of the present invention,
2 is a cross-sectional view schematically showing the internal structure of the portable nanoparticle exposure apparatus according to an embodiment of the present invention;
3 is a cross-sectional view schematically showing an internal structure of a test case of a portable nanoparticle exposure apparatus according to an embodiment of the present invention;
4 and 5 are diagrams showing the experimental analysis of the flow of nanoparticles injected into the test case of the portable nanoparticle exposure apparatus according to an embodiment of the present invention,
FIG. 6 is a cross-sectional view schematically illustrating a state in which an internal structure and a protrusion height of a particle injection tube are changed in a test case of a portable nanoparticle exposure apparatus according to another embodiment of the present invention; FIG.
FIG. 7 is a partially exploded perspective view schematically showing the configuration of a portable nanoparticle exposure apparatus according to another embodiment of the present invention; FIG.
FIG. 8 is a cross-sectional view schematically illustrating an internal structure of the portable nanoparticle exposure apparatus illustrated in FIG. 7.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. In the drawings, the same reference numerals are used to designate the same or similar components throughout the drawings. In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear.

1 is a partially exploded perspective view schematically showing the configuration of a portable nanoparticle exposure apparatus according to an embodiment of the present invention, Figure 2 schematically shows the internal structure of a portable nanoparticle exposure apparatus according to an embodiment of the present invention 3 is a cross-sectional view schematically illustrating an internal structure of a test case of a portable nanoparticle exposure apparatus according to an embodiment of the present invention.

The portable nanoparticle exposure apparatus according to an embodiment of the present invention is a device for inhaling nanoparticles and exposing the nanoparticles to a test object in the actual site where the nanoparticles are exposed. Rather, it is a device capable of testing in vitro in which some tissue cells of an animal or human are separated to expose nanoparticles to the separated tissue cells, and a housing 200 and a test case disposed inside the housing 200 ( 100).

The housing 200 has an accommodation space formed therein so that the test case 100 can be seated therein, and an inlet 211 is formed at one side thereof so that external nanoparticles can be introduced into the test case 100. At this time, the test object (T) is seated in the form of tissue cells inside the housing 200, the test case 100 is disposed inside the housing 200 to surround the test object (T). Meanwhile, as illustrated in FIGS. 1 and 2, the housing 200 may be separately formed into the housing body 220 and the housing cover 210, and the test object T and the test case may be formed on the housing body 220. 100 may be seated, and the inlet 211 may be formed in the housing cover 210.

The test case 100 is formed to surround the test object T in the housing 200. An exposure chamber C is formed to expose nanoparticles to an internal space, and the exposure chamber C is externally provided. One surface is formed to be open to communicate with. That is, the test test case 100 may be formed in the form of a container having one surface open, and may be formed in a hollow cylindrical shape such that the outer circumferential surface forms a circumferential surface as shown in FIG. The shape may be variously changed. In this case, in order to smoothly flow the nanoparticles in the exposure chamber C inside the test test case 100, it is preferable to form a hollow cylindrical shape so that stagnant regions are not generated by the corners.

The test case 100 surrounds the test object T and contacts the bottom surface of the housing 200 such that the test object T is openly exposed to the exposure chamber C through an open surface. For example, if the test subject T is a separate tissue cell dissected from the body as shown in FIG. 2, the tissue cell is seated inside the housing 200 in a container such as a chalet, The test case 100 is fixed to the bottom surface of the housing 200 in such a manner as to surround the tissue cells so that the tissue cells as the test subject T are positioned inside the exposure chamber C through an open surface. As described above, the test case 100 surrounding the test object T and fixed to the bottom surface of the housing 200 is fixed in such a manner as to be vacuum-compressed to the housing 200 as the vacuum pressure is formed in the exposure chamber C. This will be described later.

On the other hand, the test case 100 has a particle injection port 110 connected through the inlet 211 and the inlet pipe (P) of the housing 200, and through a separate vacuum pump (R) and the suction pipe (Q) A suction discharge port 120 to be connected is coupled in communication with the exposure chamber (C).

Particle injection port 110 is the inlet port 211 and the inlet pipe so that the nanoparticles floating in the outside atmosphere of the housing 200 can be injected into the exposure chamber (C) of the test case 100 through the inlet port (211) It is connected via (P), in this case, it is preferable that the inlet pipe (P) is formed inclined extension portion (P1) to extend the inner diameter toward the inlet 211 side, through which the more nanoparticles of the outside It may be supplied to the test case 100 in a smooth flow.

Suction discharge port 120 is coupled to the test case 100 in communication with the exposure chamber (C) to suck the exposure chamber (C) space through a separate vacuum pump (R). That is, one end of the suction discharge port 120 is connected to the vacuum pump R through a separate suction pipe Q, and the other end is coupled to communicate with the exposure chamber C of the test case 100. At this time, the vacuum pump (R) is preferably disposed so as to be located outside the housing 200, as shown in FIG.

At this time, the particle injection port 110 and the suction discharge port 120 may be configured to be integrally formed with the test case 100 on one side of the test case 100, as shown in Figures 2 and 3 It is preferably formed in such a way that is coupled to the test case 100 through a separate connection block 130. That is, the connection block 130 is coupled through-sealed to one side of the test case 100, and the injection flow path 131 is in communication with the exposure chamber C of the test case 100 inside the connection block 130. The discharge flow paths 132 are formed independently of each other, and the particle injection port 110 is communicatively coupled to one end of the injection flow path 131, and the suction discharge port 120 is communicatively coupled to one end of the discharge flow path 132. Can be configured. On the other hand, at this time, the other end of the injection passage 131 may be coupled to a separate particle injection tube 140 so that the nanoparticles flow into the exposure chamber (C), the particle injection tube 140 is the test case 100 It may be disposed to protrude in the exposure chamber (C) toward the open one side of the).

Looking in more detail, the connection block 130 is formed in a cylindrical shape, as shown in Figures 1 to 3, there is an injection passage 131 and the discharge passage 132, the injection passage 131 is connected It is formed penetrating from the side surface of the block 130 to the bottom surface, the discharge passage 132 is formed through the top surface to the bottom surface in the longitudinal direction of the connection block 130. The connection block 130 is coupled to the test case 100 so that the bottom surface thereof faces the exposure chamber C, and the particle injection port 110 at one end of the injection flow path 131 formed on the side of the connection block 130. ) May be coupled and the suction discharge port 120 may be coupled to one end of the discharge passage 132 formed on the top surface of the connection block 130. At this time, the particle injection port 110 may be coupled in a manner that is sealedly inserted into the injection flow path 131, as shown in Figure 2, the suction discharge port 120 is to seal the outer peripheral surface of the upper end of the connection block 130 Can be combined in a wrapping manner. Particle injection port 110 and the suction discharge port 120 may be inserted into a separate sealing material (S) for the mutual sealing coupling portion coupled to the connection block 130.

As described above, the particle injection port 110 and the suction discharge port 120 are coupled to the connection block 130, and the connection block 130 is coupled to the test case 100 to expose the exposure chamber C. The injection port 110 and the suction discharge port 120 may be configured to be in communication with the exposure chamber C. In contrast, in one embodiment of the present invention, a more efficient flow of nanoparticles inside the exposure chamber C may be achieved. The connection block 130 is connected to the test case 100 so that the particle injection port 110 and the suction discharge port 120 communicate with the exposure chamber C through the suction flow hole 101 and the block coupling part 102. Can be combined.

That is, in the test case 100, as shown in FIG. 3, a suction flow hole 101 is formed through the opposite side of the open surface, and a connection block 130 is formed around the outer edge of the suction flow hole 101. A separate block coupling portion 102 is formed to protrude on the outer circumferential surface of the test case 100 so as to be sealed. The connection block 130 is hermetically coupled to the block coupling portion 102 through a separate sealing material (S). When the connection block 130 is coupled to the block coupling unit 102 as described above, the injection passage 131 and the discharge passage 132 formed in the connection block 130 are exposed through the suction flow hole 101. The particle injection port 110 and the suction discharge port 120, which are in communication with C) and coupled to the injection passage 131 and the discharge passage 132, respectively, are also in communication with the exposure chamber C as well.

In this case, a separate particle injection tube 140 is injected into the connection block 130 so that the nanoparticles introduced through the particle injection port 110 pass through the injection passage 131 and then flow into the exposure chamber C. Communicatingly coupled to the lower end of the flow path 131, the particle injection tube 140 is disposed so as to protrude in the exposure chamber (C) at the end toward the open one surface of the test case (100). The particle injection tube 140 is coupled to protrude into the exposure chamber (C) through the suction flow hole (101).

The particle injection tube 140 is for allowing the nanoparticles introduced through the particle injection port 110 to be injected into the exposure chamber C after passing through the injection passage 131 of the connection block 130. It may be formed in a pipe shape, the nanoparticles injected through the particle injection tube 140 flows intensively toward the test object (T) in contact with the open one surface of the test case 100, as shown in FIG. It is preferable that the end of the particle injection tube 140 is disposed to protrude in the exposure chamber (C) so as to be close to the open one surface. At this time, the particle injection tube 140 is preferably coupled to the block coupling portion 102 to be linearly movable to adjust the height of the protrusion (L), a detailed description thereof will be described later.

In addition, the particle injection tube 140 is coupled to the injection flow path 131 of the connection block 130 and the straight pipe portion 141 and the straight pipe portion that is linearly disposed with the same diameter toward the open one surface of the test case 100 ( It may be configured to include an expansion portion 142 is formed extending in one end of the 141, the diameter is expanded closer to the open one surface of the test case 100. According to this structure, the nanoparticles diffuse and flow through the expansion portion 142 of the particle injection tube 140 and concentrate in a relatively uniform distribution toward the test object T disposed on an open surface of the test case 100. do.

Looking at the operating state of the portable nanoparticle exposure apparatus according to an embodiment configured as described above, first, the test object (T) is placed inside the housing 200, the test case to surround the test object (T) In a state where the 100 is disposed inside the housing 200, the inlet 211 of the housing 200 and the particle injection port 110 of the test case 100 are connected to each other through an inflow pipe P. A suction pipe Q connected to the vacuum pump R is connected to the suction discharge port 120 of the test case 100. As described above, the exposure chamber C is sucked through the vacuum pump R connected to the suction discharge port 120 while the test case 100 is fixed in position. When the exposure chamber (C) space is sucked by the vacuum pump (R), a vacuum pressure is formed in the exposure chamber (C), so that the test case 100 is tested on one surface that is open so that the exposure chamber (C) is sealed. The vacuum is compressed to the bottom surface of the housing 200 while wrapping the object T. In addition, when the vacuum pressure is formed in the exposure chamber C as described above, nanoparticles floating outside the housing 200 are compensated by the inlet 211 and the particle injection port 110 in an aerosol state to compensate for the vacuum pressure. Continuously introduced into the exposure chamber (C).

That is, when the exposure chamber (C) space is sucked through the vacuum pump (R) while the test case (100) is fixed in contact with the bottom surface of the housing (200), the test is performed as the vacuum pressure is formed in the exposure chamber (C). While the case 100 is vacuum-compressed to the housing 200, nanoparticles are continuously introduced into the exposure chamber C through the particle injection tube 140. Therefore, since the inner space of the exposure chamber C is sealed to be blocked from the outside by the vacuum pressing of the test case 100, the nanoparticles continuously supplied to the exposure chamber C are stably exposed to the exposure chamber C without external leakage. Supplied to. At this time, it is preferable that a separate rubber packing 150 that can be crimped as shown in FIG. 3 is coupled to one open end of the test case 100, and thus, the vacuum pressing process of the test case 100 is more smoothly performed. May be performed.

According to the structure and the operating principle of the portable nanoparticle exposure apparatus according to an embodiment of the present invention after placing the housing 200 in a place to measure the toxicity evaluation test for nanoparticles, nanoparticles floating in the outside atmosphere When the vacuum pump (R) is kept in operation for a predetermined time so that the gas flows into the test case (100) through the inlet (211), the test object (T) inside the test case (100) in the actual field nano Because of the intensive exposure of particles, toxicological evaluation tests on nanoparticles in the field can be performed more accurately.

In addition, the portable nanoparticle exposure apparatus according to the present invention, as described above, the external leakage from the inside of the test case 100 for the nanoparticles introduced into the test case 100 from the outside of the housing 200 through the inlet 211. Since this does not occur, the flow rate conditions for the nanoparticles exposed to the test object T in the exposure chamber C can be controlled relatively precisely, and thus the effects on the test subject T under various flow rate conditions of the nanoparticles. The impact can be measured more accurately, which improves the reliability of toxicity assessment tests on nanoparticles.

In particular, as described above, since the particle injection tube 140 is disposed to protrude into the exposure chamber C to face an open side of the test case 100, the nanoparticles injected into the exposure chamber C are externally lost. Without exposure to the test subject T more intensively, thereby more accurately measuring the effect of the nanoparticles on the test subject T. That is, the nanoparticles injected into the exposure chamber C through the particle injection tube 140 are opened in the test case 100 through the expansion part 142 of the particle injection tube 140 as shown in FIG. 3. The test object T may be more uniformly concentrated on the test object T exposed on one surface, thereby maintaining a sufficient level of exposure to the test object T. Thereafter, the nanoparticles are partially discharged from the exposure chamber C to the outside through the suction discharge port 120 in contact with or embedded in the surface of the test object T, which is a tissue cell, or on the surface of the test object T. It may also remain stuck.

As described above, the portable nanoparticle exposure apparatus according to the exemplary embodiment of the present invention affects the test object T in such a way that most of the nanoparticles contact or get stuck through the particle injection tube 140. It is configured to extend, and is configured to minimize the nanoparticles introduced into the exposure chamber (C) is discharged directly to the suction discharge port 120 without affecting the test object (T).

FIG. 5 is a schematic cross-sectional view illustrating an internal structure of a nanoparticle exposure apparatus and a protruding height change state of a particle injection tube according to another exemplary embodiment.

As shown in FIG. 5, the connection block 130 to which the particle injection tube 140 is coupled may have a block coupling portion 102 so that the protrusion height of the particle injection tube 140 protrudingly disposed in the exposure chamber C may be adjusted. ) Is coupled to move up and down linearly. That is, the connection block 130 is coupled to the block coupling portion 102 so as to be movable up and down linearly, so that the protrusion height of the particle injection tube 140 coupled to the connection block 130 with respect to the exposure chamber C is integrally formed. It can be configured to be adjusted. Of course, in this case, it is also possible to simply adjust the coupling state of the connection block 130 and the particle injection tube 140 to adjust the height of the protrusion of the particle injection tube 140, the connection block 130 is a test case ( It will be easier to adjust the protruding height of the particle injection tube 140 in a manner to adjust the coupling state of the connection block 130 and the test case 100 without separating from the 100.

To this end, an uneven portion 103 may be formed on an inner surface of the block coupling portion 102, and a guide protrusion 133 may be formed on an outer circumferential surface of the bottom of the connection block 130 to be engaged with the uneven portion 103. . Therefore, the connection block 130 may be configured such that the guide protrusion 133 is engaged with the concave-convex portion 103 and can be moved up and down step by step, so that the protrusion height of the particle injection tube 140 is shown in FIG. 5. As well as for example L1 and L2. The protruding height adjustment configuration of the particle injection tube 140 can be changed in various ways. For example, threads corresponding to each other are formed on the outer circumferential surface of the connection block 130 and the inner circumferential surface of the block coupling portion 102 to each other. It may also be configured in such a way as to be screwed.

As such, when the protrusion height of the particle injection tube 140 is adjusted, the flow of nanoparticles exposed to the test object T may be changed, that is, the protrusion height of the particle injection tube 140 is increased to increase the test object ( As it is closer to T), the flow of nanoparticles can be exposed to the test subject T in a more intense state, and thus, a toxicity evaluation test for nanoparticles can be performed under more various conditions.

On the other hand, although the particle injection tube 140 may be formed in the form of a pipe in which both ends of the inlet and the outlet are completely open as shown in FIG. 2, a plurality of particle flow holes in one end is closed as shown in FIG. 5. 143 may be formed in a formed manner. That is, the inlet of the particle injection tube 140 coupled to the injection flow path 131 of the connection block 130 is formed in a fully open shape, and faces the open one surface of the test case 100 to the exposure chamber C. The outlet of the particle injection tube 140 exposed may be configured in a form in which a plurality of particle flow holes 143 are formed in a closed state.

According to this structure, the flow of nanoparticles flowing toward the open one surface of the test case 100 through the particle injection tube 140 may be diffused to a wider area through the plurality of particle flow holes 143. Accordingly, the nanoparticles may be more uniformly exposed to a wider area of the test object T disposed on the open side of the test case 100, thereby enabling a more accurate toxicity evaluation test for the nanoparticles.

4 and 5 are diagrams for experimentally analyzing the flow of nanoparticles injected into the exposure chamber of the portable nanoparticle exposure apparatus according to an embodiment of the present invention.

4 illustrates a state in which the nanoparticle flow in the aerosol state is plotted in the state where the inner diameter of the straight tube portion 141 of the particle injection tube 140 is 4 mm, and FIG. 5 illustrates the straight tube portion of the particle injection tube 140. (141) Shown is a state depicted by analyzing the flow of nanoparticles in an aerosol state with an internal diameter of 2 mm.

As shown in FIGS. 4 and 5, the flow of the nanoparticles is further diffused from the straight pipe portion 141 of the particle injection tube 140 through the expansion tube 142 and concentrated on an open side of the test case 100. It shows an aspect. As shown in FIG. 5, it can be seen that the flow rate of the nanoparticles increases in the state in which the inner diameter of the straight pipe portion 141 is smaller to 2 mm, so that the concentration of the nanoparticles is more concentrated on an open surface of the test case 100. In particular, the nanoparticles injected from the particle injection tube 140 into the exposure chamber C are distributed evenly over the entire area of the open side of the test case 100 by the expansion unit 142, and the open side At the edges of the vortex flow is formed and the nanoparticles are more concentrated on the open side again.

As such, the flow of nanoparticles injected into the exposure chamber C through the particle injection tube 140 is concentrated on one open surface of the test case 100, and thus is in contact with the open surface of the test case 100. Because of the focus on the test subject T, toxicity assessment tests for more accurate nanoparticles can be performed in vitro, as described above.

FIG. 6 is a cross-sectional view schematically illustrating a state in which the internal structure and the protrusion height of the particle injection tube of the portable nanoparticle exposure apparatus according to another embodiment of the present invention are changed.

As shown in FIG. 6, the connection block 130 to which the particle injection tube 140 is coupled may have a block coupling portion 102 so that the protrusion height of the particle injection tube 140 protrudingly disposed in the exposure chamber C may be adjusted. ) Can be coupled to move up and down linearly. That is, the connection block 130 is coupled to the block coupling portion 102 so as to be movable up and down linearly, so that the protrusion height of the particle injection tube 140 coupled to the connection block 130 with respect to the exposure chamber C is integrally formed. It can be configured to be adjusted. Of course, in this case, it is also possible to simply adjust the coupling state of the connection block 130 and the particle injection tube 140 to adjust the height of the protrusion of the particle injection tube 140, the connection block 130 is a test case ( It will be easier to adjust the protruding height of the particle injection tube 140 in a manner to adjust the coupling state of the connection block 130 and the test case 100 without separating from the 100.

To this end, an uneven portion 103 may be formed on an inner surface of the block coupling portion 102, and a guide protrusion 133 may be formed on an outer circumferential surface of the bottom of the connection block 130 to be engaged with the uneven portion 103. . Therefore, the connection block 130 may be configured such that the guide protrusion 133 is engaged with the concave-convex portion 103 and can be moved up and down step by step, so that the protruding height of the particle injection tube 140 is as shown in FIG. As well as for example L1 and L2. The protruding height adjustment configuration of the particle injection tube 140 can be changed in various ways. For example, threads corresponding to each other are formed on the outer circumferential surface of the connection block 130 and the inner circumferential surface of the block coupling portion 102 to each other. It may also be configured in such a way as to be screwed.

On the other hand, although the particle injection tube 140 may be formed in the form of a pipe in which both ends of the inlet and the outlet are completely open as shown in FIG. 3, a plurality of particle flow holes in one end is closed as shown in FIG. 6. 143 may be formed in a formed manner. That is, the inlet of the particle injection tube 140 coupled to the injection flow path 131 of the connection block 130 is formed in a fully open shape, and faces the open one surface of the test case 100 to the exposure chamber C. The outlet of the particle injection tube 140 exposed may be configured in a form in which a plurality of particle flow holes 143 are formed in a closed state.

According to this structure, the flow of nanoparticles flowing toward the open one surface of the test case 100 through the particle injection tube 140 may be diffused to a wider area through the plurality of particle flow holes 143. Accordingly, the nanoparticles may be more uniformly exposed to a wider area of the test object T disposed on the open side of the test case 100, thereby enabling a more accurate test for toxicity evaluation of the nanoparticles.

FIG. 7 is a partially exploded perspective view schematically illustrating a configuration of a portable nanoparticle exposure apparatus according to another embodiment of the present invention, and FIG. 8 schematically illustrates an internal structure of the portable nanoparticle exposure apparatus illustrated in FIG. 7. One cross section.

The portable nanoparticle exposure apparatus according to another embodiment of the present invention may be configured such that a plurality of test cases 100 are disposed in one housing 200, as shown in FIGS. 7 and 8, respectively. Particle injection port 110 respectively coupled to the test case 100 of the may be configured to be connected to one inlet 211 through each branched inlet pipe (P). That is, one end of the inflow pipe (P) is formed to be connected to one inlet 211 formed in the housing 200, the other end is to be connected to each of the particle injection port 110 coupled to each test case 100 It is formed in a branched form. In addition, the suction discharge port 120 coupled to the plurality of test cases 100 is also configured to be connected to one vacuum pump R through the suction pipe Q of the branched form in the same manner.

As described above, a plurality of test cases 100 are disposed in one housing 200, and nanoparticles are introduced into each test case 100 from the same one inlet 211, and at the same time, one vacuum pump R By forming the same vacuum pressure in the exposure chamber (C) through), nanoparticles are exposed to the test object (T) under the same conditions in the exposure chamber (C) of each test case 100. Therefore, by measuring the characteristic change of the test object (T) exposed to the nanoparticles under the same conditions in each test case 100, it is possible to complementally evaluate each measurement result through each measurement result, one measurement The effect of performing multiple measurements under the same conditions can be achieved.

Of course, in this case, the suction discharge ports 120 coupled to the plurality of test cases 100 are each connected to separate vacuum pumps R through independent suction pipes Q, and each vacuum pump R By forming different vacuum pressures in each of the exposure chambers C, the nanoparticles may be exposed to the test object T under different conditions for each test case 100. In this case, it is possible to obtain the effect of performing multiple measurements under different conditions through one measurement.

The above description is merely illustrative of the technical idea of the present invention, and those skilled in the art to which the present invention pertains may make various modifications and changes without departing from the essential characteristics of the present invention. Therefore, the embodiments disclosed in the present invention are intended to illustrate rather than limit the scope of the present invention, and the scope of the technical idea of the present invention is not limited by these embodiments. The scope of protection of the present invention should be construed according to the following claims, and all technical ideas falling within the scope of the same shall be construed as falling within the scope of the present invention.

100: test case 110: particle injection port
120: suction discharge port 130: connection block
140: particle injection tube 150: rubber packing
200: housing

Claims

A test object seated therein and a housing in which one side of the inlet is formed; And
An exposed chamber is formed inside the one surface in an open form and disposed inside the housing to surround the test object, and on one side, a particle injection port connected through the inlet and the inlet pipe, and a separate vacuum pump and a suction pipe. A test case in which a connected suction discharge port is connected in communication with the exposure chamber
And a vacuum pressure is formed in the exposure chamber through the vacuum pump, and the test case surrounds the test object and is vacuum-compressed to the inner side of the housing to seal the exposure chamber, and at the same time, the particles Portable nanoparticle exposure device, characterized in that the nanoparticles are suspended in the floating through the injection port continuously outside.

The method of claim 1,
Portable nanoparticle exposure apparatus characterized in that a separate rubber packing is coupled to the open one end of the test case.

The method of claim 2,
One side of the test case is connected to the connection block formed in each of the injection passage and the discharge passage communicating with the exposure chamber independently therein, the particle injection port is in communication with one end of the injection passage and the suction discharge port Is coupled in communication with one end of the discharge flow path, and the other end of the injection flow path is characterized in that a separate particle injection tube is communicatively coupled to protrude into the exposure chamber so that the nanoparticles can flow into the exposure chamber. Exposure device.

The method of claim 3, wherein
The particle injection tube
A straight pipe portion coupled to an injection channel of the connection block and straightly disposed at the same diameter toward an open surface of the test case; And
An extension portion formed at one end of the straight pipe portion, the diameter of which extends closer to an open surface of the test case.
Portable nanoparticle exposure device comprising a.

The method according to any one of claims 1 to 4,
And a plurality of test cases are disposed in the housing, and the particle injection ports coupled to each test case are connected to the inlets of the housing through branched inlet pipes, respectively.

The method of claim 5, wherein
The portable nanoparticle exposure apparatus of claim 1, wherein the suction discharge ports coupled to the plurality of test cases are connected to one vacuum pump through branched suction pipes, respectively.

The method of claim 5, wherein
The portable nanoparticle exposure apparatus of claim 1, wherein the suction discharge ports coupled to the plurality of test cases are connected to separate vacuum pumps through independent suction pipes, respectively.