KR101204644B1 - nitrate concentration detecting agent and the kit using thereof - Google Patents
nitrate concentration detecting agent and the kit using thereof Download PDFInfo
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- KR101204644B1 KR101204644B1 KR1020110069815A KR20110069815A KR101204644B1 KR 101204644 B1 KR101204644 B1 KR 101204644B1 KR 1020110069815 A KR1020110069815 A KR 1020110069815A KR 20110069815 A KR20110069815 A KR 20110069815A KR 101204644 B1 KR101204644 B1 KR 101204644B1
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Abstract
Description
본 발명은 수질측정 방법 중 질산성 질소 농도 검출방법 중 흡광광도법 또는 비색표대조법에 사용하는 질산성 질소 농도 검출시약에 관한 것으로, 포스포릭산 용액(Phosphoric Acid Solution) 및 디메틸페놀 용액(2,6-dimethylphenol Solution)으로 구성된 질산성질소 농도 검출시약을 시료에 혼합하여 나타나는 색상을 흡광광도법 또는 비색표대조법 등을 이용하여 질산성질소 농도를 간단하고 신속하게 측정하게 하는 검출 시약 및 이를 포함하는 검출키트에 관한 것이다.
The present invention relates to a nitrate nitrogen concentration detection reagent used in the absorbance or colorimetric control method of the nitrate nitrogen concentration detection method of water quality, phosphoic acid solution (Phosphoric Acid Solution) and dimethylphenol solution (2, 6 a detection reagent and a detection kit including the same, in which a nitrate concentration detection reagent composed of -dimethylphenol solution is mixed with a sample to measure the nitrate concentration simply and quickly by using an absorbance method or a colorimetric control method. It is about.
최근 들어 하천수, 호수, 폐수 중에 포함된 질소 또는 인 등의 오염원에 대한 환경규제가 점점 강화됨에 따라 방류수 또는 하천수에 대하여 여러 형태의 수질측정이 행하여 지고 있다. 이러한 수질측정 시 요구되는 특성으로는 정밀성과 재현성을 보장해야 하는 기본적인 요구사항 이외에 신속하고 편리해야 하며, 환경친화적이어야 하고 경제성을 갖추어야 한다.Recently, as environmental regulations on pollutants such as nitrogen and phosphorus contained in river water, lakes and waste water are gradually strengthened, various types of water quality measurements have been made on discharge water or river water. In addition to the basic requirements to ensure precision and reproducibility, the characteristics required for water quality measurement should be fast, convenient, environmentally friendly and economical.
특히, 질산성질소(nitrate, NO3-)는 널리 알려진 수질오염 물질의 하나이다. 질산염 이온에 의한 광범위한 오염은 환경 및 인체건강에 대하여 심각한 위협이 되고 있다. 따라서 질산염 이온의 분석은 매우 중요하다. 장기간 높은 농도의 질산염을 섭취하면 소화계통의 암을 유발할 수 있는 가능성이 매우 높다. 또한 높은 농도의 질산성질소에 노출되는 경우 청색아증(Blue Baby)의 원인이 되곤 한다.In particular, nitrate (NO 3- ) is one of the well-known water pollutants. Extensive contamination with nitrate ions poses a serious threat to the environment and human health. Therefore, the analysis of nitrate ions is very important. Ingestion of high levels of nitrate for a long time is very likely to cause cancer of the digestive system. In addition, exposure to high concentrations of nitric acid is a cause of blue baby (Blue Baby).
대한민국 수질오염공정시험법에서는 대표적인 흡광광도법으로 부루신법과 자외선흡광광도법을 사용하고 있다.Korea's water pollution process test method uses the leucine method and ultraviolet absorbance method as representative absorbance method.
상기한 부루신법은 살리실산나트륨, 설파민산암모늄, 염화나트륨 등의 시약을 이용하고 수욕상에서 증발 건고하는 복잡한 절차를 요하며 시간이 오래 걸리는 문제점이 있다.The above-mentioned leucine method requires a complicated procedure of using a reagent such as sodium salicylate, ammonium sulfamate, sodium chloride, and evaporating to dryness in a water bath and takes a long time.
또한 이런 방법들은 특이성이 떨어져서 많은 이온들이 방해작용을 한다. 따라서 측정하기 전에 전처리 과정이 필요하여 불편하고 시간이 많이 요구되는 단점이 있다.Also, these methods are less specific, causing many ions to interfere. Therefore, it is inconvenient and time-consuming because it requires a pretreatment process before measurement.
이와 같은 이유로 특허출원 10-2005-0043195는 질산염 이온 검출용 바이오센서 및 이를 이용한 측정방법에 관한 것으로서, 더욱 구체적으로는 질산염 환원효소(NaR)를 표면에 갖는 작동 전극과 기준 전극 및 상대 전극을 갖는 바이오센서 및 이를 이용하여 시료 중의 질산염 농도를 측정하는 방법 또는 질산염이 아닌 질소 화합물을 질산염으로 전환시킴으로써 총질소의 농도를 측정할 수 있게 하는 질산염 이온화 반응기에 관한 것을 제시한 바 있다.For this reason, patent application 10-2005-0043195 relates to a biosensor for detecting nitrate ions and a measuring method using the same. More specifically, the patent application 10-2005-0043195 has a working electrode having a nitrate reductase (NaR), a reference electrode, and a counter electrode. Biosensors and methods for measuring the nitrate concentration in a sample using the same or a nitrate ionization reactor for converting nitrogen compounds other than nitrates into nitrates can be measured.
또한, 공개특허 10-2005-0111069는 질산성 질소의 분석은 분말시약인 환원제, 액체시약인 발색보조제, 액체시약인 발색제로 구성됨을 특징으로 하여, 시료에 산화보조제로 가성소다와 붕소산의 혼합물, 산화제로 과황산칼륨을 첨가하여 열반응시켜 유기성 질소 및 암모니아성 질소를 질산성 질소로 변환시키고, 여기에 환원제를 첨가하여 질산성 질소를 아질산성 질소로 변환시키며, 여기에 다시 발색보조제로 슬퍼닐아미드, 발색제로 염화에틸렌디아민을 첨가하여 발색시킨 후 분광광도계로 흡광도를 측정하여 총질소를 정량하는 방법을 사용하면서, 총질소의 농도가 10ppm까지인 시료 1밀리리터에 대해 무게비로 산화보조제로 가성소다를 0.002내지 0.0012, 붕소산을 0.009내지 0.006, 산화제로 과황산칼륨을 0.009 내지 0.006, 발색보조제로 슬퍼닐아미드를 0.0001내지 0.0005, 발색제로 염화에틸렌디아민을 0.0000198내지 0.000025의 비율로 첨가한 것을 특징으로 하는 분석시약 조성물 및 이를 이용하는 분석방법을 제공한 바 있다.
In addition, the patent 10-2005-0111069 is characterized in that the analysis of nitrate nitrogen consists of a powder reagent reducing agent, a liquid reagent coloring aid, a liquid reagent coloring agent, a mixture of caustic soda and boric acid as an oxidizing aid to the sample. , By adding potassium persulfate as an oxidizing agent and thermally reacting to convert organic nitrogen and ammonia nitrogen to nitrate nitrogen, and adding a reducing agent to convert nitrate nitrogen to nitrite nitrogen, which is sad again as a color aid Nylamide and ethylenediamine chloride were added as a coloring agent, followed by color spectrophotometry to measure the absorbance, and quantitative determination of total nitrogen. 0.002 to 0.0012 soda, 0.009 to 0.006 boric acid, 0.009 to 0.006 potassium persulfate as an oxidizing agent, and 0.0001 to 0.0001 to sulfanylamide as a color aid 0.0005, and a service 0.0000198 chloride ethylenediamine as developers to analysis reagents, characterized in that added at the rate of 0.000025 compositions and assay method using the same bar.
상기한 선행기술들은 종래의 질산성 질소 분석방법에 비하여 진보된 기능과 효과가 있는 발명이라는 면은 있다.The prior art is an invention that has an advanced function and effect as compared to the conventional nitrate nitrogen analysis method.
하지만, 전자의 선행기술은 전기화학적인 바이오센서의 구성적인 복잡함과 질산염환원효소가 고정된 탄소전극 등의 관리의 불편함을 초래하여 실용성을 확보할 수 없는 단점을 가지고 있었다.However, the former prior art had a disadvantage in that it is not possible to secure practicality because of the constitutive complexity of the electrochemical biosensor and the inconvenience of management of the carbon electrode to which the nitrate reductase is fixed.
또한 후자의 선행기술은 질산성질소를 아질산성질소로 환원시키는 과정과 발색보조제와 발색제를 첨가하여 발색하는 과정을 별도로 처리함으로써 시간이 많이 들고 번거로운 면이 있으며, 산화보조제인 가성소다, 붕소산, 산화제인 과황산칼륨 등은 종래의 산화제의 기능과 효과가 유사하였고, 발색제인 염화나프틸에틸디아민 및 발색보조제인 슬퍼닐아미드는 발색면에서 효과가 떨어져서 정확도가 떨어지는 단점이 있었다.In addition, the latter prior art is a time-consuming and cumbersome process by reducing the process of reducing nitrate nitrogen to nitrite nitrogen and the process of coloring by adding a color aid and a coloring agent, caustic soda, boric acid, oxidizing agent Phosphorous potassium persulfate and the like were similar in function and effect of the conventional oxidizing agent, naphthylethyldiamine chloride and the color supplements sulfanylamide has a disadvantage in that the accuracy is poor in terms of color development.
따라서, 상기한 단점을 개선하여 절차가 간단하고 시간을 단축시키며 정확한 측정을 필요로 하는 질산성질소 농도 검출시약 및 검출키트가 절실히 요구되고 있다.
Therefore, there is an urgent need for a nitrogen nitrate concentration detection reagent and a detection kit that remedy the shortcomings, thus simplifying the procedure, shortening the time, and requiring accurate measurement.
본 발명은 상기와 같은 문제점을 해결하기 위하여,In order to solve the above problems,
질산성질소 농도를 측정하는 시약에 있어서, 포스포릭산 용액 또는 디메틸페놀 용액 중 하나 이상으로 구성한 질산성질소 농도 검출시약 및 검출키트를 제공한다.In the reagent for measuring the nitric acid concentration, a reagent and a detection kit for detecting the nitric acid concentration comprising at least one of a phosphoric acid solution or a dimethylphenol solution are provided.
또한 상기 포스포릭산 용액은 증류수, Phosphoric Acid, Sulfuric Acid를 혼합하여 조성하고, 상기 디메틸페놀 용액은 증류수, dimethylphenol, Isopropanol을 혼합하여 조성한 것을 특징으로 하는 질산성질소 농도 검출시약 및 검출키트를 제공한다. In addition, the phosphoric acid solution is prepared by mixing distilled water, Phosphoric Acid, Sulfuric Acid, and the dimethylphenol solution is a mixture of distilled water, dimethylphenol, Isopropanol provides a nitrogen nitrate concentration detection reagent and detection kit. .
또한 포스포릭산 용액은 증류수 100중량부에, Phosphoric Acid 200~400중량부, Sulfuric Acid400~800중량부를 혼합하여 조성하고, 디메틸페놀 용액은 증류수 100중량부에, dimethylphenol 0.5~4중량부, Isopropanol 15~35중량부 혼합하여 조성한 것을 특징으로 하는 질산성질소 농도 검출시약 및 검출키트를 제공한다.
In addition, the phosphoric acid solution is mixed with 100 parts by weight of distilled water, 200 to 400 parts by weight of Phosphoric Acid, 400 to 800 parts by weight of sulfuric acid, and the dimethylphenol solution is contained in 100 parts by weight of distilled water, 0.5 to 4 parts by weight of dimethylphenol, and Isopropanol 15 Provided is a nitric acid nitrate concentration detection reagent and detection kit, characterized in that by mixing ~ 35 parts by weight.
본 발명의 질산성질소 농도 검출시약을 통하여 질산성 질소 농도를 측정하는 경우 통상의 질산성질소 농도 검출 방법에 비하여 현저하게 시간을 단축하여 측정할 수 있는 효과가 있다.In the case of measuring the nitrate nitrogen concentration through the nitrate nitrogen concentration detection reagent of the present invention, there is an effect that can be measured by a significantly shorter time than the conventional nitrate concentration detection method.
또한, 본 발명은 통상의 질산성질소 농도 검출방법에 비하여 측정절차가 매우 간단하여 많은 수고를 덜어주는 효과가 있다.In addition, the present invention has a very simple measurement procedure compared to the conventional nitrogen nitrate concentration detection method has the effect of reducing a lot of effort.
그리고, 본 발명은 질산성질소 농도 측정 방법에서 나타나는 시료 중에 포함된 다른 종류의 이온에 의한 간섭(Interference)의 영향을 배제할 수 있는 현저한 효과가 있다. 특히 질산성질소 측정시 가장 큰 간섭요소인 불소의 간섭을 배제하는데 현저한 효과가 있다.In addition, the present invention has a remarkable effect of eliminating the influence of interference by other kinds of ions contained in the sample appearing in the method for measuring the nitrate nitrogen concentration. In particular, it has a remarkable effect in excluding the interference of fluorine, which is the biggest interference factor when measuring nitrate nitrogen.
이뿐만 아니라 측정시료의 pH에 관계없이 측정할 수 있어 측정시료의 pH 조절을 할 필요성이 없는 효과가 있다.In addition, it can be measured irrespective of the pH of the measurement sample, there is no need to adjust the pH of the measurement sample.
이와 더불어, 본 발명의 질산성 질소 농도 검출시약은 제조가 용이하고 저렴하다는 강점이 있다.In addition, the nitrate nitrogen concentration detection reagent of the present invention has the advantage of easy manufacturing and low cost.
또한 종래의 질산성 질소 농도 검출 방법은 시료를 증발 건고하고 황산을 가하며 다시 가열하면서 수산화 나트륨 등을 가하는 등의 복잡한 절차가 필요하나 본 발며에 따른 질산성 질소 농도 검출시약을 사용하는 경우 이러한 불편한 문제점을 해결하는 효과가 있다.
In addition, the conventional method for detecting the concentration of nitrate nitrogen requires a complicated procedure such as evaporating and drying the sample, adding sulfuric acid, and heating again, adding sodium hydroxide, etc., but it is inconvenient to use the nitrate concentration detection reagent according to the present invention. Has the effect of solving.
이하 본 발명을 상세히 설명한다.
Hereinafter, the present invention will be described in detail.
본 발명은 질산성질소(NO3-N, Nitrate)의 농도를 검출하기 위한 방법인 흡광광도법 또는 비색표대조법을 이용하기 위하여, 시료 중의 질산성질소와 반응하여 를 발색하는 물질인 질산성질소 농도 검출시약을 제공한다.In the present invention, in order to use the absorbance method or the colorimetric control method, which is a method for detecting the concentration of nitrogen nitrate (NO 3 -N, Nitrate), the concentration of nitrogen nitrate, which is a substance that reacts with nitrogen nitrate in a sample and develops. Provide a detection reagent.
또한, 수질을 측정하기 위한 검출키트로서 상기한 질산성질소 농도 검출시약을 포함한 질산성질소 농도 검출키트를 제공한다. In addition, there is provided a nitrate nitrogen concentration detection kit including the above nitrate concentration detection reagent as a detection kit for measuring the water quality.
본 발명에서 검출키트라 함은 수질을 측정 또는 분석하기 위한 장비 또는 장치로서 검출시약을 사용하여 수질을 분석하는 장치 또는 장비를 의미하며, 보다 구체적으로는 상기한 흡광광도법, 비색표대조법 등과 같이 시료의 색의 변화 등과 같은 물리적 화학적 변화를 통하여 수질을 분석할 수 있는 장치 또는 장비를 의미한다.
In the present invention, the detection key means an apparatus or equipment for analyzing water quality using a detection reagent as an equipment or apparatus for measuring or analyzing water quality, and more specifically, a sample such as the absorbance spectroscopy and colorimetric control method. Refers to a device or equipment that can analyze water quality through physical and chemical changes such as color change.
본 발명의 질산성질소 농도 검출시약은 포스포릭산 용액(Phosphoric Acid Solution) 또는 디메틸페놀 용액(dimethylphenol Solution) 중 어느 하나 이상으로 구성된다.The reagent for detecting the nitrate nitrogen concentration of the present invention is composed of at least one of a phosphoric acid solution (Phosphoric Acid Solution) or a dimethylphenol solution (dimethylphenol Solution).
따라서 본 발명의 질산성질소 농도 검출시약은 포스포릭산 용액(Phosphoric Acid Solution)으로 구성될 수 있다.Accordingly, the reagent for detecting the nitrate concentration of nitrate of the present invention may be composed of a phosphoric acid solution.
또한 본 발명의 질산성질소 농도 검출시약은 디메틸페놀 용액(dimethylphenol Solution)으로 구성될 수 있다.In addition, the nitrogen nitrate concentration detection reagent of the present invention may be composed of a dimethylphenol solution (dimethylphenol Solution).
또한 본 발명의 질산성질소 농도 검출시약은 포스포릭산 용액(Phosphoric Acid Solution) 및 디메틸페놀 용액(dimethylphenol Solution)으로 구성될 수 있다.
In addition, the nitrogen nitrate concentration detection reagent of the present invention may be composed of a phosphonic acid solution (Phosphoric Acid Solution) and dimethylphenol solution (dimethylphenol Solution).
상기한 포스포릭산 용액은 증류수, Phosphoric Acid, Sulfuric Acid를 혼합하여 조성한다.The phosphoric acid solution is prepared by mixing distilled water, Phosphoric Acid, Sulfuric Acid.
바람직하게는 증류수 100중량부에 Phosphoric Acid 200~400중량부, Sulfuric Acid 400~800중량부를 혼합하여 조성하는 것이 좋다. 이와 같은 구성으로 조성한 경우 시료와 반응하여 발색하는 작용을 상승시키는 효과를 나타낸다.
Preferably, 100 parts by weight of distilled water may be mixed with 200 to 400 parts by weight of Phosphoric Acid and 400 to 800 parts by weight of sulfuric acid. In the case of such a composition, the effect of increasing the color reaction by reacting with the sample is obtained.
Phosphoric Acid는 인산을 의미하며 화학식은 H3PO4, 오르소인산, 정인산이라고도 한다. 무색투명한 시럽상 무취의 액체로, 공기 중의 습기를 흡수하기 쉽고 유기물에 접촉하면 착색한다. 무수물은 주상 결정. 보통 인산은 물을 22% 정도 가지고 있다. 본 발명에서 인산은 바람직하게는 CAS No.: 7664-38-2를 사용하는 것이 좋다.
Phosphoric Acid means phosphoric acid, and the chemical formula is also called H3PO4, orthophosphoric acid, and regular phosphoric acid. Colorless, transparent syrupy, odorless liquid, easy to absorb moisture in the air and colored when in contact with organic matter. Anhydrides are columnar crystals. Usually phosphoric acid has about 22% water. In the present invention, it is preferable to use phosphoric acid as CAS No .: 7664-38-2.
본 발명에서 일관되게 Cas No.를 기재하여 그 화합물의 특징을 지우는 것은 그 Cas No.의 화합물과 성상이나 물리 화학적 특성이 동일 또는 유사한 성질을 갖는 화합물을 의미하는 것으로 해석하여야 한다.
In the present invention, consistently describing Cas No. and characterizing the compound should be interpreted as meaning a compound having the same or similar properties and physicochemical properties as the Cas No. compound.
Sulfuric Acid는 황산을 의미하며 H2SO4의 화학식을 갖는 무색의 비휘발성 액체로, 공업적으로 백금이나 오산화바나듐 촉매를 이용해 만든다. 물을 제외하고 가장 많이 제조되는 강산성의 화합물이다.Sulfuric acid refers to sulfuric acid and is a colorless nonvolatile liquid having the chemical formula H2SO4. It is industrially produced using platinum or vanadium pentoxide catalyst. Except for water, it is the most strongly produced acidic compound.
본 발명에서 황산은 CAS No.: 7664-93-9를 사용하는 것이 바람직하다.
Sulfuric acid in the present invention Preference is given to using CAS No .: 7664-93-9.
상기한 디메틸페놀 용액은 증류수, dimethylphenol, Isopropanol을 혼합하여 조성한다.The dimethylphenol solution is prepared by mixing distilled water, dimethylphenol, Isopropanol.
바람직하게는 디메틸페놀 용액(dimethylphenol Solution)은 Demineralized Water 100중량부에, dimethylphenol 0.5~4중량부, Isopropanol 15~35중량부 혼합하여 조성한 것을 특징으로 한다.
Preferably dimethylphenol solution (dimethylphenol Solution) is characterized in that the composition was prepared by mixing 100 parts by weight of Demineralized Water, 0.5 to 4 parts by weight of dimethylphenol, 15 to 35 parts by weight of Isopropanol.
본 발명의 dimethylphenol은 통상의 dimethylphenol을 사용해도 좋으며 더욱 바람직하게는 2,6-dimethylphenol을 사용하는 것이 발색 감도가 높아지는 효과가 있다. 더욱 바람직하게는 CAS No.: 576-26-1를 사용하는 것이 좋다.
The dimethylphenol of the present invention may use a conventional dimethylphenol, more preferably using 2,6-dimethylphenol has the effect of increasing the color sensitivity. More preferably, CAS No .: 576-26-1 is preferably used.
본 발명의 Isopropanol은 CH3CH(OH)CH3, 분자량 60.10, 끓는점 82.5℃. 물, 알코올, 에테르, 클로로포름과 잘 혼합되는 가연성 액체이다. 단, 염용액에는 녹지 않으며 동결 방지제, 여러가지 용매, 알칼로이드의 추출이나 화장수 성분으로 사용할 뿐만 아니라 방부제, 살균제 등 의약용으로도 사용한다.Isopropanol of the present invention is CH3CH (OH) CH3, molecular weight 60.10, boiling point 82.5 ℃. It is a flammable liquid that mixes well with water, alcohol, ether and chloroform. However, it does not dissolve in salt solution and is used for extracting antifreeze, various solvents, alkaloids or cosmetics as well as medicaments such as preservatives and fungicides.
본 발명의 Isopropanol은 물에 잘 혼합되는 성질이 있어 상기한 dimethylphenol과 상호 작용을 통하여 시료의 질산성 질소와 잘 반응을 유도해주는 작용을 하게 된다.
Isopropanol of the present invention has a property of being well mixed with water, thereby inducing a reaction with the nitrate nitrogen of the sample through interaction with the dimethylphenol.
본 발명인 포스포릭산 용액과 디메틸페놀 용액으로 구성된 질산성 질소 농도 검출시약을 질산성질소(NO3-N, Nitrate)가 포함된 일정양의 시료에 혼합하여 반응을 유도한다. 이때 포스포릭산 용액과 디메틸페놀 용액을 동시에 또는 순차적으로 일정량을 첨가한 후 일정의 반응시간을 갖는다. 바람직하게는 순차적으로 첨가하는 것이 반응성을 높이게 된다.
Nitrate nitrogen concentration detection reagent consisting of a phosphoric acid solution and a dimethylphenol solution of the present invention is mixed with a predetermined amount of sample containing nitrogen nitrate (NO 3 -N, Nitrate) to induce a reaction. At this time, the phosphoric acid solution and the dimethylphenol solution are added at the same time or sequentially to have a constant reaction time. Preferably, the sequential addition increases the reactivity.
따라서 상기한 반응시간 동안 포스포릭산 용액(Phosphoric Acid Solution)과 디메틸페놀 용액(dimethylphenol Solution)과 만난 질산성질소(NO3-N, Nitrate)는 상호작용을 통해 유색의 4-nitro-2,6-dimethylphenol의 결합물질을 생성하게 되는데, 이 색의 변화 정도를 이용하여 질산성질소(NO3-N, Nitrate)의 농도를 산출할 수 있게 된다.
Therefore, phosphate acid solution (Phosphoric acid solution) and dimethylphenol solution (dimethyl nitrate) (NO 3 -N, Nitrate) that meets during the reaction time is colored 4-nitro-2,6 It produces a binding compound of -dimethylphenol, and the concentration of this color can be used to calculate the concentration of nitric acid (NO 3 -N, Nitrate).
반응시간 동안 포스포릭산 용액, 디메틸페놀 용액과 만난 질산성질소(NO3-N, Nitrate)는 상호작용을 통해 유색의 4-nitro-2,6-dimethylphenol의 결합물질을 생성하게 되는데, 이 색의 변화 정도를 이용하여 질산성질소(NO3-N, Nitrate)의 농도를 산출한다.Phosphoric acid solution, dimethylphenol solution and nitrogen nitrate (NO 3 -N, Nitrate), which interacted with each other during the reaction time, produced a colored 4-nitro-2,6-dimethylphenol binding agent through interaction. The concentration of nitric acid (NO 3 -N, Nitrate) is calculated using the degree of change.
즉, 상기한 시료의 발색한 정도를 흡광광도법 또는 비색표 대조법 등을 통하여 시료의 질산성 질소의 농도를 검출하게 된다.
That is, the concentration of the nitrate nitrogen of the sample is detected by the absorbance method or the colorimetric comparison method.
다음은 본 발명에 따른 실시예를 설명한다. 본 발명의 실시예는 본 발명의 기술적 내용을 제한하는 것이 아니며 권리범위를 축소하여 해석되어 져서는 아니 된다.
The following describes an embodiment according to the present invention. Embodiment of the present invention is not intended to limit the technical content of the present invention and should not be construed to reduce the scope of rights.
<실시예><Examples>
1. 포스포릭산 용액(Phosphoric Acid Solution) 제조
1. Preparation of Phosphoric Acid Solution
<시약1> <Reagent 1>
Synonyms : Demineralized Water(증류수) Synonyms : Demineralized Water
CAS No .: 7732-18-5 CAS No .: 7732-18-5
<시약2><Reagent 2>
Synonyms : Phosphoric Acid Synonyms : Phosphoric Acid
CAS No .: 7664-38-2 CAS No .: 7664-38-2
<시약3><Reagent 3>
Synonyms : Sulfuric Acid Synonyms : Sulfuric Acid
CAS No .: 7664-93-9
CAS No .: 7664-93-9
상기의 시약을 혼합하여 포스포릭산 용액(Phosphoric Acid Solution)의 제조하였으며 시약 별 조합은 다음과 같다.
The reagents were mixed to prepare a Phosphoric Acid Solution, and the combination of reagents was as follows.
2. 디메틸페놀 용액 제조2. Preparation of Dimethylphenol Solution
<시약1> <Reagent 1>
Synonyms : Demineralized Water(증류수) Synonyms : Demineralized Water
CAS No .: 7732-18-5 CAS No .: 7732-18-5
<시약2><Reagent 2>
Synonyms : 2,6-dimethylphenol Synonyms : 2,6-dimethylphenol
CAS No .: 576-26-1 CAS No .: 576-26-1
<시약3><Reagent 3>
Synonyms : Isopropanol Synonyms : Isopropanol
CAS No .: 67-63-0
CAS No .: 67-63-0
상기의 시약을 혼합하여 디메틸페놀 용액을 조성하였고 시약 별 조합 구성비는 하기와 같다.
The reagents were mixed to form a dimethylphenol solution, and the composition ratio of each reagent was as follows.
따라서 상기한 실시예에서 보는 것처럼 포스포릭산 용액 또는/및 디메틸페놀 용액로 구성된 흡광광도법 또는 비색표대비법에 이용되는 질산성 질소 농도 검출시약을 제공하게 된다.Therefore, as shown in the above embodiment, the present invention provides a nitrate nitrogen concentration detection reagent used in the absorbance method or the colorimetric contrast method composed of a phosphoric acid solution and / or a dimethylphenol solution.
또한 상기한 질산성 질소 농도 검출시약을 포함하는 수질측정 검출키트를 제공한다.
In addition, the present invention provides a water quality detection kit comprising the above nitrate concentration detection reagent.
상기한 바와 같이 본 발명에 따른 질산성 질소 농도를 검출하는 검출시약을 이용하여 질산성 질소 농도를 측정하는 경우 시료 중에 포함된 다른 종류의 이온에 의한 간섭(Interfere)의 영향을 배제할 수 있는 한계치를 조사한 결과 다음 표와 같은 현저한 효과가 있는 결과를 얻을 수 있었다.
As described above, in the case of measuring the nitrate nitrogen concentration using the detection reagent for detecting the nitrate nitrogen concentration according to the present invention, a limit value that can exclude the influence of interference by other kinds of ions contained in the sample. As a result of the investigation, the results with remarkable effects as shown in the following table were obtained.
상기와 같이 하여 질산성 질소 농도를 검출하는 검출시약을 제공하게 된다.As described above, a detection reagent for detecting nitrate nitrogen concentration is provided.
또한, 본 발명은 상기한 검출시약을 포함하는 수질측정 검출키트를 제공하게 된다.
In addition, the present invention provides a water quality detection kit comprising the above-described detection reagent.
본 발명은 수질환경기본법과 관련된 정수 및 하폐수처리사업, 공해방지업, 교육사업 등에 널리 사용가능하다.The present invention can be widely used in water purification and sewage water treatment business, pollution prevention business, education business, etc. related to the Framework Act on Water Environment.
또한 본 발명은 공해방지모니터링업, 국가 및 지방정부의 환경정책관련업 등에 널리 이용가능하다.In addition, the present invention can be widely used for pollution prevention monitoring industry, environmental policy related business of national and local government.
이와 더불어, 수질분석약품사업, 수질분석약품제조업, 환경감리업, 환경모델링업, 환경영향평가업 및 교육용 자료 제공업 등에 매우 유용한 발명이다.
In addition, it is a very useful invention for water quality analysis chemicals business, water quality analysis chemical manufacturing industry, environmental supervision business, environmental modeling business, environmental impact assessment business and educational data providing business.
Claims (4)
포스포릭산 용액 또는 디메틸페놀 용액 중 하나 이상으로 구성한 질산성질소 농도 검출시약.
In the reagent for measuring the nitric acid concentration,
Nitrogen nitrate concentration detection reagent consisting of at least one of a phosphoric acid solution or a dimethylphenol solution.
포스포릭산 용액은 증류수, Phosphoric Acid, Sulfuric Acid를 혼합하여 조성하고,
디메틸페놀 용액은 증류수, dimethylphenol, Isopropanol을 혼합하여 조성한 것을 특징으로 하는 질산성질소 농도 검출시약.
The method of claim 1,
Phosphoric acid solution is prepared by mixing distilled water, Phosphoric Acid, Sulfuric Acid,
Dimethyl phenol solution is a distilled water, dimethylphenol, isopropanol mixed reagent composition characterized in that the composition is composed.
포스포릭산 용액은 증류수 100중량부에, Phosphoric Acid 200~400중량부, Sulfuric Acid400~800중량부를 혼합하여 조성하고,
디메틸페놀 용액은 증류수 100중량부에, dimethylphenol 0.5~4중량부, Isopropanol 15~35중량부 혼합하여 조성한 것을 특징으로 하는 질산성질소 농도 검출시약.
The method of claim 2,
Phosphoric acid solution is prepared by mixing 100 parts by weight of distilled water, 200 to 400 parts by weight of Phosphoric Acid, 400 to 800 parts by weight of sulfuric acid,
The dimethylphenol solution is a nitrogen nitrate concentration detection reagent, characterized in that the composition is mixed with 100 parts by weight of distilled water, 0.5 to 4 parts by weight of dimethylphenol, 15 to 35 parts by weight of Isopropanol.
A water quality detection kit comprising the reagent for detecting nitrogen nitrate concentration according to any one of claims 1 to 3.
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| KR101057970B1 (en) | 2011-04-18 | 2011-08-19 | 길주형 | The analyzer of inorganic compounds nitrogen into the water |
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