KR101193099B1 - Paper Mask Contained with the Artemisia Extract Killing the 2009AH1N1 Influenza Viruses and the H9N2 Avian Influenza Viruses - Google Patents

Abstract

The present invention relates to a paper mask containing a wormwood organic solvent extract having an inactivation ability against the swine flu virus and avian influenza virus, which will be described in detail, the fine dust barrier, breathability, warmth, moisture of the traditional paper The paper mask is constructed by using the absorbent property. The paper mask is made of 1 sheet by laminating two sheets of paper so that they are perpendicular to each other in the horizontal and vertical directions in the wetland state, thereby improving the binding force. It contains organic solvent extract or sprays the extract of organic solvent on the inner side of paper mask to have antimicrobial and antiviral functions such as avian influenza and swine flu. By putting the mask knob in the center of symmetry And easy to use to make up your disc, to be decorated with embossed, cubic, hatpiseu, pattern, including a certain portion of the outer fingers of the paper masks on a paper mask, wherein configured to have an effective fashion.

Description

Paper Mask Contained with the Artemisia Extract Killing the 2009A (H1N1) Influenza Viruses and the H9N2 Avian Influenza Viruses}

The present invention relates to a paper mask containing a wormwood organic solvent extract having an inactivation ability against the swine flu virus and avian influenza virus, which will be described in detail, the fine dust barrier, breathability, warmth, moisture of the traditional paper A paper mask is constructed by using the absorbent properties. The paper mask is made of one piece by laminating two pieces of paper (wet paper) at right angles to each other in the horizontal and vertical directions to improve the binding force. It contains organic solvent extracts or sprays the extracts of the wormwood on the inner side of the paper mask to have the antimicrobial and antiviral functions such as bird flu and swine flu. Mask by placing the mask knob in the center of symmetry Conveniently used to make up time, and to be decorated by embossing, cubic, hatpiseu, patterns such as the constant portion of the outer fingers of the paper mask on the mask, characterized in that paper is configured to have a fashion effect.

Conventionally, masks are worn by the general public to prevent seasonal or infectious diseases, and are intended to prevent various pathogens from outside from being infected through the air or to release pathogens present in the patient's body to the outside. Used as.

In addition, when a doctor or a nurse in a hospital treats a patient or a patient concerned about bacterial infection in an operating room and a treatment room, the doctor or a nurse wears it in order to prevent infection of the surgical site and the treatment site of the patient.

Such a conventional mask is developed as a cotton mask, a nonwoven mask, and the like. Cotton masks are less breathable and moisture absorbent, and non-woven masks are not good for atopic skin.

In order to improve the problems of conventional cotton masks and nonwoven masks, it is necessary to develop a mask made of a traditional paper that is excellent in breathability and moisture absorption as a natural material and has no hypersensitivity to atopic skin.

In particular, Korean traditional paper, Hanji, and Japanese traditional paper, Hwaji, are commonly made from bast fibers and hemp from paper mulberry, anpi, and cedar. In addition, traditional Chinese paper and bamboo paper are made from various kinds of bamboo bark, rice straw and straw. In addition, India makes traditional paper from abrasion materials, and traditional paper continues to be used in Europe and the United States, and the traditional paper is masked, and its durability is poor because the paper is easily torn.

In order to solve the above problems, the paper mask containing the extract of the wormwood organic solvent of the present invention is a paper using a Korean traditional paper made from bast fiber and hemp from the mulberry, anarch, cedar By manufacturing the mask, by using the toughness of the paper, two sheets were laminated at right angles in the horizontal and vertical directions to manufacture the mask, and thus the binding strength was improved. These traditional papers have excellent air permeability and warmth when compared to cotton fibers. It is hygienic because of its excellent moisture absorption, good wearability, and excellent antibacterial and ammonia deodorization rate. Hygiene and far-infrared (5 ~ 20㎛) emissivity are average 0.2. Help more. There is also research showing that traditional paper is good for atopic skin. Therefore, it is a good reason to use these traditional papers as masks.

In 2009, the swine flu epidemic began, causing many deaths worldwide. Thus, the use of masks for the prevention of the H1N1 flu has increased all over the world, and still does not solve such fears.

Therefore, in order to overcome this problem, the swine flu by the method of containing or spraying the mugwort organic solvent extract to the paper mask by adding a wormwood organic solvent extract that kills the swine flu virus and avian influenza virus to the paper mask. The aim was to provide a paper mask that would be effective in preventing a new form of strain of influenza in combination with a possible bird flu virus.

The paper mask is a disposable mask and is an environmentally friendly mask of natural extracts, and provides a new concept of traditional paper masks that not only prevents or protects from diseases, but also uses a fashion tool that expresses itself by breaking away from the concept of a general mask. .

The present invention relates to a traditional paper mask containing an extract of the wormwood organic solvent having an inactivation ability against the swine flu virus and avian influenza virus, fine dust-blocking, breathable , Warmth, moisture absorption, high far-infrared emissivity, antiviral effect of wormwood organic solvent extract, convenience of raising and lowering mask by putting mask handle in the center of left and right symmetry of paper mask and traditional paper mask It has the effect of fashioning yourself in various ways.

1 is a flow chart of the production of traditional paper and paper mask production
2 is a block diagram of a traditional paper mask
3 is another embodiment of the traditional paper mask configuration
4 is another embodiment configuration of a traditional paper mask
5 is a certificate for inactivation of swine flu (H1N1) virus of wormwood extract of organic solvent

The present invention relates to a paper mask containing a wormwood organic solvent extract having an inactivation ability against the swine flu virus and avian influenza virus, which will be described in detail, the fine dust barrier, breathability, warmth, moisture of the traditional paper A paper mask is constructed by using the absorbent properties. The paper mask is made of one piece by laminating two pieces of paper (wet paper) at right angles to each other in the horizontal and vertical directions to improve the binding force. It contains organic solvent extract or sprays the wormwood organic solvent extract on the inner side of paper mask to have antibacterial and antiviral functions against bird flu and swine flu. Mask by placing the mask knob in the center of symmetry A conveniently used when up to down and to be decorated by embossing, cubic, hatpiseu, patterns such as the constant portion of the outer fingers of the paper mask on the mask, characterized in that paper is configured to have a fashion effect.

Hereinafter, the configuration of the present invention will be described in more detail with reference to the following examples, but the scope of the present invention is not limited only to the following examples, and various modifications can be made without departing from the scope and spirit of the present invention. Is possible.

The following is described with reference to their results through Examples and Experimental Examples as follows.

[ Example 1 Traditional Paper Mask Manufacturer

In general, in the manufacture of traditional paper, if you want to get thick paper, you can make several sheets of wetland floating one by one. In other words, calligraphy papers use 1 sheet of paper with a wetland average thickness of about 1mm, and the door window has to be breathable and durable. To make it even higher, we use three laminations made of three overlapping wetlands. However, jangpanji has a wetland average thickness of about 3 mm or more, so there is little ventilation. The traditional method of laminating paper is to place the paper on one side of the material and the wetland in the opposite direction. This is to make paper thicker and to increase the durability of the paper.

In addition, the material of the traditional paper mask is a paper, that is, using traditional paper such as Hanji, Japanese paper, porridge, and paper, the first step is to make a traditional paper with good durability and breathability. The production of traditional paper consists of papermaking, wetland stacking, pressing, drying and glue processing.

As such, traditional paper has many advantages when used as a mask compared to cotton fiber, but to compensate for the fact that the paper is easily torn and poor in durability,

In the present invention, in order to make a traditional paper mask with both good durability and breathability, the paper making process is different from the paper making process, first, the wetland floats very thin so that the average thickness is 0.5mm. Then, on a very thin wetland, they join each other at right angles in the horizontal and vertical directions, rather than in opposite directions. In this way, the thickness is as thin as ordinary traditional paper, and the durability is stronger than that of conventional two paper. In other words, in the case of traditional paper, since the grain is oriented in one direction and easily torn, the durability is low. By manufacturing a paper in which the grain is laminated horizontally and vertically, the paper is durable and breathable in both horizontal and vertical directions. This good paper is born.

1 shows a flowchart of the production of traditional paper and the manufacture of a paper mask,

First, the first step is the production of traditional paper, the production of traditional paper consists of the process of papermaking, wetland stacking, pressing, drying, glue processing. When making wetlands, traditional paper is made by laminating them horizontally and vertically.

The second stage cuts the traditional paper into the mask size.

In the third step, the outer paper 1 of the traditional paper mask A may be embossed, cubic, hotpiece, and decorative parts 4 such as patterns to fashion.

In the fourth step, the earring part 3 is attached to a predetermined portion where the outer side paper 1 and the inner side paper 2 of the traditional paper mask A are laminated to be processed into a traditional paper mask.

The last five steps are spraying wormwood organic solvent extract or natural medicinal dye on traditional paper.The wormwood organic solvent extract used has 99.99% inactivation ability against H1N1 virus and disinfection effect against avian influenza virus. The traditional paper mask is completed by containing the extract of the organic solvent in the inner paper (2) inside the paper mask (A).

Figure 2 is a block diagram of a paper mask of the present invention, to make a paper mask using a traditional paper, the inner paper (2) provided with a wormwood organic solvent extract inside the paper mask (A), and It is a paper mask which consists of the outer side paper 1 provided in the outer side of the mask A, and the hook part 3, and FIG. 3 shows another implementation block diagram of the traditional paper mask, The outer side of the paper mask A It is a paper mask fashioned by placing decorative parts 4 such as embossing, cubic, hot pieces, and patterns on a certain part of the paper (1). In addition, the binding part 5 of the paper mask shows that the inner side paper 2 and the outer side paper 1 of the paper mask A were needle-moistened with colored threads.

Figure 4 shows another embodiment configuration of the traditional paper mask, which is configured to be conveniently used to raise and lower the mask by placing the mask handle 6 in the center of the left and right symmetry of the outer paper (1) of the paper mask Indicates.

[ Example  2] Preparation of Mugwort Organic Solvent Extract

The paper mask (A) was configured to form a traditional paper mask by incorporating or spraying the influenza influenza virus and extracts of organic solvent extracts of natural herbs having a disinfection effect against avian influenza virus.

The following is the step of preparing organic solvent extract of wormwood in Cheonnyeonji Co., wormwood extract crushed dried leaves of the leaves or wormwood with a grinder to precipitate for 24 to 48 hours in 95% alcohol alcohol, pharmacological components of wormwood After extracting enough alcohol alcohol, the mugwort leaves and impurities are removed, and the alcoholic alcohol is volatilized, the mugwort extract remains. Based on the mugwort extract, the same amount of emulsifier was added, and then diluted and used with distilled water of 98 wt. Times.

The following shows the experiment and results of the inactivation of the swine flu (H1N1) of the extract of the wormwood organic solvent.

[ Experimental Example  1] Extract of Mugwort Organic Solvent Extract Influenza A (H1N1)  Experiment of inactivation ability for

1. Purpose and Background of Test

The purpose of this study was to analyze the inactivation of the H1N1 virus virus of mugwort (apple and wormwood) extract commissioned by Chun, Yon-Ae (CEO: Professor Bong-Kwan Cho, Pukyong National University). The following results were obtained through the service research through).

According to the above results, the traditional paper mask of the present invention was prepared by adding a mugwort extract which is a substance that inactivates the influenza virus, and relates to a paper mask containing the extract of the organic solvents of the wormwood.

For experimental background, swine flu (H1N1) influenza originated from pigs began spreading worldwide in Mexico and the United States in early April 2009. The World Health Organization (WHO) has already declared a pandemic, has killed more than 10,000 people, lacks a complete cure, and lacks a vaccine to prepare. Therefore, the H1N1 inactivation verification of harmless organic solvent extracts of wormwood, which may be harmful to the human body, may greatly help personal hygiene.

 2. Measuring method

The sample of Mugwort (Apricot or Mugwort) organic solvent extract was used for analysis from the Millennium Co., Ltd., and the target virus of the extract of the mugwort organic solvent was a pandemic (H1N1) virus, which was declared as a pandemic. / O4 / 09 (H1N1)) was used as a method of measurement: 1) 100 μl of Swine Flu (H1N1) 10 ⁶ EID ₅₀ / ml (50% egg infection amount) was applied to 100 μl of the extract of S. aureus. . 2) React at room temperature for 20 minutes. 3) After reacting for 20 minutes, dilute serially with PBS 10 times. 4) Inoculate 10-fold diluted solution into 10-day-old fertilized eggs. 5) The inoculated fertilized eggs are incubated in an incubator at 35 ° C. for 48 hours and then refrigerated in a refrigerator at 4 ° C. for 4 hours. 6) The presence of virus in the ureters of chilled fertilized eggs is measured by hemagglutination, and log 10 egg infectious dose 50 / ml (1og ₁₀ EID ₅₀ / ml) is measured.

[Experiment result]

As shown in Table 1 as a result of inactivation of the H1N1 influenza extract of the organic solvent extract of the wormwood, it was confirmed that Cheonnyeonae wormwood organic solvent extracts inactivate the H1N1 virus to 99.99% .

The following shows the experiment and results of the antiseptic effect of the extract of wormwood organic solvents against avian influenza virus.

[ Experimental Example  2] Disinfection Efficacy of Mugwort Organic Solvent Extract against Avian Influenza Virus

1. Purpose and Background of Test

 The purpose of this study was to analyze the disinfection efficacy of avian influenza virus by using three kinds of extracts of the wormwood organic solvents B, C, and D, commissioned by Cheonnyeon Jiae Co., Ltd. The following results were obtained through the service research through the professor.

Referring to the three types of mugwort extract B, C, D, mugwort extract B is crushed by drying the leaves of the leaves or wormwood with a grinder, to obtain a methanol extract of the mugwort using a methyl alcohol solvent, ethyl from the methanol extract of the mugwort Using the acetate solvent, fractions of ethyl acetate acetate fractions were fractionated to obtain organic solvent extracts of mugwort. Based on mugwort extract, 3 weight-fold emulsifier was added to prepare stock solution. Mugwort extract C was boiled in water to break the wormwood, and the mugwort extract was extracted in a semi-solid state as crude syrup as a stock solution. Mugwort extract D was used to add the same amount of the emulsifier on the basis of the extract of the mugwort organic solvent in the step of obtaining the same mugwort organic solvent extract in the acquisition process of the mugwort extract B.

The target pathogen used was avian influenza virus A / chicken / Korea / MS96 / 1996 (H9N2), and diluting folds were used by diluting three types of mugwort extract stocks B, C and D 100 times, 200 times, 400 times and 800 times respectively. The test method was conducted in accordance with the antimicrobial efficacy test regulations of Annex 2 of the Guidance for Disinfectant Effectiveness Test of Notification of Veterinary Research and Quarantine Service 2007-10.

2. Disclosure Virus

1) Virus: In order to evaluate the disinfection effect of the virus on the test disinfectant, avian influenza virus H9N2 (MS96 strain), which was distributed and stored by the National Veterinary Research and Quarantine Service, was provided for the test.

2) Virus culture: MS96 strain of avian influenza virus was cultured in 9-day-old SPF embryonated eggs, and the virus was grown to a minimum titer of 10 ^7.0 EID ₅₀ / ml, and the proliferated virus was maintained at minus 70 ℃. Used while storing in.

3. Test method

1) Dilution of disinfectant: Each dilution of test disinfectant was diluted with organic dilution (5% fetal bovine serum), and the dilution times were 1/100, 1/200, 1/400 and 1/800 times of the original solution.

2) Disinfectant reaction: Prepare 1.0ml virus for disinfectant reaction by mixing 1.0ml of virus solution (urine membrane solution) grown at 4 ℃ with 19ml of organic diluent (5% fetal bovine serum) at 4 ℃. 2.5 ml of each prepared virus solution was added to a test tube containing the same amount of disinfectant diluent at 4 ° C., respectively (total 5 ml), and reacted at 4 ° C. for exactly 30 minutes, and shaken well every 10 minutes. The control group used hard water instead of disinfectant solution.

3) Neutralization: After the reaction of the antiseptic agent was finished, 1.0 ml was immediately taken out to neutralize the efficacy of the disinfectant and mixed with PBS at 37 ° C. containing the same amount of unassisted 10% fetal bovine serum.

4) Determination of virus propagation: Neutralizing solution was diluted with stock solution, 10 ^-1 , 10 ^-2 , 10 ^-3 , 10 ^-4 , 10 ^-5 using PBS, and 0.2ml of 5 10-day-old embryonated eggs per dilution. It was inoculated into urine membrane and incubated for 5 days in 37 degreeC incubator. Embryo which died within 24 hours after vaccination during the day of inoculation was considered as an accident and excluded from test results.

5) Evaluation of Effective Disinfectant Concentration: Diluted concentrations with inactivation of 10 ^4.0 EID ₅₀ / ml or more were considered as effective concentrations by comparing the virus content of the disinfectant treatment group with the virus content of the pathogen control group.

The virus content was calculated using Karber method.

[Experiment result]

As shown in the results of the disinfection efficacy test according to the disinfectant dilution multiple for the avian influenza virus of the mugwort extract of Table 2, the result of testing the disinfection effect against the avian influenza virus for each extract of the mugwort extract 100 times the stock solution Disinfection effect was recognized in (1/100) diluent, and disinfection effect was not recognized in 100 times (1/100) dilution of stock solution in Formulation C, and to 200 times (1/200) dilution concentration of stock solution in D formulation. Disinfection effect was recognized.

According to the results of the above experiments, the extracts of the wormwood used in the present invention were inactivated swine flu (H1N1) virus (Table 1 and FIG. 5) and disinfection effect against avian influenza virus (Table 2). It became.

A: Paper Mask
1: outside sheet of paper mask 2: inside sheet of paper mask
3: Earring part of the paper mask 4: Decorative part of the paper mask
5: binding portion of the paper mask 6: handle of the paper mask

Claims (6)

A paper mask is made using Korean paper, and an inner sheet (2) provided with a wormwood organic solvent extract inside the paper mask (A), and an outer sheet (1) and a hanger provided on the outer side of the mask (A) A paper mask composed of a section (3).
The paper mask (A) according to claim 1, wherein the inner side paper 2 and the outer side paper 1 are one sheet each, and two sheets of two sheets are laminated so that the grains are perpendicular to each other in the horizontal and vertical directions. Paper mask, characterized in that consisting of one sheet.
The paper mask according to claim 1, wherein the paper mask is provided with a mask handle (6) at the center of left and right symmetry of the outer sheet (1).
The paper mask according to claim 1, wherein the paper mask is configured to re-inject the organic solvent extract of Mugwort into the mask inner paper (2).
The paper mask of claim 1, wherein the extract of the wormwood organic solvent used in the paper mask (A) has an inactivation ability against the H1N1 virus and a disinfection effect against the avian influenza virus.
The paper mask according to claim 1, wherein the paper mask (A) consists of at least one decorative part (4) selected from embossing, cubic, hot piece, and pattern on a portion of the outer sheet (1) of the mask.

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