KR101190586B1 - Experimental apparatus for culturing a microorganism by light - Google Patents

Experimental apparatus for culturing a microorganism by light Download PDF

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KR101190586B1
KR101190586B1 KR20100046226A KR20100046226A KR101190586B1 KR 101190586 B1 KR101190586 B1 KR 101190586B1 KR 20100046226 A KR20100046226 A KR 20100046226A KR 20100046226 A KR20100046226 A KR 20100046226A KR 101190586 B1 KR101190586 B1 KR 101190586B1
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light
photosynthetic
photoculture
temperature
experiment apparatus
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KR20110126774A (en
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이계안
김기용
정민호
조월순
정경진
오영섭
장희만
고수근
양우성
채선홍
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(주)엔엘피
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00—Means for providing, directing, scattering or concentrating light
    • C12M31/02—Means for providing, directing, scattering or concentrating light located outside the reactor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/14—Incubators; Climatic chambers

Abstract

본 발명은 광합성 미생물 광배양 실험장치에 대한 것으로서, 더욱 상세하게는 광합성 미생물을 광배양하기 위하여 최적의 조건을 찾을 수 있는 광합성 미생물 광배양 실험장치에 대한 것이다.
본 발명에 따른 광합성 미생물 광배양 실험장치는 온도센서와, 온도조절수단과, 발광부와, 제어부를 포함한다. 상기 온도센서는 광합성 미생물을 배양하기 위하여 광합성 미생물이 저장된 플라스크가 수용되는 수용실의 온도를 측정한다. 상기 온도조절수단은 상기 수용실의 온도를 조절한다. 상기 발광부는 복수의 적색조명과, 복수의 청색조명을 구비하며 상부에 상기 플라스크가 안착된다. 상기 제어부는 상기 발광부를 점멸할 수 있고, 상기 적색조명과 상기 청색조명의 광량 및 광 비율을 조절한다.
본 발명에 의하면 광량과 광 파장 비율 및 발광부의 점멸 및 연속식으로 조사할 수 있는 광합성 미생물 광배양 실험장치를 제공함으로써 광합성 미생물 배양에 적합한 최적의 조건을 파악할 수 있다.The present invention relates to a photosynthetic microbial photoculture experiment apparatus, and more particularly, to a photosynthetic microbial photoculture experiment apparatus capable of finding optimal conditions for photoculturing photosynthetic microorganisms.
The photosynthetic microbial photoculture experiment apparatus according to the present invention includes a temperature sensor, a temperature control unit, a light emitting unit, and a control unit. The temperature sensor measures the temperature of the chamber containing the flask in which the photosynthetic microorganisms are stored in order to culture the photosynthetic microorganism. The temperature control means adjusts the temperature of the storage chamber. The light emitting unit includes a plurality of red lights and a plurality of blue lights, and the flask is seated thereon. The control unit may blink the light emitting unit and adjust the light quantity and the light ratio of the red light and the blue light.
According to the present invention, by providing a photosynthetic microbial photoculture experiment apparatus capable of irradiating light quantity and light wavelength ratio and flashing and continuous light emission, it is possible to determine the optimum conditions suitable for photosynthetic microbial culture.

Description

광합성 미생물 광배양 실험장치{Experimental apparatus for culturing a microorganism by light}Experimental apparatus for culturing a microorganism by light}

본 발명은 광합성 미생물 광배양 실험장치에 대한 것으로서, 더욱 상세하게는 광합성 미생물을 광배양 하기 위하여 최적의 조건을 찾을 수 있는 광합성 미생물 광배양 실험장치에 대한 것이다.The present invention relates to a photosynthetic microbial photoculture experiment apparatus, and more particularly, to a photosynthetic microbial photoculture experiment apparatus capable of finding optimal conditions for photoculturing photosynthetic microorganisms.

녹색성장과 신재생 에너지라는 세계적 흐름에 맞추어 최근 미세 조류 배양을 통한 이산화탄소 저감 및 바이오(Bio) 에너지, 유효 물질 생산에 관한 연구가 주목 받고 있다.In line with the global trend of green growth and renewable energy, research on carbon dioxide reduction, bio energy and active substance production through microalgae cultivation has attracted attention.

미세 조류를 대량으로 배양하기 위하여 다양한 종에 적합한 맞춤형 배양 방식의 도입이 필요하다. 이를 위하여 미세 조류 연구에 있어서 다양한 미세 조류의 생태적인 특성 및 최적의 배양 조건의 확립이 필수적이다. 이를 위하여 광합성 미생물을 배양하기 위한 광실험장치가 사용된다.In order to cultivate microalgae in large quantities, it is necessary to introduce a customized culture method suitable for various species. For this purpose, it is essential to establish the ecological characteristics and optimum culture conditions of various microalgae in microalgae research. For this purpose, a light experiment apparatus for culturing photosynthetic microorganisms is used.

도 3은 종래의 광합성 미생물 광배양 실험장치이다. 종래의 광합성 미생물 광배양 실험장치는 형광등(3)을 사용하여 광실험을 한다. 즉 배양할 광합성 미생물이 저장된 플라스크(1)를 수용하는 수용실에 다수의 형광등(3)이 설치된다. 그래서 수용실 내부에 광합성 미생물이 저장된 플라스크(1)를 수용시킨 후 형광등(3)으로 광을 조사시켜 광배양 실험을 하였다.Figure 3 is a conventional photosynthetic microbial photoculture experiment apparatus. Conventional photosynthetic microbial photocultivation experiment apparatus is a light experiment using a fluorescent lamp (3). That is, a large number of fluorescent lamps 3 are installed in the storage chamber that houses the flask 1 in which the photosynthetic microorganisms to be cultured are stored. Therefore, after receiving the flask (1) storing the photosynthetic microorganism inside the storage chamber was irradiated with a fluorescent lamp (3) to perform a photoculture experiment.

종래의 광합성 미생물 광배양 실험장치는 형광등 만을 사용하였으므로 광합성 미생물의 광합성 배양에 적합한 최적의 광 조건을 찾을 수 없다는 문제점이 있었다.Since the conventional photosynthetic microbial photoculture apparatus used only a fluorescent lamp, there was a problem in that it was not possible to find an optimal light condition suitable for photosynthetic culture of photosynthetic microorganisms.

또한, 종래의 광합성 미생물 광배양 실험장치는 다양한 종에 따른 개별적인 광 선호 특성을 맞출 수 없다는 문제점이 있었다.In addition, the conventional photosynthetic microbial photoculture apparatus has a problem that can not match the individual light preference characteristics according to various species.

또한, 종래의 광합성 미생물 광배양 실험장치는 광합성 미생물의 배양에 적합한 최적의 온도 조건을 찾을 수 없다는 문제점이 있었다.In addition, the conventional photosynthetic microbial photoculture experiment apparatus has a problem that can not find the optimum temperature conditions suitable for the culture of photosynthetic microorganisms.

본 발명은 상기의 문제점을 해결하기 위한 것이다. 본 발명은 광합성 미생물의 광배양에 적합한 최적의 광조건을 찾을 수 있는 광합성 미생물 광배양 실험장치를 제공하는 것을 목적으로 한다.The present invention is intended to solve the above problems. An object of the present invention is to provide a photosynthetic microbial photocultivation experiment apparatus that can find the optimal photo conditions suitable for the photocultivation of photosynthetic microorganisms.

또한, 본 발명은 다양한 종에 맞추어서 광 선호 특성을 맞출 수 있는 광합성 미생물 광배양 실험장치를 제공하는 것을 목적으로 한다.In addition, an object of the present invention is to provide a photosynthetic microbial photoculture experiment apparatus capable of tailoring light preference characteristics to various species.

또한, 본 발명은 광합성 미생물의 배양에 적합한 최적의 온도 조건을 찾을 수 있는 광합성 미생물 광배양 실험장치를 제공하는 것을 목적으로 한다.In addition, an object of the present invention is to provide a photosynthetic microbial photoculture experiment apparatus that can find the optimal temperature conditions suitable for the culture of photosynthetic microorganisms.

본 발명에 따른 광합성 미생물 광배양 실험장치는 온도센서와, 온도조절수단과, 발광부와, 제어부를 포함한다. 상기 온도센서는 광합성 미생물을 배양하기 위하여 광합성 미생물이 저장된 플라스크가 수용되는 수용실의 온도를 측정한다. 상기 온도조절수단은 상기 수용실의 온도를 조절한다. 상기 발광부는 복수의 적색조명과, 복수의 청색조명을 구비하며 상부에 상기 플라스크가 안착된다. 상기 제어부는 상기 발광부를 점멸할 수 있고, 상기 적색조명과 상기 청색조명의 광량 및 광 비율을 조절한다.The photosynthetic microbial photoculture experiment apparatus according to the present invention includes a temperature sensor, a temperature control unit, a light emitting unit, and a control unit. The temperature sensor measures the temperature of the chamber containing the flask in which the photosynthetic microorganisms are stored in order to culture the photosynthetic microorganism. The temperature control means adjusts the temperature of the storage chamber. The light emitting unit includes a plurality of red lights and a plurality of blue lights, and the flask is seated thereon. The control unit may blink the light emitting unit and adjust the light quantity and the light ratio of the red light and the blue light.

본 발명에 의하면 광량과 광 파장 비율 및 발광부의 점멸 및 연속식으로 조사할 수 있는 광합성 미생물 광배양 실험장치를 제공함으로써 광합성 미생물 배양에 적합한 최적의 조건을 파악할 수 있다.According to the present invention, by providing a photosynthetic microbial photoculture experiment apparatus capable of irradiating light quantity and light wavelength ratio and flashing and continuous light emission, it is possible to determine the optimum conditions suitable for photosynthetic microbial culture.

도 1은 본 발명에 따른 광합성 미생물 광배양 실험장치의 일 실시예를 사용하여 광합성 미생물을 광배양 하는 사시도,
도 2는 도 1에 도시된 실시예의 개념도,
도 3은 종래의 광합성 미생물 광배양 실험장치의 개념도이다.1 is a perspective view of photoculture of photosynthetic microorganisms using one embodiment of a photosynthetic microorganism photoculture experiment apparatus according to the present invention;
2 is a conceptual diagram of the embodiment shown in FIG.
3 is a conceptual diagram of a conventional photosynthetic microbial photoculture experiment apparatus.

도 1 및 도 2를 참조하여 본 발명에 따른 광합성 미생물 광배양 실험장치의 일 실시예를 설명한다.1 and 2 will be described an embodiment of the photosynthetic microbial photoculture apparatus according to the present invention.

본 발명에 따른 광합성 미생물 광배양 실험장치는 온도센서(11)와, 온도조절수단(13)과, 발광부(15)와, 제어부(21)를 포함한다. 온도센서(11)는 광합성 미생물을 배양하기 위하여 광합성 미생물이 저장된 플라스크(1)가 수용되는 수용실(10)의 온도를 측정한다.The photosynthetic microbial photoculture experiment apparatus according to the present invention includes a temperature sensor 11, a temperature control unit 13, a light emitting unit 15, and a control unit 21. The temperature sensor 11 measures the temperature of the chamber 10 in which the flask 1 in which the photosynthetic microorganisms are stored is accommodated in order to culture the photosynthetic microorganisms.

온도조절수단(13)은 수용실(10)의 온도를 조절한다. 온도센서(11) 및 온도조절수단(13)을 사용하여 광합성 미생물이 배양되는 최적의 온도 조건을 파악할 수 있다.The temperature regulating means 13 adjusts the temperature of the storage chamber 10. The temperature sensor 11 and the temperature control means 13 can be used to determine the optimal temperature conditions for photosynthetic microorganisms to be cultured.

발광부(15)는 복수의 LED로 된 적색조명(17)과 복수의 LED로 된 청색조명(19)을 구비하며 상부에 플라스크(1)가 안착된다. 발광부(15) 위에 광합성 미생물이 저장된 플라스크(1)를 안착시켜 플라스크(1)에 광을 조사시킨다.The light emitting unit 15 includes a red light 17 made of a plurality of LEDs and a blue light 19 made of a plurality of LEDs, and a flask 1 is mounted thereon. The flask 1, on which the photosynthetic microorganisms are stored, is placed on the light emitting unit 15 to irradiate the flask 1 with light.

제어부(21)는 발광부(15)를 일정한 시간 간격으로 점멸시키거나 또는 연속적으로 작동시킬 수 있다. 그리고 제어부(21)는 발광부(15)의 적색조명(17)과 청색조명(19)의 광량 및 광 비율을 조절할 수 있다. 즉 제어부(21)는 플라스크(1)에 광을 연속적으로 조사하거나 또는 일정한 시간 간격으로 점멸하여 조사할 수 있다. 그래서 광합성 미생물을 배양하기 위하여 보다 효율적인 에너지 전달 방식을 찾을 수 있다. 또한, 제어부(21)는 플라스크(1)에 조사되는 적색조명(17)과 청색조명(19)의 비율을 조절할 수 있다. 예를 들면 청색광과 적색광의 비율을 10:0, 5:5, 7:3, 3:7, 0:10 등의 비율로 조절할 수 있다. 그리고 제어부(21)는 플라스크(1)에 조사되는 광량의 세기를 조절할 수 있다. 이를 통하여 광합성 미생물의 배양에 필요한 최적의 조건을 찾을 수 있다.The control unit 21 may blink or continuously operate the light emitting unit 15 at regular time intervals. The controller 21 may adjust the light quantity and the light ratio of the red light 17 and the blue light 19 of the light emitting unit 15. That is, the control unit 21 may irradiate the flask 1 continuously with light or blink at regular time intervals. Therefore, a more efficient energy transfer method can be found for culturing photosynthetic microorganisms. In addition, the controller 21 may adjust the ratio of the red light 17 and the blue light 19 irradiated to the flask 1. For example, the ratio of blue light and red light can be adjusted in a ratio of 10: 0, 5: 5, 7: 3, 3: 7, 0:10 and the like. And the control unit 21 may adjust the intensity of the light amount irradiated to the flask (1). Through this, it is possible to find the optimal conditions for culturing photosynthetic microorganisms.

본 실시예의 광합성 미생물 광배양 실험장치를 사용하면 청색조명과 적색조명의 다양한 광파장 비율과, 다양한 광량과, 수용실의 다양한 온도 조건 그리고 다양한 점멸 시간에 대하여 광합성 미생물 광배양 실험을 할 수 있다. 이를 통하여 다양한 종에 대하여 광합성 미생물을 배양하기 위한 최적의 조건을 찾아낼 수 있다.Using the photosynthetic microbial photoculture apparatus of the present embodiment, it is possible to perform photosynthetic microbial photocultivation experiments for various light wavelength ratios, various light amounts, various temperature conditions of the storage chamber, and various flashing times. Through this, the optimum conditions for culturing photosynthetic microorganisms for various species can be found.

1 : 플라스크 3 : 형광등
10 : 수용실 11 : 온도센서
13 : 온도조절수단 15 : 발광부
17 : 적색조명 19 : 청색조명
21 : 제어부1: flask 3: fluorescent lamp
10: accommodation room 11: temperature sensor
13 temperature control means 15 light emitting unit
17: red light 19: blue light
21: control unit

Claims (1)

광합성 미생물을 배양하기 위하여 광합성 미생물이 저장된 플라스크가 수용되는 수용실의 온도를 측정하기 위한 온도센서와,
상기 수용실의 온도를 조절하기 위한 온도조절수단과,
복수의 적색조명과, 복수의 청색조명을 구비하며 상부에 상기 플라스크가 안착될 수 있는 발광부와,
상기 발광부를 점멸할 수 있고, 상기 적색조명과 상기 청색조명의 광량 및 광 비율을 조절할 수 있는 제어부를 포함하는 것을 특징으로 하는 광합성 미생물 광배양 실험장치.A temperature sensor for measuring the temperature of a storage chamber in which the flask containing the photosynthetic microorganisms is stored in order to culture the photosynthetic microorganisms;
Temperature control means for adjusting the temperature of the accommodation chamber;
A light emitting unit having a plurality of red lights and a plurality of blue lights and having the flask seated thereon;
And a control unit capable of blinking the light emitting unit and controlling a light quantity and a light ratio of the red light and the blue light.
KR20100046226A 2010-05-18 2010-05-18 Experimental apparatus for culturing a microorganism by light KR101190586B1 (en)

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Cited By (1)

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Publication number Priority date Publication date Assignee Title
KR20190113179A (en) 2018-03-28 2019-10-08 김동호 Device for testing the light stress

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KR102083164B1 (en) * 2018-10-12 2020-03-02 주식회사 비트윈 Device for testing the light stress

Citations (1)

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Publication number Priority date Publication date Assignee Title
US20100035321A1 (en) * 2007-04-20 2010-02-11 Bionavitas, Inc. Systems, devices, and, methods for releasing biomass cell components

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100035321A1 (en) * 2007-04-20 2010-02-11 Bionavitas, Inc. Systems, devices, and, methods for releasing biomass cell components

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20190113179A (en) 2018-03-28 2019-10-08 김동호 Device for testing the light stress

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