KR101173230B1 - The components of anti-obesity and its modulation of allium tuberosum rottl. extracts and processed sulfur - Google Patents

Abstract

PURPOSE: A composition containing Allium tuberosum rottl. juice freeze-dried material and processed sulfur is provided to prevent or treat obesity and to lower liver function. CONSTITUTION: A composition for preventing and treating arteroslcerosis contains 5-20 wt% of fermented Allium tuberosum rottl. juice freeze-dried material and 0.01-0.2 wt% of processed sulfur. The composition enhanced HDL-cholesterol level and lowers LDL-cholesterol level in serum. A health function food for preventing and treating arteroslcerosis contains the freeze-dried material and processed sulfur.

Description

Composition for preventing and improving obesity, comprising leek lyophilisate and legal sulfur {The components of anti-obesity and its modulation of Allium tuberosum rottl. extracts and processed sulfur}

The present invention relates to a composition for preventing and improving obesity, and more particularly, to a composition for preventing and improving obesity comprising leek juice lyophilisate and legal sulfur.

Obesity is generally a phenomenon in which the remaining portion of calories consumed is converted to fat and excessively accumulated in various parts of the body, especially the subcutaneous tissue or the abdominal cavity, and excessive accumulation affects normal physiological and biochemical functions. Attention has been drawn to the World Health Organization (WHO) and the world as a disease to treat obesity that can affect not only arthritis and cardiovascular diseases but also mental health. Recently, development of functional food materials using natural products such as flavonoids and chitosan that inhibit fat absorption and digestion for treating obesity, and capsaicin and catechin of green tea, which are contained in peppers that inhibit fat accumulation, is underway.

Leek ( Allium tuberosum rottl. ) Is a plant belonging to the genus Allium, and has been used as an herb by the unique aroma and taste of leek in Korea since ancient times. Leek contains sulfur-containing substances such as allyl compounds contained in garlic and onions belonging to the same Allium, and is rich in chlorophyll and dietary fiber, and contains adenosine (adenosin), an antithrombogenic component.

Sulfur has been used as a prescription for diseases such as hemostasis and nerve palsy after eliminating toxicity, and it is known to be effective in treating osteoporosis. have. Because sulfur is highly toxic, there is a high risk of side effects. Currently, non-toxic natural dietary sulfur (MSM, Methylsulfonylmethane) is used for food. At present, in order to produce functional duck meat, sulfur ducks are raised by using a mixture of sulfur and herbal medicine, and research on feeding dietary sulfur to pigs is being conducted little by little.

Therefore, the present inventors have studied the effects of leek and legal sulfur on body fat accumulation, and as a result, when preparing a composition containing leek juice lyophilisate and legal sulfur, unnecessary weight gain and body fat accumulation are prevented. It was confirmed that it can be prevented and improved obesity, thereby completing the present invention.

Therefore, the main object of the present invention is to provide a composition for preventing and improving obesity that can prevent and improve obesity by inhibiting unnecessary weight gain and body fat accumulation.

According to an aspect of the present invention, the present invention provides a composition for preventing and improving obesity comprising leek, leek juice or leek lyophilisate and legal sulfur.

In the composition of the present invention, the leek may be used as it is, but it is preferable to use the leek juice prepared by grinding and filtering the leek, more preferably the leek juice obtained after crushing and filtering the leek Freeze-dried leek juice is recommended to use.

In the composition of the present invention, the legal sulfur is to remove the toxicity of sulfur, the sulfur is added to the water to mature aged minerals, such as clay, and aged to remove the toxicity of sulfur and then manufactured by powdering the sulfur removed toxicity The sulfur content is preferably 99.0% or more. The legal sulfur can be easily manufactured or obtained by those skilled in the art to which the present invention pertains.

In the composition of the present invention, the leek juice lyophilisate is included in 5 to 20% by weight, the sulfur of the formulation is preferably contained in 0.01 to 0.2% by weight, more preferably the leek lyophilisate is 10 It is included in the weight%, the legal sulfur is preferably included in 0.87% by weight. It is determined based on the results of animal experiments, and it is a range of the preferred amount when the sulfur content of leek juice lyophilisate and legal sulfur is prepared to be 30 mg / 1 horses / day, based on the mouse, It is excellent in preventing and improving obesity.

The composition of the present invention has the effect of reducing weight, inhibiting the accumulation of peritesticular and peripheral fat, lowering the blood aspartate aminotransferase (AST) levels, and lowering the total lipid, triglyceride and total cholesterol levels of the liver.

According to another aspect of the present invention, the present invention provides a dietary supplement for preventing and improving obesity comprising the composition.

According to another aspect of the present invention, the present invention provides a functional livestock feed for preventing and improving obesity comprising the composition.

The composition of the present invention can be used for applications such as pharmaceuticals and food.

At this time, the composition according to the present invention can be formulated based on the formulation standard of the Food and Drug Administration (KFDA) as a conventional pharmaceutical formulation or the standard of the formulation of dietary supplements, preferably formulated for oral administration. .

The dosage of the present invention can be adjusted according to the condition, route of administration, and dosage form of the subject to be administered, and one of ordinary skill in the art can obviously use within various ranges, but in the present invention Through experiments, it was determined that an effective amount of 150 mg / kg body weight per day can be continuously or intermittently administered.

In addition, based on the effective amount of the composition of the present invention, there is provided a food comprising the composition of the present invention itself or a composition mixed with a food chemically acceptable carrier as a basic dietary material (其 本 食餌 素材), the food is meat Processed products, fish meat products, tofu, jelly, porridge, noodles such as ramen or noodles, seasoned foods such as soy sauce, miso, red pepper paste, mixed soy sauce, dairy products such as sauces, sweets, fermented milk or cheese, pickled foods such as kimchi and pickles, It can be used in the food of beverages such as fruits, vegetables, soy milk, fermented beverages. This is obvious to those skilled in the art of the present invention will not be described the specific cooking method or production method thereof. In addition, a pharmaceutically acceptable carrier may also be used as a pharmaceutically acceptable carrier.

As described above, according to the present invention, when the composition of the present invention is ingested, unnecessary weight gain and body fat accumulation can be suppressed, obesity can be prevented or improved, serum biochemical levels are stabilized, liver total lipids, triglycerides and Lowering your total cholesterol can improve liver function.

Figure 1 is a composition of the present invention, dietary fat, high fat diet, high fat diet containing legal sulfur, fermented leek juice lyophilisate and high fat diet containing sulfur (epididymal adipose tissue) of rats It is a graph showing the weight of the measurement. a, b, c means that there is a significant difference (p <0.05) when the alphabet is different, and all the values are expressed as the mean standard deviation (n = 5) (CON: general diet, HFD: high fat diet). Forage, HS: High-fat dietary foods containing legal sulfur (high-fat diet + legal sulfur), AS: Leek lyophilized and high-fat diets containing legal sulfur (high fat diet + leek lyophilis + legal sulfur ), FAS: Fermented leek juice lyophilised and high-fat diets containing legal sulfur (high fat diet + fermented leek juice lyophilised + legal sulfur).
Figure 2 of the composition of the present invention, general diet, high fat diet, high fat diet containing legal sulfur, fermented leek juice lyophilized and high fat diet containing sulfur containing the retroperitoneal fat of rats It is a graph that measured the weight. a and b mean that there is a significant difference (p <0.05) when the alphabets are different, and all values are expressed as mean ± standard deviation (n = 5).
Figure 3 shows the total lipid levels of the liver according to the composition of the present invention, diet, high fat diet, high fat diet containing legal sulfur, fermented leek juice lyophilisate and high fat diet containing legal sulfur. It is a graph measured and shown. a, b, and c mean that there is a significant difference (p <0.05) when the alphabets are different, and all values are expressed as mean ± standard deviation (n = 5).
Figure 4 shows the total cholesterol level of the rat liver according to the composition of the present invention, the diet, high fat diet, high fat diet containing legal sulfur, fermented leek lyophilized and high fat diet containing legal sulfur It is a graph measured and shown. a and b mean that there is a significant difference (p <0.05) when the alphabets are different, and all values are expressed as mean ± standard deviation (n = 5).
Figure 5 is the composition of the present invention, the general diet, high fat diet, high fat diet containing legal sulfur, fermented leek juice freeze-dried and the high fat diet containing the sulfur to measure the triglycerides (triglyceride) between rats The graph shown. a, b, and c mean that there is a significant difference (p <0.05) when the alphabets are different, and all values are expressed as mean ± standard deviation (n = 5).

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention, and the scope of the present invention is not to be construed as being limited by these examples.

Experimental Example 1

This experimental example is for confirming the weight loss and body fat reduction effect according to the composition of the present invention through an animal model.

As an animal model, a rat was used, and the composition of the present invention and the comparative composition were prepared in a feed form so as to be consumed by the rat.

1-1. Leek juice lyophilisate manufacturing

The leek was put in a green juice (Angel-juicer, Saha-gu, Busan) and pulverized and filtered with gauze (Korea Daejae Co., Ltd., Seo-gu, Daegu Metropolitan City) to prepare leek juice. The liquid medium was mixed at a 1: 1 ratio and then lyophilized with a freeze-drying machine (Ilshin Lab. MCFD 8510, Suwon, Gyeonggi-do). Mixing the strain inoculation medium is to compare the feed prepared by fermenting the leek juice was prepared by mixing the strain inoculation medium, but lyophilized without going through the culture and fermentation step.

1-2. Fermented leek juice lyophilized preparation

Using the same method as in 1-1 above, the strain inoculation medium was mixed and then incubated for 16 hours in a 30 ℃ incubator was added.

1-3. Sulfur content analysis

The sulfur content analysis of the lyophilized leek juice and fermented leek juice prepared in 1-1 and 1-2 were measured using EA 1110 (ThermoQuest CE INSTRUMENTS, Italia) analyzer without any pretreatment.

As a result, as shown in Table 1, the sulfur content of the non-fermented ones was significantly higher (p <0.05) than that of the fermented ones.

ATR FATR Sulfur content (%) 0.6 ± 0.02 ^a 0.4 ± 0.01 ^b

* ATR: leek juice lyophilisate, FATR: fermented leek juice lyophilisate

1-4. General Ingredient Analysis

Moisture, crude protein, crude fat, crude ash and crude fiber of lyophilized leek juice and fermented leek juice prepared in 1-1 and 1-2 were analyzed by AOAC method. Moisture at 105 ° C atmospheric pressure drying, ash at 550 ° C direct ashing, crude protein micro-Kjedahl method, crude fat soxhlet extraction method, crude fiber crude fiber extractor (Fibertherm, Gerhardt, USA), carbohydrate at 100, moisture, crude protein , Crude fat, crude ash and crude fiber were subtracted.

As shown in Table 2, crude protein and crude fat were significantly higher (p <0.05) than those that were not fermented.

ATR FATR moisture 0.1 ± 0.02 ^b 0.4 ± 0.06 ^a Ash 4.2 ± 0.12 4.4 ± 0.14 Crude protein 35.9 ± 0.72 ^a 34.5 ± 0.10 ^b Crude fat 0.7 ± 0.08a 0.5 ± 0.0b Crude fiber 0.1 ± 0.03 ^ND 0.1 ± 0.02 carbohydrate 58.9 ± 0.72 ^b 60.2 ± 0.09 ^a

* All values are expressed as mean ± standard deviation.

* a, b: If the alphabet is different, it means that there is a significant difference (p <0.05).

NS means no significance.

1-5. Feed preparation according to the composition of the present invention

To 100 g of the lyophilisate prepared in 1-1, 0.87 g of legal sulfur and 209.6 g of casein in high fat diet, 181.1 g of sucrose, 104.8 g of glucose (dextrose), 76.3 g of corn starch, 52.4 g of cellulose, 26.2 g of soybean oil, 186.0 g of lard, 47.2 g of mineral mixture, 10.5 g of vitamin mixture, 3.1 g of L-cystine, 2.1 g of choline bitartrate By mixing, a diet consisting of high fat diet + leek lyophilisate + formulation sulfur was prepared (AS of Table 3).

The feed was prepared by Feedlab Co., Ltd. (Hanam City, Gyeonggi-do).

1-6. Preparation of Fermented Leek Juice Lyophilized and Containing Sulfur

Using the same method as in the above 1-5, using a lyophilisate prepared in 1-2 above was prepared a feed consisting of high fat diet + fermented leek juice lyophilisate + formulation sulfur (FAS in Table 3).

The feed was prepared by Feedlab Co., Ltd. (Hanam City, Gyeonggi-do).

1-7. Preparation of Dietary, High-Fat, and Legal Sulfur + High-Fat Diets

For comparison experiments, dietary diets (CON in Table 3), high-fat diets (HFD in Table 3), and high-fat diets (high-fat diet + sulfur in table 3, HS in Table 3) containing legal sulfur are shown in Table 3 below. It was prepared as follows.

The feed was prepared by Feedlab Co., Ltd. (Hanam City, Gyeonggi-do).

material group CON HFD HS AS FAS Casein (g) 200 233.1 233.1 209.6 209.6 Sugar (g) 100 201.4 201.4 181.1 181.1 Glucose (g) 132 116.5 116.5 104.8 104.8 Corn starch (g) 397.5 84.8 84.8 76.3 76.3 Cellulose (g) 50 58.3 56.8 52.4 52.4 Soybean oil (g) 70 29.1 29.1 26.2 26.2 Pork oil (g) 0 206.9 206.9 186.0 186.0 Legal sulfur (g) - 0.0 1.5 0.870 0.870 ATR (g) - 0.0 0.0 100.0 0.0 FATR (g) - 0.0 0.0 0.0 100.0 Mineral mixture (g) 35 52.4 52.4 47.2 47.2 Vitamin mixture (g) 10 11.7 11.7 10.5 10.5 L-Cystine (g) 3 3.5 3.5 3.1 3.1 Choline stannate (g) 2.5 2.33 2.33 2.1 2.1 Total (g) 1000.0 1000.0 1000.0 1000.0 1000.0 Sum (kcal) 4000.0 4661.0 4661.0 4661.0 4661.0

* ATR: leek juice lyophilisate, FATR: fermented leek juice lyophilisate, CON: general dietary feed, HFD: high-fat diet, HS: high-fat diet containing staple sulfur (high-fat diet + sulfur), AS : High-fat diet containing leek lyophilisate and legal sulfur (high fat diet + leek lyophilisate + legal sulfur), FAS: High-fat diet containing fermented leek juice and legal sulfur (high fat diet + Fermented leek juice lyophilized + legal sulfur)

1-8. Animal experiment

Five week-old Wister rat male rats were purchased (Central Experimental Animal, Seocho-gu, Seoul) and used as a basic diet for one week, and six animals were randomly divided into five groups for six weeks. Each of the feeds prepared in 1-5 to 1-7 were provided to one experimental group, but each was ingested 20g per day, and the negative water was freely ingested. Animal breeding room was kept temperature and humidity, and the contrast was adjusted every 12 hours.

1-9. Weight Measurement of Laboratory Animals

The weight of each experimental animal was measured using a balance (Adventurer AR2140, OHAUS crop, USA) at weekly intervals.

1-10. Feed intake and calorie intake calculation

The feed intake (g) was calculated by subtracting the remaining amount (g) from the previous day's feed (g) and the calorie intake was the product of the feed intake and the calorie of the feed.

1-11. Long-term weight measurement

The weights of the spleen, liver, kidney, peri-renal fat and peritesticular fat of each experimental animal were measured and weighed. The liver was stored at -80 ° C after extraction.

1-12. Serum biochemical test

After the experiment, blood was collected from the posterior vein of each animal, and each blood was centrifuged at 3,000 rpm for 15 minutes, and serum was separated and stored frozen at -20 ° C. Total cholesterol, triglyceride ), HDL, LDL, AI (Atherogenic Index), AST (Aspartate aminotransferase), ALT (Alanine aminotransferase), glucose (Glucose) concentrations were measured. AI (Atherogenic Index) was calculated as ((total-C)-(HDL-C)) / HDL-C. Serum biochemical test was conducted by Biotoxtech Co., Ltd.

1-13. Liver analysis

1-13-1. Total lipid measurement of liver

Total lipids of the liver were extracted using the method of Folch et al.

50 mg of liver was collected and placed in a homogenizer (HS-30E, Daehan Science, Korea) and homogenized by adding 3 ml of distilled water, followed by 7 ml of chloroform. After centrifugation, the supernatant and suspended solids were removed, concentrated with N2 gas, and weighed to determine total lipid.

1-13-2. Determination of Total Cholesterol in the Liver

The total cholesterol of the liver was measured by dissolving 2 ml of ethanol in the sample concentrated with N2 gas in 1-13-1 and using a kit reagent (Asan Pharmaceutical, Korea).

3 ml of enzyme solution was added to 20 µl of the ethanol-added sample, and the absorbance was measured at 500 nm after standing at 37 ° C. for 5 minutes.

1-13-3. Triglyceride measurement of liver

500 μl of the ethanol-added sample in 1-13-2 was placed in an Eppendorf tube, ethanol was removed, and 50 μl of a solution of Triton X-100 and chloroform in a 1: 1 ratio was added. After adding 450 µl of chloroform and mixing the mixture, 3 ml of the enzyme reagent of the kit reagent was added to 20 µl of the mixture, and the resultant was left at 37 ° C. for 15 minutes, and the absorbance was measured at 550 nm to measure neutral fat.

1-14. statistics

All statistics were analyzed using SAS (Statistic Analytical Institute Cary NC, 1988), and the significance test between the means (p <0.05) compared the significant difference at the level of 95% of Tukey's Multiple Range Test.

1-15. result

1-15-1. Weight gain and feed intake

Table 4 shows the results of measuring body weight change and total feed intake for 6 weeks in each experimental group. Initial body weight was constant at 181g, but after 6 weeks body weight gain decreased significantly (p <0.05) in AS group compared to HFD group. There was no significant difference in total feed intake, but it was low in the HS group.

Initial weight Final weight Weight gain Daily Feed Intake CON 181.2 ± 5.88 ^NS 345.5 ± 7.29 ^bc 164.3 ± 1.41 ^b 20.8 ± 0.53 ^NS HFD 181.3 ± 6.47 367.0 ± 8.83 ^a 185.7 ± 2.36 ^a 19.6 ± 0.53 HS 181.0 ± 5.69 361.3 ± 6.25 ^ab 180.3 ± 0.56 ^a 19.0 ± 0.57 AS 181.0 ± 5.55 342.5 ± 11.83 ^c 161.5 ± 6.28 ^b 19.7 ± 0.37 FAS 181.3 ± 6.44 369.5 ± 14.01 ^a 188.2 ± 7.57 ^a 20.0 ± 0.35

* a, b, c: If the alphabet is different, it means that there is a significant difference (p <0.05).

* All values are expressed as mean ± standard deviation (n = 5).

1-15-2. Long-term weighing

The weight measurement results of liver, kidney, spleen, peritesticular fat and perirenal fat of each experimental group are shown in Table 5, FIGS. 1 and 2.

There was no significant difference in the weight of the liver, and in the height, the FAS group was significantly higher (p <0.05). The spleen was significantly higher (p <0.05) in the FAS group than in the CON and HFD groups.

In the testicular region, there was no significant difference in the HS and FAS groups compared to the HFD group, but it was significantly lower in the AS group (p <0.05). Fat weight was significantly lower (p <0.05).

liver kidney spleen CON 8.4 ± 0.52 ^NS 2.0 ± 0.08 ^b 0.5 ± 0.03 ^bc HFD 8.8 ± 0.74 2.0 ± 0.07 ^b 0.6 ± 0.06 ^bc HS 8.8 ± 0.45 2.1 ± 0.10 ^b 0.5 ± 0.05 ^c AS 8.4 ± 0.58 2.1 ± 0.07 ^b 0.6 ± 0.02 ^ab FAS 9.2 ± 0.17 2.3 ± 0.13 ^a 0.7 ± 0.05 ^a

* a, b, c: If the alphabet is different, it means that there is a significant difference (p <0.05).

* All values are expressed as mean ± standard deviation (n = 5).

1-15-3. Serum biochemistry

Serum biochemical test results of each experimental group are shown in Tables 6 and 7.

In ALT, there was no significant difference in all groups, but the results of AST were significantly lower in the HS, AS, and FAS groups (p <0.05) than in HFD, and significantly (p <) in the FAS group. 0.05) showed the lowest value. There was no significant difference in all groups, and there was a significant (p <0.05) difference between the HS and AS groups. The results of LDL-C showed no significant difference in the treated group compared to the HFD group, but decreased in both the AS and FAS groups. The AI result showed the lowest value in the AS group.

ALT (U / T) AST (U / T) AST / ALT ratio Glu (mg / dL) CON 55.3 ± 6.00 ^NS 187.4 ± 11.17 ^ab 3.1 ± 0.21 107.4 ± 6.23 ^ab HFD 69.0 ± 6.10 201.0 ± 7.84 ^a 3.3 ± 0.16 116.6 ± 13.87 ^ab HS 60.5 ± 5.60 167.7 ± 17.16 ^bc 2.7 ± 0.33 110.0 ± 1.83 ^b AS 69.0 ± 2.10 146.6 ± 7.29 ^cd 2.5 ± 0.47 134.5 ± 17.60 ^a FAS 48.2 ± 4.26 129.6 ± 16.84 ^d 2.3 ± 0.45 134.5 ± 17.60 ^ab

* a, b, c, d: If the alphabet is different, it means that there is a significant difference (p <0.05).

* All values are expressed as mean ± standard deviation (n = 5).

T-chol (mg / dL) TG (mg / dL) HDL-C (mg / dL) LDL-C (mg / dL) AI CON 58.4 ± 4.2 ^b 41.3 ± 2.87 ^c 17.5 ± 1.23 ^c 3.8 ± 0.17 ^a 2.34 HFD 66.4 ± 5.03 ^b 58.4 ± 10.41 ^bc 19.8 ± 1.05 ^c 3.3 ± 0.25 ^ab 2.36 HS 65.40 ± 5.50 ^b 79.0 ± 14.02 ^a 20.0 ± 1.58 ^bc 3.4 ± 0.21 ^ab 2.27 AS 64.5 ± 4.80 ^b 61.3 ± 4.04 ^ab 22.8 ± 1.81 ^ab 3.0 ± 0.64 ^b 1.83 FAS 76.5 ± 3.42 ^a 68.2 ± 5.87 ^ab 24.7 ± 1.61 ^a 2.7 ± 0.45 ^b 2.10

* a, b, c, d: If the alphabet is different, it means that there is a significant difference (p <0.05).

* All values are expressed as mean ± standard deviation (n = 5).

1-15-4. Total lipid measurement of liver

The result of measuring the total lipid of the liver of each experimental group is shown in FIG.

The amount of total lipid was significantly lower (p <0.05) in all groups than in the HFD group, and the lowest in the AS group.

1-15-5. Determination of Total Cholesterol in the Liver

The result of measuring total cholesterol of each experimental group is shown in FIG.

The AS and FAS groups showed significantly lower values than the HFD group (p <0.05).

1-15-6. Triglyceride measurement of liver

The result of measuring the liver triglyceride of each experimental group is shown in FIG.

There was no significant difference in the HS group compared to the HFD group, but the AS and FAS groups showed significantly lower values (p <0.05).

Claims (5)

A composition for preventing and improving arteriosclerosis comprising a fermented leek juice lyophilisate prepared by fermenting leek juice with Leuconostoc mensenteriodes and lyophilized and sulfur. delete The method of claim 1, wherein the composition
Atherosclerosis prevention and improvement composition, characterized in that to increase the concentration of HDL-cholesterol in the serum, and lower the concentration of LDL-cholesterol. Atherosclerosis prevention and improvement health functional food comprising the composition of claim 1 or claim 3. Functional livestock feed for preventing and improving atherosclerosis comprising the composition of claim 1 or 3.

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