KR101170348B1 - New rice variety 'jinsang' with improved eating quality of semidwarf type - Google Patents
New rice variety 'jinsang' with improved eating quality of semidwarf type Download PDFInfo
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Abstract
Description
본 발명은 단간형의 식미 특성이 개선된 벼 신품종 변종식물에 관한 것으로, 더욱 상세하게는 유메추쿠시 품종(Oryza sativa L.)을 모본으로 하고 밀키퀸 품종(Oryza sativa L.)을 부본으로 인공교배하여 얻어진 수정란에 N-methyl-N-nitrosourea를 돌연변이 유기물질로 처리한 다음, 수확된 종자를 전개하여 형성된 F1에서 단간이면서 돌연변이 유기물질 처리가 확인된 40% 이상 불임특성을 보이며 저아밀로스 특성을 지닌 개체주를 수확?선발하고, F2 이후 단간이면서 임성이 양호하고 아밀로스 함량이 낮은 특성을 지닌 계통을 선발하는 과정을 통해 육성되어 간장이 평균 75.7㎝로서 아밀로스 함량이 11.9% 수준이고, 도요 식미값이 76 수준으로 우수한 식미 특성이 균일하게 안정적으로 유지되는 벼 신품종 변종식물인 것에 관한 것이다.The present invention relates to a new plant variety of rice varieties with improved taste characteristics of the simple liver, more specifically Yumechukushi varieties ( Oryza sativa L.) and milky queen varieties ( Oryza) sativa L.) in the counterpart treated with N-methyl-N-nitrosourea the embryos obtained by mating with artificial mutations organic material and then, the inter-stage while the mutant organic material treated in the F 1 formed by developing the harvested seeds make 40% Harvesting and selecting individual strains with abnormal amyloid characteristics and low amylose characteristics, and fostering by selecting the strains with short fertility, good fertility and low amylose content after F 2 , soy sauce averages 75.7㎝. It is related to the new varieties of rice varieties, the content of which is 11.9% level, and the food taste value of 76, which is uniformly stable.
벼의 육종목표는 다수성, 안정성 및 양질성 등으로 대별할 수 있는데, 이러한 목표의 우선 순위는 사회와 경제적 여건 변화에 따라 변화되어 왔다. 경제발전과 함께 소득수준이 향상되면서 수량보다는 품질에 대한 소비자의 요구가 높아지게 되었다. 최근에는 국민 식생활의 변화에 따라 1인당 쌀 소비량도 줄어들고 연간 쌀 생산량이 수요량을 초과하는 현상이 지속되어 양질미를 선호하는 경향이 더욱 뚜렷해지고 있다. Rice breeding targets can be roughly classified into multiplicity, stability, and quality. The priorities of these targets have changed according to changes in social and economic conditions. As income levels have risen with economic development, consumers' demands for quality rather than quantity have increased. Recently, as the national diet changes, the consumption of rice per capita decreases and the annual rice production exceeds the demand.
밥쌀의 품질요소에는 외관특성, 이화학적 특성, 영양가, 기능성, 식미, 안전성 및 상품성 등이 있으나, 고품질 시대를 맞아 무엇보다도 가장 중요시되는 품질요소는 식미이다. 쌀의 식미는 품종, 기상 및 토양조건, 작기, 시비량, 수확시기 등의 재배기술과 건조, 저장, 도정 등의 수확 후 관리기술의 영향을 크게 받지만 품종이 갖는 특성의 비중이 가장 크다.The quality factors of rice include appearance, physicochemical properties, nutritional value, functionality, taste, safety and commerciality, but the most important quality factor in the high quality era is food taste. The taste of rice is greatly influenced by the cultivation techniques such as varieties, weather and soil conditions, small size, fertilization amount, harvest time and post-harvest management techniques such as drying, storage and milling, but the characteristics of varieties are the largest.
육종적으로 쌀의 품질과 식미를 개량하기 위해서는 우리나라의 소비자들이 선호하는 각종 식미 관련 특성을 가진 유전 자원을 찾아서 교배친으로 사용해야 한다. 그리고 새로 육성하는 고품질?양식미 품종은 그 동안 우리나라에서 육성한 양질미 품종이나 우리와 경쟁관계에 있는 외국의 양질미 품종과 유전적으로 차별화 시키는 것이 유리하다.In order to improve the quality and taste of rice by breeding, it is necessary to find and use genetic resources with various food-related characteristics that Korean consumers prefer. In addition, it is advantageous to genetically differentiate high-quality and cultured rice varieties that are newly cultivated from high-quality rice varieties that have been raised in Korea and foreign quality rice varieties that compete with us.
쌀의 탄수화물은 전분의 형태로 배유에 축적되어 있으며, 전분의 물리화학적 특성 및 쌀의 성분 분석을 통해 간접적으로 식미를 평가할 수 있다. 이에 관여하는 식미관련 특성에는 알카리붕괴도, 아밀로스 함량, 아밀로그램 특성, 단백질 함량 등이 주요한 인자로 작용한다. 쌀밥의 식미 평가를 객관적으로 신속하게 수행하기 위하여 여러 종류의 식미계가 개발되어 이용되고 있다. 그 중 Toyo-미도 메타 식미계는 백미를 단시간에 취반한 반증미판에 전자파를 조사하여 반사되는 밥의 윤기로부터 식미치를 제시해 주며, 실제 식미관능평가치와 80% 이상의 적중률을 나타내어 식미의 객관적 평가에 유용하게 이용되고 있다.Carbohydrates in rice are accumulated in the milk in the form of starch, and the taste can be indirectly evaluated by analyzing the physical and chemical properties of the starch and the composition of the rice. In relation to the food-related properties involved in this, the main factors such as alkali disintegration degree, amylose content, amylogram properties, protein content. In order to quickly and objectively evaluate the taste of rice, various types of food systems have been developed and used. Among them, the Toyo-Mido meta-flavor system presents the taste value from the radiance of rice reflected by reflecting electromagnetic waves on a semi-enhanced plate that cooks white rice for a short time, and shows the actual food taste evaluation value and more than 80% hit rate for objective evaluation of food taste. It is usefully used.
일반적으로 자포니카 품종에서 식미가 양호한 품종들의 특성은 도요 식미치가 높고 단백질함량이 낮으며, 호화온도가 낮고 최고점도와 강하점도는 높으며, 최종점도 및 취반점도가 낮은 것으로 알려져 있다.In general, saponica varieties are known to have a good taste and have a high Toyo taste, low protein content, low gelatinization temperature, high peak and drop viscosity, and low final and cooking viscosity.
최근 우리나라에서 도입품종의 양식미 품종으로 고시히까리, 저아밀로스 품종인 밀키퀸 품종의 재배 및 가공 판매가 증가하고 있다. 그러나 이들 벼 품종들은 장간으로 도복에 약한 등 농가에서 재배상 관리가 어려운 난점이 있었다. Recently, cultivation and processing sales of Milky Queen varieties of Goshihikari and low amylose varieties have been increasing in cultivated and cultivated cultivars introduced in Korea. However, these rice varieties were difficult to manage in the farms, such as weak in dormitory due to jangjang.
따라서 이러한 현행 양식미의 문제점을 육종적으로 해결하여 외국의 양질미 품종과 유전적으로 차별화되고 소비자들의 기호도를 충족시킬 수 있는 고품질 신품종의 개발이 시급히 요구되고 있다.Therefore, it is urgently needed to develop high quality new varieties that are genetically differentiated from foreign high quality rice varieties and satisfy consumers' tastes by breeding these current farming problems.
상기와 같은 요구에 따라 안출된 본 발명은 유메추쿠시 품종과 밀키퀸 품종을 인공교배하여 얻어진 수정란에 돌연변이를 처리하고 일련의 계통선발과정을 통해 육성되어 후대에 걸쳐 우수한 특성이 안정적으로 유지되는 아밀로스 함량이 낮고, 식미특성이 개선된 벼 신품종 식물을 제공하는데 그 목적이 있다.The present invention devised in accordance with the requirements as described above is processed through mutations in the fertilized eggs obtained by artificially crossing the yumechukushi varieties and milky queen varieties and fostered through a series of line selection process amylose stable excellent properties for future generations Its purpose is to provide a new variety of rice plants with low content and improved taste.
위와 같은 목적을 달성하기 위한 본 발명은 유메추쿠시 품종(Oryza sativa L.)을 모본으로 하고 밀키퀸 품종(Oryza sativa L.)을 부본으로 인공교배하여 얻어진 수정란에 N- methyl -N- nitrosourea를 돌연변이 유기물질로 처리한 다음 수확된 종자를 전개하여 형성된 F1에서 단간이면서 돌연변이 유기물질 처리가 확인된 40% 이상 불임특성을 지닌 개체주를 선발하고, F2 이후 단간이면서 임성이 양호하고 아밀로스 함량이 낮은 특성을 지닌 계통을 선발하는 과정을 통해 육성되어 간장이 평균 75.7㎝로서 아밀로스 함량이 11.9% 수준이고, 도요 식미값이 76 수준으로 우수한 식미 특성이 균일하고 안정적으로 유지되는 벼 신품종 변종식물(수탁번호: KCTC 11984BP)인 것에 특징이 있다.The present invention for achieving the above object is Yumechukushi variety ( Oryza sativa L.) and milky queen varieties ( Oryza) 40% or more of the short-term and mutant-organic substance was confirmed in F 1 formed by treating N- methyl - N - nitrosourea with mutant organic material in the fertilized eggs obtained by artificial cross - culture with sativa L.). The infertility strains were selected, and after F 2 , the strains were grown through the selection of strains with good fertility and low amylose content, soy sauce averaged 75.7cm and amylose content was 11.9%. It is characterized by the new varieties of rice plants (Accession No .: KCTC 11984BP), whose food value is 76, with excellent food properties being uniform and stable.
본 발명의 벼 신품종 변종식물은 배유의 투명도가 5로서 중간정도의 불투명 배유 특성을 지닌 것을 특징으로 한다. New varieties of rice plants of the present invention is characterized by having a opacity of the medium opacity as the transparency of the endosperm 5.
또한, 본 발명의 벼 신품종 변종식물은 현미의 길이가 평균 4.86㎜, 폭이 평균 2.91㎜, 두께가 평균 2.04㎜ 수준으로 장폭비가 1.67 정도의 단원형 품종인 것을 특징으로 한다. In addition, the new varieties of rice plant varieties of the present invention is characterized in that the length of the length of about 1.86 mm, the average length of 2.86 mm, the average thickness of 2.91 mm, the thickness of about 1.67.
또한, 본 발명의 벼 신품종 변종식물은 조중생벼로서 현미 천립중이 평균 21.0g인 것을 특징으로 한다. In addition, the new varieties of plant varieties of the present invention is characterized in that the average weight of 21.0g of brown rice grains as crude raw rice.
본 발명에 따른 벼 신품종 변종식물은 도입 양식미인 밀키퀸, 고시히까리 등과는 달리 단간 초형으로 농가재배가 용이하고, 아밀로스 함량이 낮고 단백질 함량이 낮으며, 도요 식미값이 높은 등 식미 특성이 월등히 우수하여 소비자의 기호에 부응하는 고품질 벼 신품종인 것으로 평가되었으며, 후대에 걸쳐 우수한 유전적 형질이 균일하게 안정적으로 유지되어 벼 재배 농가의 소득증대에 크게 기여할 것으로 기대되었다.The new varieties of rice plants according to the present invention, unlike milky quince, Koshihikari, which are introduced cultured rice, is easy to cultivate farmhouses, and has excellent amyloid traits such as low amylose content, low protein content, and high food taste. It was evaluated as a new high-quality rice variety that meets consumers' preferences, and it is expected that excellent genetic traits will be maintained uniformly and stably for future generations, which will greatly contribute to income growth of rice farmers.
도 1은 본 발명에 따른 벼 신품종 변종식물의 육성계통도이다.
도 2는 본 발명에 따른 벼 신품종 변종식물의 육성계보도이다.
도 3은 본 발명에 따른 벼 신품종 변종식물의 식물체 사진이다.
도 4는 본 발명에 따른 벼 신품종 변종식물의 정조 및 현미 사진이다.
도 5는 본 발명에 따른 벼 신품종 변종식물의 DNA 단편 사진이다.1 is a growing system of the new varieties of rice plants according to the present invention.
Figure 2 is a breeding lineage of new varieties of rice plants according to the present invention.
Figure 3 is a plant picture of a new varieties of rice plants according to the present invention.
Figure 4 is a picture of jeongjeong and brown rice of a new variety of rice plants according to the present invention.
5 is a DNA fragment picture of a new varieties of rice plants according to the present invention.
이하, 본 발명에 따른 벼 신품종 변종식물의 육성과정 등에 대하여 실시 예를 통해 보다 구체적으로 설명한다.
Hereinafter, the growing process of the new varieties of rice varieties according to the present invention will be described in more detail through examples.
실시예Example 1 : 신품종 '진상'벼의 육성과정 1: Cultivation Process of New Varieties of 'Truth'
지역특화 고브랜드 양식미 품종 육성을 위해 양식미 특성의 단간인 Yumetsukushi(유메추쿠시)를 교배모본으로 선정하고, 가공성 및 식미가 우수한 품종으로 밀키퀸(Milky queen)을 교배부본으로 선정하여 2000년 하계에 인공교배 후 수정란에 MNU (N- methyl -N- nitrosourea)라는 돌연변이 유기물질을 처리한 후, 수확된 종자를 JCH33M으로 표기하고, 2001년 F1 종자를 파종하여 동년 수확된 개체에서 단간이면서 돌연변이 유기물질 처리가 확인된 40% 이상 불임특성을 갖으며 배유의 특성에서 투명립 메벼와 불투명의 저아밀로스 미립과 분리하는 개체에서 저아밀로스 특성의 미립을 선발했다. 2002년 F2 개체에서 임성, 재배특성이 양호하고 저아밀로스 특성의 보이는 단간인 개체를 선발하였고, 2003년부터 2008년까지 매년 포장에서 계통재배하면서 단간 특성을 갖고, 임성, 초형이 양호하며, 아밀로스 함량이 낮은 JCH33M-15-1-9-1-5-1-1-1의 계통을 선발, 고정하였다. 선발된 계통(JCH33M-15-1-9-1-5-1-1-1)에 대해 HJ-2라고 가칭하고, 2009년~2010년에 난괴법 3반복으로 생산력검정 및 농가실증시험을 수행하여 균일성과 재배 안정성을 확인하여 진상벼라고 명명하였다(수탁번호: KCTC 11984BP). 품종의 육성과정과 육성계보에 대해 <도 1>과 <도 2>에 각각 도식하였다. 한편, 상기 육성과정의 재배조건은 농촌진흥청 '벼 표준재배법'에 준하였으며, 2010년에 실시한 포장 시험 조건은 다음과 같았다.In order to nurture local specialty high-brand cultured rice varieties, Yumetsukushi, which is a cultivated variety, was selected as a breeding model, and Milky queen was selected as a breeding breed in 2000. after treating the organic material mutation of MNU (N- methyl -N- nitrosourea) in embryos after artificial crossing in the summer, the representation of harvested seed in JCH33M, and the inter-stage in the same year, yet harvested object by inoculating 2001 F 1 seed More than 40% of the mutant organic material was confirmed to have infertility characteristics, and in the characteristics of endosperm, it was selected as a low-amylose particle from the individual separated from the opaque low-amylose grain. In 2002, F 2 objects fertility, cultivation characteristics are good, and was starting to look interstage the object of a low amylose characteristics, from 2003 to 2008, while strains grown in packaging every year has the interstage characteristics, fertility, and sheath type is good, amylose A strain of JCH33M-15-1-9-1-5-1-1-1 with low content was selected and fixed. The selected strain (JCH33M-15-1-9-1-5-1-1-1) was designated as HJ-2, and the productivity test and the farmhouse demonstration test were conducted in 2009-2010 with 3 repetition of the lump mass method. Was confirmed as uniformity and cultivation stability was named Jinsang rice (Accession Number: KCTC 11984BP). The breeding process and breeding lineage of the varieties are shown in <Fig. 1> and <Fig. 2>, respectively. On the other hand, the cultivation conditions of the development process was in accordance with the Rural Development Administration 'standard rice cultivation method', and the field test conditions conducted in 2010 were as follows.
○ 시험지 위치 및 특성 : 수원시 권선구 호매실동 답 7-2, 34-2번지, 지형(중서부평야)○ Location and Characteristics of Test Site: 7-7, 34-2, Homaesil-dong, Gwonseon-gu, Suwon-si, Topography (Middlewest Plain)
○ 파종기 및 이앙기 : 2010년 4월 22일 파종, 2010년 5월 21일 이앙○ Planting period and rice transplanter: Sowing on April 22, 2010, Yiang on May 21, 2010
○ 시험구 면적 및 반복수 : 약 10평 / 3반복○ Test area and number of repetitions: about 10 pyeong / 3 repetitions
○ 재식거리 : 30cm x 15cm○ Planting Distance: 30cm x 15cm
○ 토질 및 토성 : 식양토○ Soil and Saturn: Soil
○ 시비량 및 시비방법 : N-P-K = 11-6-6+4/10a 를 기비시용○ Fertilization amount and application method: N-P-K = 11-6-6 + 4 / 10a
N-K = 18-16 을 이삭거름으로 시용 Use N-K = 18-16 as ear manure
○ 시험구 표본채취 규모 : 1반복 당 37주로 3반복 수확○ Sampling size of test plot: 3 repetitive harvests with 37 weeks per repetition
○ 수확일자 : 2010년 9월 23일
○ Date of Harvest: September 23, 2010
실시예Example 2 : 신품종 '진상'벼의 주요특성 2: Main characteristics of new varieties 'Tangjin' rice
상기 실시예 1에서 육성한 '진상'벼의 주요 농업형질과 미질특성에 대해 표 1, 표 2, 표 3 및 도 4에서 대조품종인 '추청'벼와 비교하였다.
The main agricultural traits and quality characteristics of the 'grown' rice grown in Example 1 were compared with the 'chuchu' rice, which is a control variety in Tables 1, 2, 3 and 4.
상기 표 1에 나타낸 바와 같이, 본 발명에 따른 '진상'벼의 출수기는 대조품종인 추청보다 11일 빠른 조중생종이고, 간장은 75.2cm로 대조품종 추청의 84.9cm 보다 짧았으며, 현미 천립중은 20.91g 으로 대조품종의 20.17g 보다 약간 무거웠다. As shown in Table 1, the extract of the 'Jinjin' rice according to the present invention is a crude cultivar 11 days faster than the control varieties Chuchu, soy sauce is 75.2cm shorter than 84.9cm of the control varieties, and the brown rice is 20.91 g was slightly heavier than 20.17 g of the control variety.
또한 상기 표 2에 나타낸 '진상'벼의 곡실 특성은 현미길이가 4.86mm이고, 폭은 2.91mm이며, 두께는 2.04mm 수준으로 장폭비가 1.67 정도의 단원형 특성을 보였고, 정현비율은 대조품종과 비슷한 81.2% 수준이며, 수량성은 457kg/10a로 대조품종인 '추청'벼의 453kg/10a보다 약간 높았다. In addition, the grain characteristics of the 'Truth' rice shown in Table 2 are 4.86mm long, 2.91mm wide, 2.04mm thick, showing a long width ratio of about 1.67, and the sine ratio of The yield was similar to 81.2%, and the yield was 457kg / 10a, slightly higher than the 453kg / 10a of the Chuchu rice.
상기 표 3에 나타낸 본 발명에 따른 '진상'벼의 배유 투명도는 5로 대조품종 추청의 1보다 높은 중간의 찰벼 특성이고, 알카리붕괴도는 완전히 분해되며, 향취성이 없는 미질특성을 보였다. 특히 아밀로스함량은 11.9%로 대조품종 19.1%보다 낮았고, 도요 식미값은 대조품종인 '추청'벼 보다 높은 76 수준이었다.
The opacity of the 'Truth' rice according to the present invention shown in Table 3 is 5 times higher than the 1 of the control varieties Chukjang, alkaline decay degree is completely decomposed, and showed a tasteless taste without taste. In particular, the amylose content was 11.9%, lower than that of the control variety, 19.1%, and the food content of Toyo was 76, higher than that of the control product, Chuchu.
실시예Example 3 : 3: DNADNA 단편 분석 Fragment analysis
본 발명의 벼 신품종 변종식물에 대해 ABI3500 (Applied Biosystems, USA) 및 QIAXEL (QIAGEN, USA)를 이용하여 DNA profile을 분석한 결과, 4개의 마커를 이용할 경우 현재 육성된 주요 중간찰벼 특성을 갖는 만추벼, 백진주, 백진주 1호와 구분이 가능하고, 모본으로 사용된 밀키퀸의 특성과 유메추쿠시의 특성이 유전되었음을 알 수 있다. 즉 RM21, RM48, RM206의 3개 마커 조합 또는 RM48, RM206, RM246의 3개 마커 조합을 이용할 경우 유통 중간찰벼와 구분이 가능하고, 또한 본 발명의 벼 신품종 변종식물은 RM48에서 유메추쿠시의 단편을 RM206에서는 밀키퀸의 단편을 보여 모본 및 부본의 특성을 보였다 (표 4, 도 5 참조).
As a result of analyzing the DNA profile of ABI3500 (Applied Biosystems, USA) and QIAXEL (QIAGEN, USA) for the new varieties of rice plants of the present invention, the rice paddies having the main intermediate rice properties currently grown using four markers It can be distinguished from baek jin-ju and baek jin-ju No. 1, and it can be seen that the characteristics of milky quince and yumechukushi used as mothers are inherited. That is, when using three marker combinations of RM21, RM48, and RM206 or three marker combinations of RM48, RM206, and RM246, it is possible to distinguish them from intermediate rice in circulation. In RM206, fragments of milkyquines showed the characteristics of the parent and the minor (see Table 4, Figure 5).
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR101492491B1 (en) | 2013-07-29 | 2015-02-11 | 농업회사법인 주식회사시드피아 | Low amylose rice jinsang 2 |
| KR101536186B1 (en) * | 2015-03-30 | 2015-07-13 | 농업회사법인 주식회사시드피아 | New Glutinous Rice Variety Having Aroma |
| KR101960068B1 (en) * | 2018-10-16 | 2019-03-19 | 고려대학교 산학협력단 | Novel Variety of Extra-short Wheat cultivar 'Mini' |
| KR20200069121A (en) | 2018-12-06 | 2020-06-16 | 농업회사법인 주식회사시드피아 | New Rice Cheon su hyang Keun Nun Chal 1Se and Breeding Method of thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101492491B1 (en) | 2013-07-29 | 2015-02-11 | 농업회사법인 주식회사시드피아 | Low amylose rice jinsang 2 |
| KR101536186B1 (en) * | 2015-03-30 | 2015-07-13 | 농업회사법인 주식회사시드피아 | New Glutinous Rice Variety Having Aroma |
| KR101960068B1 (en) * | 2018-10-16 | 2019-03-19 | 고려대학교 산학협력단 | Novel Variety of Extra-short Wheat cultivar 'Mini' |
| KR20200069121A (en) | 2018-12-06 | 2020-06-16 | 농업회사법인 주식회사시드피아 | New Rice Cheon su hyang Keun Nun Chal 1Se and Breeding Method of thereof |
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