KR101168379B1 - Composition for Prevention and Treatment of Gout, Hypertension and Cancer comprising Acanthopanax senticosus Fruits Extracts - Google Patents

Abstract

The present invention relates to a composition for the prevention and treatment of gout, hypertension and cancer comprising prickly pear fruit extract, more specifically, the prickly pear fruit extract and red pigment separated therefrom have antioxidant activity and active oxygen scavenging ability, It shows that xanthine oxidase inhibitory activity and ACE (angiotensin converting enzyme) inhibitory activity, and cell growth inhibition effect on human cancer cell line.
According to the present invention can provide a composition for the prevention, treatment and improvement of gout, hypertension and cancer comprising prickly pear fruit extract.

Description

Composition for Prevention and Treatment of Gout, Hypertension and Cancer comprising Acanthopanax senticosus Fruits Extracts}

The present invention relates to a composition for the prevention and treatment of gout, hypertension and cancer comprising prickly pear fruit extract.

Oxidation by the active oxygen species (reactive oxygen species), such as hydrogen peroxide (H ₂ O ₂₎ ^- aging, cancer, adult diseases and chronic diseases is the hydroxyl radical (OH), superoxide radical (O ₂₎ generated in the living body It is known that it is caused by the metabolic by-products, and the antioxidant substances that remove these reactive oxygen species are widely distributed in animals and plants. In particular, medicinal crops contain vitamin C, vitamin E, carotenoids, etc., which exhibit strong antioxidant activity, as well as flavonoids such as flavone, isoflavone, anthocyanin, and catechin. It has a wide range of antioxidant mechanisms. This antioxidant activity has been demonstrated in in vivo and in vitro experiments.

Prickly Pear is a perennial shrub belonging to the genus Araliaceae Acanthopanax , better known as 'Siberian ginseng' in Russia and Europe, and has anti-fatigue, antistress, immune activity, antidepression, alleviation, anti-diabetes Various pharmacological effects such as cancer and cancer are recognized at home and abroad.

The herbal medicine is called `` Acanthopanacis Cortex '', which is used as a medicinal herb, bark or root bark. The stem and root bark are lignan glycosides eleutherosides A, B, C, D, E, It is reported that I, K, M and chlorogenic acid, sesamin, caffeic acid, and the like.

Polysaccharides isolated from organolpi have been reported to have an immunomodulatory effect, and polysaccharides from p. Ogalpi induce proliferation of total spleen cells in vitro and B- in vivo. It has been reported to strongly increase the antibody formation of B-cells.

Recently, the research of Thorn oak bark tree is diverse with rapid mass growth, physiological activity and active ingredient, and there have been reports on the physiological activity and supercritical extraction of natural components containing Thorn oak tree tree, but most of the studies on useful components have been used for bark tissue. The situation is limited to the study of separation and physiological activity of useful components of origin.

In other words, there have been no studies on the pharmacological effects of the extract of Prunus albagae fruit, and no studies on the composition for the prevention and treatment of gout, hypertension and cancer containing p.

The present invention was confirmed that the prickly berry extract exhibits excellent antioxidant, antigout, antihypertensive and anticancer activity. That is, the present invention was isolated and identified an anthocyanin compound, which is a red pigment isolated from the fruit extract of prickly pears (Cyanidin 3-sambubioside), the anthocyanin-based red pigment as an active ingredient It was confirmed by the TBA method that the extract contains antioxidant activity, has an active oxygen scavenging activity, xanthin oxidase inhibitory activity, ACE (angiotensin converting enzyme) inhibitory activity, and various human cancer cell lines It was confirmed that the cell growth inhibitory effect was shown, and found that it can be used as a composition for the prevention, treatment and improvement of gout, hypertension and cancer, and completed the present invention.

Therefore, the present invention is a composition for the prevention and treatment of gout, hypertension and cancer, and gout, hypertension and cancer containing a red pigment isolated from the extract of P. The purpose is to provide a composition for the prevention, treatment and improvement of.

As an example for achieving the above object, the present invention is characterized by a composition for the prevention and treatment of gout, hypertension and cancer containing prickly pear fruit extract as an active ingredient.

As another example for achieving the above object, the present invention is isolated from the Prickly Pear Extract, a composition for the prevention and treatment of gout, hypertension and cancer containing an anthocyanin-based red pigment represented by the following formula (1) as an active ingredient Characterized by;

As another example for achieving the above object, the present invention is characterized by a health food for the prevention and improvement of gout, hypertension and cancer containing prickly galli fruit extract as an active ingredient.

As another example for achieving the above object, the present invention is isolated from the Prickly Pear Extract, health for preventing and improving gout, hypertension and cancer containing an anthocyanin-based red pigment represented by the formula (1) as an active ingredient Characterized by food.

Hereinafter, the present invention will be described in detail.

The method of extracting and separating the red pigment from the thorny red pepper fruit of the present invention is as follows.

First, prickly lychee fruit is dried and pulverized at room temperature (below 30 ° C.) for at least 24 hours to form a dry powder, which is immersed in water, alcohol or alcohol solution, extracted at room temperature, and then filtered and removed insoluble matter. Get At this time, the thorny red pepper fruit extract is a process of extracting by repeatedly performing the process of immersing for 24 to 72 hours by adding the dry thorny red pepper fruit powder to water, alcohol or alcohol aqueous solution at room temperature for 2 to 4 times, immersion The temperature is to be made at room temperature specifically below 30 ℃ as room temperature. When the immersion temperature is higher than 30 ℃, the yield of prickly pear fruit extract is high, but the stability of the anthocyanin compound which is the active ingredient contained in the prickly pear fruit extract cannot be guaranteed.

The alcohol may be a lower alcohol having 1 to 4 carbon atoms, the alcohol concentration of the aqueous alcohol solution used for immersion is preferably in the range of 20 to 80%.

When the immersion time is short, it is difficult to completely extract the anthocyanin-based compounds in the Prunus vulgaris extract, and when the immersion time is long or the number of repetitions is more than four times, the efficiency tends to be lowered.

The prickly pear extract is concentrated by removing the solvent, concentrating and performing column chromatography to obtain anthocyanin-based pigment fractions, each fraction is concentrated to dryness and redissolved, and then purified by preparative HPLC.

The structure of the obtained anthocyanin compound was identified by various spectroscopic methods such as NMR, MS, etc. As a result, an anthocyanin-based red pigment having the structure shown in Chemical Formula 1 was obtained.

As described above, the anthocyanin-based red pigment extracted from the fruit of P. falciparum was measured by TBA method using lipid peroxidation system using Feton's reagent, and the antioxidant activity was confirmed, and the free radical scavenging activity measuring method using DPPH It confirmed the active oxygen scavenging ability, and showed an inhibitory activity against xanthin oxidase, which catalyzes the formation of uric acid in the body, and ACE (angiotensin converting), which catalyzes the conversion to angiotensin II, which acts as a potent blood pressure raising factor. inhibitory activity against enzymes, and no effect on normal cells, but showed cell growth inhibition effect on various human cancer cell lines, and for the prevention, treatment and improvement of gout, hypertension and cancer containing it as an active ingredient. It can be used as a composition.

The cancer may include, but is not limited to, prostate cancer, colon cancer, gastric cancer, lung cancer, blood cancer, and various solid cancers.

On the other hand, the composition for the prevention and treatment of gout, hypertension and cancer comprising the prickly pear fruit extract or anthocyanin-based red pigment of Formula 1 as an active ingredient is administered orally or parenterally during clinical administration, for example, intravenous, subcutaneous It can be applied intraperitoneally or topically.

The compositions may be formulated for oral administration such as tablets, troches, lozenges, aqueous or oily suspensions, prepared powders or granules, emulsions, hard or soft capsules, syrups or elixirs. Is formulated. Binders such as lactose, saccharose, sorbitol, mannitol, starch, amylopectin, cellulose or gelatin for formulation into tablets and capsules; Excipients such as dicalcium phosphate; Disintegrants such as corn starch or sweet potato starch; Lubricants such as magnesium stearate, calcium stearate, sodium stearyl fumarate or polyethylene glycol wax. In the case of a capsule formulation, in addition to the above-mentioned substances, a liquid carrier such as fatty oil is contained.

In addition, the composition of the present invention can be administered parenterally, parenteral administration is by subcutaneous injection, intravenous injection, intramuscular injection, intrathoracic injection injection method. To formulate into a parenteral formulation, the composition may be mixed in water with stabilizers or buffers to prepare a solution and formulated in unit dosage forms of ampoules or vials.

The effective dosage of the prickly pear fruit extract or the compound of formula 1 of the present invention may vary according to the age, physical condition, weight, etc. of the patient, but is generally 1 to 50 mg / day per kg of adult patient, preferably Is 5 to 20 mg / day, and may be divided into several times a day, preferably two to three times a day at regular time intervals according to the judgment of a doctor or a pharmacist.

In addition, the present invention has been identified and separated from the thorny golgalpi fruit extract or anthocyanin-based red pigment represented by the formula (1) because it is stable to the human body suitable for use in food, gout, hypertension and cancer It can be developed as a health food having a preventive and improving effect.

0.01 to 50% (w / w) of the total weight, preferably 1 to 30% (w / w) of the total weight when the pangiogalpi fruit extract of the present invention or the anthocyanin-based red pigment represented by Formula 1 is included in the health food It can be used as a range.

That is, various foods can be prepared by conventionally known methods by adding them in conventional palates such as noodles, tofu, cereals, breads, chewing gum, candy, confectionary, such as ramen noodles, raw noodles, etc. It may also be formulated into a general formulation such as tablets, granules, pills, hard capsules, soft capsules or liquid formulations, may be prepared in a juice, pouch, beverage, or a variety of ingredients, other ingredients Depending on the formulation, those skilled in the art can appropriately select and combine.

The present invention is an extract of P. falciparum fruit, that is, the extract containing an anthocyanin-based red pigment represented by the formula (1) as an active ingredient, antioxidant activity such as active oxygen scavenging ability, xanthin oxidase inhibitory activity, ACE (angiotensin converting) By confirming that the enzyme) inhibitory activity and cell growth inhibition effect on the human cancer cell line can be expected, it can be expected that the natural extracts of the fruit extracts of the natural thorns can be used as a composition for the prevention, treatment and improvement of gout, hypertension and cancer. .

Figure 1 shows the antioxidant activity of Acanthopanax senticosus Fruits Extracts (ASFE).
Figure 2 shows the HPLC chromatogram of Prickly Pear Extract.
Figure 3 shows the HPLC chromatogram of the pure compound isolated from the Prunus golpi fruit extract.
Figure 4 shows the chromatogram of the UV-VIS absorbance characteristics of the pure compound isolated from P.
Figure 5 shows the results of LC-ESI / MS analysis of the main components containing pure isolated prickly pear fruit.
Figure 6 shows the results of ¹ H-NMR analysis of the main components containing pure isolated prickly pear fruit.
Figure 7 shows the results of ¹ H, ¹³ C hetro 2D-NMR analysis of the main components containing pure isolated prickly pear.

Hereinafter, the present invention will be described in detail with reference to examples, but the present invention is not limited by the following examples.

Example  1. Preparation of Prickly Pear Extract

Prickly fruit was dried and pulverized at 30 ° C. for more than 24 hours, and then, 1,000 g of 100% dry powder was added, followed by extraction at room temperature for 24 hours, followed by filtration. The residue was extracted three times.

The extract was evaporated and ethanol at 35 ℃ vacuum concentration device, passed by in an aqueous solution passed through a sep-pak C ₁₈ Cartridge solution was discarded, and by recovering the components adsorbed on sep-pak C ₁₈ Cartridge with ethanol Acanthopanax senticosus fruit An extract was prepared.

Example  2. Determination of Antioxidant and Reactive Oxygen Scavenging Activity of Prickly Pear Extract

As for the antioxidant activity assay of P. falcipar fruit extract, a lipid peroxidation system using Feton's reagent was used.

Prickly Pear Extract Extracted in Example 1 was prepared for each concentration of 10, 50, 100, 500 μg / mL, 2% sodium dodecyl sulphate, 1 μM perus chloride And 0.1 mL of concentration and 0.1 mL of ethyl linoleate (0.1 μL) were added to an incubation media containing 0.5 mM hydrogen peroxide. After the incubation media was left at 55 ° C. for 16 hours, 0.2 mL of each reaction solution was taken into a new tube and 50 μL of 4% BHT was added to prevent further oxidation. As a control of antioxidant activity evaluation, α-tocopherol was used. Antioxidant activity assay was evaluated by TBA (thiobarbituric acid assay) method, the absorbance was calculated by measuring the absorbance at 535 nm, the results are shown in Figure 1.

As shown in FIG. 1, the concentration of the extract was measured using a lipid peroxidation system using a Feton's reagent to assay the antioxidant activity of the P. falcipar fruit extract. Showed a marked antioxidant activity from, and the treatment of 50 ㎍ / mL showed a more than threefold increase in activity compared to the 10 ㎍ / mL treatment group, and the antioxidant activity was higher than that of α-tocopherol at all concentrations. Activity was shown [FIG. 1].

Free radical scavenging activity was measured using free radical DPPH (1,1-diphenyl-2-picryl hydrazyl). That is, 4 mL of methanol was added to the test tube, and the sample compound was added according to concentrations (1,000 ppm 1.5 to 30 μL), and then 1 mL of the 0.15 mM DPPH solution was added thereto to react at room temperature for 30 minutes, and the absorbance was measured at 520 nm. At this time, initial (Ai), blank (Ab) without substrate and DPPH were measured, and absorbance (As) was measured after sample addition. RC ₅₀ (㎍ / mL) was 50% of control group without compound. The concentration of the compound was decreased and compared with the existing antioxidants α-tocopherol and BHA, and the results are shown in Table 1 below. [Table 1. DPPH of Acanthopanax senticosus Fruits Extracts (ASFE) Free radical scavenging activity].

Sample DPPH radical scavenging activity
(RC ₅₀ μg / mL) ASFE 11.24 ± 0.53 α-tocopherol 12 BHT 14 Mean ± S.E obtained from six experiments

As shown in Table 1, as a result of measuring free radical scavenging activity of P. falcipar fruit extract, it showed an antioxidant activity (IC ₅₀ ) of 11.24 µg / mL, indicating a scavenging activity similar to that of the control reagent, α-tocopherol or BHT.

Example  3. Xanthine of Prickly Pear Extract Oxidase  Inhibition activity measurement

Generally, superoxide radicals are generated by Xanthine Oxidase in vitro. The superoxidative radical scavenging activity of P. falcipar fruit extract was assayed using nitro-blue tetrazolium (NBT) reduction method. In other words, 10, 50, 100, 10, 50, 100, 100, 500, 100, 100, in 0.1 mM phosphate buffer (pH 8.0) containing 0.8 mM xanthine and 0.1 mM phosphate buffer (pH 8.0) containing 0.48 mM NBT Each mixture was treated at a concentration of 500 μg / mL to make a mixture, and the mixture was incubated at 37 ° C. for 5 minutes, and 1.0 mL of XOD (0.049 U / mL) was added to start the reaction, followed by 20 minutes at 37 ° C. Reacted. 2.0 mL of 69 mM SDS was then added to terminate the reaction and the absorbance was measured at 560 nm. The concentration of the compound (IC ₅₀ , 50% inhibition concentration) was shown to reduce the inhibitory effect on NBT reduction by 50% according to the amount of sample added by concentration, and compared with (+)-catechin as a control. Table 2 shows the following [Table 2. Xanthine oxidase inhibitory effect of prickly berry extract (ASRE)].

Superoxide radicals act as the most important radicals among the active oxygen generated in living cells, and these radicals affect the peroxidation of unsaturated fatty acids. The excess oxidative radicals generated in cells can be measured using a xanthine oxidase (XO) assay, which catalyzes the oxidation of xanthine into uric acid. Thus the scavenging activity of the superoxidant radical is expressed as one of the most important ways of describing the antioxidant activity mechanism.

Xanthine oxidase is an enzyme that produces superoxide radicals in the process of producing uric acid with xanthine as a substrate. Xanthine oxidase, one of the free radical generation systems in vivo, is a nonspecific enzyme involved in metabolism such as purine, pyrimidine pteridine aldehyde, and heterocyclic compound. Mainly catalyzes the reaction of oxidizing xanthine to uric acid and hypoxanthine, a metabolite of the purine body.

Gout is a long-lasting high concentration of uric acid in the blood, forming crystals of uric acid, which deposits in various tissues and causes various symptoms. Gout patients accumulate uric acid in crystalline forms in many organs. It is a disease that causes complications in the kidneys and heart due to abnormal production and abnormal process of excretion into the kidneys. Currently, XO inhibitors have been used as a treatment for hyperuricemia causing gout, kidney stones, ischemia and cardiomyopathy, and there is an urgent need to develop inhibitors for the aging age.

Sample ASFE (+)-catechin Xanthine oxidase inhibition activity
(IC 50 μg / mL) 36.9 ± 3.4 7.2 ± 0.5 Mean ± S.E obtained from six experiments

As shown in Table 2, as a result of examining the inhibitory effect of XO activity of P. falcipar fruit extract, the concentration required to inhibit XO activity by 50% was about 36.9 ㎍ / mL, which did not reach the control active substance (+)-catechin. It can be seen that it exhibits a fairly high activity.

As a result of comparing and testing the inhibitory effect of XO activity (IC ₅₀ ) against 170 herbal medicines used in connection with gout, the outbreak extract of Junus effusus L. var. Decipiens Buchen was found to be 29.4, Sellaginella tamariscina. Spring) In comparison with the report that the outpost extract showed the highest activity at 33.7 ㎍ / mL, it is judged that P. falcipar fruit extract has an inhibitory effect on XO activity similar to pulpy or whitening.

Example  4. Of Prickly Pear Extract ACE  Measurement of inhibitory activity

Angiotensin I-converting enzyme (ACE) inhibitory activity was used by modifying the method by Saito et al. 100 μL of ACE (0.125 U / mL, from rabbit lung; Sigma, St. Louis, MO, USA) was added to 100 μL of the sample prepared by the concentration of Prickly Pear Extract, and incubated at 37 ° C. for 3 minutes. 150 μL of a substrate solution containing 2.5 mM HHL (hippuryl-Lhistidyl-L-leucine; Nacalai, Kyoto, Japan), 300 mM NaCl, and 100 mM borate buffer is added, and the mixture is added again at 37 ° C. for 30 minutes. Incubated. After the reaction was terminated by adding 350 μL of 1 N HCl, activity analysis was performed by extracting the dissolved manic acid (hippuric acid) from HHL by ACE. Extraction of manic acid was carried out by adding 3 mL of ethyl acetate to the reaction mixture, stirring and separating the ethyl acetate layer, and concentrating under reduced pressure. The solution was redissolved using 2 mL of distilled water and 228 nm (UV-1200; Shimadzu, Absorbance was measured in Kyoto, Japan). A calibration curve was prepared based on the measured absorbance, and the concentration of 50% inactivation concentration (IC50) was calculated. The control group was captopril (captopril, Sigma St. Louis, MO, USA), which is a commercial ACE inhibitor. Was used, and the results are shown in Table 3 below. [Table 3. ACE Inhibitory Effect of Fruits of Prickly Pear Extract (ASFE)].

Hypertension is associated with long-term diabetes and is at risk of exposure to many cardiovascular diseases. Preventing hypertension by controlling the expression of ACE using antihypertensive agents is an important strategy to lower this risk. The fact that many natural products show ACE inhibitory effect is likely to be due to the action of various phenolic compounds contained in plant resources, and flavonoids among phenolic compounds isolated from persimmon leaves increase ACE inhibitory activity. It is speculated that ACE inhibitory activity is increased due to the phenolic compound synthesized through the phenylpropanoid pathway contained in P. fiores.

Sample Captopril ASFE ACE inhibition Concentration (IC ₅₀ ㎍ / mL) 0.085 ± 0.016 29.9 ± 2.7  Mean ± S.E obtained from six experiments

As shown in Table 3, as a result of examining the ACE inhibitory activity of the fruit extract of P. falciparum extract, it did not reach the level of captopril, an antihypertensive control active substance, but the ACE inhibitory activity (IC ₅₀ ) was found to be 29.9 ㎍ / mL, indicating that XO inhibition There was a significant correlation with activity.

Example  5. Determination of Anticancer Activity of Prickly Pear Extract

The inhibitory effect of cell growth inhibition on human cancer cell lines of P. falciparum extract was investigated by SRB (Sulfo Rhodamine B) method. The cancer cell line was a prostate cancer cell line LNCaP, colon cancer cell line HCT-15, gastric cancer cell line ACHN, lung cancer cell line A549, leukemia cell line MOLT-4F was used. To confirm the toxicity to normal cells, hepatocyte line 293 (human embryo liver) was purchased from Korea Cell Line Bank (KCLB) and used as a control. The cancer cell line was cultured using RPMI 1640 medium containing 10% fetal bovine serum, and the cultured cells were maintained by dispensing once or twice a week.

The cell concentration used to measure anticancer activity is 3,000? It was 6,000 pieces / mL, and all the reagents used in the above method were dissolved in 100% DMSO, and diluted in steps to prepare sample concentrations of P. chlorophyll fruit extract at 30, 10, 5, 2.5, 1, 0.1 μg / mL. Measure the number of cells, divide them into 96-well plates at a constant concentration, and use the same cell concentration as the plate to be treated with the Tz plate needed to display the basic absorbance of the cells after one day. Another plate with was fixed using 50% TCA. The plate to be treated with the sample was treated with five concentrations by adjusting the final concentration of the sample to 0.1% DMSO. The Tz plate was washed with tap water after 1 hour, and the plate treated with the sample was fixed with 50 μL of 50% TCA per well after 2 days, and washed with tap water after 1 hour. The washed plates were dried at room temperature, then 0.4% SRB solution was added to 100 μL per well, and then washed with 1% acetic acid solution after 30 minutes, and dried again at room temperature. Next, 10 mM Tris base (pH: 10.5) was added to 100 μL per well to dissolve again, and the absorbance was measured at 540 nm using an ELISA reader. At this time, the concentration of the compound that inhibits the growth of cancer cells by 50% was expressed as GI 50 (µg / mL), and adriamycin was used as a control.

The results of the cell growth inhibitory effect on human cancer cell lines on the extract of P. chlorophyllum extract by SRB method are shown in Table 4. [Table 4. Toxic Cell Activity of P. chlorophyllum Extract (ASFE) on Human Cancer Cell Line ].

Sample GI ₅₀ (Μg / mL) LNCaP HCT-15 ACHN  A549  MOLT-4F ASFE 5 > 30 10 > 30 5 Adriamycin 0.13 0.16 0.13 <0.03 0.05

As shown in Table 4, the cytotoxicity of the cancer cell lines (LNCaP prostate cancer, HCT-15 colon cancer, ACHN kidney cancer, A549 lung cancer, MOLT-4F leukemia), lung cancer cell lines A549 cells and colon cancer cells In the host HCT-15, P. falciparum extract did not show activity, whereas LNCaP, a prostate cancer cell line, and MOLT-4F, a leukemia cell line, showed an excellent cytotoxic effect at a concentration of 5 ㎍ / mL, which inhibits the growth of cancer cells by 50%. It was.

In experiments to determine the virulence of 293, a normal hepatocyte line, the effect of P. falciparum extract on LNCaP, a leukemia cell line, and LNCaP, a leukemia cell line, were observed. Inhibition rate was around 60%, whereas growth inhibition rate was 2% or less for 293 cells, normal liver cell lines.

This can be said that the fruit extract of P. falciparum shows a high growth inhibitory effect on cancer cell lines, but little growth inhibitory effect on normal cells.

Example  6. Main Components of Prickly Pear Extract Cyanidin  3- Sambubioside ( cyanidin  3- sambubioside )) detach

As a result of assaying the physiological activity of the prepared extracts of the Prunus vulgaris, it was possible to confirm the excellent physiological activity, and the main components of these activities were identified and separated. As a result of analyzing P. falcipar fruit extract by reverse phase HPLC, major peaks were identified at retention time of 10.2 minutes, and main components were separated by preparative-HPLC using C ₁₈ column. As the separation conditions, the column was HiQ sil C18-10 (21.0 × 250mm, KYA TECH, Japan) .The analysis wavelength was 520 nm, the flow rate was 10 mL per minute, the solvent A was 5% formic acid and the solvent B was 5%. Separation was carried out at 25% B condition using formic acid-containing acetonitrile, and the sample injection amount was adjusted to 2 mL to perform high purity separation.

Figure 2 shows the HPLC chromatogram of Prunus vulgaris extract, Figure 3 shows the HPLC chromatogram of pure compounds isolated from Prunus vulgaris extract, and Figure 4 shows the UV- VIS absorbance properties are shown in chromatograms.

After absorbing the main components contained in the Prunus vulgaris, the absorbance spectra were found to be 248, 282, 326 and 518 nm. LC-ESI / MS analysis showed molecular weight m / z = 581 [M ⁺ ]. [Fig. 5], ¹ H, ¹³ C-NMR analysis results [Figs. 6 and 7, Table 5] The main components contained in the fruit of the thorny oak fruit were aglycone, and the cyanidin compound was bound, and the structural binding sugars were glucose and xylose ( xylose) was confirmed to be a cyanidin 3-sambubioside compound (Formula 1).

¹³ C-NMR ¹ H-NMR δ (ppm) J (㎐) Carbon 2 164.41 3 145.51 4 133.54     8.96 s 5 159.26 6 102.17     6.67 d 2 7 170.37 8  97.68     6.92 d 1.5 9 157.61 10 113.30 One' 121.40 2' 118.66     8.05 d 2.5 3 ' 147.53 4' 155.89 5 ' 117.50     7.03 d 9 6 ' 128.81     8.30 dd 2, 9 glucoside One" 102.17     5.43 d 7.5 2"  81.39     3.98 dd 8, 9 3 "  77.30     3.79 t, 9 4"  71.09     3.48 dd 9.5, 9.5 5 "  77.92     3.61 ddd 6.5, 6, 2 6 "  62.33     3.98 dd 2, 12     3.77 dd 6, 12 xyloside One"' 106.30     4.72 d 7.5 2"'  75.20     3.14 dd 8, 9 3 ″ ′  78.80     3.28 m 4"'  70.14     3.35 ddd 9, 10.5, 5.5 5 ″ ′  67.26     3.63 dd 5.5, 11.5     3.03 dd 10.5, 11.5

The above results are the possibility of developing as a drug that can prevent or treat gout, hypertension and cancer or improve health foods by using the compound represented by the formula (1) contained in P. It is to be implied.

The present invention described above is not limited to the above-described embodiment and the accompanying drawings, and various substitutions, modifications, and changes are possible within the scope without departing from the technical spirit of the present invention. It will be evident to those who have knowledge of.

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Claims (4)

A composition for the prevention and treatment of gout, hypertension and cancer, characterized by containing the Prickly Pear Extract as an active ingredient.
delete Health food for the prevention and improvement of gout, hypertension and cancer, characterized by containing the Prickly Pear Extract as an active ingredient.
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