KR100860540B1 - Composition for preventing or treating a neurodegenerative disease comprising active ingredients isolated from the stembark of vitis vinifera, which has inhibitory effect on bace-1 - Google Patents

Abstract

The present invention relates to a compound selected from the group consisting of vitisin B, epsilon-viniferin, ampelopsin A and mixtures thereof, or Vitis vinifera bark comprising the same. A composition comprising an extract as an active ingredient, the composition of the present invention is a BACE-1 (beta-site APP-cleaving enzyme 1 that mediates the formation of beta-amyloid, which is widely known as a causative agent of various dementia) It can be effectively used for the prevention and treatment of various degenerative brain diseases such as Alzheimer's disease, Parkinson's disease, Lou Gehrig's disease, Huntington's disease and Neiman-Pick disease.

Description

COMPOSITION FOR PREVENTING OR TREATING A NEURODEGENERATIVE DISEASE COMPRISING ACTIVE INGREDIENTS ISOLATED FROM THE STEMBARK OF VITIS VINIFERA, WHICH HAS INHI EFFECT ON BACE-1}

The present invention relates to a pharmaceutical composition for the prevention or treatment of degenerative brain diseases, which comprises as an active ingredient a vine bark extract that inhibits the activity of BACE-1 (β-secretase) and purified from it.

Dementia is defined as the gradual deterioration of memory and cognitive abilities that impair daily life, and can be divided into vascular dementia and Alzheimer's dementia. Vascular dementia mainly corresponds to a case in which cerebral infarction or stroke occurs due to a blood clot formed in a blood vessel, and it is known that brain cells around the affected area are damaged to cause symptoms such as memory loss. On the other hand, Alzheimer's dementia, which accounts for far more than vascular dementia, is a progressive, progressive brain disease with symptoms such as memory loss, changes in personality, and decreased thinking, and most patients have pneumonia within 8 to 10 years. Die from back It is estimated that between 3.5 and 10% of senior citizens over 65 years of age have this disease and there are 4 million patients in the United States alone. Is a representative senile disease. The characteristic lesions of Alzheimer-type dementia, the brain tissue beta- the like that contain the amyloid noinban (amyloid plaques), nerve fibers (neurofibrillary tangles) is formed going inflammatory reaction in the immune cells, Ca ^{2 +} channel is destroyed In addition, the decrease in the amount of the neurotransmitter acetylcholine (acetylcholine) has been reported to cause symptoms of memory loss, cognitive function. Recent epidemiologic studies have reported that risk factors for cerebrovascular diseases such as hypertension, diabetes, hyperlipidemia and heart disease increase the incidence of Alzheimer's as well as vascular dementia, but the exact etiology and treatment are not known.

 Most modern medical perspectives on cognitive deterioration due to Alzheimer's dementia consider the widespread degeneration and loss of cholinergic neurons in the brain as the main cause. The main task is to develop drugs that can partially restore the impaired cognitive function. Drugs currently approved by the US Food and Drug Administration (FDA) for the treatment of Alzheimer's dementia (tacrine, rivastigmine, donepezil, galantamine) all attempt to enhance cognitive function by inhibiting the activity of acetylcholinease. Aka acetylcholinesterase inhibitors. However, these drugs show only a temporary symptomatic effect in some Alzheimer's dementia patients (40-50%), and the drug does not last long. In addition, due to the nature of the disease requires long-term administration, the acetylcholinesterase inhibitors developed so far have been accompanied by a number of side effects, including hepatotoxicity has also been revealed as a problem.

With recent advances in genetics, cell biology, and molecular biology, the amyloid hypothesis has been proposed as a hypothesis for the pathogenesis of Alzheimer's dementia. Specifically, beta-amyloid free from the amyloid precursor protein (β- a myloid p recursor p rotein (APP)) aggregates together to produce insoluble senile plaques, which cause neuronal degeneration. Secondly, nerve fiber bundles are produced. As it has been found that the accumulation of beta-amyloid in the brain and its neurotoxicity is a very important cause of Alzheimer's dementia, it has an inhibitory effect on the formation, aggregation or toxicity of beta-amyloid. Investigation studies of small substances are being concentrated.

Beta-secretase, the enzyme that plays the most important role in the production of beta-amyloid, is commonly called BACE (beta-site APP-cleaving enzyme), and there are two types of BACE-1 and BACE-2. Known. Among them, BACE-1 has most of the activity of beta-secretase (about 90%) and is known to play a much more important role than BACE-2 in the production of beta-amyloid. Thus, the material that selectively inhibit the activity of BACE-1 are being sufficiently recognize the value that can be used as active therapeutic agents in Alzheimer type dementia (lit. [Guo T, Hobbs DW. Curr Med Chem. 13 (15): 1811-29, 2006; Thompson LA, Bronson JJ, Zusi FC. Curr Pharm Des. 11 (26): 3383-404, 2005; Cumming JN, Iserloh U, Kennedy ME. Curr Opin Drug Discov Devel . 7 (4): 536-56, 2004; Citron M. Trends Pharmacol Sci . 25 (2): 92-7, 2004 and Dewachter I, Van Leuven F. Lancet Neurol . 1 (7): 409-16, 2002).

Meanwhile, the vine ( Vitis vinifera ) is a deciduous tree belonging to the family of grapes and its fruit is a widely used food such as grape juice and wine making. It contains various organic acids such as glucose, fructose, sucrose, citric acid, tartaric acid, acetic acid and malic acid. Doing. In addition, the shell contains anthocyanin-based ingredients such as paeonidin, delphinidin, malvidin and petunidin, which act as anti-cancer effects and antioxidants. It is known. However, there are few studies on the BACE-1 inhibitory activity of vine extract to date.

It is an object of the present invention to provide a novel compound having BACE-1 inhibitory activity and a vine bark extract comprising the same.

Another object of the present invention is to provide a pharmaceutical composition for the prevention and treatment of degenerative brain diseases, which contains the extract of vine bark or the active compound isolated therefrom as an active ingredient.

Still another object of the present invention is to provide a health promoting food having a prophylactic and therapeutic effect of degenerative brain disease using the vine bark extract or the active compound isolated therefrom as an active ingredient.

In order to achieve the above object, the present invention provides a novel compound of formula (1).

In addition, a compound selected from the group consisting of the compound of Formula 1, bitisin A, bitisin B, epsilon-viniferin, ampelopsin A, and mixtures thereof or vine bark extract containing the same as an active ingredient, degenerative It provides a pharmaceutical composition for the prevention or treatment of brain diseases and foods for promoting health having the effect of preventing or treating degenerative brain diseases.

In addition, the vine bark is prepared by extracting with a solvent selected from the group consisting of methanol, aqueous methanol solution, ethanol, aqueous ethanol solution, butanol, dichloromethane, ethyl acetate and mixtures thereof, the compound of formula 1, Vitisin A It provides a vine bark extract, which comprises a compound selected from the group consisting of, bitisin B, epsilon-viniferin, ampelopsin A, and mixtures thereof, and which has a prophylactic or therapeutic effect on degenerative brain diseases.

Hereinafter, the present invention will be described in detail.

In this specification, degenerative brain disease is a disease related to neurofibrillary bundle or β-amyloid accumulation in the brain, and may include Alzheimer's disease, Parkinson's disease, Lou Gehrig's disease, Huntington's disease, Neiman-Pick's disease, etc., but is not limited thereto.

The compound of formula 1 of the present invention is purified from vine bark extract, qualitative analysis using ¹ H-NMR, ¹³ C-NMR, HMQC, HMBC and DEPT, Vitisinol C (Huang YL , Tasi WJ, Shen CC, Chen CC, J. Nat. Prod. 68: 217-220, 2005).

Vine bark extract according to the present invention can be prepared by solvent extraction of the vine bark or dried product thereof. Specifically, 2 to 200 times the volume of the vine bark, preferably 10 to 30 times the organic solvent, is added to the dry and finely cut vine bark, and 1 to 20 days at 10 to 30 ° C, preferably 5 to After extracting for 10 days, filtered and concentrated under reduced pressure, the vine bark extract can be prepared.

The organic solvent may be a solvent selected from methanol, aqueous methanol, ethanol, aqueous ethanol, and C _{1 -4} alcohol and aqueous solutions thereof, such as butanol, dichloromethane, ethyl acetate and mixtures thereof, more Preferably 80 to 100% methanol or aqueous methanol solution can be used.

Preferably, the vine bark extract obtained as described above may be reextracted with butanol, dichloromethane or ethyl acetate, and more preferably with ethyl acetate.

According to a preferred embodiment of the present invention, the vine bark or the dried product thereof is cooled for 7 days using 100% methanol, filtered and concentrated under reduced pressure to obtain a methanol extract, and the methanol extract is suspended in distilled water and the same amount of The vine bark extract can be obtained by fractionating with dichloromethane and separating the water layer with the same amount of ethyl acetate and concentrating under reduced pressure.

In addition, according to the present invention, a specific active substance having BACE-1 inhibitory ability can be isolated and purified by chromatography from vine bark extract. The chromatography is preferably subjected to reverse phase or silica gel column chromatography once or twice. As the mobile phase, dichloromethane / methanol mixture (40: 1 to 1: 1) or methanol / water mixture (20% to 100% MeOH) is used. ) Can be used. In this case, the solvent is eluted by a gradient gradient eluting method that is sequentially raised from nonpolar to polar or nonpolar to polar, and the desired active fraction can be obtained by measuring the BACE-1 inhibitory effect of the collected isolates.

In addition, the active fraction showing the inhibitory ability of BACE-1 was purified by chromatography again, according to the following formula: Vitisinol E of Formula 1, Viticin A of Formula 2, Viticin B of Formula 3, epsilon-viniprine of Formula 4, And an active ingredient such as ampelopsin A of the formula (5) can be obtained. In addition, if necessary, bitisin A, bitisin B, epsilon-viniferin, ampelopsin A and bitisinol E can be chemically synthesized.

<Formula 1>

Vine bark extract of the present invention, isolated from this, Vitisin A, Vitisin B, epsilon-viniferin, ampelopsin A, Vitisinol E or mixtures thereof are used for the treatment of degenerative brain diseases through BACE-1 inhibition. Since it shows an excellent effect, it can be used for the prevention and treatment of senile dementia.

Therefore, degenerative brain disease comprising a compound selected from the group consisting of bitisin A, bitisin B, epsilon-viniferin, ampelopsin A, bitisinol E, and mixtures thereof or vine bark extract containing the same as an active ingredient Prophylactic or therapeutic pharmaceutical compositions are also within the scope of the present invention.

As an active ingredient, extract of vine bark, Vitisin A, Vitisin B, Epsilon-Viniferin, Amfellopsin A, Vitisinol E or mixtures thereof, may be used in a suitable pharmaceutically acceptable carrier or It can be mixed with an excipient or diluted with a diluent to prepare a pharmaceutical composition having the above functions. Examples of suitable carriers, excipients and diluents include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, malditol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose , Microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. In addition, the pharmaceutical composition of the present invention may further include a filler, an anticoagulant, a lubricant, a humectant, a perfume, an emulsifier, a preservative, and the like.

The pharmaceutical compositions of the present invention may be formulated using methods well known in the art to provide rapid, sustained or delayed release of the active ingredient after administration to a mammal. The formulation may be in the form of tablets, pills, powders, sachets, elixirs, suspensions, emulsions, solutions, syrups, aerosols, soft or hard gelatin capsules, sterile injectable solutions, sterile powders and the like.

The pharmaceutical compositions of the invention can be administered via several routes including oral, transdermal, subcutaneous, intravenous or intramuscular. Typical daily dosages of the pharmaceutical compositions of the present invention, based on the active ingredient, the vine bark extract ranges from 10 to 100 mg / kg body weight, preferably from 10 to 30 mg / kg body weight, , Bitisin B, epsilon-viniferin, ampelopsin A, bitisinol E or mixtures thereof ranges from 1 to 30 mg / kg body weight, preferably from 1 to 10 mg / kg body weight, divided into one or several times May be administered. However, the actual dosage of the active ingredient should be determined in light of several relevant factors such as the route of administration, the age, sex and weight of the patient, and the severity of the disease, and therefore the dosage limits the scope of the invention in any aspect. It is not.

In addition, the present invention is a degenerative agent comprising a compound selected from the group consisting of bitisin A, bitisin B, epsilon-viniferin, ampelopsin A, bitisinol E, and mixtures thereof, or a vine bark extract comprising the same as an active ingredient. Provided is a health promoting food having an effect of preventing or treating brain diseases.

As a food to which the vine bark extract of the present invention which exhibits the above effects, Vitisin A, Vitisin B, epsilon-viniferin, ampelopsin A, bitisinol E or mixtures thereof, which are separated therefrom, may be added, for example. For example, various foods, beverages, snacks, sweets, gums, ice creams, tea bags, instant tea, granules, flavorings, vitamin complexes, and other health supplements, but are not limited thereto.

Vine bark extract of the present invention, Vitisin A, Vitisin B, epsilon-viniferin, Amfellopsin A, Vitisinol E, or mixtures thereof, is added to the raw materials in the preparation of the food or added to the cooked food. The above-mentioned food or beverage may be prepared by appropriate mixing, in which case the vine bark extract, isolated from this, vitisin A, bitisin B, epsilon-viniferin, ampel The content of Robsin A, Viticinol E or mixtures thereof may range from 0.01 to 30% by weight, respectively.

The pharmaceutical composition or food for health promotion of the present invention may further include other medicinal herbs or extracts thereof that are pharmaceutically acceptable to enhance or supplement the desired effect, and representative examples of such herbal medicines are pagoji, hungry, candle Headball, lotus root meat and cloves. The herbal medicine may be used in an amount of 0.01 to 50% by weight based on the total weight of the composition.

Hereinafter, the present invention will be described in more detail based on the following examples. However, the following examples are not intended to limit the invention only.

Example : Preparation of Vine Bark Extract and Isolation of Active Ingredients

12 kg of dried vine bark was cooled to 40 l of methanol at room temperature for 1 week, filtered and the filtrate was concentrated under reduced pressure to obtain 714 g of methanol extract. The methanol extract was suspended in 20 L of distilled water and then extracted with the same amount of dichloromethane. The water layer was reextracted with the same amount of ethyl acetate and the extract was concentrated under reduced pressure to obtain 364 g of ethyl acetate reextract. The remaining water layer was freeze-dried.

50 g of the ethyl acetate re-extract, dichloromethane / methanol mixed solution as a mobile phase at a flow rate of 3 ml / min while increasing the ratio of dichloromethane / methanol from 20: 1 to 1: 1 concentration gradient Silica gel column chromatography was performed by elution method and divided into a total of six fractions (first to sixth fractions). The fifth and six fractions were subjected to silica gel column chromatography again (dichloromethane / methanol 40: 1 to 1: 1 as a mobile phase, flow rate 3 ml / min), and a total of six fractions (parts 5-1 to 5-6 fractions). 50 mg of the new compound and 300 mg of epsilon-viniprine were separated from the 5-2 fraction, and reverse phase chromatography (20-100% methanol) was performed again with the 5-4 fraction to obtain 280 mg of Amfellopsin A, 150 mg of Bitsin A and 220 mg of Bitsin B were isolated, respectively. The separated compounds were identified by ¹ H-NMR and ¹³ C-NMR, and the novel compounds obtained from the 5-2 fraction were ¹ H-NMR, ¹³ C-NMR, heteronuclear multiple quantum coherence spectroscopy (HMQC), HMBC ( The structure was confirmed using heteronuclear multiple-bond correlation spectroscopy (DEP) and distortionless enhancement by polarzation transfer (DEPT), and was named Vitisinol E. The analysis results of the Viticinol E are shown below.

¹ H-NMR (DMSO-d6, 500 MHz): δ 7.30 (2H, d, J = 8.5 Hz, H-2b), 6.98 (1H, d, J = 16.0 Hz, H-8b), 6.85 (2H, d , J = 8.5 Hz, H-2a, 6a), 6.72 (2H, d, J = 8.5 Hz, H-3b, 5b), 6.61 (1H, d, J = 16.0 Hz, H-7b), 6.59 (2H , d, J = 8.5 Hz, H-3a, 5a), 6.34 (1H, s, H-10b), 5.92 (1H, d, J = 2.0 Hz, H-12a), 5.89 (2H, d, J = 2.0 Hz, H-10a, 14a), 4.16 (1H, s, H-13b), 3.86 (1H, d, J = 11.0 Hz, H-8a), 3.69 (3H, s, COOMe), 3.15 (1H, dd, J = 11.0, 13.0 Hz, H-7a), 2.95 (1H, dd, J = 13.0, 17.7 Hz, H-12b-b), 2.27 (1H, d, J = 17.7 Hz, H-12b-a ); ¹³ C (DMSO-d6, 125 MHz): δ 43.6 (C-7a), 47.9 (C-12b), 49.1 (C-13b), 51.6 (C-8a), 52.9 (OCH3), 100.8 (C-12a) , 105.8 (C-10a, 14a), 115.0 (C-3a, 5a), 115.7 (C-3b, 5b), 127.2 (C-1b), 128.1 (C-2a, 6a), 128.2 (C-8b) , 128.9 (C-2b, 6b), 130.3 (C-10b), 134.3 (C-7b), 134.6 (C-1a), 145.7 (C-9a), 148.0 (C-9b), 155.6 (C-4a ), 158.0 (C-11a, 13a), 158.4 (C-4b), 172.7 (C-14b), 200.9 (C = O).

Experimental Example : Confirmation of BACE- 1 Inhibitory Effect of Extracts from Vine Bark and Active Ingredients Isolated from the Bark Extract

BACE-1 FRET analysis of the BACE-1 inhibitory effects of methanol extract and ethyl acetate re-extract, bitisin A, bitisin B, epsilon-viniferin, ampellopsin A and bitisinol E of the vine bark obtained in the above example It was measured as follows using the kit (PanVera ^® ).

First, 10 μl of BACE-1 substrate solution (final concentration 250 nM) was added with 10 μl of methanol extract and ethylacetate reextract, bitisin A, bitisin B, epsilon-viniferin or ampelopsin A at the desired concentration and BACE 10 μl of the −1 enzyme solution (final concentration 0.3 unit / ml) was added and then incubated at room temperature for 60 minutes. Immediately after incubation, 10 μl of the reaction stop solution was added to the reaction solution and 545 nm (excitation) and 585 nm (excitation) using a fluorescence image analyzer (FlexStation; Molecular Devices Corporation, USA). Fluorescence was measured to determine enzyme activity. The total volume of the reaction mixture was 40 μl, run on 384 black microwell plates. Detailed compositions of enzymes, substrates and assay buffers used in the experiments are shown in Table 2 below.

BACE-1 enzyme activity was measured by fluorescence analysis of the amount of substrate degradation at the end of the enzyme reaction, and the enzyme inhibition rate of the sample was converted by comparing the enzyme activity of the control group without the sample and the enzyme activity of the test group without the sample. . First, measure the BACE-1 inhibition rate of the sample at the concentration of 10 ㎍ / ㎖ and 100 ㎍ / ㎖, and dilute the sample step by step in the concentration gradient of 7-8 steps for the sample showing 50% or more inhibitory effect at each concentration The enzyme inhibition rate was determined, and the IC ₅₀ (50% enzyme inhibition concentration) value of each sample was determined by linear regression analysis on the dose-response curve. As a result, it was confirmed that each compound inhibits the activity of BACE-1 in a concentration-dependent manner, the IC ₅₀ value of each compound is shown in Table 3 below.

As shown in Table 2, the vine bark extract, Vitisin A, Vitisin B, Epsilon-Viniferin, Ampelopsin A and Vitisinol E, which have been separated and purified therefrom, effectively inhibit BACE-1 activity. It can be expected to prevent and treat degenerative brain diseases.

In addition, the dichloromethane fraction and water fraction obtained by dividing the vine bark methanol extract with dichloromethane and water, respectively, in the above examples were used as specimens. It was confirmed that the ethyl acetate fraction (ethyl acetate reextract) showed a relatively good BACE-1 inhibitory effect compared to the dichloromethane fraction and the water fraction.

Pharmaceutical formulations and foods were prepared using the various vine bark extracts prepared in the above examples.

Formulation example  One: Powder  Produce

The powders were prepared by mixing the following ingredients and then filling the airtight cloth according to a conventional powder preparation method:

2 g of vine bark dry extract containing active ingredient

1 g lactose

Formulation example  2: preparation of tablets

The tablets were prepared by mixing the following ingredients and then tableting according to the conventional tablet preparation method:

100 mg of dried vine bark extract containing active ingredient

100 mg corn starch

Lactose 100 mg

2 mg magnesium stearate

Formulation example  3: Preparation of Capsule

The capsules were prepared by mixing the following ingredients and filling the gelatin capsules according to a conventional capsule preparation method:

100 mg of dried vine bark extract containing active ingredient

100 mg corn starch

Lactose 100 mg

2 mg magnesium stearate

Formulation example  4: Preparation of Injection

According to a conventional injection preparation method, the vine bark extract containing the active ingredient is dissolved in distilled water for injection, the pH is adjusted to about 7.5, and the following remaining ingredients are filled in 2 ml ampoules with distilled water for injection and sterilized. Injections were prepared:

100 mg of dried vine bark extract containing active ingredient

Suitable amount of distilled water for injection

pH adjuster

Formulation example  5: Linear  Produce

Brown rice, barley, glutinous rice, and yulmu were alphad by a known method, and then dried and roasted to make a powder having a particle size of 60 mesh. Black beans, black sesame seeds, and perilla were also steamed and dried in a known manner, and then ground to a powder having a particle size of 60 mesh.

The vine bark extract of the present invention was concentrated under reduced pressure in a vacuum concentrator, and the dried product obtained by drying with a spray hot air dryer was pulverized to a particle size of 60 mesh with a grinder to obtain vine bark extract dry powder.

Granules were prepared by combining the dry powders of the grains, seeds and vine bark extract prepared above in the following proportions.

Cereals: Brown rice 30% by weight, barley 15% by weight, barley 20% by weight, glutinous rice 9% by weight,

Seeds: perilla 7% by weight, black beans 8% by weight, black sesame 7% by weight,

Vine bark extract dry powder 3% by weight, ganoderma lucidum 0.5% by weight, turmeric

Formulation example  6: Chewing gum  Produce

Chewing gum was prepared in a conventional manner by combining 20% by weight of gum base, 76.9% by weight of sugar, 1% by weight of perfume, and 2% by weight of water and 0.1% by weight of vine bark extract of the present invention.

Formulation example  7: Preparation of Candy

Candy was prepared in a conventional manner by combining 60% by weight of sugar, 39.8% by weight of starch syrup, and 0.1% by weight of perfume and 0.1% by weight of the vine bark extract of the present invention.

Formulation example  8: Biscuit  Produce

Force 25.59% by weight, 22.22% by weight of gravity, 4.80% by weight, white salt 0.73% by weight, 0.78% by weight, palm shortening 11.78% by weight, ammonium 1.54% by weight, 0.17% by weight sodium bisulfite 0.16% by weight , Rice flour 1.45%, Vitamin B₁0.0001%, Vitamin B₂0.0001%, Milk flavor 0.04%, Water 20.6998%, Whole milk powder 1.16%, Substitute milk powder 0.29%, Monobasic calcium phosphate 0.03% , Biscuits were prepared in a conventional manner by combining 0.29% by weight of spraying salt and 7.27% by weight of spray oil with 1% by weight of the vine bark extract of the present invention.

Formulation example  9: Manufacture of health drinks

0.26% by weight of honey, 0.0002% by weight of thioctoamide, 0.0004% by weight of nicotinic acid, 0.0001% by weight of sodium riboflavinate, 0.0001% by weight of pyridoxine hydrochloride, 0.001% by weight of inositol, 0.002% by weight of orthoic acid and 98.7362% by weight of water 1% by weight of the extract of bark of vine was prepared in a conventional manner for a healthy drink.

Formulation example  10: Preparation of dietary supplements

55% by weight of spirulina, 10% by weight of guar gum enzyme, 0.01% by weight of vitamin B ₁ hydrochloride, 0.01% by weight of vitamin B6 hydrochloride, 0.23% by weight of DL-methionine, 0.7% by weight of magnesium stearate, 22.2% by weight of lactose and 1.85% by weight of corn starch And blended 10% by weight of the vine bark extract of the present invention to prepare a tablet-type health supplement in a conventional manner.

The composition comprising Viticin B, epsilon-viniferin, Amfellopsin A, or a mixture thereof as an active ingredient, BACE-1 (mediated formation of beta-amyloid, which is widely known as a causative agent of various dementia) Because it effectively inhibits beta-site APP-cleaving enzyme 1), it can be useful for the prevention and treatment of various degenerative brain diseases.

Claims (5)

Degenerative brain disease selected from the group consisting of Alzheimer's disease, Parkinson's disease, Lou Gehrig's disease, Huntington's disease, and Niman-Pick's disease, comprising as an active ingredient a compound selected from the group consisting of Vitisin B, epsilon-viniferin, ampelopsin A, and mixtures thereof Pharmaceutical composition for the prophylaxis or treatment of. The method of claim 1, The compound selected from the group consisting of bitisin B, epsilon-viniferin, ampelopsin A, and mixtures thereof, the vine bark comprising C _1-4 alcohol or an aqueous solution thereof, dichloromethane, ethyl acetate and mixtures thereof Pharmaceutical composition, characterized in that it is prepared by extraction with an organic solvent selected from. The method of claim 2, The compound selected from the group consisting of bitisin B, epsilon-viniferin, ampelopsin A, and mixtures thereof, the vine bark comprising C _1-4 alcohol or an aqueous solution thereof, dichloromethane, ethyl acetate and mixtures thereof After extracting with an organic solvent selected from the pharmaceutical composition, characterized in that the obtained extract is prepared by re-extracting with an organic solvent selected from the group consisting of butanol, dichloromethane and ethyl acetate. A health promoting food comprising a compound selected from the group consisting of bitisin B, epsilon-viniferin, ampelopsin A, and mixtures thereof as an active ingredient. The method of claim 4, wherein The food for health promotion, characterized in that the food is in the form of wire, chewing gum, candy, biscuits, drinks, or supplements.