KR101166778B1 - Apparatus and methods for animal ovum pick-up using abdominal cavity images - Google Patents

Abstract

The present invention relates to an animal egg harvesting and method using an animal abdominal cavity that can improve the improvement and proliferation of the animal by collecting a good egg without a lot of impact on the genetically superior female.
The present invention is equipped with a CMOS-type camera and an egg-scanning needle at the tip of the metal insertion tube to collect eggs while observing the inside of the abdominal cavity of a live animal such as a cow or a horse in real time, and is located at the rear end of the metal insertion tube. The handle is mounted to fix and control the metal insertion tube, and a camera body and an LCD window are mounted on the top of the handle, and the inside of the abdominal cavity of the animal captured by the camera can be vividly observed through the LCD window to monitor the position of the egg. The present invention provides an animal egg harvesting device using an animal abdominal cavity image, which allows an egg to be collected through a needle.
In addition, a metal insertion tube equipped with a camera at the distal end is inserted through the vagina of the animal, and a perforated wall of the animal is drilled using an incision knife mounted at the front end of the metal insertion tube, and then the animal is photographed through the camera. The present invention provides a method for collecting animal eggs using animal abdominal burns, which observes the eggs and follicles located inside the abdominal cavity in real time and operates the vacuum pump to inhale the eggs and follicles under vacuum pressure through the needle and transport and store them inside the test tube. .

Description

Apparatus and methods for animal ovum pick-up using abdominal cavity images}

The present invention relates to an animal egg harvesting apparatus and method using an animal abdominal cavity, and more particularly, to collecting an animal egg that can improve the improvement and propagation of the animal by collecting a good egg without much impact on genetically superior females And to a method.

In general, the fertilization of the animal is made by the mating of the male, there is always a problem that the male must move and mate, as well as the mating takes a considerable time or may not be successful.

To solve this problem, hands are inserted into the rectum of the animal to insert the injector into the cervix, cervix and uterus by hand sensation, and then freeze-thaw semen and fertilized egg into the female without moving the male. It is injected into the artificial insemination.

However, there was a drawback of causing a lot of damage to the female reproductive organs by the procedure only by the sense of the hand.

Therefore, recently, there have been attempts to improve and propagate genetically superior animals through in vitro fertilization by collecting eggs and sperm from living animals.

Currently, a method of collecting eggs from a live female animal is performed by attaching a needle to a probe of an ultrasound diagnostic apparatus, guiding the probe to the vagina of a female animal, and checking the follicle of the ovary by ultrasound image.

However, since the ultrasound diagnosis apparatus is an expensive device, it takes a lot of money to collect the egg of the animal, and it is very difficult to collect the egg because the ultrasound image is not clear.

The present invention has been invented in view of the above, and instead of the conventional ultrasound diagnostic equipment, a metal insertion tube having a camera built into the tip part of the animal can be collected while vividly observing the eggs of living animals. An object of the present invention is to provide an animal egg harvesting apparatus and method using an animal abdominal cavity image.

The present invention is to collect the eggs while observing the inside of the abdominal cavity of a live animal such as cattle or horses in real time,

CMOS type camera and egg collection needle are attached to the tip of the metal insertion tube, and the handle is fixed to the body of the metal insertion tube so that the metal insertion tube can be fixed and manipulated. Equipped with an LCD window, while providing a vivid observation of the inside of the abdominal cavity of the animal captured by the camera through the LCD window provides an animal egg harvesting device to find the location of the follicle of the ovary to collect the egg through the needle.

In addition, a metal insertion tube equipped with a camera at the distal end is inserted through the vaginal part of the animal, and the abdominal cavity wall of the animal is punctured using an incision knife mounted at the distal end of the metal insertion tube. The present invention provides a method for collecting an animal egg, which observes an egg and a follicle from an ovary located in the abdominal cavity in real time and operates the vacuum pump to suck the egg and the follicle fluid under vacuum pressure through a needle and transport and store it inside the test tube.

The advantages of the apparatus and method for collecting animal eggs using the animal abdominal cavity image according to the present invention are as follows.

1. The animal's ovary and follicles in the ovary can be confirmed while vividly observing the inside of the abdominal cavity of the animal, so that the egg of the animal can be easily collected.

2. The diameter of the metal insertion tube is very small, a cutting knife is mounted on the front end of the metal insertion tube, and a hole is made in the abdominal cavity wall of the animal by using the cutting knife, so that the egg is continuously without excessive impact on the animal. Can be harvested.

3. By using a vacuum pump instead of a syringe, the inside of the test tube is evacuated and animal eggs can be easily collected by suctioning the animal eggs by vacuum pressure.

4. It is possible to continuously harvest high quality animal eggs without damaging genetically superior females, thus improving animal improvement and propagation.

1 is a schematic view showing an animal egg harvesting apparatus according to an embodiment of the present invention
Figure 2 is an enlarged cross-sectional view of the front end of the metal insertion tube in Figure 1
Figure 3 is an operating state of the cutting knife and needle in Figure 2
Figure 4 is a state diagram showing the appearance of the egg collection of the actual animal using the apparatus of FIG.

Hereinafter, preferred embodiments of the present invention will be described with reference to the accompanying drawings.

1 is a schematic view showing an animal egg harvesting apparatus and a method harvesting apparatus using an animal abdominal cavity image according to an embodiment of the present invention, Figure 2 is an enlarged cross-sectional view of the front end of the metal insertion tube in FIG.

The present invention relates to an animal egg harvesting apparatus and method using an animal abdominal cavity image while directly observing the ovary 33 of a live female animal 28 using a camera 13.

The present invention is a camera 13 for imaging the ovary 33 of a live female animal 28, an egg harvesting needle 21 for collecting an animal egg using an animal abdominal image of the female animal 28, the egg transfer Catheter 20, vacuum pump 26 and the like.

The camera body is mounted inside the upper end of the handle 12 and serves to process the video captured from the camera 13 as an electric video signal, the interior of the abdominal cavity 32 and the ovary 33 and follicle 34 The shape of can be observed.

An LCD display 11 is installed at the rear of the monitor main body 10, and the LCD display 11 receives an image signal from the camera main body inside the handle 12 and visually gives the operator the cervix of the female animal 28. And the ovary 33 in the cervix and the like, the operator can check the position of the follicle 34 to be collected while looking at the LCD monitor.

The ovary 33 has a number of follicles 34, the follicle 34 is a single egg.

The LCD display unit 11 is coupled to the handle 12 by the universal joint, the angle can be adjusted in the up, down, left and right directions.

The handle 12 is located at the rear end of the metal insertion tube 16 to support the metal insertion tube 16 and to accurately insert the metal insertion tube 16 into the abdominal cavity through the vaginal portion 30 of the female animal 28. Can be aimed and inserted, the inside of the handle 12 is provided with a space for accommodating three AA-type 1.5V battery, it is to supply power to various electrical devices such as lighting from the battery.

The metal insertion tube 16 is mounted in the horizontal direction in front of the upper end of the handle 12, the metal insertion tube 16 has a predetermined length for injection into the cervix and cervix of the female animal 28, The CMOS type camera 13 is attached to the inner diameter of the distal end of the metal insertion tube 16.

The CMOS type camera 13 serves to hold an image indefinitely when photographing the camera 13 at regular intervals on a subject, and is fixed by a metal insertion tube 16 and the vaginal portion of the female animal 28. 30, the optical fiber wrapped around the camera 13 illuminates the uterus of the female animal 28 so that the camera 13 may have the cervix and cervix of the female animal 28 and the ovary in the abdominal cavity. 33).

The case of the handle 12 is equipped with an LED, the LED is connected to the battery is supplied with power, the optical fiber is connected between the LED and the camera 13, the light of the LED is transmitted through the optical fiber, the optical fiber The end of is provided so as to surround the camera 13, the light is irradiated through the camera protective cap 14 from the optical fiber.

In this case, the camera protection cap 14 not only serves to protect the camera 13, but also has a purpose to make it possible to clearly capture the image of the subject at regular intervals between the camera 13 and the subject.

Therefore, the camera 13 is kept close to the subject by the camera protection cap 14 to maintain a constant distance to ensure a minimum distance for clearly taking the image of the subject.

3 is an operational state diagram of the cutting knife and the needle in FIG. 2.

3, the cutting knife 15 is mounted on the distal end of the metal insertion tube 16 so as to be slidable in the front and rear direction while surrounding the distal end of the metal insertion tube 16, and the cutting knife 15 A blade is formed in an oblique direction on the inner surface of the distal end portion of the c) to perforate the abdominal cavity wall 32.

A slide groove 35 is formed in the metal insertion tube 16 so that the rear end of the cutting knife 15 can slide forward and backward along the slide groove 35.

The abdominal cavity wall 32 is perforated by inserting the metal insertion tube 16 through the vaginal portion 30 of the female animal 28 while the cutting knife 15 is fixed to the front of the camera protection cap 14. After the hole is formed in the abdominal cavity wall 32, the cutting knife 15 is fixed backward to the rear of the camera protective cap 14 to secure the view of the camera 13, and then through the camera 13. The ovary 33, etc. in the abdominal cavity wall is photographed.

At this time, the metal insertion tube 16 is inserted into the vaginal portion 30 of the female animal 28 in order to retract the cutting knife 15 to the rear of the camera protective cap 14, the metal insertion tube 16 The front and rear tubes.

The front and rear tubes are exposed to the vaginal portion 30 of the female animal 28 in a state where the metal insertion tube 16 is inserted into the vaginal portion 30 of the female animal 28 and is cut by a connecting rod. The incision knife 15 can be advanced back and forth by being connected to (15) and operated forward and backward through the forward and backward tubes outside the vaginal part 30 of the female animal 28.

An egg collecting tube 19 is integrally attached to the lower portion of the metal insertion tube 16, and an egg transfer catheter 20 is mounted inside the egg collecting tube 19, and the egg transfer catheter 20 is installed. An oocyte collecting needle 21 is attached to the front end of the head), and a tightening part 18 is attached to the rear end of the egg transfer catheter 20.

The handle 12 below the camera body is provided with a catheter insertion guide tube 17, and the egg harvesting needle 21 and the egg transfer catheter 20 are ovulated through the catheter insertion guide tube 17. Inserted into the collection tube 19, the operator moves the egg transfer catheter 20 inserted into the catheter insertion guide tube 17 in the front and rear direction to move the egg harvesting needle 21 to the egg collection tube 19 It may protrude out from the leading edge of or may be drawn inward.

And the fastening part 18 is detachably attached to the guide pipe 17 for catheter insertion, and the egg transfer catheter 20 can be fixed with this fastening part 18. FIG.

When the egg collecting needle 21 is introduced into the egg collecting tube 19, the tightening unit 18 is pushed back from the catheter insertion guide tube 17, thereby collecting the egg collecting needle 21 and the egg. When retracting the catheter 20 for feeding and protruding the egg collecting needle 21 to the outside of the egg collecting tube 19, the fastening part 18 is inserted into the guide tube 17 for catheter insertion, and the egg is fixed. The collection needle 21 and the egg transfer catheter 20 are advanced.

The first suction hose 22 is connected to the rear end of the egg transfer catheter 20 by a tightening unit 18, and the other end of the first suction hose 22 penetrates the plug 25 to collect eggs. It is inserted into the test tube 24.

At this time, when the inner diameter of the egg transfer catheter 20 is thinner than the diameter of the animal egg (200 ~ 400㎛), it is difficult to transfer the egg through the vacuum pump 26, the inner diameter of the egg transfer catheter 20 It is preferable to shape | mold and shape to 400 micrometers or more.

The stopper 25 is mounted on the upper part of the egg collection test tube 24 to seal the external air from entering the egg collection test tube 24, and the egg collection test tube 24 is first suctioned. Ov and follicle fluid sucked through the hose 22 is stored.

The second suction hose 23 is connected to the oocyte collecting test tube 24, and one end of the second suction hose 23 penetrates the plug 25 to be inserted into the oocyte collecting test tube 24. The other end of the second suction hose 23 is connected to the vacuum pump 26, and as the vacuum pump 26 is operated, air inside the oocyte collecting test tube 24 is collected through the second suction hose 23. Inhale and vacuum.

At this time, the egg collection test tube 24, the first and second suction hoses (22, 23), the vacuum pump 26 is detachable from the handle 12, can be mounted in a separate housing 27 and transported This is convenient.

Referring to the use method and operation state of the animal egg harvesting apparatus of the present invention according to such a configuration as follows.

Figure 4 is a state diagram showing the appearance of the eggs of the actual animal using the apparatus of FIG.

After the worker inserts his hand through the rectum 29 of the female animal 28 to catch an egg from a female animal 28 such as a cow or a horse, and grasps and fixes the uterus 31, the worker grips the device 12. Holding the metal insertion tube 16 is adjusted to insert through the vaginal part 30 of the female animal 28.

In this case, the camera 13 mounted on the inner diameter of the distal end of the metal insertion tube 16 captures the vaginal part 30 and the cervix of the female animal 28 as the metal insertion tube 16 moves. The video captured by the camera 13 is processed as an electric video signal, and the LCD display 11 receives the video signal from the camera body to visually display the vaginal part 30 and the cervix of the female animal 28 to the worker. do.

The operator controls the metal insertion tube 16 while checking the position of the metal insertion tube 16 inserted into the vaginal part 30 of the female animal 28 through the LCD display 11 of the camera body, and the metal insertion tube When 16 reaches the abdominal cavity wall 32 of the female animal 28, the incision knife 15 protruding from the distal end of the metal insertion tube 16 is stabbed into the abdominal cavity wall 32 to form a circular shape on the abdominal cavity wall 32. After the hole is formed, the front end portion of the metal insertion tube 16 is inserted into the abdominal cavity wall 32 so that the camera 13 approaches the ovary 33.

Then, while watching the video captured by the camera 13 with the LCD display unit 11 can observe the ovary 33 and the egg of the live female animal 28 inside the abdominal cavity wall 32, the egg harvesting After protruding the needle 21 to the outside of the collection tube, the needle 21 for egg collection is moved close to the circumference of the egg to suck the living egg and follicle fluid through the needle 21 for collecting the egg.

At this time, the vacuum pump 26 is operated to suck air into the egg collection test tube 24 through the second suction hose 23 to make the inside of the egg collection test tube 24 in a vacuum state, and the test tube for egg collection. Oocytes and follicular fluids are sucked through the egg harvesting needle 21 due to the pressure difference between the internal pressure of the 24 and the abdominal cavity of the female animal 28, and the follicles sucked through the egg harvesting needle 21. ) And the follicular liquid are transferred through the egg transfer catheter 20, and flow into the egg collection test tube 24 through the first suction hose 22.

10: monitor body 11: LCD display
12: knob 13: camera
14: camera protection cap 15: incision knife
16: metal insertion tube 17: catheter insertion guide tube
18: Fastener 19: Oocyte collection
20: egg transfer catheter 21: egg harvesting needle
22: first suction hose 23: second suction hose
24: test tube for egg collection 25: stopper
26: vacuum pump 27: housing
28: female animals 29: rectal
30: vagina 31: uterus
32: abdominal cavity wall 33: ovary
34: follicle 35: slide groove

Claims (3)

A handle 12 having a metal insertion tube 16 provided with a camera 13 at a front end thereof;
A camera body mounted on an upper end of the handle 12 and configured to process a video photographed from the camera 13 into an electric video signal;
An LCD display 11 installed at a rear surface of the camera body and visually showing the inside of the abdominal cavity of the female animal 28 to the operator by receiving an image signal from the camera body;
An egg harvesting needle (21) mounted to a lower portion of the metal insertion tube (16) and into which an egg and a follicular fluid are introduced into the abdominal cavity of the female animal (28);
An egg transfer catheter 20 connected to the egg harvesting needle 21 for transferring an egg and a follicle solution introduced through the egg harvesting needle 21;
An egg harvesting test tube (24) connected to the egg transfer catheter (20), in which the egg and follicle fluid transferred through the egg transfer catheter (20) are introduced and stored;
The egg collection test tube 24 is connected to the egg collection test tube 24 and sucks air in the egg collection test tube 24, and the egg and the follicle solution are collected by the vacuum pressure of the test tube 24 for egg collection. Animal egg harvesting device comprising a vacuum pump (26) to be introduced into.
2. The animal egg collection according to claim 1, wherein the cutting knife 15 is slidably mounted at the tip of the metal insertion tube 16 to pierce the abdominal cavity wall 32 of the female animal 28. Device.
After turning on the power of the camera body mounted on the upper end of the handle 12, inserting the metal insertion tube 16 provided with the camera 13 at the front end through the vaginal part 30 of the female animal 28;
Drilling the abdominal cavity wall (32) of the female animal (28) by using a cutting knife (15) protruding from the distal end of the metal insertion tube (16);
Observing the inside of the abdominal wall 32 of the female animal 28 captured by the camera 13 mounted on the metal insertion tube 16 with an LCD display 11 installed at the rear of the camera body;
After confirming the egg and follicle fluid located in the abdominal cavity wall 32 of the female animal 28, by operating the vacuum pump 26 to inhale the egg and follicle fluid through the needle 21 for collecting eggs by vacuum pressure Animal egg collection method comprising the step of transferring the egg collection test tube (24).

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