KR101164936B1 - Feed composition with hydrolyzed whey protein concentrate and feeding method thereof - Google Patents

Feed composition with hydrolyzed whey protein concentrate and feeding method thereof Download PDF

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KR101164936B1
KR101164936B1 KR1020100036483A KR20100036483A KR101164936B1 KR 101164936 B1 KR101164936 B1 KR 101164936B1 KR 1020100036483 A KR1020100036483 A KR 1020100036483A KR 20100036483 A KR20100036483 A KR 20100036483A KR 101164936 B1 KR101164936 B1 KR 101164936B1
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iron
feed composition
weight
whey protein
feeding
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KR20110116835A (en
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정현정
이성대
박준철
김상운
김인철
김상범
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대한민국
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/142Amino acids; Derivatives thereof
    • A23K20/147Polymeric derivatives, e.g. peptides or proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/189Enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/30Feeding-stuffs specially adapted for particular animals for swines
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/33Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from molasses

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Abstract

본 발명은 농축유청단백 가수분해물을 포함하는 사료조성물 및 그 급여방법에 관한 것으로, 상세하게 농축유청단백을 효소로 가수분해하여 냉동건조한 후 체내에서 이용가능한 분말을 얻어 사료로 이용하고, 그 사료를 급여하는 방법에 관한 것이다.
어미돼지 사료에 농축유청단백 가수분해물의 특정 함량을 일정량 급여함으로써, 어미돼지의 체내 미네랄 이용성과 자돈으로의 미네랄 이행을 높여 새끼돼지의 조직 및 혈액의 미네랄 이용성을 개선할 수 있도록 한 농축유청단백 가수분해물을 포함하는 철분강화 사료 조성물 및 급여방법에 관한 것이다.
The present invention relates to a feed composition comprising whey protein hydrolyzate and a method of feeding the same. Specifically, the whey protein is hydrolyzed by enzymes and lyophilized to obtain a powder that can be used in the body, and then used as a feed. It's about how to pay.
Whey protein concentrates improve the availability of minerals in the body and transfer of minerals to piglets by feeding a certain amount of whey protein hydrolyzate to the mother's pig feed to improve the pig's tissue and blood mineral availability It relates to an iron-enhanced feed composition and a method of feeding comprising a degradation product.

Description

농축유청단백 가수분해물을 포함하는 철분강화 사료 조성물 및 그 급여방법{Feed composition with hydrolyzed whey protein concentrate and feeding method thereof}Feed composition with hydrolyzed whey protein concentrate and feeding method comprising concentrated whey protein hydrolysate

본 발명은 농축유청단백 가수분해물을 포함하는 사료조성물 및 그 급여방법에 관한 것으로, 상세하게 농축유청단백을 효소로 가수분해하여 냉동건조한 후 체내에서 이용가능한 분말을 얻어 사료로 이용하고, 그 사료를 급여하는 방법에 관한 것이다.The present invention relates to a feed composition comprising whey protein hydrolyzate and a method of feeding the same. Specifically, the whey protein is hydrolyzed by enzymes and lyophilized to obtain a powder that can be used in the body, and then used as a feed. It's about how to pay.

어미돼지 사료에 농축유청단백 가수분해물의 특정 함량을 일정량 급여함으로써, 어미돼지의 체내 미네랄 이용성과 자돈으로의 미네랄 이행을 높여 새끼돼지의 조직 및 혈액의 미네랄 이용성을 개선할 수 있도록 한 농축유청단백 가수분해물을 포함하는 철분강화 사료 조성물 및 급여방법에 관한 것이다.Whey protein concentrates improve the availability of minerals in the body and transfer of minerals to piglets by feeding a certain amount of whey protein hydrolyzate to the mother's pig feed to improve the pig's tissue and blood mineral availability It relates to an iron-enhanced feed composition and a method of feeding comprising a degradation product.

돼지에 있어 가장 흔하게 부족 또는 결핍될 수 있는 광물질은 철분이다. 철분의 결핍으로 일어나는 증상은 빈혈이며, 이것은 포유자돈에서 주로 발생되어 성장을 지연시키는 주된 원인이 된다. 이는 태반으로부터 전달되는 철의 양이 제한적이고 모유에서의 철분 함량도 낮기 때문이다. 이유자돈은 적혈구와 체중이 빠르게 증가하기 때문에 철의 요구량도 높다. 모유에 철의 농도가 낮아 신생자돈의 철 저장량이 돼지의 성장을 빠르게 하고, 혈액의 용적을 증가시키는데 충분하지 않다. 따라서, 신생자돈의 철 결핍을 방지하기 위해 외부로부터 철을 공급할 수 있는 방법을 많이 이용하고 있다. 포유시기와 비슷하게 신생자돈단계는 혈액 용적이 증가하고 성장이 빠르게 이루어지는 단계이다. 가축사양표준인 NRC(1998)에서는 철의 요구량을 80㎎/㎏으로 정하고 있다.Iron is the most common deficiency or deficiency in pigs. Anemia caused by iron deficiency is anemia, which occurs mainly in mammalian pigs and is a major cause of growth retardation. This is because the amount of iron delivered from the placenta is limited and the iron content in breast milk is low. Weaning pigs have high iron demand because of the rapid increase in red blood cells and weight. Due to the low iron concentration in breast milk, iron storage of newborn piglets is not sufficient to speed up pig growth and increase blood volume. Therefore, in order to prevent iron deficiency of newborn piglets, many methods that can supply iron from outside are used. Similar to the mammalian stage, the neonatal piglet stage is a stage in which blood volume increases and growth occurs rapidly. NRC (1998), a livestock specification standard, sets iron demand to 80 mg / kg.

유청 단백질은 우유 단백질의 약 20%를 구성하며, 유제품 단백질 중에서 가용성 등급으로 분류되는데, 다양한 세포 내에서 글루타치온의 생성을 촉진하며 근육의 글루타민 저장소를 보존하는 방법으로 면역기능을 조절하는 것으로 알려져 있다. 사람의 경우, 유청단백질이 포함되어 있는 식단을 섭취할 경우 면역성이 강화된다고 알려져 있다.Whey protein makes up about 20% of milk protein and is classified as a soluble grade among dairy proteins. It is known to regulate immune function by promoting the production of glutathione in various cells and preserving muscle glutamine stores. In humans, eating a diet that contains whey protein is known to enhance immunity.

이에 농축된 유청단백질을 효소에 의해 가수분해시켜 체내에서 철분의 이용성을 높여주는 사료조성물을 제조하여 사료첨가제로 이용하고자 한다.The whey protein is hydrolyzed by the enzyme to prepare a feed composition that improves the availability of iron in the body to use as a feed additive.

본 발명은 상술한 문제점을 해결하기 위해 안출된 것으로, 본 발명의 목적은 체내 철분의 이용성을 높여 철분 강화의 효과가 있는 농축유청단백 가수분해물을 함유하는 철분강화 사료조성물을 제공하는 것이다.The present invention has been made to solve the above problems, an object of the present invention is to provide an iron-enhanced feed composition containing whey protein hydrolyzate which has the effect of iron strengthening to increase the availability of iron in the body.

본 발명의 일 측면에 따르면, 철분강화용 가축사료조성물로서, 농축유청단백과 트립신을 50:1의 비율로 혼합하여 20% 가수분해하여 동결건조된 농축유청단백질의 가수분해물 및 옥수수, 대두박, 밀기울, 당밀, 석회석, 라이신, 비타민, 미네랄 프리믹스, 인산칼슘, 효소제를 포함하는 일반 가축 기초사료 조성물을 포함하며, 상기 농축유청단백질의 가수분해물은 전체 사료조성물 100중량부에 대하여 0.01~20중량부를 포함한다. According to an aspect of the present invention, as a livestock feed composition for iron fortification, hydrolyzate and corn, soybean meal, bran of concentrated whey protein and lyophilized protein by mixing 20% hydrolyzed whey protein and trypsin in a ratio of 50: 1 , Molasses, limestone, lysine, vitamins, mineral premix, calcium phosphate, and general animal basic feed composition comprising an enzyme, wherein the hydrolyzate of concentrated whey protein comprises 0.01 to 20 parts by weight based on 100 parts by weight of the total feed composition do.

이때, 기초사료 조성물은 옥수수 60~70중량%, 대두박 10~20중량%, 당밀 0.5~2중량%, 대두유 1~3중량%, 밀기울 1~5중량%, 석회석 0.01~1중량%, 인산칼슘 0.01~2중량%, 소금 0.01~1중량%, 라이신 0.01~1중량%, 효소제 0.01~1중량%, 비타민 및 미네랄 프리믹스 0.01~1중량%을 포함할 수 있다. At this time, the basic feed composition is 60 to 70% by weight of corn, soybean meal 10 to 20% by weight, molasses 0.5 to 2% by weight, soybean oil 1 to 3% by weight, bran 1 to 5% by weight, limestone 0.01 to 1% by weight, calcium phosphate 0.01 to 2% by weight, 0.01 to 1% by weight of salt, 0.01 to 1% by weight of lysine, 0.01 to 1% by weight of enzyme, and 0.01 to 1% by weight of vitamin and mineral premix.

또한, 트립신은 돼지췌장에서 분리된 트립신일 수 있다.
The trypsin may also be trypsin isolated from the pig pancreas.

본 발명의 다른 측면에 따르면, 철분강화용 기초사료 조성물을 어미돼지에게 급여하는 방법으로, 분만 전까지 2.0~2.3kg/일을 공급하고, 분만 후부터 분만 후 7일째까지 1.2~1.5kg/일+{(0.4~0.5)×n}kg/일을 제공하며, 분만 후 8일부터는 3.8~4.0kg/일+(0.25×m)kg/일을 공급하고, 상기 n은 1≤n≤7이며, 상기 m은 상기 어미돼지 한배의 새끼돼지 마리 수인 철분강화용 기초사료 조성물을 어미돼지에게 급여하는 방법을 제공한다.According to another aspect of the present invention, a method of feeding iron fortified basic feed composition to the mother pig, supply 2.0 ~ 2.3kg / day before delivery, 1.2 ~ 1.5kg / day after delivery to 7 days after delivery + { (0.4-0.5) × n} kg / day, and from 8 days after delivery, 3.8-4.0 kg / day + (0.25 × m) kg / day, where n is 1 ≦ n ≦ 7, m provides a method of feeding the mother pig the basic feed composition for iron reinforcement that is the number of piglets one mother pig.

이때, 상기 철분강화용 기초사료 조성물을 어미돼지에게 급여하여 새끼돼지에게 철분을 공급할 수 있다. At this time, the iron fortified basic feed composition may be fed to the mother pig to supply iron to the piglets.

본 발명에 의하면, 축산업 분야에서 사용되는 기초사료에 분말형태의 가수분해된 농축유청단백을 배합함으로써, 가축을 사육함에 있어 빈혈을 방지하고, 철의 이용성을 높여 가축의 면역능력을 향상시키며, 건강한 가축을 생산할 수 있다.According to the present invention, by combining the hydrolyzed whey protein powder in the form of powder to the basic feed used in the livestock industry, to prevent anemia in raising livestock, improve the availability of iron to improve the immune ability of the livestock, Can produce livestock

도 1은 효소에 의해 가수분해시킨 후 분쇄한 농축유청단백을 나타낸 것이다.
도 2는 임신돈의 분만직후 초유의 철분함량을 나타낸 것이다.
도 3은 갓 태어난 자돈의 간에서의 철분함량을 나타낸 것이다.
도 4는 분만돈과 자돈의 분만직후(0일)과 3일 경과후 적혈구의 변화를 나타낸 것이다.
도 5는 분만돈과 자돈의 혈액에서의 철분함량을 나타낸 것이다.
도 6은 분만돈과 자돈의 혈액에서의 Total iron binding capacity를 나타낸 것이다.
도 7은 신생자돈의 혈액에서의 헤모글로빈의 농도변화를 나타낸 것이다.
Figure 1 shows the concentrated whey protein hydrolyzed by enzymes and then ground.
Figure 2 shows the iron content of colostrum immediately after delivery of pregnant pigs.
Figure 3 shows the iron content of the newborn piglets in the liver.
Figure 4 shows the change in erythrocytes immediately after delivery (day 0) and three days after the birth and piglets.
Figure 5 shows the iron content in the blood of delivery pigs and piglets.
Figure 6 shows the total iron binding capacity in the blood of delivery pigs and piglets.
Figure 7 shows the concentration change of hemoglobin in the blood of newborn piglets.

이하, 첨부된 도면을 참조로 본 발명의 실시예를 상세히 설명한다.
Hereinafter, with reference to the accompanying drawings will be described an embodiment of the present invention;

본 발명에 따른 가축사료는 가수분해된 농축유청단백질을 가축의 건강상태에 따라 해당 가축사료에 일정비율로 배합할 수 있다.Livestock feed according to the present invention can be blended hydrolyzed whey protein in a certain proportion to the animal feed according to the health of the livestock.

각 실시예에서는 임신 모돈의 배합사료에 있어서 상기 농축유청단백질의 효능을 검증하고자 실시하였으며, 이하 본 발명은 다음의 실시예에 의거하여 더 상세히 설명하며, 단 본 발명이 다음의 실시예에 의하여 한정되는 것으로 해석되어서는 안 된다.
Each embodiment was carried out to verify the efficacy of the concentrated whey protein in the combination feed of pregnant sows, the present invention will be described in more detail based on the following examples, provided that the present invention is limited by the following examples It should not be interpreted as.

실시예 1: 유청단백가수분해물 제조방법
Example 1 Whey Protein Hydrolyzate Preparation

85% 농축유청단백을 돼지췌장으로부터 분리한 효소 트립신(trypsin)을 농축유청단백: 트립신을 50:1의 비율로 혼합하여 가수분해한다. 이때 가수분해는 50℃에서 3시간 동안 이루어지며, pH는 8로 유지하고, 또한, 가수분해가 잘 되도록 교반하여 준다. 가수분해 후 가수분해율은 약 20%가 되도록 해야 하며, 가수분해율은 SDS-PAGE로 확인한다. 최종 가수분해물을 원심분리하여 가수분해가 되지 않은 산물들은 원심분리하여 제거한 후 가수분해물을 동결건조하여 분말화한 후 사료로 이용한다. 이것을 사료로 이용할 때는 단백질이므로, 손에 묻지 않도록 장갑을 끼고 배합을 하여야 한다.
Enzyme trypsin from 85% whey protein isolate from porcine pancreas is hydrolyzed by mixing whey protein: trypsin in a ratio of 50: 1. In this case, the hydrolysis is carried out at 50 ° C. for 3 hours, and the pH is maintained at 8, and the mixture is stirred to allow for good hydrolysis. After hydrolysis, the hydrolysis rate should be about 20%, and the hydrolysis rate is confirmed by SDS-PAGE. The final hydrolyzate is centrifuged to remove products that are not hydrolyzed by centrifugation, and the hydrolyzate is lyophilized to powder and then used as feed. Since it is a protein when used as a feed, it should be mixed with gloves to prevent contact with hands.

실험예 1: 농축유청단백 가수분해물의 사료조성물 제조 및 급여 방법Experimental Example 1 Preparation and Feeding Method of Feed Composition of Whey Protein Concentrate

교잡종(Yorkshire×Landrace) 임신 모돈 8두를 공시하였다. 시험설계는 표 1과 같이 옥수수-대두박 위주의 기초사료를 대조구, 가수분해된 농축유청단백 가수분해물을 제조하여 첨가한 구 2개구로 처리하여 처리당 4반복, 반복당 1두씩 완전임의 배치하였다.Eight pregnant sows of hybrid (Yorkshire × Landrace) were disclosed. As shown in Table 1, the corn-based soybean meal-based feed was treated with two spheres added with a control, hydrolyzed whey protein hydrolyzate, and added four times per treatment and one head per repetition.

표 1에서 제시한 기초사료는 NRC(1998) 사양표준을 기초로 하여 3,400kcal DE'kg, 14.5% 조단백질, 0.92% 라이신, 0.85% 칼슘, 0.60% 인을 함유하도록 하였다. 시험사료는 가루사료의 형태로 급여하였으며, 물은 자동급수기를 이용하여 자유로이 먹을 수 있도록 하였다.Based on the NRC (1998) specification, the basic feeds listed in Table 1 contain 3,400 kcal DE'kg, 14.5% crude protein, 0.92% lysine, 0.85% calcium, and 0.60% phosphorus. The test feed was fed in the form of powder feed, and the water was freely eaten by using an automatic water dispenser.

상기 어미돼지는 제조된 사료를 제한 급여하였고, 물은 자동급수기를 이용하여 자유로이 섭취할 수 있도록 하였다. 가수분해 농축유청단백질 사료는 기호성을 고려하여 1.00중량부로 첨가한 사료를 분만 전까지 2.0~2.3kg/일, 분만 후부터 7일까지는 1.2~1.5kg에서 시작하여 매일 0.4~0.5kg씩 급여량을 증가시켰고, 분만 후 8일째부터는 급여량을 3.8~4.0kg에 한배의 새끼돼지 마리 수를 0.25kg을 곱한 값만큼 추가로 급여하였다. 기타 사양관리는 국립축산과학원의 관리방법에 준하여 사육하였다.
The mother pig was given a limited feed, and the water was freely ingested using an automatic water dispenser. Hydrolysed whey protein feed increased the feed by 0.4-0.5kg daily, starting at 2.0-2.3kg / day before delivery and 1.2-1.5kg before delivery and 7 days after delivery, taking into account palatability. From the 8th day after delivery, the amount of feed was further increased by multiplying the number of single piglets by 0.25kg by 3.8 ~ 4.0kg. Other specification management was raised in accordance with the management method of the National Institute of Animal Science.

구분division 실시예 2(중량부)Example 2 (parts by weight) 비교예(중량부)Comparative example (parts by weight) 옥수수corn 69.6069.60 69.6069.60 대두박Soybean meal 19.7519.75 19.7519.75 당밀molasses 1.401.40 1.401.40 대두유Soybean oil 2.502.50 2.502.50 밀기울bran 2.002.00 2.002.00 석회석Limestone 0.800.80 0.800.80 인산칼슘Calcium phosphate 1.401.40 1.401.40 소금Salt 0.300.30 0.300.30 라이신Lysine 0.280.28 0.280.28 효소제Enzyme 0.200.20 0.200.20 비타민+미네랄 프리믹스Vitamin + mineral premix 0.690.69 0.690.69 항생제Antibiotic 0.080.08 0.080.08 탄산칼슘Calcium carbonate -- 1.001.00 농축유청단백 가수분해물Whey Protein Hydrolyzate 1.001.00 -- 합계Sum 100.00100.00 100.00100.00

상기 실시예 2와 비교예로 제조된 사료를 급여하여 사육한 임신모돈으로부터 분만 당일에 혈액과 초유를 채취하였다. 혈액 채취는 K2 EDTA 처리된 10ml 튜브를 이용하여 혈액을 채취하여 Iron, Total iron binding capacity, IgG, RBC, Hemoglobin을 분석하였다.
Blood and colostrum were collected on the day of delivery from pregnant sows fed the feed prepared in Example 2 and Comparative Example. Blood collection was performed using a 10 ml tube treated with K 2 EDTA to analyze the iron, total iron binding capacity, IgG, RBC, Hemoglobin.

실험예 2: 초유와 자돈 조직의 철분함량 분석Experimental Example 2 Analysis of Iron Contents of Colostrum and Piglets

농축유청단백 가수분해물의 이행정도를 확인하기 위하여 초유와 갓 태어난 자돈의 조직 중 간의 철분함량을 조사하였다. 초유는 분만 후 6시간 이내의 모유를 채취하였고, 신생자돈은 초유를 섭취하지 않은 자돈을 도축하여 간을 채취하였다. 그 경과 도 2 및 3에 나타난 바와 같이, 초유의 철분함량이 실시예 2에서 높았으며, 신생자돈의 간의 철분함량도 실시예 2에서 높았다.
The iron content of the tissues of colostrum and newborn piglets was examined to determine the degree of migration of Whey Protein Hydrolyzate. Colostrum was collected within 6 hours after delivery, and newborn piglets were slaughtered by slaughtered piglets that did not consume colostrum. 2 and 3, the iron content of colostrum was high in Example 2, and the iron content of newborn piglets was also high in Example 2. As shown in FIGS.

실험예 3: 혈구 수의 변화 분석Experimental Example 3: Analysis of Change in Blood Cell Count

사료내 농축유청 단백 가수분해물의 첨가로 인한 혈구 수의 변화에 미치는 영향을 도 4에 나타내었다. 도 4에서 보는 바와 같이, 분만 직후와 3일 후의 변화를 조사한 결과 실시예 2가 비교예에 비하여 적혈구(RBC) 함량이 증가하였다.
The effect on the change of blood cell count by the addition of whey protein hydrolyzate in the feed is shown in FIG. 4. As shown in FIG. 4, as a result of examining changes immediately after delivery and 3 days later, red blood cell (RBC) content was increased in Example 2 compared to the comparative example.

실험예 4: 혈청의 철분함량과 TIBC 분석Experimental Example 4: Serum Iron Content and TIBC Analysis

도 5 및 6에서 보는 바와 같이, 모돈과 신생자돈의 혈액에서 철분함량과 TIBC(total iron binding capacity)를 분석한 결과, 비교예보다 실시예 2에서 함량이 높게 나타났다. TIBC는 철 결합능을 의미하는 것으로, transferrin의 함량을 간접적으로 측정하는 방법이다. TIBC 함량이 높다는 것은 간에서 transferrin의 생산이 증가혔다는 것을 의미하므로, 실시예 2에서 첨가된 가부분해된 농축유청단백질이 모돈과 자돈이 체내 철분함량을 높이는데 효과가 있음을 보여준다.
As shown in Figures 5 and 6, the iron content and total iron binding capacity (TIBC) analysis in the blood of sows and newborn piglets, the content was higher in Example 2 than the comparative example. TIBC refers to iron binding capacity and is an indirect method of measuring the content of transferrin. Higher TIBC content means increased production of transferrin in the liver, indicating that the partially depleted whey protein added in Example 2 is effective in raising sows and piglets in the body.

실험예 5: 헤모글로빈의 농도 분석Experimental Example 5: Analysis of Hemoglobin Concentration

신생자돈의 혈액에서 헤모글로빈의 농도를 분석한 결과, 도 7에서 보는 바와 같이, 비교예보다 실시예 2에서 함량이 높게 나타나 17% 개선되는 결과가 나타났다.As a result of analyzing the concentration of hemoglobin in the blood of newborn piglets, as shown in FIG. 7, the content was higher in Example 2 than in Comparative Example, resulting in a 17% improvement.

Claims (5)

철분강화용 가축사료조성물로서,
농축유청단백과 트립신을 50:1의 비율로 혼합하여 20% 가수분해하여 동결건조된 농축유청단백질의 가수분해물 및
옥수수, 대두박, 밀기울, 당밀, 석회석, 라이신, 비타민, 미네랄 프리믹스, 인산칼슘, 효소제를 포함하는 일반 가축 기초사료 조성물을 포함하며,
상기 농축유청단백질의 가수분해물은 전체 사료조성물 100중량부에 대하여 0.01~20중량부를 포함하는 철분강화용 가축사료조성물.
As animal feed composition for iron reinforcement,
Hydrolyzate of lyophilized concentrated whey protein by 20% hydrolysis by mixing whey protein and trypsin in a ratio of 50: 1
General livestock feed composition comprising corn, soybean meal, bran, molasses, limestone, lysine, vitamins, mineral premixes, calcium phosphate, enzymes,
The hydrolyzate of the whey protein concentrate is iron for livestock feed composition containing 0.01 to 20 parts by weight based on 100 parts by weight of the total feed composition.
제 1항에 있어서,
상기 기초사료 조성물은 옥수수 60~70중량%, 대두박 10~20중량%, 당밀 0.5~2중량%, 대두유 1~3중량%, 밀기울 1~5중량%, 석회석 0.01~1중량%, 인산칼슘 0.01~2중량%, 소금 0.01~1중량%, 라이신 0.01~1중량%, 효소제 0.01~1중량%, 비타민 및 미네랄 프리믹스 0.01~1중량%을 포함하는 철분강화용 기초사료 조성물.
The method of claim 1,
The basic feed composition is corn 60-70% by weight, soybean meal 10-20% by weight, molasses 0.5-2%, soybean oil 1-3%, bran 1-5%, limestone 0.01-1% by weight, calcium phosphate 0.01 Basic feed composition for iron reinforcement comprising-2% by weight, 0.01-1% by weight of salt, 0.01-1% by weight of lysine, 0.01-1% by weight of enzyme, 0.01-1% by weight of vitamin and mineral premix.
제1항에 있어서,
상기 트립신은 돼지췌장에서 분리된 트립신인 철분강화용 기초사료 조성물.
The method of claim 1,
The trypsin is a basic feed composition for iron reinforcement is trypsin isolated from the pig pancreas.
제1항 내지 제3항 중 어느 한 항에 따른 철분강화용 기초사료 조성물을 어미돼지에게 급여하는 방법으로,
분만 전까지 2.0~2.3kg/일을 공급하고,
분만 후부터 분만 후 7일째까지 1.2~1.5kg/일+{(0.4~0.5)×n}kg/일을 제공하며,
분만 후 8일부터는 3.8~4.0kg/일+(0.25×m)kg/일을 공급하고,
상기 n은 1≤n≤7이며,
상기 m은 상기 어미돼지 한배의 새끼돼지 마리 수인 철분강화용 기초사료 조성물을 어미돼지에게 급여하는 방법.
Claims 1 to 3 of the iron feed base composition for strengthening according to any one of the way to feed the mother pig,
Supply 2.0-2.3kg / day before delivery,
1.2 to 1.5 kg / day + {(0.4 to 0.5) × n} kg / day from postpartum to 7 days postpartum,
From 8 days after delivery, 3.8-4.0 kg / day + (0.25 × m) kg / day,
N is 1 ≦ n ≦ 7,
The m is a method of feeding the mother pig the basic feed composition for iron reinforcement of the number of piglets one mother pig.
제4항에 있어서,
상기 제1항 내지 제3항 중 어느 한 항에 따른 철분강화용 기초사료 조성물을 어미돼지에게 급여하여 새끼돼지에게 철분을 공급하는 철분강화용 기초사료 조성물을 어미돼지에게 급여하는 방법.
The method of claim 4, wherein
A method of feeding a mother strengthening basic feed composition for feeding iron to iron pigs by feeding the iron pigs the basic feed composition according to any one of claims 1 to 3 to the mother pigs.
KR1020100036483A 2010-04-20 2010-04-20 Feed composition with hydrolyzed whey protein concentrate and feeding method thereof KR101164936B1 (en)

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